Hk3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hk3
Entrez Gene ID	212032
Full Name	hexokinase 3
Synonyms	HK III,HK-III
General protein information	Preferred Names hexokinase 3 Names hexokinase-3 hexokinase type III
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001033245.4	NP_001028417.1	hexokinase-3 isoform 1
XM_006517193.3	XP_006517256.1	hexokinase-3 isoform X1
NM_001206392.1	NP_001193321.1	hexokinase-3 isoform 3
NM_001206391.1	NP_001193320.1	hexokinase-3 isoform 2
NM_001206390.1	NP_001193319.1	hexokinase-3 isoform 1
XM_006517193.4	XP_006517256.1	hexokinase-3 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HK3	XP_518124.2
	HK3	XP_001086179.1
Mus musculus (house mouse)	Hk3	NP_001028417.1
Gallus gallus (chicken)	HK3	XP_001231329.3
Homo sapiens (human)	HK3	NP_002106.2
Canis lupus familiaris (dog)	HK3	XP_546212.4
Rattus norvegicus (Norway rat)	Hk3	NP_071515.1

Gene Ontology
Bibliography

Related articles in PubMed

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Bulusu V, Prior N, Snaebjornsson MT, Kuehne A, Sonnen KF, Kress J, Stein F, Schultz C, Sauer U, Aulehla A
Developmental cell40(4)331-341.e4(2017 02)

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Bowman CE, Zhao L, Hartung T, Wolfgang MJ
Molecular and cellular biology36(15)2089-104(2016 Aug)

Chronic Activation of ?2 AMPK Induces Obesity and Reduces ? Cell Function.
Yavari A, Stocker CJ, Ghaffari S, Wargent ET, Steeples V, Czibik G, Pinter K, Bellahcene M, Woods A, Mart?nez de Morentin PB, Cansell C, Lam BY, Chuster A, Petkevicius K, Nguyen-Tu MS, Martinez-Sanchez A, Pullen TJ, Oliver PL, Stockenhuber A, Nguyen C, Lazdam M, O'Dowd JF, Harikumar P, T?th M, Beall C, Kyriakou T, Parnis J, Sarma D, Katritsis G, Wortmann DD, Harper AR, Brown LA, Willows R, Gandra S, Poncio V, de Oliveira Figueiredo MJ, Qi NR, Peirson SN, McCrimmon RJ, Gereben B, Tretter L, Fekete C, Redwood C, Yeo GS, Heisler LK, Rutter GA, Smith MA, Withers DJ, Carling D, Sternick EB, Arch JR, Cawthorne MA, Watkins H, Ashrafian H
Cell metabolism23(5)821-36(2016 May)

Direct neuronal glucose uptake heralds activity-dependent increases in cerebral metabolism.
Lundgaard I, Li B, Xie L, Kang H, Sanggaard S, Haswell JD, Sun W, Goldman S, Blekot S, Nielsen M, Takano T, Deane R, Nedergaard M
Nature communications66807(2015 Apr)

Lactate downregulates the glycolytic enzymes hexokinase and phosphofructokinase in diverse tissues from mice.
Leite TC, Coelho RG, Da Silva D, Coelho WS, Marinho-Carvalho MM, Sola-Penna M
FEBS letters585(1)92-8(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hexokinase expression is highly enriched in neurons compared to astrocytes.
Title: Direct neuronal glucose uptake heralds activity-dependent increases in cerebral metabolism.

The following Hk3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hk3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu12866	NM_001206392.1 Latest version!	Mus musculus hexokinase 3 (Hk3), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu12903	NM_001206391.1 Latest version!	Mus musculus hexokinase 3 (Hk3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu12949	NM_001206390.1 Latest version!	Mus musculus hexokinase 3 (Hk3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu12949	NM_001033245.4 Latest version!	Mus musculus hexokinase 3 (Hk3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu48717	XM_006517193.4 Latest version!	Mus musculus hexokinase 3 (Hk3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OMu48717	XM_006517193.3	Mus musculus hexokinase 3 (Hk3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hk3 ( NM_001206392.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001193321.1 Mus musculus hexokinase 3 (Hk3), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu12866
Clone ID Related Accession (Same CDS sequence)	NM_001206392.1
Accession Version	NM_001206392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	hexokinase-3 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154610.3. Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK159857.1, AK170813.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGAGCAGG CACTGAAGGG ACAGGACAGT CCCGCTCCTT CTGTCCGGAT GCTGCCCACA 
TACGTGAGGT CCACACCACA CGGCACCGAG CAAGGGGATT TCCTGGTGCT GGAGCTGGGG 
GCCACAGGAG CATCACTACG TGTGTTGTGG GTAACACTGA CAGGCACCAA AGAATGCCGG 
GTGGAGCCCA GGAGCCGGGA GTTTGTGATT CCTCAAGAGG TGATCCTAGG TGCTGGCCAG 
CAGCTCTTTG ACTTTGCTGC CCGATGCCTC TCTGAATTCC TGGATGCATA CCCCGTGGAG 
AATCAGGGTC TGAAGCTTGG GTTCAATTTC TCTTTTCCTT GTCACCAGAC AGGCTTGGAC 
AGGAGCACCC TCATTTCCTG GACAAAAGGT TTTAGGTGCA GCGGCGTGGA AGGCCAGGAT 
GTGGTCCAGT TGCTAAGAGA TGCCATTCAG AGGCAGGGGA CCTACAGGAT TGATGTGGTA 
GCCATGGTGA ATGACACGGT GGGCACCATG ATGGGCTGTG AGCTGGGCAC CAGGCCGTGT 
GAAGTCGGGC TGATTGTAGA CACCGGTACC AACGCGTGCT ACATGGAGGA GGCGCGGCAC 
GTGGCCGCTC TGGATGAGGA CCGCGGCCGC ACCTGTGTCA GCATCGAGTG GGGCTCCTTC 
TATGACGAAG ATGCTCTAGG GCCAGTACTG ACCACCTTCG ACTCTGCCCT GGACCGCGAG 
TCCCTGACTC CTGGTGCTCA AAGGTTTGAG AAGATGATTG GAGGCCTTTA CCTGGGTGAG 
CTGGTGAGGC TGGTTCTGGT CCACTTGACC CAGCACGGGG TCCTCTTTGA TGGCTGCGCC 
TCTCCTGCGT TGCTGAGTCA AGGCTGTATC CTCCTGGACC ACGTGGCTGA GATGGAGGAC 
ACCGCCACTG GGACAGCCCG CGTCCACACC ATCCTGCAGG ACTTGGGTCT GAGCCCTCGG 
GCCTCAGATG CTGAACTGGT GCAATATGTG TGCGTGGCTG TGTGCACACG GGCTGCCCAG 
CTCTGTGCGG CTGCCCTGGC TGCAGTCCTC TCCCGCCTCC AGCACAGCAG GGAGCAGCAG 
ACATTGCAAG TGGCTGTGGC CACTGGAGGG CGAGTGTTTG AACGACACCC CAGGTTCCTC 
CGAATCCTAA AGGAGACGGT AACACTCCTG GCCCCAAACT GTGATGTCTC CTTCATCCCC 
TCTGTGGATG GTGGTGGCCG GGGTGTGGCA ATGGTGACAG CTGTGGCTGC CCGCCTGGCT 
GCTCACAGGC GCATCCTGGA AGAGACCCTG GCACCATTTC AGCTGACCTT GGAGCAGATG 
ACAGTGGTGC AGGCACAAAT GCGGGAAGCC ATGATCAGGG GGCTTCAAGG AGAGGCCTCC 
TCCCTCCGCA TGCTGCCCAC TTACGTCCGA GCGACGCCCG ACGGCAGCGA GCGAGGTGAT 
TTCCTGGCCT TGGACCTAGG GGGCACCAAC TTCCGGGTCC TGTTGGTACG AGTGGCGGAG 
GGCAGTGTGC AGATCATCAA CCAGGTCTAC TCCATTCCTG AGTGTAGAGC CCAGGGCTCT 
GGACAGAAGC TCTTTGACCA TATTGTGGAC TGCATCGTGG ACTTCCAGAA GAGGCAGGGC 
CTTAGTGGAC AGAGCCTACC CCTGGGTTTC ACCTTCTCTT TCCCATGCAA GCAGCTTGGC 
CTGGACCAGG GCATCCTCCT GAATTGGACT AAGGGGTTCA ATGCATCAGG CTGTGAGGGC 
CAGGATGTTG TGTATCTATT ACGGGAAGCC ATTAGGCGCA GACAGGCAGT GGAGCTGAAT 
GTGGTTGCCA TTGTCAATGA CACGGTGGGG ACCATGATGT CCTGTGGCTA TGATGATCCC 
CGTTGTGAGA TGGGCCTCAT TGTCGGAACC GGCACCAACG CCTGTTACAT GGAAGAGCTC 
CGCAATGTGG CAAGTGTGCC TGGGGACTCG GGCCTCATGT GCATCAACAT GGAGTGGGGT 
GCCTTTGGGG ATGATGGCTC ACTGGGCACA CTCAGCACCC GCTTTGACAC CAGTGTGGAC 
CAGGCATCCA TCAATCCAGG CAAACAGAGG TTTGAGAAAA TGATCAGCGG TATGTACTTG 
GGGGAGATCG TCCGCCATAT CCTCCTGCAC TTAACCAATC TCGGAGTTCT CTTCCGAGGC 
CAGAAGACTC AATGCCTTCA GGCCAGGGAC ATCTTCAAGA CTAAGTTCCT CTCTGAGATC 
GAAAGTGATA GCCTGGCCCT GCGTCAGGTC CGAGCCATCC TGGAAGATCT GGGGCTGACT 
CTGACATCTG ATGACGCCTT GATGGTCCTG GAAGTGTGCC AGGCTGTGTC TCGCAGGGCT 
GCCCAACTCT GTGGGGCAGG TGTGGCTGCC GTGGTGGAGA AGATTCGGGA GAACCGGGGC 
CTGCAGGAGC TGACAGTGTC TGTGGGAGTA GATGGGACAC TCTACAAGCT ACATCCTCAC 
TTCTCCAAGC TGGTGTCGGC AACAGTCCGG AAGCTAGCCC CTCAGTGCAC CGTCACCTTT 
TTGCAGTCGG AGGATGGGTC TGGCAAAGGC GCAGCTTTGG TTACTGCTGT TGCTTGTCGC 
CTTACCCAGA TGGCCCACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193321.1
CDS	310..2913
Translation	MEQALKGQDSPAPSVRMLPTYVRSTPHGTEQGDFLVLELGATGASLRVLWVTLTGTKECRVEPRSREFVIPQEVILGAGQQLFDFAARCLSEFLDAYPVENQGLKLGFNFSFPCHQTGLDRSTLISWTKGFRCSGVEGQDVVQLLRDAIQRQGTYRIDVVAMVNDTVGTMMGCELGTRPCEVGLIVDTGTNACYMEEARHVAALDEDRGRTCVSIEWGSFYDEDALGPVLTTFDSALDRESLTPGAQRFEKMIGGLYLGELVRLVLVHLTQHGVLFDGCASPALLSQGCILLDHVAEMEDTATGTARVHTILQDLGLSPRASDAELVQYVCVAVCTRAAQLCAAALAAVLSRLQHSREQQTLQVAVATGGRVFERHPRFLRILKETVTLLAPNCDVSFIPSVDGGGRGVAMVTAVAARLAAHRRILEETLAPFQLTLEQMTVVQAQMREAMIRGLQGEASSLRMLPTYVRATPDGSERGDFLALDLGGTNFRVLLVRVAEGSVQIINQVYSIPECRAQGSGQKLFDHIVDCIVDFQKRQGLSGQSLPLGFTFSFPCKQLGLDQGILLNWTKGFNASGCEGQDVVYLLREAIRRRQAVELNVVAIVNDTVGTMMSCGYDDPRCEMGLIVGTGTNACYMEELRNVASVPGDSGLMCINMEWGAFGDDGSLGTLSTRFDTSVDQASINPGKQRFEKMISGMYLGEIVRHILLHLTNLGVLFRGQKTQCLQARDIFKTKFLSEIESDSLALRQVRAILEDLGLTLTSDDALMVLEVCQAVSRRAAQLCGAGVAAVVEKIRENRGLQELTVSVGVDGTLYKLHPHFSKLVSATVRKLAPQCTVTFLQSEDGSGKGAALVTAVACRLTQMAHV

