Bsx cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bsx
Entrez Gene ID	244813
Full Name	brain specific homeobox
Synonyms	Bsx1a,Bsx1b
General protein information	Preferred Names brain specific homeobox Names brain-specific homeobox protein homolog bsx
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 A5.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_178245.3	NP_839976.1	brain-specific homeobox protein homolog
XM_006510306.1	XP_006510369.1	brain-specific homeobox protein homolog isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	BSX	NP_989843.1
Xenopus tropicalis (tropical clawed frog)	bsx	NP_001095280.1
Bos taurus (cattle)	BSX	NP_001179998.1
Rattus norvegicus (Norway rat)	Bsx	NP_001178924.1
Danio rerio (zebrafish)	bsx	NP_999892.1
Canis lupus familiaris (dog)	BSX	XP_546472.2
Pan troglodytes (chimpanzee)	BSX	XP_522208.3
	BSX	XP_001107998.2
Homo sapiens (human)	BSX	NP_001091639.1
Mus musculus (house mouse)	Bsx	NP_839976.1

Gene Ontology
Bibliography

Related articles in PubMed

The LIM-homeobox transcription factor Isl1 plays crucial roles in the development of multiple arcuate nucleus neurons.
Lee B, Lee S, Lee SK, Lee JW
Development (Cambridge, England)143(20)3763-3773(2016 10)

Molecular and behavioral profiling of Dbx1-derived neurons in the arcuate, lateral and ventromedial hypothalamic nuclei.
Sokolowski K, Tran T, Esumi S, Kamal Y, Oboti L, Lischinsky J, Goodrich M, Lam A, Carter M, Nakagawa Y, Corbin JG
Neural development11(1)12(2016 05)

Chronic Activation of ?2 AMPK Induces Obesity and Reduces ? Cell Function.
Yavari A, Stocker CJ, Ghaffari S, Wargent ET, Steeples V, Czibik G, Pinter K, Bellahcene M, Woods A, Mart?nez de Morentin PB, Cansell C, Lam BY, Chuster A, Petkevicius K, Nguyen-Tu MS, Martinez-Sanchez A, Pullen TJ, Oliver PL, Stockenhuber A, Nguyen C, Lazdam M, O'Dowd JF, Harikumar P, T?th M, Beall C, Kyriakou T, Parnis J, Sarma D, Katritsis G, Wortmann DD, Harper AR, Brown LA, Willows R, Gandra S, Poncio V, de Oliveira Figueiredo MJ, Qi NR, Peirson SN, McCrimmon RJ, Gereben B, Tretter L, Fekete C, Redwood C, Yeo GS, Heisler LK, Rutter GA, Smith MA, Withers DJ, Carling D, Sternick EB, Arch JR, Cawthorne MA, Watkins H, Ashrafian H
Cell metabolism23(5)821-36(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Agouti related peptide is a common orexigenic target gene of glucocorticoid receptor and Bsx in the mouse hypothalamus.
Title: Brain-specific homeobox factor as a target selector for glucocorticoid receptor in energy balance.

Bsx and TTF-1 are transcriptional factors of NPY and AgRP in the arcuate nucleus[review]
Title: Role of homeobox genes in the hypothalamic development and energy balance.

binding of REST to neuron restrictive silencer element suppresses transcriptional activator Sp1-mediated activation of bsx in non-neuronal cells
Title: REST is a key regulator in brain-specific homeobox gene expression during neuronal differentiation.

These results demonstrate that Bsx is required for postnatal growth and maintenance of lactating mammary glands.
Title: A brain-specific homeobox gene, Bsx, is essential for proper postnatal growth and nursing.

Data demonstrate that BSX1A is a DNA binding protein and a transcriptional activator, and that BSX1A and BSX1B localize in the nuclei and cytoplasm, respectively.
Title: Cloning and functional analysis of hypothalamic homeobox gene Bsx1a and its isoform, Bsx1b.

The following Bsx gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bsx cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu07133	NM_178245.3 Latest version!	Mus musculus brain specific homeobox (Bsx), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu42205	XM_006510306.1 Latest version!	Mus musculus brain specific homeobox (Bsx), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bsx ( NM_178245.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_839976.1 Mus musculus brain specific homeobox (Bsx), mRNA.

