ADAMTS5 cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	ADAMTS5
Entrez Gene ID	23794
Full Name	a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2)
Synonyms	9530092O11Rik,ADAM-TS5,ADAMTS1,ADAMTS11,ADMP-2,AI481094,ASMP-2
General protein information	Preferred Names a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2) Names A disintegrin and metalloproteinase with thrombospondin motifs 5 aggrecanase-2 implantin
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 16 Map Location: 16 C3.3\|16 48.34 cM
Summary	This gene encodes a member of 'a disintegrin and metalloproteinase with thrombospondin motifs' (ADAMTS) family of multi-domain matrix-associated metalloendopeptidases that have diverse roles in tissue morphogenesis and pathophysiological remodeling, in inflammation and in vascular biology. The encoded preproprotein undergoes proteolytic processing to generate an active, zinc-dependent aggrecanase enzyme. Mice lacking the encoded protein are protected from surgery-induced osteoarthritis and antigen-induced arthritis. [provided by RefSeq, May 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_011782.2	NP_035912.2	A disintegrin and metalloproteinase with thrombospondin motifs 5 preproprotein
XM_011246127.1	XP_011244429.1	A disintegrin and metalloproteinase with thrombospondin motifs 5 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Adamts5	NP_035912.2
Canis lupus familiaris (dog)	ADAMTS5	XP_851118.2
Bos taurus (cattle)	ADAMTS5	NP_001159987.1
Gallus gallus (chicken)	ADAMTS5	XP_425541.4
Xenopus tropicalis (tropical clawed frog)	adamts5	XP_002935740.1
Pan troglodytes (chimpanzee)	ADAMTS5	XP_531407.2
Danio rerio (zebrafish)	adamts5	XP_693454.6
Homo sapiens (human)	ADAMTS5	NP_008969.2
	ADAMTS5	XP_001103439.1
Rattus norvegicus (Norway rat)	Adamts5	NP_942056.1

Gene Ontology

The following ADAMTS5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADAMTS5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08840	NM_011782.2 Latest version!	Mus musculus a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2) (Adamts5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu08840	XM_011246127.1 Latest version!	Mus musculus a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2) (Adamts5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ADAMTS5 ( NM_011782.2 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_035912.2 Mus musculus a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2) (Adamts5), mRNA.

Price & Availability↑

CloneID	OMu08840
Clone ID Related Accession (Same CDS sequence)	NM_011782.2 , XM_011246127.1
Accession Version	NM_011782.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 5 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC163107.3, AK044746.1 and AC126936.4. On Feb 9, 2007 this sequence version replaced NM_011782.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.213468.1, AF140673.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END##

