Cldn19 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cldn19
Entrez Gene ID	242653
Full Name	claudin 19
General protein information	Preferred Names claudin 19 Names claudin-19
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D2.1
Summary	This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. siRNA knockdown of this gene in mice develops the FHHNC (familial hypomagnesemia with hypercalciuria and nephrocalcinosis) symptoms of chronic renal wasting of magnesium and calcium together with defective renal salt handling. The protein encoded by this gene interacts with another family member, Claudin 16, and their interaction is required for their assembly into tight junctions and for renal reabsorption of magnesium. This protein is a constituent of tight junctions in the Schwann cells of peripheral myelinated nerves and the gene deficiency affects the nerve conduction of peripheral myelinated fibers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2010].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001038590.1	NP_001033679.1	claudin-19 isoform 1
NM_153105.7	NP_694745.1	claudin-19 isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CLDN19	XP_003949437.1
Canis lupus familiaris (dog)	CLDN19	XP_848612.2
Bos taurus (cattle)	CLDN19	NP_001094554.1
Mus musculus (house mouse)	Cldn19	NP_001033679.1
Rattus norvegicus (Norway rat)	Cldn19	NP_001008514.1
Homo sapiens (human)	CLDN19	NP_683763.2
Gallus gallus (chicken)	LOC769245	XP_003642589.2
	CLDN19	XP_001087771.1
Danio rerio (zebrafish)	cldn19	NP_001017736.1
Xenopus tropicalis (tropical clawed frog)	cldn19	XP_002937080.1

Gene Ontology
Bibliography

Related articles in PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
Sundberg JP, Dadras SS, Silva KA, Kennedy VE, Garland G, Murray SA, Sundberg BA, Schofield PN, Pratt CH
PloS one12(7)e0180682(2017)

Tuning PAK Activity to Rescue Abnormal Myelin Permeability in HNPP.
Hu B, Arpag S, Zhang X, M?bius W, Werner H, Sosinsky G, Ellisman M, Zhang Y, Hamilton A, Chernoff J, Li J
PLoS genetics12(9)e1006290(2016 Sep)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Tight junctions. Structural insight into tight junction disassembly by Clostridium perfringens enterotoxin.
Saitoh Y, Suzuki H, Tani K, Nishikawa K, Irie K, Ogura Y, Tamura A, Tsukita S, Fujiyoshi Y
Science (New York, N.Y.)347(6223)775-8(2015 Feb)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study presents the structure of mammalian claudin-19 in complex with C-CPE at 3.7 A resolution.
Title: Tight junctions. Structural insight into tight junction disassembly by Clostridium perfringens enterotoxin.

Claudin-16 and claudin-19 interaction is required for their assembly into tight junctions and for renal reabsorption of magnesium.
Title: Claudin-16 and claudin-19 interaction is required for their assembly into tight junctions and for renal reabsorption of magnesium.

Sp1 site is crucial for Cldn19 gene expression in kidney cells.
Title: Sp1 site is crucial for the mouse claudin-19 gene expression in the kidney cells.

