Tk1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tk1
Entrez Gene ID	21877
Full Name	thymidine kinase 1
Synonyms	D530002A18Rik,Tk-1,Tk1a,Tk1b
General protein information	Preferred Names thymidine kinase 1 Names thymidine kinase, cytosolic
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 E2\|11 82.96 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_030245917.1	XP_030101777.1	thymidine kinase, cytosolic isoform X2
XM_006533151.3	XP_006533214.1	thymidine kinase, cytosolic isoform X3
NM_009387.2	NP_033413.2	thymidine kinase, cytosolic isoform 1
XM_006533150.3	XP_006533213.1	thymidine kinase, cytosolic isoform X2
XM_006533150.2	XP_006533213.1	thymidine kinase, cytosolic isoform X1
XM_006533149.3	XP_006533212.1	thymidine kinase, cytosolic isoform X1
NM_001271729.1	NP_001258658.1	thymidine kinase, cytosolic isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	TK1	NP_003249.3
Pan troglodytes (chimpanzee)	TK1	XP_003315799.1
Mus musculus (house mouse)	Tk1	NP_033413.2
Danio rerio (zebrafish)	LOC100537847	XP_003199583.1
Bos taurus (cattle)	TK1	NP_001091041.1
Danio rerio (zebrafish)	tk1	NP_956126.1
	TK1	XP_001107944.2
Rattus norvegicus (Norway rat)	Tk1	NP_434687.1
Caenorhabditis elegans (roundworm)	thk-1	NP_001255477.1
Arabidopsis thaliana (thale cress)	AT5G23070	NP_568426.1
Gallus gallus (chicken)	TK1	NP_990229.1
Canis lupus familiaris (dog)	TK1	XP_003639284.1
Xenopus tropicalis (tropical clawed frog)	tk1	NP_001011452.1

Gene Ontology
Bibliography

Related articles in PubMed

Activation of the STING-Dependent Type I Interferon Response Reduces Microglial Reactivity and Neuroinflammation.
Mathur V, Burai R, Vest RT, Bonanno LN, Lehallier B, Zardeneta ME, Mistry KN, Do D, Marsh SE, Abud EM, Blurton-Jones M, Li L, Lashuel HA, Wyss-Coray T
Neuron96(6)1290-1302.e6(2017 Dec)

Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development.
Naiman N, Fujioka K, Fujino M, Valerius MT, Potter SS, McMahon AP, Kobayashi A
Developmental cell41(4)349-365.e3(2017 05)

Widespread and dynamic translational control of red blood cell development.
Alvarez-Dominguez JR, Zhang X, Hu W
Blood129(5)619-629(2017 02)

Nucleoside salvage pathway kinases regulate hematopoiesis by linking nucleotide metabolism with replication stress.
Austin WR, Armijo AL, Campbell DO, Singh AS, Hsieh T, Nathanson D, Herschman HR, Phelps ME, Witte ON, Czernin J, Radu CG
The Journal of experimental medicine209(12)2215-28(2012 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The down-regulation of Tk1 activity unmasks Tk2 deficiency in Tk2-/- mice and correlates with the onset of mtDNA depletion in the brain and the heart.
Title: Onset and organ specificity of Tk2 deficiency depends on Tk1 down-regulation and transcriptional compensation.

blood cells are less dependent on mitochondrial Tk2 compared with several other tissues; these cells can synthesize deoxyribonucleotides required for mtDNA replication by alternative pathways such as phosphorylation of thymidine by cytosolic Tk1
Title: Hematopoiesis in the thymidine kinase 2 deficient mouse model of mitochondrial DNA depletion syndrome.

the small colony mutant phenotype in L5178Y lymphoma is not caused by deletion of both Trp53 and thymidine kinase 1
Title: Loss of P53 heterozygosity is not responsible for the small colony thymidine kinase mutant phenotype in L5178Y mouse lymphoma cells.

Mutations in the TK1 gene were studied in neonatal transgenic mice treated with AZT.
Title: Analysis of mutations in the Tk gene of Tk(+/-) mice treated as neonates with 3'-azido-3'-deoxythymidine (AZT).

