DGKE cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	DGKE
Entrez Gene ID	538147
Full Name	diacylglycerol kinase epsilon
General protein information	Preferred Names diacylglycerol kinase epsilon Names diacylglycerol kinase epsilon diacylglycerol kinase, epsilon 64kDa
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001192930.1	NP_001179859.1	diacylglycerol kinase epsilon
XM_005219865.4	XP_005219922.2	diacylglycerol kinase epsilon isoform X1
NM_001192930.3	NP_001179859.3	diacylglycerol kinase epsilon
XM_005219865.3	XP_005219922.1	diacylglycerol kinase epsilon isoform X1
XM_010815687.2	XP_010813989.1	diacylglycerol kinase epsilon isoform X2
XM_010815687.3	XP_010813989.2	diacylglycerol kinase epsilon isoform X2
NM_001192930.2	NP_001179859.2	diacylglycerol kinase epsilon

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DGKE	XP_548222.2
Arabidopsis thaliana (thale cress)	DGK1	NP_196409.1
Gallus gallus (chicken)	DGKE	XP_001234226.3
Mus musculus (house mouse)	Dgke	NP_062378.1
Danio rerio (zebrafish)	dgke	NP_001165699.1
Drosophila melanogaster (fruit fly)	Dgkepsilon	NP_725228.1
Caenorhabditis elegans (roundworm)	dgk-2	NP_001024679.1
Xenopus tropicalis (tropical clawed frog)	dgke	XP_002937855.1
Pan troglodytes (chimpanzee)	DGKE	XP_001171847.2
Homo sapiens (human)	DGKE	NP_003638.1
Bos taurus (cattle)	DGKE	NP_001179859.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006768	XP_308977.4
Oryza sativa (rice)	Os12g0576900	NP_001067111.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DGKE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa01668	NM_001192930.3 Latest version!	Bos taurus diacylglycerol kinase epsilon (DGKE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa29372	NM_001192930.2 Latest version!	Bos taurus diacylglycerol kinase epsilon (DGKE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa01668	XM_005219865.4 Latest version!	Bos taurus diacylglycerol kinase epsilon (DGKE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa29372	XM_005219865.3 Latest version!	Bos taurus diacylglycerol kinase epsilon (DGKE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa53833	XM_010815687.2 Latest version!	Bos taurus diacylglycerol kinase epsilon (DGKE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa226465	XM_010815687.3 Latest version!	Bos taurus diacylglycerol kinase epsilon (DGKE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OBa01668	NM_001192930.1	Bos taurus diacylglycerol kinase, epsilon 64kDa (DGKE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DGKE ( NM_001192930.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179859.1 Bos taurus diacylglycerol kinase, epsilon 64kDa (DGKE), mRNA.

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CloneID	OBa01668
Clone ID Related Accession (Same CDS sequence)	NM_001192930.1 , XM_005219865.4 , NM_001192930.3
Accession Version	NM_001192930.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2011-10-23
Organism	Bos taurus(Cattle)
Product	diacylglycerol kinase epsilon
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03078107.1, AAFC03078167.1 and AAFC03124418.1. On Jul 17, 2010 this sequence version replaced XM_618342.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG 
CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC 
GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG 
GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC 
AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG 
CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA 
CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA 
CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA 
AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA 
ACAGATTATG AAATGCTAGC CTCTAAACTT GGAAAGCAGT GGACTCCATT AATAATCCTG 
GCCAACTCTC GTAGTGGGAC TAACATGGGA GAAGGACTGC TGGGAGAGTT CAGGATCCTC 
CTAAATCCCG TCCAGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT 
TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC 
GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA 
CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT 
ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT 
GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA 
AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC 
CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT 
AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC 
ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA 
GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT 
GGGTCTTTCC ACTGTGCTCA GATTCAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA 
GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG 
CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG 
TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA 
AAGGAAACGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179859.1
CDS	1..1695
Translation	MEGQERPAPPASLFADGHLVLWTLCSVLLPVFFTFWCSVQRSRRQLHRRDIFRKSKHGWRDTDLFSQPTYCCVCAQHILQGAFCDCCGLRVDEGCLKKADKRFPCKEIMLKSDSKAVDAMPHHWIRGNVPLCSYCVVCKQQCGNQPKLCDYRCVWCQKTVHDECMKNSLRNEKCDFGEFKNLIIPPSYLTSINHMRKDKKTDYEMLASKLGKQWTPLIILANSRSGTNMGEGLLGEFRILLNPVQVFDVTKTPPTKALQLCTLLPYDSARVLVCGGDGTVGWVLDALDEMKIKGQEKYVPRVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGVKLDRWKVQVTNKGYYNLRKPKEFTMNNYFSIGPDALMALNFHAHREKAPSLFSSRILNKAVYLFYGTKDCLVQECKDLNKKVELELDGERVELPNLEGIIVLNIGYWGGGCRLWEGMGDETYPLARHDDGLLEVVGVYGSFHCAQIQVKLANPFRIGQAHTVRLILKCSMMPMQVDGEPWAQGPCTVTITHKTHALMLYFSGEQTDDDVSSTSDQEDTKETE

