Rhot1 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_011249167.2	XP_011247469.1	mitochondrial Rho GTPase 1 isoform X2
NM_001362868.1	NP_001349797.1	mitochondrial Rho GTPase 1 isoform 4
NM_021536.7	NP_067511.4	mitochondrial Rho GTPase 1 isoform 1
XM_006533886.4	XP_006533949.1	mitochondrial Rho GTPase 1 isoform X1
XM_011249167.3	XP_011247469.1	mitochondrial Rho GTPase 1 isoform X2
NM_021536.8	NP_067511.4	mitochondrial Rho GTPase 1 isoform 1
NM_001163355.2	NP_001156827.1	mitochondrial Rho GTPase 1 isoform 3
XM_011249168.2	XP_011247470.1	mitochondrial Rho GTPase 1 isoform X3
NM_001163355.1	NP_001156827.1	mitochondrial Rho GTPase 1 isoform 3
XM_006533886.3	XP_006533949.1	mitochondrial Rho GTPase 1 isoform X1
NM_001163354.1	NP_001156826.1	mitochondrial Rho GTPase 1 isoform 2
NM_001163354.2	NP_001156826.1	mitochondrial Rho GTPase 1 isoform 2
NM_001362870.1	NP_001349799.1	mitochondrial Rho GTPase 1 isoform 6
NM_001362869.1	NP_001349798.1	mitochondrial Rho GTPase 1 isoform 5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu08930	NM_001163354.2 Latest version!	Mus musculus ras homolog family member T1 (Rhot1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$391.30 $559.00
OMu12458	NM_021536.8 Latest version!	Mus musculus ras homolog family member T1 (Rhot1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$370.30 $529.00
OMu08933	NM_001163355.2 Latest version!	Mus musculus ras homolog family member T1 (Rhot1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu69819	NM_001362868.1 Latest version!	Mus musculus ras homolog family member T1 (Rhot1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu69819	XM_011249168.2 Latest version!	Mus musculus ras homolog family member T1 (Rhot1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu84384	NM_001362870.1 Latest version!	Mus musculus ras homolog family member T1 (Rhot1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu84395	NM_001362869.1 Latest version!	Mus musculus ras homolog family member T1 (Rhot1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu46724	XM_006533886.4 Latest version!	Mus musculus ras homolog family member T1 (Rhot1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu69818	XM_011249167.3 Latest version!	Mus musculus ras homolog family member T1 (Rhot1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu69818	XM_011249167.2	Mus musculus ras homolog family member T1 (Rhot1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu12458	NM_021536.7	Mus musculus ras homolog family member T1 (Rhot1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$370.30 $529.00
OMu08933	NM_001163355.1	Mus musculus ras homolog family member T1 (Rhot1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu46724	XM_006533886.3	Mus musculus ras homolog family member T1 (Rhot1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu08930	NM_001163354.1	Mus musculus ras homolog family member T1 (Rhot1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$391.30 $559.00
Hide Old Accession Versions >>

Rhot1 ( NM_001163354.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156826.1 Mus musculus ras homolog family member T1 (Rhot1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu08930
Clone ID Related Accession (Same CDS sequence)	NM_001163354.1 , NM_001163354.2
Accession Version	NM_001163354.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Mus musculus(house mouse)
Product	mitochondrial Rho GTPase 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147801.1 and AL591426.17. Transcript Variant: This variant (2) contains an additional in-frame exon in the 3' coding region, compared to variant 1, that results in a longer protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK147801.1, SRR1660817.251264.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCAG AGGATCATTA CAGAGGCAGC 
CTCTCCCGAG ACATGGGCAG CACTGATAGA ATAGAGAATT TGAGAAAAAT CTGGGTCTTT 
CTAAAAACTG CTTTGCACGT GACACAAGCT GACCTCAAGA GCTCAACGTT CTGGCTTCGC 
GCAAGCTTTG GTGCTACGGT CTTTGCAGTC GTGGGCTTTG CTATGTACAG AGCACTACTG 
AAACAGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156826.1
CDS	194..2185
Translation	MRAGRVRPLRASDMKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHLSDGVADSGLTLRGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFQVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAITVTRDKKIDLQKKQTQRNVFRCNVIGVKGCGKTGVLQSLLGRNLMRQKKIRDDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAETICDVVCLVYDVTNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEHSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPEDHYRGSLSRDMGSTDRIENLRKIWVFLKTALHVTQADLKSSTFWLRASFGATVFAVVGFAMYRALLKQR

Target ORF information:

RefSeq Version	NM_001163354.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member T1 (Rhot1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163354.1

ORF Insert Sequence:

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCAG AGGATCATTA CAGAGGCAGC 
CTCTCCCGAG ACATGGGCAG CACTGATAGA ATAGAGAATT TGAGAAAAAT CTGGGTCTTT 
CTAAAAACTG CTTTGCACGT GACACAAGCT GACCTCAAGA GCTCAACGTT CTGGCTTCGC 
GCAAGCTTTG GTGCTACGGT CTTTGCAGTC GTGGGCTTTG CTATGTACAG AGCACTACTG 
AAACAGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhot1 ( NM_001163354.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156826.1 Mus musculus ras homolog family member T1 (Rhot1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu08930
Clone ID Related Accession (Same CDS sequence)	NM_001163354.1 , NM_001163354.2
Accession Version	NM_001163354.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Mus musculus(house mouse)
Product	mitochondrial Rho GTPase 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147801.1 and AL591426.17. On May 2, 2018 this sequence version replaced NM_001163354.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147801.1, SRR1660817.251264.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCAG AGGATCATTA CAGAGGCAGC 
CTCTCCCGAG ACATGGGCAG CACTGATAGA ATAGAGAATT TGAGAAAAAT CTGGGTCTTT 
CTAAAAACTG CTTTGCACGT GACACAAGCT GACCTCAAGA GCTCAACGTT CTGGCTTCGC 
GCAAGCTTTG GTGCTACGGT CTTTGCAGTC GTGGGCTTTG CTATGTACAG AGCACTACTG 
AAACAGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156826.1
CDS	198..2189
Translation	MRAGRVRPLRASDMKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHLSDGVADSGLTLRGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFQVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAITVTRDKKIDLQKKQTQRNVFRCNVIGVKGCGKTGVLQSLLGRNLMRQKKIRDDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAETICDVVCLVYDVTNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEHSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPEDHYRGSLSRDMGSTDRIENLRKIWVFLKTALHVTQADLKSSTFWLRASFGATVFAVVGFAMYRALLKQR

