Ift20 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ift20
Entrez Gene ID	55978
Full Name	intraflagellar transport 20
Synonyms	0610009H04Rik,AU015496
General protein information	Preferred Names intraflagellar transport 20 Names intraflagellar transport protein 20 homolog intraflagellar transport 20 homolog mIFT20 uterine protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_018854.5	NP_061342.1	intraflagellar transport protein 20 homolog
NM_018854.4	NP_061342.1	intraflagellar transport protein 20 homolog

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	IFT20	XP_415917.1
Pan troglodytes (chimpanzee)	IFT20	XP_003953143.1
	LOC715773	XP_002800380.1
Rattus norvegicus (Norway rat)	Ift20	NP_001099285.1
Danio rerio (zebrafish)	ift20	NP_001001833.2
Canis lupus familiaris (dog)	IFT20	XP_003435353.1
Bos taurus (cattle)	IFT20	NP_001013621.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006172	XP_001688809.1
Homo sapiens (human)	IFT20	NP_001254703.1
Drosophila melanogaster (fruit fly)	CG30441	NP_724409.3
Mus musculus (house mouse)	Ift20	NP_061342.1
Xenopus tropicalis (tropical clawed frog)	ift20	NP_001007902.1

Gene Ontology
Bibliography

Related articles in PubMed

IFT20 modulates ciliary PDGFR? signaling by regulating the stability of Cbl E3 ubiquitin ligases.
Schmid FM, Schou KB, Vilhelm MJ, Holm MS, Breslin L, Farinelli P, Larsen LA, Andersen JS, Pedersen LB, Christensen ST
The Journal of cell biology217(1)151-161(2018 Jan)

Constitutively-active FGFR3 disrupts primary cilium length and IFT20 trafficking in various chondrocyte models of achondroplasia.
Martin L, Kaci N, Estibals V, Goudin N, Garfa-Traore M, Benoist-Lasselin C, Dambroise E, Legeai-Mallet L
Human molecular genetics27(1)1-13(2018 Jan)

Inhibition of Ciliogenesis Promotes Hedgehog Signaling, Tumorigenesis, and Metastasis in Breast Cancer.
Hassounah NB, Nunez M, Fordyce C, Roe D, Nagle R, Bunch T, McDermott KM
Molecular cancer research : MCR15(10)1421-1430(2017 Oct)

Intraflagellar transport protein IFT20 is essential for male fertility and spermiogenesis in mice.
Zhang Z, Li W, Zhang Y, Zhang L, Teves ME, Liu H, Strauss JF 3rd, Pazour GJ, Foster JA, Hess RA, Zhang Z
Molecular biology of the cell(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

IFT20 is required for the delivery of the intracellular pool of LAT to the immune synapse in naive primary T lymphocytes.
Title: IFT20 controls LAT recruitment to the immune synapse and T-cell activation in vivo.

Ciliary proteins Bbs8 and Ift20 promote planar cell polarity in the cochlea.
Title: Ciliary proteins Bbs8 and Ift20 promote planar cell polarity in the cochlea.

This work is a strong support of a model of opsin transport where IFT20 is involved in the movement from the Golgi complex to the base of the cilium
Title: Distinct functions for IFT140 and IFT20 in opsin transport.

Mxi1 regulates Ift20 promoter activity via Ets-1 binding to the Ift20 promoter. These results indicate that inactivating Mxi1 induces ciliary defects in polycystic kidney.
Title: Inactivation of max-interacting protein 1 induces renal cilia disassembly through reduction in levels of intraflagellar transport 20 in polycystic kidney.

Data show that loss of IFT20 during cone development leads to opsin accumulation in the inner segment even when the connecting cilium and outer segment are still intact.
Title: IFT20 is required for opsin trafficking and photoreceptor outer segment development.

The following Ift20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ift20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu17934	NM_018854.5 Latest version!	Mus musculus intraflagellar transport 20 (Ift20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OMu17934	NM_018854.4	Mus musculus intraflagellar transport 20 (Ift20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ift20 ( NM_018854.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_061342.1 Mus musculus intraflagellar transport 20 (Ift20), mRNA.

Price & Availability↑

CloneID	OMu17934
Clone ID Related Accession (Same CDS sequence)	NM_018854.5 , NM_018854.4
Accession Version	NM_018854.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Mus musculus(house mouse)
Product	intraflagellar transport protein 20 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591177.14. On Mar 1, 2018 this sequence version replaced NM_018854.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK139942.1, DV063541.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAGG ACATCTTGGG CGAGGCAGGG CTGCATTTTG ATGAACTGAA CAAGCTCCGA 
GTGTTGGACC CAGAGGTCAC CCAGCAGACT GTGGAGCTCA AGGAGGAGTG CAAGGACTTT 
GTGGACAAAA TTGGCCAGTT TCAGAAAATT GTTGGTGGTC TAATTGAGCT TGTTGATCAG 
CTTGCCAAAG AAGCAGAGAA CGAGAAGATG AAGGCCATTG GTGCTCGGAA CTTGCTGAAG 
TCCATAGCGA AGCAGAGAGA AGCCCAGCAG CAGCAGCTGC AGGCCCTGAT AGCAGAAAAG 
AAGACGCAGC TAGAAAGGTA TCGGGTTGAA TATGAAGCTT TGTGTAAAGT AGAAGCAGAA 
CAAAATGAAT TTATTGACCA ATTTATTTTT CAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061342.1
CDS	448..846
Translation	MAKDILGEAGLHFDELNKLRVLDPEVTQQTVELKEECKDFVDKIGQFQKIVGGLIELVDQLAKEAENEKMKAIGARNLLKSIAKQREAQQQQLQALIAEKKTQLERYRVEYEALCKVEAEQNEFIDQFIFQK