Target ORF information:

RefSeq Version	NM_001206392.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus hexokinase 3 (Hk3), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206392.1

ORF Insert Sequence:

ATGGAGCAGG CACTGAAGGG ACAGGACAGT CCCGCTCCTT CTGTCCGGAT GCTGCCCACA 
TACGTGAGGT CCACACCACA CGGCACCGAG CAAGGGGATT TCCTGGTGCT GGAGCTGGGG 
GCCACAGGAG CATCACTACG TGTGTTGTGG GTAACACTGA CAGGCACCAA AGAATGCCGG 
GTGGAGCCCA GGAGCCGGGA GTTTGTGATT CCTCAAGAGG TGATCCTAGG TGCTGGCCAG 
CAGCTCTTTG ACTTTGCTGC CCGATGCCTC TCTGAATTCC TGGATGCATA CCCCGTGGAG 
AATCAGGGTC TGAAGCTTGG GTTCAATTTC TCTTTTCCTT GTCACCAGAC AGGCTTGGAC 
AGGAGCACCC TCATTTCCTG GACAAAAGGT TTTAGGTGCA GCGGCGTGGA AGGCCAGGAT 
GTGGTCCAGT TGCTAAGAGA TGCCATTCAG AGGCAGGGGA CCTACAGGAT TGATGTGGTA 
GCCATGGTGA ATGACACGGT GGGCACCATG ATGGGCTGTG AGCTGGGCAC CAGGCCGTGT 
GAAGTCGGGC TGATTGTAGA CACCGGTACC AACGCGTGCT ACATGGAGGA GGCGCGGCAC 
GTGGCCGCTC TGGATGAGGA CCGCGGCCGC ACCTGTGTCA GCATCGAGTG GGGCTCCTTC 
TATGACGAAG ATGCTCTAGG GCCAGTACTG ACCACCTTCG ACTCTGCCCT GGACCGCGAG 
TCCCTGACTC CTGGTGCTCA AAGGTTTGAG AAGATGATTG GAGGCCTTTA CCTGGGTGAG 
CTGGTGAGGC TGGTTCTGGT CCACTTGACC CAGCACGGGG TCCTCTTTGA TGGCTGCGCC 
TCTCCTGCGT TGCTGAGTCA AGGCTGTATC CTCCTGGACC ACGTGGCTGA GATGGAGGAC 
ACCGCCACTG GGACAGCCCG CGTCCACACC ATCCTGCAGG ACTTGGGTCT GAGCCCTCGG 
GCCTCAGATG CTGAACTGGT GCAATATGTG TGCGTGGCTG TGTGCACACG GGCTGCCCAG 
CTCTGTGCGG CTGCCCTGGC TGCAGTCCTC TCCCGCCTCC AGCACAGCAG GGAGCAGCAG 
ACATTGCAAG TGGCTGTGGC CACTGGAGGG CGAGTGTTTG AACGACACCC CAGGTTCCTC 
CGAATCCTAA AGGAGACGGT AACACTCCTG GCCCCAAACT GTGATGTCTC CTTCATCCCC 
TCTGTGGATG GTGGTGGCCG GGGTGTGGCA ATGGTGACAG CTGTGGCTGC CCGCCTGGCT 
GCTCACAGGC GCATCCTGGA AGAGACCCTG GCACCATTTC AGCTGACCTT GGAGCAGATG 
ACAGTGGTGC AGGCACAAAT GCGGGAAGCC ATGATCAGGG GGCTTCAAGG AGAGGCCTCC 
TCCCTCCGCA TGCTGCCCAC TTACGTCCGA GCGACGCCCG ACGGCAGCGA GCGAGGTGAT 
TTCCTGGCCT TGGACCTAGG GGGCACCAAC TTCCGGGTCC TGTTGGTACG AGTGGCGGAG 
GGCAGTGTGC AGATCATCAA CCAGGTCTAC TCCATTCCTG AGTGTAGAGC CCAGGGCTCT 
GGACAGAAGC TCTTTGACCA TATTGTGGAC TGCATCGTGG ACTTCCAGAA GAGGCAGGGC 
CTTAGTGGAC AGAGCCTACC CCTGGGTTTC ACCTTCTCTT TCCCATGCAA GCAGCTTGGC 
CTGGACCAGG GCATCCTCCT GAATTGGACT AAGGGGTTCA ATGCATCAGG CTGTGAGGGC 
CAGGATGTTG TGTATCTATT ACGGGAAGCC ATTAGGCGCA GACAGGCAGT GGAGCTGAAT 
GTGGTTGCCA TTGTCAATGA CACGGTGGGG ACCATGATGT CCTGTGGCTA TGATGATCCC 
CGTTGTGAGA TGGGCCTCAT TGTCGGAACC GGCACCAACG CCTGTTACAT GGAAGAGCTC 
CGCAATGTGG CAAGTGTGCC TGGGGACTCG GGCCTCATGT GCATCAACAT GGAGTGGGGT 
GCCTTTGGGG ATGATGGCTC ACTGGGCACA CTCAGCACCC GCTTTGACAC CAGTGTGGAC 
CAGGCATCCA TCAATCCAGG CAAACAGAGG TTTGAGAAAA TGATCAGCGG TATGTACTTG 
GGGGAGATCG TCCGCCATAT CCTCCTGCAC TTAACCAATC TCGGAGTTCT CTTCCGAGGC 
CAGAAGACTC AATGCCTTCA GGCCAGGGAC ATCTTCAAGA CTAAGTTCCT CTCTGAGATC 
GAAAGTGATA GCCTGGCCCT GCGTCAGGTC CGAGCCATCC TGGAAGATCT GGGGCTGACT 
CTGACATCTG ATGACGCCTT GATGGTCCTG GAAGTGTGCC AGGCTGTGTC TCGCAGGGCT 
GCCCAACTCT GTGGGGCAGG TGTGGCTGCC GTGGTGGAGA AGATTCGGGA GAACCGGGGC 
CTGCAGGAGC TGACAGTGTC TGTGGGAGTA GATGGGACAC TCTACAAGCT ACATCCTCAC 
TTCTCCAAGC TGGTGTCGGC AACAGTCCGG AAGCTAGCCC CTCAGTGCAC CGTCACCTTT 
TTGCAGTCGG AGGATGGGTC TGGCAAAGGC GCAGCTTTGG TTACTGCTGT TGCTTGTCGC 
CTTACCCAGA TGGCCCACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hk3 ( NM_001206391.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001193320.1 Mus musculus hexokinase 3 (Hk3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu12903
Clone ID Related Accession (Same CDS sequence)	NM_001206391.1
Accession Version	NM_001206391.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	hexokinase-3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154610.3. Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate in-frame splice site in the coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC117861.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGCCACCA TTGGGCCTTC TGGCCTGCAT CCTGGAGAAA GAGCCTCAGT CTGCCCACAC 
GAGGGCGTGC CAAGGCCCTC AGGTAGCTTA GAACTGGAAT GCTTGCAGCA ATTCAAGGTG 
ACCAGGACAC AGTTGCAGCA GATCCAAGCC AGTCTCCTGT GCTCCATGGA GCAGGCACTG 
AAGGGACAGG ACAGTCCCGC TCCTTCTGTC CGGATGCTGC CCACATACGT GAGGTCCACA 
CCACACGGCA CCGAGCAAGG GGATTTCCTG GTGCTGGAGC TGGGGGCCAC AGGAGCATCA 
CTACGTGTGT TGTGGGTAAC ACTGACAGGC ACCAAAGAAT GCCGGGTGGA GCCCAGGAGC 
CGGGAGTTTG TGATTCCTCA AGAGGTGATC CTAGGTGCTG GCCAGCAGCT CTTTGACTTT 
GCTGCCCGAT GCCTCTCTGA ATTCCTGGAT GCATACCCCG TGGAGAATCA GGGTCTGAAG 
CTTGGGTTCA ATTTCTCTTT TCCTTGTCAC CAGACAGGCT TGGACAGGAG CACCCTCATT 
TCCTGGACAA AAGGTTTTAG GTGCAGCGGC GTGGAAGGCC AGGATGTGGT CCAGTTGCTA 
AGAGATGCCA TTCAGAGGCA GGGGACCTAC AGGATTGATG TGGTAGCCAT GGTGAATGAC 
ACGGTGGGCA CCATGATGGG CTGTGAGCTG GGCACCAGGC CGTGTGAAGT CGGGCTGATT 
GTAGACACCG GTACCAACGC GTGCTACATG GAGGAGGCGC GGCACGTGGC CGCTCTGGAT 
GAGGACCGCG GCCGCACCTG TGTCAGCATC GAGTGGGGCT CCTTCTATGA CGAAGATGCT 
CTAGGGCCAG TACTGACCAC CTTCGACTCT GCCCTGGACC GCGAGTCCCT GACTCCTGGT 
GCTCAAAGGT TTGAGAAGAT GATTGGAGGC CTTTACCTGG GTGAGCTGGT GAGGCTGGTT 
CTGGTCCACT TGACCCAGCA CGGGGTCCTC TTTGATGGCT GCGCCTCTCC TGCGTTGCTG 
AGTCAAGGCT GTATCCTCCT GGACCACGTG GCTGAGATGG AGGACACCGC CACTGGGACA 
GCCCGCGTCC ACACCATCCT GCAGGACTTG GGTCTGAGCC CTCGGGCCTC AGATGCTGAA 
CTGGTGCAAT ATGTGTGCGT GGCTGTGTGC ACACGGGCTG CCCAGCTCTG TGCGGCTGCC 
CTGGCTGCAG TCCTCTCCCG CCTCCAGCAC AGCAGGGAGC AGCAGACATT GCAAGTGGCT 
GTGGCCACTG GAGGGCGAGT GTTTGAACGA CACCCCAGGC GCATCCTGGA AGAGACCCTG 
GCACCATTTC AGCTGACCTT GGAGCAGATG ACAGTGGTGC AGGCACAAAT GCGGGAAGCC 
ATGATCAGGG GGCTTCAAGG AGAGGCCTCC TCCCTCCGCA TGCTGCCCAC TTACGTCCGA 
GCGACGCCCG ACGGCAGCGA GCGAGGTGAT TTCCTGGCCT TGGACCTAGG GGGCACCAAC 
TTCCGGGTCC TGTTGGTACG AGTGGCGGAG GGCAGTGTGC AGATCATCAA CCAGGTCTAC 
TCCATTCCTG AGTGTAGAGC CCAGGGCTCT GGACAGAAGC TCTTTGACCA TATTGTGGAC 
TGCATCGTGG ACTTCCAGAA GAGGCAGGGC CTTAGTGGAC AGAGCCTACC CCTGGGTTTC 
ACCTTCTCTT TCCCATGCAA GCAGCTTGGC CTGGACCAGG GCATCCTCCT GAATTGGACT 
AAGGGGTTCA ATGCATCAGG CTGTGAGGGC CAGGATGTTG TGTATCTATT ACGGGAAGCC 
ATTAGGCGCA GACAGGCAGT GGAGCTGAAT GTGGTTGCCA TTGTCAATGA CACGGTGGGG 
ACCATGATGT CCTGTGGCTA TGATGATCCC CGTTGTGAGA TGGGCCTCAT TGTCGGAACC 
GGCACCAACG CCTGTTACAT GGAAGAGCTC CGCAATGTGG CAAGTGTGCC TGGGGACTCG 
GGCCTCATGT GCATCAACAT GGAGTGGGGT GCCTTTGGGG ATGATGGCTC ACTGGGCACA 
CTCAGCACCC GCTTTGACAC CAGTGTGGAC CAGGCATCCA TCAATCCAGG CAAACAGAGG 
TTTGAGAAAA TGATCAGCGG TATGTACTTG GGGGAGATCG TCCGCCATAT CCTCCTGCAC 
TTAACCAATC TCGGAGTTCT CTTCCGAGGC CAGAAGACTC AATGCCTTCA GGCCAGGGAC 
ATCTTCAAGA CTAAGTTCCT CTCTGAGATC GAAAGTGATA GCCTGGCCCT GCGTCAGGTC 
CGAGCCATCC TGGAAGATCT GGGGCTGACT CTGACATCTG ATGACGCCTT GATGGTCCTG 
GAAGTGTGCC AGGCTGTGTC TCGCAGGGCT GCCCAACTCT GTGGGGCAGG TGTGGCTGCC 
GTGGTGGAGA AGATTCGGGA GAACCGGGGC CTGCAGGAGC TGACAGTGTC TGTGGGAGTA 
GATGGGACAC TCTACAAGCT ACATCCTCAC TTCTCCAAGC TGGTGTCGGC AACAGTCCGG 
AAGCTAGCCC CTCAGTGCAC CGTCACCTTT TTGCAGTCGG AGGATGGGTC TGGCAAAGGC 
GCAGCTTTGG TTACTGCTGT TGCTTGTCGC CTTACCCAGA TGGCCCACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193320.1
CDS	75..2708
Translation	MATIGPSGLHPGERASVCPHEGVPRPSGSLELECLQQFKVTRTQLQQIQASLLCSMEQALKGQDSPAPSVRMLPTYVRSTPHGTEQGDFLVLELGATGASLRVLWVTLTGTKECRVEPRSREFVIPQEVILGAGQQLFDFAARCLSEFLDAYPVENQGLKLGFNFSFPCHQTGLDRSTLISWTKGFRCSGVEGQDVVQLLRDAIQRQGTYRIDVVAMVNDTVGTMMGCELGTRPCEVGLIVDTGTNACYMEEARHVAALDEDRGRTCVSIEWGSFYDEDALGPVLTTFDSALDRESLTPGAQRFEKMIGGLYLGELVRLVLVHLTQHGVLFDGCASPALLSQGCILLDHVAEMEDTATGTARVHTILQDLGLSPRASDAELVQYVCVAVCTRAAQLCAAALAAVLSRLQHSREQQTLQVAVATGGRVFERHPRRILEETLAPFQLTLEQMTVVQAQMREAMIRGLQGEASSLRMLPTYVRATPDGSERGDFLALDLGGTNFRVLLVRVAEGSVQIINQVYSIPECRAQGSGQKLFDHIVDCIVDFQKRQGLSGQSLPLGFTFSFPCKQLGLDQGILLNWTKGFNASGCEGQDVVYLLREAIRRRQAVELNVVAIVNDTVGTMMSCGYDDPRCEMGLIVGTGTNACYMEELRNVASVPGDSGLMCINMEWGAFGDDGSLGTLSTRFDTSVDQASINPGKQRFEKMISGMYLGEIVRHILLHLTNLGVLFRGQKTQCLQARDIFKTKFLSEIESDSLALRQVRAILEDLGLTLTSDDALMVLEVCQAVSRRAAQLCGAGVAAVVEKIRENRGLQELTVSVGVDGTLYKLHPHFSKLVSATVRKLAPQCTVTFLQSEDGSGKGAALVTAVACRLTQMAHV

