Ncdn cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ncdn
Entrez Gene ID	26562
Full Name	neurochondrin
Synonyms	AU042419,MMS10-AE,Ms10ae,mKIAA0607,norbin
General protein information	Preferred Names neurochondrin Names neurochondrin M-Sema F-associating protein of 75 kDa minisatellite 10ae detected by probe MMS10 neurochondrin-1 neurochondrin-2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D2.2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006503119.3	XP_006503182.1	neurochondrin isoform X1
XM_006503118.3	XP_006503181.1	neurochondrin isoform X1
NM_001355413.1	NP_001342342.1	neurochondrin isoform 2
XM_030253580.1	XP_030109440.1	neurochondrin isoform X1
NM_011986.4	NP_036116.3	neurochondrin isoform 1
NM_001355412.1	NP_001342341.1	neurochondrin isoform 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NCDN	XP_532565.2
Pan troglodytes (chimpanzee)	NCDN	XP_003308021.1
	NCDN	XP_002802364.1
Xenopus tropicalis (tropical clawed frog)	ncdn	XP_002938879.1
Homo sapiens (human)	NCDN	NP_001014839.1
Gallus gallus (chicken)	NCDN	NP_001026072.1
Danio rerio (zebrafish)	ncdn	XP_002667129.1
Drosophila melanogaster (fruit fly)	Neurochondrin	NP_649658.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP013338	XP_003436800.1
Rattus norvegicus (Norway rat)	Ncdn	NP_445995.2
Bos taurus (cattle)	NCDN	NP_001039396.1
Mus musculus (house mouse)	Ncdn	NP_036116.3

Gene Ontology
Bibliography

Related articles in PubMed

Norbin Stimulates the Catalytic Activity and Plasma Membrane Localization of the Guanine-Nucleotide Exchange Factor P-Rex1.
Pan D, Barber MA, Hornigold K, Baker MJ, Toth JM, Oxley D, Welch HC
The Journal of biological chemistry291(12)6359-75(2016 Mar)

Norbin ablation results in defective adult hippocampal neurogenesis and depressive-like behavior in mice.
Wang H, Warner-Schmidt J, Varela S, Enikolopov G, Greengard P, Flajolet M
Proceedings of the National Academy of Sciences of the United States of America112(31)9745-50(2015 Aug)

In silico screening for palmitoyl substrates reveals a role for DHHC1/3/10 (zDHHC1/3/11)-mediated neurochondrin palmitoylation in its targeting to Rab5-positive endosomes.
Oku S, Takahashi N, Fukata Y, Fukata M
The Journal of biological chemistry288(27)19816-29(2013 Jul)

Norbin is an endogenous regulator of metabotropic glutamate receptor 5 signaling.
Wang H, Westin L, Nong Y, Birnbaum S, Bendor J, Brismar H, Nestler E, Aperia A, Flajolet M, Greengard P
Science (New York, N.Y.)326(5959)1554-7(2009 Dec)

The level of the transcription factor Pax6 is essential for controlling the balance between neural stem cell self-renewal and neurogenesis.
Sansom SN, Griffiths DS, Faedo A, Kleinjan DJ, Ruan Y, Smith J, van Heyningen V, Rubenstein JL, Livesey FJ
PLoS genetics5(6)e1000511(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A novel mechanism of P-Rex1 regulation through the GPCR-adaptor protein Norbin.
Title: Norbin Stimulates the Catalytic Activity and Plasma Membrane Localization of the Guanine-Nucleotide Exchange Factor P-Rex1.

Norbin is a regulator of adult hippocampal neurogenesis and that its deletion causes depressive-like behaviors.
Title: Norbin ablation results in defective adult hippocampal neurogenesis and depressive-like behavior in mice.

In silico screening for palmitoyl substrates reveals a role for DHHC1/3/10 (zDHHC1/3/11)-mediated neurochondrin palmitoylation in its targeting to Rab5-positive endosomes.
Title: In silico screening for palmitoyl substrates reveals a role for DHHC1/3/10 (zDHHC1/3/11)-mediated neurochondrin palmitoylation in its targeting to Rab5-positive endosomes.

study reports that Norbin, a neuron-specific protein, physically interacts with mGluR5 in vivo, increases the cell surface localization of the receptor, and positively regulates mGluR5 signaling
Title: Norbin is an endogenous regulator of metabotropic glutamate receptor 5 signaling.

results showed that neurochondrin is a negative regulator of Ca(2+)/calmodulin-dependent protein kinase II phosphorylation and specific homozygous gene disruption resulted in epileptic seizure
Title: Neurochondrin negatively regulates CaMKII phosphorylation, and nervous system-specific gene disruption results in epileptic seizure.

The following Ncdn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ncdn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09276	NM_011986.4 Latest version!	Mus musculus neurochondrin (Ncdn), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu33605	NM_001355413.1 Latest version!	Mus musculus neurochondrin (Ncdn), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu33605	NM_001355412.1 Latest version!	Mus musculus neurochondrin (Ncdn), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu33605	XM_006503118.3 Latest version!	Mus musculus neurochondrin (Ncdn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu33605	XM_006503119.3 Latest version!	Mus musculus neurochondrin (Ncdn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu88544	XM_030253580.1 Latest version!	Mus musculus neurochondrin (Ncdn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ncdn ( NM_011986.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036116.3 Mus musculus neurochondrin (Ncdn), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu09276
Clone ID Related Accession (Same CDS sequence)	NM_011986.4
Accession Version	NM_011986.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	neurochondrin isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB855959.1, CD548169.1, BQ745150.1, CX568560.1, AK002938.1 and AL606908.10. On Jul 10, 2009 this sequence version replaced NM_011986.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK154889.1, SRR1660817.165687.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGTGTT GTGACCTGGC TGCGGCGGGA CAGTTGGGCA AGGCGGGCAT CATGGCCTCG 
GATTGTGAGC CAGCTCTGAA CCAGGCAGAG AGCCGAAACC CCACCCTGGA GCGCTACCTG 
GGAGCCCTCC GTGAGGCCAA GAATGACAGC GAGCAGTTTG CAGCCCTGCT GCTAGTAACG 
AAGGCAGTCA AAGCAGGTGA CATTGATGCC AAAACCCGAC GTAGGATCTT TGATGCCGTT 
GGGTTCACCT TTCCCAACCG ACTCCTGACC ACTAAGGAGG CACCTGATGG CTGCCCTGAC 
CACGTTCTCC GGGCCCTGGG CGTGGCCCTG CTGGCCTGTT TCTGCAGCGA CCCTGAACTA 
GCCAGCCATC CCCAGGTCCT GAACAAGATC CCCATCCTTA GCACATTCCT TACAGCCCGG 
GGGGACCCTG ATGATGCTGC CCGCCGCTCC ATGATCGATG ACACCTACCA GTGCCTGACA 
GCTGTTGCGG GCACACCCCG AGGGCCCCGG CACCTCATTG CTGGTGGCAC CGTGTCTGCC 
CTGTGCCAGG CGTACCTGGG GCATGGCTAC GGCTTTGACC AGGCTCTGGC ACTCTTGGTG 
GGGCTGCTGG CTGCTGCAGA GACACAGTGC TGGAAGGAGG CGGAGCCCGA CCTGCTGGCT 
GTGTTGCGAG GCCTCAGTGA GGATTTCCAA AGAGCCGAAG ATGCCAGCAA GTTTGAGCTC 
TGCCAGCTGC TGCCCCTTTT TCTGCCCCCA ACAACTGTGC CCCCTGAATG CCACCGAGAT 
CTGCAGGCTG GGCTGGCACG AATCCTAGGA AGCAAGTTGA GCTCCTGGCA GCGCAACCCT 
GCACTGAAGC TGGCAGCCCG CCTGGCTCAT GCCTGCGGCT CCGACTGGAT CCCAGTGGGC 
AGCTCTGGGA GCAAGTTTCT GGCCCTGCTC GTGAATCTAG CGTGCGTGGA GGTACGGCTG 
GCTCTCGAGG AGACAGGCAC AGAGGTGAAA GAAGACGTGG TAACAGCCTG CTATGCCCTT 
ATGGAATTGG GGATCCAGGA GTGCACCCGC TGTGAGCAGT CCCTGCTTAA GGAGCCACAG 
AAGGTTCAGC TCGTGAGCAT TATGAAAGAG GCCATTGGCG CTGTCATCCA CTACCTGCTG 
CAGGTGGGGC CAGAGAAGCA GAAAGAGCCC TTTGTGTTTG CCTCGGTGCG GATCCTGGGT 
GCCTGGCTGG CGGAGGAGAC CTCATCCCTG CGTAAGGAGG TGTGCCAACT GCTGCCCTTC 
CTTGTCCGAT ATGCCAAGAC ACTCTATGAG GAGGCTGAGG AGGCCAGTGA CATTTCGCAG 
CAGGTGGCCA ACTTGGCCAT CTCTCCTACT ACACCAGGGC CTTCATGGCC AGGGGATGCT 
CTCCGGCTCC TCCTTCCCGG CTGGTGTCAC CTGACTGTTG AAGATGGTCC CCGGGAGATT 
CTGATCAAGG AAGGAGCCCC CTCACTTCTG TGCAAGTACT TCCTGCAGCA GTGGGAACTC 
ACATCCCCGG GCCATGATAC CTCGGTGCTG CCAGACAGCG TGGAGATCGG CCTTCAGACC 
TGTTGCCACA TCTTCCTCAA CCTGGTGGTC ACCGCTCCAG GGCTGATCAA ACGCGATGCC 
TGCTTCACAT CTCTTATGAA CACCCTGATG ACGTCACTGC CCTCACTAGT GCAGCAACAA 
GGGAGACTGC TTCTAGCTGC CAACGTGGCC ACTTTGGGGC TCCTAATGGC CCGGCTCCTT 
AGCACCTCTC CAGCTCTCCA AGGAACCCCA GCCTCCCGAG GTTTCTTCGC AGCTGCCATC 
CTCTTTCTGT CACAGTCCCA TGTGGCACGA GCCACCCCTG GCTCTGACCA GGCAGTGTTG 
GCCCTGTCCC CTGACTATGA AGGCATCTGG GCTGACTTGC AAGAGCTCTG GTTCCTGGGC 
ATGCAGGCCT TCACGGGTTG TGTGCCGCTG CTGCCCTGGC TGGCCCCTGC CGCCCTGCGC 
TCCCGCTGGC CACAGGAGCT GCTACAACTG CTAGGTAGTG TAAGCCCCAA CTCCGTCAAG 
CCTGAGATGG TGGCTGCCTA CCAGGGCGTG CTGGTGGAAT TGGCACGGGC TAACCGGCTA 
TGCCGGGAGG CCATGAGGCT GCAGGCGGGT GAAGAAACGG CCAGCCATTA CCGAATGGCT 
GCTTTGGAGC AGTGCCTGTC AGAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036116.3
CDS	427..2616
Translation	MSCCDLAAAGQLGKAGIMASDCEPALNQAESRNPTLERYLGALREAKNDSEQFAALLLVTKAVKAGDIDAKTRRRIFDAVGFTFPNRLLTTKEAPDGCPDHVLRALGVALLACFCSDPELASHPQVLNKIPILSTFLTARGDPDDAARRSMIDDTYQCLTAVAGTPRGPRHLIAGGTVSALCQAYLGHGYGFDQALALLVGLLAAAETQCWKEAEPDLLAVLRGLSEDFQRAEDASKFELCQLLPLFLPPTTVPPECHRDLQAGLARILGSKLSSWQRNPALKLAARLAHACGSDWIPVGSSGSKFLALLVNLACVEVRLALEETGTEVKEDVVTACYALMELGIQECTRCEQSLLKEPQKVQLVSIMKEAIGAVIHYLLQVGPEKQKEPFVFASVRILGAWLAEETSSLRKEVCQLLPFLVRYAKTLYEEAEEASDISQQVANLAISPTTPGPSWPGDALRLLLPGWCHLTVEDGPREILIKEGAPSLLCKYFLQQWELTSPGHDTSVLPDSVEIGLQTCCHIFLNLVVTAPGLIKRDACFTSLMNTLMTSLPSLVQQQGRLLLAANVATLGLLMARLLSTSPALQGTPASRGFFAAAILFLSQSHVARATPGSDQAVLALSPDYEGIWADLQELWFLGMQAFTGCVPLLPWLAPAALRSRWPQELLQLLGSVSPNSVKPEMVAAYQGVLVELARANRLCREAMRLQAGEETASHYRMAALEQCLSEP