ATGCGGCTCG AGTGGGCGCC CTTGTTGCTG CTACTGCTGC TGCTGAGCGC GTCCTGCCTG 
TCCCTGGCCG CTGACAGCCC CGCCGCGGCA CCTGCCCAGG ATAAAACCAG GCAGCCTCAG 
GCTGCAGCAG CGGCCGCCGA GCCGGACCAG CCGCAGGGGG AGGAAACACG GGAGCGAGGC 
CATTTACAAC CCTTGGCCGG GCAGCGCAGG AGCGGCGGGC TGGTCCAGAA TATAGACCAA 
CTCTACTCTG GCGGTGGCAA AGTGGGCTAC CTTGTCTACG CGGGCGGCCG GAGGTTCCTG 
CTGGACCTGG AGAGAGATGA CACAGTGGGT GCTGCTGGTA GCATCGTTAC TGCAGGAGGA 
GGGCTGAGCG CATCCTCTGG CCACCGGGGT CACTGTTTCT ACAGAGGCAC CGTGGACGGC 
AGCCCTCGAT CCCTAGCTGT CTTTGACCTC TGCGGGGGTC TCGATGGCTT CTTTGCAGTC 
AAGCATGCGC GCTACACTCT AAAGCCACTC CTGCGTGGGT CCTGGGCAGA GTATGAACGA 
ATTTATGGGG ATGGATCTTC CCGCATCCTG CATGTCTACA ACCGCGAGGG CTTTAGCTTC 
GAGGCCCTGC CGCCACGCGC CAGTTGCGAG ACTCCTGCAT CCCCATCTGG GCCCCAAGAG 
AGCCCCTCGG TGCACAGTAG ATCTAGGAGA CGCTCAGCGC TGGCCCCGCA GCTGCTGGAC 
CACTCAGCTT TCTCGCCATC TGGGAACGCG GGACCTCAGA CTTGGTGGAG GCGTAGGCGC 
CGTTCCATCT CCAGGGCCCG CCAGGTGGAG CTCCTCTTGG TGGCTGACTC GTCCATGGCC 
AGGATGTATG GGCGGGGCCT GCAGCATTAC CTGCTGACCC TGGCCTCCAT CGCCAACAGG 
CTGTACAGTC ATGCAAGCAT TGAGAACCAC ATCCGCCTGG CGGTGGTGAA GGTGGTGGTG 
CTGACGGACA AGGACACGAG TCTGGAGGTG AGCAAGAATG CGGCCACGAC CCTCAAGAAC 
TTTTGCAAAT GGCAGCACCA ACATAACCAG CTAGGGGATG ATCACGAAGA GCACTACGAT 
GCAGCCATCC TGTTCACCCG AGAGGATTTA TGTGGGCATC ATTCATGTGA CACCCTGGGA 
ATGGCAGACG TTGGGACCAT ATGTTCTCCG GAGCGCAGCT GTGCAGTGAT TGAAGATGAT 
GGCCTCCATG CAGCCTTCAC TGTGGCTCAT GAAATTGGGC ATCTACTTGG CCTTTCTCAT 
GACGATTCCA AATTCTGTGA AGAGAACTTC GGTACTACAG AAGACAAGCG TTTAATGTCT 
TCAATCCTTA CCAGCATCGA TGCATCCAAG CCCTGGTCCA AATGCACGTC AGCCACCATC 
ACAGAATTCC TGGATGATGG TCATGGTAAT TGTTTGCTAG ACCTACCACG GAAGCAGATT 
TTGGGTCCCG AGGAACTCCC AGGACAGACC TACGATGCCA CCCAGCAGTG CAACTTGACA 
TTTGGGCCTG AGTACTCGGT GTGCCCTGGC ATGGATGTCT GTGCGCGGCT GTGGTGTGCT 
GTGGTGCGCC AAGGCCAAAT GGTGTGTCTG ACCAAGAAGC TGCCGGCTGT GGAGGGCACT 
CCCTGTGGGA AGGGAAGAGT CTGCCTTCAA GGCAAATGTG TGGACAAAAC TAAGAAAAAA 
TATTACTCGA CATCAAGCCA TGGAAATTGG GGGTCCTGGG GCCCCTGGGG TCAGTGTTCT 
CGCTCATGCG GGGGAGGAGT GCAGTTTGCC TACCGCCATT GTAATAACCC TGCACCTCGA 
AACAGTGGCC GCTACTGCAC AGGGAAGAGG GCCATATACC GTTCCTGCAG TGTTACACCC 