Target ORF information:

RefSeq Version	NM_001163354.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member T1 (Rhot1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163354.2

ORF Insert Sequence:

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCAG AGGATCATTA CAGAGGCAGC 
CTCTCCCGAG ACATGGGCAG CACTGATAGA ATAGAGAATT TGAGAAAAAT CTGGGTCTTT 
CTAAAAACTG CTTTGCACGT GACACAAGCT GACCTCAAGA GCTCAACGTT CTGGCTTCGC 
GCAAGCTTTG GTGCTACGGT CTTTGCAGTC GTGGGCTTTG CTATGTACAG AGCACTACTG 
AAACAGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhot1 ( NM_021536.7 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067511.4 Mus musculus ras homolog family member T1 (Rhot1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu12458
Clone ID Related Accession (Same CDS sequence)	NM_021536.7 , NM_021536.8
Accession Version	NM_021536.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Mus musculus(house mouse)
Product	mitochondrial Rho GTPase 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147801.1, AK054472.1 and AL591426.17. On Jul 17, 2009 this sequence version replaced NM_021536.6. Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK138830.1, AK054472.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCGC ACGTGACACA AGCTGACCTC 
AAGAGCTCAA CGTTCTGGCT TCGCGCAAGC TTTGGTGCTA CGGTCTTTGC AGTCGTGGGC 
TTTGCTATGT ACAGAGCACT ACTGAAACAG CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067511.4
CDS	194..2089
Translation	MRAGRVRPLRASDMKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHLSDGVADSGLTLRGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFQVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAITVTRDKKIDLQKKQTQRNVFRCNVIGVKGCGKTGVLQSLLGRNLMRQKKIRDDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAETICDVVCLVYDVTNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEHSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPHVTQADLKSSTFWLRASFGATVFAVVGFAMYRALLKQR

Target ORF information:

RefSeq Version	NM_021536.7
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member T1 (Rhot1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021536.7

ORF Insert Sequence:

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCGC ACGTGACACA AGCTGACCTC 
AAGAGCTCAA CGTTCTGGCT TCGCGCAAGC TTTGGTGCTA CGGTCTTTGC AGTCGTGGGC 
TTTGCTATGT ACAGAGCACT ACTGAAACAG CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhot1 ( NM_021536.8 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067511.4 Mus musculus ras homolog family member T1 (Rhot1), transcript variant 1, mRNA.

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CloneID	OMu12458
Clone ID Related Accession (Same CDS sequence)	NM_021536.7 , NM_021536.8
Accession Version	NM_021536.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Mus musculus(house mouse)
Product	mitochondrial Rho GTPase 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147801.1, AK054472.1 and AL591426.17. On May 2, 2018 this sequence version replaced NM_021536.7. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK138830.1, AK054472.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCGC ACGTGACACA AGCTGACCTC 
AAGAGCTCAA CGTTCTGGCT TCGCGCAAGC TTTGGTGCTA CGGTCTTTGC AGTCGTGGGC 
TTTGCTATGT ACAGAGCACT ACTGAAACAG CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067511.4
CDS	198..2093
Translation	MRAGRVRPLRASDMKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHLSDGVADSGLTLRGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFQVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAITVTRDKKIDLQKKQTQRNVFRCNVIGVKGCGKTGVLQSLLGRNLMRQKKIRDDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAETICDVVCLVYDVTNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEHSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPHVTQADLKSSTFWLRASFGATVFAVVGFAMYRALLKQR

Target ORF information:

RefSeq Version	NM_021536.8
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member T1 (Rhot1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021536.8

ORF Insert Sequence:

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCGC ACGTGACACA AGCTGACCTC 
AAGAGCTCAA CGTTCTGGCT TCGCGCAAGC TTTGGTGCTA CGGTCTTTGC AGTCGTGGGC 
TTTGCTATGT ACAGAGCACT ACTGAAACAG CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhot1 ( NM_001163355.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156827.1 Mus musculus ras homolog family member T1 (Rhot1), transcript variant 3, mRNA.

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CloneID	OMu08933
Clone ID Related Accession (Same CDS sequence)	NM_001163355.2 , NM_001163355.1
Accession Version	NM_001163355.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Mus musculus(house mouse)
Product	mitochondrial Rho GTPase 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147801.1, AK167502.1 and AL591426.17. On May 2, 2018 this sequence version replaced NM_001163355.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK141621.1, AK167502.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCCC ATGCCCGGTT ACGCTGCATG 
TGCACCTGCA ACAGGTGTAC ATTTTGCATC TGTCAGAACT TCCTCAACTC AGACTTGCTG 
CAATCTGTAA AGAACAAAAT CTTCACTGCA GTTCTTAACA GGCACGTGAC ACAAGCTGAC 
CTCAAGAGCT CAACGTTCTG GCTTCGCGCA AGCTTTGGTG CTACGGTCTT TGCAGTCGTG 
GGCTTTGCTA TGTACAGAGC ACTACTGAAA CAGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156827.1
CDS	198..2216
Translation	MRAGRVRPLRASDMKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHLSDGVADSGLTLRGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFQVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAITVTRDKKIDLQKKQTQRNVFRCNVIGVKGCGKTGVLQSLLGRNLMRQKKIRDDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAETICDVVCLVYDVTNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEHSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPHARLRCMCTCNRCTFCICQNFLNSDLLQSVKNKIFTAVLNRHVTQADLKSSTFWLRASFGATVFAVVGFAMYRALLKQR

Target ORF information:

RefSeq Version	NM_001163355.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member T1 (Rhot1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163355.2

ORF Insert Sequence:

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCCC ATGCCCGGTT ACGCTGCATG 
TGCACCTGCA ACAGGTGTAC ATTTTGCATC TGTCAGAACT TCCTCAACTC AGACTTGCTG 
CAATCTGTAA AGAACAAAAT CTTCACTGCA GTTCTTAACA GGCACGTGAC ACAAGCTGAC 
CTCAAGAGCT CAACGTTCTG GCTTCGCGCA AGCTTTGGTG CTACGGTCTT TGCAGTCGTG 
GGCTTTGCTA TGTACAGAGC ACTACTGAAA CAGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhot1 ( NM_001163355.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156827.1 Mus musculus ras homolog family member T1 (Rhot1), transcript variant 3, mRNA.

