Pnpla1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pnpla1
Entrez Gene ID	433091
Full Name	patatin-like phospholipase domain containing 1
General protein information	Preferred Names patatin-like phospholipase domain containing 1 Names patatin-like phospholipase domain-containing protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 A3.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006524581.3	XP_006524644.1	patatin-like phospholipase domain-containing protein 1 isoform X1
XM_006524582.4	XP_006524645.1	patatin-like phospholipase domain-containing protein 1 isoform X1
XM_006524585.2	XP_006524648.1	patatin-like phospholipase domain-containing protein 1 isoform X2
NM_001034885.3	NP_001030057.1	patatin-like phospholipase domain-containing protein 1
XM_006524585.3	XP_006524648.1	patatin-like phospholipase domain-containing protein 1 isoform X2
XM_006524582.3	XP_006524645.1	patatin-like phospholipase domain-containing protein 1 isoform X1
XM_006524581.4	XP_006524644.1	patatin-like phospholipase domain-containing protein 1 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PNPLA1	NP_001092670.1
Mus musculus (house mouse)	Pnpla1	NP_001030057.1
Canis lupus familiaris (dog)	PNPLA1	XP_005627325.1
Gallus gallus (chicken)	PNPLA1	XP_425818.2
Xenopus tropicalis (tropical clawed frog)	LOC100497390	XP_002938479.2
Homo sapiens (human)	PNPLA1	NP_001139189.2
Pan troglodytes (chimpanzee)	PNPLA1	XP_001172868.2
Rattus norvegicus (Norway rat)	Pnpla1	NP_001178770.1

Gene Ontology
Bibliography

Related articles in PubMed

PNPLA1 defects in patients with autosomal recessive congenital ichthyosis and KO mice sustain PNPLA1 irreplaceable function in epidermal omega-O-acylceramide synthesis and skin permeability barrier.
Pichery M, Huchenq A, Sandhoff R, Severino-Freire M, Zaafouri S, Op?lka L, Levade T, Soldan V, Bertrand-Michel J, Lhuillier E, Serre G, Maruani A, Mazereeuw-Hautier J, Jonca N
Human molecular genetics26(10)1787-1800(2017 05)

PNPLA1 has a crucial role in skin barrier function by directing acylceramide biosynthesis.
Hirabayashi T, Anjo T, Kaneko A, Senoo Y, Shibata A, Takama H, Yokoyama K, Nishito Y, Ono T, Taya C, Muramatsu K, Fukami K, Mu?oz-Garcia A, Brash AR, Ikeda K, Arita M, Akiyama M, Murakami M
Nature communications814609(2017 Mar)

PNPLA1 Deficiency in Mice and Humans?Leads to a Defect in the Synthesis?of Omega-O-Acylceramides.
Grond S, Eichmann TO, Dubrac S, Kolb D, Schmuth M, Fischer J, Crumrine D, Elias PM, Haemmerle G, Zechner R, Lass A, Radner FP
The Journal of investigative dermatology137(2)394-402(2017 Feb)

Identification mouse patatin-like phospholipase domain containing protein 1 as a skin-specific and membrane-associated protein.
Chang PA, Han LP, Sun LX, Huang FF
Gene591(2)344-50(2016 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

an essential role of PNPLA1 in the synthesis of omega-O-AcylCers in human and murine skin.
Title: PNPLA1 Deficiency in Mice and Humans?Leads to a Defect in the Synthesis?of Omega-O-Acylceramides.

results indicate PNPLA1 is a skin-specific and membrane-associated protein, suggesting that it may mainly play a role in the skin
Title: Identification mouse patatin-like phospholipase domain containing protein 1 as a skin-specific and membrane-associated protein.

The following Pnpla1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pnpla1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu12782	NM_001034885.3 Latest version!	Mus musculus patatin-like phospholipase domain containing 1 (Pnpla1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$349.30 $499.00
OMu53807	XM_006524585.3 Latest version!	Mus musculus patatin-like phospholipase domain containing 1 (Pnpla1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu53806	XM_006524582.4 Latest version!	Mus musculus patatin-like phospholipase domain containing 1 (Pnpla1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu53806	XM_006524581.4 Latest version!	Mus musculus patatin-like phospholipase domain containing 1 (Pnpla1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu53807	XM_006524585.2	Mus musculus patatin-like phospholipase domain containing 1 (Pnpla1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu53806	XM_006524582.3	Mus musculus patatin-like phospholipase domain containing 1 (Pnpla1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu53806	XM_006524581.3	Mus musculus patatin-like phospholipase domain containing 1 (Pnpla1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pnpla1 ( NM_001034885.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001030057.1 Mus musculus patatin-like phospholipase domain containing 1 (Pnpla1), mRNA.

Price & Availability↑

CloneID	OMu12782
Clone ID Related Accession (Same CDS sequence)	NM_001034885.3
Accession Version	NM_001034885.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC140278.2. On May 2, 2007 this sequence version replaced NM_001034885.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK132521.1, BC139043.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849377 [ECO:0000348] ##Evidence-Data-END##

ATGGACGAAC AGGTGTTCAA AGGAGACCCA GATACCCCTC ATTCCATCTC CTTCTCCGGG 
AGCGGGTTCC TTTCCTACTA CCAGGCTGGG GCCGTGGATG CTCTGCGAGA TCTGGCCCCG 
AGGATGCTGG ACACAGCCCA TCGCTTTGCA GGGACGTCAG CGGGAGCGGT GATTGCGGCC 
TTGGTCGTCT GCGGGATTGA GATGGAGAAA TACCTCAGAG TGCTGAATAT GGGCCTGGCG 
GAGGTGAAGA AGTTCTTCCT GGGACCTCTG TCTCCGTCCT GCAAGATGGT ACAGATGATG 
CGACAGTTTT TGTACGATGT GCTACCGGAG GACTCCTACA AGTTCGCCAC CGGGAAGCTG 
CATGTGAGCC TCACCCGAGT CACGGACGGA GAGAACGTCG TGGTTTCCGA GTACCGATCC 
AAGGAAGAGC TCATCGAGGC CCTGTATTGC AGCTGCTTTG TTCCTGTTTA CTGTGGCTTC 
ATCCCCCCAA CGTATCGGGG AGAGAGATAC ATCGACGGTG GCTTCACAAG CATGCAGCCC 
TGTTCCTTCT GGACAGACTC CATCACCATC TCCACCTTCA GCAGCCAGCA GGACATCTGT 
CCGAGAGACT GCCCCACCAT CTTCCATGAC TTCCGAATGT TCAACTTCTC CTTCCAGTTC 
TCCCTGGAGA ATATCACCCG CATGACACAT GCGCTGTTTC CCCCGGACCT GGTGATTCTG 
CAGGAATATT ACTATCGGGG ATACAATGAT GCTGTCTCAT ACCTGCGGAG ACTGAATGCA 
GCGTACCTTG ACTCTCCCAG CAAGAGAGTG ATTTTCCCGA GGGTTGAAGT ATACTGCCAG 
ATAGAGGTCG CCCTTGGCCA TGAGCCCCCA CCTCCGAGTC TGCAGAACCT GCCAGCCCTG 
AGGAGAAGCC CAGCAGACTC CTCACAAACC CATGCACAGG GGTCTCCCAA AAAGGACAGA 
AAGGACAGCC ATTCCTCAGC CGCCCCCTCA GTGCAGACAC CTGAATCTGG GTGCAAGGAG 
TCTGTGGAAT CACCCGTGTC ACTACGGGTC TCTATATCCA AGCAACCATC TGTATCGCCA 
TTATCCCCAG CCCAGCCGGT CCCAGTAATG AGGCCCACTG GCCCCAGGGA CAGTTGCCCA 
ATAAATGTTC AAACTCCAAA CCCGGAGCGA GGAGTGAAGG GTGCCCTGGA CTCTGCCACA 
GAACGAGGAA TGAAGGATGC TCTGGCCTCT GCCACTGACG AGCAGAGCAC AACTACCTTG 
CCACCTGTGT TGCTCCCAGC TGCAGACTCA CGAGGCTCAA AGACTGGATC CTCTGTGCCT 
ATTGGGTCAC CCGAGTCCCC TAGACTGCTG CTCAGATCTT CCCAGGGAGC CACAGCATCC 
AGGGCAACAC TTGGACTTCC GCCCCTAAGT CCTTCAACAC CACCTGCTGG GCCACCTGTG 
GAGGATCTAG GCCCAGAACG ACCCACAGCT ACAGGGTCTC CCGCCTTATC ACAGCTGACA 
GGCTCGGCAG CACCGGGTAC CGGGAAGAAA GCCCCCCACA AGCCTCTGCT GGTGGAGGGT 
CCTGGTGAGG ATTCAAATAC GGCAAAAACG ATGTTCAAGA GGAAGCAGAA GACCAATGCC 
ACCAGAGAGT GCTTCCACCG AAATGCTCAG TCCAAGAAGC CGGCTAGCAA ACTGAAGTCT 
GCACCTTGTC CACTTAACTT TCCTGTACTC CCCAAGAGAG TTTGGGTCAC CTATAAACCT 
CATCCCAGCC GGATCCAAGA CTACAGCTAC CCCGAGGGAG TGAGTGGCCA GAACTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001030057.1
CDS	457..2256
Translation	MDEQVFKGDPDTPHSISFSGSGFLSYYQAGAVDALRDLAPRMLDTAHRFAGTSAGAVIAALVVCGIEMEKYLRVLNMGLAEVKKFFLGPLSPSCKMVQMMRQFLYDVLPEDSYKFATGKLHVSLTRVTDGENVVVSEYRSKEELIEALYCSCFVPVYCGFIPPTYRGERYIDGGFTSMQPCSFWTDSITISTFSSQQDICPRDCPTIFHDFRMFNFSFQFSLENITRMTHALFPPDLVILQEYYYRGYNDAVSYLRRLNAAYLDSPSKRVIFPRVEVYCQIEVALGHEPPPPSLQNLPALRRSPADSSQTHAQGSPKKDRKDSHSSAAPSVQTPESGCKESVESPVSLRVSISKQPSVSPLSPAQPVPVMRPTGPRDSCPINVQTPNPERGVKGALDSATERGMKDALASATDEQSTTTLPPVLLPAADSRGSKTGSSVPIGSPESPRLLLRSSQGATASRATLGLPPLSPSTPPAGPPVEDLGPERPTATGSPALSQLTGSAAPGTGKKAPHKPLLVEGPGEDSNTAKTMFKRKQKTNATRECFHRNAQSKKPASKLKSAPCPLNFPVLPKRVWVTYKPHPSRIQDYSYPEGVSGQNS

