Crtap cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Crtap
Entrez Gene ID	56693
Full Name	cartilage associated protein
Synonyms	5730529N23Rik,CASP,Leprel3,P3h5
General protein information	Preferred Names cartilage associated protein Names cartilage-associated protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_019922.2	NP_064306.2	cartilage-associated protein precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CRTAP	XP_542696.2
Mus musculus (house mouse)	Crtap	NP_064306.2
Rattus norvegicus (Norway rat)	LOC102555086	XP_006244084.1
Danio rerio (zebrafish)	crtap	NP_001001406.1
Pan troglodytes (chimpanzee)	CRTAP	XP_001168451.1
Xenopus tropicalis (tropical clawed frog)	crtap	NP_001016647.1
Bos taurus (cattle)	LOC781218	XP_005219926.1
Homo sapiens (human)	CRTAP	NP_006362.1
Bos taurus (cattle)	CRTAP	NP_001192974.1
Gallus gallus (chicken)	CRTAP	NP_990431.1
Caenorhabditis elegans (roundworm)	CELE_Y73F8A.26	NP_502862.1
	CRTAP	XP_001099429.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003898	XP_001687842.2

Gene Ontology
Bibliography

Related articles in PubMed

Excessive transforming growth factor-? signaling is a common mechanism in osteogenesis imperfecta.
Grafe I, Yang T, Alexander S, Homan EP, Lietman C, Jiang MM, Bertin T, Munivez E, Chen Y, Dawson B, Ishikawa Y, Weis MA, Sampath TK, Ambrose C, Eyre D, B?chinger HP, Lee B
Nature medicine20(6)670-5(2014 Jun)

Differential effects of collagen prolyl 3-hydroxylation on skeletal tissues.
Homan EP, Lietman C, Grafe I, Lennington J, Morello R, Napierala D, Jiang MM, Munivez EM, Dawson B, Bertin TK, Chen Y, Lua R, Lichtarge O, Hicks J, Weis MA, Eyre D, Lee BH
PLoS genetics10(1)e1004121(2014 Jan)

An additional function of the rough endoplasmic reticulum protein complex prolyl 3-hydroxylase 1?cartilage-associated protein?cyclophilin B: the CXXXC motif reveals disulfide isomerase activity in vitro.
Ishikawa Y, B?chinger HP
The Journal of biological chemistry288(44)31437-46(2013 Nov)

Generalized connective tissue disease in Crtap-/- mouse.
Baldridge D, Lennington J, Weis M, Homan EP, Jiang MM, Munivez E, Keene DR, Hogue WR, Pyott S, Byers PH, Krakow D, Cohn DH, Eyre DR, Lee B, Morello R
PloS one5(5)e10560(2010 May)

CRTAP is required for prolyl 3- hydroxylation and mutations cause recessive osteogenesis imperfecta.
Morello R, Bertin TK, Chen Y, Hicks J, Tonachini L, Monticone M, Castagnola P, Rauch F, Glorieux FH, Vranka J, B?chinger HP, Pace JM, Schwarze U, Byers PH, Weis M, Fernandes RJ, Eyre DR, Yao Z, Boyce BF, Lee B
Cell127(2)291-304(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This substitution abolished P3h1 activity but retained ability to form a complex with Crtap and thus the collagen chaperone function.
Title: Differential effects of collagen prolyl 3-hydroxylation on skeletal tissues.

An additional function of the rough endoplasmic reticulum protein complex prolyl 3-hydroxylase 1.cartilage-associated protein.cyclophilin B: the CXXXC motif reveals disulfide isomerase activity in vitro.
Title: An additional function of the rough endoplasmic reticulum protein complex prolyl 3-hydroxylase 1?cartilage-associated protein?cyclophilin B: the CXXXC motif reveals disulfide isomerase activity in vitro.

In Crtap-deficient mice, the mineral content of the bone matrix is abnormally high; there are increased mineral densities at all measured sites for murine femora compared to control animals.
Title: CRTAP deficiency leads to abnormally high bone matrix mineralization in a murine model and in children with osteogenesis imperfecta type VII.

CRTAP mutations are associated with the clinical spectrum of recessive osteogenesis imperfecta, including the type II and VII forms
Title: CRTAP is required for prolyl 3- hydroxylation and mutations cause recessive osteogenesis imperfecta.

The following Crtap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Crtap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11807	NM_019922.2 Latest version!	Mus musculus cartilage associated protein (Crtap), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Crtap ( NM_019922.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_064306.2 Mus musculus cartilage associated protein (Crtap), mRNA.

