Lrfn3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lrfn3
Entrez Gene ID	233067
Full Name	leucine rich repeat and fibronectin type III domain containing 3
Synonyms	A530045B06Rik,SALM4
General protein information	Preferred Names leucine rich repeat and fibronectin type III domain containing 3 Names leucine-rich repeat and fibronectin type-III domain-containing protein 3 synaptic adhesion-like molecule 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_175478.2	NP_780687.1	leucine-rich repeat and fibronectin type-III domain-containing protein 3 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	LRFN3	NP_078785.1
Pan troglodytes (chimpanzee)	LRFN3	XP_524229.1
Mus musculus (house mouse)	Lrfn3	NP_780687.1
Danio rerio (zebrafish)	zgc:172282	NP_001107121.1
Canis lupus familiaris (dog)	LRFN3	XP_005616950.1
Bos taurus (cattle)	LRFN3	NP_001070427.1
Danio rerio (zebrafish)	LOC101882083	XP_005157887.1
Xenopus tropicalis (tropical clawed frog)	LOC100486910	XP_002940406.1
	LRFN3	XP_001100482.1
Rattus norvegicus (Norway rat)	Lrfn3	NP_001100972.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

SALM4 suppresses excitatory synapse development by cis-inhibiting trans-synaptic SALM3-LAR adhesion.
Lie E, Ko JS, Choi SY, Roh JD, Cho YS, Noh R, Kim D, Li Y, Kang H, Choi TY, Nam J, Mah W, Lee D, Lee SG, Kim HM, Kim H, Choi SY, Um JW, Kang MG, Bae YC, Ko J, Kim E
Nature communications712328(2016 Aug)

Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion.
Gu Z, Imai F, Kim IJ, Fujita H, Katayama Ki, Mori K, Yoshihara Y, Yoshida Y
PloS one10(3)e0121550(2015)

Selected SALM (synaptic adhesion-like molecule) family proteins regulate synapse formation.
Mah W, Ko J, Nam J, Han K, Chung WS, Kim E
The Journal of neuroscience : the official journal of the Society for Neuroscience30(16)5559-68(2010 Apr)

Expression pattern of LRR and Ig domain-containing protein (LRRIG protein) in the early mouse embryo.
Homma S, Shimada T, Hikake T, Yaginuma H
Gene expression patterns : GEP9(1)1-26(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that the C-terminal PDZ binding domains of synaptic adhesion-like molecules (SALMs)1-3 are required for neurite outgrowth, and that the N-termini of SALMs 2 and 4 are alsoinvolved in neurite outgrowth.
Title: Synaptic adhesion-like molecules (SALMs) promote neurite outgrowth.

The following Lrfn3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lrfn3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu05455	NM_175478.2 Latest version!	Mus musculus leucine rich repeat and fibronectin type III domain containing 3 (Lrfn3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$370.30 $529.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lrfn3 ( NM_175478.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780687.1 Mus musculus leucine rich repeat and fibronectin type III domain containing 3 (Lrfn3), mRNA.

