Med11 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Med11
Entrez Gene ID	66172
Full Name	mediator complex subunit 11
Synonyms	1110030J09Rik,AI465144,AW545069
General protein information	Preferred Names mediator complex subunit 11 Names mediator of RNA polymerase II transcription subunit 11 mediator of RNA polymerase II transcription, subunit 11 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_025397.2	NP_079673.2	mediator of RNA polymerase II transcription subunit 11
NM_025397.3	NP_079673.2	mediator of RNA polymerase II transcription subunit 11

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MED11	XP_849319.2
Bos taurus (cattle)	MED11	NP_001033173.1
Danio rerio (zebrafish)	LOC100534983	XP_003199394.1
	MED11	XP_001095324.1
Drosophila melanogaster (fruit fly)	MED11	NP_649057.1
Homo sapiens (human)	MED11	NP_001001683.1
Rattus norvegicus (Norway rat)	Med11	NP_001099269.1
Danio rerio (zebrafish)	med11	NP_001153507.1
Mus musculus (house mouse)	Med11	NP_079673.2
Pan troglodytes (chimpanzee)	MED11	XP_001161315.1
Xenopus tropicalis (tropical clawed frog)	med11	NP_989379.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.
Tsai KL, Tomomori-Sato C, Sato S, Conaway RC, Conaway JW, Asturias FJ
Cell157(6)1430-44(2014 Jun)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2.
Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens L, Zybailov B, Washburn MP, Brower CS, Conaway RC, Conaway JW
The Journal of biological chemistry279(7)5846-51(2004 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Med11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Med11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14551	NM_025397.2 Latest version!	Mus musculus mediator complex subunit 11 (Med11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu84581	NM_025397.3 Latest version!	Mus musculus mediator complex subunit 11 (Med11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Med11 ( NM_025397.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079673.2 Mus musculus mediator complex subunit 11 (Med11), mRNA.

Price & Availability↑

CloneID	OMu14551
Clone ID Related Accession (Same CDS sequence)	NM_025397.2
Accession Version	NM_025397.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 11
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC025057.1. On Jan 15, 2003 this sequence version replaced NM_025397.1. ##Evidence-Data-START## Transcript exon combination :: BC025057.1, DV064684.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCTACCT ACAGTCTGGC GAACGAGCGG CTGCGCGCCC TGGAGGATAT CGAGCGCGAA 
ATCGGCGCCA TCCTCCAGAA CGCAGGAACG GCGATCCTGG AACTGTCCAA GGAAAAGACT 
AACGAGCGGC TCCTCGACCG GCAGGCGGCA GCCTTCACCA CTTCTGTGCA GCACGTGGAG 
GCAGAGCTAT CCGCTCAAAT CCGCTACCTC ACTCAGGTGG CCACAGGGCA GCCGCATGAG 
GGCTCCAGCT ACTCTTCCAG GAAGGACTGT CAAATGGCTC TAAAGCGAGT AGACTACGCC 
CGCCTCAAGA TTAGTGATGT GGCGAGAACC TGTGAGCAGA TGCTGGAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079673.2
CDS	49..402
Translation	MATYSLANERLRALEDIEREIGAILQNAGTAILELSKEKTNERLLDRQAAAFTTSVQHVEAELSAQIRYLTQVATGQPHEGSSYSSRKDCQMALKRVDYARLKISDVARTCEQMLEN

Target ORF information:

RefSeq Version	NM_025397.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 11 (Med11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025397.2

ORF Insert Sequence:

ATGGCTACCT ACAGTCTGGC GAACGAGCGG CTGCGCGCCC TGGAGGATAT CGAGCGCGAA 
ATCGGCGCCA TCCTCCAGAA CGCAGGAACG GCGATCCTGG AACTGTCCAA GGAAAAGACT 
AACGAGCGGC TCCTCGACCG GCAGGCGGCA GCCTTCACCA CTTCTGTGCA GCACGTGGAG 
GCAGAGCTAT CCGCTCAAAT CCGCTACCTC ACTCAGGTGG CCACAGGGCA GCCGCATGAG 
GGCTCCAGCT ACTCTTCCAG GAAGGACTGT CAAATGGCTC TAAAGCGAGT AGACTACGCC 
CGCCTCAAGA TTAGTGATGT GGCGAGAACC TGTGAGCAGA TGCTGGAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med11 ( NM_025397.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079673.2 Mus musculus mediator complex subunit 11 (Med11), mRNA.

Price & Availability↑

CloneID	OMu84581
Clone ID Related Accession (Same CDS sequence)	NM_025397.3
Accession Version	NM_025397.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 11
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY171915.1, AK008153.1 and CF740526.1. On Jan 25, 2019 this sequence version replaced NM_025397.2. ##Evidence-Data-START## Transcript exon combination :: BC025057.1, AK003984.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCTACCT ACAGTCTGGC GAACGAGCGG CTGCGCGCCC TGGAGGATAT CGAGCGTGAA 
ATCGGCGCCA TCCTCCAGAA CGCAGGAACG GCGATCCTGG AACTGTCCAA GGAAAAGACT 
AACGAGCGGC TCCTCGACCG GCAGGCGGCA GCCTTCACCA CTTCTGTGCA GCACGTGGAG 
GCAGAGCTAT CCGCTCAAAT CCGCTACCTC ACTCAGGTGG CCACAGGGCA GCCGCATGAG 
GGCTCCAGCT ACTCTTCCAG GAAGGACTGT CAAATGGCTC TAAAGCGAGT AGACTACGCC 
CGCCTCAAGA TTAGTGATGT GGCGAGAACC TGTGAGCAGA TGCTGGAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079673.2
CDS	62..415
Translation	MATYSLANERLRALEDIEREIGAILQNAGTAILELSKEKTNERLLDRQAAAFTTSVQHVEAELSAQIRYLTQVATGQPHEGSSYSSRKDCQMALKRVDYARLKISDVARTCEQMLEN

Target ORF information:

RefSeq Version	NM_025397.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 11 (Med11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025397.3

ORF Insert Sequence:

ATGGCTACCT ACAGTCTGGC GAACGAGCGG CTGCGCGCCC TGGAGGATAT CGAGCGTGAA 
ATCGGCGCCA TCCTCCAGAA CGCAGGAACG GCGATCCTGG AACTGTCCAA GGAAAAGACT 
AACGAGCGGC TCCTCGACCG GCAGGCGGCA GCCTTCACCA CTTCTGTGCA GCACGTGGAG 
GCAGAGCTAT CCGCTCAAAT CCGCTACCTC ACTCAGGTGG CCACAGGGCA GCCGCATGAG 
GGCTCCAGCT ACTCTTCCAG GAAGGACTGT CAAATGGCTC TAAAGCGAGT AGACTACGCC 
CGCCTCAAGA TTAGTGATGT GGCGAGAACC TGTGAGCAGA TGCTGGAGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.
Cell157(6)1430-44(2014 Jun)
Tsai KL,Tomomori-Sato C,Sato S,Conaway RC,Conaway JW,Asturias FJ

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2.
The Journal of biological chemistry279(7)5846-51(2004 Feb)
Tomomori-Sato C,Sato S,Parmely TJ,Banks CA,Sorokina I,Florens L,Zybailov B,Washburn MP,Brower CS,Conaway RC,Conaway JW

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Med11 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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