Nup85 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nup85
Entrez Gene ID	445007
Full Name	nucleoporin 85
Synonyms	Pcnt1,frount
General protein information	Preferred Names nucleoporin 85 Names nuclear pore complex protein Nup85 85 kDa nucleoporin nucleoporin Nup85 pericentrin-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 E2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006533671.2	XP_006533734.1	nuclear pore complex protein Nup85 isoform X2
NM_001002929.4	NP_001002929.3	nuclear pore complex protein Nup85
XM_006533671.3	XP_006533734.1	nuclear pore complex protein Nup85 isoform X2
XM_030246116.1	XP_030101976.1	nuclear pore complex protein Nup85 isoform X3
XM_006533670.3	XP_006533733.1	nuclear pore complex protein Nup85 isoform X1
XM_006533670.4	XP_006533733.1	nuclear pore complex protein Nup85 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NUP85	XP_003953232.1
Bos taurus (cattle)	NUP85	NP_001029559.1
Canis lupus familiaris (dog)	NUP85	XP_533121.2
Danio rerio (zebrafish)	nup85	NP_001003625.1
Caenorhabditis elegans (roundworm)	npp-2	NP_492256.1
Arabidopsis thaliana (thale cress)	AT4G32910	NP_567908.1
Oryza sativa (rice)	Os01g0746200	NP_001044228.1
Mus musculus (house mouse)	Nup85	NP_001002929.3
Gallus gallus (chicken)	NUP85	NP_001006426.1
Drosophila melanogaster (fruit fly)	Nup75	NP_611300.2
Homo sapiens (human)	NUP85	NP_079120.1
	LOC705628	XP_001093985.2
Rattus norvegicus (Norway rat)	Nup85	NP_001020572.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012362	XP_320194.4
Xenopus tropicalis (tropical clawed frog)	nup85	NP_001004817.1

Gene Ontology
Bibliography

Related articles in PubMed

Venus trap in the mouse embryo reveals distinct molecular dynamics underlying specification of first embryonic lineages.
Dietrich JE, Panavaite L, Gunther S, Wennekamp S, Groner AC, Pigge A, Salvenmoser S, Trono D, Hufnagel L, Hiiragi T
EMBO reports16(8)1005-21(2015 Aug)

Pivotal function for cytoplasmic protein FROUNT in CCR2-mediated monocyte chemotaxis.
Terashima Y, Onai N, Murai M, Enomoto M, Poonpiriya V, Hamada T, Motomura K, Suwa M, Ezaki T, Haga T, Kanegasaki S, Matsushima K
Nature immunology6(8)827-35(2005 Aug)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

FROUNT links activated CCR2 to the PI(3)K-Rac-lamellipodium protrusion cascade in monocyte chemotaxis
Title: Pivotal function for cytoplasmic protein FROUNT in CCR2-mediated monocyte chemotaxis.

The following Nup85 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nup85 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15655	NM_001002929.4 Latest version!	Mus musculus nucleoporin 85 (Nup85), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu46560	XM_006533670.4 Latest version!	Mus musculus nucleoporin 85 (Nup85), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu46561	XM_006533671.3 Latest version!	Mus musculus nucleoporin 85 (Nup85), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu85832	XM_030246116.1 Latest version!	Mus musculus nucleoporin 85 (Nup85), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu46561	XM_006533671.2	Mus musculus nucleoporin 85 (Nup85), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu46560	XM_006533670.3	Mus musculus nucleoporin 85 (Nup85), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nup85 ( XM_006533671.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533734.1 Mus musculus nucleoporin 85 (Nup85), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu46561
Clone ID Related Accession (Same CDS sequence)	XM_006533671.2 , XM_006533671.3
Accession Version	XM_006533671.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nuclear pore complex protein Nup85 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006533671.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTTTTG ACTGGGGCCC CGGCGAGATG CTTCTGTGTG AAACTTCCTT CAACCAAACA 
GGCAAATCAG AGAAGGTGCC GAGCTGCCCT TTTATCTACA TCATACGGAA AGATGTGGAT 
GTTTACTCTC AAATTTTGAG AAAACTCTTC AATGAATCCC ATGGAATCTT TGTTGGCCTG 
CAAAAAATTG AGGAAGAACT GTCGGGGAAG TCCAGGAAAG CTCAATTGGT TCGAGTGAGT 
AAAAATTACC GTTCAGTCAT ACGGGCCTGT ATGGAAGAAA TGCATCAGGT TGCAATTGCT 
GCTAAAGATC CAGCCAGTGG CCGGCAGTTC AGCAGCCAGG TCTCCATTTT GTCAGCCATG 
GAGCTCATTT GGAACCTGTG TGAGATTCTC TTTATTGAAG TAGCACCAGC TGGCCCTCTC 
CTCCTTCACC TTCTTGACTG GGTCCGACTG CACGTGTGCG AGGTGGACAG TTTGTCGGCA 
GATGTCCTGG GCGGTGACAA CCCAAGCAAG CATGAAAACT TCTGGGACCT GGTGACTGTT 
CTGGTGCTTC AGGGCCGGCT CGATGAGGCA CGGCAGATGC TAGCCAAAGA AGCTGATGCC 
AACCCCTCTT GTGCAGGCAT GTGCCGCGTC CTTGGGGACC TGATGAGGAC AATGCCCATT 
CTCAGCCCTG GCAATACTCA GACACTGACA GAGTTGGAGC TGAAGTGGCA GCACTGGCGT 
GAGGAGTGTG AAAGACACTT ACAAGACAAC ACATTTGCAG CCAACCCCCG TCTGGAGTCT 
CTCTGCAAGA TCATGCTGGG AGATGAGGCC GCCTTGTTGG AGCAGAAGGA GCTTCTGAGC 
AACTGGTACC ATTTCTTAGT GACGAGGCTG CTGTACTCTA ACCCCACAGT GAAGCCCATT 
GACCTGCACT TCTATGCCCA GTCCAGCCTA GACATGTTTC TTGGAGGTGA GAGCAGTCCA 
GAACCACTGG ACAACATCTT GATGGCGGCC TTTGAGTTCG ACATTCACCA GGTGATCAAA 
GAGTGCAGCA TCGCCCTGAG CAACTGGTGG TTCGTAGCTC ACCTCACAGA CCTTTTGGAT 
CACTGCAGAC TCCTCCAGTC ACACAATCTC TATTTTGGTT CTAACATGAG AGAATTCCTC 
CTGCTGGAGT ACGCCTCAGG ACTGTTTGCT CACCACAGCC TGTGGCAGCT GGGGGTGGAC 
TACTTTGATT ACTGCCCGGA GCTAGGCCGA GTTTCCTTGG AGCTGCACAT TGAGCGGATT 
CCTCTCAACA CAGAGCAGAA AGCCTTGAAG GTGCTGAGGA TTTGTGAGCA GCGGCAGATG 
ACTGAGCAAG TTAAAAGCAT CTGTAAGATC TTGGCCATGA AGGCTGTTCG TAATAACCGC 
TTGGGCTCAG CACTCTCCTG GAGCATCCGT GCCAAAGATG CTGCCTTTGC CACACTCGTA 
TCTGACAGAT TCCTCCGGGA TTACTGTGAG AGAGGCTGCT TTTCTGACTT GGATCTCATT 
GACAATCTGG GGTCAGCCAT GATGCTCAGT GATCGACTGA CGTTTCTGGG AAAGTACCGG 
GAGTTCCACA GACTGTACGG GGAGAAGCGC TTTGGTGACG CTGCTTCTCT TCTGCTGTCC 
CTCATGACCT CTCAGATTGC ACCTCGTTCT TTCTGGATGA CTCTGCTCAC AGATGCCCTT 
CCTCTTTTGG AACAGAAACA GGTGATTTTT TCAGCAGAGC AGACATATGA GCTGATGCGG 
TGCCTGGAAG ACTTGGCCTC AGGGAGGCCA GAGTGTGGTG AACCTGATGC CCAGCGACTG 
CAGGATGACG ACATAGAGAC CACCAAGGTG GAGATGCTGA GACTGGCTCT TGCCCGGAAT 
CTTGCTCGGG CAATTATAAG GGAAGGCTCA CTGGAGGGTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533734.1
CDS	107..2011
Translation	MCFDWGPGEMLLCETSFNQTGKSEKVPSCPFIYIIRKDVDVYSQILRKLFNESHGIFVGLQKIEEELSGKSRKAQLVRVSKNYRSVIRACMEEMHQVAIAAKDPASGRQFSSQVSILSAMELIWNLCEILFIEVAPAGPLLLHLLDWVRLHVCEVDSLSADVLGGDNPSKHENFWDLVTVLVLQGRLDEARQMLAKEADANPSCAGMCRVLGDLMRTMPILSPGNTQTLTELELKWQHWREECERHLQDNTFAANPRLESLCKIMLGDEAALLEQKELLSNWYHFLVTRLLYSNPTVKPIDLHFYAQSSLDMFLGGESSPEPLDNILMAAFEFDIHQVIKECSIALSNWWFVAHLTDLLDHCRLLQSHNLYFGSNMREFLLLEYASGLFAHHSLWQLGVDYFDYCPELGRVSLELHIERIPLNTEQKALKVLRICEQRQMTEQVKSICKILAMKAVRNNRLGSALSWSIRAKDAAFATLVSDRFLRDYCERGCFSDLDLIDNLGSAMMLSDRLTFLGKYREFHRLYGEKRFGDAASLLLSLMTSQIAPRSFWMTLLTDALPLLEQKQVIFSAEQTYELMRCLEDLASGRPECGEPDAQRLQDDDIETTKVEMLRLALARNLARAIIREGSLEGS

