Papolb cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Papolb
Entrez Gene ID	56522
Full Name	poly (A) polymerase beta (testis specific)
Synonyms	PAP,Paplob,Papola-ps,Papt,Plap-ps,Tpap
General protein information	Preferred Names poly (A) polymerase beta (testis specific) Names poly(A) polymerase beta PAP-beta polynucleotide adenylyltransferase beta testis-specific poly(A) polymerase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 G2

mRNA and Protein(s)

mRNA	Protein	Name
NM_019943.2	NP_064327.1	poly(A) polymerase beta

Pathways from BioSystems

Homologies

Homo sapiens (human)	PAPOLB	NP_064529.4
	PAPOLB	XP_002803238.1
Mus musculus (house mouse)	Papolb	NP_064327.1
Rattus norvegicus (Norway rat)	Papolb	NP_001012020.1
Pan troglodytes (chimpanzee)	PAPOLB	XP_003318331.1
Bos taurus (cattle)	PAPOLB	NP_001193351.1

Gene Ontology
Bibliography

Related articles in PubMed

PAPOLB/TPAP regulates spermiogenesis independently of chromatoid body-associated factors.
Kashiwabara SI, Tsuruta S, Yamaoka Y, Oyama K, Iwazaki C, Baba T
The Journal of reproduction and development(2017 Nov)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Germ cell-specific gene 1 targets testis-specific poly(A) polymerase to the endoplasmic reticulum through protein-protein interactions.
Choi HS, Lee SH, Kim H, Lee Y
FEBS letters582(8)1203-9(2008 Apr)

Infertility with defective spermiogenesis in mice lacking AF5q31, the target of chromosomal translocation in human infant leukemia.
Urano A, Endoh M, Wada T, Morikawa Y, Itoh M, Kataoka Y, Taki T, Akazawa H, Nakajima H, Komuro I, Yoshida N, Hayashi Y, Handa H, Kitamura T, Nosaka T
Molecular and cellular biology25(15)6834-45(2005 Aug)

Transgenic expression of testis-specific poly(A) polymerase TPAP in wild-type and TPAP-deficient mice.
Zhuang T, Kashiwabara S, Noguchi J, Baba T
The Journal of reproduction and development50(2)207-13(2004 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

expression of GSG1 stimulates TPAP targeting to the ER, suggesting that interactions between the two proteins lead to the redistribution of TPAP from the cytosol to the ER
Title: Germ cell-specific gene 1 targets testis-specific poly(A) polymerase to the endoplasmic reticulum through protein-protein interactions.

spermatogenesis requires the cytoplasmic elongation of the mRNA poly(A) tails catalyzed by TPAP
Title: Transgenic expression of testis-specific poly(A) polymerase TPAP in wild-type and TPAP-deficient mice.

propose that TPAP governs germ cell morphogenesis by modulating specific transcription factors at posttranscriptional and posttranslational levels
Title: Regulation of spermatogenesis by testis-specific, cytoplasmic poly(A) polymerase TPAP.

The following Papolb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Papolb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu58504	NM_019943.2 Latest version!	Mus musculus poly (A) polymerase beta (testis specific) (Papolb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$377.30 $539.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Papolb ( NM_019943.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_064327.1 Mus musculus poly (A) polymerase beta (testis specific) (Papolb), mRNA.