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CloneID	OMu08933
Clone ID Related Accession (Same CDS sequence)	NM_001163355.2 , NM_001163355.1
Accession Version	NM_001163355.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Mus musculus(house mouse)
Product	mitochondrial Rho GTPase 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK167502.1 and AL591426.17. Transcript Variant: This variant (3) contains an additional in-frame exon in the 3' coding region, compared to variant 1, that results in a longer protein (isoform 3), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK167502.1, SRR1660823.31561.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCCC ATGCCCGGTT ACGCTGCATG 
TGCACCTGCA ACAGGTGTAC ATTTTGCATC TGTCAGAACT TCCTCAACTC AGACTTGCTG 
CAATCTGTAA AGAACAAAAT CTTCACTGCA GTTCTTAACA GGCACGTGAC ACAAGCTGAC 
CTCAAGAGCT CAACGTTCTG GCTTCGCGCA AGCTTTGGTG CTACGGTCTT TGCAGTCGTG 
GGCTTTGCTA TGTACAGAGC ACTACTGAAA CAGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156827.1
CDS	194..2212
Translation	MRAGRVRPLRASDMKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHLSDGVADSGLTLRGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFQVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAITVTRDKKIDLQKKQTQRNVFRCNVIGVKGCGKTGVLQSLLGRNLMRQKKIRDDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAETICDVVCLVYDVTNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEHSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPHARLRCMCTCNRCTFCICQNFLNSDLLQSVKNKIFTAVLNRHVTQADLKSSTFWLRASFGATVFAVVGFAMYRALLKQR

Target ORF information:

RefSeq Version	NM_001163355.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member T1 (Rhot1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163355.1

ORF Insert Sequence:

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCCC ATGCCCGGTT ACGCTGCATG 
TGCACCTGCA ACAGGTGTAC ATTTTGCATC TGTCAGAACT TCCTCAACTC AGACTTGCTG 
CAATCTGTAA AGAACAAAAT CTTCACTGCA GTTCTTAACA GGCACGTGAC ACAAGCTGAC 
CTCAAGAGCT CAACGTTCTG GCTTCGCGCA AGCTTTGGTG CTACGGTCTT TGCAGTCGTG 
GGCTTTGCTA TGTACAGAGC ACTACTGAAA CAGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhot1 ( NM_001362868.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001349797.1 Mus musculus ras homolog family member T1 (Rhot1), transcript variant 4, mRNA.

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CloneID	OMu69819
Clone ID Related Accession (Same CDS sequence)	NM_001362868.1 , XM_011249168.2
Accession Version	NM_001362868.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Mus musculus(house mouse)
Product	mitochondrial Rho GTPase 1 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591426.17. On May 2, 2018 this sequence version replaced XM_011249168.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.68438.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCAG AGGATCATTA CAGAGGCAGC 
CTCTCCCGAG ACATGGGCAG CACTGATAGA ATAGAGAATT TGAGAAAAAT CTGGGTCTTT 
CTAAAAACTG CTTTCCATGC CCGGTTACGC TGCATGTGCA CCTGCAACAG GTGTACATTT 
TGCATCTGTC AGAACTTCCT CAACTCAGAC TTGCTGCAAT CTGTAAAGAA CAAAATCTTC 
ACTGCAGTTC TTAACAGGCA CGTGACACAA GCTGACCTCA AGAGCTCAAC GTTCTGGCTT 
CGCGCAAGCT TTGGTGCTAC GGTCTTTGCA GTCGTGGGCT TTGCTATGTA CAGAGCACTA 
CTGAAACAGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349797.1
CDS	198..2312
Translation	MRAGRVRPLRASDMKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHLSDGVADSGLTLRGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFQVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAITVTRDKKIDLQKKQTQRNVFRCNVIGVKGCGKTGVLQSLLGRNLMRQKKIRDDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAETICDVVCLVYDVTNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEHSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPEDHYRGSLSRDMGSTDRIENLRKIWVFLKTAFHARLRCMCTCNRCTFCICQNFLNSDLLQSVKNKIFTAVLNRHVTQADLKSSTFWLRASFGATVFAVVGFAMYRALLKQR

Target ORF information:

RefSeq Version	NM_001362868.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member T1 (Rhot1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362868.1

ORF Insert Sequence:

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCAG AGGATCATTA CAGAGGCAGC 
CTCTCCCGAG ACATGGGCAG CACTGATAGA ATAGAGAATT TGAGAAAAAT CTGGGTCTTT 
CTAAAAACTG CTTTCCATGC CCGGTTACGC TGCATGTGCA CCTGCAACAG GTGTACATTT 
TGCATCTGTC AGAACTTCCT CAACTCAGAC TTGCTGCAAT CTGTAAAGAA CAAAATCTTC 
ACTGCAGTTC TTAACAGGCA CGTGACACAA GCTGACCTCA AGAGCTCAAC GTTCTGGCTT 
CGCGCAAGCT TTGGTGCTAC GGTCTTTGCA GTCGTGGGCT TTGCTATGTA CAGAGCACTA 
CTGAAACAGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhot1 ( XM_006533886.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533949.1 Mus musculus ras homolog family member T1 (Rhot1), transcript variant X1, mRNA.