Target ORF information:

RefSeq Version	NM_001034885.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 1 (Pnpla1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034885.3

ORF Insert Sequence:

ATGGACGAAC AGGTGTTCAA AGGAGACCCA GATACCCCTC ATTCCATCTC CTTCTCCGGG 
AGCGGGTTCC TTTCCTACTA CCAGGCTGGG GCCGTGGATG CTCTGCGAGA TCTGGCCCCG 
AGGATGCTGG ACACAGCCCA TCGCTTTGCA GGGACGTCAG CGGGAGCGGT GATTGCGGCC 
TTGGTCGTCT GCGGGATTGA GATGGAGAAA TACCTCAGAG TGCTGAATAT GGGCCTGGCG 
GAGGTGAAGA AGTTCTTCCT GGGACCTCTG TCTCCGTCCT GCAAGATGGT ACAGATGATG 
CGACAGTTTT TGTACGATGT GCTACCGGAG GACTCCTACA AGTTCGCCAC CGGGAAGCTG 
CATGTGAGCC TCACCCGAGT CACGGACGGA GAGAACGTCG TGGTTTCCGA GTACCGATCC 
AAGGAAGAGC TCATCGAGGC CCTGTATTGC AGCTGCTTTG TTCCTGTTTA CTGTGGCTTC 
ATCCCCCCAA CGTATCGGGG AGAGAGATAC ATCGACGGTG GCTTCACAAG CATGCAGCCC 
TGTTCCTTCT GGACAGACTC CATCACCATC TCCACCTTCA GCAGCCAGCA GGACATCTGT 
CCGAGAGACT GCCCCACCAT CTTCCATGAC TTCCGAATGT TCAACTTCTC CTTCCAGTTC 
TCCCTGGAGA ATATCACCCG CATGACACAT GCGCTGTTTC CCCCGGACCT GGTGATTCTG 
CAGGAATATT ACTATCGGGG ATACAATGAT GCTGTCTCAT ACCTGCGGAG ACTGAATGCA 
GCGTACCTTG ACTCTCCCAG CAAGAGAGTG ATTTTCCCGA GGGTTGAAGT ATACTGCCAG 
ATAGAGGTCG CCCTTGGCCA TGAGCCCCCA CCTCCGAGTC TGCAGAACCT GCCAGCCCTG 
AGGAGAAGCC CAGCAGACTC CTCACAAACC CATGCACAGG GGTCTCCCAA AAAGGACAGA 
AAGGACAGCC ATTCCTCAGC CGCCCCCTCA GTGCAGACAC CTGAATCTGG GTGCAAGGAG 
TCTGTGGAAT CACCCGTGTC ACTACGGGTC TCTATATCCA AGCAACCATC TGTATCGCCA 
TTATCCCCAG CCCAGCCGGT CCCAGTAATG AGGCCCACTG GCCCCAGGGA CAGTTGCCCA 
ATAAATGTTC AAACTCCAAA CCCGGAGCGA GGAGTGAAGG GTGCCCTGGA CTCTGCCACA 
GAACGAGGAA TGAAGGATGC TCTGGCCTCT GCCACTGACG AGCAGAGCAC AACTACCTTG 
CCACCTGTGT TGCTCCCAGC TGCAGACTCA CGAGGCTCAA AGACTGGATC CTCTGTGCCT 
ATTGGGTCAC CCGAGTCCCC TAGACTGCTG CTCAGATCTT CCCAGGGAGC CACAGCATCC 
AGGGCAACAC TTGGACTTCC GCCCCTAAGT CCTTCAACAC CACCTGCTGG GCCACCTGTG 
GAGGATCTAG GCCCAGAACG ACCCACAGCT ACAGGGTCTC CCGCCTTATC ACAGCTGACA 
GGCTCGGCAG CACCGGGTAC CGGGAAGAAA GCCCCCCACA AGCCTCTGCT GGTGGAGGGT 
CCTGGTGAGG ATTCAAATAC GGCAAAAACG ATGTTCAAGA GGAAGCAGAA GACCAATGCC 
ACCAGAGAGT GCTTCCACCG AAATGCTCAG TCCAAGAAGC CGGCTAGCAA ACTGAAGTCT 
GCACCTTGTC CACTTAACTT TCCTGTACTC CCCAAGAGAG TTTGGGTCAC CTATAAACCT 
CATCCCAGCC GGATCCAAGA CTACAGCTAC CCCGAGGGAG TGAGTGGCCA GAACTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla1 ( XM_006524581.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524644.1 Mus musculus patatin-like phospholipase domain containing 1 (Pnpla1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53806
Clone ID Related Accession (Same CDS sequence)	XM_006524581.3 , XM_006524582.4 , XM_006524582.3 , XM_006524581.4
Accession Version	XM_006524581.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524581.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCTGG CGGAGGTGAA GAAGTTCTTC CTGGGACCTC TGTCTCCGTC CTGCAAGATG 
GTACAGATGA TGCGACAGTT TTTGTACGAT GTGCTACCGG AGGACTCCTA CAAGTTCGCC 
ACCGGGAAGC TGCATGTGAG CCTCACCCGA GTCACGGACG GAGAGAACGT CGTGGTTTCC 
GAGTACCGAT CCAAGGAAGA GCTCATCGAG GCCCTGTATT GCAGCTGCTT TGTTCCTGTT 
TACTGTGGCT TCATCCCCCC AACGTATCGG GGAGAGAGAT ACATCGACGG TGGCTTCACA 
AGCATGCAGC CCTGTTCCTT CTGGACAGAC TCCATCACCA TCTCCACCTT CAGCAGCCAG 
CAGGACATCT GTCCGAGAGA CTGCCCCACC ATCTTCCATG ACTTCCGAAT GTTCAACTTC 
TCCTTCCAGT TCTCCCTGGA GAATATCACC CGCATGACAC ATGCGCTGTT TCCCCCGGAC 
CTGGTGATTC TGCAGGAATA TTACTATCGG GGATACAATG ATGCTGTCTC ATACCTGCGG 
AGACTGAATG CAGCGTACCT TGACTCTCCC AGCAAGAGAG TGATTTTCCC GAGGGTTGAA 
GTATACTGCC AGATAGAGGT CGCCCTTGGC CATGAGCCCC CACCTCCGAG TCTGCAGAAC 
CTGCCAGCCC TGAGGAGAAG CCCAGCAGAC TCCTCACAAA CCCATGCACA GGGGTCTCCC 
AAAAAGGACA GAAAGGACAG CCATTCCTCA GCCGCCCCCT CAGTGCAGAC ACCTGAATCT 
GGGTGCAAGG AGTCTGTGGA ATCACCCGTG TCACTACGGG TCTCTATATC CAAGCAACCA 
TCTGTATCGC CATTATCCCC AGCCCAGCCG GTCCCAGTAA TGAGGCCCAC TGGCCCCAGG 
GACAGTTGCC CAATAAATGT TCAAACTCCA AACCCGGAGC GAGGAGTGAA GGGTGCCCTG 
GACTCTGCCA CAGAACGAGG AATGAAGGAT GCTCTGGCCT CTGCCACTGA CGAGCAGAGC 
ACAACTACCT TGCCACCTGT GTTGCTCCCA GCTGCAGACT CACGAGGCTC AAAGACTGGA 
TCCTCTGTGC CTATTGGGTC ACCCGAGTCC CCTAGACTGC TGCTCAGATC TTCCCAGGGA 
GCCACAGCAT CCAGGGCAAC ACTTGGACTT CCGCCCCTAA GTCCTTCAAC ACCACCTGCT 
GGGCCACCTG TGGAGGATCT AGGCCCAGAA CGACCCACAG CTACAGGGTC TCCCGCCTTA 
TCACAGCTGA CAGGCTCGGC AGCACCGGGT ACCGGGAAGA AAGCCCCCCA CAAGCCTCTG 
CTGGTGGAGG GTCCTGGTGA GGATTCAAAT ACGGCAAAAA CGATGTTCAA GAGGAAGCAG 
AAGACCAATG CCACCAGAGA GTGCTTCCAC CGAAATGCTC AGTCCAAGAA GCCGGCTAGC 
AAACTGAAGT CTGCACCTTG TCCACTTAAC TTTCCTGTAC TCCCCAAGAG AGTTTGGGTC 
ACCTATAAAC CTCATCCCAG CCGGATCCAA GACTACAGCT ACCCCGAGGG AGTGAGTGGC 
CAGAACTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524644.1
CDS	788..2359
Translation	MGLAEVKKFFLGPLSPSCKMVQMMRQFLYDVLPEDSYKFATGKLHVSLTRVTDGENVVVSEYRSKEELIEALYCSCFVPVYCGFIPPTYRGERYIDGGFTSMQPCSFWTDSITISTFSSQQDICPRDCPTIFHDFRMFNFSFQFSLENITRMTHALFPPDLVILQEYYYRGYNDAVSYLRRLNAAYLDSPSKRVIFPRVEVYCQIEVALGHEPPPPSLQNLPALRRSPADSSQTHAQGSPKKDRKDSHSSAAPSVQTPESGCKESVESPVSLRVSISKQPSVSPLSPAQPVPVMRPTGPRDSCPINVQTPNPERGVKGALDSATERGMKDALASATDEQSTTTLPPVLLPAADSRGSKTGSSVPIGSPESPRLLLRSSQGATASRATLGLPPLSPSTPPAGPPVEDLGPERPTATGSPALSQLTGSAAPGTGKKAPHKPLLVEGPGEDSNTAKTMFKRKQKTNATRECFHRNAQSKKPASKLKSAPCPLNFPVLPKRVWVTYKPHPSRIQDYSYPEGVSGQNS