Target ORF information:

RefSeq Version	XM_006533671.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nucleoporin 85 (Nup85), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533671.2

ORF Insert Sequence:

ATGTGTTTTG ACTGGGGCCC CGGCGAGATG CTTCTGTGTG AAACTTCCTT CAACCAAACA 
GGCAAATCAG AGAAGGTGCC GAGCTGCCCT TTTATCTACA TCATACGGAA AGATGTGGAT 
GTTTACTCTC AAATTTTGAG AAAACTCTTC AATGAATCCC ATGGAATCTT TGTTGGCCTG 
CAAAAAATTG AGGAAGAACT GTCGGGGAAG TCCAGGAAAG CTCAATTGGT TCGAGTGAGT 
AAAAATTACC GTTCAGTCAT ACGGGCCTGT ATGGAAGAAA TGCATCAGGT TGCAATTGCT 
GCTAAAGATC CAGCCAGTGG CCGGCAGTTC AGCAGCCAGG TCTCCATTTT GTCAGCCATG 
GAGCTCATTT GGAACCTGTG TGAGATTCTC TTTATTGAAG TAGCACCAGC TGGCCCTCTC 
CTCCTTCACC TTCTTGACTG GGTCCGACTG CACGTGTGCG AGGTGGACAG TTTGTCGGCA 
GATGTCCTGG GCGGTGACAA CCCAAGCAAG CATGAAAACT TCTGGGACCT GGTGACTGTT 
CTGGTGCTTC AGGGCCGGCT CGATGAGGCA CGGCAGATGC TAGCCAAAGA AGCTGATGCC 
AACCCCTCTT GTGCAGGCAT GTGCCGCGTC CTTGGGGACC TGATGAGGAC AATGCCCATT 
CTCAGCCCTG GCAATACTCA GACACTGACA GAGTTGGAGC TGAAGTGGCA GCACTGGCGT 
GAGGAGTGTG AAAGACACTT ACAAGACAAC ACATTTGCAG CCAACCCCCG TCTGGAGTCT 
CTCTGCAAGA TCATGCTGGG AGATGAGGCC GCCTTGTTGG AGCAGAAGGA GCTTCTGAGC 
AACTGGTACC ATTTCTTAGT GACGAGGCTG CTGTACTCTA ACCCCACAGT GAAGCCCATT 
GACCTGCACT TCTATGCCCA GTCCAGCCTA GACATGTTTC TTGGAGGTGA GAGCAGTCCA 
GAACCACTGG ACAACATCTT GATGGCGGCC TTTGAGTTCG ACATTCACCA GGTGATCAAA 
GAGTGCAGCA TCGCCCTGAG CAACTGGTGG TTCGTAGCTC ACCTCACAGA CCTTTTGGAT 
CACTGCAGAC TCCTCCAGTC ACACAATCTC TATTTTGGTT CTAACATGAG AGAATTCCTC 
CTGCTGGAGT ACGCCTCAGG ACTGTTTGCT CACCACAGCC TGTGGCAGCT GGGGGTGGAC 
TACTTTGATT ACTGCCCGGA GCTAGGCCGA GTTTCCTTGG AGCTGCACAT TGAGCGGATT 
CCTCTCAACA CAGAGCAGAA AGCCTTGAAG GTGCTGAGGA TTTGTGAGCA GCGGCAGATG 
ACTGAGCAAG TTAAAAGCAT CTGTAAGATC TTGGCCATGA AGGCTGTTCG TAATAACCGC 
TTGGGCTCAG CACTCTCCTG GAGCATCCGT GCCAAAGATG CTGCCTTTGC CACACTCGTA 
TCTGACAGAT TCCTCCGGGA TTACTGTGAG AGAGGCTGCT TTTCTGACTT GGATCTCATT 
GACAATCTGG GGTCAGCCAT GATGCTCAGT GATCGACTGA CGTTTCTGGG AAAGTACCGG 
GAGTTCCACA GACTGTACGG GGAGAAGCGC TTTGGTGACG CTGCTTCTCT TCTGCTGTCC 
CTCATGACCT CTCAGATTGC ACCTCGTTCT TTCTGGATGA CTCTGCTCAC AGATGCCCTT 
CCTCTTTTGG AACAGAAACA GGTGATTTTT TCAGCAGAGC AGACATATGA GCTGATGCGG 
TGCCTGGAAG ACTTGGCCTC AGGGAGGCCA GAGTGTGGTG AACCTGATGC CCAGCGACTG 
CAGGATGACG ACATAGAGAC CACCAAGGTG GAGATGCTGA GACTGGCTCT TGCCCGGAAT 
CTTGCTCGGG CAATTATAAG GGAAGGCTCA CTGGAGGGTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nup85 ( XM_006533670.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533733.1 Mus musculus nucleoporin 85 (Nup85), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu46560
Clone ID Related Accession (Same CDS sequence)	XM_006533670.3 , XM_006533670.4
Accession Version	XM_006533670.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	nuclear pore complex protein Nup85 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533670.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TCGACTGCGA GCCCGCAGTA ACTTGGATTC CAGGTGTGAA TTCCAAGAAG 
AAGCAAATGT GTTTTGACTG GGGCCCCGGC GAGATGCTTC TGTGTGAAAC TTCCTTCAAC 
CAAACAGGCA AATCAGAGAA GGTGCCGAGC TGCCCTTTTA TCTACATCAT ACGGAAAGAT 
GTGGATGTTT ACTCTCAAAT TTTGAGAAAA CTCTTCAATG AATCCCATGG AATCTTTGTT 
GGCCTGCAAA AAATTGAGGA AGAACTGTCG GGGAAGTCCA GGAAAGCTCA ATTGGTTCGA 
GTGAGTAAAA ATTACCGTTC AGTCATACGG GCCTGTATGG AAGAAATGCA TCAGGTTGCA 
ATTGCTGCTA AAGATCCAGC CAGTGGCCGG CAGTTCAGCA GCCAGGTCTC CATTTTGTCA 
GCCATGGAGC TCATTTGGAA CCTGTGTGAG ATTCTCTTTA TTGAAGTAGC ACCAGCTGGC 
CCTCTCCTCC TTCACCTTCT TGACTGGGTC CGACTGCACG TGTGCGAGGT GGACAGTTTG 
TCGGCAGATG TCCTGGGCGG TGACAACCCA AGCAAGCATG AAAACTTCTG GGACCTGGTG 
ACTGTTCTGG TGCTTCAGGG CCGGCTCGAT GAGGCACGGC AGATGCTAGC CAAAGAAGCT 
GATGCCAACC CCTCTTGTGC AGGCATGTGC CGCGTCCTTG GGGACCTGAT GAGGACAATG 
CCCATTCTCA GCCCTGGCAA TACTCAGACA CTGACAGAGT TGGAGCTGAA GTGGCAGCAC 
TGGCGTGAGG AGTGTGAAAG ACACTTACAA GACAACACAT TTGCAGCCAA CCCCCGTCTG 
GAGTCTCTCT GCAAGATCAT GCTGGGAGAT GAGGCCGCCT TGTTGGAGCA GAAGGAGCTT 
CTGAGCAACT GGTACCATTT CTTAGTGACG AGGCTGCTGT ACTCTAACCC CACAGTGAAG 
CCCATTGACC TGCACTTCTA TGCCCAGTCC AGCCTAGACA TGTTTCTTGG AGGTGAGAGC 
AGTCCAGAAC CACTGGACAA CATCTTGATG GCGGCCTTTG AGTTCGACAT TCACCAGGTG 
ATCAAAGAGT GCAGCATCGC CCTGAGCAAC TGGTGGTTCG TAGCTCACCT CACAGACCTT 
TTGGATCACT GCAGACTCCT CCAGTCACAC AATCTCTATT TTGGTTCTAA CATGAGAGAA 
TTCCTCCTGC TGGAGTACGC CTCAGGACTG TTTGCTCACC ACAGCCTGTG GCAGCTGGGG 
GTGGACTACT TTGATTACTG CCCGGAGCTA GGCCGAGTTT CCTTGGAGCT GCACATTGAG 
CGGATTCCTC TCAACACAGA GCAGAAAGCC TTGAAGGTGC TGAGGATTTG TGAGCAGCGG 
CAGATGACTG AGCAAGTTAA AAGCATCTGT AAGATCTTGG CCATGAAGGC TGTTCGTAAT 
AACCGCTTGG GCTCAGCACT CTCCTGGAGC ATCCGTGCCA AAGATGCTGC CTTTGCCACA 
CTCGTATCTG ACAGATTCCT CCGGGATTAC TGTGAGAGAG GCTGCTTTTC TGACTTGGAT 
CTCATTGACA ATCTGGGGTC AGCCATGATG CTCAGTGATC GACTGACGTT TCTGGCTGGC 
TTCTGCAGCC ATACAGAAGC TCATTTACGC CTAAGCAGTA CCGTGTACAC GTGTCCAAGG 
CTCGAGCACC AATCCACTAT TCATTTCAGA ACAGCTGGAA GATGCCTCAC TAGAGGGATG 
GATGTGATTT TTTCAGCAGA GCAGACATAT GAGCTGATGC GGTGCCTGGA AGACTTGGCC 
TCAGGGAGGC CAGAGTGTGG TGAACCTGAT GCCCAGCGAC TGCAGGATGA CGACATAGAG 
ACCACCAAGG TGGAGATGCT GAGACTGGCT CTTGCCCGGA ATCTTGCTCG GGCAATTATA 
AGGGAAGGCT CACTGGAGGG TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533733.1
CDS	95..2041
Translation	MEELDCEPAVTWIPGVNSKKKQMCFDWGPGEMLLCETSFNQTGKSEKVPSCPFIYIIRKDVDVYSQILRKLFNESHGIFVGLQKIEEELSGKSRKAQLVRVSKNYRSVIRACMEEMHQVAIAAKDPASGRQFSSQVSILSAMELIWNLCEILFIEVAPAGPLLLHLLDWVRLHVCEVDSLSADVLGGDNPSKHENFWDLVTVLVLQGRLDEARQMLAKEADANPSCAGMCRVLGDLMRTMPILSPGNTQTLTELELKWQHWREECERHLQDNTFAANPRLESLCKIMLGDEAALLEQKELLSNWYHFLVTRLLYSNPTVKPIDLHFYAQSSLDMFLGGESSPEPLDNILMAAFEFDIHQVIKECSIALSNWWFVAHLTDLLDHCRLLQSHNLYFGSNMREFLLLEYASGLFAHHSLWQLGVDYFDYCPELGRVSLELHIERIPLNTEQKALKVLRICEQRQMTEQVKSICKILAMKAVRNNRLGSALSWSIRAKDAAFATLVSDRFLRDYCERGCFSDLDLIDNLGSAMMLSDRLTFLAGFCSHTEAHLRLSSTVYTCPRLEHQSTIHFRTAGRCLTRGMDVIFSAEQTYELMRCLEDLASGRPECGEPDAQRLQDDDIETTKVEMLRLALARNLARAIIREGSLEGS