Target ORF information:

RefSeq Version	NM_011986.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurochondrin (Ncdn), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011986.4

ORF Insert Sequence:

ATGTCGTGTT GTGACCTGGC TGCGGCGGGA CAGTTGGGCA AGGCGGGCAT CATGGCCTCG 
GATTGTGAGC CAGCTCTGAA CCAGGCAGAG AGCCGAAACC CCACCCTGGA GCGCTACCTG 
GGAGCCCTCC GTGAGGCCAA GAATGACAGC GAGCAGTTTG CAGCCCTGCT GCTAGTAACG 
AAGGCAGTCA AAGCAGGTGA CATTGATGCC AAAACCCGAC GTAGGATCTT TGATGCCGTT 
GGGTTCACCT TTCCCAACCG ACTCCTGACC ACTAAGGAGG CACCTGATGG CTGCCCTGAC 
CACGTTCTCC GGGCCCTGGG CGTGGCCCTG CTGGCCTGTT TCTGCAGCGA CCCTGAACTA 
GCCAGCCATC CCCAGGTCCT GAACAAGATC CCCATCCTTA GCACATTCCT TACAGCCCGG 
GGGGACCCTG ATGATGCTGC CCGCCGCTCC ATGATCGATG ACACCTACCA GTGCCTGACA 
GCTGTTGCGG GCACACCCCG AGGGCCCCGG CACCTCATTG CTGGTGGCAC CGTGTCTGCC 
CTGTGCCAGG CGTACCTGGG GCATGGCTAC GGCTTTGACC AGGCTCTGGC ACTCTTGGTG 
GGGCTGCTGG CTGCTGCAGA GACACAGTGC TGGAAGGAGG CGGAGCCCGA CCTGCTGGCT 
GTGTTGCGAG GCCTCAGTGA GGATTTCCAA AGAGCCGAAG ATGCCAGCAA GTTTGAGCTC 
TGCCAGCTGC TGCCCCTTTT TCTGCCCCCA ACAACTGTGC CCCCTGAATG CCACCGAGAT 
CTGCAGGCTG GGCTGGCACG AATCCTAGGA AGCAAGTTGA GCTCCTGGCA GCGCAACCCT 
GCACTGAAGC TGGCAGCCCG CCTGGCTCAT GCCTGCGGCT CCGACTGGAT CCCAGTGGGC 
AGCTCTGGGA GCAAGTTTCT GGCCCTGCTC GTGAATCTAG CGTGCGTGGA GGTACGGCTG 
GCTCTCGAGG AGACAGGCAC AGAGGTGAAA GAAGACGTGG TAACAGCCTG CTATGCCCTT 
ATGGAATTGG GGATCCAGGA GTGCACCCGC TGTGAGCAGT CCCTGCTTAA GGAGCCACAG 
AAGGTTCAGC TCGTGAGCAT TATGAAAGAG GCCATTGGCG CTGTCATCCA CTACCTGCTG 
CAGGTGGGGC CAGAGAAGCA GAAAGAGCCC TTTGTGTTTG CCTCGGTGCG GATCCTGGGT 
GCCTGGCTGG CGGAGGAGAC CTCATCCCTG CGTAAGGAGG TGTGCCAACT GCTGCCCTTC 
CTTGTCCGAT ATGCCAAGAC ACTCTATGAG GAGGCTGAGG AGGCCAGTGA CATTTCGCAG 
CAGGTGGCCA ACTTGGCCAT CTCTCCTACT ACACCAGGGC CTTCATGGCC AGGGGATGCT 
CTCCGGCTCC TCCTTCCCGG CTGGTGTCAC CTGACTGTTG AAGATGGTCC CCGGGAGATT 
CTGATCAAGG AAGGAGCCCC CTCACTTCTG TGCAAGTACT TCCTGCAGCA GTGGGAACTC 
ACATCCCCGG GCCATGATAC CTCGGTGCTG CCAGACAGCG TGGAGATCGG CCTTCAGACC 
TGTTGCCACA TCTTCCTCAA CCTGGTGGTC ACCGCTCCAG GGCTGATCAA ACGCGATGCC 
TGCTTCACAT CTCTTATGAA CACCCTGATG ACGTCACTGC CCTCACTAGT GCAGCAACAA 
GGGAGACTGC TTCTAGCTGC CAACGTGGCC ACTTTGGGGC TCCTAATGGC CCGGCTCCTT 
AGCACCTCTC CAGCTCTCCA AGGAACCCCA GCCTCCCGAG GTTTCTTCGC AGCTGCCATC 
CTCTTTCTGT CACAGTCCCA TGTGGCACGA GCCACCCCTG GCTCTGACCA GGCAGTGTTG 
GCCCTGTCCC CTGACTATGA AGGCATCTGG GCTGACTTGC AAGAGCTCTG GTTCCTGGGC 
ATGCAGGCCT TCACGGGTTG TGTGCCGCTG CTGCCCTGGC TGGCCCCTGC CGCCCTGCGC 
TCCCGCTGGC CACAGGAGCT GCTACAACTG CTAGGTAGTG TAAGCCCCAA CTCCGTCAAG 
CCTGAGATGG TGGCTGCCTA CCAGGGCGTG CTGGTGGAAT TGGCACGGGC TAACCGGCTA 
TGCCGGGAGG CCATGAGGCT GCAGGCGGGT GAAGAAACGG CCAGCCATTA CCGAATGGCT 
GCTTTGGAGC AGTGCCTGTC AGAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncdn ( NM_001355413.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001342342.1 Mus musculus neurochondrin (Ncdn), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu33605
Clone ID Related Accession (Same CDS sequence)	XM_006503119.3 , XM_006503118.3 , NM_001355413.1 , NM_001355412.1
Accession Version	NM_001355413.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	neurochondrin isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DN173821.1, BQ745150.1, CX568560.1, AK002938.1, AL606908.10 and BE985399.1. On Sep 15, 2017 this sequence version replaced XM_006503119.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.128208.1, SRR1660815.66995.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGG ATTGTGAGCC AGCTCTGAAC CAGGCAGAGA GCCGAAACCC CACCCTGGAG 
CGCTACCTGG GAGCCCTCCG TGAGGCCAAG AATGACAGCG AGCAGTTTGC AGCCCTGCTG 
CTAGTAACGA AGGCAGTCAA AGCAGGTGAC ATTGATGCCA AAACCCGACG TAGGATCTTT 
GATGCCGTTG GGTTCACCTT TCCCAACCGA CTCCTGACCA CTAAGGAGGC ACCTGATGGC 
TGCCCTGACC ACGTTCTCCG GGCCCTGGGC GTGGCCCTGC TGGCCTGTTT CTGCAGCGAC 
CCTGAACTAG CCAGCCATCC CCAGGTCCTG AACAAGATCC CCATCCTTAG CACATTCCTT 
ACAGCCCGGG GGGACCCTGA TGATGCTGCC CGCCGCTCCA TGATCGATGA CACCTACCAG 
TGCCTGACAG CTGTTGCGGG CACACCCCGA GGGCCCCGGC ACCTCATTGC TGGTGGCACC 
GTGTCTGCCC TGTGCCAGGC GTACCTGGGG CATGGCTACG GCTTTGACCA GGCTCTGGCA 
CTCTTGGTGG GGCTGCTGGC TGCTGCAGAG ACACAGTGCT GGAAGGAGGC GGAGCCCGAC 
CTGCTGGCTG TGTTGCGAGG CCTCAGTGAG GATTTCCAAA GAGCCGAAGA TGCCAGCAAG 
TTTGAGCTCT GCCAGCTGCT GCCCCTTTTT CTGCCCCCAA CAACTGTGCC CCCTGAATGC 
CACCGAGATC TGCAGGCTGG GCTGGCACGA ATCCTAGGAA GCAAGTTGAG CTCCTGGCAG 
CGCAACCCTG CACTGAAGCT GGCAGCCCGC CTGGCTCATG CCTGCGGCTC CGACTGGATC 
CCAGTGGGCA GCTCTGGGAG CAAGTTTCTG GCCCTGCTCG TGAATCTAGC GTGCGTGGAG 
GTACGGCTGG CTCTCGAGGA GACAGGCACA GAGGTGAAAG AAGACGTGGT AACAGCCTGC 
TATGCCCTTA TGGAATTGGG GATCCAGGAG TGCACCCGCT GTGAGCAGTC CCTGCTTAAG 
GAGCCACAGA AGGTTCAGCT CGTGAGCATT ATGAAAGAGG CCATTGGCGC TGTCATCCAC 
TACCTGCTGC AGGTGGGGCC AGAGAAGCAG AAAGAGCCCT TTGTGTTTGC CTCGGTGCGG 
ATCCTGGGTG CCTGGCTGGC GGAGGAGACC TCATCCCTGC GTAAGGAGGT GTGCCAACTG 
CTGCCCTTCC TTGTCCGATA TGCCAAGACA CTCTATGAGG AGGCTGAGGA GGCCAGTGAC 
ATTTCGCAGC AGGTGGCCAA CTTGGCCATC TCTCCTACTA CACCAGGGCC TTCATGGCCA 
GGGGATGCTC TCCGGCTCCT CCTTCCCGGC TGGTGTCACC TGACTGTTGA AGATGGTCCC 
CGGGAGATTC TGATCAAGGA AGGAGCCCCC TCACTTCTGT GCAAGTACTT CCTGCAGCAG 
TGGGAACTCA CATCCCCGGG CCATGATACC TCGGTGCTGC CAGACAGCGT GGAGATCGGC 
CTTCAGACCT GTTGCCACAT CTTCCTCAAC CTGGTGGTCA CCGCTCCAGG GCTGATCAAA 
CGCGATGCCT GCTTCACATC TCTTATGAAC ACCCTGATGA CGTCACTGCC CTCACTAGTG 
CAGCAACAAG GGAGACTGCT TCTAGCTGCC AACGTGGCCA CTTTGGGGCT CCTAATGGCC 
CGGCTCCTTA GCACCTCTCC AGCTCTCCAA GGAACCCCAG CCTCCCGAGG TTTCTTCGCA 
GCTGCCATCC TCTTTCTGTC ACAGTCCCAT GTGGCACGAG CCACCCCTGG CTCTGACCAG 
GCAGTGTTGG CCCTGTCCCC TGACTATGAA GGCATCTGGG CTGACTTGCA AGAGCTCTGG 
TTCCTGGGCA TGCAGGCCTT CACGGGTTGT GTGCCGCTGC TGCCCTGGCT GGCCCCTGCC 
GCCCTGCGCT CCCGCTGGCC ACAGGAGCTG CTACAACTGC TAGGTAGTGT AAGCCCCAAC 
TCCGTCAAGC CTGAGATGGT GGCTGCCTAC CAGGGCGTGC TGGTGGAATT GGCACGGGCT 
AACCGGCTAT GCCGGGAGGC CATGAGGCTG CAGGCGGGTG AAGAAACGGC CAGCCATTAC 
CGAATGGCTG CTTTGGAGCA GTGCCTGTCA GAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342342.1
CDS	70..2208
Translation	MASDCEPALNQAESRNPTLERYLGALREAKNDSEQFAALLLVTKAVKAGDIDAKTRRRIFDAVGFTFPNRLLTTKEAPDGCPDHVLRALGVALLACFCSDPELASHPQVLNKIPILSTFLTARGDPDDAARRSMIDDTYQCLTAVAGTPRGPRHLIAGGTVSALCQAYLGHGYGFDQALALLVGLLAAAETQCWKEAEPDLLAVLRGLSEDFQRAEDASKFELCQLLPLFLPPTTVPPECHRDLQAGLARILGSKLSSWQRNPALKLAARLAHACGSDWIPVGSSGSKFLALLVNLACVEVRLALEETGTEVKEDVVTACYALMELGIQECTRCEQSLLKEPQKVQLVSIMKEAIGAVIHYLLQVGPEKQKEPFVFASVRILGAWLAEETSSLRKEVCQLLPFLVRYAKTLYEEAEEASDISQQVANLAISPTTPGPSWPGDALRLLLPGWCHLTVEDGPREILIKEGAPSLLCKYFLQQWELTSPGHDTSVLPDSVEIGLQTCCHIFLNLVVTAPGLIKRDACFTSLMNTLMTSLPSLVQQQGRLLLAANVATLGLLMARLLSTSPALQGTPASRGFFAAAILFLSQSHVARATPGSDQAVLALSPDYEGIWADLQELWFLGMQAFTGCVPLLPWLAPAALRSRWPQELLQLLGSVSPNSVKPEMVAAYQGVLVELARANRLCREAMRLQAGEETASHYRMAALEQCLSEP