Target ORF information:

RefSeq Version	XM_006524581.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 1 (Pnpla1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524581.3

ORF Insert Sequence:

ATGGGCCTGG CGGAGGTGAA GAAGTTCTTC CTGGGACCTC TGTCTCCGTC CTGCAAGATG 
GTACAGATGA TGCGACAGTT TTTGTACGAT GTGCTACCGG AGGACTCCTA CAAGTTCGCC 
ACCGGGAAGC TGCATGTGAG CCTCACCCGA GTCACGGACG GAGAGAACGT CGTGGTTTCC 
GAGTACCGAT CCAAGGAAGA GCTCATCGAG GCCCTGTATT GCAGCTGCTT TGTTCCTGTT 
TACTGTGGCT TCATCCCCCC AACGTATCGG GGAGAGAGAT ACATCGACGG TGGCTTCACA 
AGCATGCAGC CCTGTTCCTT CTGGACAGAC TCCATCACCA TCTCCACCTT CAGCAGCCAG 
CAGGACATCT GTCCGAGAGA CTGCCCCACC ATCTTCCATG ACTTCCGAAT GTTCAACTTC 
TCCTTCCAGT TCTCCCTGGA GAATATCACC CGCATGACAC ATGCGCTGTT TCCCCCGGAC 
CTGGTGATTC TGCAGGAATA TTACTATCGG GGATACAATG ATGCTGTCTC ATACCTGCGG 
AGACTGAATG CAGCGTACCT TGACTCTCCC AGCAAGAGAG TGATTTTCCC GAGGGTTGAA 
GTATACTGCC AGATAGAGGT CGCCCTTGGC CATGAGCCCC CACCTCCGAG TCTGCAGAAC 
CTGCCAGCCC TGAGGAGAAG CCCAGCAGAC TCCTCACAAA CCCATGCACA GGGGTCTCCC 
AAAAAGGACA GAAAGGACAG CCATTCCTCA GCCGCCCCCT CAGTGCAGAC ACCTGAATCT 
GGGTGCAAGG AGTCTGTGGA ATCACCCGTG TCACTACGGG TCTCTATATC CAAGCAACCA 
TCTGTATCGC CATTATCCCC AGCCCAGCCG GTCCCAGTAA TGAGGCCCAC TGGCCCCAGG 
GACAGTTGCC CAATAAATGT TCAAACTCCA AACCCGGAGC GAGGAGTGAA GGGTGCCCTG 
GACTCTGCCA CAGAACGAGG AATGAAGGAT GCTCTGGCCT CTGCCACTGA CGAGCAGAGC 
ACAACTACCT TGCCACCTGT GTTGCTCCCA GCTGCAGACT CACGAGGCTC AAAGACTGGA 
TCCTCTGTGC CTATTGGGTC ACCCGAGTCC CCTAGACTGC TGCTCAGATC TTCCCAGGGA 
GCCACAGCAT CCAGGGCAAC ACTTGGACTT CCGCCCCTAA GTCCTTCAAC ACCACCTGCT 
GGGCCACCTG TGGAGGATCT AGGCCCAGAA CGACCCACAG CTACAGGGTC TCCCGCCTTA 
TCACAGCTGA CAGGCTCGGC AGCACCGGGT ACCGGGAAGA AAGCCCCCCA CAAGCCTCTG 
CTGGTGGAGG GTCCTGGTGA GGATTCAAAT ACGGCAAAAA CGATGTTCAA GAGGAAGCAG 
AAGACCAATG CCACCAGAGA GTGCTTCCAC CGAAATGCTC AGTCCAAGAA GCCGGCTAGC 
AAACTGAAGT CTGCACCTTG TCCACTTAAC TTTCCTGTAC TCCCCAAGAG AGTTTGGGTC 
ACCTATAAAC CTCATCCCAG CCGGATCCAA GACTACAGCT ACCCCGAGGG AGTGAGTGGC 
CAGAACTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla1 ( XM_006524582.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524645.1 Mus musculus patatin-like phospholipase domain containing 1 (Pnpla1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu53806
Clone ID Related Accession (Same CDS sequence)	XM_006524581.3 , XM_006524582.4 , XM_006524582.3 , XM_006524581.4
Accession Version	XM_006524582.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524582.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCTGG CGGAGGTGAA GAAGTTCTTC CTGGGACCTC TGTCTCCGTC CTGCAAGATG 
GTACAGATGA TGCGACAGTT TTTGTACGAT GTGCTACCGG AGGACTCCTA CAAGTTCGCC 
ACCGGGAAGC TGCATGTGAG CCTCACCCGA GTCACGGACG GAGAGAACGT CGTGGTTTCC 
GAGTACCGAT CCAAGGAAGA GCTCATCGAG GCCCTGTATT GCAGCTGCTT TGTTCCTGTT 
TACTGTGGCT TCATCCCCCC AACGTATCGG GGAGAGAGAT ACATCGACGG TGGCTTCACA 
AGCATGCAGC CCTGTTCCTT CTGGACAGAC TCCATCACCA TCTCCACCTT CAGCAGCCAG 
CAGGACATCT GTCCGAGAGA CTGCCCCACC ATCTTCCATG ACTTCCGAAT GTTCAACTTC 
TCCTTCCAGT TCTCCCTGGA GAATATCACC CGCATGACAC ATGCGCTGTT TCCCCCGGAC 
CTGGTGATTC TGCAGGAATA TTACTATCGG GGATACAATG ATGCTGTCTC ATACCTGCGG 
AGACTGAATG CAGCGTACCT TGACTCTCCC AGCAAGAGAG TGATTTTCCC GAGGGTTGAA 
GTATACTGCC AGATAGAGGT CGCCCTTGGC CATGAGCCCC CACCTCCGAG TCTGCAGAAC 
CTGCCAGCCC TGAGGAGAAG CCCAGCAGAC TCCTCACAAA CCCATGCACA GGGGTCTCCC 
AAAAAGGACA GAAAGGACAG CCATTCCTCA GCCGCCCCCT CAGTGCAGAC ACCTGAATCT 
GGGTGCAAGG AGTCTGTGGA ATCACCCGTG TCACTACGGG TCTCTATATC CAAGCAACCA 
TCTGTATCGC CATTATCCCC AGCCCAGCCG GTCCCAGTAA TGAGGCCCAC TGGCCCCAGG 
GACAGTTGCC CAATAAATGT TCAAACTCCA AACCCGGAGC GAGGAGTGAA GGGTGCCCTG 
GACTCTGCCA CAGAACGAGG AATGAAGGAT GCTCTGGCCT CTGCCACTGA CGAGCAGAGC 
ACAACTACCT TGCCACCTGT GTTGCTCCCA GCTGCAGACT CACGAGGCTC AAAGACTGGA 
TCCTCTGTGC CTATTGGGTC ACCCGAGTCC CCTAGACTGC TGCTCAGATC TTCCCAGGGA 
GCCACAGCAT CCAGGGCAAC ACTTGGACTT CCGCCCCTAA GTCCTTCAAC ACCACCTGCT 
GGGCCACCTG TGGAGGATCT AGGCCCAGAA CGACCCACAG CTACAGGGTC TCCCGCCTTA 
TCACAGCTGA CAGGCTCGGC AGCACCGGGT ACCGGGAAGA AAGCCCCCCA CAAGCCTCTG 
CTGGTGGAGG GTCCTGGTGA GGATTCAAAT ACGGCAAAAA CGATGTTCAA GAGGAAGCAG 
AAGACCAATG CCACCAGAGA GTGCTTCCAC CGAAATGCTC AGTCCAAGAA GCCGGCTAGC 
AAACTGAAGT CTGCACCTTG TCCACTTAAC TTTCCTGTAC TCCCCAAGAG AGTTTGGGTC 
ACCTATAAAC CTCATCCCAG CCGGATCCAA GACTACAGCT ACCCCGAGGG AGTGAGTGGC 
CAGAACTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524645.1
CDS	753..2324
Translation	MGLAEVKKFFLGPLSPSCKMVQMMRQFLYDVLPEDSYKFATGKLHVSLTRVTDGENVVVSEYRSKEELIEALYCSCFVPVYCGFIPPTYRGERYIDGGFTSMQPCSFWTDSITISTFSSQQDICPRDCPTIFHDFRMFNFSFQFSLENITRMTHALFPPDLVILQEYYYRGYNDAVSYLRRLNAAYLDSPSKRVIFPRVEVYCQIEVALGHEPPPPSLQNLPALRRSPADSSQTHAQGSPKKDRKDSHSSAAPSVQTPESGCKESVESPVSLRVSISKQPSVSPLSPAQPVPVMRPTGPRDSCPINVQTPNPERGVKGALDSATERGMKDALASATDEQSTTTLPPVLLPAADSRGSKTGSSVPIGSPESPRLLLRSSQGATASRATLGLPPLSPSTPPAGPPVEDLGPERPTATGSPALSQLTGSAAPGTGKKAPHKPLLVEGPGEDSNTAKTMFKRKQKTNATRECFHRNAQSKKPASKLKSAPCPLNFPVLPKRVWVTYKPHPSRIQDYSYPEGVSGQNS