Target ORF information:

RefSeq Version	XM_006533670.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nucleoporin 85 (Nup85), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533670.3

ORF Insert Sequence:

ATGGAGGAGC TCGACTGCGA GCCCGCAGTA ACTTGGATTC CAGGTGTGAA TTCCAAGAAG 
AAGCAAATGT GTTTTGACTG GGGCCCCGGC GAGATGCTTC TGTGTGAAAC TTCCTTCAAC 
CAAACAGGCA AATCAGAGAA GGTGCCGAGC TGCCCTTTTA TCTACATCAT ACGGAAAGAT 
GTGGATGTTT ACTCTCAAAT TTTGAGAAAA CTCTTCAATG AATCCCATGG AATCTTTGTT 
GGCCTGCAAA AAATTGAGGA AGAACTGTCG GGGAAGTCCA GGAAAGCTCA ATTGGTTCGA 
GTGAGTAAAA ATTACCGTTC AGTCATACGG GCCTGTATGG AAGAAATGCA TCAGGTTGCA 
ATTGCTGCTA AAGATCCAGC CAGTGGCCGG CAGTTCAGCA GCCAGGTCTC CATTTTGTCA 
GCCATGGAGC TCATTTGGAA CCTGTGTGAG ATTCTCTTTA TTGAAGTAGC ACCAGCTGGC 
CCTCTCCTCC TTCACCTTCT TGACTGGGTC CGACTGCACG TGTGCGAGGT GGACAGTTTG 
TCGGCAGATG TCCTGGGCGG TGACAACCCA AGCAAGCATG AAAACTTCTG GGACCTGGTG 
ACTGTTCTGG TGCTTCAGGG CCGGCTCGAT GAGGCACGGC AGATGCTAGC CAAAGAAGCT 
GATGCCAACC CCTCTTGTGC AGGCATGTGC CGCGTCCTTG GGGACCTGAT GAGGACAATG 
CCCATTCTCA GCCCTGGCAA TACTCAGACA CTGACAGAGT TGGAGCTGAA GTGGCAGCAC 
TGGCGTGAGG AGTGTGAAAG ACACTTACAA GACAACACAT TTGCAGCCAA CCCCCGTCTG 
GAGTCTCTCT GCAAGATCAT GCTGGGAGAT GAGGCCGCCT TGTTGGAGCA GAAGGAGCTT 
CTGAGCAACT GGTACCATTT CTTAGTGACG AGGCTGCTGT ACTCTAACCC CACAGTGAAG 
CCCATTGACC TGCACTTCTA TGCCCAGTCC AGCCTAGACA TGTTTCTTGG AGGTGAGAGC 
AGTCCAGAAC CACTGGACAA CATCTTGATG GCGGCCTTTG AGTTCGACAT TCACCAGGTG 
ATCAAAGAGT GCAGCATCGC CCTGAGCAAC TGGTGGTTCG TAGCTCACCT CACAGACCTT 
TTGGATCACT GCAGACTCCT CCAGTCACAC AATCTCTATT TTGGTTCTAA CATGAGAGAA 
TTCCTCCTGC TGGAGTACGC CTCAGGACTG TTTGCTCACC ACAGCCTGTG GCAGCTGGGG 
GTGGACTACT TTGATTACTG CCCGGAGCTA GGCCGAGTTT CCTTGGAGCT GCACATTGAG 
CGGATTCCTC TCAACACAGA GCAGAAAGCC TTGAAGGTGC TGAGGATTTG TGAGCAGCGG 
CAGATGACTG AGCAAGTTAA AAGCATCTGT AAGATCTTGG CCATGAAGGC TGTTCGTAAT 
AACCGCTTGG GCTCAGCACT CTCCTGGAGC ATCCGTGCCA AAGATGCTGC CTTTGCCACA 
CTCGTATCTG ACAGATTCCT CCGGGATTAC TGTGAGAGAG GCTGCTTTTC TGACTTGGAT 
CTCATTGACA ATCTGGGGTC AGCCATGATG CTCAGTGATC GACTGACGTT TCTGGCTGGC 
TTCTGCAGCC ATACAGAAGC TCATTTACGC CTAAGCAGTA CCGTGTACAC GTGTCCAAGG 
CTCGAGCACC AATCCACTAT TCATTTCAGA ACAGCTGGAA GATGCCTCAC TAGAGGGATG 
GATGTGATTT TTTCAGCAGA GCAGACATAT GAGCTGATGC GGTGCCTGGA AGACTTGGCC 
TCAGGGAGGC CAGAGTGTGG TGAACCTGAT GCCCAGCGAC TGCAGGATGA CGACATAGAG 
ACCACCAAGG TGGAGATGCT GAGACTGGCT CTTGCCCGGA ATCTTGCTCG GGCAATTATA 
AGGGAAGGCT CACTGGAGGG TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nup85 ( NM_001002929.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001002929.3 Mus musculus nucleoporin 85 (Nup85), mRNA.