TGCCCACCCA ATGGTAAATC TTTTCGCCAT GAGCAGTGTG AAGCCAAAAA TGGCTATCAG 
TCTGATGCAA AAGGAGTCAA AACATTTGTA GAATGGGTTC CCAAATATGC AGGTGTCCTG 
CCGGCAGATG TGTGCAAGCT TACCTGCAGA GCTAAGGGCA CAGGCTACTA TGTGGTCTTT 
TCTCCAAAGG TTACGGATGG GACTGAATGC AGGCCGTACA GCAACTCTGT GTGTGTCCGA 
GGACGGTGTG TGAGAACTGG ATGTGACGGC ATTATTGGCT CAAAGCTACA ATATGACAAG 
TGTGGAGTGT GCGGAGGGGA TAACTCCAGT TGTACAAAGA TTATCGGAAC CTTCAATAAA 
AAAAGCAAGG GTTATACTGA CGTTGTGAGG ATCCCTGAAG GAGCAACCCA CATAAAAGTC 
CGACAGTTCA AAGCCAAAGA CCAGACTAGA TTCACTGCCT ACTTAGCCCT GAAGAAGAAA 
ACTGGCGAGT ACCTTATCAA TGGCAAGTAC ATGATTTCCA CTTCAGAGAC CATCATCGAC 
ATCAATGGTA CCGTCATGAA CTACAGTGGA TGGAGCCACA GAGATGATTT TTTACATGGG 
ATGGGCTATT CAGCCACAAA AGAAATCCTG ATCGTGCAGA TCCTTGCCAC AGACCCAACT 
AAAGCGCTAG ACGTCCGTTA CAGCTTTTTT GTTCCCAAGA AGACCACTCA AAAAGTAAAC 
TCTGTCATCA GCCATGGCAG CAACAAGGTG GGACCACACT CTACACAGCT GCAGTGGGTG 
ACAGGTCCAT GGCTGGCCTG CTCCAGGACC TGTGACACAG GCTGGCACAC TAGGACCGTG 
CAGTGCCAGG ATGGAAACAG GAAATTAGCT AAAGGATGCC TTCTCTCTCA GAGGCCTTCT 
GCATTTAAGC AATGTCTGCT GAAGAAATGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035912.2
CDS	859..3651
Translation	MRLEWAPLLLLLLLLSASCLSLAADSPAAAPAQDKTRQPQAAAAAAEPDQPQGEETRERGHLQPLAGQRRSGGLVQNIDQLYSGGGKVGYLVYAGGRRFLLDLERDDTVGAAGSIVTAGGGLSASSGHRGHCFYRGTVDGSPRSLAVFDLCGGLDGFFAVKHARYTLKPLLRGSWAEYERIYGDGSSRILHVYNREGFSFEALPPRASCETPASPSGPQESPSVHSRSRRRSALAPQLLDHSAFSPSGNAGPQTWWRRRRRSISRARQVELLLVADSSMARMYGRGLQHYLLTLASIANRLYSHASIENHIRLAVVKVVVLTDKDTSLEVSKNAATTLKNFCKWQHQHNQLGDDHEEHYDAAILFTREDLCGHHSCDTLGMADVGTICSPERSCAVIEDDGLHAAFTVAHEIGHLLGLSHDDSKFCEENFGTTEDKRLMSSILTSIDASKPWSKCTSATITEFLDDGHGNCLLDLPRKQILGPEELPGQTYDATQQCNLTFGPEYSVCPGMDVCARLWCAVVRQGQMVCLTKKLPAVEGTPCGKGRVCLQGKCVDKTKKKYYSTSSHGNWGSWGPWGQCSRSCGGGVQFAYRHCNNPAPRNSGRYCTGKRAIYRSCSVTPCPPNGKSFRHEQCEAKNGYQSDAKGVKTFVEWVPKYAGVLPADVCKLTCRAKGTGYYVVFSPKVTDGTECRPYSNSVCVRGRCVRTGCDGIIGSKLQYDKCGVCGGDNSSCTKIIGTFNKKSKGYTDVVRIPEGATHIKVRQFKAKDQTRFTAYLALKKKTGEYLINGKYMISTSETIIDINGTVMNYSGWSHRDDFLHGMGYSATKEILIVQILATDPTKALDVRYSFFVPKKTTQKVNSVISHGSNKVGPHSTQLQWVTGPWLACSRTCDTGWHTRTVQCQDGNRKLAKGCLLSQRPSAFKQCLLKKC