Target ORF information:

RefSeq Version	NM_001355413.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurochondrin (Ncdn), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355413.1

ORF Insert Sequence:

ATGGCCTCGG ATTGTGAGCC AGCTCTGAAC CAGGCAGAGA GCCGAAACCC CACCCTGGAG 
CGCTACCTGG GAGCCCTCCG TGAGGCCAAG AATGACAGCG AGCAGTTTGC AGCCCTGCTG 
CTAGTAACGA AGGCAGTCAA AGCAGGTGAC ATTGATGCCA AAACCCGACG TAGGATCTTT 
GATGCCGTTG GGTTCACCTT TCCCAACCGA CTCCTGACCA CTAAGGAGGC ACCTGATGGC 
TGCCCTGACC ACGTTCTCCG GGCCCTGGGC GTGGCCCTGC TGGCCTGTTT CTGCAGCGAC 
CCTGAACTAG CCAGCCATCC CCAGGTCCTG AACAAGATCC CCATCCTTAG CACATTCCTT 
ACAGCCCGGG GGGACCCTGA TGATGCTGCC CGCCGCTCCA TGATCGATGA CACCTACCAG 
TGCCTGACAG CTGTTGCGGG CACACCCCGA GGGCCCCGGC ACCTCATTGC TGGTGGCACC 
GTGTCTGCCC TGTGCCAGGC GTACCTGGGG CATGGCTACG GCTTTGACCA GGCTCTGGCA 
CTCTTGGTGG GGCTGCTGGC TGCTGCAGAG ACACAGTGCT GGAAGGAGGC GGAGCCCGAC 
CTGCTGGCTG TGTTGCGAGG CCTCAGTGAG GATTTCCAAA GAGCCGAAGA TGCCAGCAAG 
TTTGAGCTCT GCCAGCTGCT GCCCCTTTTT CTGCCCCCAA CAACTGTGCC CCCTGAATGC 
CACCGAGATC TGCAGGCTGG GCTGGCACGA ATCCTAGGAA GCAAGTTGAG CTCCTGGCAG 
CGCAACCCTG CACTGAAGCT GGCAGCCCGC CTGGCTCATG CCTGCGGCTC CGACTGGATC 
CCAGTGGGCA GCTCTGGGAG CAAGTTTCTG GCCCTGCTCG TGAATCTAGC GTGCGTGGAG 
GTACGGCTGG CTCTCGAGGA GACAGGCACA GAGGTGAAAG AAGACGTGGT AACAGCCTGC 
TATGCCCTTA TGGAATTGGG GATCCAGGAG TGCACCCGCT GTGAGCAGTC CCTGCTTAAG 
GAGCCACAGA AGGTTCAGCT CGTGAGCATT ATGAAAGAGG CCATTGGCGC TGTCATCCAC 
TACCTGCTGC AGGTGGGGCC AGAGAAGCAG AAAGAGCCCT TTGTGTTTGC CTCGGTGCGG 
ATCCTGGGTG CCTGGCTGGC GGAGGAGACC TCATCCCTGC GTAAGGAGGT GTGCCAACTG 
CTGCCCTTCC TTGTCCGATA TGCCAAGACA CTCTATGAGG AGGCTGAGGA GGCCAGTGAC 
ATTTCGCAGC AGGTGGCCAA CTTGGCCATC TCTCCTACTA CACCAGGGCC TTCATGGCCA 
GGGGATGCTC TCCGGCTCCT CCTTCCCGGC TGGTGTCACC TGACTGTTGA AGATGGTCCC 
CGGGAGATTC TGATCAAGGA AGGAGCCCCC TCACTTCTGT GCAAGTACTT CCTGCAGCAG 
TGGGAACTCA CATCCCCGGG CCATGATACC TCGGTGCTGC CAGACAGCGT GGAGATCGGC 
CTTCAGACCT GTTGCCACAT CTTCCTCAAC CTGGTGGTCA CCGCTCCAGG GCTGATCAAA 
CGCGATGCCT GCTTCACATC TCTTATGAAC ACCCTGATGA CGTCACTGCC CTCACTAGTG 
CAGCAACAAG GGAGACTGCT TCTAGCTGCC AACGTGGCCA CTTTGGGGCT CCTAATGGCC 
CGGCTCCTTA GCACCTCTCC AGCTCTCCAA GGAACCCCAG CCTCCCGAGG TTTCTTCGCA 
GCTGCCATCC TCTTTCTGTC ACAGTCCCAT GTGGCACGAG CCACCCCTGG CTCTGACCAG 
GCAGTGTTGG CCCTGTCCCC TGACTATGAA GGCATCTGGG CTGACTTGCA AGAGCTCTGG 
TTCCTGGGCA TGCAGGCCTT CACGGGTTGT GTGCCGCTGC TGCCCTGGCT GGCCCCTGCC 
GCCCTGCGCT CCCGCTGGCC ACAGGAGCTG CTACAACTGC TAGGTAGTGT AAGCCCCAAC 
TCCGTCAAGC CTGAGATGGT GGCTGCCTAC CAGGGCGTGC TGGTGGAATT GGCACGGGCT 
AACCGGCTAT GCCGGGAGGC CATGAGGCTG CAGGCGGGTG AAGAAACGGC CAGCCATTAC 
CGAATGGCTG CTTTGGAGCA GTGCCTGTCA GAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncdn ( NM_001355412.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001342341.1 Mus musculus neurochondrin (Ncdn), transcript variant 2, mRNA.

