Dgke cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dgke
Entrez Gene ID	56077
Full Name	diacylglycerol kinase, epsilon
Synonyms	C87606,DAGK6,DGK
General protein information	Preferred Names diacylglycerol kinase, epsilon Names diacylglycerol kinase epsilon DAG kinase epsilon DGK-epsilon diglyceride kinase epsilon
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 C

mRNA and Protein(s)

mRNA	Protein	Name
NM_019505.3	NP_062378.1	diacylglycerol kinase epsilon
XM_011249151.2	XP_011247453.1	diacylglycerol kinase epsilon isoform X1
XM_011249151.3	XP_011247453.1	diacylglycerol kinase epsilon isoform X1
NM_019505.4	NP_062378.1	diacylglycerol kinase epsilon isoform 1
NM_001362845.1	NP_001349774.1	diacylglycerol kinase epsilon isoform 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DGKE	XP_548222.2
Arabidopsis thaliana (thale cress)	DGK1	NP_196409.1
Gallus gallus (chicken)	DGKE	XP_001234226.3
Mus musculus (house mouse)	Dgke	NP_062378.1
Danio rerio (zebrafish)	dgke	NP_001165699.1
Drosophila melanogaster (fruit fly)	Dgkepsilon	NP_725228.1
Caenorhabditis elegans (roundworm)	dgk-2	NP_001024679.1
Xenopus tropicalis (tropical clawed frog)	dgke	XP_002937855.1
Pan troglodytes (chimpanzee)	DGKE	XP_001171847.2
Homo sapiens (human)	DGKE	NP_003638.1
Bos taurus (cattle)	DGKE	NP_001179859.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006768	XP_308977.4
Oryza sativa (rice)	Os12g0576900	NP_001067111.1

Gene Ontology
Bibliography

Related articles in PubMed

Diacylglycerol kinase ? deficiency preserves glucose tolerance and modulates lipid metabolism in obese mice.
Manner?s-Holm L, Sch?nke M, Brozinick JT, Vetterli L, Bui HH, Sanders P, Nascimento EBM, Bj?rnholm M, Chibalin AV, Zierath JR
Journal of lipid research58(5)907-915(2017 May)

Diacylglycerol kinase epsilon suppresses expression of p53 and glycerol kinase in mouse embryo fibroblasts.
So V, Jalan D, Lemaire M, Topham MK, Hatch GM, Epand RM
Biochimica et biophysica acta1861(12 Pt A)1993-1999(2016 12)

Loss of diacylglycerol kinase epsilon in mice causes endothelial distress and impairs glomerular Cox-2 and PGE2 production.
Zhu J, Chaki M, Lu D, Ren C, Wang SS, Rauhauser A, Li B, Zimmerman S, Jun B, Du Y, Vadnagara K, Wang H, Elhadi S, Quigg RJ, Topham MK, Mohan C, Ozaltin F, Zhou XJ, Marciano DK, Bazan NG, Attanasio M
American journal of physiology. Renal physiology310(9)F895-908(2016 May)

Diacylglycerol Kinase-?: Properties and Biological Roles.
Epand RM, So V, Jennings W, Khadka B, Gupta RS, Lemaire M
Frontiers in cell and developmental biology4112(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DGKepsilon plays a role in glucose and energy homeostasis by modulating lipid metabolism in skeletal muscle
Title: Diacylglycerol kinase ? deficiency preserves glucose tolerance and modulates lipid metabolism in obese mice.

Our results unveil an unexpected role of Dgke in the induction of cyclooxygenase-2 and in the regulation of glomerular prostanoids synthesis under stress
Title: Loss of diacylglycerol kinase epsilon in mice causes endothelial distress and impairs glomerular Cox-2 and PGE2 production.

Inhibition of lipid signaling enzyme diacylglycerol kinase epsilon attenuates mutant huntingtin toxicity.
Title: Inhibition of lipid signaling enzyme diacylglycerol kinase epsilon attenuates mutant huntingtin toxicity.

DGKepsilon plays a significant role in determining the enrichment of GPInsP n with 20:4 and that there is a pathway for the selective translocation of arachidonoyl phosphatidic acid from the plasma membrane to the endoplasmic reticulum
Title: Dramatic differences in the roles in lipid metabolism of two isoforms of diacylglycerol kinase.

A regulatory role is suggested for DGK epsilon, COX-2, and catecholamine signaling during kindling epileptogenesis.
Title: Epileptogenesis in diacylglycerol kinase epsilon deficiency up-regulates COX-2 and tyrosine hydroxylase in hippocampus.

The following Dgke gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dgke cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20873	NM_019505.4 Latest version!	Mus musculus diacylglycerol kinase, epsilon (Dgke), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu84187	NM_001362845.1 Latest version!	Mus musculus diacylglycerol kinase, epsilon (Dgke), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu69610	XM_011249151.3 Latest version!	Mus musculus diacylglycerol kinase, epsilon (Dgke), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu69610	XM_011249151.2	Mus musculus diacylglycerol kinase, epsilon (Dgke), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu20873	NM_019505.3	Mus musculus diacylglycerol kinase, epsilon (Dgke), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dgke ( NM_019505.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062378.1 Mus musculus diacylglycerol kinase, epsilon (Dgke), mRNA.

