Tmem259 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tmem259
Entrez Gene ID	216157
Full Name	transmembrane protein 259
Synonyms	AC087114.7gm2,ORF61,R32184_3,mFLJ00277
General protein information	Preferred Names transmembrane protein 259 Names membralin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 C1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006513490.1	XP_006513553.1	membralin isoform X1
XM_006513491.1	XP_006513554.1	membralin isoform X1
NM_001003949.4	NP_001003949.2	membralin isoform 2
NM_001359632.1	NP_001346561.1	membralin isoform 1
NM_001003949.3	NP_001003949.2	membralin

Pathways from BioSystems

Homologies

Homo sapiens (human)	TMEM259	NP_001028198.1
Pan troglodytes (chimpanzee)	C19H19orf6	XP_003954544.1
Xenopus tropicalis (tropical clawed frog)	tmem259	NP_001072254.1
Bos taurus (cattle)	TMEM259	XP_002689215.2
Danio rerio (zebrafish)	tmem259	XP_700127.3
Canis lupus familiaris (dog)	C20H19orf6	XP_533963.2
Gallus gallus (chicken)	C19ORF6	XP_418222.2
Mus musculus (house mouse)	Tmem259	NP_001003949.2
Rattus norvegicus (Norway rat)	Mbrl	NP_001094356.1

Gene Ontology
Bibliography

Related articles in PubMed

ER-associated degradation regulates Alzheimer's amyloid pathology and memory function by modulating ?-secretase activity.
Zhu B, Jiang L, Huang T, Zhao Y, Liu T, Zhong Y, Li X, Campos A, Pomeroy K, Masliah E, Zhang D, Xu H
Nature communications8(1)1472(2017 Nov)

The critical role of membralin in postnatal motor neuron survival and disease.
Yang B, Qu M, Wang R, Chatterton JE, Liu XB, Zhu B, Narisawa S, Millan JL, Nakanishi N, Swoboda K, Lipton SA, Zhang D
eLife4(2015 May)

Characterization and expression of the gene encoding membralin, an evolutionary conserved protein expressed in the central nervous system.
Andersson O, von Euler G
Brain research. Gene expression patterns1(3-4)205-12(2002 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Membralin plays a critical role in motor neuron survival, suggesting a novel mechanism for early-onset motor neuron disease.
Title: The critical role of membralin in postnatal motor neuron survival and disease.

genomic organization and expression (membralin)
Title: Characterization and expression of the gene encoding membralin, an evolutionary conserved protein expressed in the central nervous system.

The following Tmem259 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tmem259 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14880	NM_001003949.4 Latest version!	Mus musculus transmembrane protein 259 (Tmem259), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu44388	NM_001359632.1 Latest version!	Mus musculus transmembrane protein 259 (Tmem259), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu44388	XM_006513490.1 Latest version!	Mus musculus transmembrane protein 259 (Tmem259), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu44389	XM_006513491.1 Latest version!	Mus musculus transmembrane protein 259 (Tmem259), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
View Old Accession Versions >>
OMu14880	NM_001003949.3	Mus musculus transmembrane protein 259 (Tmem259), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tmem259 ( NM_001003949.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001003949.2 Mus musculus transmembrane protein 259 (Tmem259), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu14880
Clone ID Related Accession (Same CDS sequence)	NM_001003949.4 , NM_001003949.3
Accession Version	NM_001003949.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	membralin isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC151846.3, BQ572278.1, CX205815.1, BQ571954.1, CO044817.1 and BE947335.1. On Jan 13, 2018 this sequence version replaced NM_001003949.3. ##Evidence-Data-START## Transcript exon combination :: AY096036.1, SRR1660813.481677.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAGC ACGCGGCAGC CCCAGGGCCC GGGCCCAACG GCGGCGGGGG TGGTGGCGCG 
GCGCCCGTGC GCGGCCCTCG CGGCCCCAAT CTCAACCCCA ACCCGCTCAT CAACGTGCGC 
GACCGGCTCT TCCACGCTCT CTTCTTCAAG ATGGCCGTCA CCTACTCACG CCTCTTCCCA 
CCTGCTTTCC GCCGCCTCTT TGAATTCTTC GTTCTGCTCA AGGCCCTGTT TGTGCTTTTC 
GTCCTCGCCT ACATACACAT CGTCTTCTCC CGGTCCCCCA TCAACTGCTT AGAGCATGTT 
CGAGATCGAT GGCCGCGGGA GGGTGTCCTG CGGGTGGAGG TGCGCCACAA CTCGAGCCGG 
GCACCGGTGA TCCTGCAGTT CTGTGATGGG GGCCTCGGTG GCCTGGAGCT GGAACCCGGG 
GGCCTGGAGC TGGAGGAGGA GGAGCTCACA GTGGAGATGT TCACCAACAG CTCCATCAAG 
TTTGAGCTGG ACATTGAGCC CAAGGTGTTC AAGCCACAGA GCGGTGCAGA TGCCCTGAAC 
GACAGCCAGG ACTTCCCTTT TCCTGAGACG CCAGCAAAAG TGTGGCCACA GGATGAGTAC 
ATTGTGGAGT ACTCACTGGA ATATGGCTTC CTGCGGCTAT CCCAAGCCAC ACGCCAGCGT 
CTGAGCATTC CTGTCATGGT GGTCACCCTA GACCCCACGC GGGACCAGTG CTTTGGGGAC 
CGCTTCAGCC GCCTATTGCT GGATGAGTTC CTGGGCTATG ATGACATCCT CATGTCCAGT 
GTAAAGGGTC TGGCAGAGAA CGAGGAGAAC AAAGGCTTCT TGAGGAATGT GGTCTCTGGG 
GAGCACTACC GCTTTGTCAG CATGTGGATG GCGCGCACAT CCTACCTGGC GGCCTTTGTC 
ATCATGGTCA TCTTTACCCT CAGCGTGTCC ATGCTGTTGC GATACTCGCA CCACCAGATC 
TTCGTCTTCA TCGTGGACCT GCTGCAGATG CTGGAGATGA ACATGGCCAT CGCCTTCCCC 
GCAGCGCCCT TGCTGACCGT CATCCTGGCT CTCGTCGGGA TGGAAGCCAT CATGTCTGAG 
TTCTTCAATG ATACCACCAC GGCCTTCTAC ATCATCCTCA CTGTGTGGCT GGCCGACCAG 
TATGATGCCA TCTGCTGCCA CACCAACACC AGCAAGCGGC ACTGGCTGAG GTTCTTCTAC 
CTCTACCACT TCGCCTTCTA TGCCTACCAC TACCGCTTTA ACGGGCAGTA CAGCAGCCTG 
GCCCTGGTCA CCTCCTGGCT CTTCATCCAG CATTCCATGA TCTACTTCTT CCACCACTAT 
GAGTTGCCCG CCATCCTGCA GCAGATCCGA ATCCAGGAGA TGCTGCTTCA GACGCCACCG 
CTGGGCCCCG GGACCCCCAC GGCGCTGCCT GACGACCTCA ACAACAACTC TGGCTCCCCT 
GCCACTCCGG ATCCCAGCCC TCCCCTCGCG CTGGGCCCCA GCTCCAGCCC CGCGCCCACT 
GGCGGGGCAT CTGGGCCTGG CTCACTGGGC GCTGGGGCCT CAGTATCCGG CAGTGACCTA 
GGTTGGGTGG CCGAGACCGC CGCCATCATC TCTGACGCAT CCTTCCTGTC GGGGCTGAGC 
GCCTCTCTCC TGGAGCGGCG GCCAACAGCC CCTAGTACCC CGGACAGCTC ACGACCTGAC 
CCTGGGGTCC CTCTGGAGGA CGCACCCGCC CCTGCCGGGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003949.2
CDS	130..1854
Translation	MSEHAAAPGPGPNGGGGGGAAPVRGPRGPNLNPNPLINVRDRLFHALFFKMAVTYSRLFPPAFRRLFEFFVLLKALFVLFVLAYIHIVFSRSPINCLEHVRDRWPREGVLRVEVRHNSSRAPVILQFCDGGLGGLELEPGGLELEEEELTVEMFTNSSIKFELDIEPKVFKPQSGADALNDSQDFPFPETPAKVWPQDEYIVEYSLEYGFLRLSQATRQRLSIPVMVVTLDPTRDQCFGDRFSRLLLDEFLGYDDILMSSVKGLAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIVDLLQMLEMNMAIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILTVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRIQEMLLQTPPLGPGTPTALPDDLNNNSGSPATPDPSPPLALGPSSSPAPTGGASGPGSLGAGASVSGSDLGWVAETAAIISDASFLSGLSASLLERRPTAPSTPDSSRPDPGVPLEDAPAPAGS