The following Cldn19 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cldn19 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00197	NM_001038590.1 Latest version!	Mus musculus claudin 19 (Cldn19), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu02302	NM_153105.7 Latest version!	Mus musculus claudin 19 (Cldn19), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cldn19 ( NM_001038590.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001033679.1 Mus musculus claudin 19 (Cldn19), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu00197
Clone ID Related Accession (Same CDS sequence)	NM_001038590.1
Accession Version	NM_001038590.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Mus musculus(house mouse)
Product	claudin-19 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF486651.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF486651.2, CK129653.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849382 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAACT CGGGCCTCCA GCTCCTGGGC TACTTCCTAG CCTTGGGCGG CTGGGTGGGC 
ATCATCGCCA GCACTGCCCT GCCACAGTGG AAGCAGTCTT CCTATGCAGG CGATGCCATC 
ATCACTGCCG TGGGCCTCTA CGAAGGGCTG TGGATGTCTT GCGCCTCTCA GAGCACCGGG 
CAGGTGCAAT GCAAACTCTA CGATTCACTC CTGGCCCTGG ACGGTCATAT CCAGTCAGCA 
CGAGCCCTGA TGGTCGTGGC TGTGCTCCTG GGCTTTGTGG CCATGGTGCT CAGTGTCGTG 
GGCATGAAGT GCACTCGGGT TGGAGACAGT AACCCCACTG CCAAGAGCCG TGTGGCCATC 
TCCGGAGGTG CTCTCTTCCT CTTGGCAGGT CTCTGTACTT TGACTGCTGT CTCCTGGTAT 
GCTACCCTGG TAACACAGGA ATTCTTCAAC CCCAGCACTC CTGTCAATGC CAGGTACGAA 
TTTGGCCCAG CTCTGTTCGT CGGCTGGGCC TCGGCCGGCC TGGCCATGCT GGGCGGTTCC 
TTTCTCTGCT GCACATGCCC AGAGCCGGAG AGGGCGAACA GCATCCCACA GCCCTATCGC 
TCTGGACCCT CAACTGCTGC CAGAGAACCT GTTGTTAAGT TGCCCGCCTC GGTCAAGGGC 
CCCCTGGGTG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033679.1
CDS	105..779
Translation	MANSGLQLLGYFLALGGWVGIIASTALPQWKQSSYAGDAIITAVGLYEGLWMSCASQSTGQVQCKLYDSLLALDGHIQSARALMVVAVLLGFVAMVLSVVGMKCTRVGDSNPTAKSRVAISGGALFLLAGLCTLTAVSWYATLVTQEFFNPSTPVNARYEFGPALFVGWASAGLAMLGGSFLCCTCPEPERANSIPQPYRSGPSTAAREPVVKLPASVKGPLGV

Target ORF information:

RefSeq Version	NM_001038590.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus claudin 19 (Cldn19), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001038590.1

ORF Insert Sequence:

ATGGCCAACT CGGGCCTCCA GCTCCTGGGC TACTTCCTAG CCTTGGGCGG CTGGGTGGGC 
ATCATCGCCA GCACTGCCCT GCCACAGTGG AAGCAGTCTT CCTATGCAGG CGATGCCATC 
ATCACTGCCG TGGGCCTCTA CGAAGGGCTG TGGATGTCTT GCGCCTCTCA GAGCACCGGG 
CAGGTGCAAT GCAAACTCTA CGATTCACTC CTGGCCCTGG ACGGTCATAT CCAGTCAGCA 
CGAGCCCTGA TGGTCGTGGC TGTGCTCCTG GGCTTTGTGG CCATGGTGCT CAGTGTCGTG 
GGCATGAAGT GCACTCGGGT TGGAGACAGT AACCCCACTG CCAAGAGCCG TGTGGCCATC 
TCCGGAGGTG CTCTCTTCCT CTTGGCAGGT CTCTGTACTT TGACTGCTGT CTCCTGGTAT 
GCTACCCTGG TAACACAGGA ATTCTTCAAC CCCAGCACTC CTGTCAATGC CAGGTACGAA 
TTTGGCCCAG CTCTGTTCGT CGGCTGGGCC TCGGCCGGCC TGGCCATGCT GGGCGGTTCC 
TTTCTCTGCT GCACATGCCC AGAGCCGGAG AGGGCGAACA GCATCCCACA GCCCTATCGC 
TCTGGACCCT CAACTGCTGC CAGAGAACCT GTTGTTAAGT TGCCCGCCTC GGTCAAGGGC 
CCCCTGGGTG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cldn19 ( NM_153105.7 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_694745.1 Mus musculus claudin 19 (Cldn19), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu02302
Clone ID Related Accession (Same CDS sequence)	NM_153105.7
Accession Version	NM_153105.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-20
Organism	Mus musculus(house mouse)
Product	claudin-19 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL606975.9 and BX470153.3. On Oct 23, 2007 this sequence version replaced NM_153105.6. Transcript Variant: This variant (2) has an additional segment in the last splice junction, as compared to variant 1. The transcript is longer but the resulting isoform (2) is shorter at the C-terminus, as compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK143945.1, AK032743.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAACT CGGGCCTCCA GCTCCTGGGC TACTTCCTAG CCTTGGGCGG CTGGGTGGGC 
ATCATCGCCA GCACTGCCCT GCCACAGTGG AAGCAGTCTT CCTATGCAGG CGATGCCATC 
ATCACTGCCG TGGGCCTCTA CGAAGGGCTG TGGATGTCTT GCGCCTCTCA GAGCACCGGG 
CAGGTGCAAT GCAAACTCTA CGATTCACTC CTGGCCCTGG ACGGTCATAT CCAGTCAGCA 
CGAGCCCTGA TGGTCGTGGC TGTGCTCCTG GGCTTTGTGG CCATGGTGCT CAGTGTCGTG 
GGCATGAAGT GCACTCGGGT TGGAGACAGT AACCCCACTG CCAAGAGCCG TGTGGCCATC 
TCCGGAGGTG CTCTCTTCCT CTTGGCAGGT CTCTGTACTT TGACTGCTGT CTCCTGGTAT 
GCTACCCTGG TAACACAGGA ATTCTTCAAC CCCAGCACTC CTGTCAATGC CAGGTACGAA 
TTTGGCCCAG CTCTGTTCGT CGGCTGGGCC TCGGCCGGCC TGGCCATGCT GGGCGGTTCC 
TTTCTCTGCT GCACATGCCC AGAGCCGGAG AGGGCGAACA GCATCCCACA GCCCTATCGC 
TCTGGACCCT CAACTGCTGC CAGAGAGTAC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694745.1
CDS	133..768
Translation	MANSGLQLLGYFLALGGWVGIIASTALPQWKQSSYAGDAIITAVGLYEGLWMSCASQSTGQVQCKLYDSLLALDGHIQSARALMVVAVLLGFVAMVLSVVGMKCTRVGDSNPTAKSRVAISGGALFLLAGLCTLTAVSWYATLVTQEFFNPSTPVNARYEFGPALFVGWASAGLAMLGGSFLCCTCPEPERANSIPQPYRSGPSTAAREYV