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CloneID	OMu33605
Clone ID Related Accession (Same CDS sequence)	XM_006503119.3 , XM_006503118.3 , NM_001355413.1 , NM_001355412.1
Accession Version	NM_001355412.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	neurochondrin isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY248917.1, AL606908.10, CJ142935.1, CD548169.1, BQ745150.1, CX568560.1, AK002938.1 and BE985399.1. On Sep 20, 2017 this sequence version replaced XM_006503118.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.85748.1, SRR1660815.100265.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGG ATTGTGAGCC AGCTCTGAAC CAGGCAGAGA GCCGAAACCC CACCCTGGAG 
CGCTACCTGG GAGCCCTCCG TGAGGCCAAG AATGACAGCG AGCAGTTTGC AGCCCTGCTG 
CTAGTAACGA AGGCAGTCAA AGCAGGTGAC ATTGATGCCA AAACCCGACG TAGGATCTTT 
GATGCCGTTG GGTTCACCTT TCCCAACCGA CTCCTGACCA CTAAGGAGGC ACCTGATGGC 
TGCCCTGACC ACGTTCTCCG GGCCCTGGGC GTGGCCCTGC TGGCCTGTTT CTGCAGCGAC 
CCTGAACTAG CCAGCCATCC CCAGGTCCTG AACAAGATCC CCATCCTTAG CACATTCCTT 
ACAGCCCGGG GGGACCCTGA TGATGCTGCC CGCCGCTCCA TGATCGATGA CACCTACCAG 
TGCCTGACAG CTGTTGCGGG CACACCCCGA GGGCCCCGGC ACCTCATTGC TGGTGGCACC 
GTGTCTGCCC TGTGCCAGGC GTACCTGGGG CATGGCTACG GCTTTGACCA GGCTCTGGCA 
CTCTTGGTGG GGCTGCTGGC TGCTGCAGAG ACACAGTGCT GGAAGGAGGC GGAGCCCGAC 
CTGCTGGCTG TGTTGCGAGG CCTCAGTGAG GATTTCCAAA GAGCCGAAGA TGCCAGCAAG 
TTTGAGCTCT GCCAGCTGCT GCCCCTTTTT CTGCCCCCAA CAACTGTGCC CCCTGAATGC 
CACCGAGATC TGCAGGCTGG GCTGGCACGA ATCCTAGGAA GCAAGTTGAG CTCCTGGCAG 
CGCAACCCTG CACTGAAGCT GGCAGCCCGC CTGGCTCATG CCTGCGGCTC CGACTGGATC 
CCAGTGGGCA GCTCTGGGAG CAAGTTTCTG GCCCTGCTCG TGAATCTAGC GTGCGTGGAG 
GTACGGCTGG CTCTCGAGGA GACAGGCACA GAGGTGAAAG AAGACGTGGT AACAGCCTGC 
TATGCCCTTA TGGAATTGGG GATCCAGGAG TGCACCCGCT GTGAGCAGTC CCTGCTTAAG 
GAGCCACAGA AGGTTCAGCT CGTGAGCATT ATGAAAGAGG CCATTGGCGC TGTCATCCAC 
TACCTGCTGC AGGTGGGGCC AGAGAAGCAG AAAGAGCCCT TTGTGTTTGC CTCGGTGCGG 
ATCCTGGGTG CCTGGCTGGC GGAGGAGACC TCATCCCTGC GTAAGGAGGT GTGCCAACTG 
CTGCCCTTCC TTGTCCGATA TGCCAAGACA CTCTATGAGG AGGCTGAGGA GGCCAGTGAC 
ATTTCGCAGC AGGTGGCCAA CTTGGCCATC TCTCCTACTA CACCAGGGCC TTCATGGCCA 
GGGGATGCTC TCCGGCTCCT CCTTCCCGGC TGGTGTCACC TGACTGTTGA AGATGGTCCC 
CGGGAGATTC TGATCAAGGA AGGAGCCCCC TCACTTCTGT GCAAGTACTT CCTGCAGCAG 
TGGGAACTCA CATCCCCGGG CCATGATACC TCGGTGCTGC CAGACAGCGT GGAGATCGGC 
CTTCAGACCT GTTGCCACAT CTTCCTCAAC CTGGTGGTCA CCGCTCCAGG GCTGATCAAA 
CGCGATGCCT GCTTCACATC TCTTATGAAC ACCCTGATGA CGTCACTGCC CTCACTAGTG 
CAGCAACAAG GGAGACTGCT TCTAGCTGCC AACGTGGCCA CTTTGGGGCT CCTAATGGCC 
CGGCTCCTTA GCACCTCTCC AGCTCTCCAA GGAACCCCAG CCTCCCGAGG TTTCTTCGCA 
GCTGCCATCC TCTTTCTGTC ACAGTCCCAT GTGGCACGAG CCACCCCTGG CTCTGACCAG 
GCAGTGTTGG CCCTGTCCCC TGACTATGAA GGCATCTGGG CTGACTTGCA AGAGCTCTGG 
TTCCTGGGCA TGCAGGCCTT CACGGGTTGT GTGCCGCTGC TGCCCTGGCT GGCCCCTGCC 
GCCCTGCGCT CCCGCTGGCC ACAGGAGCTG CTACAACTGC TAGGTAGTGT AAGCCCCAAC 
TCCGTCAAGC CTGAGATGGT GGCTGCCTAC CAGGGCGTGC TGGTGGAATT GGCACGGGCT 
AACCGGCTAT GCCGGGAGGC CATGAGGCTG CAGGCGGGTG AAGAAACGGC CAGCCATTAC 
CGAATGGCTG CTTTGGAGCA GTGCCTGTCA GAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342341.1
CDS	460..2598
Translation	MASDCEPALNQAESRNPTLERYLGALREAKNDSEQFAALLLVTKAVKAGDIDAKTRRRIFDAVGFTFPNRLLTTKEAPDGCPDHVLRALGVALLACFCSDPELASHPQVLNKIPILSTFLTARGDPDDAARRSMIDDTYQCLTAVAGTPRGPRHLIAGGTVSALCQAYLGHGYGFDQALALLVGLLAAAETQCWKEAEPDLLAVLRGLSEDFQRAEDASKFELCQLLPLFLPPTTVPPECHRDLQAGLARILGSKLSSWQRNPALKLAARLAHACGSDWIPVGSSGSKFLALLVNLACVEVRLALEETGTEVKEDVVTACYALMELGIQECTRCEQSLLKEPQKVQLVSIMKEAIGAVIHYLLQVGPEKQKEPFVFASVRILGAWLAEETSSLRKEVCQLLPFLVRYAKTLYEEAEEASDISQQVANLAISPTTPGPSWPGDALRLLLPGWCHLTVEDGPREILIKEGAPSLLCKYFLQQWELTSPGHDTSVLPDSVEIGLQTCCHIFLNLVVTAPGLIKRDACFTSLMNTLMTSLPSLVQQQGRLLLAANVATLGLLMARLLSTSPALQGTPASRGFFAAAILFLSQSHVARATPGSDQAVLALSPDYEGIWADLQELWFLGMQAFTGCVPLLPWLAPAALRSRWPQELLQLLGSVSPNSVKPEMVAAYQGVLVELARANRLCREAMRLQAGEETASHYRMAALEQCLSEP

Target ORF information:

RefSeq Version	NM_001355412.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurochondrin (Ncdn), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355412.1

ORF Insert Sequence:

ATGGCCTCGG ATTGTGAGCC AGCTCTGAAC CAGGCAGAGA GCCGAAACCC CACCCTGGAG 
CGCTACCTGG GAGCCCTCCG TGAGGCCAAG AATGACAGCG AGCAGTTTGC AGCCCTGCTG 
CTAGTAACGA AGGCAGTCAA AGCAGGTGAC ATTGATGCCA AAACCCGACG TAGGATCTTT 
GATGCCGTTG GGTTCACCTT TCCCAACCGA CTCCTGACCA CTAAGGAGGC ACCTGATGGC 
TGCCCTGACC ACGTTCTCCG GGCCCTGGGC GTGGCCCTGC TGGCCTGTTT CTGCAGCGAC 
CCTGAACTAG CCAGCCATCC CCAGGTCCTG AACAAGATCC CCATCCTTAG CACATTCCTT 
ACAGCCCGGG GGGACCCTGA TGATGCTGCC CGCCGCTCCA TGATCGATGA CACCTACCAG 
TGCCTGACAG CTGTTGCGGG CACACCCCGA GGGCCCCGGC ACCTCATTGC TGGTGGCACC 
GTGTCTGCCC TGTGCCAGGC GTACCTGGGG CATGGCTACG GCTTTGACCA GGCTCTGGCA 
CTCTTGGTGG GGCTGCTGGC TGCTGCAGAG ACACAGTGCT GGAAGGAGGC GGAGCCCGAC 
CTGCTGGCTG TGTTGCGAGG CCTCAGTGAG GATTTCCAAA GAGCCGAAGA TGCCAGCAAG 
TTTGAGCTCT GCCAGCTGCT GCCCCTTTTT CTGCCCCCAA CAACTGTGCC CCCTGAATGC 
CACCGAGATC TGCAGGCTGG GCTGGCACGA ATCCTAGGAA GCAAGTTGAG CTCCTGGCAG 
CGCAACCCTG CACTGAAGCT GGCAGCCCGC CTGGCTCATG CCTGCGGCTC CGACTGGATC 
CCAGTGGGCA GCTCTGGGAG CAAGTTTCTG GCCCTGCTCG TGAATCTAGC GTGCGTGGAG 
GTACGGCTGG CTCTCGAGGA GACAGGCACA GAGGTGAAAG AAGACGTGGT AACAGCCTGC 
TATGCCCTTA TGGAATTGGG GATCCAGGAG TGCACCCGCT GTGAGCAGTC CCTGCTTAAG 
GAGCCACAGA AGGTTCAGCT CGTGAGCATT ATGAAAGAGG CCATTGGCGC TGTCATCCAC 
TACCTGCTGC AGGTGGGGCC AGAGAAGCAG AAAGAGCCCT TTGTGTTTGC CTCGGTGCGG 
ATCCTGGGTG CCTGGCTGGC GGAGGAGACC TCATCCCTGC GTAAGGAGGT GTGCCAACTG 
CTGCCCTTCC TTGTCCGATA TGCCAAGACA CTCTATGAGG AGGCTGAGGA GGCCAGTGAC 
ATTTCGCAGC AGGTGGCCAA CTTGGCCATC TCTCCTACTA CACCAGGGCC TTCATGGCCA 
GGGGATGCTC TCCGGCTCCT CCTTCCCGGC TGGTGTCACC TGACTGTTGA AGATGGTCCC 
CGGGAGATTC TGATCAAGGA AGGAGCCCCC TCACTTCTGT GCAAGTACTT CCTGCAGCAG 
TGGGAACTCA CATCCCCGGG CCATGATACC TCGGTGCTGC CAGACAGCGT GGAGATCGGC 
CTTCAGACCT GTTGCCACAT CTTCCTCAAC CTGGTGGTCA CCGCTCCAGG GCTGATCAAA 
CGCGATGCCT GCTTCACATC TCTTATGAAC ACCCTGATGA CGTCACTGCC CTCACTAGTG 
CAGCAACAAG GGAGACTGCT TCTAGCTGCC AACGTGGCCA CTTTGGGGCT CCTAATGGCC 
CGGCTCCTTA GCACCTCTCC AGCTCTCCAA GGAACCCCAG CCTCCCGAGG TTTCTTCGCA 
GCTGCCATCC TCTTTCTGTC ACAGTCCCAT GTGGCACGAG CCACCCCTGG CTCTGACCAG 
GCAGTGTTGG CCCTGTCCCC TGACTATGAA GGCATCTGGG CTGACTTGCA AGAGCTCTGG 
TTCCTGGGCA TGCAGGCCTT CACGGGTTGT GTGCCGCTGC TGCCCTGGCT GGCCCCTGCC 
GCCCTGCGCT CCCGCTGGCC ACAGGAGCTG CTACAACTGC TAGGTAGTGT AAGCCCCAAC 
TCCGTCAAGC CTGAGATGGT GGCTGCCTAC CAGGGCGTGC TGGTGGAATT GGCACGGGCT 
AACCGGCTAT GCCGGGAGGC CATGAGGCTG CAGGCGGGTG AAGAAACGGC CAGCCATTAC 
CGAATGGCTG CTTTGGAGCA GTGCCTGTCA GAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncdn ( XM_006503119.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503182.1 Mus musculus neurochondrin (Ncdn), transcript variant X2, mRNA.

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CloneID	OMu33605
Clone ID Related Accession (Same CDS sequence)	XM_006503119.3 , XM_006503118.3 , NM_001355413.1 , NM_001355412.1
Accession Version	XM_006503119.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	neurochondrin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006503119.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGG ATTGTGAGCC AGCTCTGAAC CAGGCAGAGA GCCGAAACCC CACCCTGGAG 
CGCTACCTGG GAGCCCTCCG TGAGGCCAAG AATGACAGCG AGCAGTTTGC AGCCCTGCTG 
CTAGTAACGA AGGCAGTCAA AGCAGGTGAC ATTGATGCCA AAACCCGACG TAGGATCTTT 
GATGCCGTTG GGTTCACCTT TCCCAACCGA CTCCTGACCA CTAAGGAGGC ACCTGATGGC 
TGCCCTGACC ACGTTCTCCG GGCCCTGGGC GTGGCCCTGC TGGCCTGTTT CTGCAGCGAC 
CCTGAACTAG CCAGCCATCC CCAGGTCCTG AACAAGATCC CCATCCTTAG CACATTCCTT 
ACAGCCCGGG GGGACCCTGA TGATGCTGCC CGCCGCTCCA TGATCGATGA CACCTACCAG 
TGCCTGACAG CTGTTGCGGG CACACCCCGA GGGCCCCGGC ACCTCATTGC TGGTGGCACC 
GTGTCTGCCC TGTGCCAGGC GTACCTGGGG CATGGCTACG GCTTTGACCA GGCTCTGGCA 
CTCTTGGTGG GGCTGCTGGC TGCTGCAGAG ACACAGTGCT GGAAGGAGGC GGAGCCCGAC 
CTGCTGGCTG TGTTGCGAGG CCTCAGTGAG GATTTCCAAA GAGCCGAAGA TGCCAGCAAG 
TTTGAGCTCT GCCAGCTGCT GCCCCTTTTT CTGCCCCCAA CAACTGTGCC CCCTGAATGC 
CACCGAGATC TGCAGGCTGG GCTGGCACGA ATCCTAGGAA GCAAGTTGAG CTCCTGGCAG 
CGCAACCCTG CACTGAAGCT GGCAGCCCGC CTGGCTCATG CCTGCGGCTC CGACTGGATC 
CCAGTGGGCA GCTCTGGGAG CAAGTTTCTG GCCCTGCTCG TGAATCTAGC GTGCGTGGAG 
GTACGGCTGG CTCTCGAGGA GACAGGCACA GAGGTGAAAG AAGACGTGGT AACAGCCTGC 
TATGCCCTTA TGGAATTGGG GATCCAGGAG TGCACCCGCT GTGAGCAGTC CCTGCTTAAG 
GAGCCACAGA AGGTTCAGCT CGTGAGCATT ATGAAAGAGG CCATTGGCGC TGTCATCCAC 
TACCTGCTGC AGGTGGGGCC AGAGAAGCAG AAAGAGCCCT TTGTGTTTGC CTCGGTGCGG 
ATCCTGGGTG CCTGGCTGGC GGAGGAGACC TCATCCCTGC GTAAGGAGGT GTGCCAACTG 
CTGCCCTTCC TTGTCCGATA TGCCAAGACA CTCTATGAGG AGGCTGAGGA GGCCAGTGAC 
ATTTCGCAGC AGGTGGCCAA CTTGGCCATC TCTCCTACTA CACCAGGGCC TTCATGGCCA 
GGGGATGCTC TCCGGCTCCT CCTTCCCGGC TGGTGTCACC TGACTGTTGA AGATGGTCCC 
CGGGAGATTC TGATCAAGGA AGGAGCCCCC TCACTTCTGT GCAAGTACTT CCTGCAGCAG 
TGGGAACTCA CATCCCCGGG CCATGATACC TCGGTGCTGC CAGACAGCGT GGAGATCGGC 
CTTCAGACCT GTTGCCACAT CTTCCTCAAC CTGGTGGTCA CCGCTCCAGG GCTGATCAAA 
CGCGATGCCT GCTTCACATC TCTTATGAAC ACCCTGATGA CGTCACTGCC CTCACTAGTG 
CAGCAACAAG GGAGACTGCT TCTAGCTGCC AACGTGGCCA CTTTGGGGCT CCTAATGGCC 
CGGCTCCTTA GCACCTCTCC AGCTCTCCAA GGAACCCCAG CCTCCCGAGG TTTCTTCGCA 
GCTGCCATCC TCTTTCTGTC ACAGTCCCAT GTGGCACGAG CCACCCCTGG CTCTGACCAG 
GCAGTGTTGG CCCTGTCCCC TGACTATGAA GGCATCTGGG CTGACTTGCA AGAGCTCTGG 
TTCCTGGGCA TGCAGGCCTT CACGGGTTGT GTGCCGCTGC TGCCCTGGCT GGCCCCTGCC 
GCCCTGCGCT CCCGCTGGCC ACAGGAGCTG CTACAACTGC TAGGTAGTGT AAGCCCCAAC 
TCCGTCAAGC CTGAGATGGT GGCTGCCTAC CAGGGCGTGC TGGTGGAATT GGCACGGGCT 
AACCGGCTAT GCCGGGAGGC CATGAGGCTG CAGGCGGGTG AAGAAACGGC CAGCCATTAC 
CGAATGGCTG CTTTGGAGCA GTGCCTGTCA GAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503182.1
CDS	465..2603
Translation	MASDCEPALNQAESRNPTLERYLGALREAKNDSEQFAALLLVTKAVKAGDIDAKTRRRIFDAVGFTFPNRLLTTKEAPDGCPDHVLRALGVALLACFCSDPELASHPQVLNKIPILSTFLTARGDPDDAARRSMIDDTYQCLTAVAGTPRGPRHLIAGGTVSALCQAYLGHGYGFDQALALLVGLLAAAETQCWKEAEPDLLAVLRGLSEDFQRAEDASKFELCQLLPLFLPPTTVPPECHRDLQAGLARILGSKLSSWQRNPALKLAARLAHACGSDWIPVGSSGSKFLALLVNLACVEVRLALEETGTEVKEDVVTACYALMELGIQECTRCEQSLLKEPQKVQLVSIMKEAIGAVIHYLLQVGPEKQKEPFVFASVRILGAWLAEETSSLRKEVCQLLPFLVRYAKTLYEEAEEASDISQQVANLAISPTTPGPSWPGDALRLLLPGWCHLTVEDGPREILIKEGAPSLLCKYFLQQWELTSPGHDTSVLPDSVEIGLQTCCHIFLNLVVTAPGLIKRDACFTSLMNTLMTSLPSLVQQQGRLLLAANVATLGLLMARLLSTSPALQGTPASRGFFAAAILFLSQSHVARATPGSDQAVLALSPDYEGIWADLQELWFLGMQAFTGCVPLLPWLAPAALRSRWPQELLQLLGSVSPNSVKPEMVAAYQGVLVELARANRLCREAMRLQAGEETASHYRMAALEQCLSEP