Target ORF information:

RefSeq Version	NM_011782.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2) (Adamts5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011782.2

ORF Insert Sequence:

ATGCGGCTCG AGTGGGCGCC CTTGTTGCTG CTACTGCTGC TGCTGAGCGC GTCCTGCCTG 
TCCCTGGCCG CTGACAGCCC CGCCGCGGCA CCTGCCCAGG ATAAAACCAG GCAGCCTCAG 
GCTGCAGCAG CGGCCGCCGA GCCGGACCAG CCGCAGGGGG AGGAAACACG GGAGCGAGGC 
CATTTACAAC CCTTGGCCGG GCAGCGCAGG AGCGGCGGGC TGGTCCAGAA TATAGACCAA 
CTCTACTCTG GCGGTGGCAA AGTGGGCTAC CTTGTCTACG CGGGCGGCCG GAGGTTCCTG 
CTGGACCTGG AGAGAGATGA CACAGTGGGT GCTGCTGGTA GCATCGTTAC TGCAGGAGGA 
GGGCTGAGCG CATCCTCTGG CCACCGGGGT CACTGTTTCT ACAGAGGCAC CGTGGACGGC 
AGCCCTCGAT CCCTAGCTGT CTTTGACCTC TGCGGGGGTC TCGATGGCTT CTTTGCAGTC 
AAGCATGCGC GCTACACTCT AAAGCCACTC CTGCGTGGGT CCTGGGCAGA GTATGAACGA 
ATTTATGGGG ATGGATCTTC CCGCATCCTG CATGTCTACA ACCGCGAGGG CTTTAGCTTC 
GAGGCCCTGC CGCCACGCGC CAGTTGCGAG ACTCCTGCAT CCCCATCTGG GCCCCAAGAG 
AGCCCCTCGG TGCACAGTAG ATCTAGGAGA CGCTCAGCGC TGGCCCCGCA GCTGCTGGAC 
CACTCAGCTT TCTCGCCATC TGGGAACGCG GGACCTCAGA CTTGGTGGAG GCGTAGGCGC 
CGTTCCATCT CCAGGGCCCG CCAGGTGGAG CTCCTCTTGG TGGCTGACTC GTCCATGGCC 
AGGATGTATG GGCGGGGCCT GCAGCATTAC CTGCTGACCC TGGCCTCCAT CGCCAACAGG 
CTGTACAGTC ATGCAAGCAT TGAGAACCAC ATCCGCCTGG CGGTGGTGAA GGTGGTGGTG 
CTGACGGACA AGGACACGAG TCTGGAGGTG AGCAAGAATG CGGCCACGAC CCTCAAGAAC 
TTTTGCAAAT GGCAGCACCA ACATAACCAG CTAGGGGATG ATCACGAAGA GCACTACGAT 
GCAGCCATCC TGTTCACCCG AGAGGATTTA TGTGGGCATC ATTCATGTGA CACCCTGGGA 
ATGGCAGACG TTGGGACCAT ATGTTCTCCG GAGCGCAGCT GTGCAGTGAT TGAAGATGAT 
GGCCTCCATG CAGCCTTCAC TGTGGCTCAT GAAATTGGGC ATCTACTTGG CCTTTCTCAT 
GACGATTCCA AATTCTGTGA AGAGAACTTC GGTACTACAG AAGACAAGCG TTTAATGTCT 
TCAATCCTTA CCAGCATCGA TGCATCCAAG CCCTGGTCCA AATGCACGTC AGCCACCATC 
ACAGAATTCC TGGATGATGG TCATGGTAAT TGTTTGCTAG ACCTACCACG GAAGCAGATT 
TTGGGTCCCG AGGAACTCCC AGGACAGACC TACGATGCCA CCCAGCAGTG CAACTTGACA 