Price & Availability↑

CloneID	OMu07133
Clone ID Related Accession (Same CDS sequence)	NM_178245.3
Accession Version	NM_178245.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Mus musculus(house mouse)
Product	brain-specific homeobox protein homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC158355.2. On Nov 17, 2006 this sequence version replaced NM_178245.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY233396.1, BC104386.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849378, SAMN01164137 [ECO:0000348] ##Evidence-Data-END##

ATGAATCTCA ACTTCACTTC CCCTCTTCAT CCAGCATCTT CTCAGAGGCC CACGTCGTTT 
TTCATCGAGG ACATTCTGCT ACACAAGCCC AAGCCGTTGA GGGAAGTGGC CCCTGACCAC 
TTTGCCAGCT CTCTGGCCTC TAGGGTGCCT TTGCTAGACT ATGGCTACCC CCTTATGCCC 
ACACCCACCC TCCTCACCCC TCACGCTCAT CATCCTCTGC ATAAGGGAGA TCACCACCAT 
CCTTATTTCC TGACCACCTC AGGGATGCCG GTCCCGGCGC TGTTCCCGCA CCCGCAGCAC 
GCGGAGTTGC CCGGGAAGCA CTGCCGCCGC CGCAAAGCTC GCACGGTGTT TTCTGACTCG 
CAGCTCTCCG GCCTGGAGAA AAGGTTCGAA ATCCAGCGCT ACCTGTCGAC GCCAGAGCGT 
GTGGAGCTGG CCACTGCCCT CAGCCTCTCA GAGACTCAGG TGAAAACGTG GTTCCAGAAC 
CGGCGGATGA AGCATAAAAA GCAGCTGAGG AAAAGCCAAG ACGAACCGAA AGCGGCAGAC 
GGGCCGGAGA GCCCCGAAGG CAGCCCTCGT GCTCCCGAGG GCGCGCCCGC CGACGCTCGG 
CTGAGCCTGC CCGCCGGTGC CTTCGTGCTT ACTGAGCCCG AGGACGAAGT GGACATTGGG 
GATGAAGGCG AGCTCAGCTC AGGGCCGCAT GTGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_839976.1
CDS	52..750
Translation	MNLNFTSPLHPASSQRPTSFFIEDILLHKPKPLREVAPDHFASSLASRVPLLDYGYPLMPTPTLLTPHAHHPLHKGDHHHPYFLTTSGMPVPALFPHPQHAELPGKHCRRRKARTVFSDSQLSGLEKRFEIQRYLSTPERVELATALSLSETQVKTWFQNRRMKHKKQLRKSQDEPKAADGPESPEGSPRAPEGAPADARLSLPAGAFVLTEPEDEVDIGDEGELSSGPHVL

Target ORF information:

RefSeq Version	NM_178245.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus brain specific homeobox (Bsx), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178245.3

ORF Insert Sequence:

ATGAATCTCA ACTTCACTTC CCCTCTTCAT CCAGCATCTT CTCAGAGGCC CACGTCGTTT 
TTCATCGAGG ACATTCTGCT ACACAAGCCC AAGCCGTTGA GGGAAGTGGC CCCTGACCAC 
TTTGCCAGCT CTCTGGCCTC TAGGGTGCCT TTGCTAGACT ATGGCTACCC CCTTATGCCC 
ACACCCACCC TCCTCACCCC TCACGCTCAT CATCCTCTGC ATAAGGGAGA TCACCACCAT 
CCTTATTTCC TGACCACCTC AGGGATGCCG GTCCCGGCGC TGTTCCCGCA CCCGCAGCAC 
GCGGAGTTGC CCGGGAAGCA CTGCCGCCGC CGCAAAGCTC GCACGGTGTT TTCTGACTCG 
CAGCTCTCCG GCCTGGAGAA AAGGTTCGAA ATCCAGCGCT ACCTGTCGAC GCCAGAGCGT 
GTGGAGCTGG CCACTGCCCT CAGCCTCTCA GAGACTCAGG TGAAAACGTG GTTCCAGAAC 
CGGCGGATGA AGCATAAAAA GCAGCTGAGG AAAAGCCAAG ACGAACCGAA AGCGGCAGAC 
GGGCCGGAGA GCCCCGAAGG CAGCCCTCGT GCTCCCGAGG GCGCGCCCGC CGACGCTCGG 
CTGAGCCTGC CCGCCGGTGC CTTCGTGCTT ACTGAGCCCG AGGACGAAGT GGACATTGGG 
GATGAAGGCG AGCTCAGCTC AGGGCCGCAT GTGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bsx ( XM_006510306.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510369.1 Mus musculus brain specific homeobox (Bsx), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu42205
Clone ID Related Accession (Same CDS sequence)	XM_006510306.1
Accession Version	XM_006510306.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	brain-specific homeobox protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGGG CGCCTAATAT ACCAGGGATG CCGGTCCCGG CGCTGTTCCC GCACCCGCAG 
CACGCGGAGT TGCCCGGGAA GCACTGCCGC CGCCGCAAAG CTCGCACGGT GTTTTCTGAC 
TCGCAGCTCT CCGGCCTGGA GAAAAGGTTC GAAATCCAGC GCTACCTGTC GACGCCAGAG 
CGTGTGGAGC TGGCCACTGC CCTCAGCCTC TCAGAGACTC AGGTGAAAAC GTGGTTCCAG 
AACCGGCGGA TGAAGCATAA AAAGCAGCTG AGGAAAAGCC AAGACGAACC GAAAGCGGCA 
GACGGGCCGG AGAGCCCCGA AGGCAGCCCT CGTGCTCCCG AGGGCGCGCC CGCCGACGCT 
CGGCTGAGCC TGCCCGCCGG TGCCTTCGTG CTTACTGAGC CCGAGGACGA AGTGGACATT 
GGGGATGAAG GCGAGCTCAG CTCAGGGCCG CATGTGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510369.1
CDS	62..523
Translation	MLGAPNIPGMPVPALFPHPQHAELPGKHCRRRKARTVFSDSQLSGLEKRFEIQRYLSTPERVELATALSLSETQVKTWFQNRRMKHKKQLRKSQDEPKAADGPESPEGSPRAPEGAPADARLSLPAGAFVLTEPEDEVDIGDEGELSSGPHVL

