Ift27 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ift27
Entrez Gene ID	67042
Full Name	intraflagellar transport 27
Synonyms	2600013G09Rik,Rabl4
General protein information	Preferred Names intraflagellar transport 27 Names intraflagellar transport protein 27 homolog RAB, member of RAS oncogene family-like 4 intraflagellar transport 27 homolog rab-like protein 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_025931.3	NP_080207.1	intraflagellar transport protein 27 homolog
NM_025931.2	NP_080207.1	intraflagellar transport protein 27 homolog

Pathways from BioSystems

Homologies

Homo sapiens (human)	IFT27	NP_001171172.1
Pan troglodytes (chimpanzee)	IFT27	XP_515106.4
	LOC697032	XP_002798429.1
Bos taurus (cattle)	IFT27	NP_001069913.1
Gallus gallus (chicken)	IFT27	NP_001264309.1
Xenopus tropicalis (tropical clawed frog)	ift27	NP_001135473.1
Canis lupus familiaris (dog)	IFT27	XP_531746.3
Danio rerio (zebrafish)	ift27	NP_001008588.1
Mus musculus (house mouse)	Ift27	NP_080207.1
Rattus norvegicus (Norway rat)	Ift27	NP_001123967.1

Gene Ontology
Bibliography

Related articles in PubMed

Intraflagellar transporter protein (IFT27), an IFT25 binding partner, is essential for male fertility and spermiogenesis in mice.
Zhang Y, Liu H, Li W, Zhang Z, Shang X, Zhang D, Li Y, Zhang S, Liu J, Hess RA, Pazour GJ, Zhang Z
Developmental biology432(1)125-139(2017 Dec)

IFT25, an intraflagellar transporter protein dispensable for ciliogenesis in somatic cells, is essential for sperm flagella formation.
Liu H, Li W, Zhang Y, Zhang Z, Shang X, Zhang L, Zhang S, Li Y, Somoza AV, Delpi B, Gerton GL, Foster JA, Hess RA, Pazour GJ, Zhang Z
Biology of reproduction96(5)993-1006(2017 May)

IFT56 regulates vertebrate developmental patterning by maintaining IFTB complex integrity and ciliary microtubule architecture.
Xin D, Christopher KJ, Zeng L, Kong Y, Weatherbee SD
Development (Cambridge, England)144(8)1544-1553(2017 04)

Cilia gene mutations cause atrioventricular septal defects by multiple mechanisms.
Burnicka-Turek O, Steimle JD, Huang W, Felker L, Kamp A, Kweon J, Peterson M, Reeves RH, Maslen CL, Gruber PJ, Yang XH, Shendure J, Moskowitz IP
Human molecular genetics25(14)3011-3028(2016 07)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings suggest that like IFT25, IFT27, even though not required for ciliogenesis in somatic cells, is essential for sperm flagella formation, sperm function, and male fertility in mice. IFT25 and IFT27 control sperm formation/function through many common mechanisms, but IFT25 has additional roles beyond IFT27.
Title: Intraflagellar transporter protein (IFT27), an IFT25 binding partner, is essential for male fertility and spermiogenesis in mice.

The study implicates Ift27 in basal mechanosensation.
Title: Systems genetic and pharmacological analysis identifies candidate genes underlying mechanosensation in the von Frey test.

this study demonstrated an essential role of Ift27 in mediating hedgehog signaling during follicular keratinocyte proliferation and differentiation but not the differentiation program of the interfollicular epidermis.
Title: Intraflagellar transport 27 is essential for hedgehog signaling but dispensable for ciliogenesis during hair follicle morphogenesis.

Lztfl1 accumulate to high levels in Ift27 mutant cilia.
Title: IFT27 links the BBSome to IFT for maintenance of the ciliary signaling compartment.

IFT27 prevents aggregation of nucleotide-free ARL6 in solution
Title: The intraflagellar transport protein IFT27 promotes BBSome exit from cilia through the GTPase ARL6/BBS3.

The following Ift27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ift27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01289	NM_025931.3 Latest version!	Mus musculus intraflagellar transport 27 (Ift27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OMu01289	NM_025931.2	Mus musculus intraflagellar transport 27 (Ift27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ift27 ( NM_025931.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080207.1 Mus musculus intraflagellar transport 27 (Ift27), mRNA.