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CloneID	OMu46724
Clone ID Related Accession (Same CDS sequence)	XM_006533886.4 , XM_006533886.3
Accession Version	XM_006533886.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mitochondrial Rho GTPase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533886.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCAG AGGATCATTA CAGAGGCAGC 
CTCTCCCGAG ACATGGGCAG CACTGATAGA ATAGAGAATT TGAGAAAAAT CTGGGTCTTT 
CTAAAAACTG CTTTCCATGC CCGGTTACGC TGCATGTGCA CCTGCAACAG GTGTACATTT 
TGCATCTGTC AGAACTTCCT CAACTCAGAC TTGCTGCAAT CTGTAAAGAA CAAAATCTTC 
ACTGCAGTTC TTAACAGGTT AAAAGCCCGG GTAGCTGAGT GGGGTGCCAT GCTGTTAGCA 
GACGACGAAG GCAGTATTAC TCACCAGGTG CACGCCGTCA GCTCCGTAGA AGATTCCATC 
TTCATGGAGT CATCTCACGG TCAGCTTTTT CTTGATGACA GGCACGTGAC ACAAGCTGAC 
CTCAAGAGCT CAACGTTCTG GCTTCGCGCA AGCTTTGGTG CTACGGTCTT TGCAGTCGTG 
GGCTTTGCTA TGTACAGAGC ACTACTGAAA CAGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533949.1
CDS	217..2475
Translation	MRAGRVRPLRASDMKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHLSDGVADSGLTLRGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFQVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAITVTRDKKIDLQKKQTQRNVFRCNVIGVKGCGKTGVLQSLLGRNLMRQKKIRDDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAETICDVVCLVYDVTNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEHSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPEDHYRGSLSRDMGSTDRIENLRKIWVFLKTAFHARLRCMCTCNRCTFCICQNFLNSDLLQSVKNKIFTAVLNRLKARVAEWGAMLLADDEGSITHQVHAVSSVEDSIFMESSHGQLFLDDRHVTQADLKSSTFWLRASFGATVFAVVGFAMYRALLKQR

Target ORF information:

RefSeq Version	XM_006533886.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member T1 (Rhot1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533886.3

ORF Insert Sequence:

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCAG AGGATCATTA CAGAGGCAGC 
CTCTCCCGAG ACATGGGCAG CACTGATAGA ATAGAGAATT TGAGAAAAAT CTGGGTCTTT 
CTAAAAACTG CTTTCCATGC CCGGTTACGC TGCATGTGCA CCTGCAACAG GTGTACATTT 
TGCATCTGTC AGAACTTCCT CAACTCAGAC TTGCTGCAAT CTGTAAAGAA CAAAATCTTC 
ACTGCAGTTC TTAACAGGTT AAAAGCCCGG GTAGCTGAGT GGGGTGCCAT GCTGTTAGCA 
GACGACGAAG GCAGTATTAC TCACCAGGTG CACGCCGTCA GCTCCGTAGA AGATTCCATC 
TTCATGGAGT CATCTCACGG TCAGCTTTTT CTTGATGACA GGCACGTGAC ACAAGCTGAC 
CTCAAGAGCT CAACGTTCTG GCTTCGCGCA AGCTTTGGTG CTACGGTCTT TGCAGTCGTG 
GGCTTTGCTA TGTACAGAGC ACTACTGAAA CAGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhot1 ( XM_011249167.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247469.1 Mus musculus ras homolog family member T1 (Rhot1), transcript variant X2, mRNA.

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CloneID	OMu69818
Clone ID Related Accession (Same CDS sequence)	XM_011249167.2 , XM_011249167.3
Accession Version	XM_011249167.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mitochondrial Rho GTPase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249167.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCCC ATGCCCGGTT ACGCTGCATG 
TGCACCTGCA ACAGGTGTAC ATTTTGCATC TGTCAGAACT TCCTCAACTC AGACTTGCTG 
CAATCTGTAA AGAACAAAAT CTTCACTGCA GTTCTTAACA GGTTAAAAGC CCGGGTAGCT 
GAGTGGGGTG CCATGCTGTT AGCAGACGAC GAAGGCAGTA TTACTCACCA GGTGCACGCC 
GTCAGCTCCG TAGAAGATTC CATCTTCATG GAGTCATCTC ACGGTCAGCT TTTTCTTGAT 
GACAGGCACG TGACACAAGC TGACCTCAAG AGCTCAACGT TCTGGCTTCG CGCAAGCTTT 
GGTGCTACGG TCTTTGCAGT CGTGGGCTTT GCTATGTACA GAGCACTACT GAAACAGCGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247469.1
CDS	216..2378
Translation	MRAGRVRPLRASDMKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHLSDGVADSGLTLRGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFQVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAITVTRDKKIDLQKKQTQRNVFRCNVIGVKGCGKTGVLQSLLGRNLMRQKKIRDDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAETICDVVCLVYDVTNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEHSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPHARLRCMCTCNRCTFCICQNFLNSDLLQSVKNKIFTAVLNRLKARVAEWGAMLLADDEGSITHQVHAVSSVEDSIFMESSHGQLFLDDRHVTQADLKSSTFWLRASFGATVFAVVGFAMYRALLKQR

Target ORF information:

RefSeq Version	XM_011249167.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member T1 (Rhot1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249167.2

ORF Insert Sequence:

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCCC ATGCCCGGTT ACGCTGCATG 
TGCACCTGCA ACAGGTGTAC ATTTTGCATC TGTCAGAACT TCCTCAACTC AGACTTGCTG 
CAATCTGTAA AGAACAAAAT CTTCACTGCA GTTCTTAACA GGTTAAAAGC CCGGGTAGCT 
GAGTGGGGTG CCATGCTGTT AGCAGACGAC GAAGGCAGTA TTACTCACCA GGTGCACGCC 
GTCAGCTCCG TAGAAGATTC CATCTTCATG GAGTCATCTC ACGGTCAGCT TTTTCTTGAT 
GACAGGCACG TGACACAAGC TGACCTCAAG AGCTCAACGT TCTGGCTTCG CGCAAGCTTT 
GGTGCTACGG TCTTTGCAGT CGTGGGCTTT GCTATGTACA GAGCACTACT GAAACAGCGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhot1 ( XM_011249168.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247470.1 Mus musculus ras homolog family member T1 (Rhot1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu69819
Clone ID Related Accession (Same CDS sequence)	NM_001362868.1 , XM_011249168.2
Accession Version	XM_011249168.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mitochondrial Rho GTPase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249168.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCAG AGGATCATTA CAGAGGCAGC 
CTCTCCCGAG ACATGGGCAG CACTGATAGA ATAGAGAATT TGAGAAAAAT CTGGGTCTTT 
CTAAAAACTG CTTTCCATGC CCGGTTACGC TGCATGTGCA CCTGCAACAG GTGTACATTT 
TGCATCTGTC AGAACTTCCT CAACTCAGAC TTGCTGCAAT CTGTAAAGAA CAAAATCTTC 
ACTGCAGTTC TTAACAGGCA CGTGACACAA GCTGACCTCA AGAGCTCAAC GTTCTGGCTT 
CGCGCAAGCT TTGGTGCTAC GGTCTTTGCA GTCGTGGGCT TTGCTATGTA CAGAGCACTA 
CTGAAACAGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247470.1
CDS	216..2330
Translation	MRAGRVRPLRASDMKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHLSDGVADSGLTLRGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFQVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAITVTRDKKIDLQKKQTQRNVFRCNVIGVKGCGKTGVLQSLLGRNLMRQKKIRDDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAETICDVVCLVYDVTNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEHSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPEDHYRGSLSRDMGSTDRIENLRKIWVFLKTAFHARLRCMCTCNRCTFCICQNFLNSDLLQSVKNKIFTAVLNRHVTQADLKSSTFWLRASFGATVFAVVGFAMYRALLKQR

