Cartpt cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cartpt
Entrez Gene ID	27220
Full Name	CART prepropeptide
Synonyms	Cart
General protein information	Preferred Names CART prepropeptide Names cocaine- and amphetamine-regulated transcript protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 D1
Summary	This gene encodes preproprotein isoforms that are processed into multiple biologically active peptides. Expression of this gene is regulated by cocaine and other drugs, and is associated with feeding/appetite and stress response. Mice lacking the encoded protein are predisposed to obesity. Deficiency of the encoded protein in mice results in pancreatic islet dysfunction, impaired insulin secretion and glucose intolerance. Alternative splicing results in multiple transcript variants encoding different isoforms, which are subsequently processed into mature peptides. [provided by RefSeq, Jul 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_030247291.1	XP_030103151.1	cocaine- and amphetamine-regulated transcript protein isoform X1
NM_013732.7	NP_038760.3	cocaine- and amphetamine-regulated transcript protein isoform 1 preproprotein
NM_001081493.2	NP_001074962.1	cocaine- and amphetamine-regulated transcript protein isoform 2 preproprotein

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cartpt	NP_058806.1
Xenopus tropicalis (tropical clawed frog)	cartpt	XP_002934864.1
Bos taurus (cattle)	CARTPT	NP_001007821.1
Mus musculus (house mouse)	Cartpt	NP_038760.3
	CARTPT	XP_001096906.1
Canis lupus familiaris (dog)	CARTPT	XP_852772.1
Pan troglodytes (chimpanzee)	CARTPT	XP_001155069.1
Homo sapiens (human)	CARTPT	NP_004282.1

Gene Ontology
Bibliography

Related articles in PubMed

Ancestral Circuits for the Coordinated Modulation of Brain State.
Lovett-Barron M, Andalman AS, Allen WE, Vesuna S, Kauvar I, Burns VM, Deisseroth K
Cell171(6)1411-1423.e17(2017 Nov)

Processing of cocaine- and amphetamine-regulated transcript (CART) precursor proteins by prohormone convertases (PCs) and its implications.
Stein J, Steiner DF, Dey A
Peptides27(8)1919-25(2006 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Taken together, these results demonstrate that CART is a critical regulator of energy expenditure, energy partitioning and utilization dependent on the thermal environment.
Title: Temperature dependence of the control of energy homeostasis requires CART signaling.

Study conclude that CART is a regulator of glucose homeostasis and could play an important role in the pathophysiology of type 2 diabetes. Based on the ability of CART to increase insulin secretion and reduce glucagon secretion, CART-based agents could be a therapeutic modality in type 2 diabetes.
Title: CART is overexpressed in human type 2 diabetic islets and inhibits glucagon secretion and increases insulin secretion.

Study showed that the perikarya and central terminals of vagal afferents are invariably enriched in CART and devoid of melanin-concentrating hormone and that neuropeptidergic profile of the CART-positive vagal afferents is remarkably stable across the energy balance spectrum.
Title: Levels of Cocaine- and Amphetamine-Regulated Transcript in Vagal Afferents in the Mouse Are Unaltered in Response to Metabolic Challenges.

Collectively, our main findings indicate that high-fat diet induced-hypertension and autonomic imbalance are associated to an upregulation of CART levels in the dorsomedial hypothalamus of mice.
Title: High-fat diet-induced hypertension and autonomic imbalance are associated with an upregulation of CART in the dorsomedial hypothalamus of mice.

Leptin induced CART negatively affects intracellular cAMP levels, MAPK signaling, and aromatase mRNA expression, which leads to lower estradiol synthesis in granulosa cells and altered ovarian folliculogenesis.
Title: Leptin-Induced CART (Cocaine- and Amphetamine-Regulated Transcript) Is a Novel Intraovarian Mediator of Obesity-Related Infertility in Females.