Target ORF information:

RefSeq Version	XM_006524582.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 1 (Pnpla1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524582.3

ORF Insert Sequence:

ATGGGCCTGG CGGAGGTGAA GAAGTTCTTC CTGGGACCTC TGTCTCCGTC CTGCAAGATG 
GTACAGATGA TGCGACAGTT TTTGTACGAT GTGCTACCGG AGGACTCCTA CAAGTTCGCC 
ACCGGGAAGC TGCATGTGAG CCTCACCCGA GTCACGGACG GAGAGAACGT CGTGGTTTCC 
GAGTACCGAT CCAAGGAAGA GCTCATCGAG GCCCTGTATT GCAGCTGCTT TGTTCCTGTT 
TACTGTGGCT TCATCCCCCC AACGTATCGG GGAGAGAGAT ACATCGACGG TGGCTTCACA 
AGCATGCAGC CCTGTTCCTT CTGGACAGAC TCCATCACCA TCTCCACCTT CAGCAGCCAG 
CAGGACATCT GTCCGAGAGA CTGCCCCACC ATCTTCCATG ACTTCCGAAT GTTCAACTTC 
TCCTTCCAGT TCTCCCTGGA GAATATCACC CGCATGACAC ATGCGCTGTT TCCCCCGGAC 
CTGGTGATTC TGCAGGAATA TTACTATCGG GGATACAATG ATGCTGTCTC ATACCTGCGG 
AGACTGAATG CAGCGTACCT TGACTCTCCC AGCAAGAGAG TGATTTTCCC GAGGGTTGAA 
GTATACTGCC AGATAGAGGT CGCCCTTGGC CATGAGCCCC CACCTCCGAG TCTGCAGAAC 
CTGCCAGCCC TGAGGAGAAG CCCAGCAGAC TCCTCACAAA CCCATGCACA GGGGTCTCCC 
AAAAAGGACA GAAAGGACAG CCATTCCTCA GCCGCCCCCT CAGTGCAGAC ACCTGAATCT 
GGGTGCAAGG AGTCTGTGGA ATCACCCGTG TCACTACGGG TCTCTATATC CAAGCAACCA 
TCTGTATCGC CATTATCCCC AGCCCAGCCG GTCCCAGTAA TGAGGCCCAC TGGCCCCAGG 
GACAGTTGCC CAATAAATGT TCAAACTCCA AACCCGGAGC GAGGAGTGAA GGGTGCCCTG 
GACTCTGCCA CAGAACGAGG AATGAAGGAT GCTCTGGCCT CTGCCACTGA CGAGCAGAGC 
ACAACTACCT TGCCACCTGT GTTGCTCCCA GCTGCAGACT CACGAGGCTC AAAGACTGGA 
TCCTCTGTGC CTATTGGGTC ACCCGAGTCC CCTAGACTGC TGCTCAGATC TTCCCAGGGA 
GCCACAGCAT CCAGGGCAAC ACTTGGACTT CCGCCCCTAA GTCCTTCAAC ACCACCTGCT 
GGGCCACCTG TGGAGGATCT AGGCCCAGAA CGACCCACAG CTACAGGGTC TCCCGCCTTA 
TCACAGCTGA CAGGCTCGGC AGCACCGGGT ACCGGGAAGA AAGCCCCCCA CAAGCCTCTG 
CTGGTGGAGG GTCCTGGTGA GGATTCAAAT ACGGCAAAAA CGATGTTCAA GAGGAAGCAG 
AAGACCAATG CCACCAGAGA GTGCTTCCAC CGAAATGCTC AGTCCAAGAA GCCGGCTAGC 
AAACTGAAGT CTGCACCTTG TCCACTTAAC TTTCCTGTAC TCCCCAAGAG AGTTTGGGTC 
ACCTATAAAC CTCATCCCAG CCGGATCCAA GACTACAGCT ACCCCGAGGG AGTGAGTGGC 
CAGAACTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla1 ( XM_006524585.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524648.1 Mus musculus patatin-like phospholipase domain containing 1 (Pnpla1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu53807
Clone ID Related Accession (Same CDS sequence)	XM_006524585.2 , XM_006524585.3
Accession Version	XM_006524585.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006524585.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAAC AGGTGTTCAA AGGAGACCCA GATACCCCTC ATTCCATCTC CTTCTCCGGG 
AGCGGGTTCC TTTCCTACTA CCAGGCTGGG GCCGTGGATG CTCTGCGAGA TCTGGCCCCG 
AGGATGCTGG ACACAGCCCA TCGCTTTGCA GGGACGTCAG CGGGAGCGGT GATTGCGGCC 
TTGGTCGTCT GCGGGATTGA GATGGAGAAA TACCTCAGAG TGCTGAATAT GGGCCTGGCG 
GAGGTGAAGA AGTTCTTCCT GGGACCTCTG TCTCCGTCCT GCAAGATGGT ACAGATGATG 
CGACAGTTTT TGTACGATGT GCTACCGGAG GACTCCTACA AGTTCGCCAC CGGGAAGCTG 
CATGTGAGCC TCACCCGAGT CACGGACGGA GAGAACGTCG TGGTTTCCGA GTACCGATCC 
AAGGAAGAGC TCATCGAGGC CCTGTATTGC AGCTGCTTTG TTCCTGTTTA CTGTGGCTTC 
ATCCCCCCAA CGTATCGGGG AGAGAGATAC ATCGACGGTG GCTTCACAAG CATGCAGCCC 
TGTTCCTTCT GGACAGACTC CATCACCATC TCCACCTTCA GCAGCCAGCA GGACATCTGT 
CCGAGAGACT GCCCCACCAT CTTCCATGAC TTCCGAATGT TCAACTTCTC CTTCCAGTTC 
TCCCTGGAGA ATATCACCCG CATGACACAT GCGCTGTTTC CCCCGGACCT GGTGATTCTG 
CAGGAATATT ACTATCGGGG ATACAATGAT GCTGTCTCAT ACCTGCGGAG ACTGACTGCA 
GACTCACGAG GCTCAAAGAC TGGATCCTCT GTGCCTATTG GGTCACCCGA GTCCCCTAGA 
CTGCTGCTCA GATCTTCCCA GGGAGCCACA GCATCCAGGG CAACACTTGG ACTTCCGCCC 
CTAAGTCCTT CAACACCACC TGCTGGGCCA CCTGTGGAGG ATCTAGGCCC AGAACGACCC 
ACAGCTACAG GGTCTCCCGC CTTATCACAG CTGACAGGCT CGGCAGCACC GGGTACCGGG 
AAGAAAGCCC CCCACAAGCC TCTGCTGGTG GAGGGTCCTG GTGAGGATTC AAATACGGCA 
AAAACGATGT TCAAGAGGAA GCAGAAGACC AATGCCACCA GAGAGTGCTT CCACCGAAAT 
GCTCAGTCCA AGAAGCCGGC TAGCAAACTG AAGTCTGCAC CTTGTCCACT TAACTTTCCT 
GTACTCCCCA AGAGAGTTTG GGTCACCTAT AAACCTCATC CCAGCCGGAT CCAAGACTAC 
AGCTACCCCG AGGGAGTGAG TGGCCAGAAC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524648.1
CDS	903..2198
Translation	MDEQVFKGDPDTPHSISFSGSGFLSYYQAGAVDALRDLAPRMLDTAHRFAGTSAGAVIAALVVCGIEMEKYLRVLNMGLAEVKKFFLGPLSPSCKMVQMMRQFLYDVLPEDSYKFATGKLHVSLTRVTDGENVVVSEYRSKEELIEALYCSCFVPVYCGFIPPTYRGERYIDGGFTSMQPCSFWTDSITISTFSSQQDICPRDCPTIFHDFRMFNFSFQFSLENITRMTHALFPPDLVILQEYYYRGYNDAVSYLRRLTADSRGSKTGSSVPIGSPESPRLLLRSSQGATASRATLGLPPLSPSTPPAGPPVEDLGPERPTATGSPALSQLTGSAAPGTGKKAPHKPLLVEGPGEDSNTAKTMFKRKQKTNATRECFHRNAQSKKPASKLKSAPCPLNFPVLPKRVWVTYKPHPSRIQDYSYPEGVSGQNS

