Wee2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wee2
Entrez Gene ID	381759
Full Name	WEE1 homolog 2 (S. pombe)
Synonyms	Gm1065,Wee1B
General protein information	Preferred Names WEE1 homolog 2 (S. pombe) Names wee1-like protein kinase 2 mWee1B wee1-like protein kinase 1B wee1B kinase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 B1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006506327.3	XP_006506390.1	wee1-like protein kinase 2 isoform X2
XM_006506326.4	XP_006506389.1	wee1-like protein kinase 2 isoform X1
XM_006506326.3	XP_006506389.1	wee1-like protein kinase 2 isoform X1
XM_006506327.4	XP_006506390.1	wee1-like protein kinase 2 isoform X2
NM_201370.2	NP_958758.2	wee1-like protein kinase 2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	WEE2	XP_002687121.1
Rattus norvegicus (Norway rat)	Wee2	XP_006236426.1
Homo sapiens (human)	WEE2	NP_001099028.1
	WEE2	NP_001099016.1
Gallus gallus (chicken)	WEE2	XP_004937931.1
Canis lupus familiaris (dog)	WEE2	XP_532748.3
Mus musculus (house mouse)	Wee2	NP_958758.2
Danio rerio (zebrafish)	wee2	NP_001038367.1
Xenopus tropicalis (tropical clawed frog)	wee2	XP_004912526.1
Pan troglodytes (chimpanzee)	WEE2	XP_519504.3

Gene Ontology
Bibliography

Related articles in PubMed

CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins.
Yu C, Zhang YL, Pan WW, Li XM, Wang ZW, Ge ZJ, Zhou JJ, Cang Y, Tong C, Sun QY, Fan HY
Science (New York, N.Y.)342(6165)1518-21(2013 Dec)

Ser 15 of WEE1B is a potential PKA phosphorylation target in G2/M transition in one-cell stage mouse embryos.
Liu C, Liu Y, Liu Y, Wu D, Luan Z, Wang E, Yu B
Molecular medicine reports7(6)1929-37(2013 Jun)

MARF1 regulates essential oogenic processes in mice.
Su YQ, Sugiura K, Sun F, Pendola JK, Cox GA, Handel MA, Schimenti JC, Eppig JJ
Science (New York, N.Y.)335(6075)1496-9(2012 Mar)

Protein tyrosine kinase Wee1B is essential for metaphase II exit in mouse oocytes.
Oh JS, Susor A, Conti M
Science (New York, N.Y.)332(6028)462-5(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

WEE1B acts as a direct downstream substrate of protein kinase A (PKA) and that Ser 15 of WEE1B is a potential PKA phosphorylation target in the G2/M transition of mouse embryos.
Title: Ser 15 of WEE1B is a potential PKA phosphorylation target in G2/M transition in one-cell stage mouse embryos.

findings demonstrate that exit from metaphase requires not only a proteolytic degradation of cyclin B but also the inhibitory phosphorylation of Cdc2 by Wee1B
Title: Protein tyrosine kinase Wee1B is essential for metaphase II exit in mouse oocytes.

Wee1B is a key maturation-promoting-factor inhibitory kinase in mouse oocytes, functions downstream of PKA, and is required for maintaining meiotic arrest.
Title: Wee1B is an oocyte-specific kinase involved in the control of meiotic arrest in the mouse.

The following Wee2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wee2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18090	NM_201370.2 Latest version!	Mus musculus WEE1 homolog 2 (S. pombe) (Wee2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu37405	XM_006506327.4 Latest version!	Mus musculus WEE1 homolog 2 (S. pombe) (Wee2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu18090	XM_006506326.4 Latest version!	Mus musculus WEE1 homolog 2 (S. pombe) (Wee2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu18090	XM_006506326.3	Mus musculus WEE1 homolog 2 (S. pombe) (Wee2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu37405	XM_006506327.3	Mus musculus WEE1 homolog 2 (S. pombe) (Wee2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wee2 ( NM_201370.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_958758.2 Mus musculus WEE1 homolog 2 (S. pombe) (Wee2), mRNA.