Target ORF information:

RefSeq Version	NM_001003949.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protein 259 (Tmem259), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003949.4

ORF Insert Sequence:

ATGTCGGAGC ACGCGGCAGC CCCAGGGCCC GGGCCCAACG GCGGCGGGGG TGGTGGCGCG 
GCGCCCGTGC GCGGCCCTCG CGGCCCCAAT CTCAACCCCA ACCCGCTCAT CAACGTGCGC 
GACCGGCTCT TCCACGCTCT CTTCTTCAAG ATGGCCGTCA CCTACTCACG CCTCTTCCCA 
CCTGCTTTCC GCCGCCTCTT TGAATTCTTC GTTCTGCTCA AGGCCCTGTT TGTGCTTTTC 
GTCCTCGCCT ACATACACAT CGTCTTCTCC CGGTCCCCCA TCAACTGCTT AGAGCATGTT 
CGAGATCGAT GGCCGCGGGA GGGTGTCCTG CGGGTGGAGG TGCGCCACAA CTCGAGCCGG 
GCACCGGTGA TCCTGCAGTT CTGTGATGGG GGCCTCGGTG GCCTGGAGCT GGAACCCGGG 
GGCCTGGAGC TGGAGGAGGA GGAGCTCACA GTGGAGATGT TCACCAACAG CTCCATCAAG 
TTTGAGCTGG ACATTGAGCC CAAGGTGTTC AAGCCACAGA GCGGTGCAGA TGCCCTGAAC 
GACAGCCAGG ACTTCCCTTT TCCTGAGACG CCAGCAAAAG TGTGGCCACA GGATGAGTAC 
ATTGTGGAGT ACTCACTGGA ATATGGCTTC CTGCGGCTAT CCCAAGCCAC ACGCCAGCGT 
CTGAGCATTC CTGTCATGGT GGTCACCCTA GACCCCACGC GGGACCAGTG CTTTGGGGAC 
CGCTTCAGCC GCCTATTGCT GGATGAGTTC CTGGGCTATG ATGACATCCT CATGTCCAGT 
GTAAAGGGTC TGGCAGAGAA CGAGGAGAAC AAAGGCTTCT TGAGGAATGT GGTCTCTGGG 
GAGCACTACC GCTTTGTCAG CATGTGGATG GCGCGCACAT CCTACCTGGC GGCCTTTGTC 
ATCATGGTCA TCTTTACCCT CAGCGTGTCC ATGCTGTTGC GATACTCGCA CCACCAGATC 
TTCGTCTTCA TCGTGGACCT GCTGCAGATG CTGGAGATGA ACATGGCCAT CGCCTTCCCC 
GCAGCGCCCT TGCTGACCGT CATCCTGGCT CTCGTCGGGA TGGAAGCCAT CATGTCTGAG 
TTCTTCAATG ATACCACCAC GGCCTTCTAC ATCATCCTCA CTGTGTGGCT GGCCGACCAG 
TATGATGCCA TCTGCTGCCA CACCAACACC AGCAAGCGGC ACTGGCTGAG GTTCTTCTAC 
CTCTACCACT TCGCCTTCTA TGCCTACCAC TACCGCTTTA ACGGGCAGTA CAGCAGCCTG 
GCCCTGGTCA CCTCCTGGCT CTTCATCCAG CATTCCATGA TCTACTTCTT CCACCACTAT 
GAGTTGCCCG CCATCCTGCA GCAGATCCGA ATCCAGGAGA TGCTGCTTCA GACGCCACCG 
CTGGGCCCCG GGACCCCCAC GGCGCTGCCT GACGACCTCA ACAACAACTC TGGCTCCCCT 
GCCACTCCGG ATCCCAGCCC TCCCCTCGCG CTGGGCCCCA GCTCCAGCCC CGCGCCCACT 
GGCGGGGCAT CTGGGCCTGG CTCACTGGGC GCTGGGGCCT CAGTATCCGG CAGTGACCTA 
GGTTGGGTGG CCGAGACCGC CGCCATCATC TCTGACGCAT CCTTCCTGTC GGGGCTGAGC 
GCCTCTCTCC TGGAGCGGCG GCCAACAGCC CCTAGTACCC CGGACAGCTC ACGACCTGAC 
CCTGGGGTCC CTCTGGAGGA CGCACCCGCC CCTGCCGGGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmem259 ( NM_001003949.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001003949.2 Mus musculus transmembrane protein 259 (Tmem259), mRNA.

