Pole3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pole3
Entrez Gene ID	59001
Full Name	polymerase (DNA directed), epsilon 3 (p17 subunit)
Synonyms	1810034K18Rik,YBL1
General protein information	Preferred Names polymerase (DNA directed), epsilon 3 (p17 subunit) Names DNA polymerase epsilon subunit 3 DNA polymerase II subunit 3 DNA polymerase epsilon subunit p17 NF-YB-like protein YB-like protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_021498.2	NP_067473.2	DNA polymerase epsilon subunit 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	POLE3	NP_059139.3
	POLE3	XP_001101300.1
Canis lupus familiaris (dog)	POLE3	XP_855374.2
Gallus gallus (chicken)	POLE3	NP_001020525.2
Danio rerio (zebrafish)	pole3	NP_957095.1
Drosophila melanogaster (fruit fly)	Chrac-14	NP_476646.2
Bos taurus (cattle)	POLE3	NP_001029562.1
Rattus norvegicus (Norway rat)	Pole3	NP_001007653.1
Rattus norvegicus (Norway rat)	LOC100362333	XP_002729875.1
Pan troglodytes (chimpanzee)	POLE3	NP_001233445.1
Mus musculus (house mouse)	Pole3	NP_067473.2
Xenopus tropicalis (tropical clawed frog)	pole3	NP_001017264.1

Gene Ontology
Bibliography

Related articles in PubMed

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
Guo G, Huss M, Tong GQ, Wang C, Li Sun L, Clarke ND, Robson P
Developmental cell18(4)675-85(2010 Apr)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Identification of candidate lung cancer susceptibility genes in mouse using oligonucleotide arrays.
Lemon WJ, Bernert H, Sun H, Wang Y, You M
Journal of medical genetics39(9)644-55(2002 Sep)

Cloning and characterization of the histone-fold proteins YBL1 and YCL1.
Bolognese F, Imbriano C, Caretti G, Mantovani R
Nucleic acids research28(19)3830-8(2000 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pole3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pole3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu12415	NM_021498.2 Latest version!	Mus musculus polymerase (DNA directed), epsilon 3 (p17 subunit) (Pole3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pole3 ( NM_021498.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067473.2 Mus musculus polymerase (DNA directed), epsilon 3 (p17 subunit) (Pole3), mRNA.

Price & Availability↑

CloneID	OMu12415
Clone ID Related Accession (Same CDS sequence)	NM_021498.2
Accession Version	NM_021498.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	DNA polymerase epsilon subunit 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC024996.1. On Jun 19, 2003 this sequence version replaced NM_021498.1. ##Evidence-Data-START## Transcript exon combination :: BC024996.1, BI455203.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGA GGCCCGAGGA CCTAAATCTG CCCAACGCCG TCATCACCAG GATCATCAAG 
GAGGCGCTCC CGGACGGTGT CAACATCTCC AAGGAGGCCC GAAGCGCCAT CTCCCGCGCC 
GCCAGTGTCT TCGTTTTGTA TGCCACATCC TGTGCCAATA ACTTCGCAAT GAAAGGAAAG 
CGCAAGACTC TCAATGCCAG TGACGTGCTG TCAGCCATGG AAGAGATGGA ATTCCAGCGA 
TTCATAACAC CATTGAAAGA AGCTCTAGAA GCGTACAGGC GGGAGCAGAA AGGCAAGAAG 
GAGGCTTCGG AGCAAAAGAA GAAGGACAAA GACAAAAAGG ATTCCGAAGA GCAAGACAAA 
AGCAGAGAGG AGGAGGAGGA GGATGAAGAG AGACTGGACG AGGACGACCA GAACGAAGAG 
GAGGAAATAG ACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067473.2
CDS	19..456
Translation	MAERPEDLNLPNAVITRIIKEALPDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTLNASDVLSAMEEMEFQRFITPLKEALEAYRREQKGKKEASEQKKKDKDKKDSEEQDKSREEEEEDEERLDEDDQNEEEEIDN

Target ORF information:

RefSeq Version	NM_021498.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), epsilon 3 (p17 subunit) (Pole3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021498.2

ORF Insert Sequence:

ATGGCGGAGA GGCCCGAGGA CCTAAATCTG CCCAACGCCG TCATCACCAG GATCATCAAG 
GAGGCGCTCC CGGACGGTGT CAACATCTCC AAGGAGGCCC GAAGCGCCAT CTCCCGCGCC 
GCCAGTGTCT TCGTTTTGTA TGCCACATCC TGTGCCAATA ACTTCGCAAT GAAAGGAAAG 
CGCAAGACTC TCAATGCCAG TGACGTGCTG TCAGCCATGG AAGAGATGGA ATTCCAGCGA 
TTCATAACAC CATTGAAAGA AGCTCTAGAA GCGTACAGGC GGGAGCAGAA AGGCAAGAAG 
GAGGCTTCGG AGCAAAAGAA GAAGGACAAA GACAAAAAGG ATTCCGAAGA GCAAGACAAA 
AGCAGAGAGG AGGAGGAGGA GGATGAAGAG AGACTGGACG AGGACGACCA GAACGAAGAG 
GAGGAAATAG ACAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
Developmental cell18(4)675-85(2010 Apr)
Guo G,Huss M,Tong GQ,Wang C,Li Sun L,Clarke ND,Robson P

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

Identification of candidate lung cancer susceptibility genes in mouse using oligonucleotide arrays.
Journal of medical genetics39(9)644-55(2002 Sep)
Lemon WJ,Bernert H,Sun H,Wang Y,You M

Cloning and characterization of the histone-fold proteins YBL1 and YCL1.
Nucleic acids research28(19)3830-8(2000 Oct)
Bolognese F,Imbriano C,Caretti G,Mantovani R