Target ORF information:

RefSeq Version	XM_006524585.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 1 (Pnpla1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524585.2

ORF Insert Sequence:

ATGGACGAAC AGGTGTTCAA AGGAGACCCA GATACCCCTC ATTCCATCTC CTTCTCCGGG 
AGCGGGTTCC TTTCCTACTA CCAGGCTGGG GCCGTGGATG CTCTGCGAGA TCTGGCCCCG 
AGGATGCTGG ACACAGCCCA TCGCTTTGCA GGGACGTCAG CGGGAGCGGT GATTGCGGCC 
TTGGTCGTCT GCGGGATTGA GATGGAGAAA TACCTCAGAG TGCTGAATAT GGGCCTGGCG 
GAGGTGAAGA AGTTCTTCCT GGGACCTCTG TCTCCGTCCT GCAAGATGGT ACAGATGATG 
CGACAGTTTT TGTACGATGT GCTACCGGAG GACTCCTACA AGTTCGCCAC CGGGAAGCTG 
CATGTGAGCC TCACCCGAGT CACGGACGGA GAGAACGTCG TGGTTTCCGA GTACCGATCC 
AAGGAAGAGC TCATCGAGGC CCTGTATTGC AGCTGCTTTG TTCCTGTTTA CTGTGGCTTC 
ATCCCCCCAA CGTATCGGGG AGAGAGATAC ATCGACGGTG GCTTCACAAG CATGCAGCCC 
TGTTCCTTCT GGACAGACTC CATCACCATC TCCACCTTCA GCAGCCAGCA GGACATCTGT 
CCGAGAGACT GCCCCACCAT CTTCCATGAC TTCCGAATGT TCAACTTCTC CTTCCAGTTC 
TCCCTGGAGA ATATCACCCG CATGACACAT GCGCTGTTTC CCCCGGACCT GGTGATTCTG 
CAGGAATATT ACTATCGGGG ATACAATGAT GCTGTCTCAT ACCTGCGGAG ACTGACTGCA 
GACTCACGAG GCTCAAAGAC TGGATCCTCT GTGCCTATTG GGTCACCCGA GTCCCCTAGA 
CTGCTGCTCA GATCTTCCCA GGGAGCCACA GCATCCAGGG CAACACTTGG ACTTCCGCCC 
CTAAGTCCTT CAACACCACC TGCTGGGCCA CCTGTGGAGG ATCTAGGCCC AGAACGACCC 
ACAGCTACAG GGTCTCCCGC CTTATCACAG CTGACAGGCT CGGCAGCACC GGGTACCGGG 
AAGAAAGCCC CCCACAAGCC TCTGCTGGTG GAGGGTCCTG GTGAGGATTC AAATACGGCA 
AAAACGATGT TCAAGAGGAA GCAGAAGACC AATGCCACCA GAGAGTGCTT CCACCGAAAT 
GCTCAGTCCA AGAAGCCGGC TAGCAAACTG AAGTCTGCAC CTTGTCCACT TAACTTTCCT 
GTACTCCCCA AGAGAGTTTG GGTCACCTAT AAACCTCATC CCAGCCGGAT CCAAGACTAC 
AGCTACCCCG AGGGAGTGAG TGGCCAGAAC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla1 ( XM_006524585.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524648.1 Mus musculus patatin-like phospholipase domain containing 1 (Pnpla1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu53807
Clone ID Related Accession (Same CDS sequence)	XM_006524585.2 , XM_006524585.3
Accession Version	XM_006524585.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524585.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAAC AGGTGTTCAA AGGAGACCCA GATACCCCTC ATTCCATCTC CTTCTCCGGG 
AGCGGGTTCC TTTCCTACTA CCAGGCTGGG GCCGTGGATG CTCTGCGAGA TCTGGCCCCG 
AGGATGCTGG ACACAGCCCA TCGCTTTGCA GGGACGTCAG CGGGAGCGGT GATTGCGGCC 
TTGGTCGTCT GCGGGATTGA GATGGAGAAA TACCTCAGAG TGCTGAATAT GGGCCTGGCG 
GAGGTGAAGA AGTTCTTCCT GGGACCTCTG TCTCCGTCCT GCAAGATGGT ACAGATGATG 
CGACAGTTTT TGTACGATGT GCTACCGGAG GACTCCTACA AGTTCGCCAC CGGGAAGCTG 
CATGTGAGCC TCACCCGAGT CACGGACGGA GAGAACGTCG TGGTTTCCGA GTACCGATCC 
AAGGAAGAGC TCATCGAGGC CCTGTATTGC AGCTGCTTTG TTCCTGTTTA CTGTGGCTTC 
ATCCCCCCAA CGTATCGGGG AGAGAGATAC ATCGACGGTG GCTTCACAAG CATGCAGCCC 
TGTTCCTTCT GGACAGACTC CATCACCATC TCCACCTTCA GCAGCCAGCA GGACATCTGT 
CCGAGAGACT GCCCCACCAT CTTCCATGAC TTCCGAATGT TCAACTTCTC CTTCCAGTTC 
TCCCTGGAGA ATATCACCCG CATGACACAT GCGCTGTTTC CCCCGGACCT GGTGATTCTG 
CAGGAATATT ACTATCGGGG ATACAATGAT GCTGTCTCAT ACCTGCGGAG ACTGACTGCA 
GACTCACGAG GCTCAAAGAC TGGATCCTCT GTGCCTATTG GGTCACCCGA GTCCCCTAGA 
CTGCTGCTCA GATCTTCCCA GGGAGCCACA GCATCCAGGG CAACACTTGG ACTTCCGCCC 
CTAAGTCCTT CAACACCACC TGCTGGGCCA CCTGTGGAGG ATCTAGGCCC AGAACGACCC 
ACAGCTACAG GGTCTCCCGC CTTATCACAG CTGACAGGCT CGGCAGCACC GGGTACCGGG 
AAGAAAGCCC CCCACAAGCC TCTGCTGGTG GAGGGTCCTG GTGAGGATTC AAATACGGCA 
AAAACGATGT TCAAGAGGAA GCAGAAGACC AATGCCACCA GAGAGTGCTT CCACCGAAAT 
GCTCAGTCCA AGAAGCCGGC TAGCAAACTG AAGTCTGCAC CTTGTCCACT TAACTTTCCT 
GTACTCCCCA AGAGAGTTTG GGTCACCTAT AAACCTCATC CCAGCCGGAT CCAAGACTAC 
AGCTACCCCG AGGGAGTGAG TGGCCAGAAC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524648.1
CDS	582..1877
Translation	MDEQVFKGDPDTPHSISFSGSGFLSYYQAGAVDALRDLAPRMLDTAHRFAGTSAGAVIAALVVCGIEMEKYLRVLNMGLAEVKKFFLGPLSPSCKMVQMMRQFLYDVLPEDSYKFATGKLHVSLTRVTDGENVVVSEYRSKEELIEALYCSCFVPVYCGFIPPTYRGERYIDGGFTSMQPCSFWTDSITISTFSSQQDICPRDCPTIFHDFRMFNFSFQFSLENITRMTHALFPPDLVILQEYYYRGYNDAVSYLRRLTADSRGSKTGSSVPIGSPESPRLLLRSSQGATASRATLGLPPLSPSTPPAGPPVEDLGPERPTATGSPALSQLTGSAAPGTGKKAPHKPLLVEGPGEDSNTAKTMFKRKQKTNATRECFHRNAQSKKPASKLKSAPCPLNFPVLPKRVWVTYKPHPSRIQDYSYPEGVSGQNS