Target ORF information:

RefSeq Version	XM_011249168.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member T1 (Rhot1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249168.2

ORF Insert Sequence:

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCAG AGGATCATTA CAGAGGCAGC 
CTCTCCCGAG ACATGGGCAG CACTGATAGA ATAGAGAATT TGAGAAAAAT CTGGGTCTTT 
CTAAAAACTG CTTTCCATGC CCGGTTACGC TGCATGTGCA CCTGCAACAG GTGTACATTT 
TGCATCTGTC AGAACTTCCT CAACTCAGAC TTGCTGCAAT CTGTAAAGAA CAAAATCTTC 
ACTGCAGTTC TTAACAGGCA CGTGACACAA GCTGACCTCA AGAGCTCAAC GTTCTGGCTT 
CGCGCAAGCT TTGGTGCTAC GGTCTTTGCA GTCGTGGGCT TTGCTATGTA CAGAGCACTA 
CTGAAACAGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhot1 ( NM_001362870.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001349799.1 Mus musculus ras homolog family member T1 (Rhot1), transcript variant 6, mRNA.

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CloneID	OMu84384
Clone ID Related Accession (Same CDS sequence)	NM_001362870.1
Accession Version	NM_001362870.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Mus musculus(house mouse)
Product	mitochondrial Rho GTPase 1 isoform 6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591426.17. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.22972.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCCC ATGCCCGGTT ACGCTGCATG 
TGCACCTGCA ACAGGTGTAC ATTTTGCATC TGTCAGAACT TCCTCAACTC AGACTTGCTG 
CAATCTGTAA AGAACAAAAT CTTCACTGCA GTTCTTAACA GGCACGTGAC ACAAGCTGAC 
CTCAAGAGCT CAACGTTCTG GCTTCGCGCA AGCTTTGGTG CTACGGTCTT TGCAGTCGTG 
GGCTTTGCTA TGTACAGAGC ACTACTGAAA CAGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349799.1
CDS	536..2134
Translation	METILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHLSDGVADSGLTLRGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFQVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAITVTRDKKIDLQKKQTQRNVFRCNVIGVKGCGKTGVLQSLLGRNLMRQKKIRDDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAETICDVVCLVYDVTNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEHSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPHARLRCMCTCNRCTFCICQNFLNSDLLQSVKNKIFTAVLNRHVTQADLKSSTFWLRASFGATVFAVVGFAMYRALLKQR

Target ORF information:

RefSeq Version	NM_001362870.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member T1 (Rhot1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362870.1

ORF Insert Sequence:

ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCCC ATGCCCGGTT ACGCTGCATG 
TGCACCTGCA ACAGGTGTAC ATTTTGCATC TGTCAGAACT TCCTCAACTC AGACTTGCTG 
CAATCTGTAA AGAACAAAAT CTTCACTGCA GTTCTTAACA GGCACGTGAC ACAAGCTGAC 
CTCAAGAGCT CAACGTTCTG GCTTCGCGCA AGCTTTGGTG CTACGGTCTT TGCAGTCGTG 
GGCTTTGCTA TGTACAGAGC ACTACTGAAA CAGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhot1 ( NM_001362869.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001349798.1 Mus musculus ras homolog family member T1 (Rhot1), transcript variant 5, mRNA.

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CloneID	OMu84395
Clone ID Related Accession (Same CDS sequence)	NM_001362869.1
Accession Version	NM_001362869.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Mus musculus(house mouse)
Product	mitochondrial Rho GTPase 1 isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591426.17. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.233813.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01164134 [ECO:0000350] ##Evidence-Data-END##