The following Cartpt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cartpt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu23329	NM_001081493.2 Latest version!	Mus musculus CART prepropeptide (Cartpt), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu17505	NM_013732.7 Latest version!	Mus musculus CART prepropeptide (Cartpt), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu23329	XM_030247291.1 Latest version!	Mus musculus CART prepropeptide (Cartpt), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cartpt ( NM_001081493.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074962.1 Mus musculus CART prepropeptide (Cartpt), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu23329
Clone ID Related Accession (Same CDS sequence)	XM_030247291.1 , NM_001081493.2
Accession Version	NM_001081493.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	cocaine- and amphetamine-regulated transcript protein isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CO427341.1, AK134656.1 and AV341177.1. On Oct 4, 2012 this sequence version replaced NM_001081493.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK134656.1, CJ049927.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849381, SAMN01164131 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAGCT CCCGCCTGCG GCTGCTACCC CTCCTGGGCG CCGCCCTGCT GCTACTGCTA 
CCTTTGCTGG GTGCCCGTGC CCAGGAGGAC GCCGAGCTGC AGCCCCGAGC CCTGGACATC 
TACTCTGCCG TGGATGATGC GTCCCACGAG AAGGAGCTGA TCGAAGCGTT GCAAGAAGTC 
CTGAAGAAGC TCAAGAGTAA ACGCATTCCG ATCTACGAGA AGAAGTACGG CCAAGTCCCC 
ATGTGTGACG CTGGAGAGCA GTGCGCAGTG AGGAAAGGGG CCAGGATCGG GAAGCTGTGT 
GACTGTCCCC GAGGAACTTC CTGCAATTCT TTCCTCTTGA AGTGCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074962.1
CDS	50..400
Translation	MESSRLRLLPLLGAALLLLLPLLGARAQEDAELQPRALDIYSAVDDASHEKELIEALQEVLKKLKSKRIPIYEKKYGQVPMCDAGEQCAVRKGARIGKLCDCPRGTSCNSFLLKCL

Target ORF information:

RefSeq Version	NM_001081493.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus CART prepropeptide (Cartpt), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081493.2

ORF Insert Sequence:

ATGGAGAGCT CCCGCCTGCG GCTGCTACCC CTCCTGGGCG CCGCCCTGCT GCTACTGCTA 
CCTTTGCTGG GTGCCCGTGC CCAGGAGGAC GCCGAGCTGC AGCCCCGAGC CCTGGACATC 
TACTCTGCCG TGGATGATGC GTCCCACGAG AAGGAGCTGA TCGAAGCGTT GCAAGAAGTC 
CTGAAGAAGC TCAAGAGTAA ACGCATTCCG ATCTACGAGA AGAAGTACGG CCAAGTCCCC 
ATGTGTGACG CTGGAGAGCA GTGCGCAGTG AGGAAAGGGG CCAGGATCGG GAAGCTGTGT 
GACTGTCCCC GAGGAACTTC CTGCAATTCT TTCCTCTTGA AGTGCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cartpt ( NM_013732.7 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038760.3 Mus musculus CART prepropeptide (Cartpt), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu17505
Clone ID Related Accession (Same CDS sequence)	NM_013732.7
Accession Version	NM_013732.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	cocaine- and amphetamine-regulated transcript protein isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CO427341.1, BC056431.1, AK134656.1 and AV341177.1. On Oct 4, 2012 this sequence version replaced NM_013732.6. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AU079813.1, CK627725.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAGCT CCCGCCTGCG GCTGCTACCC CTCCTGGGCG CCGCCCTGCT GCTACTGCTA 
CCTTTGCTGG GTGCCCGTGC CCAGGAGGAC GCCGAGCTGC AGCCCCGAGC CCTGGACATC 
TACTCTGCCG TGGATGATGC GTCCCACGAG AAGGAGCTGC CAAGGCGGCA ACTTCGGGCT 
CCCGGCGCTA TGTTGCAGAT CGAAGCGTTG CAAGAAGTCC TGAAGAAGCT CAAGAGTAAA 
CGCATTCCGA TCTACGAGAA GAAGTACGGC CAAGTCCCCA TGTGTGACGC TGGAGAGCAG 
TGCGCAGTGA GGAAAGGGGC CAGGATCGGG AAGCTGTGTG ACTGTCCCCG AGGAACTTCC 
TGCAATTCTT TCCTCTTGAA GTGCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038760.3
CDS	50..439
Translation	MESSRLRLLPLLGAALLLLLPLLGARAQEDAELQPRALDIYSAVDDASHEKELPRRQLRAPGAMLQIEALQEVLKKLKSKRIPIYEKKYGQVPMCDAGEQCAVRKGARIGKLCDCPRGTSCNSFLLKCL