Target ORF information:

RefSeq Version	NM_001192930.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus diacylglycerol kinase, epsilon 64kDa (DGKE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192930.1

ORF Insert Sequence:

ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG 
CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC 
GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG 
GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC 
AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG 
CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA 
CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA 
CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA 
AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA 
ACAGATTATG AAATGCTAGC CTCTAAACTT GGAAAGCAGT GGACTCCATT AATAATCCTG 
GCCAACTCTC GTAGTGGGAC TAACATGGGA GAAGGACTGC TGGGAGAGTT CAGGATCCTC 
CTAAATCCCG TCCAGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT 
TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC 
GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA 
CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT 
ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT 
GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA 
AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC 
CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT 
AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC 
ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA 
GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT 
GGGTCTTTCC ACTGTGCTCA GATTCAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA 
GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG 
CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG 
TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA 
AAGGAAACGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKE ( NM_001192930.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179859.3 Bos taurus diacylglycerol kinase epsilon (DGKE), mRNA.

Price & Availability↑

CloneID	OBa01668
Clone ID Related Accession (Same CDS sequence)	NM_001192930.1 , XM_005219865.4 , NM_001192930.3
Accession Version	NM_001192930.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase epsilon
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000019.1. On May 29, 2018 this sequence version replaced NM_001192930.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571254.30610.1, SRR5571254.16311.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145432, SAMN03145443 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG 
CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC 
GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG 
GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC 
AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG 
CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA 
CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA 
CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA 
AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA 
ACAGATTATG AAATGCTAGC CTCTAAACTT GGAAAGCAGT GGACTCCATT AATAATCCTG 
GCCAACTCTC GTAGTGGGAC TAACATGGGA GAAGGACTGC TGGGAGAGTT CAGGATCCTC 
CTAAATCCCG TCCAGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT 
TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC 
GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA 
CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT 
ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT 
GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA 
AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC 
CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT 
AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC 
ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA 
GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT 
GGGTCTTTCC ACTGTGCTCA GATTCAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA 
GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG 
CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG 
TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA 
AAGGAAACGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179859.3
CDS	1..1695
Translation	MEGQERPAPPASLFADGHLVLWTLCSVLLPVFFTFWCSVQRSRRQLHRRDIFRKSKHGWRDTDLFSQPTYCCVCAQHILQGAFCDCCGLRVDEGCLKKADKRFPCKEIMLKSDSKAVDAMPHHWIRGNVPLCSYCVVCKQQCGNQPKLCDYRCVWCQKTVHDECMKNSLRNEKCDFGEFKNLIIPPSYLTSINHMRKDKKTDYEMLASKLGKQWTPLIILANSRSGTNMGEGLLGEFRILLNPVQVFDVTKTPPTKALQLCTLLPYDSARVLVCGGDGTVGWVLDALDEMKIKGQEKYVPRVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGVKLDRWKVQVTNKGYYNLRKPKEFTMNNYFSIGPDALMALNFHAHREKAPSLFSSRILNKAVYLFYGTKDCLVQECKDLNKKVELELDGERVELPNLEGIIVLNIGYWGGGCRLWEGMGDETYPLARHDDGLLEVVGVYGSFHCAQIQVKLANPFRIGQAHTVRLILKCSMMPMQVDGEPWAQGPCTVTITHKTHALMLYFSGEQTDDDVSSTSDQEDTKETE