Target ORF information:

RefSeq Version	NM_001206391.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus hexokinase 3 (Hk3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206391.1

ORF Insert Sequence:

ATGGCCACCA TTGGGCCTTC TGGCCTGCAT CCTGGAGAAA GAGCCTCAGT CTGCCCACAC 
GAGGGCGTGC CAAGGCCCTC AGGTAGCTTA GAACTGGAAT GCTTGCAGCA ATTCAAGGTG 
ACCAGGACAC AGTTGCAGCA GATCCAAGCC AGTCTCCTGT GCTCCATGGA GCAGGCACTG 
AAGGGACAGG ACAGTCCCGC TCCTTCTGTC CGGATGCTGC CCACATACGT GAGGTCCACA 
CCACACGGCA CCGAGCAAGG GGATTTCCTG GTGCTGGAGC TGGGGGCCAC AGGAGCATCA 
CTACGTGTGT TGTGGGTAAC ACTGACAGGC ACCAAAGAAT GCCGGGTGGA GCCCAGGAGC 
CGGGAGTTTG TGATTCCTCA AGAGGTGATC CTAGGTGCTG GCCAGCAGCT CTTTGACTTT 
GCTGCCCGAT GCCTCTCTGA ATTCCTGGAT GCATACCCCG TGGAGAATCA GGGTCTGAAG 
CTTGGGTTCA ATTTCTCTTT TCCTTGTCAC CAGACAGGCT TGGACAGGAG CACCCTCATT 
TCCTGGACAA AAGGTTTTAG GTGCAGCGGC GTGGAAGGCC AGGATGTGGT CCAGTTGCTA 
AGAGATGCCA TTCAGAGGCA GGGGACCTAC AGGATTGATG TGGTAGCCAT GGTGAATGAC 
ACGGTGGGCA CCATGATGGG CTGTGAGCTG GGCACCAGGC CGTGTGAAGT CGGGCTGATT 
GTAGACACCG GTACCAACGC GTGCTACATG GAGGAGGCGC GGCACGTGGC CGCTCTGGAT 
GAGGACCGCG GCCGCACCTG TGTCAGCATC GAGTGGGGCT CCTTCTATGA CGAAGATGCT 
CTAGGGCCAG TACTGACCAC CTTCGACTCT GCCCTGGACC GCGAGTCCCT GACTCCTGGT 
GCTCAAAGGT TTGAGAAGAT GATTGGAGGC CTTTACCTGG GTGAGCTGGT GAGGCTGGTT 
CTGGTCCACT TGACCCAGCA CGGGGTCCTC TTTGATGGCT GCGCCTCTCC TGCGTTGCTG 
AGTCAAGGCT GTATCCTCCT GGACCACGTG GCTGAGATGG AGGACACCGC CACTGGGACA 
GCCCGCGTCC ACACCATCCT GCAGGACTTG GGTCTGAGCC CTCGGGCCTC AGATGCTGAA 
CTGGTGCAAT ATGTGTGCGT GGCTGTGTGC ACACGGGCTG CCCAGCTCTG TGCGGCTGCC 
CTGGCTGCAG TCCTCTCCCG CCTCCAGCAC AGCAGGGAGC AGCAGACATT GCAAGTGGCT 
GTGGCCACTG GAGGGCGAGT GTTTGAACGA CACCCCAGGC GCATCCTGGA AGAGACCCTG 
GCACCATTTC AGCTGACCTT GGAGCAGATG ACAGTGGTGC AGGCACAAAT GCGGGAAGCC 
ATGATCAGGG GGCTTCAAGG AGAGGCCTCC TCCCTCCGCA TGCTGCCCAC TTACGTCCGA 
GCGACGCCCG ACGGCAGCGA GCGAGGTGAT TTCCTGGCCT TGGACCTAGG GGGCACCAAC 
TTCCGGGTCC TGTTGGTACG AGTGGCGGAG GGCAGTGTGC AGATCATCAA CCAGGTCTAC 
TCCATTCCTG AGTGTAGAGC CCAGGGCTCT GGACAGAAGC TCTTTGACCA TATTGTGGAC 
TGCATCGTGG ACTTCCAGAA GAGGCAGGGC CTTAGTGGAC AGAGCCTACC CCTGGGTTTC 
ACCTTCTCTT TCCCATGCAA GCAGCTTGGC CTGGACCAGG GCATCCTCCT GAATTGGACT 
AAGGGGTTCA ATGCATCAGG CTGTGAGGGC CAGGATGTTG TGTATCTATT ACGGGAAGCC 
ATTAGGCGCA GACAGGCAGT GGAGCTGAAT GTGGTTGCCA TTGTCAATGA CACGGTGGGG 
ACCATGATGT CCTGTGGCTA TGATGATCCC CGTTGTGAGA TGGGCCTCAT TGTCGGAACC 
GGCACCAACG CCTGTTACAT GGAAGAGCTC CGCAATGTGG CAAGTGTGCC TGGGGACTCG 
GGCCTCATGT GCATCAACAT GGAGTGGGGT GCCTTTGGGG ATGATGGCTC ACTGGGCACA 
CTCAGCACCC GCTTTGACAC CAGTGTGGAC CAGGCATCCA TCAATCCAGG CAAACAGAGG 
TTTGAGAAAA TGATCAGCGG TATGTACTTG GGGGAGATCG TCCGCCATAT CCTCCTGCAC 
TTAACCAATC TCGGAGTTCT CTTCCGAGGC CAGAAGACTC AATGCCTTCA GGCCAGGGAC 
ATCTTCAAGA CTAAGTTCCT CTCTGAGATC GAAAGTGATA GCCTGGCCCT GCGTCAGGTC 
CGAGCCATCC TGGAAGATCT GGGGCTGACT CTGACATCTG ATGACGCCTT GATGGTCCTG 
GAAGTGTGCC AGGCTGTGTC TCGCAGGGCT GCCCAACTCT GTGGGGCAGG TGTGGCTGCC 
GTGGTGGAGA AGATTCGGGA GAACCGGGGC CTGCAGGAGC TGACAGTGTC TGTGGGAGTA 
GATGGGACAC TCTACAAGCT ACATCCTCAC TTCTCCAAGC TGGTGTCGGC AACAGTCCGG 
AAGCTAGCCC CTCAGTGCAC CGTCACCTTT TTGCAGTCGG AGGATGGGTC TGGCAAAGGC 
GCAGCTTTGG TTACTGCTGT TGCTTGTCGC CTTACCCAGA TGGCCCACGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hk3 ( NM_001033245.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001028417.1 Mus musculus hexokinase 3 (Hk3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu12949
Clone ID Related Accession (Same CDS sequence)	NM_001033245.4 , NM_001206390.1
Accession Version	NM_001033245.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	hexokinase-3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154610.3, AK171580.1 and BI133441.1. On Apr 22, 2011 this sequence version replaced NM_001033245.3. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK171580.1, AK154603.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGCCACCA TTGGGCCTTC TGGCCTGCAT CCTGGAGAAA GAGCCTCAGT CTGCCCACAC 
GAGGGCGTGC CAAGGCCCTC AGGTAGCTTA GAACTGGAAT GCTTGCAGCA ATTCAAGGTG 
ACCAGGACAC AGTTGCAGCA GATCCAAGCC AGTCTCCTGT GCTCCATGGA GCAGGCACTG 
AAGGGACAGG ACAGTCCCGC TCCTTCTGTC CGGATGCTGC CCACATACGT GAGGTCCACA 
CCACACGGCA CCGAGCAAGG GGATTTCCTG GTGCTGGAGC TGGGGGCCAC AGGAGCATCA 
CTACGTGTGT TGTGGGTAAC ACTGACAGGC ACCAAAGAAT GCCGGGTGGA GCCCAGGAGC 
CGGGAGTTTG TGATTCCTCA AGAGGTGATC CTAGGTGCTG GCCAGCAGCT CTTTGACTTT 
GCTGCCCGAT GCCTCTCTGA ATTCCTGGAT GCATACCCCG TGGAGAATCA GGGTCTGAAG 
CTTGGGTTCA ATTTCTCTTT TCCTTGTCAC CAGACAGGCT TGGACAGGAG CACCCTCATT 
TCCTGGACAA AAGGTTTTAG GTGCAGCGGC GTGGAAGGCC AGGATGTGGT CCAGTTGCTA 
AGAGATGCCA TTCAGAGGCA GGGGACCTAC AGGATTGATG TGGTAGCCAT GGTGAATGAC 
ACGGTGGGCA CCATGATGGG CTGTGAGCTG GGCACCAGGC CGTGTGAAGT CGGGCTGATT 
GTAGACACCG GTACCAACGC GTGCTACATG GAGGAGGCGC GGCACGTGGC CGCTCTGGAT 
GAGGACCGCG GCCGCACCTG TGTCAGCATC GAGTGGGGCT CCTTCTATGA CGAAGATGCT 
CTAGGGCCAG TACTGACCAC CTTCGACTCT GCCCTGGACC GCGAGTCCCT GACTCCTGGT 
GCTCAAAGGT TTGAGAAGAT GATTGGAGGC CTTTACCTGG GTGAGCTGGT GAGGCTGGTT 
CTGGTCCACT TGACCCAGCA CGGGGTCCTC TTTGATGGCT GCGCCTCTCC TGCGTTGCTG 
AGTCAAGGCT GTATCCTCCT GGACCACGTG GCTGAGATGG AGGACACCGC CACTGGGACA 
GCCCGCGTCC ACACCATCCT GCAGGACTTG GGTCTGAGCC CTCGGGCCTC AGATGCTGAA 
CTGGTGCAAT ATGTGTGCGT GGCTGTGTGC ACACGGGCTG CCCAGCTCTG TGCGGCTGCC 
CTGGCTGCAG TCCTCTCCCG CCTCCAGCAC AGCAGGGAGC AGCAGACATT GCAAGTGGCT 
GTGGCCACTG GAGGGCGAGT GTTTGAACGA CACCCCAGGT TCCTCCGAAT CCTAAAGGAG 
ACGGTAACAC TCCTGGCCCC AAACTGTGAT GTCTCCTTCA TCCCCTCTGT GGATGGTGGT 
GGCCGGGGTG TGGCAATGGT GACAGCTGTG GCTGCCCGCC TGGCTGCTCA CAGGCGCATC 
CTGGAAGAGA CCCTGGCACC ATTTCAGCTG ACCTTGGAGC AGATGACAGT GGTGCAGGCA 
CAAATGCGGG AAGCCATGAT CAGGGGGCTT CAAGGAGAGG CCTCCTCCCT CCGCATGCTG 
CCCACTTACG TCCGAGCGAC GCCCGACGGC AGCGAGCGAG GTGATTTCCT GGCCTTGGAC 
CTAGGGGGCA CCAACTTCCG GGTCCTGTTG GTACGAGTGG CGGAGGGCAG TGTGCAGATC 
ATCAACCAGG TCTACTCCAT TCCTGAGTGT AGAGCCCAGG GCTCTGGACA GAAGCTCTTT 
GACCATATTG TGGACTGCAT CGTGGACTTC CAGAAGAGGC AGGGCCTTAG TGGACAGAGC 
CTACCCCTGG GTTTCACCTT CTCTTTCCCA TGCAAGCAGC TTGGCCTGGA CCAGGGCATC 
CTCCTGAATT GGACTAAGGG GTTCAATGCA TCAGGCTGTG AGGGCCAGGA TGTTGTGTAT 
CTATTACGGG AAGCCATTAG GCGCAGACAG GCAGTGGAGC TGAATGTGGT TGCCATTGTC 
AATGACACGG TGGGGACCAT GATGTCCTGT GGCTATGATG ATCCCCGTTG TGAGATGGGC 
CTCATTGTCG GAACCGGCAC CAACGCCTGT TACATGGAAG AGCTCCGCAA TGTGGCAAGT 
GTGCCTGGGG ACTCGGGCCT CATGTGCATC AACATGGAGT GGGGTGCCTT TGGGGATGAT 
GGCTCACTGG GCACACTCAG CACCCGCTTT GACACCAGTG TGGACCAGGC ATCCATCAAT 
CCAGGCAAAC AGAGGTTTGA GAAAATGATC AGCGGTATGT ACTTGGGGGA GATCGTCCGC 
CATATCCTCC TGCACTTAAC CAATCTCGGA GTTCTCTTCC GAGGCCAGAA GACTCAATGC 
CTTCAGGCCA GGGACATCTT CAAGACTAAG TTCCTCTCTG AGATCGAAAG TGATAGCCTG 
GCCCTGCGTC AGGTCCGAGC CATCCTGGAA GATCTGGGGC TGACTCTGAC ATCTGATGAC 
GCCTTGATGG TCCTGGAAGT GTGCCAGGCT GTGTCTCGCA GGGCTGCCCA ACTCTGTGGG 
GCAGGTGTGG CTGCCGTGGT GGAGAAGATT CGGGAGAACC GGGGCCTGCA GGAGCTGACA 
GTGTCTGTGG GAGTAGATGG GACACTCTAC AAGCTACATC CTCACTTCTC CAAGCTGGTG 
TCGGCAACAG TCCGGAAGCT AGCCCCTCAG TGCACCGTCA CCTTTTTGCA GTCGGAGGAT 
GGGTCTGGCA AAGGCGCAGC TTTGGTTACT GCTGTTGCTT GTCGCCTTAC CCAGATGGCC 
CACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028417.1
CDS	265..3033
Translation	MATIGPSGLHPGERASVCPHEGVPRPSGSLELECLQQFKVTRTQLQQIQASLLCSMEQALKGQDSPAPSVRMLPTYVRSTPHGTEQGDFLVLELGATGASLRVLWVTLTGTKECRVEPRSREFVIPQEVILGAGQQLFDFAARCLSEFLDAYPVENQGLKLGFNFSFPCHQTGLDRSTLISWTKGFRCSGVEGQDVVQLLRDAIQRQGTYRIDVVAMVNDTVGTMMGCELGTRPCEVGLIVDTGTNACYMEEARHVAALDEDRGRTCVSIEWGSFYDEDALGPVLTTFDSALDRESLTPGAQRFEKMIGGLYLGELVRLVLVHLTQHGVLFDGCASPALLSQGCILLDHVAEMEDTATGTARVHTILQDLGLSPRASDAELVQYVCVAVCTRAAQLCAAALAAVLSRLQHSREQQTLQVAVATGGRVFERHPRFLRILKETVTLLAPNCDVSFIPSVDGGGRGVAMVTAVAARLAAHRRILEETLAPFQLTLEQMTVVQAQMREAMIRGLQGEASSLRMLPTYVRATPDGSERGDFLALDLGGTNFRVLLVRVAEGSVQIINQVYSIPECRAQGSGQKLFDHIVDCIVDFQKRQGLSGQSLPLGFTFSFPCKQLGLDQGILLNWTKGFNASGCEGQDVVYLLREAIRRRQAVELNVVAIVNDTVGTMMSCGYDDPRCEMGLIVGTGTNACYMEELRNVASVPGDSGLMCINMEWGAFGDDGSLGTLSTRFDTSVDQASINPGKQRFEKMISGMYLGEIVRHILLHLTNLGVLFRGQKTQCLQARDIFKTKFLSEIESDSLALRQVRAILEDLGLTLTSDDALMVLEVCQAVSRRAAQLCGAGVAAVVEKIRENRGLQELTVSVGVDGTLYKLHPHFSKLVSATVRKLAPQCTVTFLQSEDGSGKGAALVTAVACRLTQMAHV