Price & Availability↑

CloneID	OMu20873
Clone ID Related Accession (Same CDS sequence)	NM_019505.3 , NM_019505.4
Accession Version	NM_019505.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Mus musculus(house mouse)
Product	diacylglycerol kinase epsilon
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147841.1, BB635763.1, AK049335.1 and AL646096.8. On Mar 1, 2007 this sequence version replaced NM_019505.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.148780.1, AK049335.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139, SAMN01164141 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGGGG ACCAGCGGTC CGGCCCACCC GCCCAGAGCC TGCTCCCTGA TGGCCACTTG 
GTCCTATGGA CGCTGTGCTC CGTGCTGTTG CCGGTGTTCA TCACTTTATG GTGTAGCCTG 
CAGCGGTCGC GCCGGCAGCT GCACCGCAGG GATATCTTCC GCAAGAGCAA GCATTGCTGG 
CGTGACACCG ACCTGTTCAG CCACCCCACC TACTGCTGCG TGTGCGCGCA GCACATCCTG 
CAAGGAGCCT TCTGCGACTG CTGTGGGCTC CGCGTGGACG AGGGCTGCCT CAAGAAGGTC 
GACAAGCGCT TCCCGTGCAA GGAGATCATG CTCAAGAACG ACAAGGCCGC AGATGCCATG 
CCCCACCACT GGATCCGCGG CAACGTGCCC CTGTGCTCTT ACTGTGTATT CTGCAGGCAG 
CAGTGTGGCA GCCAGCCTAA ACTGTGCGAT TACAGGTGCA TTTGGTGCCA GAAGACAGTC 
CACGACGAGT GCATGCGAGG CAGCTTAAGG AGCGAAAAGT GTGACTTTGG AGAATTCAGA 
AACCTCATCA TCCCGCCAAG CTACTTAACT TCTATTAACC AGATGCGTAA AGACAAAAAC 
ACAAATTATG AAGGGCTTGC TTCTAAGTTT GGAAAGCAAT GGACCCCATT AATAATCCTA 
GCCAACTCTC GTAGTGGAAC TAACATGGGA GAAGGTCTGT TGGGAGAGTT TAAGATCCTG 
TTAAATCCAG TCCAGGTGTT TGATGTAACT AAAACTCCCC CGATCAAAGC TCTGCAGCTG 
TGCACTCTTC TTCCTTATTA CTCAGTTCGA GTCCTTGTTT GTGGAGGGGA CGGGACTGTG 
GGCTGGGTCC TCGATGCCAT TGATGAAATG AAGATTAAGG GACAAGAAAA ATACATTCCA 
GAGGTTGCGG TCTTACCTCT GGGAACAGGT AATGATCTGT CCAATACCCT GGGTTGGGGT 
ACAGGCTATG CTGGAGAAAT TCCGGTTGCA CAGGTCTTAA GAAATGTAAT GGAAGCAGAT 
GGAATCAAAC TAGACAGATG GAAAGTTCAG GTAACAAATA AAGGATACTA CAATTTAAGA 
AAACCCAAGG AGTTCACAAT GAACAACTAC TTCTCTGTTG GACCTGATGC CCTCATGGCT 
CTCAATTTTC ACGCTCATCG TGAGAAGGCA CCATCTCTGT TTTCCAGCCG AATCCTTAAC 
AAGGCTGTTT ATTTATTCTA TGGAACTAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAA TTGAGCTAGA ACTGGATGGT GAGCGAGTTG AGCTGCCTAA CTTGGAGGGC 
ATAATTGTTC TGAACATTGG ATACTGGGGC GGTGGCTGCA GACTGTGGGA AGGAATGGGA 
GATGAGACTT ACCCTCTAGC CAGACACGAT GATGGCTTAC TGGAAATCGT TGGCGTCTAT 
GGGTCTTTCC ACTGTGCTCA AATCCAGGTG AAACTGGCAA ATCCTTTTCG AATAGGACAA 
GCGCACACAG TGCGGCTGAC TTTGAAGTGC TCCATGATGC CGATGCAGGT GGATGGGGAG 
CCGTGGGCCC AAGGCCCCTG TACTGTCACC ATAACGCACA AGACACACGC ACTGATGCTG 
TACTTCTCTG GAGAGCAGTC AGATGACGAC ATCTCAAGCC CTTCGGATCA TGAGGACGTA 
AAGGAAGCCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062378.1
CDS	364..2058
Translation	MEGDQRSGPPAQSLLPDGHLVLWTLCSVLLPVFITLWCSLQRSRRQLHRRDIFRKSKHCWRDTDLFSHPTYCCVCAQHILQGAFCDCCGLRVDEGCLKKVDKRFPCKEIMLKNDKAADAMPHHWIRGNVPLCSYCVFCRQQCGSQPKLCDYRCIWCQKTVHDECMRGSLRSEKCDFGEFRNLIIPPSYLTSINQMRKDKNTNYEGLASKFGKQWTPLIILANSRSGTNMGEGLLGEFKILLNPVQVFDVTKTPPIKALQLCTLLPYYSVRVLVCGGDGTVGWVLDAIDEMKIKGQEKYIPEVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGIKLDRWKVQVTNKGYYNLRKPKEFTMNNYFSVGPDALMALNFHAHREKAPSLFSSRILNKAVYLFYGTKDCLVQECKDLNKKIELELDGERVELPNLEGIIVLNIGYWGGGCRLWEGMGDETYPLARHDDGLLEIVGVYGSFHCAQIQVKLANPFRIGQAHTVRLTLKCSMMPMQVDGEPWAQGPCTVTITHKTHALMLYFSGEQSDDDISSPSDHEDVKEAE

Target ORF information:

RefSeq Version	NM_019505.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus diacylglycerol kinase, epsilon (Dgke), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019505.3

ORF Insert Sequence:

ATGGAGGGGG ACCAGCGGTC CGGCCCACCC GCCCAGAGCC TGCTCCCTGA TGGCCACTTG 
GTCCTATGGA CGCTGTGCTC CGTGCTGTTG CCGGTGTTCA TCACTTTATG GTGTAGCCTG 
CAGCGGTCGC GCCGGCAGCT GCACCGCAGG GATATCTTCC GCAAGAGCAA GCATTGCTGG 
CGTGACACCG ACCTGTTCAG CCACCCCACC TACTGCTGCG TGTGCGCGCA GCACATCCTG 
CAAGGAGCCT TCTGCGACTG CTGTGGGCTC CGCGTGGACG AGGGCTGCCT CAAGAAGGTC 
GACAAGCGCT TCCCGTGCAA GGAGATCATG CTCAAGAACG ACAAGGCCGC AGATGCCATG 
CCCCACCACT GGATCCGCGG CAACGTGCCC CTGTGCTCTT ACTGTGTATT CTGCAGGCAG 
CAGTGTGGCA GCCAGCCTAA ACTGTGCGAT TACAGGTGCA TTTGGTGCCA GAAGACAGTC 
CACGACGAGT GCATGCGAGG CAGCTTAAGG AGCGAAAAGT GTGACTTTGG AGAATTCAGA 
AACCTCATCA TCCCGCCAAG CTACTTAACT TCTATTAACC AGATGCGTAA AGACAAAAAC 
ACAAATTATG AAGGGCTTGC TTCTAAGTTT GGAAAGCAAT GGACCCCATT AATAATCCTA 
GCCAACTCTC GTAGTGGAAC TAACATGGGA GAAGGTCTGT TGGGAGAGTT TAAGATCCTG 
TTAAATCCAG TCCAGGTGTT TGATGTAACT AAAACTCCCC CGATCAAAGC TCTGCAGCTG 
TGCACTCTTC TTCCTTATTA CTCAGTTCGA GTCCTTGTTT GTGGAGGGGA CGGGACTGTG 
GGCTGGGTCC TCGATGCCAT TGATGAAATG AAGATTAAGG GACAAGAAAA ATACATTCCA 
GAGGTTGCGG TCTTACCTCT GGGAACAGGT AATGATCTGT CCAATACCCT GGGTTGGGGT 
ACAGGCTATG CTGGAGAAAT TCCGGTTGCA CAGGTCTTAA GAAATGTAAT GGAAGCAGAT 
GGAATCAAAC TAGACAGATG GAAAGTTCAG GTAACAAATA AAGGATACTA CAATTTAAGA 
AAACCCAAGG AGTTCACAAT GAACAACTAC TTCTCTGTTG GACCTGATGC CCTCATGGCT 
CTCAATTTTC ACGCTCATCG TGAGAAGGCA CCATCTCTGT TTTCCAGCCG AATCCTTAAC 
AAGGCTGTTT ATTTATTCTA TGGAACTAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAA TTGAGCTAGA ACTGGATGGT GAGCGAGTTG AGCTGCCTAA CTTGGAGGGC 
ATAATTGTTC TGAACATTGG ATACTGGGGC GGTGGCTGCA GACTGTGGGA AGGAATGGGA 
GATGAGACTT ACCCTCTAGC CAGACACGAT GATGGCTTAC TGGAAATCGT TGGCGTCTAT 
GGGTCTTTCC ACTGTGCTCA AATCCAGGTG AAACTGGCAA ATCCTTTTCG AATAGGACAA 
GCGCACACAG TGCGGCTGAC TTTGAAGTGC TCCATGATGC CGATGCAGGT GGATGGGGAG 
CCGTGGGCCC AAGGCCCCTG TACTGTCACC ATAACGCACA AGACACACGC ACTGATGCTG 
TACTTCTCTG GAGAGCAGTC AGATGACGAC ATCTCAAGCC CTTCGGATCA TGAGGACGTA 
AAGGAAGCCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgke ( NM_019505.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062378.1 Mus musculus diacylglycerol kinase, epsilon (Dgke), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu20873
Clone ID Related Accession (Same CDS sequence)	NM_019505.3 , NM_019505.4
Accession Version	NM_019505.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	diacylglycerol kinase epsilon isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147841.1, BB635763.1, AK049335.1 and AL646096.8. On May 1, 2018 this sequence version replaced NM_019505.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.148780.1, AK049335.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139, SAMN01164141 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGGGG ACCAGCGGTC CGGCCCACCC GCCCAGAGCC TGCTCCCTGA TGGCCACTTG 
GTCCTATGGA CGCTGTGCTC CGTGCTGTTG CCGGTGTTCA TCACTTTATG GTGTAGCCTG 
CAGCGGTCGC GCCGGCAGCT GCACCGCAGG GATATCTTCC GCAAGAGCAA GCATTGCTGG 
CGTGACACCG ACCTGTTCAG CCACCCCACC TACTGCTGCG TGTGCGCGCA GCACATCCTG 
CAAGGAGCCT TCTGCGACTG CTGTGGGCTC CGCGTGGACG AGGGCTGCCT CAAGAAGGTC 
GACAAGCGCT TCCCGTGCAA GGAGATCATG CTCAAGAACG ACAAGGCCGC AGATGCCATG 
CCCCACCACT GGATCCGCGG CAACGTGCCC CTGTGCTCTT ACTGTGTATT CTGCAGGCAG 
CAGTGTGGCA GCCAGCCTAA ACTGTGCGAT TACAGGTGCA TTTGGTGCCA GAAGACAGTC 
CACGACGAGT GCATGCGAGG CAGCTTAAGG AGCGAAAAGT GTGACTTTGG AGAATTCAGA 
AACCTCATCA TCCCGCCAAG CTACTTAACT TCTATTAACC AGATGCGTAA AGACAAAAAC 
ACAAATTATG AAGGGCTTGC TTCTAAGTTT GGAAAGCAAT GGACCCCATT AATAATCCTA 
GCCAACTCTC GTAGTGGAAC TAACATGGGA GAAGGTCTGT TGGGAGAGTT TAAGATCCTG 
TTAAATCCAG TCCAGGTGTT TGATGTAACT AAAACTCCCC CGATCAAAGC TCTGCAGCTG 
TGCACTCTTC TTCCTTATTA CTCAGTTCGA GTCCTTGTTT GTGGAGGGGA CGGGACTGTG 
GGCTGGGTCC TCGATGCCAT TGATGAAATG AAGATTAAGG GACAAGAAAA ATACATTCCA 
GAGGTTGCGG TCTTACCTCT GGGAACAGGT AATGATCTGT CCAATACCCT GGGTTGGGGT 
ACAGGCTATG CTGGAGAAAT TCCGGTTGCA CAGGTCTTAA GAAATGTAAT GGAAGCAGAT 
GGAATCAAAC TAGACAGATG GAAAGTTCAG GTAACAAATA AAGGATACTA CAATTTAAGA 
AAACCCAAGG AGTTCACAAT GAACAACTAC TTCTCTGTTG GACCTGATGC CCTCATGGCT 
CTCAATTTTC ACGCTCATCG TGAGAAGGCA CCATCTCTGT TTTCCAGCCG AATCCTTAAC 
AAGGCTGTTT ATTTATTCTA TGGAACTAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAA TTGAGCTAGA ACTGGATGGT GAGCGAGTTG AGCTGCCTAA CTTGGAGGGC 
ATAATTGTTC TGAACATTGG ATACTGGGGC GGTGGCTGCA GACTGTGGGA AGGAATGGGA 
GATGAGACTT ACCCTCTAGC CAGACACGAT GATGGCTTAC TGGAAATCGT TGGCGTCTAT 
GGGTCTTTCC ACTGTGCTCA AATCCAGGTG AAACTGGCAA ATCCTTTTCG AATAGGACAA 
GCGCACACAG TGCGGCTGAC TTTGAAGTGC TCCATGATGC CGATGCAGGT GGATGGGGAG 
CCGTGGGCCC AAGGCCCCTG TACTGTCACC ATAACGCACA AGACACACGC ACTGATGCTG 
TACTTCTCTG GAGAGCAGTC AGATGACGAC ATCTCAAGCC CTTCGGATCA TGAGGACGTA 
AAGGAAGCCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062378.1
CDS	364..2058
Translation	MEGDQRSGPPAQSLLPDGHLVLWTLCSVLLPVFITLWCSLQRSRRQLHRRDIFRKSKHCWRDTDLFSHPTYCCVCAQHILQGAFCDCCGLRVDEGCLKKVDKRFPCKEIMLKNDKAADAMPHHWIRGNVPLCSYCVFCRQQCGSQPKLCDYRCIWCQKTVHDECMRGSLRSEKCDFGEFRNLIIPPSYLTSINQMRKDKNTNYEGLASKFGKQWTPLIILANSRSGTNMGEGLLGEFKILLNPVQVFDVTKTPPIKALQLCTLLPYYSVRVLVCGGDGTVGWVLDAIDEMKIKGQEKYIPEVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGIKLDRWKVQVTNKGYYNLRKPKEFTMNNYFSVGPDALMALNFHAHREKAPSLFSSRILNKAVYLFYGTKDCLVQECKDLNKKIELELDGERVELPNLEGIIVLNIGYWGGGCRLWEGMGDETYPLARHDDGLLEIVGVYGSFHCAQIQVKLANPFRIGQAHTVRLTLKCSMMPMQVDGEPWAQGPCTVTITHKTHALMLYFSGEQSDDDISSPSDHEDVKEAE

