Insig1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Insig1
Entrez Gene ID	231070
Full Name	insulin induced gene 1
Synonyms	1810013C12Rik,Insig-1
General protein information	Preferred Names insulin induced gene 1 Names insulin-induced gene 1 protein INSIG-1 membrane protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_153526.5	NP_705746.1	insulin-induced gene 1 protein

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	insig1	NP_956163.1
Canis lupus familiaris (dog)	INSIG1	XP_005629762.1
Homo sapiens (human)	INSIG1	NP_005533.2
Pan troglodytes (chimpanzee)	INSIG1	XP_001145726.1
Gallus gallus (chicken)	INSIG1	NP_001026137.1
Xenopus tropicalis (tropical clawed frog)	insig1	NP_001072938.1
Bos taurus (cattle)	INSIG1	NP_001071377.1
Mus musculus (house mouse)	Insig1	NP_705746.1
Rattus norvegicus (Norway rat)	Insig1	NP_071787.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of PERK-eIF2? signalling by tuberous sclerosis complex-1 controls homoeostasis and survival of myelinating oligodendrocytes.
Jiang M, Liu L, He X, Wang H, Lin W, Wang H, Yoon SO, Wood TL, Lu QR
Nature communications712185(2016 Jul)

Mfsd2a Is a Transporter for the Essential ?-3 Fatty Acid Docosahexaenoic Acid (DHA) in Eye and Is Important for Photoreceptor Cell Development.
Wong BH, Chan JP, Cazenave-Gassiot A, Poh RW, Foo JC, Galam DL, Ghosh S, Nguyen LN, Barathi VA, Yeo SW, Luu CD, Wenk MR, Silver DL
The Journal of biological chemistry291(20)10501-14(2016 May)

SREBP-2-deficient and hypomorphic mice reveal roles for SREBP-2 in embryonic development and SREBP-1c expression.
Vergnes L, Chin RG, de Aguiar Vallim T, Fong LG, Osborne TF, Young SG, Reue K
Journal of lipid research57(3)410-21(2016 Mar)

The E3 ubiquitin ligase AMFR and INSIG1 bridge the activation of TBK1 kinase by modifying the adaptor STING.
Wang Q, Liu X, Cui Y, Tang Y, Chen W, Li S, Yu H, Pan Y, Wang C
Immunity41(6)919-33(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

myeloid-cell-specific Insig1-/- mice are more susceptible to HSV-1 infection
Title: The E3 ubiquitin ligase AMFR and INSIG1 bridge the activation of TBK1 kinase by modifying the adaptor STING.

the essential role of Insig1,2 proteins in the sterol homeostasis of enterocytes.
Title: Insig proteins mediate feedback inhibition of cholesterol synthesis in the intestine.

reducing Insig1 mRNA to a similar degree observed in morbidly obese human WAT accelerates adipocyte differentiation and enhances lipogenesis, and consequently, lipid accumulation
Title: Adaptive changes of the Insig1/SREBP1/SCD1 set point help adipose tissue to cope with increased storage demands of obesity.

we consistently observe involvement of gp78 in Insig-1 degradation, we find no substantive evidence to support roles for either gp78 or TRC8 in the robust sterol-accelerated degradation of HMG-CoA reductase.
Title: Differential regulation of HMG-CoA reductase and Insig-1 by enzymes of the ubiquitin-proteasome system.

analysis of mRNA abundance and expression of SLC27A, ACC, SCD, FADS, LPIN, INSIG, and PPARGC1 gene isoforms in mouse mammary glands during the lactation cycle
Title: mRNA abundance and expression of SLC27A, ACC, SCD, FADS, LPIN, INSIG, and PPARGC1 gene isoforms in mouse mammary glands during the lactation cycle.

The following Insig1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Insig1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15956	NM_153526.5 Latest version!	Mus musculus insulin induced gene 1 (Insig1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Insig1 ( NM_153526.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_705746.1 Mus musculus insulin induced gene 1 (Insig1), mRNA.