Target ORF information:

RefSeq Version	NM_001033245.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus hexokinase 3 (Hk3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033245.4

ORF Insert Sequence:

ATGGCCACCA TTGGGCCTTC TGGCCTGCAT CCTGGAGAAA GAGCCTCAGT CTGCCCACAC 
GAGGGCGTGC CAAGGCCCTC AGGTAGCTTA GAACTGGAAT GCTTGCAGCA ATTCAAGGTG 
ACCAGGACAC AGTTGCAGCA GATCCAAGCC AGTCTCCTGT GCTCCATGGA GCAGGCACTG 
AAGGGACAGG ACAGTCCCGC TCCTTCTGTC CGGATGCTGC CCACATACGT GAGGTCCACA 
CCACACGGCA CCGAGCAAGG GGATTTCCTG GTGCTGGAGC TGGGGGCCAC AGGAGCATCA 
CTACGTGTGT TGTGGGTAAC ACTGACAGGC ACCAAAGAAT GCCGGGTGGA GCCCAGGAGC 
CGGGAGTTTG TGATTCCTCA AGAGGTGATC CTAGGTGCTG GCCAGCAGCT CTTTGACTTT 
GCTGCCCGAT GCCTCTCTGA ATTCCTGGAT GCATACCCCG TGGAGAATCA GGGTCTGAAG 
CTTGGGTTCA ATTTCTCTTT TCCTTGTCAC CAGACAGGCT TGGACAGGAG CACCCTCATT 
TCCTGGACAA AAGGTTTTAG GTGCAGCGGC GTGGAAGGCC AGGATGTGGT CCAGTTGCTA 
AGAGATGCCA TTCAGAGGCA GGGGACCTAC AGGATTGATG TGGTAGCCAT GGTGAATGAC 
ACGGTGGGCA CCATGATGGG CTGTGAGCTG GGCACCAGGC CGTGTGAAGT CGGGCTGATT 
GTAGACACCG GTACCAACGC GTGCTACATG GAGGAGGCGC GGCACGTGGC CGCTCTGGAT 
GAGGACCGCG GCCGCACCTG TGTCAGCATC GAGTGGGGCT CCTTCTATGA CGAAGATGCT 
CTAGGGCCAG TACTGACCAC CTTCGACTCT GCCCTGGACC GCGAGTCCCT GACTCCTGGT 
GCTCAAAGGT TTGAGAAGAT GATTGGAGGC CTTTACCTGG GTGAGCTGGT GAGGCTGGTT 
CTGGTCCACT TGACCCAGCA CGGGGTCCTC TTTGATGGCT GCGCCTCTCC TGCGTTGCTG 
AGTCAAGGCT GTATCCTCCT GGACCACGTG GCTGAGATGG AGGACACCGC CACTGGGACA 
GCCCGCGTCC ACACCATCCT GCAGGACTTG GGTCTGAGCC CTCGGGCCTC AGATGCTGAA 
CTGGTGCAAT ATGTGTGCGT GGCTGTGTGC ACACGGGCTG CCCAGCTCTG TGCGGCTGCC 
CTGGCTGCAG TCCTCTCCCG CCTCCAGCAC AGCAGGGAGC AGCAGACATT GCAAGTGGCT 
GTGGCCACTG GAGGGCGAGT GTTTGAACGA CACCCCAGGT TCCTCCGAAT CCTAAAGGAG 
ACGGTAACAC TCCTGGCCCC AAACTGTGAT GTCTCCTTCA TCCCCTCTGT GGATGGTGGT 
GGCCGGGGTG TGGCAATGGT GACAGCTGTG GCTGCCCGCC TGGCTGCTCA CAGGCGCATC 
CTGGAAGAGA CCCTGGCACC ATTTCAGCTG ACCTTGGAGC AGATGACAGT GGTGCAGGCA 
CAAATGCGGG AAGCCATGAT CAGGGGGCTT CAAGGAGAGG CCTCCTCCCT CCGCATGCTG 
CCCACTTACG TCCGAGCGAC GCCCGACGGC AGCGAGCGAG GTGATTTCCT GGCCTTGGAC 
CTAGGGGGCA CCAACTTCCG GGTCCTGTTG GTACGAGTGG CGGAGGGCAG TGTGCAGATC 
ATCAACCAGG TCTACTCCAT TCCTGAGTGT AGAGCCCAGG GCTCTGGACA GAAGCTCTTT 
GACCATATTG TGGACTGCAT CGTGGACTTC CAGAAGAGGC AGGGCCTTAG TGGACAGAGC 
CTACCCCTGG GTTTCACCTT CTCTTTCCCA TGCAAGCAGC TTGGCCTGGA CCAGGGCATC 
CTCCTGAATT GGACTAAGGG GTTCAATGCA TCAGGCTGTG AGGGCCAGGA TGTTGTGTAT 
CTATTACGGG AAGCCATTAG GCGCAGACAG GCAGTGGAGC TGAATGTGGT TGCCATTGTC 
AATGACACGG TGGGGACCAT GATGTCCTGT GGCTATGATG ATCCCCGTTG TGAGATGGGC 
CTCATTGTCG GAACCGGCAC CAACGCCTGT TACATGGAAG AGCTCCGCAA TGTGGCAAGT 
GTGCCTGGGG ACTCGGGCCT CATGTGCATC AACATGGAGT GGGGTGCCTT TGGGGATGAT 
GGCTCACTGG GCACACTCAG CACCCGCTTT GACACCAGTG TGGACCAGGC ATCCATCAAT 
CCAGGCAAAC AGAGGTTTGA GAAAATGATC AGCGGTATGT ACTTGGGGGA GATCGTCCGC 
CATATCCTCC TGCACTTAAC CAATCTCGGA GTTCTCTTCC GAGGCCAGAA GACTCAATGC 
CTTCAGGCCA GGGACATCTT CAAGACTAAG TTCCTCTCTG AGATCGAAAG TGATAGCCTG 
GCCCTGCGTC AGGTCCGAGC CATCCTGGAA GATCTGGGGC TGACTCTGAC ATCTGATGAC 
GCCTTGATGG TCCTGGAAGT GTGCCAGGCT GTGTCTCGCA GGGCTGCCCA ACTCTGTGGG 
GCAGGTGTGG CTGCCGTGGT GGAGAAGATT CGGGAGAACC GGGGCCTGCA GGAGCTGACA 
GTGTCTGTGG GAGTAGATGG GACACTCTAC AAGCTACATC CTCACTTCTC CAAGCTGGTG 
TCGGCAACAG TCCGGAAGCT AGCCCCTCAG TGCACCGTCA CCTTTTTGCA GTCGGAGGAT 
GGGTCTGGCA AAGGCGCAGC TTTGGTTACT GCTGTTGCTT GTCGCCTTAC CCAGATGGCC 
CACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hk3 ( NM_001206390.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001193319.1 Mus musculus hexokinase 3 (Hk3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu12949
Clone ID Related Accession (Same CDS sequence)	NM_001033245.4 , NM_001206390.1
Accession Version	NM_001206390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	hexokinase-3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154610.3. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC117860.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGCCACCA TTGGGCCTTC TGGCCTGCAT CCTGGAGAAA GAGCCTCAGT CTGCCCACAC 
GAGGGCGTGC CAAGGCCCTC AGGTAGCTTA GAACTGGAAT GCTTGCAGCA ATTCAAGGTG 
ACCAGGACAC AGTTGCAGCA GATCCAAGCC AGTCTCCTGT GCTCCATGGA GCAGGCACTG 