Price & Availability↑

CloneID	OMu18090
Clone ID Related Accession (Same CDS sequence)	XM_006506326.4 , XM_006506326.3 , NM_201370.2
Accession Version	NM_201370.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	wee1-like protein kinase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY725757.1, AK139573.1 and CJ200934.1. On Apr 2, 2008 this sequence version replaced NM_201370.1. ##Evidence-Data-START## Transcript exon combination :: AK139573.1, BC080784.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGACA CAGAGACTGA CCAGGGACTG AACAAGAAAT TAAGCTTCTC CTTTTGTGAA 
GAAGACACTG AGAGTGAAGG GCAGATGACT GCACAAGACA TCGGAGGGGC CCAGAGCCAA 
AAGCCTGGGA GGGAAGAAAG TGAGGAGTTG GAGGGTCCCG TGCCTCCTAC CAGAGATGAA 
CTTCATACTT CTCTGAGCAG AGACAAGGAA AGTCCAGGTC CTGATCTTTG GAAATCAGTT 
TCAGGCCCTC CCAAATGCCC AGAGACACCA GTATTACACA GCAAGAGCAA GCTGCCAGTC 
CCTACCAACT TCTCCACTCC CAAAAATTCG CTGGGTCAGC CAGAGATTTC TCTGCTAGAG 
AAGCTCTCCT CCCGAAGCTC TAGACATATG AGGCTCACAC CTGCTTCCCT TATGGATGAG 
AAGACCTCTT TGTCTCTGGT CAATATCAAC CCATTCACTC CGGAGACCTA CAGAAAATTA 
CTCCTTCAAT CAAAGGGCAA GAGGAAGACC AGAGACTATC TTGAGGAAAC TGGAGAAGAG 
GAAAGCAAAT CAGTACAGTG GCTGCCTGCT AAGAGAAGTA TTCTACAAGA AACTAATATG 
GCTTCCCGCT ATGAAAAAGA ATTCTTCGAG ATAGAAAAAA TCGGGGTTGG AGAATTTGGG 
ACAGTCTACA AGTGCATTAA GAGGCTGGAT GGATGCATTT ATGCAATAAA GCGCTCTGCA 
AAGTCCTTTT CAGGATTATC AAATGAATTG GATTTGCATG AAGTTTATGC TCATGCAGTG 
CTTGGCCATC ATCCCCATGT GGTACGCTAC TACTCATCAT GGATAGAAGA TGATCATGTG 
GTCATTCAAA ATGAATACTG TAATGGTGGG AGCCTGCAAG CTGCTATATC TGAAAACACT 
GCGTCCAATA ATCACTTCCA AGAGCCCAAA CTCAAAGACA TCCTTCTGCA GATTTCCTTG 
GGACTTAAGT ACATCCACAA TTCTGGCATG GTACACCTGG ACATCAAACC CAGTAACATC 
TTCATTTGTC ATAAGATGCA GTGTGACTCT CCTGTAGGCC CAGAAGAGGC TGAAAGTGAA 
GCTGACTGGT TTCTCAATGC CAGTGTGATG TATAAAATTG GTGACCTGGG CCACGCGACA 
TCAATCAGCA AACCTAAAGT GGAAGAAGGA GATACTCGTT TCCTGGCTAA TGAGATCTTG 
CAAGAGAATT ACCAACACCT CCCCAAAGCA GACATATTTG CCTTAGGATT AACAATCGCA 
GTGGCTGCAG GAGCAGAGTC TTTACCCATC AATGGTGACA TGTGGCATCA TATCCGCAAA 
GGGAACTTTC CAGAGATTTC CCAAGAGCTC TCGGATGACT TTTATGGTCT ACTCAAGAAC 
ATGATCCACC CAGCTCCAAA GGAGAGACCA TCTGCAGCAG CTCTGGCCAG AAGTCGAATT 
CTCTGGCCTT TCTTGGAGAA GACTGATGAA CTCCAGAAAC AACTGAATTT GGAGAAATCC 
AAGACAGCCA CACTGAAAAG GGAACTGAAA AAAGCTCGGC ATATCCAGAC CCCTCAGAGA 
GAAGTCCACC ATTGTTATTA TCAGATCTGC AAAGGCTCAA GACACCTCCT GGTGGGAGGA 
AGGAGGAAGG CGCCCTCAAG CTTCACCCGT GGGACATCCT CCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_958758.2
CDS	367..2034
Translation	MADTETDQGLNKKLSFSFCEEDTESEGQMTAQDIGGAQSQKPGREESEELEGPVPPTRDELHTSLSRDKESPGPDLWKSVSGPPKCPETPVLHSKSKLPVPTNFSTPKNSLGQPEISLLEKLSSRSSRHMRLTPASLMDEKTSLSLVNINPFTPETYRKLLLQSKGKRKTRDYLEETGEEESKSVQWLPAKRSILQETNMASRYEKEFFEIEKIGVGEFGTVYKCIKRLDGCIYAIKRSAKSFSGLSNELDLHEVYAHAVLGHHPHVVRYYSSWIEDDHVVIQNEYCNGGSLQAAISENTASNNHFQEPKLKDILLQISLGLKYIHNSGMVHLDIKPSNIFICHKMQCDSPVGPEEAESEADWFLNASVMYKIGDLGHATSISKPKVEEGDTRFLANEILQENYQHLPKADIFALGLTIAVAAGAESLPINGDMWHHIRKGNFPEISQELSDDFYGLLKNMIHPAPKERPSAAALARSRILWPFLEKTDELQKQLNLEKSKTATLKRELKKARHIQTPQREVHHCYYQICKGSRHLLVGGRRKAPSSFTRGTSSV

Target ORF information:

RefSeq Version	NM_201370.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus WEE1 homolog 2 (S. pombe) (Wee2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201370.2

ORF Insert Sequence:

ATGGCCGACA CAGAGACTGA CCAGGGACTG AACAAGAAAT TAAGCTTCTC CTTTTGTGAA 
GAAGACACTG AGAGTGAAGG GCAGATGACT GCACAAGACA TCGGAGGGGC CCAGAGCCAA 
AAGCCTGGGA GGGAAGAAAG TGAGGAGTTG GAGGGTCCCG TGCCTCCTAC CAGAGATGAA 
CTTCATACTT CTCTGAGCAG AGACAAGGAA AGTCCAGGTC CTGATCTTTG GAAATCAGTT 
TCAGGCCCTC CCAAATGCCC AGAGACACCA GTATTACACA GCAAGAGCAA GCTGCCAGTC 
CCTACCAACT TCTCCACTCC CAAAAATTCG CTGGGTCAGC CAGAGATTTC TCTGCTAGAG 
AAGCTCTCCT CCCGAAGCTC TAGACATATG AGGCTCACAC CTGCTTCCCT TATGGATGAG 
AAGACCTCTT TGTCTCTGGT CAATATCAAC CCATTCACTC CGGAGACCTA CAGAAAATTA 
CTCCTTCAAT CAAAGGGCAA GAGGAAGACC AGAGACTATC TTGAGGAAAC TGGAGAAGAG 
GAAAGCAAAT CAGTACAGTG GCTGCCTGCT AAGAGAAGTA TTCTACAAGA AACTAATATG 
GCTTCCCGCT ATGAAAAAGA ATTCTTCGAG ATAGAAAAAA TCGGGGTTGG AGAATTTGGG 
ACAGTCTACA AGTGCATTAA GAGGCTGGAT GGATGCATTT ATGCAATAAA GCGCTCTGCA 
AAGTCCTTTT CAGGATTATC AAATGAATTG GATTTGCATG AAGTTTATGC TCATGCAGTG 
CTTGGCCATC ATCCCCATGT GGTACGCTAC TACTCATCAT GGATAGAAGA TGATCATGTG 
GTCATTCAAA ATGAATACTG TAATGGTGGG AGCCTGCAAG CTGCTATATC TGAAAACACT 
GCGTCCAATA ATCACTTCCA AGAGCCCAAA CTCAAAGACA TCCTTCTGCA GATTTCCTTG 
GGACTTAAGT ACATCCACAA TTCTGGCATG GTACACCTGG ACATCAAACC CAGTAACATC 
TTCATTTGTC ATAAGATGCA GTGTGACTCT CCTGTAGGCC CAGAAGAGGC TGAAAGTGAA 
GCTGACTGGT TTCTCAATGC CAGTGTGATG TATAAAATTG GTGACCTGGG CCACGCGACA 
TCAATCAGCA AACCTAAAGT GGAAGAAGGA GATACTCGTT TCCTGGCTAA TGAGATCTTG 
CAAGAGAATT ACCAACACCT CCCCAAAGCA GACATATTTG CCTTAGGATT AACAATCGCA 
GTGGCTGCAG GAGCAGAGTC TTTACCCATC AATGGTGACA TGTGGCATCA TATCCGCAAA 
GGGAACTTTC CAGAGATTTC CCAAGAGCTC TCGGATGACT TTTATGGTCT ACTCAAGAAC 
ATGATCCACC CAGCTCCAAA GGAGAGACCA TCTGCAGCAG CTCTGGCCAG AAGTCGAATT 
CTCTGGCCTT TCTTGGAGAA GACTGATGAA CTCCAGAAAC AACTGAATTT GGAGAAATCC 
AAGACAGCCA CACTGAAAAG GGAACTGAAA AAAGCTCGGC ATATCCAGAC CCCTCAGAGA 
GAAGTCCACC ATTGTTATTA TCAGATCTGC AAAGGCTCAA GACACCTCCT GGTGGGAGGA 
AGGAGGAAGG CGCCCTCAAG CTTCACCCGT GGGACATCCT CCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wee2 ( XM_006506326.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506389.1 Mus musculus WEE1 homolog 2 (S. pombe) (Wee2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu18090
Clone ID Related Accession (Same CDS sequence)	XM_006506326.4 , XM_006506326.3 , NM_201370.2
Accession Version	XM_006506326.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	wee1-like protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506326.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGACA CAGAGACTGA CCAGGGACTG AACAAGAAAT TAAGCTTCTC CTTTTGTGAA 
GAAGACACTG AGAGTGAAGG GCAGATGACT GCACAAGACA TCGGAGGGGC CCAGAGCCAA 
AAGCCTGGGA GGGAAGAAAG TGAGGAGTTG GAGGGTCCCG TGCCTCCTAC CAGAGATGAA 
CTTCATACTT CTCTGAGCAG AGACAAGGAA AGTCCAGGTC CTGATCTTTG GAAATCAGTT 
TCAGGCCCTC CCAAATGCCC AGAGACACCA GTATTACACA GCAAGAGCAA GCTGCCAGTC 
CCTACCAACT TCTCCACTCC CAAAAATTCG CTGGGTCAGC CAGAGATTTC TCTGCTAGAG 
AAGCTCTCCT CCCGAAGCTC TAGACATATG AGGCTCACAC CTGCTTCCCT TATGGATGAG 
AAGACCTCTT TGTCTCTGGT CAATATCAAC CCATTCACTC CGGAGACCTA CAGAAAATTA 
CTCCTTCAAT CAAAGGGCAA GAGGAAGACC AGAGACTATC TTGAGGAAAC TGGAGAAGAG 
GAAAGCAAAT CAGTACAGTG GCTGCCTGCT AAGAGAAGTA TTCTACAAGA AACTAATATG 
GCTTCCCGCT ATGAAAAAGA ATTCTTCGAG ATAGAAAAAA TCGGGGTTGG AGAATTTGGG 
ACAGTCTACA AGTGCATTAA GAGGCTGGAT GGATGCATTT ATGCAATAAA GCGCTCTGCA 
AAGTCCTTTT CAGGATTATC AAATGAATTG GATTTGCATG AAGTTTATGC TCATGCAGTG 
CTTGGCCATC ATCCCCATGT GGTACGCTAC TACTCATCAT GGATAGAAGA TGATCATGTG 
GTCATTCAAA ATGAATACTG TAATGGTGGG AGCCTGCAAG CTGCTATATC TGAAAACACT 
GCGTCCAATA ATCACTTCCA AGAGCCCAAA CTCAAAGACA TCCTTCTGCA GATTTCCTTG 
GGACTTAAGT ACATCCACAA TTCTGGCATG GTACACCTGG ACATCAAACC CAGTAACATC 
TTCATTTGTC ATAAGATGCA GTGTGACTCT CCTGTAGGCC CAGAAGAGGC TGAAAGTGAA 
GCTGACTGGT TTCTCAATGC CAGTGTGATG TATAAAATTG GTGACCTGGG CCACGCGACA 
TCAATCAGCA AACCTAAAGT GGAAGAAGGA GATACTCGTT TCCTGGCTAA TGAGATCTTG 
CAAGAGAATT ACCAACACCT CCCCAAAGCA GACATATTTG CCTTAGGATT AACAATCGCA 
GTGGCTGCAG GAGCAGAGTC TTTACCCATC AATGGTGACA TGTGGCATCA TATCCGCAAA 
GGGAACTTTC CAGAGATTTC CCAAGAGCTC TCGGATGACT TTTATGGTCT ACTCAAGAAC 
ATGATCCACC CAGCTCCAAA GGAGAGACCA TCTGCAGCAG CTCTGGCCAG AAGTCGAATT 
CTCTGGCCTT TCTTGGAGAA GACTGATGAA CTCCAGAAAC AACTGAATTT GGAGAAATCC 
AAGACAGCCA CACTGAAAAG GGAACTGAAA AAAGCTCGGC ATATCCAGAC CCCTCAGAGA 
GAAGTCCACC ATTGTTATTA TCAGATCTGC AAAGGCTCAA GACACCTCCT GGTGGGAGGA 
AGGAGGAAGG CGCCCTCAAG CTTCACCCGT GGGACATCCT CCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506389.1
CDS	392..2059
Translation	MADTETDQGLNKKLSFSFCEEDTESEGQMTAQDIGGAQSQKPGREESEELEGPVPPTRDELHTSLSRDKESPGPDLWKSVSGPPKCPETPVLHSKSKLPVPTNFSTPKNSLGQPEISLLEKLSSRSSRHMRLTPASLMDEKTSLSLVNINPFTPETYRKLLLQSKGKRKTRDYLEETGEEESKSVQWLPAKRSILQETNMASRYEKEFFEIEKIGVGEFGTVYKCIKRLDGCIYAIKRSAKSFSGLSNELDLHEVYAHAVLGHHPHVVRYYSSWIEDDHVVIQNEYCNGGSLQAAISENTASNNHFQEPKLKDILLQISLGLKYIHNSGMVHLDIKPSNIFICHKMQCDSPVGPEEAESEADWFLNASVMYKIGDLGHATSISKPKVEEGDTRFLANEILQENYQHLPKADIFALGLTIAVAAGAESLPINGDMWHHIRKGNFPEISQELSDDFYGLLKNMIHPAPKERPSAAALARSRILWPFLEKTDELQKQLNLEKSKTATLKRELKKARHIQTPQREVHHCYYQICKGSRHLLVGGRRKAPSSFTRGTSSV