Alterations in Tk KO mice indicate that the pyrimidine nucleotide salvage pathway is indispensable in vivo.
Title: Mice deficient for cytosolic thymidine kinase gene develop fatal kidney disease.

The following Tk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13248	NM_009387.2 Latest version!	Mus musculus thymidine kinase 1 (Tk1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu46208	XM_006533149.3 Latest version!	Mus musculus thymidine kinase 1 (Tk1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu46209	XM_006533151.3 Latest version!	Mus musculus thymidine kinase 1 (Tk1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu07279	NM_001271729.1 Latest version!	Mus musculus thymidine kinase 1 (Tk1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu07279	XM_006533150.3 Latest version!	Mus musculus thymidine kinase 1 (Tk1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu07279	XM_030245917.1 Latest version!	Mus musculus thymidine kinase 1 (Tk1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu07279	XM_006533150.2	Mus musculus thymidine kinase 1 (Tk1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tk1 ( NM_009387.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033413.2 Mus musculus thymidine kinase 1 (Tk1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13248
Clone ID Related Accession (Same CDS sequence)	NM_009387.2
Accession Version	NM_009387.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	thymidine kinase, cytosolic isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY773001.1, AK139244.1 and AA047942.1. On Apr 18, 2009 this sequence version replaced NM_009387.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: An in-frame AUG is located 23 codons upstream of the annotated translation start site but is not being annotated as a start site since it is not conserved and is in a weak Kozak sequence context. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC091758.1, AK085188.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164140 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCTACA TCAATCTGCC CACCGTGCTG CCCAGCTCCC CCAGCAAGAC TCGGGGGCAG 
ATTCAGGTGA TTCTCGGGCC CATGTTCTCA GGGAAAAGCA CAGAGCTGAT GAGAAGAGTC 
CGGCGCTTCC AGATCGCCCA GTACAAGTGC CTGGTCATCA AGTATGCCAA AGACACGCGC 
TATAGCAACA GCTTCTCCAC ACATGATCGG AACACCATGG ACGCATTGCC AGCCTGCATG 
CTCCGCGATG TGACCCAGGA GTCCTTGGGT GTGGCCGTCA TTGGCATCGA TGAGGGGCAG 
TTTTTTCCTG ACATTGTGGA TTTCTGTGAA ATGATGGCCA ACGAGGGCAA GACAGTAATT 
GTGGCAGCGC TGGATGGGAC CTTCCAGAGG AAGGCTTTCG GCAGCATCTT GAACCTGGTG 
CCCCTGGCGG AGAGTGTGGT GAAGCTCACC GCTGTGTGCA TGGAGTGCTT CCGAGAAGCT 
GCCTACACGA AGAGGCTGGG CCTGGAGAAA GAGGTGGAGG TGATTGGCGG AGCCGACAAG 
TATCACTCCG TGTGCCGCCT GTGCTACTTT AAGAAGTCTT CAGCCCAGAC TGCTGGCTCA 
GACAACAAGA ACTGTCTGGT GCTGGGGCAG CCGGGAGAGG CCTTGGTTGT CAGGAAGCTC 
TTTGCCTCTC AGCAAGTCCT ACAATACAAC TCTGCCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033413.2
CDS	236..937
Translation	MSYINLPTVLPSSPSKTRGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYSNSFSTHDRNTMDALPACMLRDVTQESLGVAVIGIDEGQFFPDIVDFCEMMANEGKTVIVAALDGTFQRKAFGSILNLVPLAESVVKLTAVCMECFREAAYTKRLGLEKEVEVIGGADKYHSVCRLCYFKKSSAQTAGSDNKNCLVLGQPGEALVVRKLFASQQVLQYNSAN

Target ORF information:

RefSeq Version	NM_009387.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thymidine kinase 1 (Tk1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009387.2

ORF Insert Sequence:

ATGAGCTACA TCAATCTGCC CACCGTGCTG CCCAGCTCCC CCAGCAAGAC TCGGGGGCAG 
ATTCAGGTGA TTCTCGGGCC CATGTTCTCA GGGAAAAGCA CAGAGCTGAT GAGAAGAGTC 
CGGCGCTTCC AGATCGCCCA GTACAAGTGC CTGGTCATCA AGTATGCCAA AGACACGCGC 
TATAGCAACA GCTTCTCCAC ACATGATCGG AACACCATGG ACGCATTGCC AGCCTGCATG 
CTCCGCGATG TGACCCAGGA GTCCTTGGGT GTGGCCGTCA TTGGCATCGA TGAGGGGCAG 
TTTTTTCCTG ACATTGTGGA TTTCTGTGAA ATGATGGCCA ACGAGGGCAA GACAGTAATT 
GTGGCAGCGC TGGATGGGAC CTTCCAGAGG AAGGCTTTCG GCAGCATCTT GAACCTGGTG 
CCCCTGGCGG AGAGTGTGGT GAAGCTCACC GCTGTGTGCA TGGAGTGCTT CCGAGAAGCT 
GCCTACACGA AGAGGCTGGG CCTGGAGAAA GAGGTGGAGG TGATTGGCGG AGCCGACAAG 
TATCACTCCG TGTGCCGCCT GTGCTACTTT AAGAAGTCTT CAGCCCAGAC TGCTGGCTCA 
GACAACAAGA ACTGTCTGGT GCTGGGGCAG CCGGGAGAGG CCTTGGTTGT CAGGAAGCTC 
TTTGCCTCTC AGCAAGTCCT ACAATACAAC TCTGCCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tk1 ( XM_006533149.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533212.1 Mus musculus thymidine kinase 1 (Tk1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu46208
Clone ID Related Accession (Same CDS sequence)	XM_006533149.3
Accession Version	XM_006533149.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	thymidine kinase, cytosolic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533149.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGCAG TAAGTCGAAA TTTTTCCACC CACGGACTCT CGGTGCTAAC TAAGGTTTGC 
ACAGCAGCCA TGAGCTACAT CAATCTGCCC ACCGTGCTGC CCAGCTCCCC CAGCAAGACT 
CGGGGGCAGA TTCAGGTGAT TCTCGGGCCC ATGTTCTCAG GGAAAAGCAC AGAGCTGATG 
AGAAGAGTCC GGCGCTTCCA GATCGCCCAG TACAAGTGCC TGGTCATCAA GTATGCCAAA 
GACACGCGCT ATAGCAACAG CTTCTCCACA CATGATCGGA ACACCATGGA CGCATTGCCA 
GCCTGCATGC TCCGCGATGT GACCCAGGAG TCCTTGGGTG TGGCCGTCAT TGGCATCGAT 
GAGGGGCAGT TTTTTCCTGA CATTGTGGAT TTCTGTGAAA TGATGGCCAA CGAGGGCAAG 
ACAGTAATTG TGGCAGCGCT GGATGGGACC TTCCAGAGGA AGGCTTTCGG CAGCATCTTG 
AACCTGGTGC CCCTGGCGGA GAGTGTGGTG AAGCTCACCG CTGTGTGCAT GGAGTGCTTC 
CGAGAAGCTG CCTACACGAA GAGGCTGGGC CTGGAGAAAG AGGTGGAGGT GATTGGGCCT 
GGAGAAAGAG GTATACACAC ACACACACAC ACACACACAC ACACTTCTGC CCAAGGAGTG 
GGGTGGGCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533212.1
CDS	36..707
Translation	MRAVSRNFSTHGLSVLTKVCTAAMSYINLPTVLPSSPSKTRGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYSNSFSTHDRNTMDALPACMLRDVTQESLGVAVIGIDEGQFFPDIVDFCEMMANEGKTVIVAALDGTFQRKAFGSILNLVPLAESVVKLTAVCMECFREAAYTKRLGLEKEVEVIGPGERGIHTHTHTHTHTSAQGVGWA

Target ORF information:

RefSeq Version	XM_006533149.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thymidine kinase 1 (Tk1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533149.3

ORF Insert Sequence:

ATGCGAGCAG TAAGTCGAAA TTTTTCCACC CACGGACTCT CGGTGCTAAC TAAGGTTTGC 
ACAGCAGCCA TGAGCTACAT CAATCTGCCC ACCGTGCTGC CCAGCTCCCC CAGCAAGACT 
CGGGGGCAGA TTCAGGTGAT TCTCGGGCCC ATGTTCTCAG GGAAAAGCAC AGAGCTGATG 
AGAAGAGTCC GGCGCTTCCA GATCGCCCAG TACAAGTGCC TGGTCATCAA GTATGCCAAA 
GACACGCGCT ATAGCAACAG CTTCTCCACA CATGATCGGA ACACCATGGA CGCATTGCCA 
GCCTGCATGC TCCGCGATGT GACCCAGGAG TCCTTGGGTG TGGCCGTCAT TGGCATCGAT 
GAGGGGCAGT TTTTTCCTGA CATTGTGGAT TTCTGTGAAA TGATGGCCAA CGAGGGCAAG 
ACAGTAATTG TGGCAGCGCT GGATGGGACC TTCCAGAGGA AGGCTTTCGG CAGCATCTTG 
AACCTGGTGC CCCTGGCGGA GAGTGTGGTG AAGCTCACCG CTGTGTGCAT GGAGTGCTTC 
CGAGAAGCTG CCTACACGAA GAGGCTGGGC CTGGAGAAAG AGGTGGAGGT GATTGGGCCT 
GGAGAAAGAG GTATACACAC ACACACACAC ACACACACAC ACACTTCTGC CCAAGGAGTG 
GGGTGGGCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tk1 ( XM_006533151.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533214.1 Mus musculus thymidine kinase 1 (Tk1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu46209
Clone ID Related Accession (Same CDS sequence)	XM_006533151.3
Accession Version	XM_006533151.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	thymidine kinase, cytosolic isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533151.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGCAG TAAGTCGAAA TTTTTCCACC CACGGACTCT CGGTGCTAAC TAAGGTTTGC 
ACAGCAGCCA TGAGCTACAT CAATCTGCCC ACCGTGCTGC CCAGCTCCCC CAGCAAGACT 
CGGGGGCAGA TTCAGGTGAT TCTCGGGCCC ATGTTCTCAG GGAAAAGCAC AGAGCTGATG 
AGAAGAGTCC GGCGCTTCCA GATCGCCCAG TACAAGTGCC TGGTCATCAA GTATGCCAAA 
GACACGCGCT ATAGCAACAG CTTCTCCACA CATGATCGGA ACACCATGGA CGCATTGCCA 
GCCTGCATGC TCCGCGATGT GACCCAGGAG TCCTTGGGTG TGGCCGTCAT TGGCATCGAT 
GAGGGGCAGT TTGCTGTCTC CAGTCCACCA GGAAGAGCAA GGACAGAGGC CTCTAACACT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533214.1
CDS	240..662
Translation	MRAVSRNFSTHGLSVLTKVCTAAMSYINLPTVLPSSPSKTRGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYSNSFSTHDRNTMDALPACMLRDVTQESLGVAVIGIDEGQFAVSSPPGRARTEASNT

Target ORF information:

RefSeq Version	XM_006533151.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thymidine kinase 1 (Tk1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533151.3

ORF Insert Sequence:

ATGCGAGCAG TAAGTCGAAA TTTTTCCACC CACGGACTCT CGGTGCTAAC TAAGGTTTGC 
ACAGCAGCCA TGAGCTACAT CAATCTGCCC ACCGTGCTGC CCAGCTCCCC CAGCAAGACT 
CGGGGGCAGA TTCAGGTGAT TCTCGGGCCC ATGTTCTCAG GGAAAAGCAC AGAGCTGATG 
AGAAGAGTCC GGCGCTTCCA GATCGCCCAG TACAAGTGCC TGGTCATCAA GTATGCCAAA 
GACACGCGCT ATAGCAACAG CTTCTCCACA CATGATCGGA ACACCATGGA CGCATTGCCA 
GCCTGCATGC TCCGCGATGT GACCCAGGAG TCCTTGGGTG TGGCCGTCAT TGGCATCGAT 
GAGGGGCAGT TTGCTGTCTC CAGTCCACCA GGAAGAGCAA GGACAGAGGC CTCTAACACT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tk1 ( NM_001271729.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001258658.1 Mus musculus thymidine kinase 1 (Tk1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu07279
Clone ID Related Accession (Same CDS sequence)	XM_030245917.1 , XM_006533150.3 , XM_006533150.2 , NM_001271729.1
Accession Version	NM_001271729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	thymidine kinase, cytosolic isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY773001.1, CF732600.1, AK139244.1 and AA047942.1. Transcript Variant: This variant (2) lacks an alternate coding exon compared to variant 1, that causes a frameshift and use of a downstream translation start site compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI408754.1, CF732600.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAAGAG TCCGGCGCTT CCAGATCGCC CAGTACAAGT GCCTGGTCAT CAAGTATGCC 
AAAGACACGC GCTATAGCAA CAGCTTCTCC ACACATGATC GGAACACCAT GGACGCATTG 
CCAGCCTGCA TGCTCCGCGA TGTGACCCAG GAGTCCTTGG GTGTGGCCGT CATTGGCATC 
GATGAGGGGC AGTTTTTTCC TGACATTGTG GATTTCTGTG AAATGATGGC CAACGAGGGC 
AAGACAGTAA TTGTGGCAGC GCTGGATGGG ACCTTCCAGA GGAAGGCTTT CGGCAGCATC 
TTGAACCTGG TGCCCCTGGC GGAGAGTGTG GTGAAGCTCA CCGCTGTGTG CATGGAGTGC 
TTCCGAGAAG CTGCCTACAC GAAGAGGCTG GGCCTGGAGA AAGAGGTGGA GGTGATTGGC 
GGAGCCGACA AGTATCACTC CGTGTGCCGC CTGTGCTACT TTAAGAAGTC TTCAGCCCAG 
ACTGCTGGCT CAGACAACAA GAACTGTCTG GTGCTGGGGC AGCCGGGAGA GGCCTTGGTT 
GTCAGGAAGC TCTTTGCCTC TCAGCAAGTC CTACAATACA ACTCTGCCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258658.1
CDS	312..905
Translation	MRRVRRFQIAQYKCLVIKYAKDTRYSNSFSTHDRNTMDALPACMLRDVTQESLGVAVIGIDEGQFFPDIVDFCEMMANEGKTVIVAALDGTFQRKAFGSILNLVPLAESVVKLTAVCMECFREAAYTKRLGLEKEVEVIGGADKYHSVCRLCYFKKSSAQTAGSDNKNCLVLGQPGEALVVRKLFASQQVLQYNSAN

Target ORF information:

RefSeq Version	NM_001271729.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thymidine kinase 1 (Tk1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271729.1

ORF Insert Sequence:

ATGAGAAGAG TCCGGCGCTT CCAGATCGCC CAGTACAAGT GCCTGGTCAT CAAGTATGCC 
AAAGACACGC GCTATAGCAA CAGCTTCTCC ACACATGATC GGAACACCAT GGACGCATTG 
CCAGCCTGCA TGCTCCGCGA TGTGACCCAG GAGTCCTTGG GTGTGGCCGT CATTGGCATC 
GATGAGGGGC AGTTTTTTCC TGACATTGTG GATTTCTGTG AAATGATGGC CAACGAGGGC 
AAGACAGTAA TTGTGGCAGC GCTGGATGGG ACCTTCCAGA GGAAGGCTTT CGGCAGCATC 
TTGAACCTGG TGCCCCTGGC GGAGAGTGTG GTGAAGCTCA CCGCTGTGTG CATGGAGTGC 
TTCCGAGAAG CTGCCTACAC GAAGAGGCTG GGCCTGGAGA AAGAGGTGGA GGTGATTGGC 
GGAGCCGACA AGTATCACTC CGTGTGCCGC CTGTGCTACT TTAAGAAGTC TTCAGCCCAG 
ACTGCTGGCT CAGACAACAA GAACTGTCTG GTGCTGGGGC AGCCGGGAGA GGCCTTGGTT 
GTCAGGAAGC TCTTTGCCTC TCAGCAAGTC CTACAATACA ACTCTGCCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tk1 ( XM_030245917.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101777.1 Mus musculus thymidine kinase 1 (Tk1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu07279
Clone ID Related Accession (Same CDS sequence)	XM_030245917.1 , XM_006533150.3 , XM_006533150.2 , NM_001271729.1
Accession Version	XM_030245917.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	thymidine kinase, cytosolic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGAG TCCGGCGCTT CCAGATCGCC CAGTACAAGT GCCTGGTCAT CAAGTATGCC 
AAAGACACGC GCTATAGCAA CAGCTTCTCC ACACATGATC GGAACACCAT GGACGCATTG 
CCAGCCTGCA TGCTCCGCGA TGTGACCCAG GAGTCCTTGG GTGTGGCCGT CATTGGCATC 
GATGAGGGGC AGTTTTTTCC TGACATTGTG GATTTCTGTG AAATGATGGC CAACGAGGGC 
AAGACAGTAA TTGTGGCAGC GCTGGATGGG ACCTTCCAGA GGAAGGCTTT CGGCAGCATC 
TTGAACCTGG TGCCCCTGGC GGAGAGTGTG GTGAAGCTCA CCGCTGTGTG CATGGAGTGC 
TTCCGAGAAG CTGCCTACAC GAAGAGGCTG GGCCTGGAGA AAGAGGTGGA GGTGATTGGC 
GGAGCCGACA AGTATCACTC CGTGTGCCGC CTGTGCTACT TTAAGAAGTC TTCAGCCCAG 
ACTGCTGGCT CAGACAACAA GAACTGTCTG GTGCTGGGGC AGCCGGGAGA GGCCTTGGTT 
GTCAGGAAGC TCTTTGCCTC TCAGCAAGTC CTACAATACA ACTCTGCCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101777.1
CDS	101..694
Translation	MRRVRRFQIAQYKCLVIKYAKDTRYSNSFSTHDRNTMDALPACMLRDVTQESLGVAVIGIDEGQFFPDIVDFCEMMANEGKTVIVAALDGTFQRKAFGSILNLVPLAESVVKLTAVCMECFREAAYTKRLGLEKEVEVIGGADKYHSVCRLCYFKKSSAQTAGSDNKNCLVLGQPGEALVVRKLFASQQVLQYNSAN

Target ORF information:

RefSeq Version	XM_030245917.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thymidine kinase 1 (Tk1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245917.1

ORF Insert Sequence:

ATGAGAAGAG TCCGGCGCTT CCAGATCGCC CAGTACAAGT GCCTGGTCAT CAAGTATGCC 
AAAGACACGC GCTATAGCAA CAGCTTCTCC ACACATGATC GGAACACCAT GGACGCATTG 
CCAGCCTGCA TGCTCCGCGA TGTGACCCAG GAGTCCTTGG GTGTGGCCGT CATTGGCATC 
GATGAGGGGC AGTTTTTTCC TGACATTGTG GATTTCTGTG AAATGATGGC CAACGAGGGC 
AAGACAGTAA TTGTGGCAGC GCTGGATGGG ACCTTCCAGA GGAAGGCTTT CGGCAGCATC 
TTGAACCTGG TGCCCCTGGC GGAGAGTGTG GTGAAGCTCA CCGCTGTGTG CATGGAGTGC 
TTCCGAGAAG CTGCCTACAC GAAGAGGCTG GGCCTGGAGA AAGAGGTGGA GGTGATTGGC 
GGAGCCGACA AGTATCACTC CGTGTGCCGC CTGTGCTACT TTAAGAAGTC TTCAGCCCAG 
ACTGCTGGCT CAGACAACAA GAACTGTCTG GTGCTGGGGC AGCCGGGAGA GGCCTTGGTT 
GTCAGGAAGC TCTTTGCCTC TCAGCAAGTC CTACAATACA ACTCTGCCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tk1 ( XM_006533150.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533213.1 Mus musculus thymidine kinase 1 (Tk1), transcript variant X2, mRNA.