Target ORF information:

RefSeq Version	NM_018854.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus intraflagellar transport 20 (Ift20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018854.5

ORF Insert Sequence:

ATGGCCAAGG ACATCTTGGG CGAGGCAGGG CTGCATTTTG ATGAACTGAA CAAGCTCCGA 
GTGTTGGACC CAGAGGTCAC CCAGCAGACT GTGGAGCTCA AGGAGGAGTG CAAGGACTTT 
GTGGACAAAA TTGGCCAGTT TCAGAAAATT GTTGGTGGTC TAATTGAGCT TGTTGATCAG 
CTTGCCAAAG AAGCAGAGAA CGAGAAGATG AAGGCCATTG GTGCTCGGAA CTTGCTGAAG 
TCCATAGCGA AGCAGAGAGA AGCCCAGCAG CAGCAGCTGC AGGCCCTGAT AGCAGAAAAG 
AAGACGCAGC TAGAAAGGTA TCGGGTTGAA TATGAAGCTT TGTGTAAAGT AGAAGCAGAA 
CAAAATGAAT TTATTGACCA ATTTATTTTT CAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ift20 ( NM_018854.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_061342.1 Mus musculus intraflagellar transport 20 (Ift20), mRNA.

Price & Availability↑

CloneID	OMu17934
Clone ID Related Accession (Same CDS sequence)	NM_018854.5 , NM_018854.4
Accession Version	NM_018854.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Mus musculus(house mouse)
Product	intraflagellar transport protein 20 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK002397.1. On Apr 6, 2007 this sequence version replaced NM_018854.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK002397.1, AK139942.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAAGG ACATCTTGGG CGAGGCAGGG CTGCATTTTG ATGAACTGAA CAAGCTCCGA 
GTGTTGGACC CAGAGGTCAC CCAGCAGACT GTGGAGCTCA AGGAGGAGTG CAAGGACTTT 
GTGGACAAAA TTGGCCAGTT TCAGAAAATT GTTGGTGGTC TAATTGAGCT TGTTGATCAG 
CTTGCCAAAG AAGCAGAGAA CGAGAAGATG AAGGCCATTG GTGCTCGGAA CTTGCTGAAG 
TCCATAGCGA AGCAGAGAGA AGCCCAGCAG CAGCAGCTGC AGGCCCTGAT AGCAGAAAAG 
AAGACGCAGC TAGAAAGGTA TCGGGTTGAA TATGAAGCTT TGTGTAAAGT AGAAGCAGAA 
CAAAATGAAT TTATTGACCA ATTTATTTTT CAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061342.1
CDS	373..771
Translation	MAKDILGEAGLHFDELNKLRVLDPEVTQQTVELKEECKDFVDKIGQFQKIVGGLIELVDQLAKEAENEKMKAIGARNLLKSIAKQREAQQQQLQALIAEKKTQLERYRVEYEALCKVEAEQNEFIDQFIFQK

Target ORF information:

RefSeq Version	NM_018854.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus intraflagellar transport 20 (Ift20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018854.4

ORF Insert Sequence:

ATGGCCAAGG ACATCTTGGG CGAGGCAGGG CTGCATTTTG ATGAACTGAA CAAGCTCCGA 
GTGTTGGACC CAGAGGTCAC CCAGCAGACT GTGGAGCTCA AGGAGGAGTG CAAGGACTTT 
GTGGACAAAA TTGGCCAGTT TCAGAAAATT GTTGGTGGTC TAATTGAGCT TGTTGATCAG 
CTTGCCAAAG AAGCAGAGAA CGAGAAGATG AAGGCCATTG GTGCTCGGAA CTTGCTGAAG 
TCCATAGCGA AGCAGAGAGA AGCCCAGCAG CAGCAGCTGC AGGCCCTGAT AGCAGAAAAG 
AAGACGCAGC TAGAAAGGTA TCGGGTTGAA TATGAAGCTT TGTGTAAAGT AGAAGCAGAA 
CAAAATGAAT TTATTGACCA ATTTATTTTT CAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

IFT20 modulates ciliary PDGFR? signaling by regulating the stability of Cbl E3 ubiquitin ligases.
The Journal of cell biology217(1)151-161(2018 Jan)
Schmid FM,Schou KB,Vilhelm MJ,Holm MS,Breslin L,Farinelli P,Larsen LA,Andersen JS,Pedersen LB,Christensen ST

Constitutively-active FGFR3 disrupts primary cilium length and IFT20 trafficking in various chondrocyte models of achondroplasia.
Human molecular genetics27(1)1-13(2018 Jan)
Martin L,Kaci N,Estibals V,Goudin N,Garfa-Traore M,Benoist-Lasselin C,Dambroise E,Legeai-Mallet L

Inhibition of Ciliogenesis Promotes Hedgehog Signaling, Tumorigenesis, and Metastasis in Breast Cancer.
Molecular cancer research : MCR15(10)1421-1430(2017 Oct)
Hassounah NB,Nunez M,Fordyce C,Roe D,Nagle R,Bunch T,McDermott KM

Intraflagellar transport protein IFT20 is essential for male fertility and spermiogenesis in mice.
Molecular biology of the cell(2016 Sep)
Zhang Z,Li W,Zhang Y,Zhang L,Teves ME,Liu H,Strauss JF 3rd,Pazour GJ,Foster JA,Hess RA,Zhang Z

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Ift20 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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