Price & Availability↑

CloneID	OMu14880
Clone ID Related Accession (Same CDS sequence)	NM_001003949.4 , NM_001003949.3
Accession Version	NM_001003949.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-31
Organism	Mus musculus(house mouse)
Product	membralin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB645384.1 and BC052787.1. On Sep 6, 2006 this sequence version replaced NM_001003949.2. ##Evidence-Data-START## Transcript exon combination :: AY096036.1, SRR1660813.481677.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGAGC ACGCGGCAGC CCCAGGGCCC GGGCCCAACG GCGGCGGGGG TGGTGGCGCG 
GCGCCCGTGC GCGGCCCTCG CGGCCCCAAT CTCAACCCCA ACCCGCTCAT CAACGTGCGC 
GACCGGCTCT TCCACGCTCT CTTCTTCAAG ATGGCCGTCA CCTACTCACG CCTCTTCCCA 
CCTGCTTTCC GCCGCCTCTT TGAATTCTTC GTTCTGCTCA AGGCCCTGTT TGTGCTTTTC 
GTCCTCGCCT ACATACACAT CGTCTTCTCC CGGTCCCCCA TCAACTGCTT AGAGCATGTT 
CGAGATCGAT GGCCGCGGGA GGGTGTCCTG CGGGTGGAGG TGCGCCACAA CTCGAGCCGG 
GCACCGGTGA TCCTGCAGTT CTGTGATGGG GGCCTCGGTG GCCTGGAGCT GGAACCCGGG 
GGCCTGGAGC TGGAGGAGGA GGAGCTCACA GTGGAGATGT TCACCAACAG CTCCATCAAG 
TTTGAGCTGG ACATTGAGCC CAAGGTGTTC AAGCCACAGA GCGGTGCAGA TGCCCTGAAC 
GACAGCCAGG ACTTCCCTTT TCCTGAGACG CCAGCAAAAG TGTGGCCACA GGATGAGTAC 
ATTGTGGAGT ACTCACTGGA ATATGGCTTC CTGCGGCTAT CCCAAGCCAC ACGCCAGCGT 
CTGAGCATTC CTGTCATGGT GGTCACCCTA GACCCCACGC GGGACCAGTG CTTTGGGGAC 
CGCTTCAGCC GCCTATTGCT GGATGAGTTC CTGGGCTATG ATGACATCCT CATGTCCAGT 
GTAAAGGGTC TGGCAGAGAA CGAGGAGAAC AAAGGCTTCT TGAGGAATGT GGTCTCTGGG 
GAGCACTACC GCTTTGTCAG CATGTGGATG GCGCGCACAT CCTACCTGGC GGCCTTTGTC 
ATCATGGTCA TCTTTACCCT CAGCGTGTCC ATGCTGTTGC GATACTCGCA CCACCAGATC 
TTCGTCTTCA TCGTGGACCT GCTGCAGATG CTGGAGATGA ACATGGCCAT CGCCTTCCCC 
GCAGCGCCCT TGCTGACCGT CATCCTGGCT CTCGTCGGGA TGGAAGCCAT CATGTCTGAG 
TTCTTCAATG ATACCACCAC GGCCTTCTAC ATCATCCTCA CTGTGTGGCT GGCCGACCAG 
TATGATGCCA TCTGCTGCCA CACCAACACC AGCAAGCGGC ACTGGCTGAG GTTCTTCTAC 
CTCTACCACT TCGCCTTCTA TGCCTACCAC TACCGCTTTA ACGGGCAGTA CAGCAGCCTG 
GCCCTGGTCA CCTCCTGGCT CTTCATCCAG CATTCCATGA TCTACTTCTT CCACCACTAT 
GAGTTGCCCG CCATCCTGCA GCAGATCCGA ATCCAGGAGA TGCTGCTTCA GACGCCACCG 
CTGGGCCCCG GGACCCCCAC GGCGCTGCCT GACGACCTCA ACAACAACTC TGGCTCCCCT 
GCCACTCCGG ATCCCAGCCC TCCCCTCGCG CTGGGCCCCA GCTCCAGCCC CGCGCCCACT 
GGCGGGGCAT CTGGGCCTGG CTCACTGGGC GCTGGGGCCT CAGTATCCGG CAGTGACCTA 
GGTTGGGTGG CCGAGACCGC CGCCATCATC TCTGACGCAT CCTTCCTGTC GGGGCTGAGC 
GCCTCTCTCC TGGAGCGGCG GCCAACAGCC CCTAGTACCC CGGACAGCTC ACGACCTGAC 
CCTGGGGTCC CTCTGGAGGA CGCACCCGCC CCTGCCGGGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003949.2
CDS	200..1924
Translation	MSEHAAAPGPGPNGGGGGGAAPVRGPRGPNLNPNPLINVRDRLFHALFFKMAVTYSRLFPPAFRRLFEFFVLLKALFVLFVLAYIHIVFSRSPINCLEHVRDRWPREGVLRVEVRHNSSRAPVILQFCDGGLGGLELEPGGLELEEEELTVEMFTNSSIKFELDIEPKVFKPQSGADALNDSQDFPFPETPAKVWPQDEYIVEYSLEYGFLRLSQATRQRLSIPVMVVTLDPTRDQCFGDRFSRLLLDEFLGYDDILMSSVKGLAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIVDLLQMLEMNMAIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILTVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRIQEMLLQTPPLGPGTPTALPDDLNNNSGSPATPDPSPPLALGPSSSPAPTGGASGPGSLGAGASVSGSDLGWVAETAAIISDASFLSGLSASLLERRPTAPSTPDSSRPDPGVPLEDAPAPAGS

Target ORF information:

RefSeq Version	NM_001003949.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protein 259 (Tmem259), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003949.3

ORF Insert Sequence:

ATGTCGGAGC ACGCGGCAGC CCCAGGGCCC GGGCCCAACG GCGGCGGGGG TGGTGGCGCG 
GCGCCCGTGC GCGGCCCTCG CGGCCCCAAT CTCAACCCCA ACCCGCTCAT CAACGTGCGC 
GACCGGCTCT TCCACGCTCT CTTCTTCAAG ATGGCCGTCA CCTACTCACG CCTCTTCCCA 
CCTGCTTTCC GCCGCCTCTT TGAATTCTTC GTTCTGCTCA AGGCCCTGTT TGTGCTTTTC 
GTCCTCGCCT ACATACACAT CGTCTTCTCC CGGTCCCCCA TCAACTGCTT AGAGCATGTT 
CGAGATCGAT GGCCGCGGGA GGGTGTCCTG CGGGTGGAGG TGCGCCACAA CTCGAGCCGG 
GCACCGGTGA TCCTGCAGTT CTGTGATGGG GGCCTCGGTG GCCTGGAGCT GGAACCCGGG 
GGCCTGGAGC TGGAGGAGGA GGAGCTCACA GTGGAGATGT TCACCAACAG CTCCATCAAG 
TTTGAGCTGG ACATTGAGCC CAAGGTGTTC AAGCCACAGA GCGGTGCAGA TGCCCTGAAC 
GACAGCCAGG ACTTCCCTTT TCCTGAGACG CCAGCAAAAG TGTGGCCACA GGATGAGTAC 
ATTGTGGAGT ACTCACTGGA ATATGGCTTC CTGCGGCTAT CCCAAGCCAC ACGCCAGCGT 
CTGAGCATTC CTGTCATGGT GGTCACCCTA GACCCCACGC GGGACCAGTG CTTTGGGGAC 
CGCTTCAGCC GCCTATTGCT GGATGAGTTC CTGGGCTATG ATGACATCCT CATGTCCAGT 
GTAAAGGGTC TGGCAGAGAA CGAGGAGAAC AAAGGCTTCT TGAGGAATGT GGTCTCTGGG 
GAGCACTACC GCTTTGTCAG CATGTGGATG GCGCGCACAT CCTACCTGGC GGCCTTTGTC 
ATCATGGTCA TCTTTACCCT CAGCGTGTCC ATGCTGTTGC GATACTCGCA CCACCAGATC 
TTCGTCTTCA TCGTGGACCT GCTGCAGATG CTGGAGATGA ACATGGCCAT CGCCTTCCCC 
GCAGCGCCCT TGCTGACCGT CATCCTGGCT CTCGTCGGGA TGGAAGCCAT CATGTCTGAG 
TTCTTCAATG ATACCACCAC GGCCTTCTAC ATCATCCTCA CTGTGTGGCT GGCCGACCAG 
TATGATGCCA TCTGCTGCCA CACCAACACC AGCAAGCGGC ACTGGCTGAG GTTCTTCTAC 
CTCTACCACT TCGCCTTCTA TGCCTACCAC TACCGCTTTA ACGGGCAGTA CAGCAGCCTG 
GCCCTGGTCA CCTCCTGGCT CTTCATCCAG CATTCCATGA TCTACTTCTT CCACCACTAT 
GAGTTGCCCG CCATCCTGCA GCAGATCCGA ATCCAGGAGA TGCTGCTTCA GACGCCACCG 
CTGGGCCCCG GGACCCCCAC GGCGCTGCCT GACGACCTCA ACAACAACTC TGGCTCCCCT 
GCCACTCCGG ATCCCAGCCC TCCCCTCGCG CTGGGCCCCA GCTCCAGCCC CGCGCCCACT 
GGCGGGGCAT CTGGGCCTGG CTCACTGGGC GCTGGGGCCT CAGTATCCGG CAGTGACCTA 
GGTTGGGTGG CCGAGACCGC CGCCATCATC TCTGACGCAT CCTTCCTGTC GGGGCTGAGC 
GCCTCTCTCC TGGAGCGGCG GCCAACAGCC CCTAGTACCC CGGACAGCTC ACGACCTGAC 
CCTGGGGTCC CTCTGGAGGA CGCACCCGCC CCTGCCGGGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmem259 ( NM_001359632.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346561.1 Mus musculus transmembrane protein 259 (Tmem259), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu44388
Clone ID Related Accession (Same CDS sequence)	XM_006513490.1 , NM_001359632.1
Accession Version	NM_001359632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	membralin isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC151846.3, BQ572278.1, CX205815.1, BQ748980.1, BQ571954.1, CO044817.1 and BE947335.1. On Jan 13, 2018 this sequence version replaced XM_006513490.1. ##Evidence-Data-START## Transcript exon combination :: AY096037.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAGC ACGCGGCAGC CCCAGGGCCC GGGCCCAACG GCGGCGGGGG TGGTGGCGCG 
GCGCCCGTGC GCGGCCCTCG CGGCCCCAAT CTCAACCCCA ACCCGCTCAT CAACGTGCGC 
GACCGGCTCT TCCACGCTCT CTTCTTCAAG ATGGCCGTCA CCTACTCACG CCTCTTCCCA 
CCTGCTTTCC GCCGCCTCTT TGAATTCTTC GTTCTGCTCA AGGCCCTGTT TGTGCTTTTC 
GTCCTCGCCT ACATACACAT CGTCTTCTCC CGGTCCCCCA TCAACTGCTT AGAGCATGTT 
CGAGATCGAT GGCCGCGGGA GGGTGTCCTG CGGGTGGAGG TGCGCCACAA CTCGAGCCGG 
GCACCGGTGA TCCTGCAGTT CTGTGATGGG GGCCTCGGTG GCCTGGAGCT GGAACCCGGG 
GGCCTGGAGC TGGAGGAGGA GGAGCTCACA GTGGAGATGT TCACCAACAG CTCCATCAAG 
TTTGAGCTGG ACATTGAGCC CAAGGTGTTC AAGCCACAGA GCGGTGCAGA TGCCCTGAAC 
GACAGCCAGG ACTTCCCTTT TCCTGAGACG CCAGCAAAAG TGTGGCCACA GGATGAGTAC 
ATTGTGGAGT ACTCACTGGA ATATGGCTTC CTGCGGCTAT CCCAAGCCAC ACGCCAGCGT 
CTGAGCATTC CTGTCATGGT GGTCACCCTA GACCCCACGC GGGACCAGTG CTTTGGGGAC 
CGCTTCAGCC GCCTATTGCT GGATGAGTTC CTGGGCTATG ATGACATCCT CATGTCCAGT 
GTAAAGGGTC TGGCAGAGAA CGAGGAGAAC AAAGGCTTCT TGAGGAATGT GGTCTCTGGG 
GAGCACTACC GCTTTGTCAG CATGTGGATG GCGCGCACAT CCTACCTGGC GGCCTTTGTC 
ATCATGGTCA TCTTTACCCT CAGCGTGTCC ATGCTGTTGC GATACTCGCA CCACCAGATC 
TTCGTCTTCA TCGGTGAGTC CCAGCCGGCC GGGGGTGGGG TGGGCTCTCC CCAGGCCCTC 
ATGGGCGGCC GCCCTGTGCC CGCAGTGGAC CTGCTGCAGA TGCTGGAGAT GAACATGGCC 
ATCGCCTTCC CCGCAGCGCC CTTGCTGACC GTCATCCTGG CTCTCGTCGG GATGGAAGCC 
ATCATGTCTG AGTTCTTCAA TGATACCACC ACGGCCTTCT ACATCATCCT CACTGTGTGG 
CTGGCCGACC AGTATGATGC CATCTGCTGC CACACCAACA CCAGCAAGCG GCACTGGCTG 
AGGTTCTTCT ACCTCTACCA CTTCGCCTTC TATGCCTACC ACTACCGCTT TAACGGGCAG 
TACAGCAGCC TGGCCCTGGT CACCTCCTGG CTCTTCATCC AGCATTCCAT GATCTACTTC 
TTCCACCACT ATGAGTTGCC CGCCATCCTG CAGCAGATCC GAATCCAGGA GATGCTGCTT 
CAGACGCCAC CGCTGGGCCC CGGGACCCCC ACGGCGCTGC CTGACGACCT CAACAACAAC 
TCTGGCTCCC CTGCCACTCC GGATCCCAGC CCTCCCCTCG CGCTGGGCCC CAGCTCCAGC 
CCCGCGCCCA CTGGCGGGGC ATCTGGGCCT GGCTCACTGG GCGCTGGGGC CTCAGTATCC 
GGCAGTGACC TAGGTTGGGT GGCCGAGACC GCCGCCATCA TCTCTGACGC ATCCTTCCTG 
TCGGGGCTGA GCGCCTCTCT CCTGGAGCGG CGGCCAACAG CCCCTAGTAC CCCGGACAGC 
TCACGACCTG ACCCTGGGGT CCCTCTGGAG GACGCACCCG CCCCTGCCGG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346561.1
CDS	130..1926
Translation	MSEHAAAPGPGPNGGGGGGAAPVRGPRGPNLNPNPLINVRDRLFHALFFKMAVTYSRLFPPAFRRLFEFFVLLKALFVLFVLAYIHIVFSRSPINCLEHVRDRWPREGVLRVEVRHNSSRAPVILQFCDGGLGGLELEPGGLELEEEELTVEMFTNSSIKFELDIEPKVFKPQSGADALNDSQDFPFPETPAKVWPQDEYIVEYSLEYGFLRLSQATRQRLSIPVMVVTLDPTRDQCFGDRFSRLLLDEFLGYDDILMSSVKGLAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIGESQPAGGGVGSPQALMGGRPVPAVDLLQMLEMNMAIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILTVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRIQEMLLQTPPLGPGTPTALPDDLNNNSGSPATPDPSPPLALGPSSSPAPTGGASGPGSLGAGASVSGSDLGWVAETAAIISDASFLSGLSASLLERRPTAPSTPDSSRPDPGVPLEDAPAPAGS