Target ORF information:

RefSeq Version	XM_006506326.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus WEE1 homolog 2 (S. pombe) (Wee2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506326.3

ORF Insert Sequence:

ATGGCCGACA CAGAGACTGA CCAGGGACTG AACAAGAAAT TAAGCTTCTC CTTTTGTGAA 
GAAGACACTG AGAGTGAAGG GCAGATGACT GCACAAGACA TCGGAGGGGC CCAGAGCCAA 
AAGCCTGGGA GGGAAGAAAG TGAGGAGTTG GAGGGTCCCG TGCCTCCTAC CAGAGATGAA 
CTTCATACTT CTCTGAGCAG AGACAAGGAA AGTCCAGGTC CTGATCTTTG GAAATCAGTT 
TCAGGCCCTC CCAAATGCCC AGAGACACCA GTATTACACA GCAAGAGCAA GCTGCCAGTC 
CCTACCAACT TCTCCACTCC CAAAAATTCG CTGGGTCAGC CAGAGATTTC TCTGCTAGAG 
AAGCTCTCCT CCCGAAGCTC TAGACATATG AGGCTCACAC CTGCTTCCCT TATGGATGAG 
AAGACCTCTT TGTCTCTGGT CAATATCAAC CCATTCACTC CGGAGACCTA CAGAAAATTA 
CTCCTTCAAT CAAAGGGCAA GAGGAAGACC AGAGACTATC TTGAGGAAAC TGGAGAAGAG 
GAAAGCAAAT CAGTACAGTG GCTGCCTGCT AAGAGAAGTA TTCTACAAGA AACTAATATG 
GCTTCCCGCT ATGAAAAAGA ATTCTTCGAG ATAGAAAAAA TCGGGGTTGG AGAATTTGGG 
ACAGTCTACA AGTGCATTAA GAGGCTGGAT GGATGCATTT ATGCAATAAA GCGCTCTGCA 
AAGTCCTTTT CAGGATTATC AAATGAATTG GATTTGCATG AAGTTTATGC TCATGCAGTG 
CTTGGCCATC ATCCCCATGT GGTACGCTAC TACTCATCAT GGATAGAAGA TGATCATGTG 
GTCATTCAAA ATGAATACTG TAATGGTGGG AGCCTGCAAG CTGCTATATC TGAAAACACT 
GCGTCCAATA ATCACTTCCA AGAGCCCAAA CTCAAAGACA TCCTTCTGCA GATTTCCTTG 
GGACTTAAGT ACATCCACAA TTCTGGCATG GTACACCTGG ACATCAAACC CAGTAACATC 
TTCATTTGTC ATAAGATGCA GTGTGACTCT CCTGTAGGCC CAGAAGAGGC TGAAAGTGAA 
GCTGACTGGT TTCTCAATGC CAGTGTGATG TATAAAATTG GTGACCTGGG CCACGCGACA 
TCAATCAGCA AACCTAAAGT GGAAGAAGGA GATACTCGTT TCCTGGCTAA TGAGATCTTG 
CAAGAGAATT ACCAACACCT CCCCAAAGCA GACATATTTG CCTTAGGATT AACAATCGCA 
GTGGCTGCAG GAGCAGAGTC TTTACCCATC AATGGTGACA TGTGGCATCA TATCCGCAAA 
GGGAACTTTC CAGAGATTTC CCAAGAGCTC TCGGATGACT TTTATGGTCT ACTCAAGAAC 
ATGATCCACC CAGCTCCAAA GGAGAGACCA TCTGCAGCAG CTCTGGCCAG AAGTCGAATT 
CTCTGGCCTT TCTTGGAGAA GACTGATGAA CTCCAGAAAC AACTGAATTT GGAGAAATCC 
AAGACAGCCA CACTGAAAAG GGAACTGAAA AAAGCTCGGC ATATCCAGAC CCCTCAGAGA 
GAAGTCCACC ATTGTTATTA TCAGATCTGC AAAGGCTCAA GACACCTCCT GGTGGGAGGA 
AGGAGGAAGG CGCCCTCAAG CTTCACCCGT GGGACATCCT CCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wee2 ( XM_006506327.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506390.1 Mus musculus WEE1 homolog 2 (S. pombe) (Wee2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu37405
Clone ID Related Accession (Same CDS sequence)	XM_006506327.3 , XM_006506327.4
Accession Version	XM_006506327.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	wee1-like protein kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506327.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAATT CGCTGGGTCA GCCAGAGATT TCTCTGCTAG AGAAGCTCTC CTCCCGAAGC 
TCTAGACATA TGAGGCTCAC ACCTGCTTCC CTTATGGATG AGAAGACCTC TTTGTCTCTG 
GTCAATATCA ACCCATTCAC TCCGGAGACC TACAGAAAAT TACTCCTTCA ATCAAAGGGC 
AAGAGGAAGA CCAGAGACTA TCTTGAGGAA ACTGGAGAAG AGGAAAGCAA ATCAGTACAG 
TGGCTGCCTG CTAAGAGAAG TATTCTACAA GAAACTAATA TGGCTTCCCG CTATGAAAAA 
GAATTCTTCG AGATAGAAAA AATCGGGGTT GGAGAATTTG GGACAGTCTA CAAGTGCATT 
AAGAGGCTGG ATGGATGCAT TTATGCAATA AAGCGCTCTG CAAAGTCCTT TTCAGGATTA 
TCAAATGAAT TGGATTTGCA TGAAGTTTAT GCTCATGCAG TGCTTGGCCA TCATCCCCAT 
GTGGTACGCT ACTACTCATC ATGGATAGAA GATGATCATG TGGTCATTCA AAATGAATAC 
TGTAATGGTG GGAGCCTGCA AGCTGCTATA TCTGAAAACA CTGCGTCCAA TAATCACTTC 
CAAGAGCCCA AACTCAAAGA CATCCTTCTG CAGATTTCCT TGGGACTTAA GTACATCCAC 
AATTCTGGCA TGGTACACCT GGACATCAAA CCCAGTAACA TCTTCATTTG TCATAAGATG 
CAGTGTGACT CTCCTGTAGG CCCAGAAGAG GCTGAAAGTG AAGCTGACTG GTTTCTCAAT 
GCCAGTGTGA TGTATAAAAT TGGTGACCTG GGCCACGCGA CATCAATCAG CAAACCTAAA 
GTGGAAGAAG GAGATACTCG TTTCCTGGCT AATGAGATCT TGCAAGAGAA TTACCAACAC 
CTCCCCAAAG CAGACATATT TGCCTTAGGA TTAACAATCG CAGTGGCTGC AGGAGCAGAG 
TCTTTACCCA TCAATGGTGA CATGTGGCAT CATATCCGCA AAGGGAACTT TCCAGAGATT 
TCCCAAGAGC TCTCGGATGA CTTTTATGGT CTACTCAAGA ACATGATCCA CCCAGCTCCA 
AAGGAGAGAC CATCTGCAGC AGCTCTGGCC AGAAGTCGAA TTCTCTGGCC TTTCTTGGAG 
AAGACTGATG AACTCCAGAA ACAACTGAAT TTGGAGAAAT CCAAGACAGC CACACTGAAA 
AGGGAACTGA AAAAAGCTCG GCATATCCAG ACCCCTCAGA GAGAAGTCCA CCATTGTTAT 
TATCAGATCT GCAAAGGCTC AAGACACCTC CTGGTGGGAG GAAGGAGGAA GGCGCCCTCA 
AGCTTCACCC GTGGGACATC CTCCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506390.1
CDS	342..1691
Translation	MQNSLGQPEISLLEKLSSRSSRHMRLTPASLMDEKTSLSLVNINPFTPETYRKLLLQSKGKRKTRDYLEETGEEESKSVQWLPAKRSILQETNMASRYEKEFFEIEKIGVGEFGTVYKCIKRLDGCIYAIKRSAKSFSGLSNELDLHEVYAHAVLGHHPHVVRYYSSWIEDDHVVIQNEYCNGGSLQAAISENTASNNHFQEPKLKDILLQISLGLKYIHNSGMVHLDIKPSNIFICHKMQCDSPVGPEEAESEADWFLNASVMYKIGDLGHATSISKPKVEEGDTRFLANEILQENYQHLPKADIFALGLTIAVAAGAESLPINGDMWHHIRKGNFPEISQELSDDFYGLLKNMIHPAPKERPSAAALARSRILWPFLEKTDELQKQLNLEKSKTATLKRELKKARHIQTPQREVHHCYYQICKGSRHLLVGGRRKAPSSFTRGTSSV