Target ORF information:

RefSeq Version	NM_001192930.3
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase epsilon (DGKE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192930.3

ORF Insert Sequence:

ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG 
CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC 
GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG 
GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC 
AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG 
CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA 
CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA 
CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA 
AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA 
ACAGATTATG AAATGCTAGC CTCTAAACTT GGAAAGCAGT GGACTCCATT AATAATCCTG 
GCCAACTCTC GTAGTGGGAC TAACATGGGA GAAGGACTGC TGGGAGAGTT CAGGATCCTC 
CTAAATCCCG TCCAGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT 
TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC 
GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA 
CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT 
ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT 
GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA 
AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC 
CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT 
AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC 
ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA 
GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT 
GGGTCTTTCC ACTGTGCTCA GATTCAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA 
GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG 
CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG 
TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA 
AAGGAAACGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKE ( NM_001192930.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179859.2 Bos taurus diacylglycerol kinase epsilon (DGKE), mRNA.

Price & Availability↑

CloneID	OBa29372
Clone ID Related Accession (Same CDS sequence)	XM_005219865.3 , NM_001192930.2
Accession Version	NM_001192930.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase epsilon
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02048261.1. On Jun 30, 2017 this sequence version replaced NM_001192930.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02403653, SAMN02822088 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG 
CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC 
GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG 
GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC 
AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG 
CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA 
CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA 
CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA 
AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA 
ACAGATTATG AAATGCTAGC CTCTAAACTT GGAAAGCAGT GGACTCCATT AATAATCCTG 
GCCAACTCTC GTAGTGGGAC TAACATGGGA GAAGGACTGC TGGGAGAGTT CAGGATCCTC 
CTAAATCCCG TCCAGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT 
TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC 
GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA 
CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT 
ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT 
GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA 
AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC 
CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT 
AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC 
ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA 
GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT 
GGGTCTTTCC ACTGTGCTCA GAATAAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA 
GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG 
CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG 
TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA 
AAGGAAACGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179859.2
CDS	1..1695
Translation	MEGQERPAPPASLFADGHLVLWTLCSVLLPVFFTFWCSVQRSRRQLHRRDIFRKSKHGWRDTDLFSQPTYCCVCAQHILQGAFCDCCGLRVDEGCLKKADKRFPCKEIMLKSDSKAVDAMPHHWIRGNVPLCSYCVVCKQQCGNQPKLCDYRCVWCQKTVHDECMKNSLRNEKCDFGEFKNLIIPPSYLTSINHMRKDKKTDYEMLASKLGKQWTPLIILANSRSGTNMGEGLLGEFRILLNPVQVFDVTKTPPTKALQLCTLLPYDSARVLVCGGDGTVGWVLDALDEMKIKGQEKYVPRVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGVKLDRWKVQVTNKGYYNLRKPKEFTMNNYFSIGPDALMALNFHAHREKAPSLFSSRILNKAVYLFYGTKDCLVQECKDLNKKVELELDGERVELPNLEGIIVLNIGYWGGGCRLWEGMGDETYPLARHDDGLLEVVGVYGSFHCAQNKVKLANPFRIGQAHTVRLILKCSMMPMQVDGEPWAQGPCTVTITHKTHALMLYFSGEQTDDDVSSTSDQEDTKETE

Target ORF information:

RefSeq Version	NM_001192930.2
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase epsilon (DGKE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192930.2

ORF Insert Sequence:

ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG 
CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC 
GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG 
GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC 
AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG 
CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA 
CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA 
CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA 
AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA 
ACAGATTATG AAATGCTAGC CTCTAAACTT GGAAAGCAGT GGACTCCATT AATAATCCTG 
GCCAACTCTC GTAGTGGGAC TAACATGGGA GAAGGACTGC TGGGAGAGTT CAGGATCCTC 
CTAAATCCCG TCCAGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT 
TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC 
GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA 
CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT 
ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT 
GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA 
AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC 
CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT 
AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC 
ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA 
GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT 
GGGTCTTTCC ACTGTGCTCA GAATAAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA 
GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG 
CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG 
TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA 
AAGGAAACGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKE ( XM_005219865.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005219922.2 Bos taurus diacylglycerol kinase epsilon (DGKE), transcript variant X1, mRNA.

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CloneID	OBa01668
Clone ID Related Accession (Same CDS sequence)	NM_001192930.1 , XM_005219865.4 , NM_001192930.3
Accession Version	XM_005219865.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase epsilon isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005219865.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG 
CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC 
GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG 
GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC 
AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG 
CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA 
CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA 
CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA 
AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA 
ACAGATTATG AAATGCTAGC CTCTAAACTT GGAAAGCAGT GGACTCCATT AATAATCCTG 
GCCAACTCTC GTAGTGGGAC TAACATGGGA GAAGGACTGC TGGGAGAGTT CAGGATCCTC 
CTAAATCCCG TCCAGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT 
TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC 
GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA 
CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT 
ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT 
GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA 
AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC 
CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT 
AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC 
ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA 
GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT 
GGGTCTTTCC ACTGTGCTCA GATTCAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA 
GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG 
CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG 
TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA 
AAGGAAACGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005219922.2
CDS	228..1922
Translation	MEGQERPAPPASLFADGHLVLWTLCSVLLPVFFTFWCSVQRSRRQLHRRDIFRKSKHGWRDTDLFSQPTYCCVCAQHILQGAFCDCCGLRVDEGCLKKADKRFPCKEIMLKSDSKAVDAMPHHWIRGNVPLCSYCVVCKQQCGNQPKLCDYRCVWCQKTVHDECMKNSLRNEKCDFGEFKNLIIPPSYLTSINHMRKDKKTDYEMLASKLGKQWTPLIILANSRSGTNMGEGLLGEFRILLNPVQVFDVTKTPPTKALQLCTLLPYDSARVLVCGGDGTVGWVLDALDEMKIKGQEKYVPRVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGVKLDRWKVQVTNKGYYNLRKPKEFTMNNYFSIGPDALMALNFHAHREKAPSLFSSRILNKAVYLFYGTKDCLVQECKDLNKKVELELDGERVELPNLEGIIVLNIGYWGGGCRLWEGMGDETYPLARHDDGLLEVVGVYGSFHCAQIQVKLANPFRIGQAHTVRLILKCSMMPMQVDGEPWAQGPCTVTITHKTHALMLYFSGEQTDDDVSSTSDQEDTKETE

Target ORF information:

RefSeq Version	XM_005219865.4
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase epsilon (DGKE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005219865.4

ORF Insert Sequence:

ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG 
CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC 
GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG 
GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC 
AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG 
CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA 
CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA 
CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA 
AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA 
ACAGATTATG AAATGCTAGC CTCTAAACTT GGAAAGCAGT GGACTCCATT AATAATCCTG 
GCCAACTCTC GTAGTGGGAC TAACATGGGA GAAGGACTGC TGGGAGAGTT CAGGATCCTC 
CTAAATCCCG TCCAGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT 
TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC 
GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA 
CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT 
ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT 
GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA 
AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC 
CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT 
AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC 
ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA 
GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT 
GGGTCTTTCC ACTGTGCTCA GATTCAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA 
GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG 
CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG 
TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA 
AAGGAAACGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKE ( XM_005219865.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005219922.1 Bos taurus diacylglycerol kinase epsilon (DGKE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa29372
Clone ID Related Accession (Same CDS sequence)	XM_005219865.3 , NM_001192930.2
Accession Version	XM_005219865.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase epsilon isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104487.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005219865.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG 
CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC 
GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG 
GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC 
AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG 
CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA 
CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA 
CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA 
AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA 
ACAGATTATG AAATGCTAGC CTCTAAACTT GGAAAGCAGT GGACTCCATT AATAATCCTG 
GCCAACTCTC GTAGTGGGAC TAACATGGGA GAAGGACTGC TGGGAGAGTT CAGGATCCTC 
CTAAATCCCG TCCAGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT 
TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC 
GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA 
CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT 
ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT 
GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA 
AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC 
CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT 
AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC 
ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA 
GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT 
GGGTCTTTCC ACTGTGCTCA GAATAAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA 
GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG 
CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG 
TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA 
AAGGAAACGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005219922.1
CDS	225..1919
Translation	MEGQERPAPPASLFADGHLVLWTLCSVLLPVFFTFWCSVQRSRRQLHRRDIFRKSKHGWRDTDLFSQPTYCCVCAQHILQGAFCDCCGLRVDEGCLKKADKRFPCKEIMLKSDSKAVDAMPHHWIRGNVPLCSYCVVCKQQCGNQPKLCDYRCVWCQKTVHDECMKNSLRNEKCDFGEFKNLIIPPSYLTSINHMRKDKKTDYEMLASKLGKQWTPLIILANSRSGTNMGEGLLGEFRILLNPVQVFDVTKTPPTKALQLCTLLPYDSARVLVCGGDGTVGWVLDALDEMKIKGQEKYVPRVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGVKLDRWKVQVTNKGYYNLRKPKEFTMNNYFSIGPDALMALNFHAHREKAPSLFSSRILNKAVYLFYGTKDCLVQECKDLNKKVELELDGERVELPNLEGIIVLNIGYWGGGCRLWEGMGDETYPLARHDDGLLEVVGVYGSFHCAQNKVKLANPFRIGQAHTVRLILKCSMMPMQVDGEPWAQGPCTVTITHKTHALMLYFSGEQTDDDVSSTSDQEDTKETE

Target ORF information:

RefSeq Version	XM_005219865.3
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase epsilon (DGKE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005219865.3

ORF Insert Sequence:

ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG 
CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC 
GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG 
GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC 
AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG 
CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA 
CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA 
CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA 
AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA 
ACAGATTATG AAATGCTAGC CTCTAAACTT GGAAAGCAGT GGACTCCATT AATAATCCTG 
GCCAACTCTC GTAGTGGGAC TAACATGGGA GAAGGACTGC TGGGAGAGTT CAGGATCCTC 
CTAAATCCCG TCCAGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT 
TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC 
GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA 
CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT 
ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT 
GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA 
AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC 
CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT 
AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC 
ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA 
GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT 
GGGTCTTTCC ACTGTGCTCA GAATAAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA 
GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG 
CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG 
TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA 
AAGGAAACGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKE ( XM_010815687.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010813989.1 Bos taurus diacylglycerol kinase epsilon (DGKE), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa53833
Clone ID Related Accession (Same CDS sequence)	XM_010815687.2
Accession Version	XM_010815687.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase epsilon isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104487.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815687.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG 
CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC 
GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG 
GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC 
AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG 
CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA 
CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA 
CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA 
AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA 
ACAGATTATG AAATGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT 
TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC 
GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA 
CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT 
ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT 
GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA 
AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC 
CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT 
AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC 
ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA 
GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT 
GGGTCTTTCC ACTGTGCTCA GAATAAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA 
GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG 
CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG 
TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA 
AAGGAAACGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010813989.1
CDS	226..1800
Translation	MEGQERPAPPASLFADGHLVLWTLCSVLLPVFFTFWCSVQRSRRQLHRRDIFRKSKHGWRDTDLFSQPTYCCVCAQHILQGAFCDCCGLRVDEGCLKKADKRFPCKEIMLKSDSKAVDAMPHHWIRGNVPLCSYCVVCKQQCGNQPKLCDYRCVWCQKTVHDECMKNSLRNEKCDFGEFKNLIIPPSYLTSINHMRKDKKTDYEMVFDVTKTPPTKALQLCTLLPYDSARVLVCGGDGTVGWVLDALDEMKIKGQEKYVPRVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGVKLDRWKVQVTNKGYYNLRKPKEFTMNNYFSIGPDALMALNFHAHREKAPSLFSSRILNKAVYLFYGTKDCLVQECKDLNKKVELELDGERVELPNLEGIIVLNIGYWGGGCRLWEGMGDETYPLARHDDGLLEVVGVYGSFHCAQNKVKLANPFRIGQAHTVRLILKCSMMPMQVDGEPWAQGPCTVTITHKTHALMLYFSGEQTDDDVSSTSDQEDTKETE