Target ORF information:

RefSeq Version	NM_001359632.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protein 259 (Tmem259), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359632.1

ORF Insert Sequence:

ATGTCGGAGC ACGCGGCAGC CCCAGGGCCC GGGCCCAACG GCGGCGGGGG TGGTGGCGCG 
GCGCCCGTGC GCGGCCCTCG CGGCCCCAAT CTCAACCCCA ACCCGCTCAT CAACGTGCGC 
GACCGGCTCT TCCACGCTCT CTTCTTCAAG ATGGCCGTCA CCTACTCACG CCTCTTCCCA 
CCTGCTTTCC GCCGCCTCTT TGAATTCTTC GTTCTGCTCA AGGCCCTGTT TGTGCTTTTC 
GTCCTCGCCT ACATACACAT CGTCTTCTCC CGGTCCCCCA TCAACTGCTT AGAGCATGTT 
CGAGATCGAT GGCCGCGGGA GGGTGTCCTG CGGGTGGAGG TGCGCCACAA CTCGAGCCGG 
GCACCGGTGA TCCTGCAGTT CTGTGATGGG GGCCTCGGTG GCCTGGAGCT GGAACCCGGG 
GGCCTGGAGC TGGAGGAGGA GGAGCTCACA GTGGAGATGT TCACCAACAG CTCCATCAAG 
TTTGAGCTGG ACATTGAGCC CAAGGTGTTC AAGCCACAGA GCGGTGCAGA TGCCCTGAAC 
GACAGCCAGG ACTTCCCTTT TCCTGAGACG CCAGCAAAAG TGTGGCCACA GGATGAGTAC 
ATTGTGGAGT ACTCACTGGA ATATGGCTTC CTGCGGCTAT CCCAAGCCAC ACGCCAGCGT 
CTGAGCATTC CTGTCATGGT GGTCACCCTA GACCCCACGC GGGACCAGTG CTTTGGGGAC 
CGCTTCAGCC GCCTATTGCT GGATGAGTTC CTGGGCTATG ATGACATCCT CATGTCCAGT 
GTAAAGGGTC TGGCAGAGAA CGAGGAGAAC AAAGGCTTCT TGAGGAATGT GGTCTCTGGG 
GAGCACTACC GCTTTGTCAG CATGTGGATG GCGCGCACAT CCTACCTGGC GGCCTTTGTC 
ATCATGGTCA TCTTTACCCT CAGCGTGTCC ATGCTGTTGC GATACTCGCA CCACCAGATC 
TTCGTCTTCA TCGGTGAGTC CCAGCCGGCC GGGGGTGGGG TGGGCTCTCC CCAGGCCCTC 
ATGGGCGGCC GCCCTGTGCC CGCAGTGGAC CTGCTGCAGA TGCTGGAGAT GAACATGGCC 
ATCGCCTTCC CCGCAGCGCC CTTGCTGACC GTCATCCTGG CTCTCGTCGG GATGGAAGCC 
ATCATGTCTG AGTTCTTCAA TGATACCACC ACGGCCTTCT ACATCATCCT CACTGTGTGG 
CTGGCCGACC AGTATGATGC CATCTGCTGC CACACCAACA CCAGCAAGCG GCACTGGCTG 
AGGTTCTTCT ACCTCTACCA CTTCGCCTTC TATGCCTACC ACTACCGCTT TAACGGGCAG 
TACAGCAGCC TGGCCCTGGT CACCTCCTGG CTCTTCATCC AGCATTCCAT GATCTACTTC 
TTCCACCACT ATGAGTTGCC CGCCATCCTG CAGCAGATCC GAATCCAGGA GATGCTGCTT 
CAGACGCCAC CGCTGGGCCC CGGGACCCCC ACGGCGCTGC CTGACGACCT CAACAACAAC 
TCTGGCTCCC CTGCCACTCC GGATCCCAGC CCTCCCCTCG CGCTGGGCCC CAGCTCCAGC 
CCCGCGCCCA CTGGCGGGGC ATCTGGGCCT GGCTCACTGG GCGCTGGGGC CTCAGTATCC 
GGCAGTGACC TAGGTTGGGT GGCCGAGACC GCCGCCATCA TCTCTGACGC ATCCTTCCTG 
TCGGGGCTGA GCGCCTCTCT CCTGGAGCGG CGGCCAACAG CCCCTAGTAC CCCGGACAGC 
TCACGACCTG ACCCTGGGGT CCCTCTGGAG GACGCACCCG CCCCTGCCGG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmem259 ( XM_006513490.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513553.1 Mus musculus transmembrane protein 259 (Tmem259), transcript variant X1, mRNA.