Target ORF information:

RefSeq Version	XM_006510306.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus brain specific homeobox (Bsx), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510306.1

ORF Insert Sequence:

ATGCTTGGGG CGCCTAATAT ACCAGGGATG CCGGTCCCGG CGCTGTTCCC GCACCCGCAG 
CACGCGGAGT TGCCCGGGAA GCACTGCCGC CGCCGCAAAG CTCGCACGGT GTTTTCTGAC 
TCGCAGCTCT CCGGCCTGGA GAAAAGGTTC GAAATCCAGC GCTACCTGTC GACGCCAGAG 
CGTGTGGAGC TGGCCACTGC CCTCAGCCTC TCAGAGACTC AGGTGAAAAC GTGGTTCCAG 
AACCGGCGGA TGAAGCATAA AAAGCAGCTG AGGAAAAGCC AAGACGAACC GAAAGCGGCA 
GACGGGCCGG AGAGCCCCGA AGGCAGCCCT CGTGCTCCCG AGGGCGCGCC CGCCGACGCT 
CGGCTGAGCC TGCCCGCCGG TGCCTTCGTG CTTACTGAGC CCGAGGACGA AGTGGACATT 
GGGGATGAAG GCGAGCTCAG CTCAGGGCCG CATGTGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The LIM-homeobox transcription factor Isl1 plays crucial roles in the development of multiple arcuate nucleus neurons.
Development (Cambridge, England)143(20)3763-3773(2016 10)
Lee B,Lee S,Lee SK,Lee JW

Molecular and behavioral profiling of Dbx1-derived neurons in the arcuate, lateral and ventromedial hypothalamic nuclei.
Neural development11(1)12(2016 05)
Sokolowski K,Tran T,Esumi S,Kamal Y,Oboti L,Lischinsky J,Goodrich M,Lam A,Carter M,Nakagawa Y,Corbin JG

Chronic Activation of ?2 AMPK Induces Obesity and Reduces ? Cell Function.
Cell metabolism23(5)821-36(2016 May)
Yavari A,Stocker CJ,Ghaffari S,Wargent ET,Steeples V,Czibik G,Pinter K,Bellahcene M,Woods A,Mart?nez de Morentin PB,Cansell C,Lam BY,Chuster A,Petkevicius K,Nguyen-Tu MS,Martinez-Sanchez A,Pullen TJ,Oliver PL,Stockenhuber A,Nguyen C,Lazdam M,O'Dowd JF,Harikumar P,T?th M,Beall C,Kyriakou T,Parnis J,Sarma D,Katritsis G,Wortmann DD,Harper AR,Brown LA,Willows R,Gandra S,Poncio V,de Oliveira Figueiredo MJ,Qi NR,Peirson SN,McCrimmon RJ,Gereben B,Tretter L,Fekete C,Redwood C,Yeo GS,Heisler LK,Rutter GA,Smith MA,Withers DJ,Carling D,Sternick EB,Arch JR,Cawthorne MA,Watkins H,Ashrafian H

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Bsx cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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