Price & Availability↑

CloneID	OMu01289
Clone ID Related Accession (Same CDS sequence)	NM_025931.3 , NM_025931.2
Accession Version	NM_025931.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	intraflagellar transport protein 27 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC017514.1 and CO039411.1. On Jul 12, 2018 this sequence version replaced NM_025931.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI412289.1, BC017514.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN01164141 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGAAGC TAGCTGCCAA ATGCATCCTG GCAGGGGACC CAGCCGTAGG CAAGACGGCC 
CTGGTGCAGA TGTTCCGCAG CGACGGGACC CATTTCCAGA AGAACTACAC CCTGACAACT 
GGAGTGGATT TGGTGGTGAA GACAGTGCCA GTTCTTGACA CAAATGACAG TGTGGAACTC 
TTCATTTTTG ACTCAGCCGG CAAGGAGCTG TTCTCTGAAA TGCTGGATAA GTTGTGGGAG 
AATCCCAACG TCCTGTGCCT TGTCTATGAT GTGACCAATG AACAGTCCTT CATCAGCTGC 
ACCAAGTGGT TGGAGAAGGT CCGGTCTCAG ACTTCAGGCA TCTCCCTCCC AGGTGTTTTA 
GTGGGAACTA AGACAGACCT GGCTGGCCGA CAAACAGTGG ATTCAGCTCA GGCCCAGGTA 
TGGGCACTGA GTCAAGGCCT GGAATTCTTT GAGACATCTG TGAAGGAGAT GGATAATTAC 
GAGGCCCCGT TCCACTGTCT GGCCAAGCAG TTCTACCAGC TGTACCGGGA AAAAGTGGAC 
ATATTCCATA CCCTGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080207.1
CDS	280..840
Translation	MVKLAAKCILAGDPAVGKTALVQMFRSDGTHFQKNYTLTTGVDLVVKTVPVLDTNDSVELFIFDSAGKELFSEMLDKLWENPNVLCLVYDVTNEQSFISCTKWLEKVRSQTSGISLPGVLVGTKTDLAGRQTVDSAQAQVWALSQGLEFFETSVKEMDNYEAPFHCLAKQFYQLYREKVDIFHTLV

Target ORF information:

RefSeq Version	NM_025931.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus intraflagellar transport 27 (Ift27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025931.3

ORF Insert Sequence:

ATGGTGAAGC TAGCTGCCAA ATGCATCCTG GCAGGGGACC CAGCCGTAGG CAAGACGGCC 
CTGGTGCAGA TGTTCCGCAG CGACGGGACC CATTTCCAGA AGAACTACAC CCTGACAACT 
GGAGTGGATT TGGTGGTGAA GACAGTGCCA GTTCTTGACA CAAATGACAG TGTGGAACTC 
TTCATTTTTG ACTCAGCCGG CAAGGAGCTG TTCTCTGAAA TGCTGGATAA GTTGTGGGAG 
AATCCCAACG TCCTGTGCCT TGTCTATGAT GTGACCAATG AACAGTCCTT CATCAGCTGC 
ACCAAGTGGT TGGAGAAGGT CCGGTCTCAG ACTTCAGGCA TCTCCCTCCC AGGTGTTTTA 
GTGGGAACTA AGACAGACCT GGCTGGCCGA CAAACAGTGG ATTCAGCTCA GGCCCAGGTA 
TGGGCACTGA GTCAAGGCCT GGAATTCTTT GAGACATCTG TGAAGGAGAT GGATAATTAC 
GAGGCCCCGT TCCACTGTCT GGCCAAGCAG TTCTACCAGC TGTACCGGGA AAAAGTGGAC 
ATATTCCATA CCCTGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ift27 ( NM_025931.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080207.1 Mus musculus intraflagellar transport 27 (Ift27), mRNA.

Price & Availability↑

CloneID	OMu01289
Clone ID Related Accession (Same CDS sequence)	NM_025931.3 , NM_025931.2
Accession Version	NM_025931.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Mus musculus(house mouse)
Product	intraflagellar transport protein 27 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC017514.1. On Jan 15, 2003 this sequence version replaced NM_025931.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC017514.1, AK011196.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN01164141 [ECO:0000348] ##Evidence-Data-END##