Target ORF information:

RefSeq Version	XM_010815687.2
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase epsilon (DGKE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815687.2

ORF Insert Sequence:

ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG 
CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC 
GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG 
GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC 
AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG 
CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA 
CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA 
CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA 
AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA 
ACAGATTATG AAATGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT 
TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC 
GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA 
CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT 
ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT 
GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA 
AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC 
CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT 
AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC 
ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA 
GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT 
GGGTCTTTCC ACTGTGCTCA GAATAAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA 
GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG 
CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG 
TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA 
AAGGAAACGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKE ( XM_010815687.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010813989.2 Bos taurus diacylglycerol kinase epsilon (DGKE), transcript variant X2, mRNA.

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CloneID	OBa226465
Clone ID Related Accession (Same CDS sequence)	XM_010815687.3
Accession Version	XM_010815687.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase epsilon isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010815687.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG 
CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC 
GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG 
GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC 
AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG 
CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA 
CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA 
CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA 
AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA 
ACAGATTATG AAATGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT 
TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC 
GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA 
CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT 
ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT 
GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA 
AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC 
CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT 
AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC 
ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA 
GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT 
GGGTCTTTCC ACTGTGCTCA GATTCAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA 
GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG 
CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG 
TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA 
AAGGAAACGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010813989.2
CDS	229..1803
Translation	MEGQERPAPPASLFADGHLVLWTLCSVLLPVFFTFWCSVQRSRRQLHRRDIFRKSKHGWRDTDLFSQPTYCCVCAQHILQGAFCDCCGLRVDEGCLKKADKRFPCKEIMLKSDSKAVDAMPHHWIRGNVPLCSYCVVCKQQCGNQPKLCDYRCVWCQKTVHDECMKNSLRNEKCDFGEFKNLIIPPSYLTSINHMRKDKKTDYEMVFDVTKTPPTKALQLCTLLPYDSARVLVCGGDGTVGWVLDALDEMKIKGQEKYVPRVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGVKLDRWKVQVTNKGYYNLRKPKEFTMNNYFSIGPDALMALNFHAHREKAPSLFSSRILNKAVYLFYGTKDCLVQECKDLNKKVELELDGERVELPNLEGIIVLNIGYWGGGCRLWEGMGDETYPLARHDDGLLEVVGVYGSFHCAQIQVKLANPFRIGQAHTVRLILKCSMMPMQVDGEPWAQGPCTVTITHKTHALMLYFSGEQTDDDVSSTSDQEDTKETE

Target ORF information:

RefSeq Version	XM_010815687.3
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase epsilon (DGKE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815687.3

ORF Insert Sequence:

ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG 
CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC 
GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG 
GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC 
AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG 
CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA 
CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA 
CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA 
AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA 
ACAGATTATG AAATGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT 
TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC 
GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA 
CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT 
ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT 
GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA 
AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC 
CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT 
AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG 
AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC 
ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA 
GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT 
GGGTCTTTCC ACTGTGCTCA GATTCAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA 
GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG 
CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG 
TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA 
AAGGAAACGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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DGKE cDNA ORF clone, Bos taurus(cattle)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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