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CloneID	OMu07279
Clone ID Related Accession (Same CDS sequence)	XM_030245917.1 , XM_006533150.3 , XM_006533150.2 , NM_001271729.1
Accession Version	XM_006533150.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	thymidine kinase, cytosolic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533150.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGAG TCCGGCGCTT CCAGATCGCC CAGTACAAGT GCCTGGTCAT CAAGTATGCC 
AAAGACACGC GCTATAGCAA CAGCTTCTCC ACACATGATC GGAACACCAT GGACGCATTG 
CCAGCCTGCA TGCTCCGCGA TGTGACCCAG GAGTCCTTGG GTGTGGCCGT CATTGGCATC 
GATGAGGGGC AGTTTTTTCC TGACATTGTG GATTTCTGTG AAATGATGGC CAACGAGGGC 
AAGACAGTAA TTGTGGCAGC GCTGGATGGG ACCTTCCAGA GGAAGGCTTT CGGCAGCATC 
TTGAACCTGG TGCCCCTGGC GGAGAGTGTG GTGAAGCTCA CCGCTGTGTG CATGGAGTGC 
TTCCGAGAAG CTGCCTACAC GAAGAGGCTG GGCCTGGAGA AAGAGGTGGA GGTGATTGGC 
GGAGCCGACA AGTATCACTC CGTGTGCCGC CTGTGCTACT TTAAGAAGTC TTCAGCCCAG 
ACTGCTGGCT CAGACAACAA GAACTGTCTG GTGCTGGGGC AGCCGGGAGA GGCCTTGGTT 
GTCAGGAAGC TCTTTGCCTC TCAGCAAGTC CTACAATACA ACTCTGCCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533213.1
CDS	529..1122
Translation	MRRVRRFQIAQYKCLVIKYAKDTRYSNSFSTHDRNTMDALPACMLRDVTQESLGVAVIGIDEGQFFPDIVDFCEMMANEGKTVIVAALDGTFQRKAFGSILNLVPLAESVVKLTAVCMECFREAAYTKRLGLEKEVEVIGGADKYHSVCRLCYFKKSSAQTAGSDNKNCLVLGQPGEALVVRKLFASQQVLQYNSAN

Target ORF information:

RefSeq Version	XM_006533150.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus thymidine kinase 1 (Tk1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533150.3

ORF Insert Sequence:

ATGAGAAGAG TCCGGCGCTT CCAGATCGCC CAGTACAAGT GCCTGGTCAT CAAGTATGCC 
AAAGACACGC GCTATAGCAA CAGCTTCTCC ACACATGATC GGAACACCAT GGACGCATTG 
CCAGCCTGCA TGCTCCGCGA TGTGACCCAG GAGTCCTTGG GTGTGGCCGT CATTGGCATC 
GATGAGGGGC AGTTTTTTCC TGACATTGTG GATTTCTGTG AAATGATGGC CAACGAGGGC 
AAGACAGTAA TTGTGGCAGC GCTGGATGGG ACCTTCCAGA GGAAGGCTTT CGGCAGCATC 
TTGAACCTGG TGCCCCTGGC GGAGAGTGTG GTGAAGCTCA CCGCTGTGTG CATGGAGTGC 
TTCCGAGAAG CTGCCTACAC GAAGAGGCTG GGCCTGGAGA AAGAGGTGGA GGTGATTGGC 
GGAGCCGACA AGTATCACTC CGTGTGCCGC CTGTGCTACT TTAAGAAGTC TTCAGCCCAG 
ACTGCTGGCT CAGACAACAA GAACTGTCTG GTGCTGGGGC AGCCGGGAGA GGCCTTGGTT 
GTCAGGAAGC TCTTTGCCTC TCAGCAAGTC CTACAATACA ACTCTGCCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tk1 ( XM_006533150.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533213.1 Mus musculus thymidine kinase 1 (Tk1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu07279
Clone ID Related Accession (Same CDS sequence)	XM_030245917.1 , XM_006533150.3 , XM_006533150.2 , NM_001271729.1
Accession Version	XM_006533150.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	thymidine kinase, cytosolic isoform X1
Comment