TTTGGGCCTG AGTACTCGGT GTGCCCTGGC ATGGATGTCT GTGCGCGGCT GTGGTGTGCT 
GTGGTGCGCC AAGGCCAAAT GGTGTGTCTG ACCAAGAAGC TGCCGGCTGT GGAGGGCACT 
CCCTGTGGGA AGGGAAGAGT CTGCCTTCAA GGCAAATGTG TGGACAAAAC TAAGAAAAAA 
TATTACTCGA CATCAAGCCA TGGAAATTGG GGGTCCTGGG GCCCCTGGGG TCAGTGTTCT 
CGCTCATGCG GGGGAGGAGT GCAGTTTGCC TACCGCCATT GTAATAACCC TGCACCTCGA 
AACAGTGGCC GCTACTGCAC AGGGAAGAGG GCCATATACC GTTCCTGCAG TGTTACACCC 
TGCCCACCCA ATGGTAAATC TTTTCGCCAT GAGCAGTGTG AAGCCAAAAA TGGCTATCAG 
TCTGATGCAA AAGGAGTCAA AACATTTGTA GAATGGGTTC CCAAATATGC AGGTGTCCTG 
CCGGCAGATG TGTGCAAGCT TACCTGCAGA GCTAAGGGCA CAGGCTACTA TGTGGTCTTT 
TCTCCAAAGG TTACGGATGG GACTGAATGC AGGCCGTACA GCAACTCTGT GTGTGTCCGA 
GGACGGTGTG TGAGAACTGG ATGTGACGGC ATTATTGGCT CAAAGCTACA ATATGACAAG 
TGTGGAGTGT GCGGAGGGGA TAACTCCAGT TGTACAAAGA TTATCGGAAC CTTCAATAAA 
AAAAGCAAGG GTTATACTGA CGTTGTGAGG ATCCCTGAAG GAGCAACCCA CATAAAAGTC 
CGACAGTTCA AAGCCAAAGA CCAGACTAGA TTCACTGCCT ACTTAGCCCT GAAGAAGAAA 
ACTGGCGAGT ACCTTATCAA TGGCAAGTAC ATGATTTCCA CTTCAGAGAC CATCATCGAC 
ATCAATGGTA CCGTCATGAA CTACAGTGGA TGGAGCCACA GAGATGATTT TTTACATGGG 
ATGGGCTATT CAGCCACAAA AGAAATCCTG ATCGTGCAGA TCCTTGCCAC AGACCCAACT 
AAAGCGCTAG ACGTCCGTTA CAGCTTTTTT GTTCCCAAGA AGACCACTCA AAAAGTAAAC 
TCTGTCATCA GCCATGGCAG CAACAAGGTG GGACCACACT CTACACAGCT GCAGTGGGTG 
ACAGGTCCAT GGCTGGCCTG CTCCAGGACC TGTGACACAG GCTGGCACAC TAGGACCGTG 
CAGTGCCAGG ATGGAAACAG GAAATTAGCT AAAGGATGCC TTCTCTCTCA GAGGCCTTCT 
GCATTTAAGC AATGTCTGCT GAAGAAATGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADAMTS5 ( XM_011246127.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_011244429.1 Mus musculus a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2) (Adamts5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu08840
Clone ID Related Accession (Same CDS sequence)	NM_011782.2 , XM_011246127.1
Accession Version	XM_011246127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 5 isoform X1
Comment