AAGGGACAGG ACAGTCCCGC TCCTTCTGTC CGGATGCTGC CCACATACGT GAGGTCCACA 
CCACACGGCA CCGAGCAAGG GGATTTCCTG GTGCTGGAGC TGGGGGCCAC AGGAGCATCA 
CTACGTGTGT TGTGGGTAAC ACTGACAGGC ACCAAAGAAT GCCGGGTGGA GCCCAGGAGC 
CGGGAGTTTG TGATTCCTCA AGAGGTGATC CTAGGTGCTG GCCAGCAGCT CTTTGACTTT 
GCTGCCCGAT GCCTCTCTGA ATTCCTGGAT GCATACCCCG TGGAGAATCA GGGTCTGAAG 
CTTGGGTTCA ATTTCTCTTT TCCTTGTCAC CAGACAGGCT TGGACAGGAG CACCCTCATT 
TCCTGGACAA AAGGTTTTAG GTGCAGCGGC GTGGAAGGCC AGGATGTGGT CCAGTTGCTA 
AGAGATGCCA TTCAGAGGCA GGGGACCTAC AGGATTGATG TGGTAGCCAT GGTGAATGAC 
ACGGTGGGCA CCATGATGGG CTGTGAGCTG GGCACCAGGC CGTGTGAAGT CGGGCTGATT 
GTAGACACCG GTACCAACGC GTGCTACATG GAGGAGGCGC GGCACGTGGC CGCTCTGGAT 
GAGGACCGCG GCCGCACCTG TGTCAGCATC GAGTGGGGCT CCTTCTATGA CGAAGATGCT 
CTAGGGCCAG TACTGACCAC CTTCGACTCT GCCCTGGACC GCGAGTCCCT GACTCCTGGT 
GCTCAAAGGT TTGAGAAGAT GATTGGAGGC CTTTACCTGG GTGAGCTGGT GAGGCTGGTT 
CTGGTCCACT TGACCCAGCA CGGGGTCCTC TTTGATGGCT GCGCCTCTCC TGCGTTGCTG 
AGTCAAGGCT GTATCCTCCT GGACCACGTG GCTGAGATGG AGGACACCGC CACTGGGACA 
GCCCGCGTCC ACACCATCCT GCAGGACTTG GGTCTGAGCC CTCGGGCCTC AGATGCTGAA 
CTGGTGCAAT ATGTGTGCGT GGCTGTGTGC ACACGGGCTG CCCAGCTCTG TGCGGCTGCC 
CTGGCTGCAG TCCTCTCCCG CCTCCAGCAC AGCAGGGAGC AGCAGACATT GCAAGTGGCT 
GTGGCCACTG GAGGGCGAGT GTTTGAACGA CACCCCAGGT TCCTCCGAAT CCTAAAGGAG 
ACGGTAACAC TCCTGGCCCC AAACTGTGAT GTCTCCTTCA TCCCCTCTGT GGATGGTGGT 
GGCCGGGGTG TGGCAATGGT GACAGCTGTG GCTGCCCGCC TGGCTGCTCA CAGGCGCATC 
CTGGAAGAGA CCCTGGCACC ATTTCAGCTG ACCTTGGAGC AGATGACAGT GGTGCAGGCA 
CAAATGCGGG AAGCCATGAT CAGGGGGCTT CAAGGAGAGG CCTCCTCCCT CCGCATGCTG 
CCCACTTACG TCCGAGCGAC GCCCGACGGC AGCGAGCGAG GTGATTTCCT GGCCTTGGAC 
CTAGGGGGCA CCAACTTCCG GGTCCTGTTG GTACGAGTGG CGGAGGGCAG TGTGCAGATC 
ATCAACCAGG TCTACTCCAT TCCTGAGTGT AGAGCCCAGG GCTCTGGACA GAAGCTCTTT 
GACCATATTG TGGACTGCAT CGTGGACTTC CAGAAGAGGC AGGGCCTTAG TGGACAGAGC 
CTACCCCTGG GTTTCACCTT CTCTTTCCCA TGCAAGCAGC TTGGCCTGGA CCAGGGCATC 
CTCCTGAATT GGACTAAGGG GTTCAATGCA TCAGGCTGTG AGGGCCAGGA TGTTGTGTAT 
CTATTACGGG AAGCCATTAG GCGCAGACAG GCAGTGGAGC TGAATGTGGT TGCCATTGTC 
AATGACACGG TGGGGACCAT GATGTCCTGT GGCTATGATG ATCCCCGTTG TGAGATGGGC 
CTCATTGTCG GAACCGGCAC CAACGCCTGT TACATGGAAG AGCTCCGCAA TGTGGCAAGT 
GTGCCTGGGG ACTCGGGCCT CATGTGCATC AACATGGAGT GGGGTGCCTT TGGGGATGAT 
GGCTCACTGG GCACACTCAG CACCCGCTTT GACACCAGTG TGGACCAGGC ATCCATCAAT 
CCAGGCAAAC AGAGGTTTGA GAAAATGATC AGCGGTATGT ACTTGGGGGA GATCGTCCGC 
CATATCCTCC TGCACTTAAC CAATCTCGGA GTTCTCTTCC GAGGCCAGAA GACTCAATGC 
CTTCAGGCCA GGGACATCTT CAAGACTAAG TTCCTCTCTG AGATCGAAAG TGATAGCCTG 
GCCCTGCGTC AGGTCCGAGC CATCCTGGAA GATCTGGGGC TGACTCTGAC ATCTGATGAC 
GCCTTGATGG TCCTGGAAGT GTGCCAGGCT GTGTCTCGCA GGGCTGCCCA ACTCTGTGGG 
GCAGGTGTGG CTGCCGTGGT GGAGAAGATT CGGGAGAACC GGGGCCTGCA GGAGCTGACA 
GTGTCTGTGG GAGTAGATGG GACACTCTAC AAGCTACATC CTCACTTCTC CAAGCTGGTG 
TCGGCAACAG TCCGGAAGCT AGCCCCTCAG TGCACCGTCA CCTTTTTGCA GTCGGAGGAT 
GGGTCTGGCA AAGGCGCAGC TTTGGTTACT GCTGTTGCTT GTCGCCTTAC CCAGATGGCC 
CACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193319.1
CDS	75..2843
Translation	MATIGPSGLHPGERASVCPHEGVPRPSGSLELECLQQFKVTRTQLQQIQASLLCSMEQALKGQDSPAPSVRMLPTYVRSTPHGTEQGDFLVLELGATGASLRVLWVTLTGTKECRVEPRSREFVIPQEVILGAGQQLFDFAARCLSEFLDAYPVENQGLKLGFNFSFPCHQTGLDRSTLISWTKGFRCSGVEGQDVVQLLRDAIQRQGTYRIDVVAMVNDTVGTMMGCELGTRPCEVGLIVDTGTNACYMEEARHVAALDEDRGRTCVSIEWGSFYDEDALGPVLTTFDSALDRESLTPGAQRFEKMIGGLYLGELVRLVLVHLTQHGVLFDGCASPALLSQGCILLDHVAEMEDTATGTARVHTILQDLGLSPRASDAELVQYVCVAVCTRAAQLCAAALAAVLSRLQHSREQQTLQVAVATGGRVFERHPRFLRILKETVTLLAPNCDVSFIPSVDGGGRGVAMVTAVAARLAAHRRILEETLAPFQLTLEQMTVVQAQMREAMIRGLQGEASSLRMLPTYVRATPDGSERGDFLALDLGGTNFRVLLVRVAEGSVQIINQVYSIPECRAQGSGQKLFDHIVDCIVDFQKRQGLSGQSLPLGFTFSFPCKQLGLDQGILLNWTKGFNASGCEGQDVVYLLREAIRRRQAVELNVVAIVNDTVGTMMSCGYDDPRCEMGLIVGTGTNACYMEELRNVASVPGDSGLMCINMEWGAFGDDGSLGTLSTRFDTSVDQASINPGKQRFEKMISGMYLGEIVRHILLHLTNLGVLFRGQKTQCLQARDIFKTKFLSEIESDSLALRQVRAILEDLGLTLTSDDALMVLEVCQAVSRRAAQLCGAGVAAVVEKIRENRGLQELTVSVGVDGTLYKLHPHFSKLVSATVRKLAPQCTVTFLQSEDGSGKGAALVTAVACRLTQMAHV

Target ORF information:

RefSeq Version	NM_001206390.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus hexokinase 3 (Hk3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206390.1

ORF Insert Sequence:

ATGGCCACCA TTGGGCCTTC TGGCCTGCAT CCTGGAGAAA GAGCCTCAGT CTGCCCACAC 
GAGGGCGTGC CAAGGCCCTC AGGTAGCTTA GAACTGGAAT GCTTGCAGCA ATTCAAGGTG 
ACCAGGACAC AGTTGCAGCA GATCCAAGCC AGTCTCCTGT GCTCCATGGA GCAGGCACTG 
AAGGGACAGG ACAGTCCCGC TCCTTCTGTC CGGATGCTGC CCACATACGT GAGGTCCACA 
CCACACGGCA CCGAGCAAGG GGATTTCCTG GTGCTGGAGC TGGGGGCCAC AGGAGCATCA 
CTACGTGTGT TGTGGGTAAC ACTGACAGGC ACCAAAGAAT GCCGGGTGGA GCCCAGGAGC 
CGGGAGTTTG TGATTCCTCA AGAGGTGATC CTAGGTGCTG GCCAGCAGCT CTTTGACTTT 
GCTGCCCGAT GCCTCTCTGA ATTCCTGGAT GCATACCCCG TGGAGAATCA GGGTCTGAAG 
CTTGGGTTCA ATTTCTCTTT TCCTTGTCAC CAGACAGGCT TGGACAGGAG CACCCTCATT 
TCCTGGACAA AAGGTTTTAG GTGCAGCGGC GTGGAAGGCC AGGATGTGGT CCAGTTGCTA 
AGAGATGCCA TTCAGAGGCA GGGGACCTAC AGGATTGATG TGGTAGCCAT GGTGAATGAC 
ACGGTGGGCA CCATGATGGG CTGTGAGCTG GGCACCAGGC CGTGTGAAGT CGGGCTGATT 
GTAGACACCG GTACCAACGC GTGCTACATG GAGGAGGCGC GGCACGTGGC CGCTCTGGAT 
GAGGACCGCG GCCGCACCTG TGTCAGCATC GAGTGGGGCT CCTTCTATGA CGAAGATGCT 
CTAGGGCCAG TACTGACCAC CTTCGACTCT GCCCTGGACC GCGAGTCCCT GACTCCTGGT 
GCTCAAAGGT TTGAGAAGAT GATTGGAGGC CTTTACCTGG GTGAGCTGGT GAGGCTGGTT 
CTGGTCCACT TGACCCAGCA CGGGGTCCTC TTTGATGGCT GCGCCTCTCC TGCGTTGCTG 
AGTCAAGGCT GTATCCTCCT GGACCACGTG GCTGAGATGG AGGACACCGC CACTGGGACA 
GCCCGCGTCC ACACCATCCT GCAGGACTTG GGTCTGAGCC CTCGGGCCTC AGATGCTGAA 
CTGGTGCAAT ATGTGTGCGT GGCTGTGTGC ACACGGGCTG CCCAGCTCTG TGCGGCTGCC 
CTGGCTGCAG TCCTCTCCCG CCTCCAGCAC AGCAGGGAGC AGCAGACATT GCAAGTGGCT 
GTGGCCACTG GAGGGCGAGT GTTTGAACGA CACCCCAGGT TCCTCCGAAT CCTAAAGGAG 
ACGGTAACAC TCCTGGCCCC AAACTGTGAT GTCTCCTTCA TCCCCTCTGT GGATGGTGGT 
GGCCGGGGTG TGGCAATGGT GACAGCTGTG GCTGCCCGCC TGGCTGCTCA CAGGCGCATC 
CTGGAAGAGA CCCTGGCACC ATTTCAGCTG ACCTTGGAGC AGATGACAGT GGTGCAGGCA 
CAAATGCGGG AAGCCATGAT CAGGGGGCTT CAAGGAGAGG CCTCCTCCCT CCGCATGCTG 
CCCACTTACG TCCGAGCGAC GCCCGACGGC AGCGAGCGAG GTGATTTCCT GGCCTTGGAC 
CTAGGGGGCA CCAACTTCCG GGTCCTGTTG GTACGAGTGG CGGAGGGCAG TGTGCAGATC 
ATCAACCAGG TCTACTCCAT TCCTGAGTGT AGAGCCCAGG GCTCTGGACA GAAGCTCTTT 
GACCATATTG TGGACTGCAT CGTGGACTTC CAGAAGAGGC AGGGCCTTAG TGGACAGAGC 
CTACCCCTGG GTTTCACCTT CTCTTTCCCA TGCAAGCAGC TTGGCCTGGA CCAGGGCATC 
CTCCTGAATT GGACTAAGGG GTTCAATGCA TCAGGCTGTG AGGGCCAGGA TGTTGTGTAT 
CTATTACGGG AAGCCATTAG GCGCAGACAG GCAGTGGAGC TGAATGTGGT TGCCATTGTC 
AATGACACGG TGGGGACCAT GATGTCCTGT GGCTATGATG ATCCCCGTTG TGAGATGGGC 
CTCATTGTCG GAACCGGCAC CAACGCCTGT TACATGGAAG AGCTCCGCAA TGTGGCAAGT 
GTGCCTGGGG ACTCGGGCCT CATGTGCATC AACATGGAGT GGGGTGCCTT TGGGGATGAT 
GGCTCACTGG GCACACTCAG CACCCGCTTT GACACCAGTG TGGACCAGGC ATCCATCAAT 
CCAGGCAAAC AGAGGTTTGA GAAAATGATC AGCGGTATGT ACTTGGGGGA GATCGTCCGC 
CATATCCTCC TGCACTTAAC CAATCTCGGA GTTCTCTTCC GAGGCCAGAA GACTCAATGC 
CTTCAGGCCA GGGACATCTT CAAGACTAAG TTCCTCTCTG AGATCGAAAG TGATAGCCTG 
GCCCTGCGTC AGGTCCGAGC CATCCTGGAA GATCTGGGGC TGACTCTGAC ATCTGATGAC 
GCCTTGATGG TCCTGGAAGT GTGCCAGGCT GTGTCTCGCA GGGCTGCCCA ACTCTGTGGG 
GCAGGTGTGG CTGCCGTGGT GGAGAAGATT CGGGAGAACC GGGGCCTGCA GGAGCTGACA 
GTGTCTGTGG GAGTAGATGG GACACTCTAC AAGCTACATC CTCACTTCTC CAAGCTGGTG 
TCGGCAACAG TCCGGAAGCT AGCCCCTCAG TGCACCGTCA CCTTTTTGCA GTCGGAGGAT 
GGGTCTGGCA AAGGCGCAGC TTTGGTTACT GCTGTTGCTT GTCGCCTTAC CCAGATGGCC 
CACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hk3 ( XM_006517193.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517256.1 Mus musculus hexokinase 3 (Hk3), transcript variant X1, mRNA.

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CloneID	OMu48717
Clone ID Related Accession (Same CDS sequence)	XM_006517193.3 , XM_006517193.4
Accession Version	XM_006517193.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	hexokinase-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517193.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATGCT TGCAGCAATT CAAGGTGACC AGGACACAGT TGCAGCAGAT CCAAGCCAGT 
CTCCTGTGCT CCATGGAGCA GGCACTGAAG GGACAGGACA GTCCCGCTCC TTCTGTCCGG 
ATGCTGCCCA CATACGTGAG GTCCACACCA CACGGCACCG AGCAAGGGGA TTTCCTGGTG 
CTGGAGCTGG GGGCCACAGG AGCATCACTA CGTGTGTTGT GGGTAACACT GACAGGCACC 
AAAGAATGCC GGGTGGAGCC CAGGAGCCGG GAGTTTGTGA TTCCTCAAGA GGTGATCCTA 
GGTGCTGGCC AGCAGCTCTT TGACTTTGCT GCCCGATGCC TCTCTGAATT CCTGGATGCA 
TACCCCGTGG AGAATCAGGG TCTGAAGCTT GGGTTCAATT TCTCTTTTCC TTGTCACCAG 
ACAGGCTTGG ACAGGAGCAC CCTCATTTCC TGGACAAAAG GTTTTAGGTG CAGCGGCGTG 
GAAGGCCAGG ATGTGGTCCA GTTGCTAAGA GATGCCATTC AGAGGCAGGG GACCTACAGG 
ATTGATGTGG TAGCCATGGT GAATGACACG GTGGGCACCA TGATGGGCTG TGAGCTGGGC 
ACCAGGCCGT GTGAAGTCGG GCTGATTGTA GACACCGGTA CCAACGCGTG CTACATGGAG 
GAGGCGCGGC ACGTGGCCGC TCTGGATGAG GACCGCGGCC GCACCTGTGT CAGCATCGAG 
TGGGGCTCCT TCTATGACGA AGATGCTCTA GGGCCAGTAC TGACCACCTT CGACTCTGCC 
CTGGACCGCG AGTCCCTGAC TCCTGGTGCT CAAAGGTTTG AGAAGATGAT TGGAGGCCTT 
TACCTGGGTG AGCTGGTGAG GCTGGTTCTG GTCCACTTGA CCCAGCACGG GGTCCTCTTT 
GATGGCTGCG CCTCTCCTGC GTTGCTGAGT CAAGGCTGTA TCCTCCTGGA CCACGTGGCT 
GAGATGGAGG ACACCGCCAC TGGGACAGCC CGCGTCCACA CCATCCTGCA GGACTTGGGT 
CTGAGCCCTC GGGCCTCAGA TGCTGAACTG GTGCAATATG TGTGCGTGGC TGTGTGCACA 
CGGGCTGCCC AGCTCTGTGC GGCTGCCCTG GCTGCAGTCC TCTCCCGCCT CCAGCACAGC 
AGGGAGCAGC AGACATTGCA AGTGGCTGTG GCCACTGGAG GGCGAGTGTT TGAACGACAC 
CCCAGGTTCC TCCGAATCCT AAAGGAGACG GTAACACTCC TGGCCCCAAA CTGTGATGTC 
TCCTTCATCC CCTCTGTGGA TGGTGGTGGC CGGGGTGTGG CAATGGTGAC AGCTGTGGCT 
GCCCGCCTGG CTGCTCACAG GCGCATCCTG GAAGAGACCC TGGCACCATT TCAGCTGACC 
TTGGAGCAGA TGACAGTGGT GCAGGCACAA ATGCGGGAAG CCATGATCAG GGGGCTTCAA 
GGAGAGGCCT CCTCCCTCCG CATGCTGCCC ACTTACGTCC GAGCGACGCC CGACGGCAGC 
GAGCGAGGTG ATTTCCTGGC CTTGGACCTA GGGGGCACCA ACTTCCGGGT CCTGTTGGTA 
CGAGTGGCGG AGGGCAGTGT GCAGATCATC AACCAGGTCT ACTCCATTCC TGAGTGTAGA 
GCCCAGGGCT CTGGACAGAA GCTCTTTGAC CATATTGTGG ACTGCATCGT GGACTTCCAG 
AAGAGGCAGG GCCTTAGTGG ACAGAGCCTA CCCCTGGGTT TCACCTTCTC TTTCCCATGC 
AAGCAGCTTG GCCTGGACCA GGGCATCCTC CTGAATTGGA CTAAGGGGTT CAATGCATCA 
GGCTGTGAGG GCCAGGATGT TGTGTATCTA TTACGGGAAG CCATTAGGCG CAGACAGGCA 
GTGGAGCTGA ATGTGGTTGC CATTGTCAAT GACACGGTGG GGACCATGAT GTCCTGTGGC 
TATGATGATC CCCGTTGTGA GATGGGCCTC ATTGTCGGAA CCGGCACCAA CGCCTGTTAC 
ATGGAAGAGC TCCGCAATGT GGCAAGTGTG CCTGGGGACT CGGGCCTCAT GTGCATCAAC 
ATGGAGTGGG GTGCCTTTGG GGATGATGGC TCACTGGGCA CACTCAGCAC CCGCTTTGAC 
ACCAGTGTGG ACCAGGCATC CATCAATCCA GGCAAACAGA GGTTTGAGAA AATGATCAGC 
GGTATGTACT TGGGGGAGAT CGTCCGCCAT ATCCTCCTGC ACTTAACCAA TCTCGGAGTT 
CTCTTCCGAG GCCAGAAGAC TCAATGCCTT CAGGCCAGGG ACATCTTCAA GACTAAGTTC 
CTCTCTGAGA TCGAAAGTGA TAGCCTGGCC CTGCGTCAGG TCCGAGCCAT CCTGGAAGAT 
CTGGGGCTGA CTCTGACATC TGATGACGCC TTGATGGTCC TGGAAGTGTG CCAGGCTGTG 
TCTCGCAGGG CTGCCCAACT CTGTGGGGCA GGTGTGGCTG CCGTGGTGGA GAAGATTCGG 
GAGAACCGGG GCCTGCAGGA GCTGACAGTG TCTGTGGGAG TAGATGGGAC ACTCTACAAG 
CTACATCCTC ACTTCTCCAA GCTGGTGTCG GCAACAGTCC GGAAGCTAGC CCCTCAGTGC 
ACCGTCACCT TTTTGCAGTC GGAGGATGGG TCTGGCAAAG GCGCAGCTTT GGTTACTGCT 
GTTGCTTGTC GCCTTACCCA GATGGCCCAC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517256.1
CDS	541..3216
Translation	MECLQQFKVTRTQLQQIQASLLCSMEQALKGQDSPAPSVRMLPTYVRSTPHGTEQGDFLVLELGATGASLRVLWVTLTGTKECRVEPRSREFVIPQEVILGAGQQLFDFAARCLSEFLDAYPVENQGLKLGFNFSFPCHQTGLDRSTLISWTKGFRCSGVEGQDVVQLLRDAIQRQGTYRIDVVAMVNDTVGTMMGCELGTRPCEVGLIVDTGTNACYMEEARHVAALDEDRGRTCVSIEWGSFYDEDALGPVLTTFDSALDRESLTPGAQRFEKMIGGLYLGELVRLVLVHLTQHGVLFDGCASPALLSQGCILLDHVAEMEDTATGTARVHTILQDLGLSPRASDAELVQYVCVAVCTRAAQLCAAALAAVLSRLQHSREQQTLQVAVATGGRVFERHPRFLRILKETVTLLAPNCDVSFIPSVDGGGRGVAMVTAVAARLAAHRRILEETLAPFQLTLEQMTVVQAQMREAMIRGLQGEASSLRMLPTYVRATPDGSERGDFLALDLGGTNFRVLLVRVAEGSVQIINQVYSIPECRAQGSGQKLFDHIVDCIVDFQKRQGLSGQSLPLGFTFSFPCKQLGLDQGILLNWTKGFNASGCEGQDVVYLLREAIRRRQAVELNVVAIVNDTVGTMMSCGYDDPRCEMGLIVGTGTNACYMEELRNVASVPGDSGLMCINMEWGAFGDDGSLGTLSTRFDTSVDQASINPGKQRFEKMISGMYLGEIVRHILLHLTNLGVLFRGQKTQCLQARDIFKTKFLSEIESDSLALRQVRAILEDLGLTLTSDDALMVLEVCQAVSRRAAQLCGAGVAAVVEKIRENRGLQELTVSVGVDGTLYKLHPHFSKLVSATVRKLAPQCTVTFLQSEDGSGKGAALVTAVACRLTQMAHV