ATGAGAAGAG TCCGGCGCTT CCAGATCGCC CAGTACAAGT GCCTGGTCAT CAAGTATGCC 
AAAGACACGC GCTATAGCAA CAGCTTCTCC ACACATGATC GGAACACCAT GGACGCATTG 
CCAGCCTGCA TGCTCCGCGA TGTGACCCAG GAGTCCTTGG GTGTGGCCGT CATTGGCATC 
GATGAGGGGC AGTTTTTTCC TGACATTGTG GATTTCTGTG AAATGATGGC CAACGAGGGC 
AAGACAGTAA TTGTGGCAGC GCTGGATGGG ACCTTCCAGA GGAAGGCTTT CGGCAGCATC 
TTGAACCTGG TGCCCCTGGC GGAGAGTGTG GTGAAGCTCA CCGCTGTGTG CATGGAGTGC 
TTCCGAGAAG CTGCCTACAC GAAGAGGCTG GGCCTGGAGA AAGAGGTGGA GGTGATTGGC 
GGAGCCGACA AGTATCACTC CGTGTGCCGC CTGTGCTACT TTAAGAAGTC TTCAGCCCAG 
ACTGCTGGCT CAGACAACAA GAACTGTCTG GTGCTGGGGC AGCCGGGAGA GGCCTTGGTT 
GTCAGGAAGC TCTTTGCCTC TCAGCAAGTC CTACAATACA ACTCTGCCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533213.1
Translation	MRRVRRFQIAQYKCLVIKYAKDTRYSNSFSTHDRNTMDALPACMLRDVTQESLGVAVIGIDEGQFFPDIVDFCEMMANEGKTVIVAALDGTFQRKAFGSILNLVPLAESVVKLTAVCMECFREAAYTKRLGLEKEVEVIGGADKYHSVCRLCYFKKSSAQTAGSDNKNCLVLGQPGEALVVRKLFASQQVLQYNSAN

Target ORF information:

RefSeq Version	XM_006533150.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus thymidine kinase 1 (Tk1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533150.2

ORF Insert Sequence:

ATGAGAAGAG TCCGGCGCTT CCAGATCGCC CAGTACAAGT GCCTGGTCAT CAAGTATGCC 
AAAGACACGC GCTATAGCAA CAGCTTCTCC ACACATGATC GGAACACCAT GGACGCATTG 
CCAGCCTGCA TGCTCCGCGA TGTGACCCAG GAGTCCTTGG GTGTGGCCGT CATTGGCATC 
GATGAGGGGC AGTTTTTTCC TGACATTGTG GATTTCTGTG AAATGATGGC CAACGAGGGC 
AAGACAGTAA TTGTGGCAGC GCTGGATGGG ACCTTCCAGA GGAAGGCTTT CGGCAGCATC 
TTGAACCTGG TGCCCCTGGC GGAGAGTGTG GTGAAGCTCA CCGCTGTGTG CATGGAGTGC 
TTCCGAGAAG CTGCCTACAC GAAGAGGCTG GGCCTGGAGA AAGAGGTGGA GGTGATTGGC 
GGAGCCGACA AGTATCACTC CGTGTGCCGC CTGTGCTACT TTAAGAAGTC TTCAGCCCAG 
ACTGCTGGCT CAGACAACAA GAACTGTCTG GTGCTGGGGC AGCCGGGAGA GGCCTTGGTT 
GTCAGGAAGC TCTTTGCCTC TCAGCAAGTC CTACAATACA ACTCTGCCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Activation of the STING-Dependent Type I Interferon Response Reduces Microglial Reactivity and Neuroinflammation.
Neuron96(6)1290-1302.e6(2017 Dec)
Mathur V,Burai R,Vest RT,Bonanno LN,Lehallier B,Zardeneta ME,Mistry KN,Do D,Marsh SE,Abud EM,Blurton-Jones M,Li L,Lashuel HA,Wyss-Coray T

Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development.
Developmental cell41(4)349-365.e3(2017 05)
Naiman N,Fujioka K,Fujino M,Valerius MT,Potter SS,McMahon AP,Kobayashi A

Widespread and dynamic translational control of red blood cell development.
Blood129(5)619-629(2017 02)
Alvarez-Dominguez JR,Zhang X,Hu W

Nucleoside salvage pathway kinases regulate hematopoiesis by linking nucleotide metabolism with replication stress.
The Journal of experimental medicine209(12)2215-28(2012 Nov)
Austin WR,Armijo AL,Campbell DO,Singh AS,Hsieh T,Nathanson D,Herschman HR,Phelps ME,Witte ON,Czernin J,Radu CG

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Tk1 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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