Target ORF information:

RefSeq Version	NM_019505.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus diacylglycerol kinase, epsilon (Dgke), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019505.4

ORF Insert Sequence:

ATGGAGGGGG ACCAGCGGTC CGGCCCACCC GCCCAGAGCC TGCTCCCTGA TGGCCACTTG 
GTCCTATGGA CGCTGTGCTC CGTGCTGTTG CCGGTGTTCA TCACTTTATG GTGTAGCCTG 
CAGCGGTCGC GCCGGCAGCT GCACCGCAGG GATATCTTCC GCAAGAGCAA GCATTGCTGG 
CGTGACACCG ACCTGTTCAG CCACCCCACC TACTGCTGCG TGTGCGCGCA GCACATCCTG 
CAAGGAGCCT TCTGCGACTG CTGTGGGCTC CGCGTGGACG AGGGCTGCCT CAAGAAGGTC 
GACAAGCGCT TCCCGTGCAA GGAGATCATG CTCAAGAACG ACAAGGCCGC AGATGCCATG 
CCCCACCACT GGATCCGCGG CAACGTGCCC CTGTGCTCTT ACTGTGTATT CTGCAGGCAG 
CAGTGTGGCA GCCAGCCTAA ACTGTGCGAT TACAGGTGCA TTTGGTGCCA GAAGACAGTC 
CACGACGAGT GCATGCGAGG CAGCTTAAGG AGCGAAAAGT GTGACTTTGG AGAATTCAGA 
AACCTCATCA TCCCGCCAAG CTACTTAACT TCTATTAACC AGATGCGTAA AGACAAAAAC 
ACAAATTATG AAGGGCTTGC TTCTAAGTTT GGAAAGCAAT GGACCCCATT AATAATCCTA 
GCCAACTCTC GTAGTGGAAC TAACATGGGA GAAGGTCTGT TGGGAGAGTT TAAGATCCTG 
TTAAATCCAG TCCAGGTGTT TGATGTAACT AAAACTCCCC CGATCAAAGC TCTGCAGCTG 
TGCACTCTTC TTCCTTATTA CTCAGTTCGA GTCCTTGTTT GTGGAGGGGA CGGGACTGTG 
GGCTGGGTCC TCGATGCCAT TGATGAAATG AAGATTAAGG GACAAGAAAA ATACATTCCA 
GAGGTTGCGG TCTTACCTCT GGGAACAGGT AATGATCTGT CCAATACCCT GGGTTGGGGT 
ACAGGCTATG CTGGAGAAAT TCCGGTTGCA CAGGTCTTAA GAAATGTAAT GGAAGCAGAT 
GGAATCAAAC TAGACAGATG GAAAGTTCAG GTAACAAATA AAGGATACTA CAATTTAAGA 
AAACCCAAGG AGTTCACAAT GAACAACTAC TTCTCTGTTG GACCTGATGC CCTCATGGCT 
CTCAATTTTC ACGCTCATCG TGAGAAGGCA CCATCTCTGT TTTCCAGCCG AATCCTTAAC 
AAGGCTGTTT ATTTATTCTA TGGAACTAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAA TTGAGCTAGA ACTGGATGGT GAGCGAGTTG AGCTGCCTAA CTTGGAGGGC 
ATAATTGTTC TGAACATTGG ATACTGGGGC GGTGGCTGCA GACTGTGGGA AGGAATGGGA 
GATGAGACTT ACCCTCTAGC CAGACACGAT GATGGCTTAC TGGAAATCGT TGGCGTCTAT 
GGGTCTTTCC ACTGTGCTCA AATCCAGGTG AAACTGGCAA ATCCTTTTCG AATAGGACAA 
GCGCACACAG TGCGGCTGAC TTTGAAGTGC TCCATGATGC CGATGCAGGT GGATGGGGAG 
CCGTGGGCCC AAGGCCCCTG TACTGTCACC ATAACGCACA AGACACACGC ACTGATGCTG 
TACTTCTCTG GAGAGCAGTC AGATGACGAC ATCTCAAGCC CTTCGGATCA TGAGGACGTA 
AAGGAAGCCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgke ( XM_011249151.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247453.1 Mus musculus diacylglycerol kinase, epsilon (Dgke), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu69610
Clone ID Related Accession (Same CDS sequence)	XM_011249151.2 , XM_011249151.3
Accession Version	XM_011249151.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	diacylglycerol kinase epsilon isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249151.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGG ACCAGCGGTC CGGCCCACCC GCCCAGAGCC TGCTCCCTGA TGGCCACTTG 
GTCCTATGGA CGCTGTGCTC CGTGCTGTTG CCGGTGTTCA TCACTTTATG GTGTAGCCTG 
CAGCGGTCGC GCCGGCAGCT GCACCGCAGG GATATCTTCC GCAAGAGCAA GCATTGCTGG 
CGTGACACCG ACCTGTTCAG CCACCCCACC TACTGCTGCG TGTGCGCGCA GCACATCCTG 
CAAGGAGCCT TCTGCGACTG CTGTGGGCTC CGCGTGGACG AGGGCTGCCT CAAGAAGGTC 
GACAAGCGCT TCCCGTGCAA GGAGATCATG CTCAAGAACG ACAAGGCCGC AGATGCCATG 
CCCCACCACT GGATCCGCGG CAACGTGCCC CTGTGCTCTT ACTGTGTATT CTGCAGGCAG 
CAGTGTGGCA GCCAGCCTAA ACTGTGCGAT TACAGGTGCA TTTGGTGCCA GAAGACAGTC 
CACGACGAGT GCATGCGAGG CAGCTTAAGG AGCGAAAAGT GTGACTTTGG AGAATTCAGA 
AACCTCATCA TCCCGCCAAG CTACTTAACT TCTATTAACC AGATGCGTAA AGACAAAAAC 
ACAAATTATG AAGGGCTTGC TTCTAAGTTT GGAAAGCAAT GGACCCCATT AATAATCCTA 
GCCAACTCTC GTAGTGGAAC TAACATGGGA GAAGGTCTGT TGGGAGAGTT TAAGATCCTG 
TTAAATCCAG TCCAGGTGTT TGATGTAACT AAAACTCCCC CGATCAAAGC TCTGCAGCTG 
TGCACTCTTC TTCCTTATTA CTCAGTTCGA GTCCTTGTTT GTGGAGGGGA CGGGACTGTG 
GGCTGGGTCC TCGATGCCAT TGATGAAATG AAGATTAAGG GACAAGAAAA ATACATTCCA 
GAGGTTGCGG TCTTACCTCT GGGAACAGGT AATGATCTGT CCAATACCCT GGGTTGGGGT 
ACAGGCTATG CTGGAGAAAT TCCGGTTGCA CAGGTCTTAA GAAATGTAAT GGAAGCAGAT 
GGAATCAAAC TAGACAGATG GAAAGTTCAG GTAACAAATA AAGGATACTA CAATTTAAGA 
AAACCCAAGG AGTTCACAAT GAACAACTAC TTCTCTGTTG GACCTGATGC CCTCATGGCT 
CTCAATTTTC ACGCTCATCG TGAGAAGGCA CCATCTCTGT TTTCCAGCCG AATCCTTAAC 
AAGGCTGTTT ATTTATTCTA TGGAACTAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAA TTGAGCTAGA ACTGGATGGT GAGCGAGTTG AGCTGCCTAA CTTGGAGGGC 
ATAATTGTTC TGAACATTGG ATACTGGGGC GGTGGCTGCA GACTGTGGGA AGGAATGGGA 
GATGAGACTT ACCCTCTAGC CAGACACGAT GATGGCTTAC TGGAAATCGT TGGCGTCTAT 
GGGTCTTTCC ACTGTGCTCA AATCCAGGTG AAACTGGCAA ATCCTTTTCG AATAGGACAA 
GCGCACACAG TGCGGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247453.1
CDS	207..1727
Translation	MEGDQRSGPPAQSLLPDGHLVLWTLCSVLLPVFITLWCSLQRSRRQLHRRDIFRKSKHCWRDTDLFSHPTYCCVCAQHILQGAFCDCCGLRVDEGCLKKVDKRFPCKEIMLKNDKAADAMPHHWIRGNVPLCSYCVFCRQQCGSQPKLCDYRCIWCQKTVHDECMRGSLRSEKCDFGEFRNLIIPPSYLTSINQMRKDKNTNYEGLASKFGKQWTPLIILANSRSGTNMGEGLLGEFKILLNPVQVFDVTKTPPIKALQLCTLLPYYSVRVLVCGGDGTVGWVLDAIDEMKIKGQEKYIPEVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGIKLDRWKVQVTNKGYYNLRKPKEFTMNNYFSVGPDALMALNFHAHREKAPSLFSSRILNKAVYLFYGTKDCLVQECKDLNKKIELELDGERVELPNLEGIIVLNIGYWGGGCRLWEGMGDETYPLARHDDGLLEIVGVYGSFHCAQIQVKLANPFRIGQAHTVRS