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CloneID	OMu44388
Clone ID Related Accession (Same CDS sequence)	XM_006513490.1 , NM_001359632.1
Accession Version	XM_006513490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	membralin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGC ACGCGGCAGC CCCAGGGCCC GGGCCCAACG GCGGCGGGGG TGGTGGCGCG 
GCGCCCGTGC GCGGCCCTCG CGGCCCCAAT CTCAACCCCA ACCCGCTCAT CAACGTGCGC 
GACCGGCTCT TCCACGCTCT CTTCTTCAAG ATGGCCGTCA CCTACTCACG CCTCTTCCCA 
CCTGCTTTCC GCCGCCTCTT TGAATTCTTC GTTCTGCTCA AGGCCCTGTT TGTGCTTTTC 
GTCCTCGCCT ACATACACAT CGTCTTCTCC CGGTCCCCCA TCAACTGCTT AGAGCATGTT 
CGAGATCGAT GGCCGCGGGA GGGTGTCCTG CGGGTGGAGG TGCGCCACAA CTCGAGCCGG 
GCACCGGTGA TCCTGCAGTT CTGTGATGGG GGCCTCGGTG GCCTGGAGCT GGAACCCGGG 
GGCCTGGAGC TGGAGGAGGA GGAGCTCACA GTGGAGATGT TCACCAACAG CTCCATCAAG 
TTTGAGCTGG ACATTGAGCC CAAGGTGTTC AAGCCACAGA GCGGTGCAGA TGCCCTGAAC 
GACAGCCAGG ACTTCCCTTT TCCTGAGACG CCAGCAAAAG TGTGGCCACA GGATGAGTAC 
ATTGTGGAGT ACTCACTGGA ATATGGCTTC CTGCGGCTAT CCCAAGCCAC ACGCCAGCGT 
CTGAGCATTC CTGTCATGGT GGTCACCCTA GACCCCACGC GGGACCAGTG CTTTGGGGAC 
CGCTTCAGCC GCCTATTGCT GGATGAGTTC CTGGGCTATG ATGACATCCT CATGTCCAGT 
GTAAAGGGTC TGGCAGAGAA CGAGGAGAAC AAAGGCTTCT TGAGGAATGT GGTCTCTGGG 
GAGCACTACC GCTTTGTCAG CATGTGGATG GCGCGCACAT CCTACCTGGC GGCCTTTGTC 
ATCATGGTCA TCTTTACCCT CAGCGTGTCC ATGCTGTTGC GATACTCGCA CCACCAGATC 
TTCGTCTTCA TCGGTGAGTC CCAGCCGGCC GGGGGTGGGG TGGGCTCTCC CCAGGCCCTC 
ATGGGCGGCC GCCCTGTGCC CGCAGTGGAC CTGCTGCAGA TGCTGGAGAT GAACATGGCC 
ATCGCCTTCC CCGCAGCGCC CTTGCTGACC GTCATCCTGG CTCTCGTCGG GATGGAAGCC 
ATCATGTCTG AGTTCTTCAA TGATACCACC ACGGCCTTCT ACATCATCCT CACTGTGTGG 
CTGGCCGACC AGTATGATGC CATCTGCTGC CACACCAACA CCAGCAAGCG GCACTGGCTG 
AGGTTCTTCT ACCTCTACCA CTTCGCCTTC TATGCCTACC ACTACCGCTT TAACGGGCAG 
TACAGCAGCC TGGCCCTGGT CACCTCCTGG CTCTTCATCC AGCATTCCAT GATCTACTTC 
TTCCACCACT ATGAGTTGCC CGCCATCCTG CAGCAGATCC GAATCCAGGA GATGCTGCTT 
CAGACGCCAC CGCTGGGCCC CGGGACCCCC ACGGCGCTGC CTGACGACCT CAACAACAAC 
TCTGGCTCCC CTGCCACTCC GGATCCCAGC CCTCCCCTCG CGCTGGGCCC CAGCTCCAGC 
CCCGCGCCCA CTGGCGGGGC ATCTGGGCCT GGCTCACTGG GCGCTGGGGC CTCAGTATCC 
GGCAGTGACC TAGGTTGGGT GGCCGAGACC GCCGCCATCA TCTCTGACGC ATCCTTCCTG 
TCGGGGCTGA GCGCCTCTCT CCTGGAGCGG CGGCCAACAG CCCCTAGTAC CCCGGACAGC 
TCACGACCTG ACCCTGGGGT CCCTCTGGAG GACGCACCCG CCCCTGCCGG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513553.1
CDS	195..1991
Translation	MSEHAAAPGPGPNGGGGGGAAPVRGPRGPNLNPNPLINVRDRLFHALFFKMAVTYSRLFPPAFRRLFEFFVLLKALFVLFVLAYIHIVFSRSPINCLEHVRDRWPREGVLRVEVRHNSSRAPVILQFCDGGLGGLELEPGGLELEEEELTVEMFTNSSIKFELDIEPKVFKPQSGADALNDSQDFPFPETPAKVWPQDEYIVEYSLEYGFLRLSQATRQRLSIPVMVVTLDPTRDQCFGDRFSRLLLDEFLGYDDILMSSVKGLAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIGESQPAGGGVGSPQALMGGRPVPAVDLLQMLEMNMAIAFPAAPLLTVILALVGMEAIMSEFFNDTTTAFYIILTVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRIQEMLLQTPPLGPGTPTALPDDLNNNSGSPATPDPSPPLALGPSSSPAPTGGASGPGSLGAGASVSGSDLGWVAETAAIISDASFLSGLSASLLERRPTAPSTPDSSRPDPGVPLEDAPAPAGS

Target ORF information:

RefSeq Version	XM_006513490.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protein 259 (Tmem259), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513490.1

ORF Insert Sequence:

ATGTCGGAGC ACGCGGCAGC CCCAGGGCCC GGGCCCAACG GCGGCGGGGG TGGTGGCGCG 
GCGCCCGTGC GCGGCCCTCG CGGCCCCAAT CTCAACCCCA ACCCGCTCAT CAACGTGCGC 
GACCGGCTCT TCCACGCTCT CTTCTTCAAG ATGGCCGTCA CCTACTCACG CCTCTTCCCA 
CCTGCTTTCC GCCGCCTCTT TGAATTCTTC GTTCTGCTCA AGGCCCTGTT TGTGCTTTTC 
GTCCTCGCCT ACATACACAT CGTCTTCTCC CGGTCCCCCA TCAACTGCTT AGAGCATGTT 
CGAGATCGAT GGCCGCGGGA GGGTGTCCTG CGGGTGGAGG TGCGCCACAA CTCGAGCCGG 
GCACCGGTGA TCCTGCAGTT CTGTGATGGG GGCCTCGGTG GCCTGGAGCT GGAACCCGGG 
GGCCTGGAGC TGGAGGAGGA GGAGCTCACA GTGGAGATGT TCACCAACAG CTCCATCAAG 
TTTGAGCTGG ACATTGAGCC CAAGGTGTTC AAGCCACAGA GCGGTGCAGA TGCCCTGAAC 
GACAGCCAGG ACTTCCCTTT TCCTGAGACG CCAGCAAAAG TGTGGCCACA GGATGAGTAC 
ATTGTGGAGT ACTCACTGGA ATATGGCTTC CTGCGGCTAT CCCAAGCCAC ACGCCAGCGT 
CTGAGCATTC CTGTCATGGT GGTCACCCTA GACCCCACGC GGGACCAGTG CTTTGGGGAC 
CGCTTCAGCC GCCTATTGCT GGATGAGTTC CTGGGCTATG ATGACATCCT CATGTCCAGT 
GTAAAGGGTC TGGCAGAGAA CGAGGAGAAC AAAGGCTTCT TGAGGAATGT GGTCTCTGGG 
GAGCACTACC GCTTTGTCAG CATGTGGATG GCGCGCACAT CCTACCTGGC GGCCTTTGTC 
ATCATGGTCA TCTTTACCCT CAGCGTGTCC ATGCTGTTGC GATACTCGCA CCACCAGATC 
TTCGTCTTCA TCGGTGAGTC CCAGCCGGCC GGGGGTGGGG TGGGCTCTCC CCAGGCCCTC 
ATGGGCGGCC GCCCTGTGCC CGCAGTGGAC CTGCTGCAGA TGCTGGAGAT GAACATGGCC 
ATCGCCTTCC CCGCAGCGCC CTTGCTGACC GTCATCCTGG CTCTCGTCGG GATGGAAGCC 
ATCATGTCTG AGTTCTTCAA TGATACCACC ACGGCCTTCT ACATCATCCT CACTGTGTGG 
CTGGCCGACC AGTATGATGC CATCTGCTGC CACACCAACA CCAGCAAGCG GCACTGGCTG 
AGGTTCTTCT ACCTCTACCA CTTCGCCTTC TATGCCTACC ACTACCGCTT TAACGGGCAG 
TACAGCAGCC TGGCCCTGGT CACCTCCTGG CTCTTCATCC AGCATTCCAT GATCTACTTC 
TTCCACCACT ATGAGTTGCC CGCCATCCTG CAGCAGATCC GAATCCAGGA GATGCTGCTT 
CAGACGCCAC CGCTGGGCCC CGGGACCCCC ACGGCGCTGC CTGACGACCT CAACAACAAC 
TCTGGCTCCC CTGCCACTCC GGATCCCAGC CCTCCCCTCG CGCTGGGCCC CAGCTCCAGC 
CCCGCGCCCA CTGGCGGGGC ATCTGGGCCT GGCTCACTGG GCGCTGGGGC CTCAGTATCC 
GGCAGTGACC TAGGTTGGGT GGCCGAGACC GCCGCCATCA TCTCTGACGC ATCCTTCCTG 
TCGGGGCTGA GCGCCTCTCT CCTGGAGCGG CGGCCAACAG CCCCTAGTAC CCCGGACAGC 
TCACGACCTG ACCCTGGGGT CCCTCTGGAG GACGCACCCG CCCCTGCCGG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmem259 ( XM_006513491.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513554.1 Mus musculus transmembrane protein 259 (Tmem259), transcript variant X1, mRNA.

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CloneID	OMu44389
Clone ID Related Accession (Same CDS sequence)	XM_006513491.1
Accession Version	XM_006513491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	membralin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGC ACGCGGCAGC CCCAGGGCCC GGGCCCAACG GCGGCGGGGG TGGTGGCGCG 
GCGCCCGTGC GCGGCCCTCG CGGCCCCAAT CTCAACCCCA ACCCGCTCAT CAACGTGCGC 
GACCGGCTCT TCCACGCTCT CTTCTTCAAG ATGGCCGTCA CCTACTCACG CCTCTTCCCA 
CCTGCTTTCC GCCGCCTCTT TGAATTCTTC GTTCTGCTCA AGGCCCTGTT TGTGCTTTTC 
GTCCTCGCCT ACATACACAT CGTCTTCTCC CGGTCCCCCA TCAACTGCTT AGAGCATGTT 
CGAGATCGAT GGCCGCGGGA GGGTGTCCTG CGGGTGGAGG TGCGCCACAA CTCGAGCCGG 
GCACCGGTGA TCCTGCAGTT CTGTGATGGG GGCCTCGGTG GCCTGGAGCT GGAACCCGGG 
GGCCTGGAGC TGGAGGAGGA GGAGCTCACA GTGGAGATGT TCACCAACAG CTCCATCAAG 
TTTGAGCTGG ACATTGAGCC CAAGGTGTTC AAGCCACAGA GCGGTGCAGA TGCCCTGAAC 
GACAGCCAGG ACTTCCCTTT TCCTGAGACG CCAGCAAAAG TGTGGCCACA GGATGAGTAC 
ATTGTGGAGT ACTCACTGGA ATATGGCTTC CTGCGGCTAT CCCAAGCCAC ACGCCAGCGT 
CTGAGCATTC CTGTCATGGT GGTCACCCTA GACCCCACGC GGGACCAGTG CTTTGGGGAC 
CGCTTCAGCC GCCTATTGCT GGATGAGTTC CTGGGCTATG ATGACATCCT CATGTCCAGT 
GTAAAGGGTC TGGCAGAGAA CGAGGAGAAC AAAGGCTTCT TGAGGAATGT GGTCTCTGGG 
GAGCACTACC GCTTTGTCAG CATGTGGATG GCGCGCACAT CCTACCTGGC GGCCTTTGTC 
ATCATGGTCA TCTTTACCCT CAGCGTGTCC ATGCTGTTGC GATACTCGCA CCACCAGATC 
TTCGTCTTCA TCGCGCCCTT GCTGACCGTC ATCCTGGCTC TCGTCGGGAT GGAAGCCATC 
ATGTCTGAGT TCTTCAATGA TACCACCACG GCCTTCTACA TCATCCTCAC TGTGTGGCTG 
GCCGACCAGT ATGATGCCAT CTGCTGCCAC ACCAACACCA GCAAGCGGCA CTGGCTGAGG 
TTCTTCTACC TCTACCACTT CGCCTTCTAT GCCTACCACT ACCGCTTTAA CGGGCAGTAC 
AGCAGCCTGG CCCTGGTCAC CTCCTGGCTC TTCATCCAGC ATTCCATGAT CTACTTCTTC 
CACCACTATG AGTTGCCCGC CATCCTGCAG CAGATCCGAA TCCAGGAGAT GCTGCTTCAG 
ACGCCACCGC TGGGCCCCGG GACCCCCACG GCGCTGCCTG ACGACCTCAA CAACAACTCT 
GGCTCCCCTG CCACTCCGGA TCCCAGCCCT CCCCTCGCGC TGGGCCCCAG CTCCAGCCCC 
GCGCCCACTG GCGGGGCATC TGGGCCTGGC TCACTGGGCG CTGGGGCCTC AGTATCCGGC 
AGTGACCTAG GTTGGGTGGC CGAGACCGCC GCCATCATCT CTGACGCATC CTTCCTGTCG 
GGGCTGAGCG CCTCTCTCCT GGAGCGGCGG CCAACAGCCC CTAGTACCCC GGACAGCTCA 
CGACCTGACC CTGGGGTCCC TCTGGAGGAC GCACCCGCCC CTGCCGGGTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513554.1
CDS	195..1868
Translation	MSEHAAAPGPGPNGGGGGGAAPVRGPRGPNLNPNPLINVRDRLFHALFFKMAVTYSRLFPPAFRRLFEFFVLLKALFVLFVLAYIHIVFSRSPINCLEHVRDRWPREGVLRVEVRHNSSRAPVILQFCDGGLGGLELEPGGLELEEEELTVEMFTNSSIKFELDIEPKVFKPQSGADALNDSQDFPFPETPAKVWPQDEYIVEYSLEYGFLRLSQATRQRLSIPVMVVTLDPTRDQCFGDRFSRLLLDEFLGYDDILMSSVKGLAENEENKGFLRNVVSGEHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIAPLLTVILALVGMEAIMSEFFNDTTTAFYIILTVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSLALVTSWLFIQHSMIYFFHHYELPAILQQIRIQEMLLQTPPLGPGTPTALPDDLNNNSGSPATPDPSPPLALGPSSSPAPTGGASGPGSLGAGASVSGSDLGWVAETAAIISDASFLSGLSASLLERRPTAPSTPDSSRPDPGVPLEDAPAPAGS