Target ORF information:

RefSeq Version	XM_006517193.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus hexokinase 3 (Hk3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517193.3

ORF Insert Sequence:

ATGGAATGCT TGCAGCAATT CAAGGTGACC AGGACACAGT TGCAGCAGAT CCAAGCCAGT 
CTCCTGTGCT CCATGGAGCA GGCACTGAAG GGACAGGACA GTCCCGCTCC TTCTGTCCGG 
ATGCTGCCCA CATACGTGAG GTCCACACCA CACGGCACCG AGCAAGGGGA TTTCCTGGTG 
CTGGAGCTGG GGGCCACAGG AGCATCACTA CGTGTGTTGT GGGTAACACT GACAGGCACC 
AAAGAATGCC GGGTGGAGCC CAGGAGCCGG GAGTTTGTGA TTCCTCAAGA GGTGATCCTA 
GGTGCTGGCC AGCAGCTCTT TGACTTTGCT GCCCGATGCC TCTCTGAATT CCTGGATGCA 
TACCCCGTGG AGAATCAGGG TCTGAAGCTT GGGTTCAATT TCTCTTTTCC TTGTCACCAG 
ACAGGCTTGG ACAGGAGCAC CCTCATTTCC TGGACAAAAG GTTTTAGGTG CAGCGGCGTG 
GAAGGCCAGG ATGTGGTCCA GTTGCTAAGA GATGCCATTC AGAGGCAGGG GACCTACAGG 
ATTGATGTGG TAGCCATGGT GAATGACACG GTGGGCACCA TGATGGGCTG TGAGCTGGGC 
ACCAGGCCGT GTGAAGTCGG GCTGATTGTA GACACCGGTA CCAACGCGTG CTACATGGAG 
GAGGCGCGGC ACGTGGCCGC TCTGGATGAG GACCGCGGCC GCACCTGTGT CAGCATCGAG 
TGGGGCTCCT TCTATGACGA AGATGCTCTA GGGCCAGTAC TGACCACCTT CGACTCTGCC 
CTGGACCGCG AGTCCCTGAC TCCTGGTGCT CAAAGGTTTG AGAAGATGAT TGGAGGCCTT 
TACCTGGGTG AGCTGGTGAG GCTGGTTCTG GTCCACTTGA CCCAGCACGG GGTCCTCTTT 
GATGGCTGCG CCTCTCCTGC GTTGCTGAGT CAAGGCTGTA TCCTCCTGGA CCACGTGGCT 
GAGATGGAGG ACACCGCCAC TGGGACAGCC CGCGTCCACA CCATCCTGCA GGACTTGGGT 
CTGAGCCCTC GGGCCTCAGA TGCTGAACTG GTGCAATATG TGTGCGTGGC TGTGTGCACA 
CGGGCTGCCC AGCTCTGTGC GGCTGCCCTG GCTGCAGTCC TCTCCCGCCT CCAGCACAGC 
AGGGAGCAGC AGACATTGCA AGTGGCTGTG GCCACTGGAG GGCGAGTGTT TGAACGACAC 
CCCAGGTTCC TCCGAATCCT AAAGGAGACG GTAACACTCC TGGCCCCAAA CTGTGATGTC 
TCCTTCATCC CCTCTGTGGA TGGTGGTGGC CGGGGTGTGG CAATGGTGAC AGCTGTGGCT 
GCCCGCCTGG CTGCTCACAG GCGCATCCTG GAAGAGACCC TGGCACCATT TCAGCTGACC 
TTGGAGCAGA TGACAGTGGT GCAGGCACAA ATGCGGGAAG CCATGATCAG GGGGCTTCAA 
GGAGAGGCCT CCTCCCTCCG CATGCTGCCC ACTTACGTCC GAGCGACGCC CGACGGCAGC 
GAGCGAGGTG ATTTCCTGGC CTTGGACCTA GGGGGCACCA ACTTCCGGGT CCTGTTGGTA 
CGAGTGGCGG AGGGCAGTGT GCAGATCATC AACCAGGTCT ACTCCATTCC TGAGTGTAGA 
GCCCAGGGCT CTGGACAGAA GCTCTTTGAC CATATTGTGG ACTGCATCGT GGACTTCCAG 
AAGAGGCAGG GCCTTAGTGG ACAGAGCCTA CCCCTGGGTT TCACCTTCTC TTTCCCATGC 
AAGCAGCTTG GCCTGGACCA GGGCATCCTC CTGAATTGGA CTAAGGGGTT CAATGCATCA 
GGCTGTGAGG GCCAGGATGT TGTGTATCTA TTACGGGAAG CCATTAGGCG CAGACAGGCA 
GTGGAGCTGA ATGTGGTTGC CATTGTCAAT GACACGGTGG GGACCATGAT GTCCTGTGGC 
TATGATGATC CCCGTTGTGA GATGGGCCTC ATTGTCGGAA CCGGCACCAA CGCCTGTTAC 
ATGGAAGAGC TCCGCAATGT GGCAAGTGTG CCTGGGGACT CGGGCCTCAT GTGCATCAAC 
ATGGAGTGGG GTGCCTTTGG GGATGATGGC TCACTGGGCA CACTCAGCAC CCGCTTTGAC 
ACCAGTGTGG ACCAGGCATC CATCAATCCA GGCAAACAGA GGTTTGAGAA AATGATCAGC 
GGTATGTACT TGGGGGAGAT CGTCCGCCAT ATCCTCCTGC ACTTAACCAA TCTCGGAGTT 
CTCTTCCGAG GCCAGAAGAC TCAATGCCTT CAGGCCAGGG ACATCTTCAA GACTAAGTTC 
CTCTCTGAGA TCGAAAGTGA TAGCCTGGCC CTGCGTCAGG TCCGAGCCAT CCTGGAAGAT 
CTGGGGCTGA CTCTGACATC TGATGACGCC TTGATGGTCC TGGAAGTGTG CCAGGCTGTG 
TCTCGCAGGG CTGCCCAACT CTGTGGGGCA GGTGTGGCTG CCGTGGTGGA GAAGATTCGG 
GAGAACCGGG GCCTGCAGGA GCTGACAGTG TCTGTGGGAG TAGATGGGAC ACTCTACAAG 
CTACATCCTC ACTTCTCCAA GCTGGTGTCG GCAACAGTCC GGAAGCTAGC CCCTCAGTGC 
ACCGTCACCT TTTTGCAGTC GGAGGATGGG TCTGGCAAAG GCGCAGCTTT GGTTACTGCT 
GTTGCTTGTC GCCTTACCCA GATGGCCCAC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hk3 ( XM_006517193.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517256.1 Mus musculus hexokinase 3 (Hk3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu48717
Clone ID Related Accession (Same CDS sequence)	XM_006517193.3 , XM_006517193.4
Accession Version	XM_006517193.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	hexokinase-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006517193.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATGCT TGCAGCAATT CAAGGTGACC AGGACACAGT TGCAGCAGAT CCAAGCCAGT 
CTCCTGTGCT CCATGGAGCA GGCACTGAAG GGACAGGACA GTCCCGCTCC TTCTGTCCGG 
ATGCTGCCCA CATACGTGAG GTCCACACCA CACGGCACCG AGCAAGGGGA TTTCCTGGTG 
CTGGAGCTGG GGGCCACAGG AGCATCACTA CGTGTGTTGT GGGTAACACT GACAGGCACC 
AAAGAATGCC GGGTGGAGCC CAGGAGCCGG GAGTTTGTGA TTCCTCAAGA GGTGATCCTA 
GGTGCTGGCC AGCAGCTCTT TGACTTTGCT GCCCGATGCC TCTCTGAATT CCTGGATGCA 
TACCCCGTGG AGAATCAGGG TCTGAAGCTT GGGTTCAATT TCTCTTTTCC TTGTCACCAG 
ACAGGCTTGG ACAGGAGCAC CCTCATTTCC TGGACAAAAG GTTTTAGGTG CAGCGGCGTG 
GAAGGCCAGG ATGTGGTCCA GTTGCTAAGA GATGCCATTC AGAGGCAGGG GACCTACAGG 
ATTGATGTGG TAGCCATGGT GAATGACACG GTGGGCACCA TGATGGGCTG TGAGCTGGGC 
ACCAGGCCGT GTGAAGTCGG GCTGATTGTA GACACCGGTA CCAACGCGTG CTACATGGAG 
GAGGCGCGGC ACGTGGCCGC TCTGGATGAG GACCGCGGCC GCACCTGTGT CAGCATCGAG 
TGGGGCTCCT TCTATGACGA AGATGCTCTA GGGCCAGTAC TGACCACCTT CGACTCTGCC 
CTGGACCGCG AGTCCCTGAC TCCTGGTGCT CAAAGGTTTG AGAAGATGAT TGGAGGCCTT 
TACCTGGGTG AGCTGGTGAG GCTGGTTCTG GTCCACTTGA CCCAGCACGG GGTCCTCTTT 
GATGGCTGCG CCTCTCCTGC GTTGCTGAGT CAAGGCTGTA TCCTCCTGGA CCACGTGGCT 
GAGATGGAGG ACACCGCCAC TGGGACAGCC CGCGTCCACA CCATCCTGCA GGACTTGGGT 
CTGAGCCCTC GGGCCTCAGA TGCTGAACTG GTGCAATATG TGTGCGTGGC TGTGTGCACA 
CGGGCTGCCC AGCTCTGTGC GGCTGCCCTG GCTGCAGTCC TCTCCCGCCT CCAGCACAGC 
AGGGAGCAGC AGACATTGCA AGTGGCTGTG GCCACTGGAG GGCGAGTGTT TGAACGACAC 
CCCAGGTTCC TCCGAATCCT AAAGGAGACG GTAACACTCC TGGCCCCAAA CTGTGATGTC 
TCCTTCATCC CCTCTGTGGA TGGTGGTGGC CGGGGTGTGG CAATGGTGAC AGCTGTGGCT 
GCCCGCCTGG CTGCTCACAG GCGCATCCTG GAAGAGACCC TGGCACCATT TCAGCTGACC 
TTGGAGCAGA TGACAGTGGT GCAGGCACAA ATGCGGGAAG CCATGATCAG GGGGCTTCAA 
GGAGAGGCCT CCTCCCTCCG CATGCTGCCC ACTTACGTCC GAGCGACGCC CGACGGCAGC 
GAGCGAGGTG ATTTCCTGGC CTTGGACCTA GGGGGCACCA ACTTCCGGGT CCTGTTGGTA 
CGAGTGGCGG AGGGCAGTGT GCAGATCATC AACCAGGTCT ACTCCATTCC TGAGTGTAGA 
GCCCAGGGCT CTGGACAGAA GCTCTTTGAC CATATTGTGG ACTGCATCGT GGACTTCCAG 
AAGAGGCAGG GCCTTAGTGG ACAGAGCCTA CCCCTGGGTT TCACCTTCTC TTTCCCATGC 
AAGCAGCTTG GCCTGGACCA GGGCATCCTC CTGAATTGGA CTAAGGGGTT CAATGCATCA 
GGCTGTGAGG GCCAGGATGT TGTGTATCTA TTACGGGAAG CCATTAGGCG CAGACAGGCA 
GTGGAGCTGA ATGTGGTTGC CATTGTCAAT GACACGGTGG GGACCATGAT GTCCTGTGGC 
TATGATGATC CCCGTTGTGA GATGGGCCTC ATTGTCGGAA CCGGCACCAA CGCCTGTTAC 
ATGGAAGAGC TCCGCAATGT GGCAAGTGTG CCTGGGGACT CGGGCCTCAT GTGCATCAAC 
ATGGAGTGGG GTGCCTTTGG GGATGATGGC TCACTGGGCA CACTCAGCAC CCGCTTTGAC 
ACCAGTGTGG ACCAGGCATC CATCAATCCA GGCAAACAGA GGTTTGAGAA AATGATCAGC 
GGTATGTACT TGGGGGAGAT CGTCCGCCAT ATCCTCCTGC ACTTAACCAA TCTCGGAGTT 
CTCTTCCGAG GCCAGAAGAC TCAATGCCTT CAGGCCAGGG ACATCTTCAA GACTAAGTTC 
CTCTCTGAGA TCGAAAGTGA TAGCCTGGCC CTGCGTCAGG TCCGAGCCAT CCTGGAAGAT 
CTGGGGCTGA CTCTGACATC TGATGACGCC TTGATGGTCC TGGAAGTGTG CCAGGCTGTG 
TCTCGCAGGG CTGCCCAACT CTGTGGGGCA GGTGTGGCTG CCGTGGTGGA GAAGATTCGG 
GAGAACCGGG GCCTGCAGGA GCTGACAGTG TCTGTGGGAG TAGATGGGAC ACTCTACAAG 
CTACATCCTC ACTTCTCCAA GCTGGTGTCG GCAACAGTCC GGAAGCTAGC CCCTCAGTGC 
ACCGTCACCT TTTTGCAGTC GGAGGATGGG TCTGGCAAAG GCGCAGCTTT GGTTACTGCT 
GTTGCTTGTC GCCTTACCCA GATGGCCCAC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517256.1
CDS	540..3215
Translation	MECLQQFKVTRTQLQQIQASLLCSMEQALKGQDSPAPSVRMLPTYVRSTPHGTEQGDFLVLELGATGASLRVLWVTLTGTKECRVEPRSREFVIPQEVILGAGQQLFDFAARCLSEFLDAYPVENQGLKLGFNFSFPCHQTGLDRSTLISWTKGFRCSGVEGQDVVQLLRDAIQRQGTYRIDVVAMVNDTVGTMMGCELGTRPCEVGLIVDTGTNACYMEEARHVAALDEDRGRTCVSIEWGSFYDEDALGPVLTTFDSALDRESLTPGAQRFEKMIGGLYLGELVRLVLVHLTQHGVLFDGCASPALLSQGCILLDHVAEMEDTATGTARVHTILQDLGLSPRASDAELVQYVCVAVCTRAAQLCAAALAAVLSRLQHSREQQTLQVAVATGGRVFERHPRFLRILKETVTLLAPNCDVSFIPSVDGGGRGVAMVTAVAARLAAHRRILEETLAPFQLTLEQMTVVQAQMREAMIRGLQGEASSLRMLPTYVRATPDGSERGDFLALDLGGTNFRVLLVRVAEGSVQIINQVYSIPECRAQGSGQKLFDHIVDCIVDFQKRQGLSGQSLPLGFTFSFPCKQLGLDQGILLNWTKGFNASGCEGQDVVYLLREAIRRRQAVELNVVAIVNDTVGTMMSCGYDDPRCEMGLIVGTGTNACYMEELRNVASVPGDSGLMCINMEWGAFGDDGSLGTLSTRFDTSVDQASINPGKQRFEKMISGMYLGEIVRHILLHLTNLGVLFRGQKTQCLQARDIFKTKFLSEIESDSLALRQVRAILEDLGLTLTSDDALMVLEVCQAVSRRAAQLCGAGVAAVVEKIRENRGLQELTVSVGVDGTLYKLHPHFSKLVSATVRKLAPQCTVTFLQSEDGSGKGAALVTAVACRLTQMAHV

