Spout1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spout1
Entrez Gene ID	227695
Full Name	SPOUT domain containing methyltransferase 1
Synonyms	9930028P05,AL033358,CENP-32,D2Wsu81e
General protein information	Preferred Names SPOUT domain containing methyltransferase 1 Names putative methyltransferase C9orf114 homolog centromere protein 32 kinetochore-associated protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 B\|2 21.29 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_172660.4	NP_766248.3	putative methyltransferase C9orf114 homolog

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	C17H9ORF114	NP_001264604.1
Danio rerio (zebrafish)	zgc:101748	NP_001004627.1
Drosophila melanogaster (fruit fly)	CG12128	NP_610543.1
Caenorhabditis elegans (roundworm)	B0361.6	NP_498602.1
Magnaporthe oryzae (rice blast fungus)	MGG_02881	XP_003720850.1
Bos taurus (cattle)	C11H9orf114	XP_002691684.1
Oryza sativa (rice)	Os04g0244500	NP_001052303.1
Pan troglodytes (chimpanzee)	C9H9orf114	XP_520300.3
	LOC722998	XP_001119071.2
Canis lupus familiaris (dog)	C9H9orf114	XP_851147.2
Mus musculus (house mouse)	D2Wsu81e	NP_766248.3
Neurospora crassa	NCU00597	XP_965737.1
Arabidopsis thaliana (thale cress)	AT5G19300	NP_197431.2
Xenopus tropicalis (tropical clawed frog)	c9orf114	NP_001016729.1
Homo sapiens (human)	C9orf114	NP_057474.2
Gallus gallus (chicken)	C17H9orf114	XP_003642329.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007570	XP_001687890.1

Gene Ontology
Bibliography

Related articles in PubMed

Generation and characterization of endonuclease G null mice.
Irvine RA, Adachi N, Shibata DK, Cassell GD, Yu K, Karanjawala ZE, Hsieh CL, Lieber MR
Molecular and cellular biology25(1)294-302(2005 Jan)

Transcriptome analysis of mouse stem cells and early embryos.
Sharov AA, Piao Y, Matoba R, Dudekula DB, Qian Y, VanBuren V, Falco G, Martin PR, Stagg CA, Bassey UC, Wang Y, Carter MG, Hamatani T, Aiba K, Akutsu H, Sharova L, Tanaka TS, Kimber WL, Yoshikawa T, Jaradat SA, Pantano S, Nagaraja R, Boheler KR, Taub D, Hodes RJ, Longo DL, Schlessinger D, Keller J, Klotz E, Kelsoe G, Umezawa A, Vescovi AL, Rossant J, Kunath T, Hogan BL, Curci A, D'Urso M, Kelso J, Hide W, Ko MS
PLoS biology1(3)E74(2003 Dec)

Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome.
Ko MS, Threat TA, Wang X, Horton JH, Cui Y, Wang X, Pryor E, Paris J, Wells-Smith J, Kitchen JR, Rowe LB, Eppig J, Satoh T, Brant L, Fujiwara H, Yotsumoto S, Nakashima H
Human molecular genetics7(12)1967-78(1998 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Spout1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spout1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu03845	NM_172660.4 Latest version!	Mus musculus SPOUT domain containing methyltransferase 1 (Spout1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spout1 ( NM_172660.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766248.3 Mus musculus SPOUT domain containing methyltransferase 1 (Spout1), mRNA.