Target ORF information:

RefSeq Version	XM_006506327.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus WEE1 homolog 2 (S. pombe) (Wee2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506327.3

ORF Insert Sequence:

ATGCAGAATT CGCTGGGTCA GCCAGAGATT TCTCTGCTAG AGAAGCTCTC CTCCCGAAGC 
TCTAGACATA TGAGGCTCAC ACCTGCTTCC CTTATGGATG AGAAGACCTC TTTGTCTCTG 
GTCAATATCA ACCCATTCAC TCCGGAGACC TACAGAAAAT TACTCCTTCA ATCAAAGGGC 
AAGAGGAAGA CCAGAGACTA TCTTGAGGAA ACTGGAGAAG AGGAAAGCAA ATCAGTACAG 
TGGCTGCCTG CTAAGAGAAG TATTCTACAA GAAACTAATA TGGCTTCCCG CTATGAAAAA 
GAATTCTTCG AGATAGAAAA AATCGGGGTT GGAGAATTTG GGACAGTCTA CAAGTGCATT 
AAGAGGCTGG ATGGATGCAT TTATGCAATA AAGCGCTCTG CAAAGTCCTT TTCAGGATTA 
TCAAATGAAT TGGATTTGCA TGAAGTTTAT GCTCATGCAG TGCTTGGCCA TCATCCCCAT 
GTGGTACGCT ACTACTCATC ATGGATAGAA GATGATCATG TGGTCATTCA AAATGAATAC 
TGTAATGGTG GGAGCCTGCA AGCTGCTATA TCTGAAAACA CTGCGTCCAA TAATCACTTC 
CAAGAGCCCA AACTCAAAGA CATCCTTCTG CAGATTTCCT TGGGACTTAA GTACATCCAC 
AATTCTGGCA TGGTACACCT GGACATCAAA CCCAGTAACA TCTTCATTTG TCATAAGATG 
CAGTGTGACT CTCCTGTAGG CCCAGAAGAG GCTGAAAGTG AAGCTGACTG GTTTCTCAAT 
GCCAGTGTGA TGTATAAAAT TGGTGACCTG GGCCACGCGA CATCAATCAG CAAACCTAAA 
GTGGAAGAAG GAGATACTCG TTTCCTGGCT AATGAGATCT TGCAAGAGAA TTACCAACAC 
CTCCCCAAAG CAGACATATT TGCCTTAGGA TTAACAATCG CAGTGGCTGC AGGAGCAGAG 
TCTTTACCCA TCAATGGTGA CATGTGGCAT CATATCCGCA AAGGGAACTT TCCAGAGATT 
TCCCAAGAGC TCTCGGATGA CTTTTATGGT CTACTCAAGA ACATGATCCA CCCAGCTCCA 
AAGGAGAGAC CATCTGCAGC AGCTCTGGCC AGAAGTCGAA TTCTCTGGCC TTTCTTGGAG 
AAGACTGATG AACTCCAGAA ACAACTGAAT TTGGAGAAAT CCAAGACAGC CACACTGAAA 
AGGGAACTGA AAAAAGCTCG GCATATCCAG ACCCCTCAGA GAGAAGTCCA CCATTGTTAT 
TATCAGATCT GCAAAGGCTC AAGACACCTC CTGGTGGGAG GAAGGAGGAA GGCGCCCTCA 
AGCTTCACCC GTGGGACATC CTCCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wee2 ( XM_006506327.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506390.1 Mus musculus WEE1 homolog 2 (S. pombe) (Wee2), transcript variant X2, mRNA.