Target ORF information:

RefSeq Version	XM_006517193.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus hexokinase 3 (Hk3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517193.4

ORF Insert Sequence:

ATGGAATGCT TGCAGCAATT CAAGGTGACC AGGACACAGT TGCAGCAGAT CCAAGCCAGT 
CTCCTGTGCT CCATGGAGCA GGCACTGAAG GGACAGGACA GTCCCGCTCC TTCTGTCCGG 
ATGCTGCCCA CATACGTGAG GTCCACACCA CACGGCACCG AGCAAGGGGA TTTCCTGGTG 
CTGGAGCTGG GGGCCACAGG AGCATCACTA CGTGTGTTGT GGGTAACACT GACAGGCACC 
AAAGAATGCC GGGTGGAGCC CAGGAGCCGG GAGTTTGTGA TTCCTCAAGA GGTGATCCTA 
GGTGCTGGCC AGCAGCTCTT TGACTTTGCT GCCCGATGCC TCTCTGAATT CCTGGATGCA 
TACCCCGTGG AGAATCAGGG TCTGAAGCTT GGGTTCAATT TCTCTTTTCC TTGTCACCAG 
ACAGGCTTGG ACAGGAGCAC CCTCATTTCC TGGACAAAAG GTTTTAGGTG CAGCGGCGTG 
GAAGGCCAGG ATGTGGTCCA GTTGCTAAGA GATGCCATTC AGAGGCAGGG GACCTACAGG 
ATTGATGTGG TAGCCATGGT GAATGACACG GTGGGCACCA TGATGGGCTG TGAGCTGGGC 
ACCAGGCCGT GTGAAGTCGG GCTGATTGTA GACACCGGTA CCAACGCGTG CTACATGGAG 
GAGGCGCGGC ACGTGGCCGC TCTGGATGAG GACCGCGGCC GCACCTGTGT CAGCATCGAG 
TGGGGCTCCT TCTATGACGA AGATGCTCTA GGGCCAGTAC TGACCACCTT CGACTCTGCC 
CTGGACCGCG AGTCCCTGAC TCCTGGTGCT CAAAGGTTTG AGAAGATGAT TGGAGGCCTT 
TACCTGGGTG AGCTGGTGAG GCTGGTTCTG GTCCACTTGA CCCAGCACGG GGTCCTCTTT 
GATGGCTGCG CCTCTCCTGC GTTGCTGAGT CAAGGCTGTA TCCTCCTGGA CCACGTGGCT 
GAGATGGAGG ACACCGCCAC TGGGACAGCC CGCGTCCACA CCATCCTGCA GGACTTGGGT 
CTGAGCCCTC GGGCCTCAGA TGCTGAACTG GTGCAATATG TGTGCGTGGC TGTGTGCACA 
CGGGCTGCCC AGCTCTGTGC GGCTGCCCTG GCTGCAGTCC TCTCCCGCCT CCAGCACAGC 
AGGGAGCAGC AGACATTGCA AGTGGCTGTG GCCACTGGAG GGCGAGTGTT TGAACGACAC 
CCCAGGTTCC TCCGAATCCT AAAGGAGACG GTAACACTCC TGGCCCCAAA CTGTGATGTC 
TCCTTCATCC CCTCTGTGGA TGGTGGTGGC CGGGGTGTGG CAATGGTGAC AGCTGTGGCT 
GCCCGCCTGG CTGCTCACAG GCGCATCCTG GAAGAGACCC TGGCACCATT TCAGCTGACC 
TTGGAGCAGA TGACAGTGGT GCAGGCACAA ATGCGGGAAG CCATGATCAG GGGGCTTCAA 
GGAGAGGCCT CCTCCCTCCG CATGCTGCCC ACTTACGTCC GAGCGACGCC CGACGGCAGC 
GAGCGAGGTG ATTTCCTGGC CTTGGACCTA GGGGGCACCA ACTTCCGGGT CCTGTTGGTA 
CGAGTGGCGG AGGGCAGTGT GCAGATCATC AACCAGGTCT ACTCCATTCC TGAGTGTAGA 
GCCCAGGGCT CTGGACAGAA GCTCTTTGAC CATATTGTGG ACTGCATCGT GGACTTCCAG 
AAGAGGCAGG GCCTTAGTGG ACAGAGCCTA CCCCTGGGTT TCACCTTCTC TTTCCCATGC 
AAGCAGCTTG GCCTGGACCA GGGCATCCTC CTGAATTGGA CTAAGGGGTT CAATGCATCA 
GGCTGTGAGG GCCAGGATGT TGTGTATCTA TTACGGGAAG CCATTAGGCG CAGACAGGCA 
GTGGAGCTGA ATGTGGTTGC CATTGTCAAT GACACGGTGG GGACCATGAT GTCCTGTGGC 
TATGATGATC CCCGTTGTGA GATGGGCCTC ATTGTCGGAA CCGGCACCAA CGCCTGTTAC 
ATGGAAGAGC TCCGCAATGT GGCAAGTGTG CCTGGGGACT CGGGCCTCAT GTGCATCAAC 
ATGGAGTGGG GTGCCTTTGG GGATGATGGC TCACTGGGCA CACTCAGCAC CCGCTTTGAC 
ACCAGTGTGG ACCAGGCATC CATCAATCCA GGCAAACAGA GGTTTGAGAA AATGATCAGC 
GGTATGTACT TGGGGGAGAT CGTCCGCCAT ATCCTCCTGC ACTTAACCAA TCTCGGAGTT 
CTCTTCCGAG GCCAGAAGAC TCAATGCCTT CAGGCCAGGG ACATCTTCAA GACTAAGTTC 
CTCTCTGAGA TCGAAAGTGA TAGCCTGGCC CTGCGTCAGG TCCGAGCCAT CCTGGAAGAT 
CTGGGGCTGA CTCTGACATC TGATGACGCC TTGATGGTCC TGGAAGTGTG CCAGGCTGTG 
TCTCGCAGGG CTGCCCAACT CTGTGGGGCA GGTGTGGCTG CCGTGGTGGA GAAGATTCGG 
GAGAACCGGG GCCTGCAGGA GCTGACAGTG TCTGTGGGAG TAGATGGGAC ACTCTACAAG 
CTACATCCTC ACTTCTCCAA GCTGGTGTCG GCAACAGTCC GGAAGCTAGC CCCTCAGTGC 
ACCGTCACCT TTTTGCAGTC GGAGGATGGG TCTGGCAAAG GCGCAGCTTT GGTTACTGCT 
GTTGCTTGTC GCCTTACCCA GATGGCCCAC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Developmental cell40(4)331-341.e4(2017 02)
Bulusu V,Prior N,Snaebjornsson MT,Kuehne A,Sonnen KF,Kress J,Stein F,Schultz C,Sauer U,Aulehla A

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Molecular and cellular biology36(15)2089-104(2016 Aug)
Bowman CE,Zhao L,Hartung T,Wolfgang MJ

Chronic Activation of ?2 AMPK Induces Obesity and Reduces ? Cell Function.
Cell metabolism23(5)821-36(2016 May)
Yavari A,Stocker CJ,Ghaffari S,Wargent ET,Steeples V,Czibik G,Pinter K,Bellahcene M,Woods A,Mart?nez de Morentin PB,Cansell C,Lam BY,Chuster A,Petkevicius K,Nguyen-Tu MS,Martinez-Sanchez A,Pullen TJ,Oliver PL,Stockenhuber A,Nguyen C,Lazdam M,O'Dowd JF,Harikumar P,T?th M,Beall C,Kyriakou T,Parnis J,Sarma D,Katritsis G,Wortmann DD,Harper AR,Brown LA,Willows R,Gandra S,Poncio V,de Oliveira Figueiredo MJ,Qi NR,Peirson SN,McCrimmon RJ,Gereben B,Tretter L,Fekete C,Redwood C,Yeo GS,Heisler LK,Rutter GA,Smith MA,Withers DJ,Carling D,Sternick EB,Arch JR,Cawthorne MA,Watkins H,Ashrafian H

Direct neuronal glucose uptake heralds activity-dependent increases in cerebral metabolism.
Nature communications66807(2015 Apr)
Lundgaard I,Li B,Xie L,Kang H,Sanggaard S,Haswell JD,Sun W,Goldman S,Blekot S,Nielsen M,Takano T,Deane R,Nedergaard M

Lactate downregulates the glycolytic enzymes hexokinase and phosphofructokinase in diverse tissues from mice.
FEBS letters585(1)92-8(2011 Jan)
Leite TC,Coelho RG,Da Silva D,Coelho WS,Marinho-Carvalho MM,Sola-Penna M

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Hk3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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