ATGGTGAAGC TAGCTGCCAA ATGCATCCTG GCAGGGGACC CAGCCGTAGG CAAGACGGCC 
CTGGTGCAGA TGTTCCGCAG CGACGGGACC CATTTCCAGA AGAACTACAC CCTGACAACT 
GGAGTGGATT TGGTGGTGAA GACAGTGCCA GTTCTTGACA CAAATGACAG TGTGGAACTC 
TTCATTTTTG ACTCAGCCGG CAAGGAGCTG TTCTCTGAAA TGCTGGATAA GTTGTGGGAG 
AATCCCAACG TCCTGTGCCT TGTCTATGAT GTGACCAATG AACAGTCCTT CATCAGCTGC 
ACCAAGTGGT TGGAGAAGGT CCGGTCTCAG ACTTCAGGCA TCTCCCTCCC AGGTGTTTTA 
GTGGGAACTA AGACAGACCT GGCTGGCCGA CAAACAGTGG ATTCAGCTCA GGCCCAGGTA 
TGGGCACTGA GTCAAGGCCT GGAATTCTTT GAGACATCTG TGAAGGAGAT GGATAATTAC 
GAGGCCCCGT TCCACTGTCT GGCCAAGCAG TTCTACCAGC TGTACCGGGA AAAAGTGGAC 
ATATTCCATA CCCTGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080207.1
CDS	307..867
Translation	MVKLAAKCILAGDPAVGKTALVQMFRSDGTHFQKNYTLTTGVDLVVKTVPVLDTNDSVELFIFDSAGKELFSEMLDKLWENPNVLCLVYDVTNEQSFISCTKWLEKVRSQTSGISLPGVLVGTKTDLAGRQTVDSAQAQVWALSQGLEFFETSVKEMDNYEAPFHCLAKQFYQLYREKVDIFHTLV

Target ORF information:

RefSeq Version	NM_025931.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus intraflagellar transport 27 (Ift27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025931.2

ORF Insert Sequence:

ATGGTGAAGC TAGCTGCCAA ATGCATCCTG GCAGGGGACC CAGCCGTAGG CAAGACGGCC 
CTGGTGCAGA TGTTCCGCAG CGACGGGACC CATTTCCAGA AGAACTACAC CCTGACAACT 
GGAGTGGATT TGGTGGTGAA GACAGTGCCA GTTCTTGACA CAAATGACAG TGTGGAACTC 
TTCATTTTTG ACTCAGCCGG CAAGGAGCTG TTCTCTGAAA TGCTGGATAA GTTGTGGGAG 
AATCCCAACG TCCTGTGCCT TGTCTATGAT GTGACCAATG AACAGTCCTT CATCAGCTGC 
ACCAAGTGGT TGGAGAAGGT CCGGTCTCAG ACTTCAGGCA TCTCCCTCCC AGGTGTTTTA 
GTGGGAACTA AGACAGACCT GGCTGGCCGA CAAACAGTGG ATTCAGCTCA GGCCCAGGTA 
TGGGCACTGA GTCAAGGCCT GGAATTCTTT GAGACATCTG TGAAGGAGAT GGATAATTAC 
GAGGCCCCGT TCCACTGTCT GGCCAAGCAG TTCTACCAGC TGTACCGGGA AAAAGTGGAC 
ATATTCCATA CCCTGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Intraflagellar transporter protein (IFT27), an IFT25 binding partner, is essential for male fertility and spermiogenesis in mice.
Developmental biology432(1)125-139(2017 Dec)
Zhang Y,Liu H,Li W,Zhang Z,Shang X,Zhang D,Li Y,Zhang S,Liu J,Hess RA,Pazour GJ,Zhang Z

IFT25, an intraflagellar transporter protein dispensable for ciliogenesis in somatic cells, is essential for sperm flagella formation.
Biology of reproduction96(5)993-1006(2017 May)
Liu H,Li W,Zhang Y,Zhang Z,Shang X,Zhang L,Zhang S,Li Y,Somoza AV,Delpi B,Gerton GL,Foster JA,Hess RA,Pazour GJ,Zhang Z

IFT56 regulates vertebrate developmental patterning by maintaining IFTB complex integrity and ciliary microtubule architecture.
Development (Cambridge, England)144(8)1544-1553(2017 04)
Xin D,Christopher KJ,Zeng L,Kong Y,Weatherbee SD

Cilia gene mutations cause atrioventricular septal defects by multiple mechanisms.
Human molecular genetics25(14)3011-3028(2016 07)
Burnicka-Turek O,Steimle JD,Huang W,Felker L,Kamp A,Kweon J,Peterson M,Reeves RH,Maslen CL,Gruber PJ,Yang XH,Shendure J,Moskowitz IP

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Ift27 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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