ATGCGGCTCG AGTGGGCGCC CTTGTTGCTG CTACTGCTGC TGCTGAGCGC GTCCTGCCTG 
TCCCTGGCCG CTGACAGCCC CGCCGCGGCA CCTGCCCAGG ATAAAACCAG GCAGCCTCAG 
GCTGCAGCAG CGGCCGCCGA GCCGGACCAG CCGCAGGGGG AGGAAACACG GGAGCGAGGC 
CATTTACAAC CCTTGGCCGG GCAGCGCAGG AGCGGCGGGC TGGTCCAGAA TATAGACCAA 
CTCTACTCTG GCGGTGGCAA AGTGGGCTAC CTTGTCTACG CGGGCGGCCG GAGGTTCCTG 
CTGGACCTGG AGAGAGATGA CACAGTGGGT GCTGCTGGTA GCATCGTTAC TGCAGGAGGA 
GGGCTGAGCG CATCCTCTGG CCACCGGGGT CACTGTTTCT ACAGAGGCAC CGTGGACGGC 
AGCCCTCGAT CCCTAGCTGT CTTTGACCTC TGCGGGGGTC TCGATGGCTT CTTTGCAGTC 
AAGCATGCGC GCTACACTCT AAAGCCACTC CTGCGTGGGT CCTGGGCAGA GTATGAACGA 
ATTTATGGGG ATGGATCTTC CCGCATCCTG CATGTCTACA ACCGCGAGGG CTTTAGCTTC 
GAGGCCCTGC CGCCACGCGC CAGTTGCGAG ACTCCTGCAT CCCCATCTGG GCCCCAAGAG 
AGCCCCTCGG TGCACAGTAG ATCTAGGAGA CGCTCAGCGC TGGCCCCGCA GCTGCTGGAC 
CACTCAGCTT TCTCGCCATC TGGGAACGCG GGACCTCAGA CTTGGTGGAG GCGTAGGCGC 
CGTTCCATCT CCAGGGCCCG CCAGGTGGAG CTCCTCTTGG TGGCTGACTC GTCCATGGCC 
AGGATGTATG GGCGGGGCCT GCAGCATTAC CTGCTGACCC TGGCCTCCAT CGCCAACAGG 
CTGTACAGTC ATGCAAGCAT TGAGAACCAC ATCCGCCTGG CGGTGGTGAA GGTGGTGGTG 
CTGACGGACA AGGACACGAG TCTGGAGGTG AGCAAGAATG CGGCCACGAC CCTCAAGAAC 
TTTTGCAAAT GGCAGCACCA ACATAACCAG CTAGGGGATG ATCACGAAGA GCACTACGAT 
GCAGCCATCC TGTTCACCCG AGAGGATTTA TGTGGGCATC ATTCATGTGA CACCCTGGGA 
ATGGCAGACG TTGGGACCAT ATGTTCTCCG GAGCGCAGCT GTGCAGTGAT TGAAGATGAT 
GGCCTCCATG CAGCCTTCAC TGTGGCTCAT GAAATTGGGC ATCTACTTGG CCTTTCTCAT 
GACGATTCCA AATTCTGTGA AGAGAACTTC GGTACTACAG AAGACAAGCG TTTAATGTCT 
TCAATCCTTA CCAGCATCGA TGCATCCAAG CCCTGGTCCA AATGCACGTC AGCCACCATC 
ACAGAATTCC TGGATGATGG TCATGGTAAT TGTTTGCTAG ACCTACCACG GAAGCAGATT 
TTGGGTCCCG AGGAACTCCC AGGACAGACC TACGATGCCA CCCAGCAGTG CAACTTGACA 
TTTGGGCCTG AGTACTCGGT GTGCCCTGGC ATGGATGTCT GTGCGCGGCT GTGGTGTGCT 
GTGGTGCGCC AAGGCCAAAT GGTGTGTCTG ACCAAGAAGC TGCCGGCTGT GGAGGGCACT 
CCCTGTGGGA AGGGAAGAGT CTGCCTTCAA GGCAAATGTG TGGACAAAAC TAAGAAAAAA 
TATTACTCGA CATCAAGCCA TGGAAATTGG GGGTCCTGGG GCCCCTGGGG TCAGTGTTCT 
CGCTCATGCG GGGGAGGAGT GCAGTTTGCC TACCGCCATT GTAATAACCC TGCACCTCGA 
AACAGTGGCC GCTACTGCAC AGGGAAGAGG GCCATATACC GTTCCTGCAG TGTTACACCC 
TGCCCACCCA ATGGTAAATC TTTTCGCCAT GAGCAGTGTG AAGCCAAAAA TGGCTATCAG 
TCTGATGCAA AAGGAGTCAA AACATTTGTA GAATGGGTTC CCAAATATGC AGGTGTCCTG 
CCGGCAGATG TGTGCAAGCT TACCTGCAGA GCTAAGGGCA CAGGCTACTA TGTGGTCTTT 
TCTCCAAAGG TTACGGATGG GACTGAATGC AGGCCGTACA GCAACTCTGT GTGTGTCCGA 
GGACGGTGTG TGAGAACTGG ATGTGACGGC ATTATTGGCT CAAAGCTACA ATATGACAAG 
TGTGGAGTGT GCGGAGGGGA TAACTCCAGT TGTACAAAGA TTATCGGAAC CTTCAATAAA 
AAAAGCAAGG GTTATACTGA CGTTGTGAGG ATCCCTGAAG GAGCAACCCA CATAAAAGTC 
CGACAGTTCA AAGCCAAAGA CCAGACTAGA TTCACTGCCT ACTTAGCCCT GAAGAAGAAA 
ACTGGCGAGT ACCTTATCAA TGGCAAGTAC ATGATTTCCA CTTCAGAGAC CATCATCGAC 
ATCAATGGTA CCGTCATGAA CTACAGTGGA TGGAGCCACA GAGATGATTT TTTACATGGG 
ATGGGCTATT CAGCCACAAA AGAAATCCTG ATCGTGCAGA TCCTTGCCAC AGACCCAACT 
AAAGCGCTAG ACGTCCGTTA CAGCTTTTTT GTTCCCAAGA AGACCACTCA AAAAGTAAAC 
TCTGTCATCA GCCATGGCAG CAACAAGGTG GGACCACACT CTACACAGCT GCAGTGGGTG 
ACAGGTCCAT GGCTGGCCTG CTCCAGGACC TGTGACACAG GCTGGCACAC TAGGACCGTG 
CAGTGCCAGG ATGGAAACAG GAAATTAGCT AAAGGATGCC TTCTCTCTCA GAGGCCTTCT 
GCATTTAAGC AATGTCTGCT GAAGAAATGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011244429.1

