RHOT1A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	RHOT1A
Entrez Gene ID	327143
Full Name	ras homolog family member T1a
Synonyms	rhot1,wu:fd14e11,wu:fi14a05,zgc:55581,zgc:77063
General protein information	Preferred Names ras homolog family member T1a Names mitochondrial Rho GTPase 1-A MIRO-1-A ras homolog gene family member T1-A ras homolog gene family, member T1 ras homolog gene family, member T1a
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009299425.3	XP_009297700.1	mitochondrial Rho GTPase 1-A isoform X1
XM_009299427.3	XP_009297702.1	mitochondrial Rho GTPase 1-A isoform X3
XM_009299427.2	XP_009297702.1	mitochondrial Rho GTPase 1-A isoform X3
XM_017353420.1	XP_017208909.1	mitochondrial Rho GTPase 1-A isoform X4
NM_212704.1	NP_997869.1	mitochondrial Rho GTPase 1-A
XM_009299426.3	XP_009297701.1	mitochondrial Rho GTPase 1-A isoform X2
XM_009299425.2	XP_009297700.1	mitochondrial Rho GTPase 1-A isoform X1
XM_009299426.2	XP_009297701.1	mitochondrial Rho GTPase 1-A isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	RHOT1	NP_001028738.1
Mus musculus (house mouse)	Rhot1	NP_001156827.1
Danio rerio (zebrafish)	rhot1a	NP_997869.1
Caenorhabditis elegans (roundworm)	miro-1	NP_500620.1
Gallus gallus (chicken)	RHOT1	NP_001006208.1
Danio rerio (zebrafish)	LOC100535268	XP_003198159.1
Arabidopsis thaliana (thale cress)	MIRO2	NP_567139.1
Pan troglodytes (chimpanzee)	RHOT1	XP_511399.3
	LOC717901	XP_002800404.1
Xenopus tropicalis (tropical clawed frog)	LOC100492251	XP_004918458.1
Bos taurus (cattle)	RHOT1	NP_001039547.1
Canis lupus familiaris (dog)	RHOT1	XP_003435351.1
Rattus norvegicus (Norway rat)	Rhot1	NP_001100496.1
Drosophila melanogaster (fruit fly)	Miro	NP_732936.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007998	XP_317471.3

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Targeted Gene Expression in Zebrafish Exposed to Chlorpyrifos-Oxon Confirms Phenotype-Specific Mechanisms Leading to Adverse Outcomes.
Garcia-Reyero N, Escalon L, Prats E, Faria M, Soares AM, Rald?a D
Bulletin of environmental contamination and toxicology96(6)707-13(2016 Jun)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Characterization of the three zebrafish orthologs of the mitochondrial GTPase Miro/Rhot.
Hollister BM, Oonk KA, Weiser DC, Walsh S
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology191126-34(2016 Jan)

Xmrk, kras and myc transgenic zebrafish liver cancer models share molecular signatures with subsets of human hepatocellular carcinoma.
Zheng W, Li Z, Nguyen AT, Li C, Emelyanov A, Gong Z
PloS one9(3)e91179(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RHOT1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RHOT1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03231	NM_212704.1 Latest version!	Danio rerio ras homolog family member T1a (rhot1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa39898	XM_009299426.3 Latest version!	Danio rerio ras homolog family member T1a (rhot1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa39899	XM_009299427.3 Latest version!	Danio rerio ras homolog family member T1a (rhot1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa39897	XM_009299425.3 Latest version!	Danio rerio ras homolog family member T1a (rhot1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa58023	XM_017353420.1 Latest version!	Danio rerio ras homolog family member T1a (rhot1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
ODa39899	XM_009299427.2	Danio rerio ras homolog family member T1a (rhot1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa39897	XM_009299425.2	Danio rerio ras homolog family member T1a (rhot1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa39898	XM_009299426.2	Danio rerio ras homolog family member T1a (rhot1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RHOT1A ( NM_212704.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_997869.1 Danio rerio ras homolog family member T1a (rhot1a), mRNA.

Price & Availability↑

CloneID	ODa03231
Clone ID Related Accession (Same CDS sequence)	NM_212704.1
Accession Version	NM_212704.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	mitochondrial Rho GTPase 1-A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC068190.1. ##Evidence-Data-START## Transcript exon combination :: BC068190.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGAAAGG ACGTGAGGAT ATTACTGGTC GGAGAACCCA AAGTGGGGAA GACCTCTCTT 
ATCATGTCAC TCGTCAGCGA GGAATTTCCT GATGAGGTTC CTCCAAGAGC CGAAGAGATT 
ACTATTCCTG CAGACGTCAC ACCTGAGCGA GTGCCCACTC ATATAGTAGA TTACTCCGAA 
GCAGAACAGT CAGATGAGCA GCTTTACCAA GAAATAACAA AGGCTAATGT TATATGCATC 
GTCTATTCAG TGAACAATAA GAAATCAATC GAAAAAGTGA CGAGTCATTG GATTCCACTC 
ATCAATGAAA GAACAGATAA GGACAGCAGA GTGCCGCTAA TCCTGGTGGG CAATAAATCT 
GACCTGGTGG AGCACAGCAG CATGGAGACC ATCCTGCCCA TCATGAACCA GTACTCAGAG 
ATTGAGACCT GTGTGGAGTG TTCTGCCAAA AACCTGAAGA ATATCTCTGA GCTGTTTTAT 
TATGCCCAGA AGGCTGTTCT TCACCCCACA GGACCTCTGT ACTCGCCAGA AGAGAAAGAG 
ATGAAGCCGT CTTGCATCAA AGCACTCACT CGCATTTTCA AAATATCAGA CCTGGATAAT 
GATGGCATTC TGAATGATAA CGAGCTTAAT TTCTTCCAGA GAACATGTTT TAACATACCA 
CTGGCACCTC AAGCCCTGGA GGACGTCAAG AATGTCGTGC GGAAAAATAT GACCGATGGA 
GTCAAAGACA ACGGCCTTAC ACTGAAGGGT TTCCTGTTTC TTCACACACT CTTCATTCAG 
AGGGGGAGGC ATGAGACCAC ATGGACCGTG CTTAGGAGGT TTGGTTATGA TGATGATCTG 
GAGCTTACAC AAGAATACCT GTTTCCACTG TTTAAAATAC CTCCAGACTG CACAACAGAG 
CTGAATCACA ATGCCTACCT CTTCCTCCAG AGTGTGTTTG ACAAACATGA CAAAGATCGA 
GACTGTGCCC TGTCTCCAGA TGAGCTGAAG GATTTGTTCA AGGTGTTTCC CTACATGCCC 
TGGGGTCCCG ATGTCAACAA CACAGTCTGC ACCAATGAGC AAGGCTGGAT CACTTACCAA 
GGCTACCTTT CCCAGTGGAC ACTTACAACA TACCTAGATG TGCAGCGGTG TTTGGAGTAC 
CTGGGATATC TCGGCTACTC TATTATTCAA GAGCAGGAGT CACAAGCAGC TGCCATCACA 
GTCACCAGGA ACAAGCGTAT TGACCTGCAG AAGAAGCAGA CCCAGCGCAG TGTTTTCCGA 
TGTAATGTCC TCGGGGCTCG AGGCTGTGGT AAAAGTGGCT TTCTGCAGGC TTTCCTGGGC 
AGGAACCTTG TGCGCCAGAA GAGGATAAGA GAAGACCACA AGTCTTATTA TGCCATTAGC 
ACTACTTATG TTTATGGACA GGAGAAATAT CTGCTCCTTC ACGAGGTGCT TCCAGACGTG 
GAGTTTCTTT CTGAAGCTGA TCTGGCTTGT GATGTTGTTT GTCTGGTTTA TGACATCAGC 
AACCCACGCT CATTTGAATA CTGCGCTAAA GTCTACAAGA AACACTTCAT GGACAGCAAG 
ACCCCATGTG TGATCATTGC TGCCAAGTCT GACCTGCATG AAGCCCGTCA GTACTACAGT 
CTCTCGCCGT TGGACTTCTG CCGAAAACAC AAGCTTCACC CGCCGCAGCT GTTCACCTGC 
AACACCACCG AAGCTCCGAG CAAGGACCTC TACACCAAAC TCACCACTAT GGCCATGTAC 
CCACACATGA CCCAAGCTGA CCTGAAAAAC TCTACATTTT GGCTGAGGGC GAGCGTCGGT 
GCCACTGTCT TTGCTGTTCT GGGCTTTGCC ATGTACAAAG CTCTCCTCAA GCAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997869.1
CDS	306..2165
Translation	MRKDVRILLVGEPKVGKTSLIMSLVSEEFPDEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLYQEITKANVICIVYSVNNKKSIEKVTSHWIPLINERTDKDSRVPLILVGNKSDLVEHSSMETILPIMNQYSEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYSPEEKEMKPSCIKALTRIFKISDLDNDGILNDNELNFFQRTCFNIPLAPQALEDVKNVVRKNMTDGVKDNGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLELTQEYLFPLFKIPPDCTTELNHNAYLFLQSVFDKHDKDRDCALSPDELKDLFKVFPYMPWGPDVNNTVCTNEQGWITYQGYLSQWTLTTYLDVQRCLEYLGYLGYSIIQEQESQAAAITVTRNKRIDLQKKQTQRSVFRCNVLGARGCGKSGFLQAFLGRNLVRQKRIREDHKSYYAISTTYVYGQEKYLLLHEVLPDVEFLSEADLACDVVCLVYDISNPRSFEYCAKVYKKHFMDSKTPCVIIAAKSDLHEARQYYSLSPLDFCRKHKLHPPQLFTCNTTEAPSKDLYTKLTTMAMYPHMTQADLKNSTFWLRASVGATVFAVLGFAMYKALLKQR

Target ORF information:

RefSeq Version	NM_212704.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ras homolog family member T1a (rhot1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212704.1

ORF Insert Sequence:

ATGAGAAAGG ACGTGAGGAT ATTACTGGTC GGAGAACCCA AAGTGGGGAA GACCTCTCTT 
ATCATGTCAC TCGTCAGCGA GGAATTTCCT GATGAGGTTC CTCCAAGAGC CGAAGAGATT 
ACTATTCCTG CAGACGTCAC ACCTGAGCGA GTGCCCACTC ATATAGTAGA TTACTCCGAA 
GCAGAACAGT CAGATGAGCA GCTTTACCAA GAAATAACAA AGGCTAATGT TATATGCATC 
GTCTATTCAG TGAACAATAA GAAATCAATC GAAAAAGTGA CGAGTCATTG GATTCCACTC 
ATCAATGAAA GAACAGATAA GGACAGCAGA GTGCCGCTAA TCCTGGTGGG CAATAAATCT 
GACCTGGTGG AGCACAGCAG CATGGAGACC ATCCTGCCCA TCATGAACCA GTACTCAGAG 
ATTGAGACCT GTGTGGAGTG TTCTGCCAAA AACCTGAAGA ATATCTCTGA GCTGTTTTAT 
TATGCCCAGA AGGCTGTTCT TCACCCCACA GGACCTCTGT ACTCGCCAGA AGAGAAAGAG 
ATGAAGCCGT CTTGCATCAA AGCACTCACT CGCATTTTCA AAATATCAGA CCTGGATAAT 
GATGGCATTC TGAATGATAA CGAGCTTAAT TTCTTCCAGA GAACATGTTT TAACATACCA 
CTGGCACCTC AAGCCCTGGA GGACGTCAAG AATGTCGTGC GGAAAAATAT GACCGATGGA 
GTCAAAGACA ACGGCCTTAC ACTGAAGGGT TTCCTGTTTC TTCACACACT CTTCATTCAG 
AGGGGGAGGC ATGAGACCAC ATGGACCGTG CTTAGGAGGT TTGGTTATGA TGATGATCTG 
GAGCTTACAC AAGAATACCT GTTTCCACTG TTTAAAATAC CTCCAGACTG CACAACAGAG 
CTGAATCACA ATGCCTACCT CTTCCTCCAG AGTGTGTTTG ACAAACATGA CAAAGATCGA 
GACTGTGCCC TGTCTCCAGA TGAGCTGAAG GATTTGTTCA AGGTGTTTCC CTACATGCCC 
TGGGGTCCCG ATGTCAACAA CACAGTCTGC ACCAATGAGC AAGGCTGGAT CACTTACCAA 
GGCTACCTTT CCCAGTGGAC ACTTACAACA TACCTAGATG TGCAGCGGTG TTTGGAGTAC 
CTGGGATATC TCGGCTACTC TATTATTCAA GAGCAGGAGT CACAAGCAGC TGCCATCACA 
GTCACCAGGA ACAAGCGTAT TGACCTGCAG AAGAAGCAGA CCCAGCGCAG TGTTTTCCGA 
TGTAATGTCC TCGGGGCTCG AGGCTGTGGT AAAAGTGGCT TTCTGCAGGC TTTCCTGGGC 
AGGAACCTTG TGCGCCAGAA GAGGATAAGA GAAGACCACA AGTCTTATTA TGCCATTAGC 
ACTACTTATG TTTATGGACA GGAGAAATAT CTGCTCCTTC ACGAGGTGCT TCCAGACGTG 
GAGTTTCTTT CTGAAGCTGA TCTGGCTTGT GATGTTGTTT GTCTGGTTTA TGACATCAGC 
AACCCACGCT CATTTGAATA CTGCGCTAAA GTCTACAAGA AACACTTCAT GGACAGCAAG 
ACCCCATGTG TGATCATTGC TGCCAAGTCT GACCTGCATG AAGCCCGTCA GTACTACAGT 
CTCTCGCCGT TGGACTTCTG CCGAAAACAC AAGCTTCACC CGCCGCAGCT GTTCACCTGC 
AACACCACCG AAGCTCCGAG CAAGGACCTC TACACCAAAC TCACCACTAT GGCCATGTAC 
CCACACATGA CCCAAGCTGA CCTGAAAAAC TCTACATTTT GGCTGAGGGC GAGCGTCGGT 
GCCACTGTCT TTGCTGTTCT GGGCTTTGCC ATGTACAAAG CTCTCCTCAA GCAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHOT1A ( XM_009299426.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009297701.1 Danio rerio ras homolog family member T1a (rhot1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa39898
Clone ID Related Accession (Same CDS sequence)	XM_009299426.3 , XM_009299426.2
Accession Version	XM_009299426.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mitochondrial Rho GTPase 1-A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299426.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGG ACGTGAGGAT ATTACTGGTC GGAGAACCCA AAGTGGGGAA GACCTCTCTT 
ATCATGTCAC TCGTCAGCGA GGAATTTCCT GATGAGGTTC CTCCAAGAGC CGAAGAGATT 
ACTATTCCTG CAGACGTCAC ACCTGAGCGA GTGCCCACTC ATATAGTAGA TTACTCCGAA 
GCAGAACAGT CAGATGAGCA GCTTTACCAA GAAATAACAA AGGCTAATGT TATATGCATC 
GTCTATTCAG TGAACAATAA GAAATCAATC GAAAAAGTGA CGAGTCATTG GATTCCACTC 
ATCAATGAAA GAACAGATAA GGACAGCAGA GTGCCGCTAA TCCTGGTGGG CAATAAATCT 
GACCTGGTGG AGCACAGCAG CATGGAGACC ATCCTGCCCA TCATGAACCA GTACTCAGAG 
ATTGAGACCT GTGTGGAGTG TTCTGCCAAA AACCTGAAGA ATATCTCTGA GCTGTTTTAT 
TATGCCCAGA AGGCTGTTCT TCACCCCACA GGACCTCTGT ACTCGCCAGA AGAGAAAGAG 
ATGAAGCCGT CTTGCATCAA AGCACTCACT CGCATTTTCA AAATATCAGA CCTGGATAAT 
GATGGCATTC TGAATGATAA CGAGCTTAAT TTCTTCCAGA GAACATGTTT TAACATACCA 
CTGGCACCTC AAGCCCTGGA GGACGTCAAG AATGTCGTGC GGAAAAATAT GACCGATGGA 
GTCAAAGACA ACGGCCTTAC ACTGAAGGGT TTCCTGTTTC TTCACACACT CTTCATTCAG 
AGGGGGAGGC ATGAGACCAC ATGGACCGTG CTTAGGAGGT TTGGTTATGA TGATGATCTG 
GAGCTTACAC AAGAATACCT GTTTCCACTG TTTAAAATAC CTCCAGACTG CACAACAGAG 
CTGAATCACA ATGCCTACCT CTTCCTCCAG AGTGTGTTTG ACAAACATGA CAAAGATCGA 
GACTGTGCCC TGTCTCCAGA TGAGCTGAAG GATTTGTTCA AGGTGTTTCC CTACATGCCC 
TGGGGTCCCG ATGTCAACAA CACAGTCTGC ACCAATGAGC AAGGCTGGAT CACTTACCAA 
GGCTACCTTT CCCAGTGGAC ACTTACAACA TACCTAGATG TGCAGCGGTG TTTGGAGTAC 
CTGGGATATC TCGGCTACTC TATTATTCAA GAGCAGGAGT CACAAGCAGC TGCCATCACA 
GTCACCAGGA ACAAGCGTAT TGACCTGCAG AAGAAGCAGA CCCAGCGCAG TGTTTTCCGA 
TGTAATGTCC TCGGGGCTCG AGGCTGTGGT AAAAGTGGCT TTCTGCAGGC TTTCCTGGGC 
AGGAACCTTG TGCGCCAGAA GAGGATAAGA GAAGACCACA AGTCTTATTA TGCCATTAGC 
ACTACTTATG TTTATGGACA GGAGAAATAT CTGCTCCTTC ACGAGGTGCT TCCAGACGTG 
GAGTTTCTTT CTGAAGCTGA TCTGGCTTGT GATGTTGTTT GTCTGGTTTA TGACATCAGC 
AACCCACGCT CATTTGAATA CTGCGCTAAA GTCTACAAGA AACACTTCAT GGACAGCAAG 
ACCCCATGTG TGATCATTGC TGCCAAGTCT GACCTGCATG AAGCCCGTCA GTACTACAGT 
CTCTCGCCGT TGGACTTCTG CCGAAAACAC AAGCTTCACC CGCCGCAGCT GTTCACCTGC 
AACACCACCG AAGCTCCGAG CAAGGACCTC TACACCAAAC TCACCACTAT GGCCATGTAC 
CCCCACGCCA AGTTACGCTG CATGTGCGGC TGCAACAGGT GCACCTATTG CATCTGTCAC 
AACCTCCTGA ACTCTGAGCT GGTGCGCTCG GTAAAGGCCA AATTCTCCAG CGCTGTTCTC 
AACAGACACA TGACCCAAGC TGACCTGAAA AACTCTACAT TTTGGCTGAG GGCGAGCGTC 
GGTGCCACTG TCTTTGCTGT TCTGGGCTTT GCCATGTACA AAGCTCTCCT CAAGCAGCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297701.1
CDS	334..2316
Translation	MRKDVRILLVGEPKVGKTSLIMSLVSEEFPDEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLYQEITKANVICIVYSVNNKKSIEKVTSHWIPLINERTDKDSRVPLILVGNKSDLVEHSSMETILPIMNQYSEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYSPEEKEMKPSCIKALTRIFKISDLDNDGILNDNELNFFQRTCFNIPLAPQALEDVKNVVRKNMTDGVKDNGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLELTQEYLFPLFKIPPDCTTELNHNAYLFLQSVFDKHDKDRDCALSPDELKDLFKVFPYMPWGPDVNNTVCTNEQGWITYQGYLSQWTLTTYLDVQRCLEYLGYLGYSIIQEQESQAAAITVTRNKRIDLQKKQTQRSVFRCNVLGARGCGKSGFLQAFLGRNLVRQKRIREDHKSYYAISTTYVYGQEKYLLLHEVLPDVEFLSEADLACDVVCLVYDISNPRSFEYCAKVYKKHFMDSKTPCVIIAAKSDLHEARQYYSLSPLDFCRKHKLHPPQLFTCNTTEAPSKDLYTKLTTMAMYPHAKLRCMCGCNRCTYCICHNLLNSELVRSVKAKFSSAVLNRHMTQADLKNSTFWLRASVGATVFAVLGFAMYKALLKQR

Target ORF information:

RefSeq Version	XM_009299426.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ras homolog family member T1a (rhot1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299426.3

ORF Insert Sequence:

ATGAGAAAGG ACGTGAGGAT ATTACTGGTC GGAGAACCCA AAGTGGGGAA GACCTCTCTT 
ATCATGTCAC TCGTCAGCGA GGAATTTCCT GATGAGGTTC CTCCAAGAGC CGAAGAGATT 
ACTATTCCTG CAGACGTCAC ACCTGAGCGA GTGCCCACTC ATATAGTAGA TTACTCCGAA 
GCAGAACAGT CAGATGAGCA GCTTTACCAA GAAATAACAA AGGCTAATGT TATATGCATC 
GTCTATTCAG TGAACAATAA GAAATCAATC GAAAAAGTGA CGAGTCATTG GATTCCACTC 
ATCAATGAAA GAACAGATAA GGACAGCAGA GTGCCGCTAA TCCTGGTGGG CAATAAATCT 
GACCTGGTGG AGCACAGCAG CATGGAGACC ATCCTGCCCA TCATGAACCA GTACTCAGAG 
ATTGAGACCT GTGTGGAGTG TTCTGCCAAA AACCTGAAGA ATATCTCTGA GCTGTTTTAT 
TATGCCCAGA AGGCTGTTCT TCACCCCACA GGACCTCTGT ACTCGCCAGA AGAGAAAGAG 
ATGAAGCCGT CTTGCATCAA AGCACTCACT CGCATTTTCA AAATATCAGA CCTGGATAAT 
GATGGCATTC TGAATGATAA CGAGCTTAAT TTCTTCCAGA GAACATGTTT TAACATACCA 
CTGGCACCTC AAGCCCTGGA GGACGTCAAG AATGTCGTGC GGAAAAATAT GACCGATGGA 
GTCAAAGACA ACGGCCTTAC ACTGAAGGGT TTCCTGTTTC TTCACACACT CTTCATTCAG 
AGGGGGAGGC ATGAGACCAC ATGGACCGTG CTTAGGAGGT TTGGTTATGA TGATGATCTG 
GAGCTTACAC AAGAATACCT GTTTCCACTG TTTAAAATAC CTCCAGACTG CACAACAGAG 
CTGAATCACA ATGCCTACCT CTTCCTCCAG AGTGTGTTTG ACAAACATGA CAAAGATCGA 
GACTGTGCCC TGTCTCCAGA TGAGCTGAAG GATTTGTTCA AGGTGTTTCC CTACATGCCC 
TGGGGTCCCG ATGTCAACAA CACAGTCTGC ACCAATGAGC AAGGCTGGAT CACTTACCAA 
GGCTACCTTT CCCAGTGGAC ACTTACAACA TACCTAGATG TGCAGCGGTG TTTGGAGTAC 
CTGGGATATC TCGGCTACTC TATTATTCAA GAGCAGGAGT CACAAGCAGC TGCCATCACA 
GTCACCAGGA ACAAGCGTAT TGACCTGCAG AAGAAGCAGA CCCAGCGCAG TGTTTTCCGA 
TGTAATGTCC TCGGGGCTCG AGGCTGTGGT AAAAGTGGCT TTCTGCAGGC TTTCCTGGGC 
AGGAACCTTG TGCGCCAGAA GAGGATAAGA GAAGACCACA AGTCTTATTA TGCCATTAGC 
ACTACTTATG TTTATGGACA GGAGAAATAT CTGCTCCTTC ACGAGGTGCT TCCAGACGTG 
GAGTTTCTTT CTGAAGCTGA TCTGGCTTGT GATGTTGTTT GTCTGGTTTA TGACATCAGC 
AACCCACGCT CATTTGAATA CTGCGCTAAA GTCTACAAGA AACACTTCAT GGACAGCAAG 
ACCCCATGTG TGATCATTGC TGCCAAGTCT GACCTGCATG AAGCCCGTCA GTACTACAGT 
CTCTCGCCGT TGGACTTCTG CCGAAAACAC AAGCTTCACC CGCCGCAGCT GTTCACCTGC 
AACACCACCG AAGCTCCGAG CAAGGACCTC TACACCAAAC TCACCACTAT GGCCATGTAC 
CCCCACGCCA AGTTACGCTG CATGTGCGGC TGCAACAGGT GCACCTATTG CATCTGTCAC 
AACCTCCTGA ACTCTGAGCT GGTGCGCTCG GTAAAGGCCA AATTCTCCAG CGCTGTTCTC 
AACAGACACA TGACCCAAGC TGACCTGAAA AACTCTACAT TTTGGCTGAG GGCGAGCGTC 
GGTGCCACTG TCTTTGCTGT TCTGGGCTTT GCCATGTACA AAGCTCTCCT CAAGCAGCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHOT1A ( XM_009299426.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009297701.1 Danio rerio ras homolog family member T1a (rhot1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa39898
Clone ID Related Accession (Same CDS sequence)	XM_009299426.3 , XM_009299426.2
Accession Version	XM_009299426.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mitochondrial Rho GTPase 1-A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299426.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGG ACGTGAGGAT ATTACTGGTC GGAGAACCCA AAGTGGGGAA GACCTCTCTT 
ATCATGTCAC TCGTCAGCGA GGAATTTCCT GATGAGGTTC CTCCAAGAGC CGAAGAGATT 
ACTATTCCTG CAGACGTCAC ACCTGAGCGA GTGCCCACTC ATATAGTAGA TTACTCCGAA 
GCAGAACAGT CAGATGAGCA GCTTTACCAA GAAATAACAA AGGCTAATGT TATATGCATC 
GTCTATTCAG TGAACAATAA GAAATCAATC GAAAAAGTGA CGAGTCATTG GATTCCACTC 
ATCAATGAAA GAACAGATAA GGACAGCAGA GTGCCGCTAA TCCTGGTGGG CAATAAATCT 
GACCTGGTGG AGCACAGCAG CATGGAGACC ATCCTGCCCA TCATGAACCA GTACTCAGAG 
ATTGAGACCT GTGTGGAGTG TTCTGCCAAA AACCTGAAGA ATATCTCTGA GCTGTTTTAT 
TATGCCCAGA AGGCTGTTCT TCACCCCACA GGACCTCTGT ACTCGCCAGA AGAGAAAGAG 
ATGAAGCCGT CTTGCATCAA AGCACTCACT CGCATTTTCA AAATATCAGA CCTGGATAAT 
GATGGCATTC TGAATGATAA CGAGCTTAAT TTCTTCCAGA GAACATGTTT TAACATACCA 
CTGGCACCTC AAGCCCTGGA GGACGTCAAG AATGTCGTGC GGAAAAATAT GACCGATGGA 
GTCAAAGACA ACGGCCTTAC ACTGAAGGGT TTCCTGTTTC TTCACACACT CTTCATTCAG 
AGGGGGAGGC ATGAGACCAC ATGGACCGTG CTTAGGAGGT TTGGTTATGA TGATGATCTG 
GAGCTTACAC AAGAATACCT GTTTCCACTG TTTAAAATAC CTCCAGACTG CACAACAGAG 
CTGAATCACA ATGCCTACCT CTTCCTCCAG AGTGTGTTTG ACAAACATGA CAAAGATCGA 
GACTGTGCCC TGTCTCCAGA TGAGCTGAAG GATTTGTTCA AGGTGTTTCC CTACATGCCC 
TGGGGTCCCG ATGTCAACAA CACAGTCTGC ACCAATGAGC AAGGCTGGAT CACTTACCAA 
GGCTACCTTT CCCAGTGGAC ACTTACAACA TACCTAGATG TGCAGCGGTG TTTGGAGTAC 
CTGGGATATC TCGGCTACTC TATTATTCAA GAGCAGGAGT CACAAGCAGC TGCCATCACA 
GTCACCAGGA ACAAGCGTAT TGACCTGCAG AAGAAGCAGA CCCAGCGCAG TGTTTTCCGA 
TGTAATGTCC TCGGGGCTCG AGGCTGTGGT AAAAGTGGCT TTCTGCAGGC TTTCCTGGGC 
AGGAACCTTG TGCGCCAGAA GAGGATAAGA GAAGACCACA AGTCTTATTA TGCCATTAGC 
ACTACTTATG TTTATGGACA GGAGAAATAT CTGCTCCTTC ACGAGGTGCT TCCAGACGTG 
GAGTTTCTTT CTGAAGCTGA TCTGGCTTGT GATGTTGTTT GTCTGGTTTA TGACATCAGC 
AACCCACGCT CATTTGAATA CTGCGCTAAA GTCTACAAGA AACACTTCAT GGACAGCAAG 
ACCCCATGTG TGATCATTGC TGCCAAGTCT GACCTGCATG AAGCCCGTCA GTACTACAGT 
CTCTCGCCGT TGGACTTCTG CCGAAAACAC AAGCTTCACC CGCCGCAGCT GTTCACCTGC 
AACACCACCG AAGCTCCGAG CAAGGACCTC TACACCAAAC TCACCACTAT GGCCATGTAC 
CCCCACGCCA AGTTACGCTG CATGTGCGGC TGCAACAGGT GCACCTATTG CATCTGTCAC 
AACCTCCTGA ACTCTGAGCT GGTGCGCTCG GTAAAGGCCA AATTCTCCAG CGCTGTTCTC 
AACAGACACA TGACCCAAGC TGACCTGAAA AACTCTACAT TTTGGCTGAG GGCGAGCGTC 
GGTGCCACTG TCTTTGCTGT TCTGGGCTTT GCCATGTACA AAGCTCTCCT CAAGCAGCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297701.1
CDS	330..2312
Translation	MRKDVRILLVGEPKVGKTSLIMSLVSEEFPDEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLYQEITKANVICIVYSVNNKKSIEKVTSHWIPLINERTDKDSRVPLILVGNKSDLVEHSSMETILPIMNQYSEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYSPEEKEMKPSCIKALTRIFKISDLDNDGILNDNELNFFQRTCFNIPLAPQALEDVKNVVRKNMTDGVKDNGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLELTQEYLFPLFKIPPDCTTELNHNAYLFLQSVFDKHDKDRDCALSPDELKDLFKVFPYMPWGPDVNNTVCTNEQGWITYQGYLSQWTLTTYLDVQRCLEYLGYLGYSIIQEQESQAAAITVTRNKRIDLQKKQTQRSVFRCNVLGARGCGKSGFLQAFLGRNLVRQKRIREDHKSYYAISTTYVYGQEKYLLLHEVLPDVEFLSEADLACDVVCLVYDISNPRSFEYCAKVYKKHFMDSKTPCVIIAAKSDLHEARQYYSLSPLDFCRKHKLHPPQLFTCNTTEAPSKDLYTKLTTMAMYPHAKLRCMCGCNRCTYCICHNLLNSELVRSVKAKFSSAVLNRHMTQADLKNSTFWLRASVGATVFAVLGFAMYKALLKQR

Target ORF information:

RefSeq Version	XM_009299426.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ras homolog family member T1a (rhot1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299426.2

ORF Insert Sequence:

ATGAGAAAGG ACGTGAGGAT ATTACTGGTC GGAGAACCCA AAGTGGGGAA GACCTCTCTT 
ATCATGTCAC TCGTCAGCGA GGAATTTCCT GATGAGGTTC CTCCAAGAGC CGAAGAGATT 
ACTATTCCTG CAGACGTCAC ACCTGAGCGA GTGCCCACTC ATATAGTAGA TTACTCCGAA 
GCAGAACAGT CAGATGAGCA GCTTTACCAA GAAATAACAA AGGCTAATGT TATATGCATC 
GTCTATTCAG TGAACAATAA GAAATCAATC GAAAAAGTGA CGAGTCATTG GATTCCACTC 
ATCAATGAAA GAACAGATAA GGACAGCAGA GTGCCGCTAA TCCTGGTGGG CAATAAATCT 
GACCTGGTGG AGCACAGCAG CATGGAGACC ATCCTGCCCA TCATGAACCA GTACTCAGAG 
ATTGAGACCT GTGTGGAGTG TTCTGCCAAA AACCTGAAGA ATATCTCTGA GCTGTTTTAT 
TATGCCCAGA AGGCTGTTCT TCACCCCACA GGACCTCTGT ACTCGCCAGA AGAGAAAGAG 
ATGAAGCCGT CTTGCATCAA AGCACTCACT CGCATTTTCA AAATATCAGA CCTGGATAAT 
GATGGCATTC TGAATGATAA CGAGCTTAAT TTCTTCCAGA GAACATGTTT TAACATACCA 
CTGGCACCTC AAGCCCTGGA GGACGTCAAG AATGTCGTGC GGAAAAATAT GACCGATGGA 
GTCAAAGACA ACGGCCTTAC ACTGAAGGGT TTCCTGTTTC TTCACACACT CTTCATTCAG 
AGGGGGAGGC ATGAGACCAC ATGGACCGTG CTTAGGAGGT TTGGTTATGA TGATGATCTG 
GAGCTTACAC AAGAATACCT GTTTCCACTG TTTAAAATAC CTCCAGACTG CACAACAGAG 
CTGAATCACA ATGCCTACCT CTTCCTCCAG AGTGTGTTTG ACAAACATGA CAAAGATCGA 
GACTGTGCCC TGTCTCCAGA TGAGCTGAAG GATTTGTTCA AGGTGTTTCC CTACATGCCC 
TGGGGTCCCG ATGTCAACAA CACAGTCTGC ACCAATGAGC AAGGCTGGAT CACTTACCAA 
GGCTACCTTT CCCAGTGGAC ACTTACAACA TACCTAGATG TGCAGCGGTG TTTGGAGTAC 
CTGGGATATC TCGGCTACTC TATTATTCAA GAGCAGGAGT CACAAGCAGC TGCCATCACA 
GTCACCAGGA ACAAGCGTAT TGACCTGCAG AAGAAGCAGA CCCAGCGCAG TGTTTTCCGA 
TGTAATGTCC TCGGGGCTCG AGGCTGTGGT AAAAGTGGCT TTCTGCAGGC TTTCCTGGGC 
AGGAACCTTG TGCGCCAGAA GAGGATAAGA GAAGACCACA AGTCTTATTA TGCCATTAGC 
ACTACTTATG TTTATGGACA GGAGAAATAT CTGCTCCTTC ACGAGGTGCT TCCAGACGTG 
GAGTTTCTTT CTGAAGCTGA TCTGGCTTGT GATGTTGTTT GTCTGGTTTA TGACATCAGC 
AACCCACGCT CATTTGAATA CTGCGCTAAA GTCTACAAGA AACACTTCAT GGACAGCAAG 
ACCCCATGTG TGATCATTGC TGCCAAGTCT GACCTGCATG AAGCCCGTCA GTACTACAGT 
CTCTCGCCGT TGGACTTCTG CCGAAAACAC AAGCTTCACC CGCCGCAGCT GTTCACCTGC 
AACACCACCG AAGCTCCGAG CAAGGACCTC TACACCAAAC TCACCACTAT GGCCATGTAC 
CCCCACGCCA AGTTACGCTG CATGTGCGGC TGCAACAGGT GCACCTATTG CATCTGTCAC 
AACCTCCTGA ACTCTGAGCT GGTGCGCTCG GTAAAGGCCA AATTCTCCAG CGCTGTTCTC 
AACAGACACA TGACCCAAGC TGACCTGAAA AACTCTACAT TTTGGCTGAG GGCGAGCGTC 
GGTGCCACTG TCTTTGCTGT TCTGGGCTTT GCCATGTACA AAGCTCTCCT CAAGCAGCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHOT1A ( XM_009299427.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009297702.1 Danio rerio ras homolog family member T1a (rhot1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa39899
Clone ID Related Accession (Same CDS sequence)	XM_009299427.3 , XM_009299427.2
Accession Version	XM_009299427.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mitochondrial Rho GTPase 1-A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299427.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGG ACGTGAGGAT ATTACTGGTC GGAGAACCCA AAGTGGGGAA GACCTCTCTT 
ATCATGTCAC TCGTCAGCGA GGAATTTCCT GATGAGGTTC CTCCAAGAGC CGAAGAGATT 
ACTATTCCTG CAGACGTCAC ACCTGAGCGA GTGCCCACTC ATATAGTAGA TTACTCCGAA 
GCAGAACAGT CAGATGAGCA GCTTTACCAA GAAATAACAA AGGCTAATGT TATATGCATC 
GTCTATTCAG TGAACAATAA GAAATCAATC GAAAAAGTGA CGAGTCATTG GATTCCACTC 
ATCAATGAAA GAACAGATAA GGACAGCAGA GTGCCGCTAA TCCTGGTGGG CAATAAATCT 
GACCTGGTGG AGCACAGCAG CATGGAGACC ATCCTGCCCA TCATGAACCA GTACTCAGAG 
ATTGAGACCT GTGTGGAGTG TTCTGCCAAA AACCTGAAGA ATATCTCTGA GCTGTTTTAT 
TATGCCCAGA AGGCTGTTCT TCACCCCACA GGACCTCTGT ACTCGCCAGA AGAGAAAGAG 
ATGAAGCCGT CTTGCATCAA AGCACTCACT CGCATTTTCA AAATATCAGA CCTGGATAAT 
GATGGCATTC TGAATGATAA CGAGCTTAAT TTCTTCCAGA GAACATGTTT TAACATACCA 
CTGGCACCTC AAGCCCTGGA GGACGTCAAG AATGTCGTGC GGAAAAATAT GACCGATGGA 
GTCAAAGACA ACGGCCTTAC ACTGAAGGGT TTCCTGTTTC TTCACACACT CTTCATTCAG 
AGGGGGAGGC ATGAGACCAC ATGGACCGTG CTTAGGAGGT TTGGTTATGA TGATGATCTG 
GAGCTTACAC AAGAATACCT GTTTCCACTG TTTAAAATAC CTCCAGACTG CACAACAGAG 
CTGAATCACA ATGCCTACCT CTTCCTCCAG AGTGTGTTTG ACAAACATGA CAAAGATCGA 
GACTGTGCCC TGTCTCCAGA TGAGCTGAAG GATTTGTTCA AGGTGTTTCC CTACATGCCC 
TGGGGTCCCG ATGTCAACAA CACAGTCTGC ACCAATGAGC AAGGCTGGAT CACTTACCAA 
GGCTACCTTT CCCAGTGGAC ACTTACAACA TACCTAGATG TGCAGCGGTG TTTGGAGTAC 
CTGGGATATC TCGGCTACTC TATTATTCAA GAGCAGGAGT CACAAGCAGC TGCCATCACA 
GTCACCAGGA ACAAGCGTAT TGACCTGCAG AAGAAGCAGA CCCAGCGCAG TGTTTTCCGA 
TGTAATGTCC TCGGGGCTCG AGGCTGTGGT AAAAGTGGCT TTCTGCAGGC TTTCCTGGGC 
AGGAACCTTG TGCGCCAGAA GAGGATAAGA GAAGACCACA AGTCTTATTA TGCCATTAGC 
ACTACTTATG TTTATGGACA GGAGAAATAT CTGCTCCTTC ACGAGGTGCT TCCAGACGTG 
GAGTTTCTTT CTGAAGCTGA TCTGGCTTGT GATGTTGTTT GTCTGGTTTA TGACATCAGC 
AACCCACGCT CATTTGAATA CTGCGCTAAA GTCTACAAGA AACACTTCAT GGACAGCAAG 
ACCCCATGTG TGATCATTGC TGCCAAGTCT GACCTGCATG AAGCCCGTCA GTACTACAGT 
CTCTCGCCGT TGGACTTCTG CCGAAAACAC AAGCTTCACC CGCCGCAGCT GTTCACCTGC 
AACACCACCG AAGCTCCGAG CAAGGACCTC TACACCAAAC TCACCACTAT GGCCATGTAC 
CCCCACGCCA AGTTACGCTG CATGTGCGGC TGCAACAGGT GCACCTATTG CATCTGTCAC 
AACCTCCTGA ACTCTGAGCT GGTGCGCTCG GTAAAGGCCA AATTCTCCAG CGCTGTTCTC 
AACAGGTGTT CCTTTTCATT TGTTTGGCTG GCCCTTTTCC TCTTTGCTTT GCATCTTTTC 
ACATGCTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297702.1
CDS	335..2266
Translation	MRKDVRILLVGEPKVGKTSLIMSLVSEEFPDEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLYQEITKANVICIVYSVNNKKSIEKVTSHWIPLINERTDKDSRVPLILVGNKSDLVEHSSMETILPIMNQYSEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYSPEEKEMKPSCIKALTRIFKISDLDNDGILNDNELNFFQRTCFNIPLAPQALEDVKNVVRKNMTDGVKDNGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLELTQEYLFPLFKIPPDCTTELNHNAYLFLQSVFDKHDKDRDCALSPDELKDLFKVFPYMPWGPDVNNTVCTNEQGWITYQGYLSQWTLTTYLDVQRCLEYLGYLGYSIIQEQESQAAAITVTRNKRIDLQKKQTQRSVFRCNVLGARGCGKSGFLQAFLGRNLVRQKRIREDHKSYYAISTTYVYGQEKYLLLHEVLPDVEFLSEADLACDVVCLVYDISNPRSFEYCAKVYKKHFMDSKTPCVIIAAKSDLHEARQYYSLSPLDFCRKHKLHPPQLFTCNTTEAPSKDLYTKLTTMAMYPHAKLRCMCGCNRCTYCICHNLLNSELVRSVKAKFSSAVLNRCSFSFVWLALFLFALHLFTCY

Target ORF information:

RefSeq Version	XM_009299427.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ras homolog family member T1a (rhot1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299427.3

ORF Insert Sequence:

ATGAGAAAGG ACGTGAGGAT ATTACTGGTC GGAGAACCCA AAGTGGGGAA GACCTCTCTT 
ATCATGTCAC TCGTCAGCGA GGAATTTCCT GATGAGGTTC CTCCAAGAGC CGAAGAGATT 
ACTATTCCTG CAGACGTCAC ACCTGAGCGA GTGCCCACTC ATATAGTAGA TTACTCCGAA 
GCAGAACAGT CAGATGAGCA GCTTTACCAA GAAATAACAA AGGCTAATGT TATATGCATC 
GTCTATTCAG TGAACAATAA GAAATCAATC GAAAAAGTGA CGAGTCATTG GATTCCACTC 
ATCAATGAAA GAACAGATAA GGACAGCAGA GTGCCGCTAA TCCTGGTGGG CAATAAATCT 
GACCTGGTGG AGCACAGCAG CATGGAGACC ATCCTGCCCA TCATGAACCA GTACTCAGAG 
ATTGAGACCT GTGTGGAGTG TTCTGCCAAA AACCTGAAGA ATATCTCTGA GCTGTTTTAT 
TATGCCCAGA AGGCTGTTCT TCACCCCACA GGACCTCTGT ACTCGCCAGA AGAGAAAGAG 
ATGAAGCCGT CTTGCATCAA AGCACTCACT CGCATTTTCA AAATATCAGA CCTGGATAAT 
GATGGCATTC TGAATGATAA CGAGCTTAAT TTCTTCCAGA GAACATGTTT TAACATACCA 
CTGGCACCTC AAGCCCTGGA GGACGTCAAG AATGTCGTGC GGAAAAATAT GACCGATGGA 
GTCAAAGACA ACGGCCTTAC ACTGAAGGGT TTCCTGTTTC TTCACACACT CTTCATTCAG 
AGGGGGAGGC ATGAGACCAC ATGGACCGTG CTTAGGAGGT TTGGTTATGA TGATGATCTG 
GAGCTTACAC AAGAATACCT GTTTCCACTG TTTAAAATAC CTCCAGACTG CACAACAGAG 
CTGAATCACA ATGCCTACCT CTTCCTCCAG AGTGTGTTTG ACAAACATGA CAAAGATCGA 
GACTGTGCCC TGTCTCCAGA TGAGCTGAAG GATTTGTTCA AGGTGTTTCC CTACATGCCC 
TGGGGTCCCG ATGTCAACAA CACAGTCTGC ACCAATGAGC AAGGCTGGAT CACTTACCAA 
GGCTACCTTT CCCAGTGGAC ACTTACAACA TACCTAGATG TGCAGCGGTG TTTGGAGTAC 
CTGGGATATC TCGGCTACTC TATTATTCAA GAGCAGGAGT CACAAGCAGC TGCCATCACA 
GTCACCAGGA ACAAGCGTAT TGACCTGCAG AAGAAGCAGA CCCAGCGCAG TGTTTTCCGA 
TGTAATGTCC TCGGGGCTCG AGGCTGTGGT AAAAGTGGCT TTCTGCAGGC TTTCCTGGGC 
AGGAACCTTG TGCGCCAGAA GAGGATAAGA GAAGACCACA AGTCTTATTA TGCCATTAGC 
ACTACTTATG TTTATGGACA GGAGAAATAT CTGCTCCTTC ACGAGGTGCT TCCAGACGTG 
GAGTTTCTTT CTGAAGCTGA TCTGGCTTGT GATGTTGTTT GTCTGGTTTA TGACATCAGC 
AACCCACGCT CATTTGAATA CTGCGCTAAA GTCTACAAGA AACACTTCAT GGACAGCAAG 
ACCCCATGTG TGATCATTGC TGCCAAGTCT GACCTGCATG AAGCCCGTCA GTACTACAGT 
CTCTCGCCGT TGGACTTCTG CCGAAAACAC AAGCTTCACC CGCCGCAGCT GTTCACCTGC 
AACACCACCG AAGCTCCGAG CAAGGACCTC TACACCAAAC TCACCACTAT GGCCATGTAC 
CCCCACGCCA AGTTACGCTG CATGTGCGGC TGCAACAGGT GCACCTATTG CATCTGTCAC 
AACCTCCTGA ACTCTGAGCT GGTGCGCTCG GTAAAGGCCA AATTCTCCAG CGCTGTTCTC 
AACAGGTGTT CCTTTTCATT TGTTTGGCTG GCCCTTTTCC TCTTTGCTTT GCATCTTTTC 
ACATGCTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHOT1A ( XM_009299427.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009297702.1 Danio rerio ras homolog family member T1a (rhot1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa39899
Clone ID Related Accession (Same CDS sequence)	XM_009299427.3 , XM_009299427.2
Accession Version	XM_009299427.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mitochondrial Rho GTPase 1-A isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299427.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGG ACGTGAGGAT ATTACTGGTC GGAGAACCCA AAGTGGGGAA GACCTCTCTT 
ATCATGTCAC TCGTCAGCGA GGAATTTCCT GATGAGGTTC CTCCAAGAGC CGAAGAGATT 
ACTATTCCTG CAGACGTCAC ACCTGAGCGA GTGCCCACTC ATATAGTAGA TTACTCCGAA 
GCAGAACAGT CAGATGAGCA GCTTTACCAA GAAATAACAA AGGCTAATGT TATATGCATC 
GTCTATTCAG TGAACAATAA GAAATCAATC GAAAAAGTGA CGAGTCATTG GATTCCACTC 
ATCAATGAAA GAACAGATAA GGACAGCAGA GTGCCGCTAA TCCTGGTGGG CAATAAATCT 
GACCTGGTGG AGCACAGCAG CATGGAGACC ATCCTGCCCA TCATGAACCA GTACTCAGAG 
ATTGAGACCT GTGTGGAGTG TTCTGCCAAA AACCTGAAGA ATATCTCTGA GCTGTTTTAT 
TATGCCCAGA AGGCTGTTCT TCACCCCACA GGACCTCTGT ACTCGCCAGA AGAGAAAGAG 
ATGAAGCCGT CTTGCATCAA AGCACTCACT CGCATTTTCA AAATATCAGA CCTGGATAAT 
GATGGCATTC TGAATGATAA CGAGCTTAAT TTCTTCCAGA GAACATGTTT TAACATACCA 
CTGGCACCTC AAGCCCTGGA GGACGTCAAG AATGTCGTGC GGAAAAATAT GACCGATGGA 
GTCAAAGACA ACGGCCTTAC ACTGAAGGGT TTCCTGTTTC TTCACACACT CTTCATTCAG 
AGGGGGAGGC ATGAGACCAC ATGGACCGTG CTTAGGAGGT TTGGTTATGA TGATGATCTG 
GAGCTTACAC AAGAATACCT GTTTCCACTG TTTAAAATAC CTCCAGACTG CACAACAGAG 
CTGAATCACA ATGCCTACCT CTTCCTCCAG AGTGTGTTTG ACAAACATGA CAAAGATCGA 
GACTGTGCCC TGTCTCCAGA TGAGCTGAAG GATTTGTTCA AGGTGTTTCC CTACATGCCC 
TGGGGTCCCG ATGTCAACAA CACAGTCTGC ACCAATGAGC AAGGCTGGAT CACTTACCAA 
GGCTACCTTT CCCAGTGGAC ACTTACAACA TACCTAGATG TGCAGCGGTG TTTGGAGTAC 
CTGGGATATC TCGGCTACTC TATTATTCAA GAGCAGGAGT CACAAGCAGC TGCCATCACA 
GTCACCAGGA ACAAGCGTAT TGACCTGCAG AAGAAGCAGA CCCAGCGCAG TGTTTTCCGA 
TGTAATGTCC TCGGGGCTCG AGGCTGTGGT AAAAGTGGCT TTCTGCAGGC TTTCCTGGGC 
AGGAACCTTG TGCGCCAGAA GAGGATAAGA GAAGACCACA AGTCTTATTA TGCCATTAGC 
ACTACTTATG TTTATGGACA GGAGAAATAT CTGCTCCTTC ACGAGGTGCT TCCAGACGTG 
GAGTTTCTTT CTGAAGCTGA TCTGGCTTGT GATGTTGTTT GTCTGGTTTA TGACATCAGC 
AACCCACGCT CATTTGAATA CTGCGCTAAA GTCTACAAGA AACACTTCAT GGACAGCAAG 
ACCCCATGTG TGATCATTGC TGCCAAGTCT GACCTGCATG AAGCCCGTCA GTACTACAGT 
CTCTCGCCGT TGGACTTCTG CCGAAAACAC AAGCTTCACC CGCCGCAGCT GTTCACCTGC 
AACACCACCG AAGCTCCGAG CAAGGACCTC TACACCAAAC TCACCACTAT GGCCATGTAC 
CCCCACGCCA AGTTACGCTG CATGTGCGGC TGCAACAGGT GCACCTATTG CATCTGTCAC 
AACCTCCTGA ACTCTGAGCT GGTGCGCTCG GTAAAGGCCA AATTCTCCAG CGCTGTTCTC 
AACAGGTGTT CCTTTTCATT TGTTTGGCTG GCCCTTTTCC TCTTTGCTTT GCATCTTTTC 
ACATGCTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297702.1
CDS	332..2263
Translation	MRKDVRILLVGEPKVGKTSLIMSLVSEEFPDEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLYQEITKANVICIVYSVNNKKSIEKVTSHWIPLINERTDKDSRVPLILVGNKSDLVEHSSMETILPIMNQYSEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYSPEEKEMKPSCIKALTRIFKISDLDNDGILNDNELNFFQRTCFNIPLAPQALEDVKNVVRKNMTDGVKDNGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLELTQEYLFPLFKIPPDCTTELNHNAYLFLQSVFDKHDKDRDCALSPDELKDLFKVFPYMPWGPDVNNTVCTNEQGWITYQGYLSQWTLTTYLDVQRCLEYLGYLGYSIIQEQESQAAAITVTRNKRIDLQKKQTQRSVFRCNVLGARGCGKSGFLQAFLGRNLVRQKRIREDHKSYYAISTTYVYGQEKYLLLHEVLPDVEFLSEADLACDVVCLVYDISNPRSFEYCAKVYKKHFMDSKTPCVIIAAKSDLHEARQYYSLSPLDFCRKHKLHPPQLFTCNTTEAPSKDLYTKLTTMAMYPHAKLRCMCGCNRCTYCICHNLLNSELVRSVKAKFSSAVLNRCSFSFVWLALFLFALHLFTCY

Target ORF information:

RefSeq Version	XM_009299427.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ras homolog family member T1a (rhot1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299427.2

ORF Insert Sequence:

ATGAGAAAGG ACGTGAGGAT ATTACTGGTC GGAGAACCCA AAGTGGGGAA GACCTCTCTT 
ATCATGTCAC TCGTCAGCGA GGAATTTCCT GATGAGGTTC CTCCAAGAGC CGAAGAGATT 
ACTATTCCTG CAGACGTCAC ACCTGAGCGA GTGCCCACTC ATATAGTAGA TTACTCCGAA 
GCAGAACAGT CAGATGAGCA GCTTTACCAA GAAATAACAA AGGCTAATGT TATATGCATC 
GTCTATTCAG TGAACAATAA GAAATCAATC GAAAAAGTGA CGAGTCATTG GATTCCACTC 
ATCAATGAAA GAACAGATAA GGACAGCAGA GTGCCGCTAA TCCTGGTGGG CAATAAATCT 
GACCTGGTGG AGCACAGCAG CATGGAGACC ATCCTGCCCA TCATGAACCA GTACTCAGAG 
ATTGAGACCT GTGTGGAGTG TTCTGCCAAA AACCTGAAGA ATATCTCTGA GCTGTTTTAT 
TATGCCCAGA AGGCTGTTCT TCACCCCACA GGACCTCTGT ACTCGCCAGA AGAGAAAGAG 
ATGAAGCCGT CTTGCATCAA AGCACTCACT CGCATTTTCA AAATATCAGA CCTGGATAAT 
GATGGCATTC TGAATGATAA CGAGCTTAAT TTCTTCCAGA GAACATGTTT TAACATACCA 
CTGGCACCTC AAGCCCTGGA GGACGTCAAG AATGTCGTGC GGAAAAATAT GACCGATGGA 
GTCAAAGACA ACGGCCTTAC ACTGAAGGGT TTCCTGTTTC TTCACACACT CTTCATTCAG 
AGGGGGAGGC ATGAGACCAC ATGGACCGTG CTTAGGAGGT TTGGTTATGA TGATGATCTG 
GAGCTTACAC AAGAATACCT GTTTCCACTG TTTAAAATAC CTCCAGACTG CACAACAGAG 
CTGAATCACA ATGCCTACCT CTTCCTCCAG AGTGTGTTTG ACAAACATGA CAAAGATCGA 
GACTGTGCCC TGTCTCCAGA TGAGCTGAAG GATTTGTTCA AGGTGTTTCC CTACATGCCC 
TGGGGTCCCG ATGTCAACAA CACAGTCTGC ACCAATGAGC AAGGCTGGAT CACTTACCAA 
GGCTACCTTT CCCAGTGGAC ACTTACAACA TACCTAGATG TGCAGCGGTG TTTGGAGTAC 
CTGGGATATC TCGGCTACTC TATTATTCAA GAGCAGGAGT CACAAGCAGC TGCCATCACA 
GTCACCAGGA ACAAGCGTAT TGACCTGCAG AAGAAGCAGA CCCAGCGCAG TGTTTTCCGA 
TGTAATGTCC TCGGGGCTCG AGGCTGTGGT AAAAGTGGCT TTCTGCAGGC TTTCCTGGGC 
AGGAACCTTG TGCGCCAGAA GAGGATAAGA GAAGACCACA AGTCTTATTA TGCCATTAGC 
ACTACTTATG TTTATGGACA GGAGAAATAT CTGCTCCTTC ACGAGGTGCT TCCAGACGTG 
GAGTTTCTTT CTGAAGCTGA TCTGGCTTGT GATGTTGTTT GTCTGGTTTA TGACATCAGC 
AACCCACGCT CATTTGAATA CTGCGCTAAA GTCTACAAGA AACACTTCAT GGACAGCAAG 
ACCCCATGTG TGATCATTGC TGCCAAGTCT GACCTGCATG AAGCCCGTCA GTACTACAGT 
CTCTCGCCGT TGGACTTCTG CCGAAAACAC AAGCTTCACC CGCCGCAGCT GTTCACCTGC 
AACACCACCG AAGCTCCGAG CAAGGACCTC TACACCAAAC TCACCACTAT GGCCATGTAC 
CCCCACGCCA AGTTACGCTG CATGTGCGGC TGCAACAGGT GCACCTATTG CATCTGTCAC 
AACCTCCTGA ACTCTGAGCT GGTGCGCTCG GTAAAGGCCA AATTCTCCAG CGCTGTTCTC 
AACAGGTGTT CCTTTTCATT TGTTTGGCTG GCCCTTTTCC TCTTTGCTTT GCATCTTTTC 
ACATGCTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHOT1A ( XM_009299425.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009297700.1 Danio rerio ras homolog family member T1a (rhot1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39897
Clone ID Related Accession (Same CDS sequence)	XM_009299425.3 , XM_009299425.2
Accession Version	XM_009299425.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mitochondrial Rho GTPase 1-A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299425.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGG ACGTGAGGAT ATTACTGGTC GGAGAACCCA AAGTGGGGAA GACCTCTCTT 
ATCATGTCAC TCGTCAGCGA GGAATTTCCT GATGAGGTTC CTCCAAGAGC CGAAGAGATT 
ACTATTCCTG CAGACGTCAC ACCTGAGCGA GTGCCCACTC ATATAGTAGA TTACTCCGAA 
GCAGAACAGT CAGATGAGCA GCTTTACCAA GAAATAACAA AGGCTAATGT TATATGCATC 
GTCTATTCAG TGAACAATAA GAAATCAATC GAAAAAGTGA CGAGTCATTG GATTCCACTC 
ATCAATGAAA GAACAGATAA GGACAGCAGA GTGCCGCTAA TCCTGGTGGG CAATAAATCT 
GACCTGGTGG AGCACAGCAG CATGGAGACC ATCCTGCCCA TCATGAACCA GTACTCAGAG 
ATTGAGACCT GTGTGGAGTG TTCTGCCAAA AACCTGAAGA ATATCTCTGA GCTGTTTTAT 
TATGCCCAGA AGGCTGTTCT TCACCCCACA GGACCTCTGT ACTCGCCAGA AGAGAAAGAG 
ATGAAGCCGT CTTGCATCAA AGCACTCACT CGCATTTTCA AAATATCAGA CCTGGATAAT 
GATGGCATTC TGAATGATAA CGAGCTTAAT TTCTTCCAGA GAACATGTTT TAACATACCA 
CTGGCACCTC AAGCCCTGGA GGACGTCAAG AATGTCGTGC GGAAAAATAT GACCGATGGA 
GTCAAAGACA ACGGCCTTAC ACTGAAGGGT TTCCTGTTTC TTCACACACT CTTCATTCAG 
AGGGGGAGGC ATGAGACCAC ATGGACCGTG CTTAGGAGGT TTGGTTATGA TGATGATCTG 
GAGCTTACAC AAGAATACCT GTTTCCACTG TTTAAAATAC CTCCAGACTG CACAACAGAG 
CTGAATCACA ATGCCTACCT CTTCCTCCAG AGTGTGTTTG ACAAACATGA CAAAGATCGA 
GACTGTGCCC TGTCTCCAGA TGAGCTGAAG GATTTGTTCA AGGTGTTTCC CTACATGCCC 
TGGGGTCCCG ATGTCAACAA CACAGTCTGC ACCAATGAGC AAGGCTGGAT CACTTACCAA 
GGCTACCTTT CCCAGTGGAC ACTTACAACA TACCTAGATG TGCAGCGGTG TTTGGAGTAC 
CTGGGATATC TCGGCTACTC TATTATTCAA GAGCAGGAGT CACAAGCAGC TGCCATCACA 
GTCACCAGGA ACAAGCGTAT TGACCTGCAG AAGAAGCAGA CCCAGCGCAG TGTTTTCCGA 
TGTAATGTCC TCGGGGCTCG AGGCTGTGGT AAAAGTGGCT TTCTGCAGGC TTTCCTGGGC 
AGGAACCTTG TGCGCCAGAA GAGGATAAGA GAAGACCACA AGTCTTATTA TGCCATTAGC 
ACTACTTATG TTTATGGACA GGAGAAATAT CTGCTCCTTC ACGAGGTGCT TCCAGACGTG 
GAGTTTCTTT CTGAAGCTGA TCTGGCTTGT GATGTTGTTT GTCTGGTTTA TGACATCAGC 
AACCCACGCT CATTTGAATA CTGCGCTAAA GTCTACAAGA AACACTTCAT GGACAGCAAG 
ACCCCATGTG TGATCATTGC TGCCAAGTCT GACCTGCATG AAGCCCGTCA GTACTACAGT 
CTCTCGCCGT TGGACTTCTG CCGAAAACAC AAGCTTCACC CGCCGCAGCT GTTCACCTGC 
AACACCACCG AAGCTCCGAG CAAGGACCTC TACACCAAAC TCACCACTAT GGCCATGTAC 
CCCCACGCCA AGTTACGCTG CATGTGCGGC TGCAACAGGT GCACCTATTG CATCTGTCAC 
AACCTCCTGA ACTCTGAGCT GGTGCGCTCG GTAAAGGCCA AATTCTCCAG CGCTGTTCTC 
AACAGGTTGA GACCTTTTAT ACAAGATCTG GGCACATTTC TGTTGGCTGA TGAGGAGTCC 
AGCCTCACCC ATCAGGTTCA TGCAGTCAGC TTTGTTGAGG ACTCGGTCTT CATGGAGTCG 
TCTGTCGACC CGTTTGTGCC CGCTTGTAAC AGACACATGA CCCAAGCTGA CCTGAAAAAC 
TCTACATTTT GGCTGAGGGC GAGCGTCGGT GCCACTGTCT TTGCTGTTCT GGGCTTTGCC 
ATGTACAAAG CTCTCCTCAA GCAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297700.1
CDS	335..2464
Translation	MRKDVRILLVGEPKVGKTSLIMSLVSEEFPDEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLYQEITKANVICIVYSVNNKKSIEKVTSHWIPLINERTDKDSRVPLILVGNKSDLVEHSSMETILPIMNQYSEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYSPEEKEMKPSCIKALTRIFKISDLDNDGILNDNELNFFQRTCFNIPLAPQALEDVKNVVRKNMTDGVKDNGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLELTQEYLFPLFKIPPDCTTELNHNAYLFLQSVFDKHDKDRDCALSPDELKDLFKVFPYMPWGPDVNNTVCTNEQGWITYQGYLSQWTLTTYLDVQRCLEYLGYLGYSIIQEQESQAAAITVTRNKRIDLQKKQTQRSVFRCNVLGARGCGKSGFLQAFLGRNLVRQKRIREDHKSYYAISTTYVYGQEKYLLLHEVLPDVEFLSEADLACDVVCLVYDISNPRSFEYCAKVYKKHFMDSKTPCVIIAAKSDLHEARQYYSLSPLDFCRKHKLHPPQLFTCNTTEAPSKDLYTKLTTMAMYPHAKLRCMCGCNRCTYCICHNLLNSELVRSVKAKFSSAVLNRLRPFIQDLGTFLLADEESSLTHQVHAVSFVEDSVFMESSVDPFVPACNRHMTQADLKNSTFWLRASVGATVFAVLGFAMYKALLKQR

Target ORF information:

RefSeq Version	XM_009299425.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ras homolog family member T1a (rhot1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299425.3

ORF Insert Sequence:

ATGAGAAAGG ACGTGAGGAT ATTACTGGTC GGAGAACCCA AAGTGGGGAA GACCTCTCTT 
ATCATGTCAC TCGTCAGCGA GGAATTTCCT GATGAGGTTC CTCCAAGAGC CGAAGAGATT 
ACTATTCCTG CAGACGTCAC ACCTGAGCGA GTGCCCACTC ATATAGTAGA TTACTCCGAA 
GCAGAACAGT CAGATGAGCA GCTTTACCAA GAAATAACAA AGGCTAATGT TATATGCATC 
GTCTATTCAG TGAACAATAA GAAATCAATC GAAAAAGTGA CGAGTCATTG GATTCCACTC 
ATCAATGAAA GAACAGATAA GGACAGCAGA GTGCCGCTAA TCCTGGTGGG CAATAAATCT 
GACCTGGTGG AGCACAGCAG CATGGAGACC ATCCTGCCCA TCATGAACCA GTACTCAGAG 
ATTGAGACCT GTGTGGAGTG TTCTGCCAAA AACCTGAAGA ATATCTCTGA GCTGTTTTAT 
TATGCCCAGA AGGCTGTTCT TCACCCCACA GGACCTCTGT ACTCGCCAGA AGAGAAAGAG 
ATGAAGCCGT CTTGCATCAA AGCACTCACT CGCATTTTCA AAATATCAGA CCTGGATAAT 
GATGGCATTC TGAATGATAA CGAGCTTAAT TTCTTCCAGA GAACATGTTT TAACATACCA 
CTGGCACCTC AAGCCCTGGA GGACGTCAAG AATGTCGTGC GGAAAAATAT GACCGATGGA 
GTCAAAGACA ACGGCCTTAC ACTGAAGGGT TTCCTGTTTC TTCACACACT CTTCATTCAG 
AGGGGGAGGC ATGAGACCAC ATGGACCGTG CTTAGGAGGT TTGGTTATGA TGATGATCTG 
GAGCTTACAC AAGAATACCT GTTTCCACTG TTTAAAATAC CTCCAGACTG CACAACAGAG 
CTGAATCACA ATGCCTACCT CTTCCTCCAG AGTGTGTTTG ACAAACATGA CAAAGATCGA 
GACTGTGCCC TGTCTCCAGA TGAGCTGAAG GATTTGTTCA AGGTGTTTCC CTACATGCCC 
TGGGGTCCCG ATGTCAACAA CACAGTCTGC ACCAATGAGC AAGGCTGGAT CACTTACCAA 
GGCTACCTTT CCCAGTGGAC ACTTACAACA TACCTAGATG TGCAGCGGTG TTTGGAGTAC 
CTGGGATATC TCGGCTACTC TATTATTCAA GAGCAGGAGT CACAAGCAGC TGCCATCACA 
GTCACCAGGA ACAAGCGTAT TGACCTGCAG AAGAAGCAGA CCCAGCGCAG TGTTTTCCGA 
TGTAATGTCC TCGGGGCTCG AGGCTGTGGT AAAAGTGGCT TTCTGCAGGC TTTCCTGGGC 
AGGAACCTTG TGCGCCAGAA GAGGATAAGA GAAGACCACA AGTCTTATTA TGCCATTAGC 
ACTACTTATG TTTATGGACA GGAGAAATAT CTGCTCCTTC ACGAGGTGCT TCCAGACGTG 
GAGTTTCTTT CTGAAGCTGA TCTGGCTTGT GATGTTGTTT GTCTGGTTTA TGACATCAGC 
AACCCACGCT CATTTGAATA CTGCGCTAAA GTCTACAAGA AACACTTCAT GGACAGCAAG 
ACCCCATGTG TGATCATTGC TGCCAAGTCT GACCTGCATG AAGCCCGTCA GTACTACAGT 
CTCTCGCCGT TGGACTTCTG CCGAAAACAC AAGCTTCACC CGCCGCAGCT GTTCACCTGC 
AACACCACCG AAGCTCCGAG CAAGGACCTC TACACCAAAC TCACCACTAT GGCCATGTAC 
CCCCACGCCA AGTTACGCTG CATGTGCGGC TGCAACAGGT GCACCTATTG CATCTGTCAC 
AACCTCCTGA ACTCTGAGCT GGTGCGCTCG GTAAAGGCCA AATTCTCCAG CGCTGTTCTC 
AACAGGTTGA GACCTTTTAT ACAAGATCTG GGCACATTTC TGTTGGCTGA TGAGGAGTCC 
AGCCTCACCC ATCAGGTTCA TGCAGTCAGC TTTGTTGAGG ACTCGGTCTT CATGGAGTCG 
TCTGTCGACC CGTTTGTGCC CGCTTGTAAC AGACACATGA CCCAAGCTGA CCTGAAAAAC 
TCTACATTTT GGCTGAGGGC GAGCGTCGGT GCCACTGTCT TTGCTGTTCT GGGCTTTGCC 
ATGTACAAAG CTCTCCTCAA GCAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHOT1A ( XM_009299425.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009297700.1 Danio rerio ras homolog family member T1a (rhot1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39897
Clone ID Related Accession (Same CDS sequence)	XM_009299425.3 , XM_009299425.2
Accession Version	XM_009299425.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mitochondrial Rho GTPase 1-A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299425.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGG ACGTGAGGAT ATTACTGGTC GGAGAACCCA AAGTGGGGAA GACCTCTCTT 
ATCATGTCAC TCGTCAGCGA GGAATTTCCT GATGAGGTTC CTCCAAGAGC CGAAGAGATT 
ACTATTCCTG CAGACGTCAC ACCTGAGCGA GTGCCCACTC ATATAGTAGA TTACTCCGAA 
GCAGAACAGT CAGATGAGCA GCTTTACCAA GAAATAACAA AGGCTAATGT TATATGCATC 
GTCTATTCAG TGAACAATAA GAAATCAATC GAAAAAGTGA CGAGTCATTG GATTCCACTC 
ATCAATGAAA GAACAGATAA GGACAGCAGA GTGCCGCTAA TCCTGGTGGG CAATAAATCT 
GACCTGGTGG AGCACAGCAG CATGGAGACC ATCCTGCCCA TCATGAACCA GTACTCAGAG 
ATTGAGACCT GTGTGGAGTG TTCTGCCAAA AACCTGAAGA ATATCTCTGA GCTGTTTTAT 
TATGCCCAGA AGGCTGTTCT TCACCCCACA GGACCTCTGT ACTCGCCAGA AGAGAAAGAG 
ATGAAGCCGT CTTGCATCAA AGCACTCACT CGCATTTTCA AAATATCAGA CCTGGATAAT 
GATGGCATTC TGAATGATAA CGAGCTTAAT TTCTTCCAGA GAACATGTTT TAACATACCA 
CTGGCACCTC AAGCCCTGGA GGACGTCAAG AATGTCGTGC GGAAAAATAT GACCGATGGA 
GTCAAAGACA ACGGCCTTAC ACTGAAGGGT TTCCTGTTTC TTCACACACT CTTCATTCAG 
AGGGGGAGGC ATGAGACCAC ATGGACCGTG CTTAGGAGGT TTGGTTATGA TGATGATCTG 
GAGCTTACAC AAGAATACCT GTTTCCACTG TTTAAAATAC CTCCAGACTG CACAACAGAG 
CTGAATCACA ATGCCTACCT CTTCCTCCAG AGTGTGTTTG ACAAACATGA CAAAGATCGA 
GACTGTGCCC TGTCTCCAGA TGAGCTGAAG GATTTGTTCA AGGTGTTTCC CTACATGCCC 
TGGGGTCCCG ATGTCAACAA CACAGTCTGC ACCAATGAGC AAGGCTGGAT CACTTACCAA 
GGCTACCTTT CCCAGTGGAC ACTTACAACA TACCTAGATG TGCAGCGGTG TTTGGAGTAC 
CTGGGATATC TCGGCTACTC TATTATTCAA GAGCAGGAGT CACAAGCAGC TGCCATCACA 
GTCACCAGGA ACAAGCGTAT TGACCTGCAG AAGAAGCAGA CCCAGCGCAG TGTTTTCCGA 
TGTAATGTCC TCGGGGCTCG AGGCTGTGGT AAAAGTGGCT TTCTGCAGGC TTTCCTGGGC 
AGGAACCTTG TGCGCCAGAA GAGGATAAGA GAAGACCACA AGTCTTATTA TGCCATTAGC 
ACTACTTATG TTTATGGACA GGAGAAATAT CTGCTCCTTC ACGAGGTGCT TCCAGACGTG 
GAGTTTCTTT CTGAAGCTGA TCTGGCTTGT GATGTTGTTT GTCTGGTTTA TGACATCAGC 
AACCCACGCT CATTTGAATA CTGCGCTAAA GTCTACAAGA AACACTTCAT GGACAGCAAG 
ACCCCATGTG TGATCATTGC TGCCAAGTCT GACCTGCATG AAGCCCGTCA GTACTACAGT 
CTCTCGCCGT TGGACTTCTG CCGAAAACAC AAGCTTCACC CGCCGCAGCT GTTCACCTGC 
AACACCACCG AAGCTCCGAG CAAGGACCTC TACACCAAAC TCACCACTAT GGCCATGTAC 
CCCCACGCCA AGTTACGCTG CATGTGCGGC TGCAACAGGT GCACCTATTG CATCTGTCAC 
AACCTCCTGA ACTCTGAGCT GGTGCGCTCG GTAAAGGCCA AATTCTCCAG CGCTGTTCTC 
AACAGGTTGA GACCTTTTAT ACAAGATCTG GGCACATTTC TGTTGGCTGA TGAGGAGTCC 
AGCCTCACCC ATCAGGTTCA TGCAGTCAGC TTTGTTGAGG ACTCGGTCTT CATGGAGTCG 
TCTGTCGACC CGTTTGTGCC CGCTTGTAAC AGACACATGA CCCAAGCTGA CCTGAAAAAC 
TCTACATTTT GGCTGAGGGC GAGCGTCGGT GCCACTGTCT TTGCTGTTCT GGGCTTTGCC 
ATGTACAAAG CTCTCCTCAA GCAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297700.1
CDS	331..2460
Translation	MRKDVRILLVGEPKVGKTSLIMSLVSEEFPDEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLYQEITKANVICIVYSVNNKKSIEKVTSHWIPLINERTDKDSRVPLILVGNKSDLVEHSSMETILPIMNQYSEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYSPEEKEMKPSCIKALTRIFKISDLDNDGILNDNELNFFQRTCFNIPLAPQALEDVKNVVRKNMTDGVKDNGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLELTQEYLFPLFKIPPDCTTELNHNAYLFLQSVFDKHDKDRDCALSPDELKDLFKVFPYMPWGPDVNNTVCTNEQGWITYQGYLSQWTLTTYLDVQRCLEYLGYLGYSIIQEQESQAAAITVTRNKRIDLQKKQTQRSVFRCNVLGARGCGKSGFLQAFLGRNLVRQKRIREDHKSYYAISTTYVYGQEKYLLLHEVLPDVEFLSEADLACDVVCLVYDISNPRSFEYCAKVYKKHFMDSKTPCVIIAAKSDLHEARQYYSLSPLDFCRKHKLHPPQLFTCNTTEAPSKDLYTKLTTMAMYPHAKLRCMCGCNRCTYCICHNLLNSELVRSVKAKFSSAVLNRLRPFIQDLGTFLLADEESSLTHQVHAVSFVEDSVFMESSVDPFVPACNRHMTQADLKNSTFWLRASVGATVFAVLGFAMYKALLKQR

Target ORF information:

RefSeq Version	XM_009299425.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ras homolog family member T1a (rhot1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299425.2

ORF Insert Sequence:

ATGAGAAAGG ACGTGAGGAT ATTACTGGTC GGAGAACCCA AAGTGGGGAA GACCTCTCTT 
ATCATGTCAC TCGTCAGCGA GGAATTTCCT GATGAGGTTC CTCCAAGAGC CGAAGAGATT 
ACTATTCCTG CAGACGTCAC ACCTGAGCGA GTGCCCACTC ATATAGTAGA TTACTCCGAA 
GCAGAACAGT CAGATGAGCA GCTTTACCAA GAAATAACAA AGGCTAATGT TATATGCATC 
GTCTATTCAG TGAACAATAA GAAATCAATC GAAAAAGTGA CGAGTCATTG GATTCCACTC 
ATCAATGAAA GAACAGATAA GGACAGCAGA GTGCCGCTAA TCCTGGTGGG CAATAAATCT 
GACCTGGTGG AGCACAGCAG CATGGAGACC ATCCTGCCCA TCATGAACCA GTACTCAGAG 
ATTGAGACCT GTGTGGAGTG TTCTGCCAAA AACCTGAAGA ATATCTCTGA GCTGTTTTAT 
TATGCCCAGA AGGCTGTTCT TCACCCCACA GGACCTCTGT ACTCGCCAGA AGAGAAAGAG 
ATGAAGCCGT CTTGCATCAA AGCACTCACT CGCATTTTCA AAATATCAGA CCTGGATAAT 
GATGGCATTC TGAATGATAA CGAGCTTAAT TTCTTCCAGA GAACATGTTT TAACATACCA 
CTGGCACCTC AAGCCCTGGA GGACGTCAAG AATGTCGTGC GGAAAAATAT GACCGATGGA 
GTCAAAGACA ACGGCCTTAC ACTGAAGGGT TTCCTGTTTC TTCACACACT CTTCATTCAG 
AGGGGGAGGC ATGAGACCAC ATGGACCGTG CTTAGGAGGT TTGGTTATGA TGATGATCTG 
GAGCTTACAC AAGAATACCT GTTTCCACTG TTTAAAATAC CTCCAGACTG CACAACAGAG 
CTGAATCACA ATGCCTACCT CTTCCTCCAG AGTGTGTTTG ACAAACATGA CAAAGATCGA 
GACTGTGCCC TGTCTCCAGA TGAGCTGAAG GATTTGTTCA AGGTGTTTCC CTACATGCCC 
TGGGGTCCCG ATGTCAACAA CACAGTCTGC ACCAATGAGC AAGGCTGGAT CACTTACCAA 
GGCTACCTTT CCCAGTGGAC ACTTACAACA TACCTAGATG TGCAGCGGTG TTTGGAGTAC 
CTGGGATATC TCGGCTACTC TATTATTCAA GAGCAGGAGT CACAAGCAGC TGCCATCACA 
GTCACCAGGA ACAAGCGTAT TGACCTGCAG AAGAAGCAGA CCCAGCGCAG TGTTTTCCGA 
TGTAATGTCC TCGGGGCTCG AGGCTGTGGT AAAAGTGGCT TTCTGCAGGC TTTCCTGGGC 
AGGAACCTTG TGCGCCAGAA GAGGATAAGA GAAGACCACA AGTCTTATTA TGCCATTAGC 
ACTACTTATG TTTATGGACA GGAGAAATAT CTGCTCCTTC ACGAGGTGCT TCCAGACGTG 
GAGTTTCTTT CTGAAGCTGA TCTGGCTTGT GATGTTGTTT GTCTGGTTTA TGACATCAGC 
AACCCACGCT CATTTGAATA CTGCGCTAAA GTCTACAAGA AACACTTCAT GGACAGCAAG 
ACCCCATGTG TGATCATTGC TGCCAAGTCT GACCTGCATG AAGCCCGTCA GTACTACAGT 
CTCTCGCCGT TGGACTTCTG CCGAAAACAC AAGCTTCACC CGCCGCAGCT GTTCACCTGC 
AACACCACCG AAGCTCCGAG CAAGGACCTC TACACCAAAC TCACCACTAT GGCCATGTAC 
CCCCACGCCA AGTTACGCTG CATGTGCGGC TGCAACAGGT GCACCTATTG CATCTGTCAC 
AACCTCCTGA ACTCTGAGCT GGTGCGCTCG GTAAAGGCCA AATTCTCCAG CGCTGTTCTC 
AACAGGTTGA GACCTTTTAT ACAAGATCTG GGCACATTTC TGTTGGCTGA TGAGGAGTCC 
AGCCTCACCC ATCAGGTTCA TGCAGTCAGC TTTGTTGAGG ACTCGGTCTT CATGGAGTCG 
TCTGTCGACC CGTTTGTGCC CGCTTGTAAC AGACACATGA CCCAAGCTGA CCTGAAAAAC 
TCTACATTTT GGCTGAGGGC GAGCGTCGGT GCCACTGTCT TTGCTGTTCT GGGCTTTGCC 
ATGTACAAAG CTCTCCTCAA GCAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHOT1A ( XM_017353420.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208909.1 Danio rerio ras homolog family member T1a (rhot1a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa58023
Clone ID Related Accession (Same CDS sequence)	XM_017353420.1
Accession Version	XM_017353420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mitochondrial Rho GTPase 1-A isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGG ACGTGAGGAT ATTACTGGTC GGAGAACCCA AAGTGGGGAA GACCTCTCTT 
ATCATGTCAC TCGTCAGCGA GGAATTTCCT GATGAGGTTC CTCCAAGAGC CGAAGAGATT 
ACTATTCCTG CAGACGTCAC ACCTGAGCGA GTGCCCACTC ATATAGTAGA TTACTCCGAA 
GCAGAACAGT CAGATGAGCA GCTTTACCAA GAAATAACAA AGGCTAATGT TATATGCATC 
GTCTATTCAG TGAACAATAA GAAATCAATC GAAAAAGTGA CGAGTCATTG GATTCCACTC 
ATCAATGAAA GAACAGATAA GGACAGCAGA GTGCCGCTAA TCCTGGTGGG CAATAAATCT 
GACCTGGTGG AGCACAGCAG CATGGAGACC ATCCTGCCCA TCATGAACCA GTACTCAGAG 
ATTGAGACCT GTGTGGAGTG TTCTGCCAAA AACCTGAAGA ATATCTCTGA GCTGTTTTAT 
TATGCCCAGA AGGCTGTTCT TCACCCCACA GGACCTCTGT ACTCGCCAGA AGAGAAAGAG 
ATGAAGCCGT CTTGCATCAA AGCACTCACT CGCATTTTCA AAATATCAGA CCTGGATAAT 
GATGGCATTC TGAATGATAA CGAGCTTAAT TTCTTCCAGA GAACATGTTT TAACATACCA 
CTGGCACCTC AAGCCCTGGA GGACGTCAAG AATGTCGTGC GGAAAAATAT GACCGATGGA 
GTCAAAGACA ACGGCCTTAC ACTGAAGGGT TTCCTGTTTC TTCACACACT CTTCATTCAG 
AGGGGGAGGC ATGAGACCAC ATGGACCGTG CTTAGGAGGT TTGGTTATGA TGATGATCTG 
GAGCTTACAC AAGAATACCT GTTTCCACTG TTTAAAATAC CTCCAGACTG CACAACAGAG 
CTGAATCACA ATGCCTACCT CTTCCTCCAG AGTGTGTTTG ACAAACATGA CAAAGATCGA 
GACTGTGCCC TGTCTCCAGA TGAGCTGAAG GATTTGTTCA AGGTGTTTCC CTACATGCCC 
TGGGGTCCCG ATGTCAACAA CACAGTCTGC ACCAATGAGC AAGGCTGGAT CACTTACCAA 
GGCTACCTTT CCCAGTGGAC ACTTACAACA TACCTAGATG TGCAGCGGTG TTTGGAGTAC 
CTGGGATATC TCGGCTACTC TATTATTCAA GAGCAGGAGT CACAAGCAGC TGCCATCACA 
GTCACCAGGA ACAAGCGTAT TGACCTGCAG AAGAAGCAGA CCCAGCGCAG TGTTTTCCGA 
TGTAATGTCC TCGGGGCTCG AGGCTGTGGT AAAAGTGGCT TTCTGCAGGC TTTCCTGGGC 
AGGAACCTTG TGCGCCAGAA GAGGATAAGA GAAGACCACA AGTCTTATTA TGCCATTAGC 
ACTACTTATG TTTATGGACA GGAGAAATAT CTGCTCCTTC ACGAGGTGCT TCCAGACGTG 
GAGTTTCTTT CTGAAGCTGA TCTGGCTTGT GATGTTGTTT GTCTGGTTTA TGACATCAGC 
AACCCACGCT CATTTGAATA CTGCGCTAAA GTCTACAAGA AACACTTCAT GGACAGCAAG 
ACCCCATGTG TGATCATTGC TGCCAAGTCT GACCTGCATG AAGCCCGTCA GTACTACAGT 
CTCTCGCCGT TGGACTTCTG CCGAAAACAC AAGCTTCACC CGCCGCAGCT GTTCACCTGC 
AACACCACCG AAGCTCCGAG CAAGGACCTC TACACCAAAC TCACCACTAT GGCCATGTAC 
CCGGCTGCAC AATTTGGCTC AAAATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208909.1
CDS	331..2100
Translation	MRKDVRILLVGEPKVGKTSLIMSLVSEEFPDEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLYQEITKANVICIVYSVNNKKSIEKVTSHWIPLINERTDKDSRVPLILVGNKSDLVEHSSMETILPIMNQYSEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYSPEEKEMKPSCIKALTRIFKISDLDNDGILNDNELNFFQRTCFNIPLAPQALEDVKNVVRKNMTDGVKDNGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLELTQEYLFPLFKIPPDCTTELNHNAYLFLQSVFDKHDKDRDCALSPDELKDLFKVFPYMPWGPDVNNTVCTNEQGWITYQGYLSQWTLTTYLDVQRCLEYLGYLGYSIIQEQESQAAAITVTRNKRIDLQKKQTQRSVFRCNVLGARGCGKSGFLQAFLGRNLVRQKRIREDHKSYYAISTTYVYGQEKYLLLHEVLPDVEFLSEADLACDVVCLVYDISNPRSFEYCAKVYKKHFMDSKTPCVIIAAKSDLHEARQYYSLSPLDFCRKHKLHPPQLFTCNTTEAPSKDLYTKLTTMAMYPAAQFGSKL

Target ORF information:

RefSeq Version	XM_017353420.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ras homolog family member T1a (rhot1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353420.1

ORF Insert Sequence:

ATGAGAAAGG ACGTGAGGAT ATTACTGGTC GGAGAACCCA AAGTGGGGAA GACCTCTCTT 
ATCATGTCAC TCGTCAGCGA GGAATTTCCT GATGAGGTTC CTCCAAGAGC CGAAGAGATT 
ACTATTCCTG CAGACGTCAC ACCTGAGCGA GTGCCCACTC ATATAGTAGA TTACTCCGAA 
GCAGAACAGT CAGATGAGCA GCTTTACCAA GAAATAACAA AGGCTAATGT TATATGCATC 
GTCTATTCAG TGAACAATAA GAAATCAATC GAAAAAGTGA CGAGTCATTG GATTCCACTC 
ATCAATGAAA GAACAGATAA GGACAGCAGA GTGCCGCTAA TCCTGGTGGG CAATAAATCT 
GACCTGGTGG AGCACAGCAG CATGGAGACC ATCCTGCCCA TCATGAACCA GTACTCAGAG 
ATTGAGACCT GTGTGGAGTG TTCTGCCAAA AACCTGAAGA ATATCTCTGA GCTGTTTTAT 
TATGCCCAGA AGGCTGTTCT TCACCCCACA GGACCTCTGT ACTCGCCAGA AGAGAAAGAG 
ATGAAGCCGT CTTGCATCAA AGCACTCACT CGCATTTTCA AAATATCAGA CCTGGATAAT 
GATGGCATTC TGAATGATAA CGAGCTTAAT TTCTTCCAGA GAACATGTTT TAACATACCA 
CTGGCACCTC AAGCCCTGGA GGACGTCAAG AATGTCGTGC GGAAAAATAT GACCGATGGA 
GTCAAAGACA ACGGCCTTAC ACTGAAGGGT TTCCTGTTTC TTCACACACT CTTCATTCAG 
AGGGGGAGGC ATGAGACCAC ATGGACCGTG CTTAGGAGGT TTGGTTATGA TGATGATCTG 
GAGCTTACAC AAGAATACCT GTTTCCACTG TTTAAAATAC CTCCAGACTG CACAACAGAG 
CTGAATCACA ATGCCTACCT CTTCCTCCAG AGTGTGTTTG ACAAACATGA CAAAGATCGA 
GACTGTGCCC TGTCTCCAGA TGAGCTGAAG GATTTGTTCA AGGTGTTTCC CTACATGCCC 
TGGGGTCCCG ATGTCAACAA CACAGTCTGC ACCAATGAGC AAGGCTGGAT CACTTACCAA 
GGCTACCTTT CCCAGTGGAC ACTTACAACA TACCTAGATG TGCAGCGGTG TTTGGAGTAC 
CTGGGATATC TCGGCTACTC TATTATTCAA GAGCAGGAGT CACAAGCAGC TGCCATCACA 
GTCACCAGGA ACAAGCGTAT TGACCTGCAG AAGAAGCAGA CCCAGCGCAG TGTTTTCCGA 
TGTAATGTCC TCGGGGCTCG AGGCTGTGGT AAAAGTGGCT TTCTGCAGGC TTTCCTGGGC 
AGGAACCTTG TGCGCCAGAA GAGGATAAGA GAAGACCACA AGTCTTATTA TGCCATTAGC 
ACTACTTATG TTTATGGACA GGAGAAATAT CTGCTCCTTC ACGAGGTGCT TCCAGACGTG 
GAGTTTCTTT CTGAAGCTGA TCTGGCTTGT GATGTTGTTT GTCTGGTTTA TGACATCAGC 
AACCCACGCT CATTTGAATA CTGCGCTAAA GTCTACAAGA AACACTTCAT GGACAGCAAG 
ACCCCATGTG TGATCATTGC TGCCAAGTCT GACCTGCATG AAGCCCGTCA GTACTACAGT 
CTCTCGCCGT TGGACTTCTG CCGAAAACAC AAGCTTCACC CGCCGCAGCT GTTCACCTGC 
AACACCACCG AAGCTCCGAG CAAGGACCTC TACACCAAAC TCACCACTAT GGCCATGTAC 
CCGGCTGCAC AATTTGGCTC AAAATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Targeted Gene Expression in Zebrafish Exposed to Chlorpyrifos-Oxon Confirms Phenotype-Specific Mechanisms Leading to Adverse Outcomes.
Bulletin of environmental contamination and toxicology96(6)707-13(2016 Jun)
Garcia-Reyero N,Escalon L,Prats E,Faria M,Soares AM,Rald?a D

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Characterization of the three zebrafish orthologs of the mitochondrial GTPase Miro/Rhot.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology191126-34(2016 Jan)
Hollister BM,Oonk KA,Weiser DC,Walsh S

Xmrk, kras and myc transgenic zebrafish liver cancer models share molecular signatures with subsets of human hepatocellular carcinoma.
PloS one9(3)e91179(2014)
Zheng W,Li Z,Nguyen AT,Li C,Emelyanov A,Gong Z

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RHOT1A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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