Price & Availability↑

CloneID	OMu03845
Clone ID Related Accession (Same CDS sequence)	NM_172660.4
Accession Version	NM_172660.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	putative methyltransferase C9orf114 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY289934.1, AK147162.1 and BY462424.1. On or before Sep 2, 2010 this sequence version replaced NM_001166622.1, NM_172660.3. ##Evidence-Data-START## Transcript exon combination :: AK147162.1, SRR1660817.120330.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849386 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGC GCCCGAGGAA GCGTCCGTGT GGTCCGGGTG AACATGGCCA AAGGGTTGAA 
TGGCGAAAAT GGAAACAGCA GAAAAAAGAG GAGAAAAAGA AGTGGAAGGA CCTCAAGATA 
ATGAAGAAAC TGGAGCGGCA GCGTGCCCAA GAGGAAGAGG CTAAACGCCA GGAAGAGGAG 
GAAGAGGCGG CGGCCCAGAG GAGCAACCAA GGACGGCCTT ACACTCTGAG TGTGGCCCTG 
CCAGGCTCCA TCCTGGACAA TGCCCAGTCA CCTGAGCTCC GCACCTACCT GGCTGGCCAG 
ATCGCCAGAG CCTGCACCAT CTTCTGTGTG GATGAGATTG TGGTGTTCGA TGAGGAGGGC 
CAAGATACCA AGAGTGTGGA AGGAGAATTC AGGGGAGTTG GCAAGAAAGG GCAGGCATGT 
GTCCAGCTGG CCCGCATCCT ACAGTACCTG GAGTGTCCAC AGTACCTGAG AAAGGCGTTC 
TTCCCCAAGC ACCAAGATCT GCAGTTTGCA GGAATCCTCA ACCCCTTGGA CAGCCCTCAC 
CACATGCGTC AGGACGAAGA GTCTGAGTTT CGAGAAGGCG TCGTGGTGGA CCGGCCCACC 
AAGGCAGGCC ATGGCTCTTT GGTCAACTGT GGAATGAAGA AGGAGGTGAA GATTGACAAG 
AAACTGGACC CTGGACTTCG GGTGACCGTG CGACTGAACC AGCAGCAGCT CCCAGAATGC 
AAGACATACA AAGGAACAGT TGTGTCATCA CAGGACCCCC GCACCAAAGC TGGTCTCTAC 
TGGGGCTACA CTGTCCGGCT GGCCTCTTGC CTCAGCGCTG TGTTTGCTGA GGCTCCCTTC 
CAGGATGGGT ACGACCTGAC CATTGGGACA TCAGAACGTG GCTCCGACGT GGCCTCTGCC 
CAGCTGCCCA GCTTCAGGCA TGCCCTTGTG GTGTTTGGAG GCCTCCAAGG GCTAGAAGCT 
GCAGTGGATG CTGACCCGAA CCTTGAAGTG GCTGACCCCA GTGTCCTCTT CGACTTCTAT 
GTCAACACAT GCCTTAGCCA GGGCAGCCGT ACCATCCGCA CCGAGGAGGC CATACTCATC 
TCGCTGGCTG CTCTGCAGCC TGGTCTCACC CAGGTGGGAT CGAGGCCCGC CTCCCCGCTT 
TCTGGACCCA GGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766248.3
CDS	35..1192
Translation	MAERPRKRPCGPGEHGQRVEWRKWKQQKKEEKKKWKDLKIMKKLERQRAQEEEAKRQEEEEEAAAQRSNQGRPYTLSVALPGSILDNAQSPELRTYLAGQIARACTIFCVDEIVVFDEEGQDTKSVEGEFRGVGKKGQACVQLARILQYLECPQYLRKAFFPKHQDLQFAGILNPLDSPHHMRQDEESEFREGVVVDRPTKAGHGSLVNCGMKKEVKIDKKLDPGLRVTVRLNQQQLPECKTYKGTVVSSQDPRTKAGLYWGYTVRLASCLSAVFAEAPFQDGYDLTIGTSERGSDVASAQLPSFRHALVVFGGLQGLEAAVDADPNLEVADPSVLFDFYVNTCLSQGSRTIRTEEAILISLAALQPGLTQVGSRPASPLSGPRM

Target ORF information:

RefSeq Version	NM_172660.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPOUT domain containing methyltransferase 1 (Spout1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172660.4

ORF Insert Sequence:

ATGGCGGAGC GCCCGAGGAA GCGTCCGTGT GGTCCGGGTG AACATGGCCA AAGGGTTGAA 
TGGCGAAAAT GGAAACAGCA GAAAAAAGAG GAGAAAAAGA AGTGGAAGGA CCTCAAGATA 
ATGAAGAAAC TGGAGCGGCA GCGTGCCCAA GAGGAAGAGG CTAAACGCCA GGAAGAGGAG 
GAAGAGGCGG CGGCCCAGAG GAGCAACCAA GGACGGCCTT ACACTCTGAG TGTGGCCCTG 
CCAGGCTCCA TCCTGGACAA TGCCCAGTCA CCTGAGCTCC GCACCTACCT GGCTGGCCAG 
ATCGCCAGAG CCTGCACCAT CTTCTGTGTG GATGAGATTG TGGTGTTCGA TGAGGAGGGC 
CAAGATACCA AGAGTGTGGA AGGAGAATTC AGGGGAGTTG GCAAGAAAGG GCAGGCATGT 
GTCCAGCTGG CCCGCATCCT ACAGTACCTG GAGTGTCCAC AGTACCTGAG AAAGGCGTTC 
TTCCCCAAGC ACCAAGATCT GCAGTTTGCA GGAATCCTCA ACCCCTTGGA CAGCCCTCAC 
CACATGCGTC AGGACGAAGA GTCTGAGTTT CGAGAAGGCG TCGTGGTGGA CCGGCCCACC 
AAGGCAGGCC ATGGCTCTTT GGTCAACTGT GGAATGAAGA AGGAGGTGAA GATTGACAAG 
AAACTGGACC CTGGACTTCG GGTGACCGTG CGACTGAACC AGCAGCAGCT CCCAGAATGC 
AAGACATACA AAGGAACAGT TGTGTCATCA CAGGACCCCC GCACCAAAGC TGGTCTCTAC 
TGGGGCTACA CTGTCCGGCT GGCCTCTTGC CTCAGCGCTG TGTTTGCTGA GGCTCCCTTC 
CAGGATGGGT ACGACCTGAC CATTGGGACA TCAGAACGTG GCTCCGACGT GGCCTCTGCC 
CAGCTGCCCA GCTTCAGGCA TGCCCTTGTG GTGTTTGGAG GCCTCCAAGG GCTAGAAGCT 
GCAGTGGATG CTGACCCGAA CCTTGAAGTG GCTGACCCCA GTGTCCTCTT CGACTTCTAT 
GTCAACACAT GCCTTAGCCA GGGCAGCCGT ACCATCCGCA CCGAGGAGGC CATACTCATC 
TCGCTGGCTG CTCTGCAGCC TGGTCTCACC CAGGTGGGAT CGAGGCCCGC CTCCCCGCTT 
TCTGGACCCA GGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Generation and characterization of endonuclease G null mice.
Molecular and cellular biology25(1)294-302(2005 Jan)
Irvine RA,Adachi N,Shibata DK,Cassell GD,Yu K,Karanjawala ZE,Hsieh CL,Lieber MR

Transcriptome analysis of mouse stem cells and early embryos.
PLoS biology1(3)E74(2003 Dec)
Sharov AA,Piao Y,Matoba R,Dudekula DB,Qian Y,VanBuren V,Falco G,Martin PR,Stagg CA,Bassey UC,Wang Y,Carter MG,Hamatani T,Aiba K,Akutsu H,Sharova L,Tanaka TS,Kimber WL,Yoshikawa T,Jaradat SA,Pantano S,Nagaraja R,Boheler KR,Taub D,Hodes RJ,Longo DL,Schlessinger D,Keller J,Klotz E,Kelsoe G,Umezawa A,Vescovi AL,Rossant J,Kunath T,Hogan BL,Curci A,D'Urso M,Kelso J,Hide W,Ko MS

Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome.
Human molecular genetics7(12)1967-78(1998 Nov)
Ko MS,Threat TA,Wang X,Horton JH,Cui Y,Wang X,Pryor E,Paris J,Wells-Smith J,Kitchen JR,Rowe LB,Eppig J,Satoh T,Brant L,Fujiwara H,Yotsumoto S,Nakashima H

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Spout1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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