Target ORF information:

RefSeq Version	XM_011249151.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus diacylglycerol kinase, epsilon (Dgke), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249151.2

ORF Insert Sequence:

ATGGAGGGGG ACCAGCGGTC CGGCCCACCC GCCCAGAGCC TGCTCCCTGA TGGCCACTTG 
GTCCTATGGA CGCTGTGCTC CGTGCTGTTG CCGGTGTTCA TCACTTTATG GTGTAGCCTG 
CAGCGGTCGC GCCGGCAGCT GCACCGCAGG GATATCTTCC GCAAGAGCAA GCATTGCTGG 
CGTGACACCG ACCTGTTCAG CCACCCCACC TACTGCTGCG TGTGCGCGCA GCACATCCTG 
CAAGGAGCCT TCTGCGACTG CTGTGGGCTC CGCGTGGACG AGGGCTGCCT CAAGAAGGTC 
GACAAGCGCT TCCCGTGCAA GGAGATCATG CTCAAGAACG ACAAGGCCGC AGATGCCATG 
CCCCACCACT GGATCCGCGG CAACGTGCCC CTGTGCTCTT ACTGTGTATT CTGCAGGCAG 
CAGTGTGGCA GCCAGCCTAA ACTGTGCGAT TACAGGTGCA TTTGGTGCCA GAAGACAGTC 
CACGACGAGT GCATGCGAGG CAGCTTAAGG AGCGAAAAGT GTGACTTTGG AGAATTCAGA 
AACCTCATCA TCCCGCCAAG CTACTTAACT TCTATTAACC AGATGCGTAA AGACAAAAAC 
ACAAATTATG AAGGGCTTGC TTCTAAGTTT GGAAAGCAAT GGACCCCATT AATAATCCTA 
GCCAACTCTC GTAGTGGAAC TAACATGGGA GAAGGTCTGT TGGGAGAGTT TAAGATCCTG 
TTAAATCCAG TCCAGGTGTT TGATGTAACT AAAACTCCCC CGATCAAAGC TCTGCAGCTG 
TGCACTCTTC TTCCTTATTA CTCAGTTCGA GTCCTTGTTT GTGGAGGGGA CGGGACTGTG 
GGCTGGGTCC TCGATGCCAT TGATGAAATG AAGATTAAGG GACAAGAAAA ATACATTCCA 
GAGGTTGCGG TCTTACCTCT GGGAACAGGT AATGATCTGT CCAATACCCT GGGTTGGGGT 
ACAGGCTATG CTGGAGAAAT TCCGGTTGCA CAGGTCTTAA GAAATGTAAT GGAAGCAGAT 
GGAATCAAAC TAGACAGATG GAAAGTTCAG GTAACAAATA AAGGATACTA CAATTTAAGA 
AAACCCAAGG AGTTCACAAT GAACAACTAC TTCTCTGTTG GACCTGATGC CCTCATGGCT 
CTCAATTTTC ACGCTCATCG TGAGAAGGCA CCATCTCTGT TTTCCAGCCG AATCCTTAAC 
AAGGCTGTTT ATTTATTCTA TGGAACTAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAA TTGAGCTAGA ACTGGATGGT GAGCGAGTTG AGCTGCCTAA CTTGGAGGGC 
ATAATTGTTC TGAACATTGG ATACTGGGGC GGTGGCTGCA GACTGTGGGA AGGAATGGGA 
GATGAGACTT ACCCTCTAGC CAGACACGAT GATGGCTTAC TGGAAATCGT TGGCGTCTAT 
GGGTCTTTCC ACTGTGCTCA AATCCAGGTG AAACTGGCAA ATCCTTTTCG AATAGGACAA 
GCGCACACAG TGCGGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgke ( NM_001362845.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001349774.1 Mus musculus diacylglycerol kinase, epsilon (Dgke), transcript variant 2, mRNA.