Target ORF information:

RefSeq Version	XM_006503119.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus neurochondrin (Ncdn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503119.3

ORF Insert Sequence:

ATGGCCTCGG ATTGTGAGCC AGCTCTGAAC CAGGCAGAGA GCCGAAACCC CACCCTGGAG 
CGCTACCTGG GAGCCCTCCG TGAGGCCAAG AATGACAGCG AGCAGTTTGC AGCCCTGCTG 
CTAGTAACGA AGGCAGTCAA AGCAGGTGAC ATTGATGCCA AAACCCGACG TAGGATCTTT 
GATGCCGTTG GGTTCACCTT TCCCAACCGA CTCCTGACCA CTAAGGAGGC ACCTGATGGC 
TGCCCTGACC ACGTTCTCCG GGCCCTGGGC GTGGCCCTGC TGGCCTGTTT CTGCAGCGAC 
CCTGAACTAG CCAGCCATCC CCAGGTCCTG AACAAGATCC CCATCCTTAG CACATTCCTT 
ACAGCCCGGG GGGACCCTGA TGATGCTGCC CGCCGCTCCA TGATCGATGA CACCTACCAG 
TGCCTGACAG CTGTTGCGGG CACACCCCGA GGGCCCCGGC ACCTCATTGC TGGTGGCACC 
GTGTCTGCCC TGTGCCAGGC GTACCTGGGG CATGGCTACG GCTTTGACCA GGCTCTGGCA 
CTCTTGGTGG GGCTGCTGGC TGCTGCAGAG ACACAGTGCT GGAAGGAGGC GGAGCCCGAC 
CTGCTGGCTG TGTTGCGAGG CCTCAGTGAG GATTTCCAAA GAGCCGAAGA TGCCAGCAAG 
TTTGAGCTCT GCCAGCTGCT GCCCCTTTTT CTGCCCCCAA CAACTGTGCC CCCTGAATGC 
CACCGAGATC TGCAGGCTGG GCTGGCACGA ATCCTAGGAA GCAAGTTGAG CTCCTGGCAG 
CGCAACCCTG CACTGAAGCT GGCAGCCCGC CTGGCTCATG CCTGCGGCTC CGACTGGATC 
CCAGTGGGCA GCTCTGGGAG CAAGTTTCTG GCCCTGCTCG TGAATCTAGC GTGCGTGGAG 
GTACGGCTGG CTCTCGAGGA GACAGGCACA GAGGTGAAAG AAGACGTGGT AACAGCCTGC 
TATGCCCTTA TGGAATTGGG GATCCAGGAG TGCACCCGCT GTGAGCAGTC CCTGCTTAAG 
GAGCCACAGA AGGTTCAGCT CGTGAGCATT ATGAAAGAGG CCATTGGCGC TGTCATCCAC 
TACCTGCTGC AGGTGGGGCC AGAGAAGCAG AAAGAGCCCT TTGTGTTTGC CTCGGTGCGG 
ATCCTGGGTG CCTGGCTGGC GGAGGAGACC TCATCCCTGC GTAAGGAGGT GTGCCAACTG 
CTGCCCTTCC TTGTCCGATA TGCCAAGACA CTCTATGAGG AGGCTGAGGA GGCCAGTGAC 
ATTTCGCAGC AGGTGGCCAA CTTGGCCATC TCTCCTACTA CACCAGGGCC TTCATGGCCA 
GGGGATGCTC TCCGGCTCCT CCTTCCCGGC TGGTGTCACC TGACTGTTGA AGATGGTCCC 
CGGGAGATTC TGATCAAGGA AGGAGCCCCC TCACTTCTGT GCAAGTACTT CCTGCAGCAG 
TGGGAACTCA CATCCCCGGG CCATGATACC TCGGTGCTGC CAGACAGCGT GGAGATCGGC 
CTTCAGACCT GTTGCCACAT CTTCCTCAAC CTGGTGGTCA CCGCTCCAGG GCTGATCAAA 
CGCGATGCCT GCTTCACATC TCTTATGAAC ACCCTGATGA CGTCACTGCC CTCACTAGTG 
CAGCAACAAG GGAGACTGCT TCTAGCTGCC AACGTGGCCA CTTTGGGGCT CCTAATGGCC 
CGGCTCCTTA GCACCTCTCC AGCTCTCCAA GGAACCCCAG CCTCCCGAGG TTTCTTCGCA 
GCTGCCATCC TCTTTCTGTC ACAGTCCCAT GTGGCACGAG CCACCCCTGG CTCTGACCAG 
GCAGTGTTGG CCCTGTCCCC TGACTATGAA GGCATCTGGG CTGACTTGCA AGAGCTCTGG 
TTCCTGGGCA TGCAGGCCTT CACGGGTTGT GTGCCGCTGC TGCCCTGGCT GGCCCCTGCC 
GCCCTGCGCT CCCGCTGGCC ACAGGAGCTG CTACAACTGC TAGGTAGTGT AAGCCCCAAC 
TCCGTCAAGC CTGAGATGGT GGCTGCCTAC CAGGGCGTGC TGGTGGAATT GGCACGGGCT 
AACCGGCTAT GCCGGGAGGC CATGAGGCTG CAGGCGGGTG AAGAAACGGC CAGCCATTAC 
CGAATGGCTG CTTTGGAGCA GTGCCTGTCA GAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncdn ( XM_006503118.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503181.1 Mus musculus neurochondrin (Ncdn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu33605
Clone ID Related Accession (Same CDS sequence)	XM_006503119.3 , XM_006503118.3 , NM_001355413.1 , NM_001355412.1
Accession Version	XM_006503118.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	neurochondrin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006503118.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGG ATTGTGAGCC AGCTCTGAAC CAGGCAGAGA GCCGAAACCC CACCCTGGAG 
CGCTACCTGG GAGCCCTCCG TGAGGCCAAG AATGACAGCG AGCAGTTTGC AGCCCTGCTG 
CTAGTAACGA AGGCAGTCAA AGCAGGTGAC ATTGATGCCA AAACCCGACG TAGGATCTTT 
GATGCCGTTG GGTTCACCTT TCCCAACCGA CTCCTGACCA CTAAGGAGGC ACCTGATGGC 
TGCCCTGACC ACGTTCTCCG GGCCCTGGGC GTGGCCCTGC TGGCCTGTTT CTGCAGCGAC 
CCTGAACTAG CCAGCCATCC CCAGGTCCTG AACAAGATCC CCATCCTTAG CACATTCCTT 
ACAGCCCGGG GGGACCCTGA TGATGCTGCC CGCCGCTCCA TGATCGATGA CACCTACCAG 
TGCCTGACAG CTGTTGCGGG CACACCCCGA GGGCCCCGGC ACCTCATTGC TGGTGGCACC 
GTGTCTGCCC TGTGCCAGGC GTACCTGGGG CATGGCTACG GCTTTGACCA GGCTCTGGCA 
CTCTTGGTGG GGCTGCTGGC TGCTGCAGAG ACACAGTGCT GGAAGGAGGC GGAGCCCGAC 
CTGCTGGCTG TGTTGCGAGG CCTCAGTGAG GATTTCCAAA GAGCCGAAGA TGCCAGCAAG 
TTTGAGCTCT GCCAGCTGCT GCCCCTTTTT CTGCCCCCAA CAACTGTGCC CCCTGAATGC 
CACCGAGATC TGCAGGCTGG GCTGGCACGA ATCCTAGGAA GCAAGTTGAG CTCCTGGCAG 
CGCAACCCTG CACTGAAGCT GGCAGCCCGC CTGGCTCATG CCTGCGGCTC CGACTGGATC 
CCAGTGGGCA GCTCTGGGAG CAAGTTTCTG GCCCTGCTCG TGAATCTAGC GTGCGTGGAG 
GTACGGCTGG CTCTCGAGGA GACAGGCACA GAGGTGAAAG AAGACGTGGT AACAGCCTGC 
TATGCCCTTA TGGAATTGGG GATCCAGGAG TGCACCCGCT GTGAGCAGTC CCTGCTTAAG 
GAGCCACAGA AGGTTCAGCT CGTGAGCATT ATGAAAGAGG CCATTGGCGC TGTCATCCAC 
TACCTGCTGC AGGTGGGGCC AGAGAAGCAG AAAGAGCCCT TTGTGTTTGC CTCGGTGCGG 
ATCCTGGGTG CCTGGCTGGC GGAGGAGACC TCATCCCTGC GTAAGGAGGT GTGCCAACTG 
CTGCCCTTCC TTGTCCGATA TGCCAAGACA CTCTATGAGG AGGCTGAGGA GGCCAGTGAC 
ATTTCGCAGC AGGTGGCCAA CTTGGCCATC TCTCCTACTA CACCAGGGCC TTCATGGCCA 
GGGGATGCTC TCCGGCTCCT CCTTCCCGGC TGGTGTCACC TGACTGTTGA AGATGGTCCC 
CGGGAGATTC TGATCAAGGA AGGAGCCCCC TCACTTCTGT GCAAGTACTT CCTGCAGCAG 
TGGGAACTCA CATCCCCGGG CCATGATACC TCGGTGCTGC CAGACAGCGT GGAGATCGGC 
CTTCAGACCT GTTGCCACAT CTTCCTCAAC CTGGTGGTCA CCGCTCCAGG GCTGATCAAA 
CGCGATGCCT GCTTCACATC TCTTATGAAC ACCCTGATGA CGTCACTGCC CTCACTAGTG 
CAGCAACAAG GGAGACTGCT TCTAGCTGCC AACGTGGCCA CTTTGGGGCT CCTAATGGCC 
CGGCTCCTTA GCACCTCTCC AGCTCTCCAA GGAACCCCAG CCTCCCGAGG TTTCTTCGCA 
GCTGCCATCC TCTTTCTGTC ACAGTCCCAT GTGGCACGAG CCACCCCTGG CTCTGACCAG 
GCAGTGTTGG CCCTGTCCCC TGACTATGAA GGCATCTGGG CTGACTTGCA AGAGCTCTGG 
TTCCTGGGCA TGCAGGCCTT CACGGGTTGT GTGCCGCTGC TGCCCTGGCT GGCCCCTGCC 
GCCCTGCGCT CCCGCTGGCC ACAGGAGCTG CTACAACTGC TAGGTAGTGT AAGCCCCAAC 
TCCGTCAAGC CTGAGATGGT GGCTGCCTAC CAGGGCGTGC TGGTGGAATT GGCACGGGCT 
AACCGGCTAT GCCGGGAGGC CATGAGGCTG CAGGCGGGTG AAGAAACGGC CAGCCATTAC 
CGAATGGCTG CTTTGGAGCA GTGCCTGTCA GAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503181.1
CDS	332..2470
Translation	MASDCEPALNQAESRNPTLERYLGALREAKNDSEQFAALLLVTKAVKAGDIDAKTRRRIFDAVGFTFPNRLLTTKEAPDGCPDHVLRALGVALLACFCSDPELASHPQVLNKIPILSTFLTARGDPDDAARRSMIDDTYQCLTAVAGTPRGPRHLIAGGTVSALCQAYLGHGYGFDQALALLVGLLAAAETQCWKEAEPDLLAVLRGLSEDFQRAEDASKFELCQLLPLFLPPTTVPPECHRDLQAGLARILGSKLSSWQRNPALKLAARLAHACGSDWIPVGSSGSKFLALLVNLACVEVRLALEETGTEVKEDVVTACYALMELGIQECTRCEQSLLKEPQKVQLVSIMKEAIGAVIHYLLQVGPEKQKEPFVFASVRILGAWLAEETSSLRKEVCQLLPFLVRYAKTLYEEAEEASDISQQVANLAISPTTPGPSWPGDALRLLLPGWCHLTVEDGPREILIKEGAPSLLCKYFLQQWELTSPGHDTSVLPDSVEIGLQTCCHIFLNLVVTAPGLIKRDACFTSLMNTLMTSLPSLVQQQGRLLLAANVATLGLLMARLLSTSPALQGTPASRGFFAAAILFLSQSHVARATPGSDQAVLALSPDYEGIWADLQELWFLGMQAFTGCVPLLPWLAPAALRSRWPQELLQLLGSVSPNSVKPEMVAAYQGVLVELARANRLCREAMRLQAGEETASHYRMAALEQCLSEP