ATGAAAGAAC AGACAAAGAC AGCAGGATCT CACTATGTAT TCTTGGCTGG CTTGGAACTC 
ACAAAACTCC ACCTGCCCCA AAGAGCCGAG TGCTGGGATT TAAAGGAGCT GCCTTTGATA 
TTGGTTGGGA ACAAATCTGA TCTGGTGGAA TATAGTAGTA TGGAGACCAT CCTTCCAATT 
ATGAACCAGT ATACAGAGAT AGAGACCTGT GTGGAGTGTT CAGCAAAAAA CCTAAAGAAT 
ATCTCAGAGC TGTTTTATTA TGCACAGAAA GCTGTTCTTC ATCCTACAGG GCCTCTATAC 
TGCCCAGAGG AGAAAGAGAT GAAACCGGCT TGTATAAAGG CCCTCACTCG TATATTTAAA 
ATATCTGATC AGGATAACGA CGGCACTCTG AATGATGCTG AGCTCAACTT CTTCCAGAGA 
ATTTGTTTCA ACACTCCTTT AGCTCCGCAA GCGCTGGAAG ATGTCAAGAA TGTGGTTAGA 
AAGCACTTAA GTGACGGTGT GGCTGACAGT GGGCTGACGC TCAGAGGTTT TCTCTTTTTA 
CATACACTTT TTATCCAGAG GGGGAGGCAT GAGACTACTT GGACTGTGCT TCGACGATTT 
GGTTATGACG ATGACCTGGA CCTGACGCCT GAGTATTTAT TCCCCCTGCT AAAAATACCT 
CCTGATTGCA CTACTGAATT AAATCATCAT GCATATTTGT TTCTCCAGAG CACCTTTGAC 
AAACATGATT TGGACAGAGA CTGTGCTTTG TCGCCTGATG AACTTAAAGA TTTATTTCAA 
GTTTTTCCTT ACATACCTTG GGGACCAGAT GTAAATAACA CAGTTTGCAC AAATGAAAGA 
GGCTGGATAA CCTACCAGGG GTTCCTCTCC CAGTGGACGC TCACCACCTA CTTAGATGTG 
CAGCGTTGCC TGGAGTATTT GGGATATCTA GGCTATTCCA TACTGACGGA ACAAGAGTCT 
CAAGCTTCAG CCATTACAGT AACAAGGGAT AAAAAGATTG ACCTGCAGAA AAAACAGACT 
CAGAGAAATG TGTTCAGGTG TAATGTAATT GGAGTGAAAG GCTGTGGGAA AACTGGAGTT 
CTTCAGTCTC TCCTTGGAAG AAACCTAATG AGGCAGAAGA AAATTCGTGA TGATCATAAG 
TCCTATTATG CAATTAACAC TGTCTATGTA TATGGACAAG AGAAATACTT GTTGCTGCAC 
GATATCTCAG AGTCGGAATT TCTAACTGAG GCTGAGACCA TTTGTGATGT TGTATGCCTG 
GTATATGATG TCACCAATCC CAAATCCTTT GAATACTGTG CCAGGATTTT TAAGCAACAT 
TTTATGGACA GCAGAATTCC CTGCTTGATT GTAGCTGCAA AGTCAGACCT GCATGAAGTC 
AAACAGGAAC ATAGCATCTC ACCTACTGAT TTCTGCAGGA AACACAAAAT GCCTCCACCT 
CAAGCCTTCA CTTGCAATAC TGCTGATGCC CCCAGTAAGG ATATCTTTGT TAAGTTGACA 
ACAATGGCCA TGTACCCAGA GGATCATTAC AGAGGCAGCC TCTCCCGAGA CATGGGCAGC 
ACTGATAGAA TAGAGAATTT GAGAAAAATC TGGGTCTTTC TAAAAACTGC TTTGCACGTG 
ACACAAGCTG ACCTCAAGAG CTCAACGTTC TGGCTTCGCG CAAGCTTTGG TGCTACGGTC 
TTTGCAGTCG TGGGCTTTGC TATGTACAGA GCACTACTGA AACAGCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349798.1
CDS	516..2246
Translation	MKEQTKTAGSHYVFLAGLELTKLHLPQRAECWDLKELPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHLSDGVADSGLTLRGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFQVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAITVTRDKKIDLQKKQTQRNVFRCNVIGVKGCGKTGVLQSLLGRNLMRQKKIRDDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAETICDVVCLVYDVTNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEHSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPEDHYRGSLSRDMGSTDRIENLRKIWVFLKTALHVTQADLKSSTFWLRASFGATVFAVVGFAMYRALLKQR

Target ORF information:

RefSeq Version	NM_001362869.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member T1 (Rhot1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362869.1

ORF Insert Sequence:

ATGAAAGAAC AGACAAAGAC AGCAGGATCT CACTATGTAT TCTTGGCTGG CTTGGAACTC 
ACAAAACTCC ACCTGCCCCA AAGAGCCGAG TGCTGGGATT TAAAGGAGCT GCCTTTGATA 
TTGGTTGGGA ACAAATCTGA TCTGGTGGAA TATAGTAGTA TGGAGACCAT CCTTCCAATT 
ATGAACCAGT ATACAGAGAT AGAGACCTGT GTGGAGTGTT CAGCAAAAAA CCTAAAGAAT 
ATCTCAGAGC TGTTTTATTA TGCACAGAAA GCTGTTCTTC ATCCTACAGG GCCTCTATAC 
TGCCCAGAGG AGAAAGAGAT GAAACCGGCT TGTATAAAGG CCCTCACTCG TATATTTAAA 
ATATCTGATC AGGATAACGA CGGCACTCTG AATGATGCTG AGCTCAACTT CTTCCAGAGA 
ATTTGTTTCA ACACTCCTTT AGCTCCGCAA GCGCTGGAAG ATGTCAAGAA TGTGGTTAGA 
AAGCACTTAA GTGACGGTGT GGCTGACAGT GGGCTGACGC TCAGAGGTTT TCTCTTTTTA 
CATACACTTT TTATCCAGAG GGGGAGGCAT GAGACTACTT GGACTGTGCT TCGACGATTT 
GGTTATGACG ATGACCTGGA CCTGACGCCT GAGTATTTAT TCCCCCTGCT AAAAATACCT 
CCTGATTGCA CTACTGAATT AAATCATCAT GCATATTTGT TTCTCCAGAG CACCTTTGAC 
AAACATGATT TGGACAGAGA CTGTGCTTTG TCGCCTGATG AACTTAAAGA TTTATTTCAA 
GTTTTTCCTT ACATACCTTG GGGACCAGAT GTAAATAACA CAGTTTGCAC AAATGAAAGA 
GGCTGGATAA CCTACCAGGG GTTCCTCTCC CAGTGGACGC TCACCACCTA CTTAGATGTG 
CAGCGTTGCC TGGAGTATTT GGGATATCTA GGCTATTCCA TACTGACGGA ACAAGAGTCT 
CAAGCTTCAG CCATTACAGT AACAAGGGAT AAAAAGATTG ACCTGCAGAA AAAACAGACT 
CAGAGAAATG TGTTCAGGTG TAATGTAATT GGAGTGAAAG GCTGTGGGAA AACTGGAGTT 
CTTCAGTCTC TCCTTGGAAG AAACCTAATG AGGCAGAAGA AAATTCGTGA TGATCATAAG 
TCCTATTATG CAATTAACAC TGTCTATGTA TATGGACAAG AGAAATACTT GTTGCTGCAC 
GATATCTCAG AGTCGGAATT TCTAACTGAG GCTGAGACCA TTTGTGATGT TGTATGCCTG 
GTATATGATG TCACCAATCC CAAATCCTTT GAATACTGTG CCAGGATTTT TAAGCAACAT 
TTTATGGACA GCAGAATTCC CTGCTTGATT GTAGCTGCAA AGTCAGACCT GCATGAAGTC 
AAACAGGAAC ATAGCATCTC ACCTACTGAT TTCTGCAGGA AACACAAAAT GCCTCCACCT 
CAAGCCTTCA CTTGCAATAC TGCTGATGCC CCCAGTAAGG ATATCTTTGT TAAGTTGACA 
ACAATGGCCA TGTACCCAGA GGATCATTAC AGAGGCAGCC TCTCCCGAGA CATGGGCAGC 
ACTGATAGAA TAGAGAATTT GAGAAAAATC TGGGTCTTTC TAAAAACTGC TTTGCACGTG 
ACACAAGCTG ACCTCAAGAG CTCAACGTTC TGGCTTCGCG CAAGCTTTGG TGCTACGGTC 
TTTGCAGTCG TGGGCTTTGC TATGTACAGA GCACTACTGA AACAGCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhot1 ( XM_006533886.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533949.1 Mus musculus ras homolog family member T1 (Rhot1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu46724
Clone ID Related Accession (Same CDS sequence)	XM_006533886.4 , XM_006533886.3
Accession Version	XM_006533886.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mitochondrial Rho GTPase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533886.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCAG AGGATCATTA CAGAGGCAGC 
CTCTCCCGAG ACATGGGCAG CACTGATAGA ATAGAGAATT TGAGAAAAAT CTGGGTCTTT 
CTAAAAACTG CTTTCCATGC CCGGTTACGC TGCATGTGCA CCTGCAACAG GTGTACATTT 
TGCATCTGTC AGAACTTCCT CAACTCAGAC TTGCTGCAAT CTGTAAAGAA CAAAATCTTC 
ACTGCAGTTC TTAACAGGTT AAAAGCCCGG GTAGCTGAGT GGGGTGCCAT GCTGTTAGCA 
GACGACGAAG GCAGTATTAC TCACCAGGTG CACGCCGTCA GCTCCGTAGA AGATTCCATC 
TTCATGGAGT CATCTCACGG TCAGCTTTTT CTTGATGACA GGCACGTGAC ACAAGCTGAC 
CTCAAGAGCT CAACGTTCTG GCTTCGCGCA AGCTTTGGTG CTACGGTCTT TGCAGTCGTG 
GGCTTTGCTA TGTACAGAGC ACTACTGAAA CAGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533949.1
CDS	639..2897
Translation	MRAGRVRPLRASDMKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHLSDGVADSGLTLRGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFQVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAITVTRDKKIDLQKKQTQRNVFRCNVIGVKGCGKTGVLQSLLGRNLMRQKKIRDDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAETICDVVCLVYDVTNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEHSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPEDHYRGSLSRDMGSTDRIENLRKIWVFLKTAFHARLRCMCTCNRCTFCICQNFLNSDLLQSVKNKIFTAVLNRLKARVAEWGAMLLADDEGSITHQVHAVSSVEDSIFMESSHGQLFLDDRHVTQADLKSSTFWLRASFGATVFAVVGFAMYRALLKQR