Target ORF information:

RefSeq Version	XM_006524585.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 1 (Pnpla1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524585.3

ORF Insert Sequence:

ATGGACGAAC AGGTGTTCAA AGGAGACCCA GATACCCCTC ATTCCATCTC CTTCTCCGGG 
AGCGGGTTCC TTTCCTACTA CCAGGCTGGG GCCGTGGATG CTCTGCGAGA TCTGGCCCCG 
AGGATGCTGG ACACAGCCCA TCGCTTTGCA GGGACGTCAG CGGGAGCGGT GATTGCGGCC 
TTGGTCGTCT GCGGGATTGA GATGGAGAAA TACCTCAGAG TGCTGAATAT GGGCCTGGCG 
GAGGTGAAGA AGTTCTTCCT GGGACCTCTG TCTCCGTCCT GCAAGATGGT ACAGATGATG 
CGACAGTTTT TGTACGATGT GCTACCGGAG GACTCCTACA AGTTCGCCAC CGGGAAGCTG 
CATGTGAGCC TCACCCGAGT CACGGACGGA GAGAACGTCG TGGTTTCCGA GTACCGATCC 
AAGGAAGAGC TCATCGAGGC CCTGTATTGC AGCTGCTTTG TTCCTGTTTA CTGTGGCTTC 
ATCCCCCCAA CGTATCGGGG AGAGAGATAC ATCGACGGTG GCTTCACAAG CATGCAGCCC 
TGTTCCTTCT GGACAGACTC CATCACCATC TCCACCTTCA GCAGCCAGCA GGACATCTGT 
CCGAGAGACT GCCCCACCAT CTTCCATGAC TTCCGAATGT TCAACTTCTC CTTCCAGTTC 
TCCCTGGAGA ATATCACCCG CATGACACAT GCGCTGTTTC CCCCGGACCT GGTGATTCTG 
CAGGAATATT ACTATCGGGG ATACAATGAT GCTGTCTCAT ACCTGCGGAG ACTGACTGCA 
GACTCACGAG GCTCAAAGAC TGGATCCTCT GTGCCTATTG GGTCACCCGA GTCCCCTAGA 
CTGCTGCTCA GATCTTCCCA GGGAGCCACA GCATCCAGGG CAACACTTGG ACTTCCGCCC 
CTAAGTCCTT CAACACCACC TGCTGGGCCA CCTGTGGAGG ATCTAGGCCC AGAACGACCC 
ACAGCTACAG GGTCTCCCGC CTTATCACAG CTGACAGGCT CGGCAGCACC GGGTACCGGG 
AAGAAAGCCC CCCACAAGCC TCTGCTGGTG GAGGGTCCTG GTGAGGATTC AAATACGGCA 
AAAACGATGT TCAAGAGGAA GCAGAAGACC AATGCCACCA GAGAGTGCTT CCACCGAAAT 
GCTCAGTCCA AGAAGCCGGC TAGCAAACTG AAGTCTGCAC CTTGTCCACT TAACTTTCCT 
GTACTCCCCA AGAGAGTTTG GGTCACCTAT AAACCTCATC CCAGCCGGAT CCAAGACTAC 
AGCTACCCCG AGGGAGTGAG TGGCCAGAAC TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla1 ( XM_006524582.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524645.1 Mus musculus patatin-like phospholipase domain containing 1 (Pnpla1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu53806
Clone ID Related Accession (Same CDS sequence)	XM_006524581.3 , XM_006524582.4 , XM_006524582.3 , XM_006524581.4
Accession Version	XM_006524582.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524582.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCTGG CGGAGGTGAA GAAGTTCTTC CTGGGACCTC TGTCTCCGTC CTGCAAGATG 
GTACAGATGA TGCGACAGTT TTTGTACGAT GTGCTACCGG AGGACTCCTA CAAGTTCGCC 
ACCGGGAAGC TGCATGTGAG CCTCACCCGA GTCACGGACG GAGAGAACGT CGTGGTTTCC 
GAGTACCGAT CCAAGGAAGA GCTCATCGAG GCCCTGTATT GCAGCTGCTT TGTTCCTGTT 
TACTGTGGCT TCATCCCCCC AACGTATCGG GGAGAGAGAT ACATCGACGG TGGCTTCACA 
AGCATGCAGC CCTGTTCCTT CTGGACAGAC TCCATCACCA TCTCCACCTT CAGCAGCCAG 
CAGGACATCT GTCCGAGAGA CTGCCCCACC ATCTTCCATG ACTTCCGAAT GTTCAACTTC 
TCCTTCCAGT TCTCCCTGGA GAATATCACC CGCATGACAC ATGCGCTGTT TCCCCCGGAC 
CTGGTGATTC TGCAGGAATA TTACTATCGG GGATACAATG ATGCTGTCTC ATACCTGCGG 
AGACTGAATG CAGCGTACCT TGACTCTCCC AGCAAGAGAG TGATTTTCCC GAGGGTTGAA 
GTATACTGCC AGATAGAGGT CGCCCTTGGC CATGAGCCCC CACCTCCGAG TCTGCAGAAC 
CTGCCAGCCC TGAGGAGAAG CCCAGCAGAC TCCTCACAAA CCCATGCACA GGGGTCTCCC 
AAAAAGGACA GAAAGGACAG CCATTCCTCA GCCGCCCCCT CAGTGCAGAC ACCTGAATCT 
GGGTGCAAGG AGTCTGTGGA ATCACCCGTG TCACTACGGG TCTCTATATC CAAGCAACCA 
TCTGTATCGC CATTATCCCC AGCCCAGCCG GTCCCAGTAA TGAGGCCCAC TGGCCCCAGG 
GACAGTTGCC CAATAAATGT TCAAACTCCA AACCCGGAGC GAGGAGTGAA GGGTGCCCTG 
GACTCTGCCA CAGAACGAGG AATGAAGGAT GCTCTGGCCT CTGCCACTGA CGAGCAGAGC 
ACAACTACCT TGCCACCTGT GTTGCTCCCA GCTGCAGACT CACGAGGCTC AAAGACTGGA 
TCCTCTGTGC CTATTGGGTC ACCCGAGTCC CCTAGACTGC TGCTCAGATC TTCCCAGGGA 
GCCACAGCAT CCAGGGCAAC ACTTGGACTT CCGCCCCTAA GTCCTTCAAC ACCACCTGCT 
GGGCCACCTG TGGAGGATCT AGGCCCAGAA CGACCCACAG CTACAGGGTC TCCCGCCTTA 
TCACAGCTGA CAGGCTCGGC AGCACCGGGT ACCGGGAAGA AAGCCCCCCA CAAGCCTCTG 
CTGGTGGAGG GTCCTGGTGA GGATTCAAAT ACGGCAAAAA CGATGTTCAA GAGGAAGCAG 
AAGACCAATG CCACCAGAGA GTGCTTCCAC CGAAATGCTC AGTCCAAGAA GCCGGCTAGC 
AAACTGAAGT CTGCACCTTG TCCACTTAAC TTTCCTGTAC TCCCCAAGAG AGTTTGGGTC 
ACCTATAAAC CTCATCCCAG CCGGATCCAA GACTACAGCT ACCCCGAGGG AGTGAGTGGC 
CAGAACTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524645.1
CDS	217..1788
Translation	MGLAEVKKFFLGPLSPSCKMVQMMRQFLYDVLPEDSYKFATGKLHVSLTRVTDGENVVVSEYRSKEELIEALYCSCFVPVYCGFIPPTYRGERYIDGGFTSMQPCSFWTDSITISTFSSQQDICPRDCPTIFHDFRMFNFSFQFSLENITRMTHALFPPDLVILQEYYYRGYNDAVSYLRRLNAAYLDSPSKRVIFPRVEVYCQIEVALGHEPPPPSLQNLPALRRSPADSSQTHAQGSPKKDRKDSHSSAAPSVQTPESGCKESVESPVSLRVSISKQPSVSPLSPAQPVPVMRPTGPRDSCPINVQTPNPERGVKGALDSATERGMKDALASATDEQSTTTLPPVLLPAADSRGSKTGSSVPIGSPESPRLLLRSSQGATASRATLGLPPLSPSTPPAGPPVEDLGPERPTATGSPALSQLTGSAAPGTGKKAPHKPLLVEGPGEDSNTAKTMFKRKQKTNATRECFHRNAQSKKPASKLKSAPCPLNFPVLPKRVWVTYKPHPSRIQDYSYPEGVSGQNS