Price & Availability↑

CloneID	OMu05455
Clone ID Related Accession (Same CDS sequence)	NM_175478.2
Accession Version	NM_175478.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	leucine-rich repeat and fibronectin type-III domain-containing protein 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC149067.3. On Nov 17, 2006 this sequence version replaced NM_175478.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK040916.1, BC094593.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGTCC TTCCACTACT CCTTTGCCTG CTACCGCTGG CCCCCGCCTC ATCTCCACCC 
CAGCCGGCCA TATCCAGCCC CTGTCCCCGC CGTTGCCGCT GCCAGACCCA GTCGATGCCC 
CTAAGCGTGT TGTGCCCAGG GGCGGGCCTT CTATTCGTAC CGCCATCACT GGATCGCCGC 
GCTGCAGAAC TGCGCCTGGC AGACAACTTC ATCGCGGCAG TGCGTCGTCG AGACCTGGCC 
AACATGACAG GCCTGCTGCA TCTGAGCTTG TCTAGAAACA CCATCCGCCA TGTGGCAGCT 
GGCGCCTTCG CCGACCTACG TGCCCTGCGT GCCCTGCACC TAGATGGTAA TAGACTGACC 
TCGCTGGGCG AGGGTCAGTT GCGCGGCTTA GTCAACTTGC GCCACCTCAT CCTGAGCAAC 
AACCAGCTAG CAGCCCTGGC AGCTGGTGCC CTAGACGACT GTGCCGAGAC CCTGGAGGAC 
CTCGATCTCT CTTATAATAA CCTTGAGCAG CTTCCCTGGG AGGCTTTAGG GCGCCTAGGC 
AACGTCAACA CGTTGGGCCT TGACCACAAT TTGTTGGCTT CAGTGCCCGC TGGAGCCTTT 
TCCCGCCTGC ACAAGTTGGC ACGACTGGAC ATGACCTCCA ACCGCCTAAC CACCATCCCT 
CCTGACCCAC TCTTCTCACG TTTGCCACTG CTTGCCCGGC CTCGAGGCTC GCCAGCCTCT 
GCTCTGGTGC TGGCCTTTGG GGGAAACCCC CTTCACTGCA ACTGTGAGCT GGTGTGGCTG 
CGACGCCTGG CTCGGGAGGA CGACCTCGAG GCCTGTGCCT CGCCACCTGC TCTGGGGGGC 
CGCTACTTCT GGGCTGTGGG TGAGGAGGAG TTTGTGTGTG AGCCACCTGT GGTGACCCAC 
CGCTCACCTC CCCTGGCAGT GCCCGCAGGT CGGCCAGCTG CCCTGCGATG CCGGGCAGTA 
GGGGACCCAG AGCCACGAGT GCGTTGGGTA TCACCCCAGG GTCGGCTGCT GGGCAACTCC 
AGCCGTGCTC GTGCCTTCCC TAATGGGACC CTGGAGCTAC TGGTCACTGA GCCGGAAGAT 
GGCGGCACTT TCACTTGCAT TGCAGCCAAT GCCGCTGGCG AGGCCACTGC TGCTGTTGAG 
CTGACCGTGG GTCCCCCTCC ACCCCCCCAG CTAGCCAATA GCACCAGCTG TGACCCCCCG 
CGGGACGGGG AACCCGATGC CCTCACCCCG CCCTCTGCGG CTTCAGCCTC CGCCAAAGTG 
GCGGACACTG TGGCCCCTAC CGACCGTGGC GTCCAAGTGA CTGAGCATGG GGCCACAGCG 
GCTCTTGTTC AGTGGCCAGA TCAACGGCCT GTCCCAGGCA TCCGCATGTA CCAGATCCAG 
TACAACAGCT CGGCCGACGA CATCCTTGTC TACAGGATGA TCCCAGCGGA CAGTCGCTCC 
TTCCTGCTGA CCGATCTGGC GTCAGGCCGT ACCTATGATC TATGTGTGCT CGCAGTCTAT 
GAGGACAGCG CCACCGGGCT AACGGCCACG CGGCCAGTGG GCTGCGCTCG GTTCTCCACG 
GAGCCCGCAC TTCGGCCTTG TGCTGCACCG CACGCACCCT TCCTGGGCGG CACCATGATC 
ATAGCGCTGG GAGGTGTCAT CGTCGCTTCT GTTCTGGTCT TCATCTTCGT GCTGCTTCTA 
CGTTACAAGG TGCACGGAGG CCAGCCGCCC GGCAAGGCCA AGGCTACGGC ACCTGTCAGC 
AGCGTTTGCT CCCAGACCAA CGGAGCGCTC GGGCCCGTGC CCAGTGCGCC TGCCCCTGAG 
CCTGCTGCAC CCAGGGCCCA TACTGTGGTC CAATTGGACT GTGAGCCCTG GGGGCCCAGT 
CACGAACCTG CAGGACCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780687.1
CDS	857..2737
Translation	MAVLPLLLCLLPLAPASSPPQPAISSPCPRRCRCQTQSMPLSVLCPGAGLLFVPPSLDRRAAELRLADNFIAAVRRRDLANMTGLLHLSLSRNTIRHVAAGAFADLRALRALHLDGNRLTSLGEGQLRGLVNLRHLILSNNQLAALAAGALDDCAETLEDLDLSYNNLEQLPWEALGRLGNVNTLGLDHNLLASVPAGAFSRLHKLARLDMTSNRLTTIPPDPLFSRLPLLARPRGSPASALVLAFGGNPLHCNCELVWLRRLAREDDLEACASPPALGGRYFWAVGEEEFVCEPPVVTHRSPPLAVPAGRPAALRCRAVGDPEPRVRWVSPQGRLLGNSSRARAFPNGTLELLVTEPEDGGTFTCIAANAAGEATAAVELTVGPPPPPQLANSTSCDPPRDGEPDALTPPSAASASAKVADTVAPTDRGVQVTEHGATAALVQWPDQRPVPGIRMYQIQYNSSADDILVYRMIPADSRSFLLTDLASGRTYDLCVLAVYEDSATGLTATRPVGCARFSTEPALRPCAAPHAPFLGGTMIIALGGVIVASVLVFIFVLLLRYKVHGGQPPGKAKATAPVSSVCSQTNGALGPVPSAPAPEPAAPRAHTVVQLDCEPWGPSHEPAGP

Target ORF information:

RefSeq Version	NM_175478.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine rich repeat and fibronectin type III domain containing 3 (Lrfn3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175478.2

ORF Insert Sequence:

ATGGCCGTCC TTCCACTACT CCTTTGCCTG CTACCGCTGG CCCCCGCCTC ATCTCCACCC 
CAGCCGGCCA TATCCAGCCC CTGTCCCCGC CGTTGCCGCT GCCAGACCCA GTCGATGCCC 
CTAAGCGTGT TGTGCCCAGG GGCGGGCCTT CTATTCGTAC CGCCATCACT GGATCGCCGC 
GCTGCAGAAC TGCGCCTGGC AGACAACTTC ATCGCGGCAG TGCGTCGTCG AGACCTGGCC 
AACATGACAG GCCTGCTGCA TCTGAGCTTG TCTAGAAACA CCATCCGCCA TGTGGCAGCT 
GGCGCCTTCG CCGACCTACG TGCCCTGCGT GCCCTGCACC TAGATGGTAA TAGACTGACC 
TCGCTGGGCG AGGGTCAGTT GCGCGGCTTA GTCAACTTGC GCCACCTCAT CCTGAGCAAC 
AACCAGCTAG CAGCCCTGGC AGCTGGTGCC CTAGACGACT GTGCCGAGAC CCTGGAGGAC 
CTCGATCTCT CTTATAATAA CCTTGAGCAG CTTCCCTGGG AGGCTTTAGG GCGCCTAGGC 
AACGTCAACA CGTTGGGCCT TGACCACAAT TTGTTGGCTT CAGTGCCCGC TGGAGCCTTT 
TCCCGCCTGC ACAAGTTGGC ACGACTGGAC ATGACCTCCA ACCGCCTAAC CACCATCCCT 
CCTGACCCAC TCTTCTCACG TTTGCCACTG CTTGCCCGGC CTCGAGGCTC GCCAGCCTCT 
GCTCTGGTGC TGGCCTTTGG GGGAAACCCC CTTCACTGCA ACTGTGAGCT GGTGTGGCTG 
CGACGCCTGG CTCGGGAGGA CGACCTCGAG GCCTGTGCCT CGCCACCTGC TCTGGGGGGC 
CGCTACTTCT GGGCTGTGGG TGAGGAGGAG TTTGTGTGTG AGCCACCTGT GGTGACCCAC 
CGCTCACCTC CCCTGGCAGT GCCCGCAGGT CGGCCAGCTG CCCTGCGATG CCGGGCAGTA 
GGGGACCCAG AGCCACGAGT GCGTTGGGTA TCACCCCAGG GTCGGCTGCT GGGCAACTCC 
AGCCGTGCTC GTGCCTTCCC TAATGGGACC CTGGAGCTAC TGGTCACTGA GCCGGAAGAT 
GGCGGCACTT TCACTTGCAT TGCAGCCAAT GCCGCTGGCG AGGCCACTGC TGCTGTTGAG 
CTGACCGTGG GTCCCCCTCC ACCCCCCCAG CTAGCCAATA GCACCAGCTG TGACCCCCCG 
CGGGACGGGG AACCCGATGC CCTCACCCCG CCCTCTGCGG CTTCAGCCTC CGCCAAAGTG 
GCGGACACTG TGGCCCCTAC CGACCGTGGC GTCCAAGTGA CTGAGCATGG GGCCACAGCG 
GCTCTTGTTC AGTGGCCAGA TCAACGGCCT GTCCCAGGCA TCCGCATGTA CCAGATCCAG 
TACAACAGCT CGGCCGACGA CATCCTTGTC TACAGGATGA TCCCAGCGGA CAGTCGCTCC 
TTCCTGCTGA CCGATCTGGC GTCAGGCCGT ACCTATGATC TATGTGTGCT CGCAGTCTAT 
GAGGACAGCG CCACCGGGCT AACGGCCACG CGGCCAGTGG GCTGCGCTCG GTTCTCCACG 
GAGCCCGCAC TTCGGCCTTG TGCTGCACCG CACGCACCCT TCCTGGGCGG CACCATGATC 
ATAGCGCTGG GAGGTGTCAT CGTCGCTTCT GTTCTGGTCT TCATCTTCGT GCTGCTTCTA 
CGTTACAAGG TGCACGGAGG CCAGCCGCCC GGCAAGGCCA AGGCTACGGC ACCTGTCAGC 
AGCGTTTGCT CCCAGACCAA CGGAGCGCTC GGGCCCGTGC CCAGTGCGCC TGCCCCTGAG 
CCTGCTGCAC CCAGGGCCCA TACTGTGGTC CAATTGGACT GTGAGCCCTG GGGGCCCAGT 
CACGAACCTG CAGGACCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

SALM4 suppresses excitatory synapse development by cis-inhibiting trans-synaptic SALM3-LAR adhesion.
Nature communications712328(2016 Aug)
Lie E,Ko JS,Choi SY,Roh JD,Cho YS,Noh R,Kim D,Li Y,Kang H,Choi TY,Nam J,Mah W,Lee D,Lee SG,Kim HM,Kim H,Choi SY,Um JW,Kang MG,Bae YC,Ko J,Kim E

Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion.
PloS one10(3)e0121550(2015)
Gu Z,Imai F,Kim IJ,Fujita H,Katayama Ki,Mori K,Yoshihara Y,Yoshida Y

Selected SALM (synaptic adhesion-like molecule) family proteins regulate synapse formation.
The Journal of neuroscience : the official journal of the Society for Neuroscience30(16)5559-68(2010 Apr)
Mah W,Ko J,Nam J,Han K,Chung WS,Kim E

Expression pattern of LRR and Ig domain-containing protein (LRRIG protein) in the early mouse embryo.
Gene expression patterns : GEP9(1)1-26(2009 Jan)
Homma S,Shimada T,Hikake T,Yaginuma H