Price & Availability↑

CloneID	OMu15655
Clone ID Related Accession (Same CDS sequence)	NM_001002929.4
Accession Version	NM_001002929.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	nuclear pore complex protein Nup85
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY032258.1, BC079856.1 and CF738553.1. On Jan 27, 2011 this sequence version replaced NM_001002929.3. ##Evidence-Data-START## Transcript exon combination :: AK017632.1, BC079856.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGC TCGACTGCGA GCCCGCAGTA ACTTGGATTC CAGGTGTGAA TTCCAAGAAG 
AAGCAAATGT GTTTTGACTG GGGCCCCGGC GAGATGCTTC TGTGTGAAAC TTCCTTCAAC 
CAAACAGGCA AATCAGAGAA GGTGCCGAGC TGCCCTTTTA TCTACATCAT ACGGAAAGAT 
GTGGATGTTT ACTCTCAAAT TTTGAGAAAA CTCTTCAATG AATCCCATGG AATCTTTGTT 
GGCCTGCAAA AAATTGAGGA AGAACTGTCG GGGAAGTCCA GGAAAGCTCA ATTGGTTCGA 
GTGAGTAAAA ATTACCGTTC AGTCATACGG GCCTGTATGG AAGAAATGCA TCAGGTTGCA 
ATTGCTGCTA AAGATCCAGC CAGTGGCCGG CAGTTCAGCA GCCAGGTCTC CATTTTGTCA 
GCCATGGAGC TCATTTGGAA CCTGTGTGAG ATTCTCTTTA TTGAAGTAGC ACCAGCTGGC 
CCTCTCCTCC TTCACCTTCT TGACTGGGTC CGACTGCACG TGTGCGAGGT GGACAGTTTG 
TCGGCAGATG TCCTGGGCGG TGACAACCCA AGCAAGCATG AAAACTTCTG GGACCTGGTG 
ACTGTTCTGG TGCTTCAGGG CCGGCTCGAT GAGGCACGGC AGATGCTAGC CAAAGAAGCT 
GATGCCAACC CCTCTTGTGC AGGCATGTGC CGCGTCCTTG GGGACCTGAT GAGGACAATG 
CCCATTCTCA GCCCTGGCAA TACTCAGACA CTGACAGAGT TGGAGCTGAA GTGGCAGCAC 
TGGCGTGAGG AGTGTGAAAG ACACTTACAA GACAACACAT TTGCAGCCAA CCCCCGTCTG 
GAGTCTCTCT GCAAGATCAT GCTGGGAGAT GAGGCCGCCT TGTTGGAGCA GAAGGAGCTT 
CTGAGCAACT GGTACCATTT CTTAGTGACG AGGCTGCTGT ACTCTAACCC CACAGTGAAG 
CCCATTGACC TGCACTTCTA TGCCCAGTCC AGCCTAGACA TGTTTCTTGG AGGTGAGAGC 
AGTCCAGAAC CACTGGACAA CATCTTGATG GCGGCCTTTG AGTTCGACAT TCACCAGGTG 
ATCAAAGAGT GCAGCATCGC CCTGAGCAAC TGGTGGTTCG TAGCTCACCT CACAGACCTT 
TTGGATCACT GCAGACTCCT CCAGTCACAC AATCTCTATT TTGGTTCTAA CATGAGAGAA 
TTCCTCCTGC TGGAGTACGC CTCAGGACTG TTTGCTCACC ACAGCCTGTG GCAGCTGGGG 
GTGGACTACT TTGATTACTG CCCGGAGCTA GGCCGAGTTT CCTTGGAGCT GCACATTGAG 
CGGATTCCTC TCAACACAGA GCAGAAAGCC TTGAAGGTGC TGAGGATTTG TGAGCAGCGG 
CAGATGACTG AGCAAGTTAA AAGCATCTGT AAGATCTTGG CCATGAAGGC TGTTCGTAAT 
AACCGCTTGG GCTCAGCACT CTCCTGGAGC ATCCGTGCCA AAGATGCTGC CTTTGCCACA 
CTCGTATCTG ACAGATTCCT CCGGGATTAC TGTGAGAGAG GCTGCTTTTC TGACTTGGAT 
CTCATTGACA ATCTGGGGTC AGCCATGATG CTCAGTGATC GACTGACGTT TCTGGGAAAG 
TACCGGGAGT TCCACAGACT GTACGGGGAG AAGCGCTTTG GTGACGCTGC TTCTCTTCTG 
CTGTCCCTCA TGACCTCTCA GATTGCACCT CGTTCTTTCT GGATGACTCT GCTCACAGAT 
GCCCTTCCTC TTTTGGAACA GAAACAGGTG ATTTTTTCAG CAGAGCAGAC ATATGAGCTG 
ATGCGGTGCC TGGAAGACTT GGCCTCAGGG AGGCCAGAGT GTGGTGAACC TGATGCCCAG 
CGACTGCAGG ATGACGACAT AGAGACCACC AAGGTGGAGA TGCTGAGACT GGCTCTTGCC 
CGGAATCTTG CTCGGGCAAT TATAAGGGAA GGCTCACTGG AGGGTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002929.3
CDS	87..2057
Translation	MEELDCEPAVTWIPGVNSKKKQMCFDWGPGEMLLCETSFNQTGKSEKVPSCPFIYIIRKDVDVYSQILRKLFNESHGIFVGLQKIEEELSGKSRKAQLVRVSKNYRSVIRACMEEMHQVAIAAKDPASGRQFSSQVSILSAMELIWNLCEILFIEVAPAGPLLLHLLDWVRLHVCEVDSLSADVLGGDNPSKHENFWDLVTVLVLQGRLDEARQMLAKEADANPSCAGMCRVLGDLMRTMPILSPGNTQTLTELELKWQHWREECERHLQDNTFAANPRLESLCKIMLGDEAALLEQKELLSNWYHFLVTRLLYSNPTVKPIDLHFYAQSSLDMFLGGESSPEPLDNILMAAFEFDIHQVIKECSIALSNWWFVAHLTDLLDHCRLLQSHNLYFGSNMREFLLLEYASGLFAHHSLWQLGVDYFDYCPELGRVSLELHIERIPLNTEQKALKVLRICEQRQMTEQVKSICKILAMKAVRNNRLGSALSWSIRAKDAAFATLVSDRFLRDYCERGCFSDLDLIDNLGSAMMLSDRLTFLGKYREFHRLYGEKRFGDAASLLLSLMTSQIAPRSFWMTLLTDALPLLEQKQVIFSAEQTYELMRCLEDLASGRPECGEPDAQRLQDDDIETTKVEMLRLALARNLARAIIREGSLEGS

Target ORF information:

RefSeq Version	NM_001002929.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus nucleoporin 85 (Nup85), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002929.4

ORF Insert Sequence:

ATGGAGGAGC TCGACTGCGA GCCCGCAGTA ACTTGGATTC CAGGTGTGAA TTCCAAGAAG 
AAGCAAATGT GTTTTGACTG GGGCCCCGGC GAGATGCTTC TGTGTGAAAC TTCCTTCAAC 
CAAACAGGCA AATCAGAGAA GGTGCCGAGC TGCCCTTTTA TCTACATCAT ACGGAAAGAT 
GTGGATGTTT ACTCTCAAAT TTTGAGAAAA CTCTTCAATG AATCCCATGG AATCTTTGTT 
GGCCTGCAAA AAATTGAGGA AGAACTGTCG GGGAAGTCCA GGAAAGCTCA ATTGGTTCGA 
GTGAGTAAAA ATTACCGTTC AGTCATACGG GCCTGTATGG AAGAAATGCA TCAGGTTGCA 
ATTGCTGCTA AAGATCCAGC CAGTGGCCGG CAGTTCAGCA GCCAGGTCTC CATTTTGTCA 
GCCATGGAGC TCATTTGGAA CCTGTGTGAG ATTCTCTTTA TTGAAGTAGC ACCAGCTGGC 
CCTCTCCTCC TTCACCTTCT TGACTGGGTC CGACTGCACG TGTGCGAGGT GGACAGTTTG 
TCGGCAGATG TCCTGGGCGG TGACAACCCA AGCAAGCATG AAAACTTCTG GGACCTGGTG 
ACTGTTCTGG TGCTTCAGGG CCGGCTCGAT GAGGCACGGC AGATGCTAGC CAAAGAAGCT 
GATGCCAACC CCTCTTGTGC AGGCATGTGC CGCGTCCTTG GGGACCTGAT GAGGACAATG 
CCCATTCTCA GCCCTGGCAA TACTCAGACA CTGACAGAGT TGGAGCTGAA GTGGCAGCAC 
TGGCGTGAGG AGTGTGAAAG ACACTTACAA GACAACACAT TTGCAGCCAA CCCCCGTCTG 
GAGTCTCTCT GCAAGATCAT GCTGGGAGAT GAGGCCGCCT TGTTGGAGCA GAAGGAGCTT 
CTGAGCAACT GGTACCATTT CTTAGTGACG AGGCTGCTGT ACTCTAACCC CACAGTGAAG 
CCCATTGACC TGCACTTCTA TGCCCAGTCC AGCCTAGACA TGTTTCTTGG AGGTGAGAGC 
AGTCCAGAAC CACTGGACAA CATCTTGATG GCGGCCTTTG AGTTCGACAT TCACCAGGTG 
ATCAAAGAGT GCAGCATCGC CCTGAGCAAC TGGTGGTTCG TAGCTCACCT CACAGACCTT 
TTGGATCACT GCAGACTCCT CCAGTCACAC AATCTCTATT TTGGTTCTAA CATGAGAGAA 
TTCCTCCTGC TGGAGTACGC CTCAGGACTG TTTGCTCACC ACAGCCTGTG GCAGCTGGGG 
GTGGACTACT TTGATTACTG CCCGGAGCTA GGCCGAGTTT CCTTGGAGCT GCACATTGAG 
CGGATTCCTC TCAACACAGA GCAGAAAGCC TTGAAGGTGC TGAGGATTTG TGAGCAGCGG 
CAGATGACTG AGCAAGTTAA AAGCATCTGT AAGATCTTGG CCATGAAGGC TGTTCGTAAT 
AACCGCTTGG GCTCAGCACT CTCCTGGAGC ATCCGTGCCA AAGATGCTGC CTTTGCCACA 
CTCGTATCTG ACAGATTCCT CCGGGATTAC TGTGAGAGAG GCTGCTTTTC TGACTTGGAT 
CTCATTGACA ATCTGGGGTC AGCCATGATG CTCAGTGATC GACTGACGTT TCTGGGAAAG 
TACCGGGAGT TCCACAGACT GTACGGGGAG AAGCGCTTTG GTGACGCTGC TTCTCTTCTG 
CTGTCCCTCA TGACCTCTCA GATTGCACCT CGTTCTTTCT GGATGACTCT GCTCACAGAT 
GCCCTTCCTC TTTTGGAACA GAAACAGGTG ATTTTTTCAG CAGAGCAGAC ATATGAGCTG 
ATGCGGTGCC TGGAAGACTT GGCCTCAGGG AGGCCAGAGT GTGGTGAACC TGATGCCCAG 
CGACTGCAGG ATGACGACAT AGAGACCACC AAGGTGGAGA TGCTGAGACT GGCTCTTGCC 
CGGAATCTTG CTCGGGCAAT TATAAGGGAA GGCTCACTGG AGGGTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nup85 ( XM_006533670.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533733.1 Mus musculus nucleoporin 85 (Nup85), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu46560
Clone ID Related Accession (Same CDS sequence)	XM_006533670.3 , XM_006533670.4
Accession Version	XM_006533670.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nuclear pore complex protein Nup85 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533670.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TCGACTGCGA GCCCGCAGTA ACTTGGATTC CAGGTGTGAA TTCCAAGAAG 
AAGCAAATGT GTTTTGACTG GGGCCCCGGC GAGATGCTTC TGTGTGAAAC TTCCTTCAAC 
CAAACAGGCA AATCAGAGAA GGTGCCGAGC TGCCCTTTTA TCTACATCAT ACGGAAAGAT 
GTGGATGTTT ACTCTCAAAT TTTGAGAAAA CTCTTCAATG AATCCCATGG AATCTTTGTT 
GGCCTGCAAA AAATTGAGGA AGAACTGTCG GGGAAGTCCA GGAAAGCTCA ATTGGTTCGA 
GTGAGTAAAA ATTACCGTTC AGTCATACGG GCCTGTATGG AAGAAATGCA TCAGGTTGCA 
ATTGCTGCTA AAGATCCAGC CAGTGGCCGG CAGTTCAGCA GCCAGGTCTC CATTTTGTCA 
GCCATGGAGC TCATTTGGAA CCTGTGTGAG ATTCTCTTTA TTGAAGTAGC ACCAGCTGGC 
CCTCTCCTCC TTCACCTTCT TGACTGGGTC CGACTGCACG TGTGCGAGGT GGACAGTTTG 
TCGGCAGATG TCCTGGGCGG TGACAACCCA AGCAAGCATG AAAACTTCTG GGACCTGGTG 
ACTGTTCTGG TGCTTCAGGG CCGGCTCGAT GAGGCACGGC AGATGCTAGC CAAAGAAGCT 
GATGCCAACC CCTCTTGTGC AGGCATGTGC CGCGTCCTTG GGGACCTGAT GAGGACAATG 
CCCATTCTCA GCCCTGGCAA TACTCAGACA CTGACAGAGT TGGAGCTGAA GTGGCAGCAC 
TGGCGTGAGG AGTGTGAAAG ACACTTACAA GACAACACAT TTGCAGCCAA CCCCCGTCTG 
GAGTCTCTCT GCAAGATCAT GCTGGGAGAT GAGGCCGCCT TGTTGGAGCA GAAGGAGCTT 
CTGAGCAACT GGTACCATTT CTTAGTGACG AGGCTGCTGT ACTCTAACCC CACAGTGAAG 
CCCATTGACC TGCACTTCTA TGCCCAGTCC AGCCTAGACA TGTTTCTTGG AGGTGAGAGC 
AGTCCAGAAC CACTGGACAA CATCTTGATG GCGGCCTTTG AGTTCGACAT TCACCAGGTG 
ATCAAAGAGT GCAGCATCGC CCTGAGCAAC TGGTGGTTCG TAGCTCACCT CACAGACCTT 
TTGGATCACT GCAGACTCCT CCAGTCACAC AATCTCTATT TTGGTTCTAA CATGAGAGAA 
TTCCTCCTGC TGGAGTACGC CTCAGGACTG TTTGCTCACC ACAGCCTGTG GCAGCTGGGG 
GTGGACTACT TTGATTACTG CCCGGAGCTA GGCCGAGTTT CCTTGGAGCT GCACATTGAG 
CGGATTCCTC TCAACACAGA GCAGAAAGCC TTGAAGGTGC TGAGGATTTG TGAGCAGCGG 
CAGATGACTG AGCAAGTTAA AAGCATCTGT AAGATCTTGG CCATGAAGGC TGTTCGTAAT 
AACCGCTTGG GCTCAGCACT CTCCTGGAGC ATCCGTGCCA AAGATGCTGC CTTTGCCACA 
CTCGTATCTG ACAGATTCCT CCGGGATTAC TGTGAGAGAG GCTGCTTTTC TGACTTGGAT 
CTCATTGACA ATCTGGGGTC AGCCATGATG CTCAGTGATC GACTGACGTT TCTGGCTGGC 
TTCTGCAGCC ATACAGAAGC TCATTTACGC CTAAGCAGTA CCGTGTACAC GTGTCCAAGG 
CTCGAGCACC AATCCACTAT TCATTTCAGA ACAGCTGGAA GATGCCTCAC TAGAGGGATG 
GATGTGATTT TTTCAGCAGA GCAGACATAT GAGCTGATGC GGTGCCTGGA AGACTTGGCC 
TCAGGGAGGC CAGAGTGTGG TGAACCTGAT GCCCAGCGAC TGCAGGATGA CGACATAGAG 
ACCACCAAGG TGGAGATGCT GAGACTGGCT CTTGCCCGGA ATCTTGCTCG GGCAATTATA 
AGGGAAGGCT CACTGGAGGG TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533733.1
CDS	99..2045
Translation	MEELDCEPAVTWIPGVNSKKKQMCFDWGPGEMLLCETSFNQTGKSEKVPSCPFIYIIRKDVDVYSQILRKLFNESHGIFVGLQKIEEELSGKSRKAQLVRVSKNYRSVIRACMEEMHQVAIAAKDPASGRQFSSQVSILSAMELIWNLCEILFIEVAPAGPLLLHLLDWVRLHVCEVDSLSADVLGGDNPSKHENFWDLVTVLVLQGRLDEARQMLAKEADANPSCAGMCRVLGDLMRTMPILSPGNTQTLTELELKWQHWREECERHLQDNTFAANPRLESLCKIMLGDEAALLEQKELLSNWYHFLVTRLLYSNPTVKPIDLHFYAQSSLDMFLGGESSPEPLDNILMAAFEFDIHQVIKECSIALSNWWFVAHLTDLLDHCRLLQSHNLYFGSNMREFLLLEYASGLFAHHSLWQLGVDYFDYCPELGRVSLELHIERIPLNTEQKALKVLRICEQRQMTEQVKSICKILAMKAVRNNRLGSALSWSIRAKDAAFATLVSDRFLRDYCERGCFSDLDLIDNLGSAMMLSDRLTFLAGFCSHTEAHLRLSSTVYTCPRLEHQSTIHFRTAGRCLTRGMDVIFSAEQTYELMRCLEDLASGRPECGEPDAQRLQDDDIETTKVEMLRLALARNLARAIIREGSLEGS

Target ORF information:

RefSeq Version	XM_006533670.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus nucleoporin 85 (Nup85), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533670.4

ORF Insert Sequence:

ATGGAGGAGC TCGACTGCGA GCCCGCAGTA ACTTGGATTC CAGGTGTGAA TTCCAAGAAG 
AAGCAAATGT GTTTTGACTG GGGCCCCGGC GAGATGCTTC TGTGTGAAAC TTCCTTCAAC 
CAAACAGGCA AATCAGAGAA GGTGCCGAGC TGCCCTTTTA TCTACATCAT ACGGAAAGAT 
GTGGATGTTT ACTCTCAAAT TTTGAGAAAA CTCTTCAATG AATCCCATGG AATCTTTGTT 
GGCCTGCAAA AAATTGAGGA AGAACTGTCG GGGAAGTCCA GGAAAGCTCA ATTGGTTCGA 
GTGAGTAAAA ATTACCGTTC AGTCATACGG GCCTGTATGG AAGAAATGCA TCAGGTTGCA 
ATTGCTGCTA AAGATCCAGC CAGTGGCCGG CAGTTCAGCA GCCAGGTCTC CATTTTGTCA 
GCCATGGAGC TCATTTGGAA CCTGTGTGAG ATTCTCTTTA TTGAAGTAGC ACCAGCTGGC 
CCTCTCCTCC TTCACCTTCT TGACTGGGTC CGACTGCACG TGTGCGAGGT GGACAGTTTG 
TCGGCAGATG TCCTGGGCGG TGACAACCCA AGCAAGCATG AAAACTTCTG GGACCTGGTG 
ACTGTTCTGG TGCTTCAGGG CCGGCTCGAT GAGGCACGGC AGATGCTAGC CAAAGAAGCT 
GATGCCAACC CCTCTTGTGC AGGCATGTGC CGCGTCCTTG GGGACCTGAT GAGGACAATG 
CCCATTCTCA GCCCTGGCAA TACTCAGACA CTGACAGAGT TGGAGCTGAA GTGGCAGCAC 
TGGCGTGAGG AGTGTGAAAG ACACTTACAA GACAACACAT TTGCAGCCAA CCCCCGTCTG 
GAGTCTCTCT GCAAGATCAT GCTGGGAGAT GAGGCCGCCT TGTTGGAGCA GAAGGAGCTT 
CTGAGCAACT GGTACCATTT CTTAGTGACG AGGCTGCTGT ACTCTAACCC CACAGTGAAG 
CCCATTGACC TGCACTTCTA TGCCCAGTCC AGCCTAGACA TGTTTCTTGG AGGTGAGAGC 
AGTCCAGAAC CACTGGACAA CATCTTGATG GCGGCCTTTG AGTTCGACAT TCACCAGGTG 
ATCAAAGAGT GCAGCATCGC CCTGAGCAAC TGGTGGTTCG TAGCTCACCT CACAGACCTT 
TTGGATCACT GCAGACTCCT CCAGTCACAC AATCTCTATT TTGGTTCTAA CATGAGAGAA 
TTCCTCCTGC TGGAGTACGC CTCAGGACTG TTTGCTCACC ACAGCCTGTG GCAGCTGGGG 
GTGGACTACT TTGATTACTG CCCGGAGCTA GGCCGAGTTT CCTTGGAGCT GCACATTGAG 
CGGATTCCTC TCAACACAGA GCAGAAAGCC TTGAAGGTGC TGAGGATTTG TGAGCAGCGG 
CAGATGACTG AGCAAGTTAA AAGCATCTGT AAGATCTTGG CCATGAAGGC TGTTCGTAAT 
AACCGCTTGG GCTCAGCACT CTCCTGGAGC ATCCGTGCCA AAGATGCTGC CTTTGCCACA 
CTCGTATCTG ACAGATTCCT CCGGGATTAC TGTGAGAGAG GCTGCTTTTC TGACTTGGAT 
CTCATTGACA ATCTGGGGTC AGCCATGATG CTCAGTGATC GACTGACGTT TCTGGCTGGC 
TTCTGCAGCC ATACAGAAGC TCATTTACGC CTAAGCAGTA CCGTGTACAC GTGTCCAAGG 
CTCGAGCACC AATCCACTAT TCATTTCAGA ACAGCTGGAA GATGCCTCAC TAGAGGGATG 
GATGTGATTT TTTCAGCAGA GCAGACATAT GAGCTGATGC GGTGCCTGGA AGACTTGGCC 
TCAGGGAGGC CAGAGTGTGG TGAACCTGAT GCCCAGCGAC TGCAGGATGA CGACATAGAG 
ACCACCAAGG TGGAGATGCT GAGACTGGCT CTTGCCCGGA ATCTTGCTCG GGCAATTATA 
AGGGAAGGCT CACTGGAGGG TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nup85 ( XM_006533671.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533734.1 Mus musculus nucleoporin 85 (Nup85), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu46561
Clone ID Related Accession (Same CDS sequence)	XM_006533671.2 , XM_006533671.3
Accession Version	XM_006533671.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nuclear pore complex protein Nup85 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533671.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTTTTG ACTGGGGCCC CGGCGAGATG CTTCTGTGTG AAACTTCCTT CAACCAAACA 
GGCAAATCAG AGAAGGTGCC GAGCTGCCCT TTTATCTACA TCATACGGAA AGATGTGGAT 
GTTTACTCTC AAATTTTGAG AAAACTCTTC AATGAATCCC ATGGAATCTT TGTTGGCCTG 
CAAAAAATTG AGGAAGAACT GTCGGGGAAG TCCAGGAAAG CTCAATTGGT TCGAGTGAGT 
AAAAATTACC GTTCAGTCAT ACGGGCCTGT ATGGAAGAAA TGCATCAGGT TGCAATTGCT 
GCTAAAGATC CAGCCAGTGG CCGGCAGTTC AGCAGCCAGG TCTCCATTTT GTCAGCCATG 
GAGCTCATTT GGAACCTGTG TGAGATTCTC TTTATTGAAG TAGCACCAGC TGGCCCTCTC 
CTCCTTCACC TTCTTGACTG GGTCCGACTG CACGTGTGCG AGGTGGACAG TTTGTCGGCA 
GATGTCCTGG GCGGTGACAA CCCAAGCAAG CATGAAAACT TCTGGGACCT GGTGACTGTT 
CTGGTGCTTC AGGGCCGGCT CGATGAGGCA CGGCAGATGC TAGCCAAAGA AGCTGATGCC 
AACCCCTCTT GTGCAGGCAT GTGCCGCGTC CTTGGGGACC TGATGAGGAC AATGCCCATT 
CTCAGCCCTG GCAATACTCA GACACTGACA GAGTTGGAGC TGAAGTGGCA GCACTGGCGT 
GAGGAGTGTG AAAGACACTT ACAAGACAAC ACATTTGCAG CCAACCCCCG TCTGGAGTCT 
CTCTGCAAGA TCATGCTGGG AGATGAGGCC GCCTTGTTGG AGCAGAAGGA GCTTCTGAGC 
AACTGGTACC ATTTCTTAGT GACGAGGCTG CTGTACTCTA ACCCCACAGT GAAGCCCATT 
GACCTGCACT TCTATGCCCA GTCCAGCCTA GACATGTTTC TTGGAGGTGA GAGCAGTCCA 
GAACCACTGG ACAACATCTT GATGGCGGCC TTTGAGTTCG ACATTCACCA GGTGATCAAA 
GAGTGCAGCA TCGCCCTGAG CAACTGGTGG TTCGTAGCTC ACCTCACAGA CCTTTTGGAT 
CACTGCAGAC TCCTCCAGTC ACACAATCTC TATTTTGGTT CTAACATGAG AGAATTCCTC 
CTGCTGGAGT ACGCCTCAGG ACTGTTTGCT CACCACAGCC TGTGGCAGCT GGGGGTGGAC 
TACTTTGATT ACTGCCCGGA GCTAGGCCGA GTTTCCTTGG AGCTGCACAT TGAGCGGATT 
CCTCTCAACA CAGAGCAGAA AGCCTTGAAG GTGCTGAGGA TTTGTGAGCA GCGGCAGATG 
ACTGAGCAAG TTAAAAGCAT CTGTAAGATC TTGGCCATGA AGGCTGTTCG TAATAACCGC 
TTGGGCTCAG CACTCTCCTG GAGCATCCGT GCCAAAGATG CTGCCTTTGC CACACTCGTA 
TCTGACAGAT TCCTCCGGGA TTACTGTGAG AGAGGCTGCT TTTCTGACTT GGATCTCATT 
GACAATCTGG GGTCAGCCAT GATGCTCAGT GATCGACTGA CGTTTCTGGG AAAGTACCGG 
GAGTTCCACA GACTGTACGG GGAGAAGCGC TTTGGTGACG CTGCTTCTCT TCTGCTGTCC 
CTCATGACCT CTCAGATTGC ACCTCGTTCT TTCTGGATGA CTCTGCTCAC AGATGCCCTT 
CCTCTTTTGG AACAGAAACA GGTGATTTTT TCAGCAGAGC AGACATATGA GCTGATGCGG 
TGCCTGGAAG ACTTGGCCTC AGGGAGGCCA GAGTGTGGTG AACCTGATGC CCAGCGACTG 
CAGGATGACG ACATAGAGAC CACCAAGGTG GAGATGCTGA GACTGGCTCT TGCCCGGAAT 
CTTGCTCGGG CAATTATAAG GGAAGGCTCA CTGGAGGGTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533734.1
CDS	153..2057
Translation	MCFDWGPGEMLLCETSFNQTGKSEKVPSCPFIYIIRKDVDVYSQILRKLFNESHGIFVGLQKIEEELSGKSRKAQLVRVSKNYRSVIRACMEEMHQVAIAAKDPASGRQFSSQVSILSAMELIWNLCEILFIEVAPAGPLLLHLLDWVRLHVCEVDSLSADVLGGDNPSKHENFWDLVTVLVLQGRLDEARQMLAKEADANPSCAGMCRVLGDLMRTMPILSPGNTQTLTELELKWQHWREECERHLQDNTFAANPRLESLCKIMLGDEAALLEQKELLSNWYHFLVTRLLYSNPTVKPIDLHFYAQSSLDMFLGGESSPEPLDNILMAAFEFDIHQVIKECSIALSNWWFVAHLTDLLDHCRLLQSHNLYFGSNMREFLLLEYASGLFAHHSLWQLGVDYFDYCPELGRVSLELHIERIPLNTEQKALKVLRICEQRQMTEQVKSICKILAMKAVRNNRLGSALSWSIRAKDAAFATLVSDRFLRDYCERGCFSDLDLIDNLGSAMMLSDRLTFLGKYREFHRLYGEKRFGDAASLLLSLMTSQIAPRSFWMTLLTDALPLLEQKQVIFSAEQTYELMRCLEDLASGRPECGEPDAQRLQDDDIETTKVEMLRLALARNLARAIIREGSLEGS