Target ORF information:

RefSeq Version	XM_006524582.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 1 (Pnpla1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524582.4

ORF Insert Sequence:

ATGGGCCTGG CGGAGGTGAA GAAGTTCTTC CTGGGACCTC TGTCTCCGTC CTGCAAGATG 
GTACAGATGA TGCGACAGTT TTTGTACGAT GTGCTACCGG AGGACTCCTA CAAGTTCGCC 
ACCGGGAAGC TGCATGTGAG CCTCACCCGA GTCACGGACG GAGAGAACGT CGTGGTTTCC 
GAGTACCGAT CCAAGGAAGA GCTCATCGAG GCCCTGTATT GCAGCTGCTT TGTTCCTGTT 
TACTGTGGCT TCATCCCCCC AACGTATCGG GGAGAGAGAT ACATCGACGG TGGCTTCACA 
AGCATGCAGC CCTGTTCCTT CTGGACAGAC TCCATCACCA TCTCCACCTT CAGCAGCCAG 
CAGGACATCT GTCCGAGAGA CTGCCCCACC ATCTTCCATG ACTTCCGAAT GTTCAACTTC 
TCCTTCCAGT TCTCCCTGGA GAATATCACC CGCATGACAC ATGCGCTGTT TCCCCCGGAC 
CTGGTGATTC TGCAGGAATA TTACTATCGG GGATACAATG ATGCTGTCTC ATACCTGCGG 
AGACTGAATG CAGCGTACCT TGACTCTCCC AGCAAGAGAG TGATTTTCCC GAGGGTTGAA 
GTATACTGCC AGATAGAGGT CGCCCTTGGC CATGAGCCCC CACCTCCGAG TCTGCAGAAC 
CTGCCAGCCC TGAGGAGAAG CCCAGCAGAC TCCTCACAAA CCCATGCACA GGGGTCTCCC 
AAAAAGGACA GAAAGGACAG CCATTCCTCA GCCGCCCCCT CAGTGCAGAC ACCTGAATCT 
GGGTGCAAGG AGTCTGTGGA ATCACCCGTG TCACTACGGG TCTCTATATC CAAGCAACCA 
TCTGTATCGC CATTATCCCC AGCCCAGCCG GTCCCAGTAA TGAGGCCCAC TGGCCCCAGG 
GACAGTTGCC CAATAAATGT TCAAACTCCA AACCCGGAGC GAGGAGTGAA GGGTGCCCTG 
GACTCTGCCA CAGAACGAGG AATGAAGGAT GCTCTGGCCT CTGCCACTGA CGAGCAGAGC 
ACAACTACCT TGCCACCTGT GTTGCTCCCA GCTGCAGACT CACGAGGCTC AAAGACTGGA 
TCCTCTGTGC CTATTGGGTC ACCCGAGTCC CCTAGACTGC TGCTCAGATC TTCCCAGGGA 
GCCACAGCAT CCAGGGCAAC ACTTGGACTT CCGCCCCTAA GTCCTTCAAC ACCACCTGCT 
GGGCCACCTG TGGAGGATCT AGGCCCAGAA CGACCCACAG CTACAGGGTC TCCCGCCTTA 
TCACAGCTGA CAGGCTCGGC AGCACCGGGT ACCGGGAAGA AAGCCCCCCA CAAGCCTCTG 
CTGGTGGAGG GTCCTGGTGA GGATTCAAAT ACGGCAAAAA CGATGTTCAA GAGGAAGCAG 
AAGACCAATG CCACCAGAGA GTGCTTCCAC CGAAATGCTC AGTCCAAGAA GCCGGCTAGC 
AAACTGAAGT CTGCACCTTG TCCACTTAAC TTTCCTGTAC TCCCCAAGAG AGTTTGGGTC 
ACCTATAAAC CTCATCCCAG CCGGATCCAA GACTACAGCT ACCCCGAGGG AGTGAGTGGC 
CAGAACTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnpla1 ( XM_006524581.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524644.1 Mus musculus patatin-like phospholipase domain containing 1 (Pnpla1), transcript variant X1, mRNA.