Price & Availability↑

CloneID	OMu15956
Clone ID Related Accession (Same CDS sequence)	NM_153526.5
Accession Version	NM_153526.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	insulin-induced gene 1 protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC079559.1 and AK151186.1. On Oct 24, 2007 this sequence version replaced NM_153526.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC079559.1, AK151186.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCAGGC TGCACGACCA CGTCTGGAAC TATCCAAGCG CGGGCGCTGC GAGGCCGTAC 
AGCCTCCCGC GAGGCATGAT TGCGGCGGCC GCTTGTCCGC AGGGCCCCGG AGTCCCCGAA 
CCCGAGCACG CGCCCCGGGG CCAGCGAGCG GGAACCACAG GATGCAGCGC GCGGCCGGGC 
AGCTGGCACC ACGACTTGGT GCAGCGGAGC CTAGTGCTCT TCTCATTTGG CGTGGTCCTG 
GCCCTGGTGC TCAACCTGCT GCAGATCCAG CGGAATGTCA CGCTCTTCCC CGACGAGGTG 
ATAGCCACCA TCTTCTCCTC CGCCTGGTGG GTGCCCCCGT GCTGTGGCAC GGCAGCGGCT 
GTTGTCGGTT TACTGTATCC CTGTATTGAC AGTCACCTGG GAGAACCACA CAAGTTCAAG 
AGAGAATGGG CCAGCGTTAT GCGCTGTATT GCCGTGTTCG TTGGCATCAA CCACGCCAGT 
GCCAAATTAG ATTTTGCCAA TAATGTCCAG CTGTCCTTGA CTTTAGCAGC CCTCTCTTTG 
GGTTTGTGGT GGACATTTGA TCGTTCCCGA AGCGGCCTTG GGCTTGGGAT TACCATCGCC 
TTCCTAGCTA CGCTGATCAC TCAGTTTCTT GTGTATAATG GTGTCTACCA GTACACGTCC 
CCTGATTTCC TCTATATCCG TTCTTGGCTC CCCTGTATAT TTTTCTCAGG AGGTGTCACA 
GTGGGAAACA TAGGACGACA GTTAGCTATG GGTGTTCCGG AAAAGCCTCA CAGTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_705746.1
CDS	27..806
Translation	MPRLHDHVWNYPSAGAARPYSLPRGMIAAAACPQGPGVPEPEHAPRGQRAGTTGCSARPGSWHHDLVQRSLVLFSFGVVLALVLNLLQIQRNVTLFPDEVIATIFSSAWWVPPCCGTAAAVVGLLYPCIDSHLGEPHKFKREWASVMRCIAVFVGINHASAKLDFANNVQLSLTLAALSLGLWWTFDRSRSGLGLGITIAFLATLITQFLVYNGVYQYTSPDFLYIRSWLPCIFFSGGVTVGNIGRQLAMGVPEKPHSD

Target ORF information:

RefSeq Version	NM_153526.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus insulin induced gene 1 (Insig1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153526.5

ORF Insert Sequence:

ATGCCCAGGC TGCACGACCA CGTCTGGAAC TATCCAAGCG CGGGCGCTGC GAGGCCGTAC 
AGCCTCCCGC GAGGCATGAT TGCGGCGGCC GCTTGTCCGC AGGGCCCCGG AGTCCCCGAA 
CCCGAGCACG CGCCCCGGGG CCAGCGAGCG GGAACCACAG GATGCAGCGC GCGGCCGGGC 
AGCTGGCACC ACGACTTGGT GCAGCGGAGC CTAGTGCTCT TCTCATTTGG CGTGGTCCTG 
GCCCTGGTGC TCAACCTGCT GCAGATCCAG CGGAATGTCA CGCTCTTCCC CGACGAGGTG 
ATAGCCACCA TCTTCTCCTC CGCCTGGTGG GTGCCCCCGT GCTGTGGCAC GGCAGCGGCT 
GTTGTCGGTT TACTGTATCC CTGTATTGAC AGTCACCTGG GAGAACCACA CAAGTTCAAG 
AGAGAATGGG CCAGCGTTAT GCGCTGTATT GCCGTGTTCG TTGGCATCAA CCACGCCAGT 
GCCAAATTAG ATTTTGCCAA TAATGTCCAG CTGTCCTTGA CTTTAGCAGC CCTCTCTTTG 
GGTTTGTGGT GGACATTTGA TCGTTCCCGA AGCGGCCTTG GGCTTGGGAT TACCATCGCC 
TTCCTAGCTA CGCTGATCAC TCAGTTTCTT GTGTATAATG GTGTCTACCA GTACACGTCC 
CCTGATTTCC TCTATATCCG TTCTTGGCTC CCCTGTATAT TTTTCTCAGG AGGTGTCACA 
GTGGGAAACA TAGGACGACA GTTAGCTATG GGTGTTCCGG AAAAGCCTCA CAGTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of PERK-eIF2? signalling by tuberous sclerosis complex-1 controls homoeostasis and survival of myelinating oligodendrocytes.
Nature communications712185(2016 Jul)
Jiang M,Liu L,He X,Wang H,Lin W,Wang H,Yoon SO,Wood TL,Lu QR

Mfsd2a Is a Transporter for the Essential ?-3 Fatty Acid Docosahexaenoic Acid (DHA) in Eye and Is Important for Photoreceptor Cell Development.
The Journal of biological chemistry291(20)10501-14(2016 May)
Wong BH,Chan JP,Cazenave-Gassiot A,Poh RW,Foo JC,Galam DL,Ghosh S,Nguyen LN,Barathi VA,Yeo SW,Luu CD,Wenk MR,Silver DL

SREBP-2-deficient and hypomorphic mice reveal roles for SREBP-2 in embryonic development and SREBP-1c expression.
Journal of lipid research57(3)410-21(2016 Mar)
Vergnes L,Chin RG,de Aguiar Vallim T,Fong LG,Osborne TF,Young SG,Reue K

The E3 ubiquitin ligase AMFR and INSIG1 bridge the activation of TBK1 kinase by modifying the adaptor STING.
Immunity41(6)919-33(2014 Dec)
Wang Q,Liu X,Cui Y,Tang Y,Chen W,Li S,Yu H,Pan Y,Wang C

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Insig1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報