Target ORF information:

RefSeq Version	XM_006503118.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus neurochondrin (Ncdn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503118.3

ORF Insert Sequence:

ATGGCCTCGG ATTGTGAGCC AGCTCTGAAC CAGGCAGAGA GCCGAAACCC CACCCTGGAG 
CGCTACCTGG GAGCCCTCCG TGAGGCCAAG AATGACAGCG AGCAGTTTGC AGCCCTGCTG 
CTAGTAACGA AGGCAGTCAA AGCAGGTGAC ATTGATGCCA AAACCCGACG TAGGATCTTT 
GATGCCGTTG GGTTCACCTT TCCCAACCGA CTCCTGACCA CTAAGGAGGC ACCTGATGGC 
TGCCCTGACC ACGTTCTCCG GGCCCTGGGC GTGGCCCTGC TGGCCTGTTT CTGCAGCGAC 
CCTGAACTAG CCAGCCATCC CCAGGTCCTG AACAAGATCC CCATCCTTAG CACATTCCTT 
ACAGCCCGGG GGGACCCTGA TGATGCTGCC CGCCGCTCCA TGATCGATGA CACCTACCAG 
TGCCTGACAG CTGTTGCGGG CACACCCCGA GGGCCCCGGC ACCTCATTGC TGGTGGCACC 
GTGTCTGCCC TGTGCCAGGC GTACCTGGGG CATGGCTACG GCTTTGACCA GGCTCTGGCA 
CTCTTGGTGG GGCTGCTGGC TGCTGCAGAG ACACAGTGCT GGAAGGAGGC GGAGCCCGAC 
CTGCTGGCTG TGTTGCGAGG CCTCAGTGAG GATTTCCAAA GAGCCGAAGA TGCCAGCAAG 
TTTGAGCTCT GCCAGCTGCT GCCCCTTTTT CTGCCCCCAA CAACTGTGCC CCCTGAATGC 
CACCGAGATC TGCAGGCTGG GCTGGCACGA ATCCTAGGAA GCAAGTTGAG CTCCTGGCAG 
CGCAACCCTG CACTGAAGCT GGCAGCCCGC CTGGCTCATG CCTGCGGCTC CGACTGGATC 
CCAGTGGGCA GCTCTGGGAG CAAGTTTCTG GCCCTGCTCG TGAATCTAGC GTGCGTGGAG 
GTACGGCTGG CTCTCGAGGA GACAGGCACA GAGGTGAAAG AAGACGTGGT AACAGCCTGC 
TATGCCCTTA TGGAATTGGG GATCCAGGAG TGCACCCGCT GTGAGCAGTC CCTGCTTAAG 
GAGCCACAGA AGGTTCAGCT CGTGAGCATT ATGAAAGAGG CCATTGGCGC TGTCATCCAC 
TACCTGCTGC AGGTGGGGCC AGAGAAGCAG AAAGAGCCCT TTGTGTTTGC CTCGGTGCGG 
ATCCTGGGTG CCTGGCTGGC GGAGGAGACC TCATCCCTGC GTAAGGAGGT GTGCCAACTG 
CTGCCCTTCC TTGTCCGATA TGCCAAGACA CTCTATGAGG AGGCTGAGGA GGCCAGTGAC 
ATTTCGCAGC AGGTGGCCAA CTTGGCCATC TCTCCTACTA CACCAGGGCC TTCATGGCCA 
GGGGATGCTC TCCGGCTCCT CCTTCCCGGC TGGTGTCACC TGACTGTTGA AGATGGTCCC 
CGGGAGATTC TGATCAAGGA AGGAGCCCCC TCACTTCTGT GCAAGTACTT CCTGCAGCAG 
TGGGAACTCA CATCCCCGGG CCATGATACC TCGGTGCTGC CAGACAGCGT GGAGATCGGC 
CTTCAGACCT GTTGCCACAT CTTCCTCAAC CTGGTGGTCA CCGCTCCAGG GCTGATCAAA 
CGCGATGCCT GCTTCACATC TCTTATGAAC ACCCTGATGA CGTCACTGCC CTCACTAGTG 
CAGCAACAAG GGAGACTGCT TCTAGCTGCC AACGTGGCCA CTTTGGGGCT CCTAATGGCC 
CGGCTCCTTA GCACCTCTCC AGCTCTCCAA GGAACCCCAG CCTCCCGAGG TTTCTTCGCA 
GCTGCCATCC TCTTTCTGTC ACAGTCCCAT GTGGCACGAG CCACCCCTGG CTCTGACCAG 
GCAGTGTTGG CCCTGTCCCC TGACTATGAA GGCATCTGGG CTGACTTGCA AGAGCTCTGG 
TTCCTGGGCA TGCAGGCCTT CACGGGTTGT GTGCCGCTGC TGCCCTGGCT GGCCCCTGCC 
GCCCTGCGCT CCCGCTGGCC ACAGGAGCTG CTACAACTGC TAGGTAGTGT AAGCCCCAAC 
TCCGTCAAGC CTGAGATGGT GGCTGCCTAC CAGGGCGTGC TGGTGGAATT GGCACGGGCT 
AACCGGCTAT GCCGGGAGGC CATGAGGCTG CAGGCGGGTG AAGAAACGGC CAGCCATTAC 
CGAATGGCTG CTTTGGAGCA GTGCCTGTCA GAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncdn ( XM_030253580.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109440.1 Mus musculus neurochondrin (Ncdn), transcript variant X1, mRNA.