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CloneID	OMu53806
Clone ID Related Accession (Same CDS sequence)	XM_006524581.3 , XM_006524582.4 , XM_006524582.3 , XM_006524581.4
Accession Version	XM_006524581.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	patatin-like phospholipase domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524581.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCTGG CGGAGGTGAA GAAGTTCTTC CTGGGACCTC TGTCTCCGTC CTGCAAGATG 
GTACAGATGA TGCGACAGTT TTTGTACGAT GTGCTACCGG AGGACTCCTA CAAGTTCGCC 
ACCGGGAAGC TGCATGTGAG CCTCACCCGA GTCACGGACG GAGAGAACGT CGTGGTTTCC 
GAGTACCGAT CCAAGGAAGA GCTCATCGAG GCCCTGTATT GCAGCTGCTT TGTTCCTGTT 
TACTGTGGCT TCATCCCCCC AACGTATCGG GGAGAGAGAT ACATCGACGG TGGCTTCACA 
AGCATGCAGC CCTGTTCCTT CTGGACAGAC TCCATCACCA TCTCCACCTT CAGCAGCCAG 
CAGGACATCT GTCCGAGAGA CTGCCCCACC ATCTTCCATG ACTTCCGAAT GTTCAACTTC 
TCCTTCCAGT TCTCCCTGGA GAATATCACC CGCATGACAC ATGCGCTGTT TCCCCCGGAC 
CTGGTGATTC TGCAGGAATA TTACTATCGG GGATACAATG ATGCTGTCTC ATACCTGCGG 
AGACTGAATG CAGCGTACCT TGACTCTCCC AGCAAGAGAG TGATTTTCCC GAGGGTTGAA 
GTATACTGCC AGATAGAGGT CGCCCTTGGC CATGAGCCCC CACCTCCGAG TCTGCAGAAC 
CTGCCAGCCC TGAGGAGAAG CCCAGCAGAC TCCTCACAAA CCCATGCACA GGGGTCTCCC 
AAAAAGGACA GAAAGGACAG CCATTCCTCA GCCGCCCCCT CAGTGCAGAC ACCTGAATCT 
GGGTGCAAGG AGTCTGTGGA ATCACCCGTG TCACTACGGG TCTCTATATC CAAGCAACCA 
TCTGTATCGC CATTATCCCC AGCCCAGCCG GTCCCAGTAA TGAGGCCCAC TGGCCCCAGG 
GACAGTTGCC CAATAAATGT TCAAACTCCA AACCCGGAGC GAGGAGTGAA GGGTGCCCTG 
GACTCTGCCA CAGAACGAGG AATGAAGGAT GCTCTGGCCT CTGCCACTGA CGAGCAGAGC 
ACAACTACCT TGCCACCTGT GTTGCTCCCA GCTGCAGACT CACGAGGCTC AAAGACTGGA 
TCCTCTGTGC CTATTGGGTC ACCCGAGTCC CCTAGACTGC TGCTCAGATC TTCCCAGGGA 
GCCACAGCAT CCAGGGCAAC ACTTGGACTT CCGCCCCTAA GTCCTTCAAC ACCACCTGCT 
GGGCCACCTG TGGAGGATCT AGGCCCAGAA CGACCCACAG CTACAGGGTC TCCCGCCTTA 
TCACAGCTGA CAGGCTCGGC AGCACCGGGT ACCGGGAAGA AAGCCCCCCA CAAGCCTCTG 
CTGGTGGAGG GTCCTGGTGA GGATTCAAAT ACGGCAAAAA CGATGTTCAA GAGGAAGCAG 
AAGACCAATG CCACCAGAGA GTGCTTCCAC CGAAATGCTC AGTCCAAGAA GCCGGCTAGC 
AAACTGAAGT CTGCACCTTG TCCACTTAAC TTTCCTGTAC TCCCCAAGAG AGTTTGGGTC 
ACCTATAAAC CTCATCCCAG CCGGATCCAA GACTACAGCT ACCCCGAGGG AGTGAGTGGC 
CAGAACTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524644.1
CDS	252..1823
Translation	MGLAEVKKFFLGPLSPSCKMVQMMRQFLYDVLPEDSYKFATGKLHVSLTRVTDGENVVVSEYRSKEELIEALYCSCFVPVYCGFIPPTYRGERYIDGGFTSMQPCSFWTDSITISTFSSQQDICPRDCPTIFHDFRMFNFSFQFSLENITRMTHALFPPDLVILQEYYYRGYNDAVSYLRRLNAAYLDSPSKRVIFPRVEVYCQIEVALGHEPPPPSLQNLPALRRSPADSSQTHAQGSPKKDRKDSHSSAAPSVQTPESGCKESVESPVSLRVSISKQPSVSPLSPAQPVPVMRPTGPRDSCPINVQTPNPERGVKGALDSATERGMKDALASATDEQSTTTLPPVLLPAADSRGSKTGSSVPIGSPESPRLLLRSSQGATASRATLGLPPLSPSTPPAGPPVEDLGPERPTATGSPALSQLTGSAAPGTGKKAPHKPLLVEGPGEDSNTAKTMFKRKQKTNATRECFHRNAQSKKPASKLKSAPCPLNFPVLPKRVWVTYKPHPSRIQDYSYPEGVSGQNS

Target ORF information:

RefSeq Version	XM_006524581.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus patatin-like phospholipase domain containing 1 (Pnpla1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524581.4

ORF Insert Sequence:

ATGGGCCTGG CGGAGGTGAA GAAGTTCTTC CTGGGACCTC TGTCTCCGTC CTGCAAGATG 
GTACAGATGA TGCGACAGTT TTTGTACGAT GTGCTACCGG AGGACTCCTA CAAGTTCGCC 
ACCGGGAAGC TGCATGTGAG CCTCACCCGA GTCACGGACG GAGAGAACGT CGTGGTTTCC 
GAGTACCGAT CCAAGGAAGA GCTCATCGAG GCCCTGTATT GCAGCTGCTT TGTTCCTGTT 
TACTGTGGCT TCATCCCCCC AACGTATCGG GGAGAGAGAT ACATCGACGG TGGCTTCACA 
AGCATGCAGC CCTGTTCCTT CTGGACAGAC TCCATCACCA TCTCCACCTT CAGCAGCCAG 
CAGGACATCT GTCCGAGAGA CTGCCCCACC ATCTTCCATG ACTTCCGAAT GTTCAACTTC 
TCCTTCCAGT TCTCCCTGGA GAATATCACC CGCATGACAC ATGCGCTGTT TCCCCCGGAC 
CTGGTGATTC TGCAGGAATA TTACTATCGG GGATACAATG ATGCTGTCTC ATACCTGCGG 
AGACTGAATG CAGCGTACCT TGACTCTCCC AGCAAGAGAG TGATTTTCCC GAGGGTTGAA 
GTATACTGCC AGATAGAGGT CGCCCTTGGC CATGAGCCCC CACCTCCGAG TCTGCAGAAC 
CTGCCAGCCC TGAGGAGAAG CCCAGCAGAC TCCTCACAAA CCCATGCACA GGGGTCTCCC 
AAAAAGGACA GAAAGGACAG CCATTCCTCA GCCGCCCCCT CAGTGCAGAC ACCTGAATCT 
GGGTGCAAGG AGTCTGTGGA ATCACCCGTG TCACTACGGG TCTCTATATC CAAGCAACCA 
TCTGTATCGC CATTATCCCC AGCCCAGCCG GTCCCAGTAA TGAGGCCCAC TGGCCCCAGG 
GACAGTTGCC CAATAAATGT TCAAACTCCA AACCCGGAGC GAGGAGTGAA GGGTGCCCTG 
GACTCTGCCA CAGAACGAGG AATGAAGGAT GCTCTGGCCT CTGCCACTGA CGAGCAGAGC 
ACAACTACCT TGCCACCTGT GTTGCTCCCA GCTGCAGACT CACGAGGCTC AAAGACTGGA 
TCCTCTGTGC CTATTGGGTC ACCCGAGTCC CCTAGACTGC TGCTCAGATC TTCCCAGGGA 
GCCACAGCAT CCAGGGCAAC ACTTGGACTT CCGCCCCTAA GTCCTTCAAC ACCACCTGCT 
GGGCCACCTG TGGAGGATCT AGGCCCAGAA CGACCCACAG CTACAGGGTC TCCCGCCTTA 
TCACAGCTGA CAGGCTCGGC AGCACCGGGT ACCGGGAAGA AAGCCCCCCA CAAGCCTCTG 
CTGGTGGAGG GTCCTGGTGA GGATTCAAAT ACGGCAAAAA CGATGTTCAA GAGGAAGCAG 
AAGACCAATG CCACCAGAGA GTGCTTCCAC CGAAATGCTC AGTCCAAGAA GCCGGCTAGC 
AAACTGAAGT CTGCACCTTG TCCACTTAAC TTTCCTGTAC TCCCCAAGAG AGTTTGGGTC 
ACCTATAAAC CTCATCCCAG CCGGATCCAA GACTACAGCT ACCCCGAGGG AGTGAGTGGC 
CAGAACTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PNPLA1 defects in patients with autosomal recessive congenital ichthyosis and KO mice sustain PNPLA1 irreplaceable function in epidermal omega-O-acylceramide synthesis and skin permeability barrier.
Human molecular genetics26(10)1787-1800(2017 05)
Pichery M,Huchenq A,Sandhoff R,Severino-Freire M,Zaafouri S,Op?lka L,Levade T,Soldan V,Bertrand-Michel J,Lhuillier E,Serre G,Maruani A,Mazereeuw-Hautier J,Jonca N

PNPLA1 has a crucial role in skin barrier function by directing acylceramide biosynthesis.
Nature communications814609(2017 Mar)
Hirabayashi T,Anjo T,Kaneko A,Senoo Y,Shibata A,Takama H,Yokoyama K,Nishito Y,Ono T,Taya C,Muramatsu K,Fukami K,Mu?oz-Garcia A,Brash AR,Ikeda K,Arita M,Akiyama M,Murakami M

PNPLA1 Deficiency in Mice and Humans?Leads to a Defect in the Synthesis?of Omega-O-Acylceramides.
The Journal of investigative dermatology137(2)394-402(2017 Feb)
Grond S,Eichmann TO,Dubrac S,Kolb D,Schmuth M,Fischer J,Crumrine D,Elias PM,Haemmerle G,Zechner R,Lass A,Radner FP

Identification mouse patatin-like phospholipase domain containing protein 1 as a skin-specific and membrane-associated protein.
Gene591(2)344-50(2016 Oct)
Chang PA,Han LP,Sun LX,Huang FF

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Pnpla1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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