Nus1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nus1
Entrez Gene ID	52014
Full Name	NUS1 dehydrodolichyl diphosphate synthase subunit
Synonyms	1600027K07Rik,AU019165,AW538011,BC003223,D10Ertd438e
General protein information	Preferred Names NUS1 dehydrodolichyl diphosphate synthase subunit Names dehydrodolichyl diphosphate synthase complex subunit Nus1 Nogo-B receptor dehydrodolichyl diphosphate syntase complex subunit Nus1 di-trans,poly-cis-decaprenylcistransferase ngBR nuclear undecaprenyl pyrophosphate synthase 1 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 B3\|10 26.64 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_030250.2	NP_084526.1	dehydrodolichyl diphosphate synthase complex subunit Nus1

Pathways from BioSystems

Homologies

	NUS1	XP_001110733.1
Bos taurus (cattle)	NUS1	NP_001242897.1
Homo sapiens (human)	NUS1	NP_612468.1
Mus musculus (house mouse)	Nus1	NP_084526.1
Canis lupus familiaris (dog)	NUS1	XP_541219.2
Rattus norvegicus (Norway rat)	Nus1	NP_001157629.1
Gallus gallus (chicken)	NUS1	XP_419766.3
Danio rerio (zebrafish)	nus1	NP_001002356.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000402	XP_565967.3
Pan troglodytes (chimpanzee)	NUS1	XP_518713.1

Gene Ontology
Bibliography

Related articles in PubMed

The Nogo-B receptor promotes Ras plasma membrane localization and activation.
Zhao B, Hu W, Kumar S, Gonyo P, Rana U, Liu Z, Wang B, Duong WQ, Yang Z, Williams CL, Miao QR
Oncogene36(24)3406-3416(2017 Jun)

Nogo-B receptor deficiency increases liver X receptor alpha nuclear translocation and hepatic lipogenesis through an adenosine monophosphate-activated protein kinase alpha-dependent pathway.
Hu W, Zhang W, Chen Y, Rana U, Teng RJ, Duan Y, Liu Z, Zhao B, Foeckler J, Weiler H, Kallinger RE, Thomas MJ, Zhang K, Han J, Miao QR
Hepatology (Baltimore, Md.)64(5)1559-1576(2016 11)

Nogo-B receptor deficiency causes cerebral vasculature defects during embryonic development in mice.
Rana U, Liu Z, Kumar SN, Zhao B, Hu W, Bordas M, Cossette S, Szabo S, Foeckler J, Weiler H, Chrzanowska-Wodnicka M, Holtz ML, Misra RP, Salato V, North PE, Ramchandran R, Miao QR
Developmental biology410(2)190-201(2016 Feb)

NgBR is essential for endothelial cell glycosylation and vascular development.
Park EJ, Grabi?ska KA, Guan Z, Sessa WC
EMBO reports17(2)167-77(2016 Feb)

Mutation of Nogo-B receptor, a subunit of cis-prenyltransferase, causes a congenital disorder of glycosylation.
Park EJ, Grabi?ska KA, Guan Z, Str?neck? V, Hartmannov? H, Hoda?ov? K, Bare?ov? V, Sovov? J, Jozsef L, Ondru?kov? N, Hans?kov? H, Honz?k T, Zeman J, H?lkov? H, Wen R, Kmoch S, Sessa WC
Cell metabolism20(3)448-57(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data demonstrate that NgBR is a unique receptor that promotes accumulation of prenylated Ras at the plasma membrane and promotes EGF pathways.
Title: The Nogo-B receptor promotes Ras plasma membrane localization and activation.

NgBR is critical for protein glycosylation during vascular development.
Title: NgBR is essential for endothelial cell glycosylation and vascular development.

Loss of Nogo-B receptor in mice causes early embryonic lethality and defects in glycosylation.
Title: Mutation of Nogo-B receptor, a subunit of cis-prenyltransferase, causes a congenital disorder of glycosylation.