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CloneID	OMu88544
Clone ID Related Accession (Same CDS sequence)	XM_030253580.1
Accession Version	XM_030253580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurochondrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGATG ACACCTACCA GTGCCTGACA GCTGTTGCGG GCACACCCCG AGGGCCCCGG 
CACCTCATTG CTGGTGGCAC CGTGTCTGCC CTGTGCCAGG CGTACCTGGG GCATGGCTAC 
GGCTTTGACC AGGCTCTGGC ACTCTTGGTG GGGCTGCTGG CTGCTGCAGA GACACAGTGC 
TGGAAGGAGG CGGAGCCCGA CCTGCTGGCT GTGTTGCGAG GCCTCAGTGA GGATTTCCAA 
AGAGCCGAAG ATGCCAGCAA GTTTGAGCTC TGCCAGCTGC TGCCCCTTTT TCTGCCCCCA 
ACAACTGTGC CCCCTGAATG CCACCGAGAT CTGCAGGCTG GGCTGGCACG AATCCTAGGA 
AGCAAGTTGA GCTCCTGGCA GCGCAACCCT GCACTGAAGC TGGCAGCCCG CCTGGCTCAT 
GCCTGCGGCT CCGACTGGAT CCCAGTGGGC AGCTCTGGGA GCAAGTTTCT GGCCCTGCTC 
GTGAATCTAG CGTGCGTGGA GGTACGGCTG GCTCTCGAGG AGACAGGCAC AGAGGTGAAA 
GAAGACGTGG TAACAGCCTG CTATGCCCTT ATGGAATTGG GGATCCAGGA GTGCACCCGC 
TGTGAGCAGT CCCTGCTTAA GGAGCCACAG AAGGTTCAGC TCGTGAGCAT TATGAAAGAG 
GCCATTGGCG CTGTCATCCA CTACCTGCTG CAGGTGGGGC CAGAGAAGCA GAAAGAGCCC 
TTTGTGTTTG CCTCGGTGCG GATCCTGGGT GCCTGGCTGG CGGAGGAGAC CTCATCCCTG 
CGTAAGGAGG TGTGCCAACT GCTGCCCTTC CTTGTCCGAT ATGCCAAGAC ACTCTATGAG 
GAGGCTGAGG AGGCCAGTGA CATTTCGCAG CAGGTGGCCA ACTTGGCCAT CTCTCCTACT 
ACACCAGGGC CTTCATGGCC AGGGGATGCT CTCCGGCTCC TCCTTCCCGG CTGGTGTCAC 
CTGACTGTTG AAGATGGTCC CCGGGAGATT CTGATCAAGG AAGGAGCCCC CTCACTTCTG 
TGCAAGTACT TCCTGCAGCA GTGGGAACTC ACATCCCCGG GCCATGATAC CTCGGTGCTG 
CCAGACAGCG TGGAGATCGG CCTTCAGACC TGTTGCCACA TCTTCCTCAA CCTGGTGGTC 
ACCGCTCCAG GGCTGATCAA ACGCGATGCC TGCTTCACAT CTCTTATGAA CACCCTGATG 
ACGTCACTGC CCTCACTAGT GCAGCAACAA GGGAGACTGC TTCTAGCTGC CAACGTGGCC 
ACTTTGGGGC TCCTAATGGC CCGGCTCCTT AGCACCTCTC CAGCTCTCCA AGGAACCCCA 
GCCTCCCGAG GTTTCTTCGC AGCTGCCATC CTCTTTCTGT CACAGTCCCA TGTGGCACGA 
GCCACCCCTG GCTCTGACCA GGCAGTGTTG GCCCTGTCCC CTGACTATGA AGGCATCTGG 
GCTGACTTGC AAGAGCTCTG GTTCCTGGGC ATGCAGGCCT TCACGGGTTG TGTGCCGCTG 
CTGCCCTGGC TGGCCCCTGC CGCCCTGCGC TCCCGCTGGC CACAGGAGCT GCTACAACTG 
CTAGGTAGTG TAAGCCCCAA CTCCGTCAAG CCTGAGATGG TGGCTGCCTA CCAGGGCGTG 
CTGGTGGAAT TGGCACGGGC TAACCGGCTA TGCCGGGAGG CCATGAGGCT GCAGGCGGGT 
GAAGAAACGG CCAGCCATTA CCGAATGGCT GCTTTGGAGC AGTGCCTGTC AGAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109440.1
CDS	710..2449
Translation	MIDDTYQCLTAVAGTPRGPRHLIAGGTVSALCQAYLGHGYGFDQALALLVGLLAAAETQCWKEAEPDLLAVLRGLSEDFQRAEDASKFELCQLLPLFLPPTTVPPECHRDLQAGLARILGSKLSSWQRNPALKLAARLAHACGSDWIPVGSSGSKFLALLVNLACVEVRLALEETGTEVKEDVVTACYALMELGIQECTRCEQSLLKEPQKVQLVSIMKEAIGAVIHYLLQVGPEKQKEPFVFASVRILGAWLAEETSSLRKEVCQLLPFLVRYAKTLYEEAEEASDISQQVANLAISPTTPGPSWPGDALRLLLPGWCHLTVEDGPREILIKEGAPSLLCKYFLQQWELTSPGHDTSVLPDSVEIGLQTCCHIFLNLVVTAPGLIKRDACFTSLMNTLMTSLPSLVQQQGRLLLAANVATLGLLMARLLSTSPALQGTPASRGFFAAAILFLSQSHVARATPGSDQAVLALSPDYEGIWADLQELWFLGMQAFTGCVPLLPWLAPAALRSRWPQELLQLLGSVSPNSVKPEMVAAYQGVLVELARANRLCREAMRLQAGEETASHYRMAALEQCLSEP

Target ORF information:

RefSeq Version	XM_030253580.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neurochondrin (Ncdn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253580.1

ORF Insert Sequence:

ATGATCGATG ACACCTACCA GTGCCTGACA GCTGTTGCGG GCACACCCCG AGGGCCCCGG 
CACCTCATTG CTGGTGGCAC CGTGTCTGCC CTGTGCCAGG CGTACCTGGG GCATGGCTAC 
GGCTTTGACC AGGCTCTGGC ACTCTTGGTG GGGCTGCTGG CTGCTGCAGA GACACAGTGC 
TGGAAGGAGG CGGAGCCCGA CCTGCTGGCT GTGTTGCGAG GCCTCAGTGA GGATTTCCAA 
AGAGCCGAAG ATGCCAGCAA GTTTGAGCTC TGCCAGCTGC TGCCCCTTTT TCTGCCCCCA 
ACAACTGTGC CCCCTGAATG CCACCGAGAT CTGCAGGCTG GGCTGGCACG AATCCTAGGA 
AGCAAGTTGA GCTCCTGGCA GCGCAACCCT GCACTGAAGC TGGCAGCCCG CCTGGCTCAT 
GCCTGCGGCT CCGACTGGAT CCCAGTGGGC AGCTCTGGGA GCAAGTTTCT GGCCCTGCTC 
GTGAATCTAG CGTGCGTGGA GGTACGGCTG GCTCTCGAGG AGACAGGCAC AGAGGTGAAA 
GAAGACGTGG TAACAGCCTG CTATGCCCTT ATGGAATTGG GGATCCAGGA GTGCACCCGC 
TGTGAGCAGT CCCTGCTTAA GGAGCCACAG AAGGTTCAGC TCGTGAGCAT TATGAAAGAG 
GCCATTGGCG CTGTCATCCA CTACCTGCTG CAGGTGGGGC CAGAGAAGCA GAAAGAGCCC 
TTTGTGTTTG CCTCGGTGCG GATCCTGGGT GCCTGGCTGG CGGAGGAGAC CTCATCCCTG 
CGTAAGGAGG TGTGCCAACT GCTGCCCTTC CTTGTCCGAT ATGCCAAGAC ACTCTATGAG 
GAGGCTGAGG AGGCCAGTGA CATTTCGCAG CAGGTGGCCA ACTTGGCCAT CTCTCCTACT 
ACACCAGGGC CTTCATGGCC AGGGGATGCT CTCCGGCTCC TCCTTCCCGG CTGGTGTCAC 
CTGACTGTTG AAGATGGTCC CCGGGAGATT CTGATCAAGG AAGGAGCCCC CTCACTTCTG 
TGCAAGTACT TCCTGCAGCA GTGGGAACTC ACATCCCCGG GCCATGATAC CTCGGTGCTG 
CCAGACAGCG TGGAGATCGG CCTTCAGACC TGTTGCCACA TCTTCCTCAA CCTGGTGGTC 
ACCGCTCCAG GGCTGATCAA ACGCGATGCC TGCTTCACAT CTCTTATGAA CACCCTGATG 
ACGTCACTGC CCTCACTAGT GCAGCAACAA GGGAGACTGC TTCTAGCTGC CAACGTGGCC 
ACTTTGGGGC TCCTAATGGC CCGGCTCCTT AGCACCTCTC CAGCTCTCCA AGGAACCCCA 
GCCTCCCGAG GTTTCTTCGC AGCTGCCATC CTCTTTCTGT CACAGTCCCA TGTGGCACGA 
GCCACCCCTG GCTCTGACCA GGCAGTGTTG GCCCTGTCCC CTGACTATGA AGGCATCTGG 
GCTGACTTGC AAGAGCTCTG GTTCCTGGGC ATGCAGGCCT TCACGGGTTG TGTGCCGCTG 
CTGCCCTGGC TGGCCCCTGC CGCCCTGCGC TCCCGCTGGC CACAGGAGCT GCTACAACTG 
CTAGGTAGTG TAAGCCCCAA CTCCGTCAAG CCTGAGATGG TGGCTGCCTA CCAGGGCGTG 
CTGGTGGAAT TGGCACGGGC TAACCGGCTA TGCCGGGAGG CCATGAGGCT GCAGGCGGGT 
GAAGAAACGG CCAGCCATTA CCGAATGGCT GCTTTGGAGC AGTGCCTGTC AGAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Norbin Stimulates the Catalytic Activity and Plasma Membrane Localization of the Guanine-Nucleotide Exchange Factor P-Rex1.
The Journal of biological chemistry291(12)6359-75(2016 Mar)
Pan D,Barber MA,Hornigold K,Baker MJ,Toth JM,Oxley D,Welch HC

Norbin ablation results in defective adult hippocampal neurogenesis and depressive-like behavior in mice.
Proceedings of the National Academy of Sciences of the United States of America112(31)9745-50(2015 Aug)
Wang H,Warner-Schmidt J,Varela S,Enikolopov G,Greengard P,Flajolet M

In silico screening for palmitoyl substrates reveals a role for DHHC1/3/10 (zDHHC1/3/11)-mediated neurochondrin palmitoylation in its targeting to Rab5-positive endosomes.
The Journal of biological chemistry288(27)19816-29(2013 Jul)
Oku S,Takahashi N,Fukata Y,Fukata M

Norbin is an endogenous regulator of metabotropic glutamate receptor 5 signaling.
Science (New York, N.Y.)326(5959)1554-7(2009 Dec)
Wang H,Westin L,Nong Y,Birnbaum S,Bendor J,Brismar H,Nestler E,Aperia A,Flajolet M,Greengard P

The level of the transcription factor Pax6 is essential for controlling the balance between neural stem cell self-renewal and neurogenesis.
PLoS genetics5(6)e1000511(2009 Jun)
Sansom SN,Griffiths DS,Faedo A,Kleinjan DJ,Ruan Y,Smith J,van Heyningen V,Rubenstein JL,Livesey FJ

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Ncdn cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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