Price & Availability↑

CloneID	OMu58504
Clone ID Related Accession (Same CDS sequence)	NM_019943.2
Accession Version	NM_019943.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	poly(A) polymerase beta
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CAAA01187077.1. On Oct 21, 2005 this sequence version replaced NM_019943.1. ##Evidence-Data-START## Transcript is intronless :: AB030729.1 [ECO:0000345] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGCCAT TTGCGGTGAC CACCCAAGGA GCACAACAAC CGGCTCCCGC CCCGAAGCAG 
TTCGGCATAT CGTCCCCCAT CAGCCTGGCC GCCCCCAAGG ACACAGACCG CGAACTCACC 
CAGAAGCTGA TCGAGACCCT CCAGCCCTTC GGGGTGTTTG AAGAGGAAGA GGAACTGCAG 
CGCAGGATTT TAATTTTGCA GAAATTAAAT AATCTGGTGA AGGAATGGAT CCGAGAAATC 
AGTGAAAGCA GGAATCTTCC ACAAGCTGTA ATTGAGAATG TTGGGGGGAA AATTTTTACG 
TTCGGCTCTT ACCGATTAGG AGTACACACG AAAGGTGCAG ATATCGATGC TTTGTGCGTT 
GCACCAAGAC ACGTGGATCG AAATGACTTT TTCACCTCCT TCTATGACAA ATTGAAACTA 
CAAGAGGAAG TAAAAGATTT AAGAGCTGTT GAGGAGGCCT TTGTGCCGGT TATCAAACTG 
TGTTTTGATG GAATAGAGAT TGATATTTTG TTTGCAAGAT TAGCATTGCA GACTATTCCA 
GAAGATTTGG ACCTACGAGA TGATAGTCTG CTTAAAAATT TAGATATTAG GTGCATAAGA 
AGCCTTAACG GTTGCCGGGT AACTGATGAG ATTTTACATC TAGTACCAAA CATTGACAGC 
TTCAGGTTAA CACTGAGAGC CATCAAATTA TGGGCCAAAT GCCACAACAT CTATTCCAAT 
ATATTGGGTT TCCTTGGAGG TGTTTCCTGG GCTATGCTAG TAGCAAGAAC TTGCCAGCTT 
TACCCAAATG CAATAGCATC CACTCTTGTG CGAAAATTTT TCTTGGTGTT TTCTGAGTGG 
GAATGGCCAA ATCCAGTGTT GTTAAAAGAA CCAGAAGAAC GAAATCTTAA CTTGCCTGTA 
TGGGACCCAA GAGTGAATCC TAGTGACAGG TACCATCTTA TGCCTATAAT TACACCAGCA 
TACCCACAGC AGAACTCCAC GTACAATGTG TCTGTTTCGA CCAGGATGGT CATGATTGAG 
GAGTTTAAAC AAGGACTTGC TATCACACAT GAAATTCTGC TGAATAAGGC AGAATGGTCC 
AAACTTTTTG AAGCTCCAAG CTTTTTTCAA AAATACAAGC ATTATATTGT ACTTCTGGCA 
AGTGCGCCAA CAGAGAAACA ACATTTAGAG TGGGTGGGCT TGGTGGAATC AAAGATCCGA 
ATCCTGGTTG GGAGCCTGGA AAAGAATGAG TTCATTACTC TGGCTCATGT GAATCCTCAG 
TCGTTTCCAG CACCAAAAGA AACTGCTGAC AAGGAAGAAT TTCGTACTAT GTGGGTGATC 
GGGTTAGTGT TGAAGAAGCC AGAAAACTCT GAAATTCTCA GTATTGATCT CACCTATGAT 
ATTCAGTCTT TCACAGATAC AGTGTACAGA CAAGCAATAA ATAGTAAGAT GTTTGAGATG 
GATATGAAGA TTGCTGCAAT GCATCTAAGA AGGAAGGAAC TTCATCAGCT ACTTCCAAAT 
CATGTTCTTC AGAAAAAGGA AACCCATTTA ACAGAAAGTG TAAGGCTGAC AGCTGTGACT 
GACAGCAGCC TTCTCTTGTC TATAGACAGT GAAAACAGCA TGACTGCTCC TTCACCTACT 
GGCACTATGA AGACAGGTCC ACTGACTGGA AACCCTCAGG GCAGAAACAG TCCTGCTCTG 
GCTGTTATGG CAGCATCTGT GACCAACATA CAGTTTCCTG ATGTTTCCTT GCAGCATGTG 
AATCCCATAG AAAGCTCAGG AATTGCACTG AGTGAAAGCA TCCCTCAGAT TCCCTCACAA 
CCTACCATTT CACCACCACC TAAGCCTACA ATGACCAGAG TGGTTTCTTC GACACATCTG 
GTAAACCATC CATCTAGACC TTCAGGAAAT ACAGCAACGA ACATACCCAA TCCTATACTA 
GGAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064327.1
CDS	191..2119
Translation	MMPFAVTTQGAQQPAPAPKQFGISSPISLAAPKDTDRELTQKLIETLQPFGVFEEEEELQRRILILQKLNNLVKEWIREISESRNLPQAVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRNDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNIDSFRLTLRAIKLWAKCHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVRKFFLVFSEWEWPNPVLLKEPEERNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMIEEFKQGLAITHEILLNKAEWSKLFEAPSFFQKYKHYIVLLASAPTEKQHLEWVGLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKETADKEEFRTMWVIGLVLKKPENSEILSIDLTYDIQSFTDTVYRQAINSKMFEMDMKIAAMHLRRKELHQLLPNHVLQKKETHLTESVRLTAVTDSSLLLSIDSENSMTAPSPTGTMKTGPLTGNPQGRNSPALAVMAASVTNIQFPDVSLQHVNPIESSGIALSESIPQIPSQPTISPPPKPTMTRVVSSTHLVNHPSRPSGNTATNIPNPILGV

Target ORF information:

RefSeq Version	NM_019943.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus poly (A) polymerase beta (testis specific) (Papolb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019943.2

ORF Insert Sequence:

ATGATGCCAT TTGCGGTGAC CACCCAAGGA GCACAACAAC CGGCTCCCGC CCCGAAGCAG 
TTCGGCATAT CGTCCCCCAT CAGCCTGGCC GCCCCCAAGG ACACAGACCG CGAACTCACC 
CAGAAGCTGA TCGAGACCCT CCAGCCCTTC GGGGTGTTTG AAGAGGAAGA GGAACTGCAG 
CGCAGGATTT TAATTTTGCA GAAATTAAAT AATCTGGTGA AGGAATGGAT CCGAGAAATC 
AGTGAAAGCA GGAATCTTCC ACAAGCTGTA ATTGAGAATG TTGGGGGGAA AATTTTTACG 
TTCGGCTCTT ACCGATTAGG AGTACACACG AAAGGTGCAG ATATCGATGC TTTGTGCGTT 
GCACCAAGAC ACGTGGATCG AAATGACTTT TTCACCTCCT TCTATGACAA ATTGAAACTA 
CAAGAGGAAG TAAAAGATTT AAGAGCTGTT GAGGAGGCCT TTGTGCCGGT TATCAAACTG 
TGTTTTGATG GAATAGAGAT TGATATTTTG TTTGCAAGAT TAGCATTGCA GACTATTCCA 
GAAGATTTGG ACCTACGAGA TGATAGTCTG CTTAAAAATT TAGATATTAG GTGCATAAGA 
AGCCTTAACG GTTGCCGGGT AACTGATGAG ATTTTACATC TAGTACCAAA CATTGACAGC 
TTCAGGTTAA CACTGAGAGC CATCAAATTA TGGGCCAAAT GCCACAACAT CTATTCCAAT 
ATATTGGGTT TCCTTGGAGG TGTTTCCTGG GCTATGCTAG TAGCAAGAAC TTGCCAGCTT 
TACCCAAATG CAATAGCATC CACTCTTGTG CGAAAATTTT TCTTGGTGTT TTCTGAGTGG 
GAATGGCCAA ATCCAGTGTT GTTAAAAGAA CCAGAAGAAC GAAATCTTAA CTTGCCTGTA 
TGGGACCCAA GAGTGAATCC TAGTGACAGG TACCATCTTA TGCCTATAAT TACACCAGCA 
TACCCACAGC AGAACTCCAC GTACAATGTG TCTGTTTCGA CCAGGATGGT CATGATTGAG 
GAGTTTAAAC AAGGACTTGC TATCACACAT GAAATTCTGC TGAATAAGGC AGAATGGTCC 
AAACTTTTTG AAGCTCCAAG CTTTTTTCAA AAATACAAGC ATTATATTGT ACTTCTGGCA 
AGTGCGCCAA CAGAGAAACA ACATTTAGAG TGGGTGGGCT TGGTGGAATC AAAGATCCGA 
ATCCTGGTTG GGAGCCTGGA AAAGAATGAG TTCATTACTC TGGCTCATGT GAATCCTCAG 
TCGTTTCCAG CACCAAAAGA AACTGCTGAC AAGGAAGAAT TTCGTACTAT GTGGGTGATC 
GGGTTAGTGT TGAAGAAGCC AGAAAACTCT GAAATTCTCA GTATTGATCT CACCTATGAT 
ATTCAGTCTT TCACAGATAC AGTGTACAGA CAAGCAATAA ATAGTAAGAT GTTTGAGATG 
GATATGAAGA TTGCTGCAAT GCATCTAAGA AGGAAGGAAC TTCATCAGCT ACTTCCAAAT 
CATGTTCTTC AGAAAAAGGA AACCCATTTA ACAGAAAGTG TAAGGCTGAC AGCTGTGACT 
GACAGCAGCC TTCTCTTGTC TATAGACAGT GAAAACAGCA TGACTGCTCC TTCACCTACT 
GGCACTATGA AGACAGGTCC ACTGACTGGA AACCCTCAGG GCAGAAACAG TCCTGCTCTG 
GCTGTTATGG CAGCATCTGT GACCAACATA CAGTTTCCTG ATGTTTCCTT GCAGCATGTG 
AATCCCATAG AAAGCTCAGG AATTGCACTG AGTGAAAGCA TCCCTCAGAT TCCCTCACAA 
CCTACCATTT CACCACCACC TAAGCCTACA ATGACCAGAG TGGTTTCTTC GACACATCTG 
GTAAACCATC CATCTAGACC TTCAGGAAAT ACAGCAACGA ACATACCCAA TCCTATACTA 
GGAGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PAPOLB/TPAP regulates spermiogenesis independently of chromatoid body-associated factors.
The Journal of reproduction and development(2017 Nov)
Kashiwabara SI,Tsuruta S,Yamaoka Y,Oyama K,Iwazaki C,Baba T

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

Germ cell-specific gene 1 targets testis-specific poly(A) polymerase to the endoplasmic reticulum through protein-protein interactions.
FEBS letters582(8)1203-9(2008 Apr)
Choi HS,Lee SH,Kim H,Lee Y

Infertility with defective spermiogenesis in mice lacking AF5q31, the target of chromosomal translocation in human infant leukemia.
Molecular and cellular biology25(15)6834-45(2005 Aug)
Urano A,Endoh M,Wada T,Morikawa Y,Itoh M,Kataoka Y,Taki T,Akazawa H,Nakajima H,Komuro I,Yoshida N,Hayashi Y,Handa H,Kitamura T,Nosaka T

Transgenic expression of testis-specific poly(A) polymerase TPAP in wild-type and TPAP-deficient mice.
The Journal of reproduction and development50(2)207-13(2004 Apr)
Zhuang T,Kashiwabara S,Noguchi J,Baba T

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Papolb cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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