Target ORF information:

RefSeq Version	NM_153105.7
Organism	Mus musculus(house mouse)
Definition	Mus musculus claudin 19 (Cldn19), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153105.7

ORF Insert Sequence:

ATGGCCAACT CGGGCCTCCA GCTCCTGGGC TACTTCCTAG CCTTGGGCGG CTGGGTGGGC 
ATCATCGCCA GCACTGCCCT GCCACAGTGG AAGCAGTCTT CCTATGCAGG CGATGCCATC 
ATCACTGCCG TGGGCCTCTA CGAAGGGCTG TGGATGTCTT GCGCCTCTCA GAGCACCGGG 
CAGGTGCAAT GCAAACTCTA CGATTCACTC CTGGCCCTGG ACGGTCATAT CCAGTCAGCA 
CGAGCCCTGA TGGTCGTGGC TGTGCTCCTG GGCTTTGTGG CCATGGTGCT CAGTGTCGTG 
GGCATGAAGT GCACTCGGGT TGGAGACAGT AACCCCACTG CCAAGAGCCG TGTGGCCATC 
TCCGGAGGTG CTCTCTTCCT CTTGGCAGGT CTCTGTACTT TGACTGCTGT CTCCTGGTAT 
GCTACCCTGG TAACACAGGA ATTCTTCAAC CCCAGCACTC CTGTCAATGC CAGGTACGAA 
TTTGGCCCAG CTCTGTTCGT CGGCTGGGCC TCGGCCGGCC TGGCCATGCT GGGCGGTTCC 
TTTCTCTGCT GCACATGCCC AGAGCCGGAG AGGGCGAACA GCATCCCACA GCCCTATCGC 
TCTGGACCCT CAACTGCTGC CAGAGAGTAC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
PloS one12(7)e0180682(2017)
Sundberg JP,Dadras SS,Silva KA,Kennedy VE,Garland G,Murray SA,Sundberg BA,Schofield PN,Pratt CH

Tuning PAK Activity to Rescue Abnormal Myelin Permeability in HNPP.
PLoS genetics12(9)e1006290(2016 Sep)
Hu B,Arpag S,Zhang X,M?bius W,Werner H,Sosinsky G,Ellisman M,Zhang Y,Hamilton A,Chernoff J,Li J

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Tight junctions. Structural insight into tight junction disassembly by Clostridium perfringens enterotoxin.
Science (New York, N.Y.)347(6223)775-8(2015 Feb)
Saitoh Y,Suzuki H,Tani K,Nishikawa K,Irie K,Ogura Y,Tamura A,Tsukita S,Fujiyoshi Y

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

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Cldn19 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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