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CloneID	OMu37405
Clone ID Related Accession (Same CDS sequence)	XM_006506327.3 , XM_006506327.4
Accession Version	XM_006506327.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	wee1-like protein kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506327.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAATT CGCTGGGTCA GCCAGAGATT TCTCTGCTAG AGAAGCTCTC CTCCCGAAGC 
TCTAGACATA TGAGGCTCAC ACCTGCTTCC CTTATGGATG AGAAGACCTC TTTGTCTCTG 
GTCAATATCA ACCCATTCAC TCCGGAGACC TACAGAAAAT TACTCCTTCA ATCAAAGGGC 
AAGAGGAAGA CCAGAGACTA TCTTGAGGAA ACTGGAGAAG AGGAAAGCAA ATCAGTACAG 
TGGCTGCCTG CTAAGAGAAG TATTCTACAA GAAACTAATA TGGCTTCCCG CTATGAAAAA 
GAATTCTTCG AGATAGAAAA AATCGGGGTT GGAGAATTTG GGACAGTCTA CAAGTGCATT 
AAGAGGCTGG ATGGATGCAT TTATGCAATA AAGCGCTCTG CAAAGTCCTT TTCAGGATTA 
TCAAATGAAT TGGATTTGCA TGAAGTTTAT GCTCATGCAG TGCTTGGCCA TCATCCCCAT 
GTGGTACGCT ACTACTCATC ATGGATAGAA GATGATCATG TGGTCATTCA AAATGAATAC 
TGTAATGGTG GGAGCCTGCA AGCTGCTATA TCTGAAAACA CTGCGTCCAA TAATCACTTC 
CAAGAGCCCA AACTCAAAGA CATCCTTCTG CAGATTTCCT TGGGACTTAA GTACATCCAC 
AATTCTGGCA TGGTACACCT GGACATCAAA CCCAGTAACA TCTTCATTTG TCATAAGATG 
CAGTGTGACT CTCCTGTAGG CCCAGAAGAG GCTGAAAGTG AAGCTGACTG GTTTCTCAAT 
GCCAGTGTGA TGTATAAAAT TGGTGACCTG GGCCACGCGA CATCAATCAG CAAACCTAAA 
GTGGAAGAAG GAGATACTCG TTTCCTGGCT AATGAGATCT TGCAAGAGAA TTACCAACAC 
CTCCCCAAAG CAGACATATT TGCCTTAGGA TTAACAATCG CAGTGGCTGC AGGAGCAGAG 
TCTTTACCCA TCAATGGTGA CATGTGGCAT CATATCCGCA AAGGGAACTT TCCAGAGATT 
TCCCAAGAGC TCTCGGATGA CTTTTATGGT CTACTCAAGA ACATGATCCA CCCAGCTCCA 
AAGGAGAGAC CATCTGCAGC AGCTCTGGCC AGAAGTCGAA TTCTCTGGCC TTTCTTGGAG 
AAGACTGATG AACTCCAGAA ACAACTGAAT TTGGAGAAAT CCAAGACAGC CACACTGAAA 
AGGGAACTGA AAAAAGCTCG GCATATCCAG ACCCCTCAGA GAGAAGTCCA CCATTGTTAT 
TATCAGATCT GCAAAGGCTC AAGACACCTC CTGGTGGGAG GAAGGAGGAA GGCGCCCTCA 
AGCTTCACCC GTGGGACATC CTCCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506390.1
CDS	494..1843
Translation	MQNSLGQPEISLLEKLSSRSSRHMRLTPASLMDEKTSLSLVNINPFTPETYRKLLLQSKGKRKTRDYLEETGEEESKSVQWLPAKRSILQETNMASRYEKEFFEIEKIGVGEFGTVYKCIKRLDGCIYAIKRSAKSFSGLSNELDLHEVYAHAVLGHHPHVVRYYSSWIEDDHVVIQNEYCNGGSLQAAISENTASNNHFQEPKLKDILLQISLGLKYIHNSGMVHLDIKPSNIFICHKMQCDSPVGPEEAESEADWFLNASVMYKIGDLGHATSISKPKVEEGDTRFLANEILQENYQHLPKADIFALGLTIAVAAGAESLPINGDMWHHIRKGNFPEISQELSDDFYGLLKNMIHPAPKERPSAAALARSRILWPFLEKTDELQKQLNLEKSKTATLKRELKKARHIQTPQREVHHCYYQICKGSRHLLVGGRRKAPSSFTRGTSSV

Target ORF information:

RefSeq Version	XM_006506327.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus WEE1 homolog 2 (S. pombe) (Wee2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506327.4

ORF Insert Sequence:

ATGCAGAATT CGCTGGGTCA GCCAGAGATT TCTCTGCTAG AGAAGCTCTC CTCCCGAAGC 
TCTAGACATA TGAGGCTCAC ACCTGCTTCC CTTATGGATG AGAAGACCTC TTTGTCTCTG 
GTCAATATCA ACCCATTCAC TCCGGAGACC TACAGAAAAT TACTCCTTCA ATCAAAGGGC 
AAGAGGAAGA CCAGAGACTA TCTTGAGGAA ACTGGAGAAG AGGAAAGCAA ATCAGTACAG 
TGGCTGCCTG CTAAGAGAAG TATTCTACAA GAAACTAATA TGGCTTCCCG CTATGAAAAA 
GAATTCTTCG AGATAGAAAA AATCGGGGTT GGAGAATTTG GGACAGTCTA CAAGTGCATT 
AAGAGGCTGG ATGGATGCAT TTATGCAATA AAGCGCTCTG CAAAGTCCTT TTCAGGATTA 
TCAAATGAAT TGGATTTGCA TGAAGTTTAT GCTCATGCAG TGCTTGGCCA TCATCCCCAT 
GTGGTACGCT ACTACTCATC ATGGATAGAA GATGATCATG TGGTCATTCA AAATGAATAC 
TGTAATGGTG GGAGCCTGCA AGCTGCTATA TCTGAAAACA CTGCGTCCAA TAATCACTTC 
CAAGAGCCCA AACTCAAAGA CATCCTTCTG CAGATTTCCT TGGGACTTAA GTACATCCAC 
AATTCTGGCA TGGTACACCT GGACATCAAA CCCAGTAACA TCTTCATTTG TCATAAGATG 
CAGTGTGACT CTCCTGTAGG CCCAGAAGAG GCTGAAAGTG AAGCTGACTG GTTTCTCAAT 
GCCAGTGTGA TGTATAAAAT TGGTGACCTG GGCCACGCGA CATCAATCAG CAAACCTAAA 
GTGGAAGAAG GAGATACTCG TTTCCTGGCT AATGAGATCT TGCAAGAGAA TTACCAACAC 
CTCCCCAAAG CAGACATATT TGCCTTAGGA TTAACAATCG CAGTGGCTGC AGGAGCAGAG 
TCTTTACCCA TCAATGGTGA CATGTGGCAT CATATCCGCA AAGGGAACTT TCCAGAGATT 
TCCCAAGAGC TCTCGGATGA CTTTTATGGT CTACTCAAGA ACATGATCCA CCCAGCTCCA 
AAGGAGAGAC CATCTGCAGC AGCTCTGGCC AGAAGTCGAA TTCTCTGGCC TTTCTTGGAG 
AAGACTGATG AACTCCAGAA ACAACTGAAT TTGGAGAAAT CCAAGACAGC CACACTGAAA 
AGGGAACTGA AAAAAGCTCG GCATATCCAG ACCCCTCAGA GAGAAGTCCA CCATTGTTAT 
TATCAGATCT GCAAAGGCTC AAGACACCTC CTGGTGGGAG GAAGGAGGAA GGCGCCCTCA 
AGCTTCACCC GTGGGACATC CTCCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wee2 ( XM_006506326.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506389.1 Mus musculus WEE1 homolog 2 (S. pombe) (Wee2), transcript variant X1, mRNA.

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CloneID	OMu18090
Clone ID Related Accession (Same CDS sequence)	XM_006506326.4 , XM_006506326.3 , NM_201370.2
Accession Version	XM_006506326.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	wee1-like protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506326.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGACA CAGAGACTGA CCAGGGACTG AACAAGAAAT TAAGCTTCTC CTTTTGTGAA 
GAAGACACTG AGAGTGAAGG GCAGATGACT GCACAAGACA TCGGAGGGGC CCAGAGCCAA 
AAGCCTGGGA GGGAAGAAAG TGAGGAGTTG GAGGGTCCCG TGCCTCCTAC CAGAGATGAA 
CTTCATACTT CTCTGAGCAG AGACAAGGAA AGTCCAGGTC CTGATCTTTG GAAATCAGTT 
TCAGGCCCTC CCAAATGCCC AGAGACACCA GTATTACACA GCAAGAGCAA GCTGCCAGTC 
CCTACCAACT TCTCCACTCC CAAAAATTCG CTGGGTCAGC CAGAGATTTC TCTGCTAGAG 
AAGCTCTCCT CCCGAAGCTC TAGACATATG AGGCTCACAC CTGCTTCCCT TATGGATGAG 
AAGACCTCTT TGTCTCTGGT CAATATCAAC CCATTCACTC CGGAGACCTA CAGAAAATTA 
CTCCTTCAAT CAAAGGGCAA GAGGAAGACC AGAGACTATC TTGAGGAAAC TGGAGAAGAG 
GAAAGCAAAT CAGTACAGTG GCTGCCTGCT AAGAGAAGTA TTCTACAAGA AACTAATATG 
GCTTCCCGCT ATGAAAAAGA ATTCTTCGAG ATAGAAAAAA TCGGGGTTGG AGAATTTGGG 
ACAGTCTACA AGTGCATTAA GAGGCTGGAT GGATGCATTT ATGCAATAAA GCGCTCTGCA 
AAGTCCTTTT CAGGATTATC AAATGAATTG GATTTGCATG AAGTTTATGC TCATGCAGTG 
CTTGGCCATC ATCCCCATGT GGTACGCTAC TACTCATCAT GGATAGAAGA TGATCATGTG 
GTCATTCAAA ATGAATACTG TAATGGTGGG AGCCTGCAAG CTGCTATATC TGAAAACACT 
GCGTCCAATA ATCACTTCCA AGAGCCCAAA CTCAAAGACA TCCTTCTGCA GATTTCCTTG 
GGACTTAAGT ACATCCACAA TTCTGGCATG GTACACCTGG ACATCAAACC CAGTAACATC 
TTCATTTGTC ATAAGATGCA GTGTGACTCT CCTGTAGGCC CAGAAGAGGC TGAAAGTGAA 
GCTGACTGGT TTCTCAATGC CAGTGTGATG TATAAAATTG GTGACCTGGG CCACGCGACA 
TCAATCAGCA AACCTAAAGT GGAAGAAGGA GATACTCGTT TCCTGGCTAA TGAGATCTTG 
CAAGAGAATT ACCAACACCT CCCCAAAGCA GACATATTTG CCTTAGGATT AACAATCGCA 
GTGGCTGCAG GAGCAGAGTC TTTACCCATC AATGGTGACA TGTGGCATCA TATCCGCAAA 
GGGAACTTTC CAGAGATTTC CCAAGAGCTC TCGGATGACT TTTATGGTCT ACTCAAGAAC 
ATGATCCACC CAGCTCCAAA GGAGAGACCA TCTGCAGCAG CTCTGGCCAG AAGTCGAATT 
CTCTGGCCTT TCTTGGAGAA GACTGATGAA CTCCAGAAAC AACTGAATTT GGAGAAATCC 
AAGACAGCCA CACTGAAAAG GGAACTGAAA AAAGCTCGGC ATATCCAGAC CCCTCAGAGA 
GAAGTCCACC ATTGTTATTA TCAGATCTGC AAAGGCTCAA GACACCTCCT GGTGGGAGGA 
AGGAGGAAGG CGCCCTCAAG CTTCACCCGT GGGACATCCT CCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506389.1
CDS	544..2211
Translation	MADTETDQGLNKKLSFSFCEEDTESEGQMTAQDIGGAQSQKPGREESEELEGPVPPTRDELHTSLSRDKESPGPDLWKSVSGPPKCPETPVLHSKSKLPVPTNFSTPKNSLGQPEISLLEKLSSRSSRHMRLTPASLMDEKTSLSLVNINPFTPETYRKLLLQSKGKRKTRDYLEETGEEESKSVQWLPAKRSILQETNMASRYEKEFFEIEKIGVGEFGTVYKCIKRLDGCIYAIKRSAKSFSGLSNELDLHEVYAHAVLGHHPHVVRYYSSWIEDDHVVIQNEYCNGGSLQAAISENTASNNHFQEPKLKDILLQISLGLKYIHNSGMVHLDIKPSNIFICHKMQCDSPVGPEEAESEADWFLNASVMYKIGDLGHATSISKPKVEEGDTRFLANEILQENYQHLPKADIFALGLTIAVAAGAESLPINGDMWHHIRKGNFPEISQELSDDFYGLLKNMIHPAPKERPSAAALARSRILWPFLEKTDELQKQLNLEKSKTATLKRELKKARHIQTPQREVHHCYYQICKGSRHLLVGGRRKAPSSFTRGTSSV