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CloneID	OMu84187
Clone ID Related Accession (Same CDS sequence)	NM_001362845.1
Accession Version	NM_001362845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	diacylglycerol kinase epsilon isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL646096.8. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.11324.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139, SAMN01164141 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGGGG ACCAGCGGTC CGGCCCACCC GCCCAGAGCC TGCTCCCTGA TGGCCACTTG 
GTCCTATGGA CGCTGTGCTC CGTGCTGTTG CCGGTGTTCA TCACTTTATG GTGTAGCCTG 
CAGCGGTCGC GCCGGCAGCT GCACCGCAGG GATATCTTCC GCAAGAGCAA GCATTGCTGG 
CGTGACACCG ACCTGTTCAG CCACCCCACC TACTGCTGCG TGTGCGCGCA GCACATCCTG 
CAAGGAGCCT TCTGCGACTG CTGTGGGCTC CGCGTGGACG AGGGCTGCCT CAAGAAGGTC 
GACAAGCGCT TCCCGTGCAA GGAGATCATG CTCAAGAACG ACAAGGCCGC AGATGCCATG 
CCCCACCACT GGATCCGCGG CAACGTGCCC CTGTGCTCTT ACTGTGTATT CTGCAGGCAG 
CAGTGTGGCA GCCAGCCTAA ACTGTGCGAT TACAGGTGCA TTTGGTGCCA GAAGACAGTC 
CACGACGAGT GCATGCGAGG CAGCTTAAGG AGCGAAAAGT GTGACTTTGG AGAATTCAGA 
AACCTCATCA TCCCGCCAAG CTACTTAACT TCTATTAACC AGATGCGTAA AGACAAAAAC 
ACAAATTATG AAGGGCTTGC TTCTAAGTTT GGAAAGCAAT GGACCCCATT AATAATCCTA 
GCCAACTCTC GTAGTGGAAC TAACATGGGA GAAGGTCTGT TGGGAGAGTT TAAGATCCTG 
TTAAATCCAG TCCAGGTGTT TGATGTAACT AAAACTCCCC CGATCAAAGC TCTGCAGCTG 
TGCACTCTTC TTCCTTATTA CTCAGTTCGA GTCCTTGTTT GTGGAGGGGA CGGGACTGTG 
GGCTGGGTCC TCGATGCCAT TGATGAAATG AAGATTAAGG GACAAGAAAA ATACATTCCA 
GAGGTTGCGG TCTTACCTCT GGGAACAGGT AATGATCTGT CCAATACCCT GGGTTGGGGT 
ACAGGCTATG CTGGAGAAAT TCCGGTTGCA CAGGTCTTAA GAAATGTAAT GGAAGCAGAT 
GGAATCAAAC TAGACAGATG GAAAGTTCAG GTAACAAATA AAGGATACTA CAATTTAAGA 
AAACCCAAGG AGTTCACAAT GAACAACTAC TTCTCTGTTG GACCTGATGC CCTCATGGCT 
CTCAATTTTC ACGCTCATCG TGAGAAGGCA CCATCTCTGT TTTCCAGCCG AATCCTTAAC 
AAGGCTGTTT ATTTATTCTA TGGAACTAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAA TTGAGCTAGA ACTGGATGGT GAGCGAGTTG AGCTGCCTAA CTTGGAGGGC 
ATAATTGTTC TGAACATTGG ATACTGGGGC GGTGGCTGCA GACTGTGGGA AGGAATGGGA 
GATGAGACTT ACCCTCTAGC CAGACACGAT GATGGCTTAC TGGAAATCGT TGGCGTCTAT 
GGGTCTTTCC ACTGTGCTCA AATCCAGCTG ACTTTGAAGT GCTCCATGAT GCCGATGCAG 
GTGGATGGGG AGCCGTGGGC CCAAGGCCCC TGTACTGTCA CCATAACGCA CAAGACACAC 
GCACTGATGC TGTACTTCTC TGGAGAGCAG TCAGATGACG ACATCTCAAG CCCTTCGGAT 
CATGAGGACG TAAAGGAAGC CGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349774.1
CDS	364..2010
Translation	MEGDQRSGPPAQSLLPDGHLVLWTLCSVLLPVFITLWCSLQRSRRQLHRRDIFRKSKHCWRDTDLFSHPTYCCVCAQHILQGAFCDCCGLRVDEGCLKKVDKRFPCKEIMLKNDKAADAMPHHWIRGNVPLCSYCVFCRQQCGSQPKLCDYRCIWCQKTVHDECMRGSLRSEKCDFGEFRNLIIPPSYLTSINQMRKDKNTNYEGLASKFGKQWTPLIILANSRSGTNMGEGLLGEFKILLNPVQVFDVTKTPPIKALQLCTLLPYYSVRVLVCGGDGTVGWVLDAIDEMKIKGQEKYIPEVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGIKLDRWKVQVTNKGYYNLRKPKEFTMNNYFSVGPDALMALNFHAHREKAPSLFSSRILNKAVYLFYGTKDCLVQECKDLNKKIELELDGERVELPNLEGIIVLNIGYWGGGCRLWEGMGDETYPLARHDDGLLEIVGVYGSFHCAQIQLTLKCSMMPMQVDGEPWAQGPCTVTITHKTHALMLYFSGEQSDDDISSPSDHEDVKEAE

Target ORF information:

RefSeq Version	NM_001362845.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus diacylglycerol kinase, epsilon (Dgke), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362845.1

ORF Insert Sequence:

ATGGAGGGGG ACCAGCGGTC CGGCCCACCC GCCCAGAGCC TGCTCCCTGA TGGCCACTTG 
GTCCTATGGA CGCTGTGCTC CGTGCTGTTG CCGGTGTTCA TCACTTTATG GTGTAGCCTG 
CAGCGGTCGC GCCGGCAGCT GCACCGCAGG GATATCTTCC GCAAGAGCAA GCATTGCTGG 
CGTGACACCG ACCTGTTCAG CCACCCCACC TACTGCTGCG TGTGCGCGCA GCACATCCTG 
CAAGGAGCCT TCTGCGACTG CTGTGGGCTC CGCGTGGACG AGGGCTGCCT CAAGAAGGTC 
GACAAGCGCT TCCCGTGCAA GGAGATCATG CTCAAGAACG ACAAGGCCGC AGATGCCATG 
CCCCACCACT GGATCCGCGG CAACGTGCCC CTGTGCTCTT ACTGTGTATT CTGCAGGCAG 
CAGTGTGGCA GCCAGCCTAA ACTGTGCGAT TACAGGTGCA TTTGGTGCCA GAAGACAGTC 
CACGACGAGT GCATGCGAGG CAGCTTAAGG AGCGAAAAGT GTGACTTTGG AGAATTCAGA 
AACCTCATCA TCCCGCCAAG CTACTTAACT TCTATTAACC AGATGCGTAA AGACAAAAAC 
ACAAATTATG AAGGGCTTGC TTCTAAGTTT GGAAAGCAAT GGACCCCATT AATAATCCTA 
GCCAACTCTC GTAGTGGAAC TAACATGGGA GAAGGTCTGT TGGGAGAGTT TAAGATCCTG 
TTAAATCCAG TCCAGGTGTT TGATGTAACT AAAACTCCCC CGATCAAAGC TCTGCAGCTG 
TGCACTCTTC TTCCTTATTA CTCAGTTCGA GTCCTTGTTT GTGGAGGGGA CGGGACTGTG 
GGCTGGGTCC TCGATGCCAT TGATGAAATG AAGATTAAGG GACAAGAAAA ATACATTCCA 
GAGGTTGCGG TCTTACCTCT GGGAACAGGT AATGATCTGT CCAATACCCT GGGTTGGGGT 
ACAGGCTATG CTGGAGAAAT TCCGGTTGCA CAGGTCTTAA GAAATGTAAT GGAAGCAGAT 
GGAATCAAAC TAGACAGATG GAAAGTTCAG GTAACAAATA AAGGATACTA CAATTTAAGA 
AAACCCAAGG AGTTCACAAT GAACAACTAC TTCTCTGTTG GACCTGATGC CCTCATGGCT 
CTCAATTTTC ACGCTCATCG TGAGAAGGCA CCATCTCTGT TTTCCAGCCG AATCCTTAAC 
AAGGCTGTTT ATTTATTCTA TGGAACTAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAA TTGAGCTAGA ACTGGATGGT GAGCGAGTTG AGCTGCCTAA CTTGGAGGGC 
ATAATTGTTC TGAACATTGG ATACTGGGGC GGTGGCTGCA GACTGTGGGA AGGAATGGGA 
GATGAGACTT ACCCTCTAGC CAGACACGAT GATGGCTTAC TGGAAATCGT TGGCGTCTAT 
GGGTCTTTCC ACTGTGCTCA AATCCAGCTG ACTTTGAAGT GCTCCATGAT GCCGATGCAG 
GTGGATGGGG AGCCGTGGGC CCAAGGCCCC TGTACTGTCA CCATAACGCA CAAGACACAC 
GCACTGATGC TGTACTTCTC TGGAGAGCAG TCAGATGACG ACATCTCAAG CCCTTCGGAT 
CATGAGGACG TAAAGGAAGC CGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgke ( XM_011249151.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247453.1 Mus musculus diacylglycerol kinase, epsilon (Dgke), transcript variant X1, mRNA.

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CloneID	OMu69610
Clone ID Related Accession (Same CDS sequence)	XM_011249151.2 , XM_011249151.3
Accession Version	XM_011249151.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	diacylglycerol kinase epsilon isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011249151.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGG ACCAGCGGTC CGGCCCACCC GCCCAGAGCC TGCTCCCTGA TGGCCACTTG 
GTCCTATGGA CGCTGTGCTC CGTGCTGTTG CCGGTGTTCA TCACTTTATG GTGTAGCCTG 
CAGCGGTCGC GCCGGCAGCT GCACCGCAGG GATATCTTCC GCAAGAGCAA GCATTGCTGG 
CGTGACACCG ACCTGTTCAG CCACCCCACC TACTGCTGCG TGTGCGCGCA GCACATCCTG 
CAAGGAGCCT TCTGCGACTG CTGTGGGCTC CGCGTGGACG AGGGCTGCCT CAAGAAGGTC 
GACAAGCGCT TCCCGTGCAA GGAGATCATG CTCAAGAACG ACAAGGCCGC AGATGCCATG 
CCCCACCACT GGATCCGCGG CAACGTGCCC CTGTGCTCTT ACTGTGTATT CTGCAGGCAG 
CAGTGTGGCA GCCAGCCTAA ACTGTGCGAT TACAGGTGCA TTTGGTGCCA GAAGACAGTC 
CACGACGAGT GCATGCGAGG CAGCTTAAGG AGCGAAAAGT GTGACTTTGG AGAATTCAGA 
AACCTCATCA TCCCGCCAAG CTACTTAACT TCTATTAACC AGATGCGTAA AGACAAAAAC 
ACAAATTATG AAGGGCTTGC TTCTAAGTTT GGAAAGCAAT GGACCCCATT AATAATCCTA 
GCCAACTCTC GTAGTGGAAC TAACATGGGA GAAGGTCTGT TGGGAGAGTT TAAGATCCTG 
TTAAATCCAG TCCAGGTGTT TGATGTAACT AAAACTCCCC CGATCAAAGC TCTGCAGCTG 
TGCACTCTTC TTCCTTATTA CTCAGTTCGA GTCCTTGTTT GTGGAGGGGA CGGGACTGTG 
GGCTGGGTCC TCGATGCCAT TGATGAAATG AAGATTAAGG GACAAGAAAA ATACATTCCA 
GAGGTTGCGG TCTTACCTCT GGGAACAGGT AATGATCTGT CCAATACCCT GGGTTGGGGT 
ACAGGCTATG CTGGAGAAAT TCCGGTTGCA CAGGTCTTAA GAAATGTAAT GGAAGCAGAT 
GGAATCAAAC TAGACAGATG GAAAGTTCAG GTAACAAATA AAGGATACTA CAATTTAAGA 
AAACCCAAGG AGTTCACAAT GAACAACTAC TTCTCTGTTG GACCTGATGC CCTCATGGCT 
CTCAATTTTC ACGCTCATCG TGAGAAGGCA CCATCTCTGT TTTCCAGCCG AATCCTTAAC 
AAGGCTGTTT ATTTATTCTA TGGAACTAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAA TTGAGCTAGA ACTGGATGGT GAGCGAGTTG AGCTGCCTAA CTTGGAGGGC 
ATAATTGTTC TGAACATTGG ATACTGGGGC GGTGGCTGCA GACTGTGGGA AGGAATGGGA 
GATGAGACTT ACCCTCTAGC CAGACACGAT GATGGCTTAC TGGAAATCGT TGGCGTCTAT 
GGGTCTTTCC ACTGTGCTCA AATCCAGGTG AAACTGGCAA ATCCTTTTCG AATAGGACAA 
GCGCACACAG TGCGGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247453.1
CDS	411..1931
Translation	MEGDQRSGPPAQSLLPDGHLVLWTLCSVLLPVFITLWCSLQRSRRQLHRRDIFRKSKHCWRDTDLFSHPTYCCVCAQHILQGAFCDCCGLRVDEGCLKKVDKRFPCKEIMLKNDKAADAMPHHWIRGNVPLCSYCVFCRQQCGSQPKLCDYRCIWCQKTVHDECMRGSLRSEKCDFGEFRNLIIPPSYLTSINQMRKDKNTNYEGLASKFGKQWTPLIILANSRSGTNMGEGLLGEFKILLNPVQVFDVTKTPPIKALQLCTLLPYYSVRVLVCGGDGTVGWVLDAIDEMKIKGQEKYIPEVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGIKLDRWKVQVTNKGYYNLRKPKEFTMNNYFSVGPDALMALNFHAHREKAPSLFSSRILNKAVYLFYGTKDCLVQECKDLNKKIELELDGERVELPNLEGIIVLNIGYWGGGCRLWEGMGDETYPLARHDDGLLEIVGVYGSFHCAQIQVKLANPFRIGQAHTVRS