Translation

MRLEWAPLLLLLLLLSASCLSLAADSPAAAPAQDKTRQPQAAAAAAEPDQPQGEETRERGHLQPLAGQRRSGGLVQNIDQLYSGGGKVGYLVYAGGRRFLLDLERDDTVGAAGSIVTAGGGLSASSGHRGHCFYRGTVDGSPRSLAVFDLCGGLDGFFAVKHARYTLKPLLRGSWAEYERIYGDGSSRILHVYNREGFSFEALPPRASCETPASPSGPQESPSVHSRSRRRSALAPQLLDHSAFSPSGNAGPQTWWRRRRRSISRARQVELLLVADSSMARMYGRGLQHYLLTLASIANRLYSHASIENHIRLAVVKVVVLTDKDTSLEVSKNAATTLKNFCKWQHQHNQLGDDHEEHYDAAILFTREDLCGHHSCDTLGMADVGTICSPERSCAVIEDDGLHAAFTVAHEIGHLLGLSHDDSKFCEENFGTTEDKRLMSSILTSIDASKPWSKCTSATITEFLDDGHGNCLLDLPRKQILGPEELPGQTYDATQQCNLTFGPEYSVCPGMDVCARLWCAVVRQGQMVCLTKKLPAVEGTPCGKGRVCLQGKCVDKTKKKYYSTSSHGNWGSWGPWGQCSRSCGGGVQFAYRHCNNPAPRNSGRYCTGKRAIYRSCSVTPCPPNGKSFRHEQCEAKNGYQSDAKGVKTFVEWVPKYAGVLPADVCKLTCRAKGTGYYVVFSPKVTDGTECRPYSNSVCVRGRCVRTGCDGIIGSKLQYDKCGVCGGDNSSCTKIIGTFNKKSKGYTDVVRIPEGATHIKVRQFKAKDQTRFTAYLALKKKTGEYLINGKYMISTSETIIDINGTVMNYSGWSHRDDFLHGMGYSATKEILIVQILATDPTKALDVRYSFFVPKKTTQKVNSVISHGSNKVGPHSTQLQWVTGPWLACSRTCDTGWHTRTVQCQDGNRKLAKGCLLSQRPSAFKQCLLKKC

Target ORF information:

RefSeq Version	XM_011246127.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2) (Adamts5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246127.1

ORF Insert Sequence:

ATGCGGCTCG AGTGGGCGCC CTTGTTGCTG CTACTGCTGC TGCTGAGCGC GTCCTGCCTG 
TCCCTGGCCG CTGACAGCCC CGCCGCGGCA CCTGCCCAGG ATAAAACCAG GCAGCCTCAG 
GCTGCAGCAG CGGCCGCCGA GCCGGACCAG CCGCAGGGGG AGGAAACACG GGAGCGAGGC 
CATTTACAAC CCTTGGCCGG GCAGCGCAGG AGCGGCGGGC TGGTCCAGAA TATAGACCAA 
CTCTACTCTG GCGGTGGCAA AGTGGGCTAC CTTGTCTACG CGGGCGGCCG GAGGTTCCTG 
CTGGACCTGG AGAGAGATGA CACAGTGGGT GCTGCTGGTA GCATCGTTAC TGCAGGAGGA 
GGGCTGAGCG CATCCTCTGG CCACCGGGGT CACTGTTTCT ACAGAGGCAC CGTGGACGGC 
AGCCCTCGAT CCCTAGCTGT CTTTGACCTC TGCGGGGGTC TCGATGGCTT CTTTGCAGTC 
AAGCATGCGC GCTACACTCT AAAGCCACTC CTGCGTGGGT CCTGGGCAGA GTATGAACGA 
ATTTATGGGG ATGGATCTTC CCGCATCCTG CATGTCTACA ACCGCGAGGG CTTTAGCTTC 
GAGGCCCTGC CGCCACGCGC CAGTTGCGAG ACTCCTGCAT CCCCATCTGG GCCCCAAGAG 
AGCCCCTCGG TGCACAGTAG ATCTAGGAGA CGCTCAGCGC TGGCCCCGCA GCTGCTGGAC 
CACTCAGCTT TCTCGCCATC TGGGAACGCG GGACCTCAGA CTTGGTGGAG GCGTAGGCGC 
CGTTCCATCT CCAGGGCCCG CCAGGTGGAG CTCCTCTTGG TGGCTGACTC GTCCATGGCC 
AGGATGTATG GGCGGGGCCT GCAGCATTAC CTGCTGACCC TGGCCTCCAT CGCCAACAGG 
CTGTACAGTC ATGCAAGCAT TGAGAACCAC ATCCGCCTGG CGGTGGTGAA GGTGGTGGTG 
CTGACGGACA AGGACACGAG TCTGGAGGTG AGCAAGAATG CGGCCACGAC CCTCAAGAAC 
TTTTGCAAAT GGCAGCACCA ACATAACCAG CTAGGGGATG ATCACGAAGA GCACTACGAT 
GCAGCCATCC TGTTCACCCG AGAGGATTTA TGTGGGCATC ATTCATGTGA CACCCTGGGA 
ATGGCAGACG TTGGGACCAT ATGTTCTCCG GAGCGCAGCT GTGCAGTGAT TGAAGATGAT 
GGCCTCCATG CAGCCTTCAC TGTGGCTCAT GAAATTGGGC ATCTACTTGG CCTTTCTCAT 
GACGATTCCA AATTCTGTGA AGAGAACTTC GGTACTACAG AAGACAAGCG TTTAATGTCT 
TCAATCCTTA CCAGCATCGA TGCATCCAAG CCCTGGTCCA AATGCACGTC AGCCACCATC 
ACAGAATTCC TGGATGATGG TCATGGTAAT TGTTTGCTAG ACCTACCACG GAAGCAGATT 
TTGGGTCCCG AGGAACTCCC AGGACAGACC TACGATGCCA CCCAGCAGTG CAACTTGACA 
TTTGGGCCTG AGTACTCGGT GTGCCCTGGC ATGGATGTCT GTGCGCGGCT GTGGTGTGCT 
GTGGTGCGCC AAGGCCAAAT GGTGTGTCTG ACCAAGAAGC TGCCGGCTGT GGAGGGCACT 
CCCTGTGGGA AGGGAAGAGT CTGCCTTCAA GGCAAATGTG TGGACAAAAC TAAGAAAAAA 
TATTACTCGA CATCAAGCCA TGGAAATTGG GGGTCCTGGG GCCCCTGGGG TCAGTGTTCT 
CGCTCATGCG GGGGAGGAGT GCAGTTTGCC TACCGCCATT GTAATAACCC TGCACCTCGA 
AACAGTGGCC GCTACTGCAC AGGGAAGAGG GCCATATACC GTTCCTGCAG TGTTACACCC 
TGCCCACCCA ATGGTAAATC TTTTCGCCAT GAGCAGTGTG AAGCCAAAAA TGGCTATCAG 
TCTGATGCAA AAGGAGTCAA AACATTTGTA GAATGGGTTC CCAAATATGC AGGTGTCCTG 
CCGGCAGATG TGTGCAAGCT TACCTGCAGA GCTAAGGGCA CAGGCTACTA TGTGGTCTTT 
TCTCCAAAGG TTACGGATGG GACTGAATGC AGGCCGTACA GCAACTCTGT GTGTGTCCGA 
GGACGGTGTG TGAGAACTGG ATGTGACGGC ATTATTGGCT CAAAGCTACA ATATGACAAG 
TGTGGAGTGT GCGGAGGGGA TAACTCCAGT TGTACAAAGA TTATCGGAAC CTTCAATAAA 
AAAAGCAAGG GTTATACTGA CGTTGTGAGG ATCCCTGAAG GAGCAACCCA CATAAAAGTC 
CGACAGTTCA AAGCCAAAGA CCAGACTAGA TTCACTGCCT ACTTAGCCCT GAAGAAGAAA 
ACTGGCGAGT ACCTTATCAA TGGCAAGTAC ATGATTTCCA CTTCAGAGAC CATCATCGAC 
ATCAATGGTA CCGTCATGAA CTACAGTGGA TGGAGCCACA GAGATGATTT TTTACATGGG 
ATGGGCTATT CAGCCACAAA AGAAATCCTG ATCGTGCAGA TCCTTGCCAC AGACCCAACT 
AAAGCGCTAG ACGTCCGTTA CAGCTTTTTT GTTCCCAAGA AGACCACTCA AAAAGTAAAC 
TCTGTCATCA GCCATGGCAG CAACAAGGTG GGACCACACT CTACACAGCT GCAGTGGGTG 
ACAGGTCCAT GGCTGGCCTG CTCCAGGACC TGTGACACAG GCTGGCACAC TAGGACCGTG 
CAGTGCCAGG ATGGAAACAG GAAATTAGCT AAAGGATGCC TTCTCTCTCA GAGGCCTTCT 
GCATTTAAGC AATGTCTGCT GAAGAAATGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ADAMTS5 cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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