Target ORF information:

RefSeq Version	XM_006533671.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nucleoporin 85 (Nup85), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533671.3

ORF Insert Sequence:

ATGTGTTTTG ACTGGGGCCC CGGCGAGATG CTTCTGTGTG AAACTTCCTT CAACCAAACA 
GGCAAATCAG AGAAGGTGCC GAGCTGCCCT TTTATCTACA TCATACGGAA AGATGTGGAT 
GTTTACTCTC AAATTTTGAG AAAACTCTTC AATGAATCCC ATGGAATCTT TGTTGGCCTG 
CAAAAAATTG AGGAAGAACT GTCGGGGAAG TCCAGGAAAG CTCAATTGGT TCGAGTGAGT 
AAAAATTACC GTTCAGTCAT ACGGGCCTGT ATGGAAGAAA TGCATCAGGT TGCAATTGCT 
GCTAAAGATC CAGCCAGTGG CCGGCAGTTC AGCAGCCAGG TCTCCATTTT GTCAGCCATG 
GAGCTCATTT GGAACCTGTG TGAGATTCTC TTTATTGAAG TAGCACCAGC TGGCCCTCTC 
CTCCTTCACC TTCTTGACTG GGTCCGACTG CACGTGTGCG AGGTGGACAG TTTGTCGGCA 
GATGTCCTGG GCGGTGACAA CCCAAGCAAG CATGAAAACT TCTGGGACCT GGTGACTGTT 
CTGGTGCTTC AGGGCCGGCT CGATGAGGCA CGGCAGATGC TAGCCAAAGA AGCTGATGCC 
AACCCCTCTT GTGCAGGCAT GTGCCGCGTC CTTGGGGACC TGATGAGGAC AATGCCCATT 
CTCAGCCCTG GCAATACTCA GACACTGACA GAGTTGGAGC TGAAGTGGCA GCACTGGCGT 
GAGGAGTGTG AAAGACACTT ACAAGACAAC ACATTTGCAG CCAACCCCCG TCTGGAGTCT 
CTCTGCAAGA TCATGCTGGG AGATGAGGCC GCCTTGTTGG AGCAGAAGGA GCTTCTGAGC 
AACTGGTACC ATTTCTTAGT GACGAGGCTG CTGTACTCTA ACCCCACAGT GAAGCCCATT 
GACCTGCACT TCTATGCCCA GTCCAGCCTA GACATGTTTC TTGGAGGTGA GAGCAGTCCA 
GAACCACTGG ACAACATCTT GATGGCGGCC TTTGAGTTCG ACATTCACCA GGTGATCAAA 
GAGTGCAGCA TCGCCCTGAG CAACTGGTGG TTCGTAGCTC ACCTCACAGA CCTTTTGGAT 
CACTGCAGAC TCCTCCAGTC ACACAATCTC TATTTTGGTT CTAACATGAG AGAATTCCTC 
CTGCTGGAGT ACGCCTCAGG ACTGTTTGCT CACCACAGCC TGTGGCAGCT GGGGGTGGAC 
TACTTTGATT ACTGCCCGGA GCTAGGCCGA GTTTCCTTGG AGCTGCACAT TGAGCGGATT 
CCTCTCAACA CAGAGCAGAA AGCCTTGAAG GTGCTGAGGA TTTGTGAGCA GCGGCAGATG 
ACTGAGCAAG TTAAAAGCAT CTGTAAGATC TTGGCCATGA AGGCTGTTCG TAATAACCGC 
TTGGGCTCAG CACTCTCCTG GAGCATCCGT GCCAAAGATG CTGCCTTTGC CACACTCGTA 
TCTGACAGAT TCCTCCGGGA TTACTGTGAG AGAGGCTGCT TTTCTGACTT GGATCTCATT 
GACAATCTGG GGTCAGCCAT GATGCTCAGT GATCGACTGA CGTTTCTGGG AAAGTACCGG 
GAGTTCCACA GACTGTACGG GGAGAAGCGC TTTGGTGACG CTGCTTCTCT TCTGCTGTCC 
CTCATGACCT CTCAGATTGC ACCTCGTTCT TTCTGGATGA CTCTGCTCAC AGATGCCCTT 
CCTCTTTTGG AACAGAAACA GGTGATTTTT TCAGCAGAGC AGACATATGA GCTGATGCGG 
TGCCTGGAAG ACTTGGCCTC AGGGAGGCCA GAGTGTGGTG AACCTGATGC CCAGCGACTG 
CAGGATGACG ACATAGAGAC CACCAAGGTG GAGATGCTGA GACTGGCTCT TGCCCGGAAT 
CTTGCTCGGG CAATTATAAG GGAAGGCTCA CTGGAGGGTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nup85 ( XM_030246116.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101976.1 Mus musculus nucleoporin 85 (Nup85), transcript variant X3, mRNA.