Target ORF information:

RefSeq Version	XM_011249151.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus diacylglycerol kinase, epsilon (Dgke), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249151.3

ORF Insert Sequence:

ATGGAGGGGG ACCAGCGGTC CGGCCCACCC GCCCAGAGCC TGCTCCCTGA TGGCCACTTG 
GTCCTATGGA CGCTGTGCTC CGTGCTGTTG CCGGTGTTCA TCACTTTATG GTGTAGCCTG 
CAGCGGTCGC GCCGGCAGCT GCACCGCAGG GATATCTTCC GCAAGAGCAA GCATTGCTGG 
CGTGACACCG ACCTGTTCAG CCACCCCACC TACTGCTGCG TGTGCGCGCA GCACATCCTG 
CAAGGAGCCT TCTGCGACTG CTGTGGGCTC CGCGTGGACG AGGGCTGCCT CAAGAAGGTC 
GACAAGCGCT TCCCGTGCAA GGAGATCATG CTCAAGAACG ACAAGGCCGC AGATGCCATG 
CCCCACCACT GGATCCGCGG CAACGTGCCC CTGTGCTCTT ACTGTGTATT CTGCAGGCAG 
CAGTGTGGCA GCCAGCCTAA ACTGTGCGAT TACAGGTGCA TTTGGTGCCA GAAGACAGTC 
CACGACGAGT GCATGCGAGG CAGCTTAAGG AGCGAAAAGT GTGACTTTGG AGAATTCAGA 
AACCTCATCA TCCCGCCAAG CTACTTAACT TCTATTAACC AGATGCGTAA AGACAAAAAC 
ACAAATTATG AAGGGCTTGC TTCTAAGTTT GGAAAGCAAT GGACCCCATT AATAATCCTA 
GCCAACTCTC GTAGTGGAAC TAACATGGGA GAAGGTCTGT TGGGAGAGTT TAAGATCCTG 
TTAAATCCAG TCCAGGTGTT TGATGTAACT AAAACTCCCC CGATCAAAGC TCTGCAGCTG 
TGCACTCTTC TTCCTTATTA CTCAGTTCGA GTCCTTGTTT GTGGAGGGGA CGGGACTGTG 
GGCTGGGTCC TCGATGCCAT TGATGAAATG AAGATTAAGG GACAAGAAAA ATACATTCCA 
GAGGTTGCGG TCTTACCTCT GGGAACAGGT AATGATCTGT CCAATACCCT GGGTTGGGGT 
ACAGGCTATG CTGGAGAAAT TCCGGTTGCA CAGGTCTTAA GAAATGTAAT GGAAGCAGAT 
GGAATCAAAC TAGACAGATG GAAAGTTCAG GTAACAAATA AAGGATACTA CAATTTAAGA 
AAACCCAAGG AGTTCACAAT GAACAACTAC TTCTCTGTTG GACCTGATGC CCTCATGGCT 
CTCAATTTTC ACGCTCATCG TGAGAAGGCA CCATCTCTGT TTTCCAGCCG AATCCTTAAC 
AAGGCTGTTT ATTTATTCTA TGGAACTAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAA TTGAGCTAGA ACTGGATGGT GAGCGAGTTG AGCTGCCTAA CTTGGAGGGC 
ATAATTGTTC TGAACATTGG ATACTGGGGC GGTGGCTGCA GACTGTGGGA AGGAATGGGA 
GATGAGACTT ACCCTCTAGC CAGACACGAT GATGGCTTAC TGGAAATCGT TGGCGTCTAT 
GGGTCTTTCC ACTGTGCTCA AATCCAGGTG AAACTGGCAA ATCCTTTTCG AATAGGACAA 
GCGCACACAG TGCGGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Diacylglycerol kinase ? deficiency preserves glucose tolerance and modulates lipid metabolism in obese mice.
Journal of lipid research58(5)907-915(2017 May)
Manner?s-Holm L,Sch?nke M,Brozinick JT,Vetterli L,Bui HH,Sanders P,Nascimento EBM,Bj?rnholm M,Chibalin AV,Zierath JR

Diacylglycerol kinase epsilon suppresses expression of p53 and glycerol kinase in mouse embryo fibroblasts.
Biochimica et biophysica acta1861(12 Pt A)1993-1999(2016 12)
So V,Jalan D,Lemaire M,Topham MK,Hatch GM,Epand RM

Loss of diacylglycerol kinase epsilon in mice causes endothelial distress and impairs glomerular Cox-2 and PGE2 production.
American journal of physiology. Renal physiology310(9)F895-908(2016 May)
Zhu J,Chaki M,Lu D,Ren C,Wang SS,Rauhauser A,Li B,Zimmerman S,Jun B,Du Y,Vadnagara K,Wang H,Elhadi S,Quigg RJ,Topham MK,Mohan C,Ozaltin F,Zhou XJ,Marciano DK,Bazan NG,Attanasio M

Diacylglycerol Kinase-?: Properties and Biological Roles.
Frontiers in cell and developmental biology4112(2016)
Epand RM,So V,Jennings W,Khadka B,Gupta RS,Lemaire M

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Dgke cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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