Price & Availability↑

CloneID	OMu11807
Clone ID Related Accession (Same CDS sequence)	NM_019922.2
Accession Version	NM_019922.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	cartilage-associated protein precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ046463.1, BC049890.1 and BY699681.1. On Mar 21, 2009 this sequence version replaced NM_019922.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ006469.1, AK047506.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGGGGCCCC GCAGCCCTAC GGCCGCGTTG CTGGTGCTGC TGTGTGTCGG GTGCGCGCCG 
ACCCCCGGGC GCGGGCAGTA CGAGCGCTAC AGCTTCCGCA GCTTCCCGCG GGACGAGCTG 
ATGCCGCTCG AGTCGGCCTA CCGCCACGCG CTCGACCAGT ACAGCGGCGA GCACTGGGCC 
GAGAGCGTGG GCTACCTGGA GGTGAGCCTG CGGCTGCACC GTCTGCTGCG CGACAGCGAG 
GCCTTCTGCC ACCGCAACTG CAGCGCGGCC ACGCCAGCAC CCGCGCCCGC CGGCCCGGCC 
AGCCACGCCG AACTACGCCT CTTCGGCAGC GTGCTGCGCC GCGCGCAGTG CCTCAAGCGC 
TGCAAGCAGG GCCTGCCCGC CTTCCGCCAG TCGCAGCCCA GCCGCTCAGT GCTGGCGGAC 
TTTCAGCAGC GCGAGCCCTA CAAGTTTCTG CAGTTCGCCT ACTTCAAGGC CAATGACCTC 
CCGAAGGCCA TCGCTGCGGC TCACACCTAT CTCCTGAAGC ATCCAGATGA CGAGATGATG 
AAGAGAAACA TGGAGTATTA TAAGAGCTTG CCTGGAGCCG AGGACCACAT TAAAGACTTG 
GAAACCAAGT CGTACGAGAG CCTGTTTGTC CGTGCGGTGC GGGCCTACAA CGGGGAGAAC 
TGGAGAACGT CCATTTCCGA CATGGAGCTC GCGCTTCCCG ACTTCCTCAA GGCCTTCTAC 
GAGTGCCTGG CTGCCTGCGA GGGGTCGCGG GAGATCAAGG ACTTCAAGGA CTTCTACCTG 
TCCATAGCAG ATCACTATGT GGAAGTTCTG GAGTGTAAGA TTCGTTGTGA GGAGACCCTC 
ACCCCAGTCA TAGGAGGCTA TCCCGTGGAG AAATTTGTGG CGACCATGTA CCACTATTTA 
CAGTTTGCGT ATTACAAGTT GAATGATCTG AAGAATGCAG CCCCGTGTGC CGTCAGCTAC 
CTGCTCTTTG ACCAGAGTGA CAGGGTCATG CAACAGAACC TGGTGTACTA TCAGTACCAC 
CGGGACAAGT GGGGCCTCTC GGATGAGCAC TTCCAGCCCA GACCCGAAGC AGTTCAGTTC 
TTTAATGTGA CGACGCTCCA GAAGGAACTG TACGACTTCG CTCAGGAACA CCTAATGGAT 
GACGATGAGG GAGAGGTTGT GGAGTATGTG GACGACTTGT TGGAGACGGA AGAGTCTGCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064306.2
CDS	114..1316
Translation	MGPRSPTAALLVLLCVGCAPTPGRGQYERYSFRSFPRDELMPLESAYRHALDQYSGEHWAESVGYLEVSLRLHRLLRDSEAFCHRNCSAATPAPAPAGPASHAELRLFGSVLRRAQCLKRCKQGLPAFRQSQPSRSVLADFQQREPYKFLQFAYFKANDLPKAIAAAHTYLLKHPDDEMMKRNMEYYKSLPGAEDHIKDLETKSYESLFVRAVRAYNGENWRTSISDMELALPDFLKAFYECLAACEGSREIKDFKDFYLSIADHYVEVLECKIRCEETLTPVIGGYPVEKFVATMYHYLQFAYYKLNDLKNAAPCAVSYLLFDQSDRVMQQNLVYYQYHRDKWGLSDEHFQPRPEAVQFFNVTTLQKELYDFAQEHLMDDDEGEVVEYVDDLLETEESA

Target ORF information:

RefSeq Version	NM_019922.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cartilage associated protein (Crtap), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019922.2

ORF Insert Sequence:

ATGGGGCCCC GCAGCCCTAC GGCCGCGTTG CTGGTGCTGC TGTGTGTCGG GTGCGCGCCG 
ACCCCCGGGC GCGGGCAGTA CGAGCGCTAC AGCTTCCGCA GCTTCCCGCG GGACGAGCTG 
ATGCCGCTCG AGTCGGCCTA CCGCCACGCG CTCGACCAGT ACAGCGGCGA GCACTGGGCC 
GAGAGCGTGG GCTACCTGGA GGTGAGCCTG CGGCTGCACC GTCTGCTGCG CGACAGCGAG 
GCCTTCTGCC ACCGCAACTG CAGCGCGGCC ACGCCAGCAC CCGCGCCCGC CGGCCCGGCC 
AGCCACGCCG AACTACGCCT CTTCGGCAGC GTGCTGCGCC GCGCGCAGTG CCTCAAGCGC 
TGCAAGCAGG GCCTGCCCGC CTTCCGCCAG TCGCAGCCCA GCCGCTCAGT GCTGGCGGAC 
TTTCAGCAGC GCGAGCCCTA CAAGTTTCTG CAGTTCGCCT ACTTCAAGGC CAATGACCTC 
CCGAAGGCCA TCGCTGCGGC TCACACCTAT CTCCTGAAGC ATCCAGATGA CGAGATGATG 
AAGAGAAACA TGGAGTATTA TAAGAGCTTG CCTGGAGCCG AGGACCACAT TAAAGACTTG 
GAAACCAAGT CGTACGAGAG CCTGTTTGTC CGTGCGGTGC GGGCCTACAA CGGGGAGAAC 
TGGAGAACGT CCATTTCCGA CATGGAGCTC GCGCTTCCCG ACTTCCTCAA GGCCTTCTAC 
GAGTGCCTGG CTGCCTGCGA GGGGTCGCGG GAGATCAAGG ACTTCAAGGA CTTCTACCTG 
TCCATAGCAG ATCACTATGT GGAAGTTCTG GAGTGTAAGA TTCGTTGTGA GGAGACCCTC 
ACCCCAGTCA TAGGAGGCTA TCCCGTGGAG AAATTTGTGG CGACCATGTA CCACTATTTA 
CAGTTTGCGT ATTACAAGTT GAATGATCTG AAGAATGCAG CCCCGTGTGC CGTCAGCTAC 
CTGCTCTTTG ACCAGAGTGA CAGGGTCATG CAACAGAACC TGGTGTACTA TCAGTACCAC 
CGGGACAAGT GGGGCCTCTC GGATGAGCAC TTCCAGCCCA GACCCGAAGC AGTTCAGTTC 
TTTAATGTGA CGACGCTCCA GAAGGAACTG TACGACTTCG CTCAGGAACA CCTAATGGAT 
GACGATGAGG GAGAGGTTGT GGAGTATGTG GACGACTTGT TGGAGACGGA AGAGTCTGCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Excessive transforming growth factor-? signaling is a common mechanism in osteogenesis imperfecta.
Nature medicine20(6)670-5(2014 Jun)
Grafe I,Yang T,Alexander S,Homan EP,Lietman C,Jiang MM,Bertin T,Munivez E,Chen Y,Dawson B,Ishikawa Y,Weis MA,Sampath TK,Ambrose C,Eyre D,B?chinger HP,Lee B

Differential effects of collagen prolyl 3-hydroxylation on skeletal tissues.
PLoS genetics10(1)e1004121(2014 Jan)
Homan EP,Lietman C,Grafe I,Lennington J,Morello R,Napierala D,Jiang MM,Munivez EM,Dawson B,Bertin TK,Chen Y,Lua R,Lichtarge O,Hicks J,Weis MA,Eyre D,Lee BH

An additional function of the rough endoplasmic reticulum protein complex prolyl 3-hydroxylase 1?cartilage-associated protein?cyclophilin B: the CXXXC motif reveals disulfide isomerase activity in vitro.
The Journal of biological chemistry288(44)31437-46(2013 Nov)
Ishikawa Y,B?chinger HP

Generalized connective tissue disease in Crtap-/- mouse.
PloS one5(5)e10560(2010 May)
Baldridge D,Lennington J,Weis M,Homan EP,Jiang MM,Munivez E,Keene DR,Hogue WR,Pyott S,Byers PH,Krakow D,Cohn DH,Eyre DR,Lee B,Morello R

CRTAP is required for prolyl 3- hydroxylation and mutations cause recessive osteogenesis imperfecta.
Cell127(2)291-304(2006 Oct)
Morello R,Bertin TK,Chen Y,Hicks J,Tonachini L,Monticone M,Castagnola P,Rauch F,Glorieux FH,Vranka J,B?chinger HP,Pace JM,Schwarze U,Byers PH,Weis M,Fernandes RJ,Eyre DR,Yao Z,Boyce BF,Lee B

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Crtap cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報