Target ORF information:

RefSeq Version	XM_006533886.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member T1 (Rhot1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533886.4

ORF Insert Sequence:

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCAG AGGATCATTA CAGAGGCAGC 
CTCTCCCGAG ACATGGGCAG CACTGATAGA ATAGAGAATT TGAGAAAAAT CTGGGTCTTT 
CTAAAAACTG CTTTCCATGC CCGGTTACGC TGCATGTGCA CCTGCAACAG GTGTACATTT 
TGCATCTGTC AGAACTTCCT CAACTCAGAC TTGCTGCAAT CTGTAAAGAA CAAAATCTTC 
ACTGCAGTTC TTAACAGGTT AAAAGCCCGG GTAGCTGAGT GGGGTGCCAT GCTGTTAGCA 
GACGACGAAG GCAGTATTAC TCACCAGGTG CACGCCGTCA GCTCCGTAGA AGATTCCATC 
TTCATGGAGT CATCTCACGG TCAGCTTTTT CTTGATGACA GGCACGTGAC ACAAGCTGAC 
CTCAAGAGCT CAACGTTCTG GCTTCGCGCA AGCTTTGGTG CTACGGTCTT TGCAGTCGTG 
GGCTTTGCTA TGTACAGAGC ACTACTGAAA CAGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhot1 ( XM_011249167.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247469.1 Mus musculus ras homolog family member T1 (Rhot1), transcript variant X2, mRNA.

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CloneID	OMu69818
Clone ID Related Accession (Same CDS sequence)	XM_011249167.2 , XM_011249167.3
Accession Version	XM_011249167.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mitochondrial Rho GTPase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011249167.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCCC ATGCCCGGTT ACGCTGCATG 
TGCACCTGCA ACAGGTGTAC ATTTTGCATC TGTCAGAACT TCCTCAACTC AGACTTGCTG 
CAATCTGTAA AGAACAAAAT CTTCACTGCA GTTCTTAACA GGTTAAAAGC CCGGGTAGCT 
GAGTGGGGTG CCATGCTGTT AGCAGACGAC GAAGGCAGTA TTACTCACCA GGTGCACGCC 
GTCAGCTCCG TAGAAGATTC CATCTTCATG GAGTCATCTC ACGGTCAGCT TTTTCTTGAT 
GACAGGCACG TGACACAAGC TGACCTCAAG AGCTCAACGT TCTGGCTTCG CGCAAGCTTT 
GGTGCTACGG TCTTTGCAGT CGTGGGCTTT GCTATGTACA GAGCACTACT GAAACAGCGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247469.1
CDS	639..2801
Translation	MRAGRVRPLRASDMKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHLSDGVADSGLTLRGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFQVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAITVTRDKKIDLQKKQTQRNVFRCNVIGVKGCGKTGVLQSLLGRNLMRQKKIRDDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAETICDVVCLVYDVTNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEHSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPHARLRCMCTCNRCTFCICQNFLNSDLLQSVKNKIFTAVLNRLKARVAEWGAMLLADDEGSITHQVHAVSSVEDSIFMESSHGQLFLDDRHVTQADLKSSTFWLRASFGATVFAVVGFAMYRALLKQR

Target ORF information:

RefSeq Version	XM_011249167.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member T1 (Rhot1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249167.3

ORF Insert Sequence:

ATGCGTGCGG GCCGAGTGCG GCCCCTGCGA GCCTCCGACA TGAAGAAAGA CGTGAGGATC 
CTGCTGGTGG GAGAACCTCG AGTTGGGAAG ACATCACTGA TTATGTCGCT GGTCAGTGAA 
GAATTTCCGG AAGAGGTTCC TCCCCGAGCA GAAGAAATCA CCATTCCAGC TGACGTCACC 
CCTGAAAGAG TTCCAACACA CATTGTCGAT TATTCAGAAG CAGAACAGAG TGATGAACAA 
CTTCATCAAG AAATATCACA GGCTAATGTT ATCTGCATAG TTTATGCTGT TAACAACAAA 
CATTCTATTG ATAAGGTAAC AAGCCGATGG ATTCCTCTCA TAAATGAAAG AACAGACAAA 
GACAGCAGGC TGCCTTTGAT ATTGGTTGGG AACAAATCTG ATCTGGTGGA ATATAGTAGT 
ATGGAGACCA TCCTTCCAAT TATGAACCAG TATACAGAGA TAGAGACCTG TGTGGAGTGT 
TCAGCAAAAA ACCTAAAGAA TATCTCAGAG CTGTTTTATT ATGCACAGAA AGCTGTTCTT 
CATCCTACAG GGCCTCTATA CTGCCCAGAG GAGAAAGAGA TGAAACCGGC TTGTATAAAG 
GCCCTCACTC GTATATTTAA AATATCTGAT CAGGATAACG ACGGCACTCT GAATGATGCT 
GAGCTCAACT TCTTCCAGAG AATTTGTTTC AACACTCCTT TAGCTCCGCA AGCGCTGGAA 
GATGTCAAGA ATGTGGTTAG AAAGCACTTA AGTGACGGTG TGGCTGACAG TGGGCTGACG 
CTCAGAGGTT TTCTCTTTTT ACATACACTT TTTATCCAGA GGGGGAGGCA TGAGACTACT 
TGGACTGTGC TTCGACGATT TGGTTATGAC GATGACCTGG ACCTGACGCC TGAGTATTTA 
TTCCCCCTGC TAAAAATACC TCCTGATTGC ACTACTGAAT TAAATCATCA TGCATATTTG 
TTTCTCCAGA GCACCTTTGA CAAACATGAT TTGGACAGAG ACTGTGCTTT GTCGCCTGAT 
GAACTTAAAG ATTTATTTCA AGTTTTTCCT TACATACCTT GGGGACCAGA TGTAAATAAC 
ACAGTTTGCA CAAATGAAAG AGGCTGGATA ACCTACCAGG GGTTCCTCTC CCAGTGGACG 
CTCACCACCT ACTTAGATGT GCAGCGTTGC CTGGAGTATT TGGGATATCT AGGCTATTCC 
ATACTGACGG AACAAGAGTC TCAAGCTTCA GCCATTACAG TAACAAGGGA TAAAAAGATT 
GACCTGCAGA AAAAACAGAC TCAGAGAAAT GTGTTCAGGT GTAATGTAAT TGGAGTGAAA 
GGCTGTGGGA AAACTGGAGT TCTTCAGTCT CTCCTTGGAA GAAACCTAAT GAGGCAGAAG 
AAAATTCGTG ATGATCATAA GTCCTATTAT GCAATTAACA CTGTCTATGT ATATGGACAA 
GAGAAATACT TGTTGCTGCA CGATATCTCA GAGTCGGAAT TTCTAACTGA GGCTGAGACC 
ATTTGTGATG TTGTATGCCT GGTATATGAT GTCACCAATC CCAAATCCTT TGAATACTGT 
GCCAGGATTT TTAAGCAACA TTTTATGGAC AGCAGAATTC CCTGCTTGAT TGTAGCTGCA 
AAGTCAGACC TGCATGAAGT CAAACAGGAA CATAGCATCT CACCTACTGA TTTCTGCAGG 
AAACACAAAA TGCCTCCACC TCAAGCCTTC ACTTGCAATA CTGCTGATGC CCCCAGTAAG 
GATATCTTTG TTAAGTTGAC AACAATGGCC ATGTACCCCC ATGCCCGGTT ACGCTGCATG 
TGCACCTGCA ACAGGTGTAC ATTTTGCATC TGTCAGAACT TCCTCAACTC AGACTTGCTG 
CAATCTGTAA AGAACAAAAT CTTCACTGCA GTTCTTAACA GGTTAAAAGC CCGGGTAGCT 
GAGTGGGGTG CCATGCTGTT AGCAGACGAC GAAGGCAGTA TTACTCACCA GGTGCACGCC 
GTCAGCTCCG TAGAAGATTC CATCTTCATG GAGTCATCTC ACGGTCAGCT TTTTCTTGAT 
GACAGGCACG TGACACAAGC TGACCTCAAG AGCTCAACGT TCTGGCTTCG CGCAAGCTTT 
GGTGCTACGG TCTTTGCAGT CGTGGGCTTT GCTATGTACA GAGCACTACT GAAACAGCGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Rhot1
Entrez Gene ID	59040
Full Name	ras homolog family member T1
Synonyms	2210403N23Rik,AA415293,AF244542,AI834919,Arht1,C430039G08Rik,Miro1
General protein information	Preferred Names ras homolog family member T1 Names mitochondrial Rho GTPase 1 MIRO-1 ras homolog gene family, member T1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B5\|11 47.62 cM

Homo sapiens (human)	RHOT1	NP_001028738.1
Mus musculus (house mouse)	Rhot1	NP_001156827.1
Danio rerio (zebrafish)	rhot1a	NP_997869.1
Caenorhabditis elegans (roundworm)	miro-1	NP_500620.1
Gallus gallus (chicken)	RHOT1	NP_001006208.1
Danio rerio (zebrafish)	LOC100535268	XP_003198159.1
Arabidopsis thaliana (thale cress)	MIRO2	NP_567139.1
Pan troglodytes (chimpanzee)	RHOT1	XP_511399.3
	LOC717901	XP_002800404.1
Xenopus tropicalis (tropical clawed frog)	LOC100492251	XP_004918458.1
Bos taurus (cattle)	RHOT1	NP_001039547.1
Canis lupus familiaris (dog)	RHOT1	XP_003435351.1
Rattus norvegicus (Norway rat)	Rhot1	NP_001100496.1
Drosophila melanogaster (fruit fly)	Miro	NP_732936.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007998	XP_317471.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Rhot1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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