Target ORF information:

RefSeq Version	XM_006506326.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus WEE1 homolog 2 (S. pombe) (Wee2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506326.4

ORF Insert Sequence:

ATGGCCGACA CAGAGACTGA CCAGGGACTG AACAAGAAAT TAAGCTTCTC CTTTTGTGAA 
GAAGACACTG AGAGTGAAGG GCAGATGACT GCACAAGACA TCGGAGGGGC CCAGAGCCAA 
AAGCCTGGGA GGGAAGAAAG TGAGGAGTTG GAGGGTCCCG TGCCTCCTAC CAGAGATGAA 
CTTCATACTT CTCTGAGCAG AGACAAGGAA AGTCCAGGTC CTGATCTTTG GAAATCAGTT 
TCAGGCCCTC CCAAATGCCC AGAGACACCA GTATTACACA GCAAGAGCAA GCTGCCAGTC 
CCTACCAACT TCTCCACTCC CAAAAATTCG CTGGGTCAGC CAGAGATTTC TCTGCTAGAG 
AAGCTCTCCT CCCGAAGCTC TAGACATATG AGGCTCACAC CTGCTTCCCT TATGGATGAG 
AAGACCTCTT TGTCTCTGGT CAATATCAAC CCATTCACTC CGGAGACCTA CAGAAAATTA 
CTCCTTCAAT CAAAGGGCAA GAGGAAGACC AGAGACTATC TTGAGGAAAC TGGAGAAGAG 
GAAAGCAAAT CAGTACAGTG GCTGCCTGCT AAGAGAAGTA TTCTACAAGA AACTAATATG 
GCTTCCCGCT ATGAAAAAGA ATTCTTCGAG ATAGAAAAAA TCGGGGTTGG AGAATTTGGG 
ACAGTCTACA AGTGCATTAA GAGGCTGGAT GGATGCATTT ATGCAATAAA GCGCTCTGCA 
AAGTCCTTTT CAGGATTATC AAATGAATTG GATTTGCATG AAGTTTATGC TCATGCAGTG 
CTTGGCCATC ATCCCCATGT GGTACGCTAC TACTCATCAT GGATAGAAGA TGATCATGTG 
GTCATTCAAA ATGAATACTG TAATGGTGGG AGCCTGCAAG CTGCTATATC TGAAAACACT 
GCGTCCAATA ATCACTTCCA AGAGCCCAAA CTCAAAGACA TCCTTCTGCA GATTTCCTTG 
GGACTTAAGT ACATCCACAA TTCTGGCATG GTACACCTGG ACATCAAACC CAGTAACATC 
TTCATTTGTC ATAAGATGCA GTGTGACTCT CCTGTAGGCC CAGAAGAGGC TGAAAGTGAA 
GCTGACTGGT TTCTCAATGC CAGTGTGATG TATAAAATTG GTGACCTGGG CCACGCGACA 
TCAATCAGCA AACCTAAAGT GGAAGAAGGA GATACTCGTT TCCTGGCTAA TGAGATCTTG 
CAAGAGAATT ACCAACACCT CCCCAAAGCA GACATATTTG CCTTAGGATT AACAATCGCA 
GTGGCTGCAG GAGCAGAGTC TTTACCCATC AATGGTGACA TGTGGCATCA TATCCGCAAA 
GGGAACTTTC CAGAGATTTC CCAAGAGCTC TCGGATGACT TTTATGGTCT ACTCAAGAAC 
ATGATCCACC CAGCTCCAAA GGAGAGACCA TCTGCAGCAG CTCTGGCCAG AAGTCGAATT 
CTCTGGCCTT TCTTGGAGAA GACTGATGAA CTCCAGAAAC AACTGAATTT GGAGAAATCC 
AAGACAGCCA CACTGAAAAG GGAACTGAAA AAAGCTCGGC ATATCCAGAC CCCTCAGAGA 
GAAGTCCACC ATTGTTATTA TCAGATCTGC AAAGGCTCAA GACACCTCCT GGTGGGAGGA 
AGGAGGAAGG CGCCCTCAAG CTTCACCCGT GGGACATCCT CCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins.
Science (New York, N.Y.)342(6165)1518-21(2013 Dec)
Yu C,Zhang YL,Pan WW,Li XM,Wang ZW,Ge ZJ,Zhou JJ,Cang Y,Tong C,Sun QY,Fan HY

Ser 15 of WEE1B is a potential PKA phosphorylation target in G2/M transition in one-cell stage mouse embryos.
Molecular medicine reports7(6)1929-37(2013 Jun)
Liu C,Liu Y,Liu Y,Wu D,Luan Z,Wang E,Yu B

MARF1 regulates essential oogenic processes in mice.
Science (New York, N.Y.)335(6075)1496-9(2012 Mar)
Su YQ,Sugiura K,Sun F,Pendola JK,Cox GA,Handel MA,Schimenti JC,Eppig JJ

Protein tyrosine kinase Wee1B is essential for metaphase II exit in mouse oocytes.
Science (New York, N.Y.)332(6028)462-5(2011 Apr)
Oh JS,Susor A,Conti M

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Wee2 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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