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CloneID	OMu85832
Clone ID Related Accession (Same CDS sequence)	XM_030246116.1
Accession Version	XM_030246116.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nuclear pore complex protein Nup85 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTTTTG ACTGGGGCCC CGGCGAGATG CTTCTGTGTG AAACTTCCTT CAACCAAACA 
GGCAAATCAG AGAAGGTGCC GAGCTGCCCT TTTATCTACA TCATACGGAA AGATGTGGAT 
GTTTACTCTC AAATTTTGAG AAAACTCTTC AATGAATCCC ATGGAATCTT TGTTGGCCTG 
CAAAAAATTG AGGAAGAACT GTCGGGGAAG TCCAGGAAAG CTCAATTGGT TCGAGTGAGT 
AAAAATTACC GTTCAGTCAT ACGGGCCTGT ATGGAAGAAA TGCATCAGGT TGCAATTGCT 
GCTAAAGATC CAGCCAGTGG CCGGCAGTTC AGCAGCCAGG TCTCCATTTT GTCAGCCATG 
GAGCTCATTT GGAACCTGTG TGAGATTCTC TTTATTGAAG TAGCACCAGC TGGCCCTCTC 
CTCCTTCACC TTCTTGACTG GGTCCGACTG CACGTGTGCG AGGTGGACAG TTTGTCGGCA 
GATGTCCTGG GCGGTGACAA CCCAAGCAAG CATGAAAACT TCTGGGACCT GGTGACTGTT 
CTGGTGCTTC AGGGCCGGCT CGATGAGGCA CGGCAGATGC TAGCCAAAGA AGCTGATGCC 
AACCCCTCTT GTGCAGGCAT GTGCCGCGTC CTTGGGGACC TGATGAGGAC AATGCCCATT 
CTCAGCCCTG GCAATACTCA GACACTGACA GAGTTGGAGC TGAAGTGGCA GCACTGGCGT 
GAGGAGTGTG AAAGACACTT ACAAGACAAC ACATTTGCAG CCAACCCCCG TCTGGAGTCT 
CTCTGCAAGA TCATGCTGGG AGATGAGGCC GCCTTGTTGG AGCAGAAGGA GCTTCTGAGC 
AACTGGTACC ATTTCTTAGT GACGAGGCTG CTGTACTCTA ACCCCACAGT GAAGCCCATT 
GACCTGCACT TCTATGCCCA GTCCAGCCTA GACATGTTTC TTGGAGGTGA GAGCAGTCCA 
GAACCACTGG ACAACATCTT GATGGCGGCC TTTGAGTTCG ACATTCACCA GGTGATCAAA 
GAGTGCAGCA TCGCCCTGAG CAACTGGTGG TTCGTAGCTC ACCTCACAGA CCTTTTGGAT 
CACTGCAGAC TCCTCCAGTC ACACAATCTC TATTTTGGTT CTAACATGAG AGAATTCCTC 
CTGCTGGAGT ACGCCTCAGG ACTGTTTGCT CACCACAGCC TGTGGCAGCT GGGGGTGGAC 
TACTTTGATT ACTGCCCGGA GCTAGGCCGA GTTTCCTTGG AGCTGCACAT TGAGCGGATT 
CCTCTCAACA CAGAGCAGAA AGCCTTGAAG GTGCTGAGGA TTTGTGAGCA GCGGCAGATG 
ACTGAGCAAG TTAAAAGCAT CTGTAAGATC TTGGCCATGA AGGCTGTTCG TAATAACCGC 
TTGGGCTCAG CACTCTCCTG GAGCATCCGT GCCAAAGATG CTGCCTTTGC CACACTCGTA 
TCTGACAGAT TCCTCCGGGA TTACTGTGAG AGAGGCTGCT TTTCTGACTT GGATCTCATT 
GACAATCTGG GGTCAGCCAT GATGCTCAGT GATCGACTGA CGTTTCTGGC TGGCTTCTGC 
AGCCATACAG AAGCTCATTT ACGCCTAAGC AGTACCGTGT ACACGTGTCC AAGGCTCGAG 
CACCAATCCA CTATTCATTT CAGAACAGCT GGAAGATGCC TCACTAGAGG GATGGATGTG 
ATTTTTTCAG CAGAGCAGAC ATATGAGCTG ATGCGGTGCC TGGAAGACTT GGCCTCAGGG 
AGGCCAGAGT GTGGTGAACC TGATGCCCAG CGACTGCAGG ATGACGACAT AGAGACCACC 
AAGGTGGAGA TGCTGAGACT GGCTCTTGCC CGGAATCTTG CTCGGGCAAT TATAAGGGAA 
GGCTCACTGG AGGGTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101976.1
CDS	142..2022
Translation	MCFDWGPGEMLLCETSFNQTGKSEKVPSCPFIYIIRKDVDVYSQILRKLFNESHGIFVGLQKIEEELSGKSRKAQLVRVSKNYRSVIRACMEEMHQVAIAAKDPASGRQFSSQVSILSAMELIWNLCEILFIEVAPAGPLLLHLLDWVRLHVCEVDSLSADVLGGDNPSKHENFWDLVTVLVLQGRLDEARQMLAKEADANPSCAGMCRVLGDLMRTMPILSPGNTQTLTELELKWQHWREECERHLQDNTFAANPRLESLCKIMLGDEAALLEQKELLSNWYHFLVTRLLYSNPTVKPIDLHFYAQSSLDMFLGGESSPEPLDNILMAAFEFDIHQVIKECSIALSNWWFVAHLTDLLDHCRLLQSHNLYFGSNMREFLLLEYASGLFAHHSLWQLGVDYFDYCPELGRVSLELHIERIPLNTEQKALKVLRICEQRQMTEQVKSICKILAMKAVRNNRLGSALSWSIRAKDAAFATLVSDRFLRDYCERGCFSDLDLIDNLGSAMMLSDRLTFLAGFCSHTEAHLRLSSTVYTCPRLEHQSTIHFRTAGRCLTRGMDVIFSAEQTYELMRCLEDLASGRPECGEPDAQRLQDDDIETTKVEMLRLALARNLARAIIREGSLEGS

Target ORF information:

RefSeq Version	XM_030246116.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nucleoporin 85 (Nup85), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246116.1

ORF Insert Sequence:

ATGTGTTTTG ACTGGGGCCC CGGCGAGATG CTTCTGTGTG AAACTTCCTT CAACCAAACA 
GGCAAATCAG AGAAGGTGCC GAGCTGCCCT TTTATCTACA TCATACGGAA AGATGTGGAT 
GTTTACTCTC AAATTTTGAG AAAACTCTTC AATGAATCCC ATGGAATCTT TGTTGGCCTG 
CAAAAAATTG AGGAAGAACT GTCGGGGAAG TCCAGGAAAG CTCAATTGGT TCGAGTGAGT 
AAAAATTACC GTTCAGTCAT ACGGGCCTGT ATGGAAGAAA TGCATCAGGT TGCAATTGCT 
GCTAAAGATC CAGCCAGTGG CCGGCAGTTC AGCAGCCAGG TCTCCATTTT GTCAGCCATG 
GAGCTCATTT GGAACCTGTG TGAGATTCTC TTTATTGAAG TAGCACCAGC TGGCCCTCTC 
CTCCTTCACC TTCTTGACTG GGTCCGACTG CACGTGTGCG AGGTGGACAG TTTGTCGGCA 
GATGTCCTGG GCGGTGACAA CCCAAGCAAG CATGAAAACT TCTGGGACCT GGTGACTGTT 
CTGGTGCTTC AGGGCCGGCT CGATGAGGCA CGGCAGATGC TAGCCAAAGA AGCTGATGCC 
AACCCCTCTT GTGCAGGCAT GTGCCGCGTC CTTGGGGACC TGATGAGGAC AATGCCCATT 
CTCAGCCCTG GCAATACTCA GACACTGACA GAGTTGGAGC TGAAGTGGCA GCACTGGCGT 
GAGGAGTGTG AAAGACACTT ACAAGACAAC ACATTTGCAG CCAACCCCCG TCTGGAGTCT 
CTCTGCAAGA TCATGCTGGG AGATGAGGCC GCCTTGTTGG AGCAGAAGGA GCTTCTGAGC 
AACTGGTACC ATTTCTTAGT GACGAGGCTG CTGTACTCTA ACCCCACAGT GAAGCCCATT 
GACCTGCACT TCTATGCCCA GTCCAGCCTA GACATGTTTC TTGGAGGTGA GAGCAGTCCA 
GAACCACTGG ACAACATCTT GATGGCGGCC TTTGAGTTCG ACATTCACCA GGTGATCAAA 
GAGTGCAGCA TCGCCCTGAG CAACTGGTGG TTCGTAGCTC ACCTCACAGA CCTTTTGGAT 
CACTGCAGAC TCCTCCAGTC ACACAATCTC TATTTTGGTT CTAACATGAG AGAATTCCTC 
CTGCTGGAGT ACGCCTCAGG ACTGTTTGCT CACCACAGCC TGTGGCAGCT GGGGGTGGAC 
TACTTTGATT ACTGCCCGGA GCTAGGCCGA GTTTCCTTGG AGCTGCACAT TGAGCGGATT 
CCTCTCAACA CAGAGCAGAA AGCCTTGAAG GTGCTGAGGA TTTGTGAGCA GCGGCAGATG 
ACTGAGCAAG TTAAAAGCAT CTGTAAGATC TTGGCCATGA AGGCTGTTCG TAATAACCGC 
TTGGGCTCAG CACTCTCCTG GAGCATCCGT GCCAAAGATG CTGCCTTTGC CACACTCGTA 
TCTGACAGAT TCCTCCGGGA TTACTGTGAG AGAGGCTGCT TTTCTGACTT GGATCTCATT 
GACAATCTGG GGTCAGCCAT GATGCTCAGT GATCGACTGA CGTTTCTGGC TGGCTTCTGC 
AGCCATACAG AAGCTCATTT ACGCCTAAGC AGTACCGTGT ACACGTGTCC AAGGCTCGAG 
CACCAATCCA CTATTCATTT CAGAACAGCT GGAAGATGCC TCACTAGAGG GATGGATGTG 
ATTTTTTCAG CAGAGCAGAC ATATGAGCTG ATGCGGTGCC TGGAAGACTT GGCCTCAGGG 
AGGCCAGAGT GTGGTGAACC TGATGCCCAG CGACTGCAGG ATGACGACAT AGAGACCACC 
AAGGTGGAGA TGCTGAGACT GGCTCTTGCC CGGAATCTTG CTCGGGCAAT TATAAGGGAA 
GGCTCACTGG AGGGTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Venus trap in the mouse embryo reveals distinct molecular dynamics underlying specification of first embryonic lineages.
EMBO reports16(8)1005-21(2015 Aug)
Dietrich JE,Panavaite L,Gunther S,Wennekamp S,Groner AC,Pigge A,Salvenmoser S,Trono D,Hufnagel L,Hiiragi T

Pivotal function for cytoplasmic protein FROUNT in CCR2-mediated monocyte chemotaxis.
Nature immunology6(8)827-35(2005 Aug)
Terashima Y,Onai N,Murai M,Enomoto M,Poonpiriya V,Hamada T,Motomura K,Suwa M,Ezaki T,Haga T,Kanegasaki S,Matsushima K

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Nup85 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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