Our results indicate that the NgBR ectodomain is intrinsically unstructured without tertiary structures while the cytoplasmic domain is partially folded with secondary structures but without a tight tertiary packing [nogo-b receptor]
Title: Nogo-B receptor possesses an intrinsically unstructured ectodomain and a partially folded cytoplasmic domain.

identify a previously uncharacterized Nogo-B receptor specific for the amino terminus of Nogo-B.
Title: Identification of a receptor necessary for Nogo-B stimulated chemotaxis and morphogenesis of endothelial cells.

The following Nus1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nus1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08243	NM_030250.2 Latest version!	Mus musculus NUS1 dehydrodolichyl diphosphate synthase subunit (Nus1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nus1 ( NM_030250.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_084526.1 Mus musculus NUS1 dehydrodolichyl diphosphate synthase subunit (Nus1), mRNA.

Price & Availability↑

CloneID	OMu08243
Clone ID Related Accession (Same CDS sequence)	NM_030250.2
Accession Version	NM_030250.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	dehydrodolichyl diphosphate synthase complex subunit Nus1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC153526.13. On Aug 13, 2009 this sequence version replaced NM_030250.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.53176.1, SRR1660819.86647.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGGGC TGTACGAGCT GGTGTGGCGG GTGCTGCACG CGCTGCTCTG CCTGCACCTC 
ACGCTCACCT CCTGGCTCCG CGTTCGCTTC GGCACCTGGA ACTGGATCTG GCGGCGCTGC 
TGTCGCGCCG CCTCCGCCGC GGTCCTAGCG CCGCTCGGCT TCACGCTCCG CAAGCCCCGG 
GCCGTCGGCA GGAACCGGCG TCATCACCGG CACCCGCACG GGGGACCGGG ACCGGGACCG 
GGACCGGCCG CCACCCATCC TCGGCTGCGC TGGCGCGCGG ACGTCCGGTC CCTGCAGAAG 
CTGCCGGTGC ACATGGGCCT GTTGGTCACG GAGGAAGTGC AGGAGCCCAG CTTCTCAGAC 
ATCGCCAGCC TCGTGGTGTG GTGTATGGCC GTGGGGATCT CCTACATTAG CGTCTACGAC 
CACCAAGGTA TTTTCAAGAG AAATAATTCC AGATTGATGG ATGAAATTTT AAAACAGCAA 
CAGGAACTTT TGGGCCAAGA TTGTTCCAAA TACTCAGCAG AGTTTGCAAA TAGTAATGAC 
AAAGACGATC AAGATTTAAA TTGCCCTTCG GCAGTGAAGG TGCTGTCCCC AGAAGATGGA 
AAAGCGGATA TTGTGAGAGC TGCCCAGGAT TTTTGCCAGT TAGTAGCCCA GCAGCAGAGG 
AAGCCCACAG ATCTGGATGT AGATCTGCTA GGCAGCTTAC TTAGTTCACA TGGGTTCCCT 
GATCCTGACT TAGTGTTGAA GTTTGGTCCT GTGGACAGCA CATTAGGCTT TCTTCCCTGG 
CAAATCAGAT TGACTGAGAT CGTCTCTCTG CCTTCTCACC TAAACATCAG CTATGAGGAC 
TTCTTCTCCG CCCTTCGGCA GTATGCAGCT TGCGAACAGC GACTGGGAAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_084526.1
CDS	196..1089
Translation	MTGLYELVWRVLHALLCLHLTLTSWLRVRFGTWNWIWRRCCRAASAAVLAPLGFTLRKPRAVGRNRRHHRHPHGGPGPGPGPAATHPRLRWRADVRSLQKLPVHMGLLVTEEVQEPSFSDIASLVVWCMAVGISYISVYDHQGIFKRNNSRLMDEILKQQQELLGQDCSKYSAEFANSNDKDDQDLNCPSAVKVLSPEDGKADIVRAAQDFCQLVAQQQRKPTDLDVDLLGSLLSSHGFPDPDLVLKFGPVDSTLGFLPWQIRLTEIVSLPSHLNISYEDFFSALRQYAACEQRLGK

Target ORF information:

RefSeq Version	NM_030250.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NUS1 dehydrodolichyl diphosphate synthase subunit (Nus1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030250.2

ORF Insert Sequence:

ATGACGGGGC TGTACGAGCT GGTGTGGCGG GTGCTGCACG CGCTGCTCTG CCTGCACCTC 
ACGCTCACCT CCTGGCTCCG CGTTCGCTTC GGCACCTGGA ACTGGATCTG GCGGCGCTGC 
TGTCGCGCCG CCTCCGCCGC GGTCCTAGCG CCGCTCGGCT TCACGCTCCG CAAGCCCCGG 
GCCGTCGGCA GGAACCGGCG TCATCACCGG CACCCGCACG GGGGACCGGG ACCGGGACCG 
GGACCGGCCG CCACCCATCC TCGGCTGCGC TGGCGCGCGG ACGTCCGGTC CCTGCAGAAG 
CTGCCGGTGC ACATGGGCCT GTTGGTCACG GAGGAAGTGC AGGAGCCCAG CTTCTCAGAC 
ATCGCCAGCC TCGTGGTGTG GTGTATGGCC GTGGGGATCT CCTACATTAG CGTCTACGAC 
CACCAAGGTA TTTTCAAGAG AAATAATTCC AGATTGATGG ATGAAATTTT AAAACAGCAA 
CAGGAACTTT TGGGCCAAGA TTGTTCCAAA TACTCAGCAG AGTTTGCAAA TAGTAATGAC 
AAAGACGATC AAGATTTAAA TTGCCCTTCG GCAGTGAAGG TGCTGTCCCC AGAAGATGGA 
AAAGCGGATA TTGTGAGAGC TGCCCAGGAT TTTTGCCAGT TAGTAGCCCA GCAGCAGAGG 
AAGCCCACAG ATCTGGATGT AGATCTGCTA GGCAGCTTAC TTAGTTCACA TGGGTTCCCT 
GATCCTGACT TAGTGTTGAA GTTTGGTCCT GTGGACAGCA CATTAGGCTT TCTTCCCTGG 
CAAATCAGAT TGACTGAGAT CGTCTCTCTG CCTTCTCACC TAAACATCAG CTATGAGGAC 
TTCTTCTCCG CCCTTCGGCA GTATGCAGCT TGCGAACAGC GACTGGGAAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Nogo-B receptor promotes Ras plasma membrane localization and activation.
Oncogene36(24)3406-3416(2017 Jun)
Zhao B,Hu W,Kumar S,Gonyo P,Rana U,Liu Z,Wang B,Duong WQ,Yang Z,Williams CL,Miao QR

Nogo-B receptor deficiency increases liver X receptor alpha nuclear translocation and hepatic lipogenesis through an adenosine monophosphate-activated protein kinase alpha-dependent pathway.
Hepatology (Baltimore, Md.)64(5)1559-1576(2016 11)
Hu W,Zhang W,Chen Y,Rana U,Teng RJ,Duan Y,Liu Z,Zhao B,Foeckler J,Weiler H,Kallinger RE,Thomas MJ,Zhang K,Han J,Miao QR

Nogo-B receptor deficiency causes cerebral vasculature defects during embryonic development in mice.
Developmental biology410(2)190-201(2016 Feb)
Rana U,Liu Z,Kumar SN,Zhao B,Hu W,Bordas M,Cossette S,Szabo S,Foeckler J,Weiler H,Chrzanowska-Wodnicka M,Holtz ML,Misra RP,Salato V,North PE,Ramchandran R,Miao QR

NgBR is essential for endothelial cell glycosylation and vascular development.
EMBO reports17(2)167-77(2016 Feb)
Park EJ,Grabi?ska KA,Guan Z,Sessa WC

Mutation of Nogo-B receptor, a subunit of cis-prenyltransferase, causes a congenital disorder of glycosylation.
Cell metabolism20(3)448-57(2014 Sep)
Park EJ,Grabi?ska KA,Guan Z,Str?neck? V,Hartmannov? H,Hoda?ov? K,Bare?ov? V,Sovov? J,Jozsef L,Ondru?kov? N,Hans?kov? H,Honz?k T,Zeman J,H?lkov? H,Wen R,Kmoch S,Sessa WC

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Nus1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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