Target ORF information:

RefSeq Version	XM_006513491.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus transmembrane protein 259 (Tmem259), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513491.1

ORF Insert Sequence:

ATGTCGGAGC ACGCGGCAGC CCCAGGGCCC GGGCCCAACG GCGGCGGGGG TGGTGGCGCG 
GCGCCCGTGC GCGGCCCTCG CGGCCCCAAT CTCAACCCCA ACCCGCTCAT CAACGTGCGC 
GACCGGCTCT TCCACGCTCT CTTCTTCAAG ATGGCCGTCA CCTACTCACG CCTCTTCCCA 
CCTGCTTTCC GCCGCCTCTT TGAATTCTTC GTTCTGCTCA AGGCCCTGTT TGTGCTTTTC 
GTCCTCGCCT ACATACACAT CGTCTTCTCC CGGTCCCCCA TCAACTGCTT AGAGCATGTT 
CGAGATCGAT GGCCGCGGGA GGGTGTCCTG CGGGTGGAGG TGCGCCACAA CTCGAGCCGG 
GCACCGGTGA TCCTGCAGTT CTGTGATGGG GGCCTCGGTG GCCTGGAGCT GGAACCCGGG 
GGCCTGGAGC TGGAGGAGGA GGAGCTCACA GTGGAGATGT TCACCAACAG CTCCATCAAG 
TTTGAGCTGG ACATTGAGCC CAAGGTGTTC AAGCCACAGA GCGGTGCAGA TGCCCTGAAC 
GACAGCCAGG ACTTCCCTTT TCCTGAGACG CCAGCAAAAG TGTGGCCACA GGATGAGTAC 
ATTGTGGAGT ACTCACTGGA ATATGGCTTC CTGCGGCTAT CCCAAGCCAC ACGCCAGCGT 
CTGAGCATTC CTGTCATGGT GGTCACCCTA GACCCCACGC GGGACCAGTG CTTTGGGGAC 
CGCTTCAGCC GCCTATTGCT GGATGAGTTC CTGGGCTATG ATGACATCCT CATGTCCAGT 
GTAAAGGGTC TGGCAGAGAA CGAGGAGAAC AAAGGCTTCT TGAGGAATGT GGTCTCTGGG 
GAGCACTACC GCTTTGTCAG CATGTGGATG GCGCGCACAT CCTACCTGGC GGCCTTTGTC 
ATCATGGTCA TCTTTACCCT CAGCGTGTCC ATGCTGTTGC GATACTCGCA CCACCAGATC 
TTCGTCTTCA TCGCGCCCTT GCTGACCGTC ATCCTGGCTC TCGTCGGGAT GGAAGCCATC 
ATGTCTGAGT TCTTCAATGA TACCACCACG GCCTTCTACA TCATCCTCAC TGTGTGGCTG 
GCCGACCAGT ATGATGCCAT CTGCTGCCAC ACCAACACCA GCAAGCGGCA CTGGCTGAGG 
TTCTTCTACC TCTACCACTT CGCCTTCTAT GCCTACCACT ACCGCTTTAA CGGGCAGTAC 
AGCAGCCTGG CCCTGGTCAC CTCCTGGCTC TTCATCCAGC ATTCCATGAT CTACTTCTTC 
CACCACTATG AGTTGCCCGC CATCCTGCAG CAGATCCGAA TCCAGGAGAT GCTGCTTCAG 
ACGCCACCGC TGGGCCCCGG GACCCCCACG GCGCTGCCTG ACGACCTCAA CAACAACTCT 
GGCTCCCCTG CCACTCCGGA TCCCAGCCCT CCCCTCGCGC TGGGCCCCAG CTCCAGCCCC 
GCGCCCACTG GCGGGGCATC TGGGCCTGGC TCACTGGGCG CTGGGGCCTC AGTATCCGGC 
AGTGACCTAG GTTGGGTGGC CGAGACCGCC GCCATCATCT CTGACGCATC CTTCCTGTCG 
GGGCTGAGCG CCTCTCTCCT GGAGCGGCGG CCAACAGCCC CTAGTACCCC GGACAGCTCA 
CGACCTGACC CTGGGGTCCC TCTGGAGGAC GCACCCGCCC CTGCCGGGTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ER-associated degradation regulates Alzheimer's amyloid pathology and memory function by modulating ?-secretase activity.
Nature communications8(1)1472(2017 Nov)
Zhu B,Jiang L,Huang T,Zhao Y,Liu T,Zhong Y,Li X,Campos A,Pomeroy K,Masliah E,Zhang D,Xu H

The critical role of membralin in postnatal motor neuron survival and disease.
eLife4(2015 May)
Yang B,Qu M,Wang R,Chatterton JE,Liu XB,Zhu B,Narisawa S,Millan JL,Nakanishi N,Swoboda K,Lipton SA,Zhang D

Characterization and expression of the gene encoding membralin, an evolutionary conserved protein expressed in the central nervous system.
Brain research. Gene expression patterns1(3-4)205-12(2002 Oct)
Andersson O,von Euler G

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Tmem259 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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