Target ORF information:

RefSeq Version	NM_013732.7
Organism	Mus musculus(house mouse)
Definition	Mus musculus CART prepropeptide (Cartpt), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013732.7

ORF Insert Sequence:

ATGGAGAGCT CCCGCCTGCG GCTGCTACCC CTCCTGGGCG CCGCCCTGCT GCTACTGCTA 
CCTTTGCTGG GTGCCCGTGC CCAGGAGGAC GCCGAGCTGC AGCCCCGAGC CCTGGACATC 
TACTCTGCCG TGGATGATGC GTCCCACGAG AAGGAGCTGC CAAGGCGGCA ACTTCGGGCT 
CCCGGCGCTA TGTTGCAGAT CGAAGCGTTG CAAGAAGTCC TGAAGAAGCT CAAGAGTAAA 
CGCATTCCGA TCTACGAGAA GAAGTACGGC CAAGTCCCCA TGTGTGACGC TGGAGAGCAG 
TGCGCAGTGA GGAAAGGGGC CAGGATCGGG AAGCTGTGTG ACTGTCCCCG AGGAACTTCC 
TGCAATTCTT TCCTCTTGAA GTGCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cartpt ( XM_030247291.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103151.1 Mus musculus CART prepropeptide (Cartpt), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu23329
Clone ID Related Accession (Same CDS sequence)	XM_030247291.1 , NM_001081493.2
Accession Version	XM_030247291.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cocaine- and amphetamine-regulated transcript protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGCT CCCGCCTGCG GCTGCTACCC CTCCTGGGCG CCGCCCTGCT GCTACTGCTA 
CCTTTGCTGG GTGCCCGTGC CCAGGAGGAC GCCGAGCTGC AGCCCCGAGC CCTGGACATC 
TACTCTGCCG TGGATGATGC GTCCCACGAG AAGGAGCTGA TCGAAGCGTT GCAAGAAGTC 
CTGAAGAAGC TCAAGAGTAA ACGCATTCCG ATCTACGAGA AGAAGTACGG CCAAGTCCCC 
ATGTGTGACG CTGGAGAGCA GTGCGCAGTG AGGAAAGGGG CCAGGATCGG GAAGCTGTGT 
GACTGTCCCC GAGGAACTTC CTGCAATTCT TTCCTCTTGA AGTGCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103151.1
CDS	167..517
Translation	MESSRLRLLPLLGAALLLLLPLLGARAQEDAELQPRALDIYSAVDDASHEKELIEALQEVLKKLKSKRIPIYEKKYGQVPMCDAGEQCAVRKGARIGKLCDCPRGTSCNSFLLKCL

Target ORF information:

RefSeq Version	XM_030247291.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CART prepropeptide (Cartpt), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247291.1

ORF Insert Sequence:

ATGGAGAGCT CCCGCCTGCG GCTGCTACCC CTCCTGGGCG CCGCCCTGCT GCTACTGCTA 
CCTTTGCTGG GTGCCCGTGC CCAGGAGGAC GCCGAGCTGC AGCCCCGAGC CCTGGACATC 
TACTCTGCCG TGGATGATGC GTCCCACGAG AAGGAGCTGA TCGAAGCGTT GCAAGAAGTC 
CTGAAGAAGC TCAAGAGTAA ACGCATTCCG ATCTACGAGA AGAAGTACGG CCAAGTCCCC 
ATGTGTGACG CTGGAGAGCA GTGCGCAGTG AGGAAAGGGG CCAGGATCGG GAAGCTGTGT 
GACTGTCCCC GAGGAACTTC CTGCAATTCT TTCCTCTTGA AGTGCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ancestral Circuits for the Coordinated Modulation of Brain State.
Cell171(6)1411-1423.e17(2017 Nov)
Lovett-Barron M,Andalman AS,Allen WE,Vesuna S,Kauvar I,Burns VM,Deisseroth K

Processing of cocaine- and amphetamine-regulated transcript (CART) precursor proteins by prohormone convertases (PCs) and its implications.
Peptides27(8)1919-25(2006 Aug)
Stein J,Steiner DF,Dey A

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Cartpt cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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