HK3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HK3
Entrez Gene ID	3101
Full Name	hexokinase 3
Synonyms	HKIII,HXK3
General protein information	Preferred Names hexokinase 3 Names hexokinase-3 ATP:D-hexose 6-phosphotransferase HK III hexokinase 3 (white cell) hexokinase type III
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q35.2
Summary	Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. [provided by RefSeq, Apr 2009].
Disorder MIM:	142570

mRNA and Protein(s)

mRNA	Protein	Name
NM_002115.3	NP_002106.2	hexokinase-3
XM_011534540.2	XP_011532842.1	hexokinase-3 isoform X2
NM_002115.2	NP_002106.2	hexokinase-3
XM_017009411.1	XP_016864900.1	hexokinase-3 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HK3	XP_518124.2
	HK3	XP_001086179.1
Mus musculus (house mouse)	Hk3	NP_001028417.1
Gallus gallus (chicken)	HK3	XP_001231329.3
Homo sapiens (human)	HK3	NP_002106.2
Canis lupus familiaris (dog)	HK3	XP_546212.4
Rattus norvegicus (Norway rat)	Hk3	NP_071515.1

Gene Ontology
Bibliography

Related articles in PubMed

Human gingiva transcriptome during wound healing.
Wang Y, Tatakis DN
Journal of clinical periodontology44(4)394-402(2017 Apr)

High 18F-FDG uptake in PMAH correlated with normal expression of Glut1, HK1, HK2, and HK3.
Cavalcante IP, Zerbini MC, Alencar GA, Mariani Bde P, Buchpiguel CA, Almeida MQ, Mendonca BB, Fragoso MC
Acta radiologica (Stockholm, Sweden : 1987)57(3)370-7(2016 Mar)

[Evaluation of Gene Expression of Hexokinases in Colorectal Cancer with the Use of Bioinformatics Methods].
Krasnov GS, Dmitriev AA, Sadtritdinova AF, Fedorova MS, Snezhkina AV, Melnikova NV, Poteryakhina AV, Nyushko KM, Belyakov MM, Kaprin AD, Zaretsky AR, Kudryavtseva AV
Biofizika60(6)1050-6(2015 Nov-Dec)

Identification of key genes affecting disease free survival time of pediatric acute lymphoblastic leukemia based on bioinformatic analysis.
Gao HY, Luo XG, Chen X, Wang JH
Blood cells, molecules & diseases54(1)38-43(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HK3 expression is significantly upregulated in human masticatory mucosa during wound healing
Title: Human gingiva transcriptome during wound healing.

The transport of glucose across the cell membrane by glucose transporters (GLUTs) and intracellular phosphorylation by hexokinases (HKs) are the initial steps of the glycolytic pathway.
Title: High 18F-FDG uptake in PMAH correlated with normal expression of Glut1, HK1, HK2, and HK3.

HK1 and HK2 expression alterations were detected, that could be explained by common deregulation mechanisms of these genes in colorectal tumors. The HK3 expression level was significantly increased in 60% of samples.
Title: [Evaluation of Gene Expression of Hexokinases in Colorectal Cancer with the Use of Bioinformatics Methods].

Through glycolysis and arachidonic acid metabolism, HK3 and PTGS2 might play important roles in pediatric ALL and its prognosis, and thus, might be potential targets for therapeutic intervention to suppress pediatric ALL.
Title: Identification of key genes affecting disease free survival time of pediatric acute lymphoblastic leukemia based on bioinformatic analysis.

Altogether, our data provide an explanation for low HK3 and KLF5 expression in particular AML subtype and establish these genes as novel CEBPA targets during neutrophil differentiation.
Title: CEBPA-dependent HK3 and KLF5 expression in primary AML and during AML differentiation.

The following HK3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HK3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09098	NM_002115.3 Latest version!	Homo sapiens hexokinase 3 (HK3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu58220	XM_011534540.2 Latest version!	Homo sapiens hexokinase 3 (HK3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu95852	XM_017009411.1 Latest version!	Homo sapiens hexokinase 3 (HK3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OHu09098	NM_002115.2	Homo sapiens hexokinase 3 (HK3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HK3 ( NM_002115.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002106.2 Homo sapiens hexokinase 3 (HK3), mRNA.

Price & Availability↑

CloneID	OHu09098
Clone ID Related Accession (Same CDS sequence)	NM_002115.3 , NM_002115.2
Accession Version	NM_002115.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	hexokinase-3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC027318.5, BC028129.1, BX644742.1 and U51333.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_002115.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U51333.1, BC028129.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000292432.10/ ENSP00000292432.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACTCCA TTGGGTCTTC AGGGTTGCGG CAGGGGGAAG AAACCCTGAG TTGCTCTGAG 
GAGGGCTTGC CCGGGCCCTC AGACAGCTCA GAGCTGGTGC AGGAGTGCCT GCAGCAGTTC 
AAGGTGACAA GGGCACAGCT ACAGCAGATC CAAGCCAGCC TCTTGGGTTC CATGGAGCAG 
GCGCTGAGGG GACAGGCCAG CCCTGCCCCT GCGGTCCGGA TGCTGCCTAC ATACGTGGGG 
TCCACCCCAC ATGGCACTGA GCAAGGAGAC TTCGTGGTGC TGGAGCTGGG GGCCACAGGG 
GCCTCACTGC GTGTTTTGTG GGTGACTCTA ACTGGCATTG AGGGGCATAG GGTGGAGCCC 
AGAAGCCAGG AGTTTGTGAT CCCCCAAGAG GTGATGCTGG GTGCTGGCCA GCAGCTCTTT 
GACTTTGCTG CCCACTGCCT GTCTGAGTTC CTGGATGCGC AGCCTGTGAA CAAACAGGGT 
CTGCAGCTTG GCTTCAGCTT CTCTTTCCCT TGTCACCAGA CGGGCTTGGA CAGGAGCACC 
CTCATTTCCT GGACCAAAGG TTTTAGGTGC AGTGGTGTGG AAGGCCAGGA TGTGGTCCAG 
CTGCTGAGAG ATGCCATTCG GAGGCAGGGG GCCTACAACA TCGACGTGGT TGCTGTGGTG 
AACGACACAG TGGGCACCAT GATGGGCTGT GAGCCGGGGG TCAGGCCGTG TGAGGTTGGG 
CTAGTTGTAG ACACGGGCAC CAACGCGTGT TACATGGAGG AGGCACGGCA TGTGGCAGTG 
CTGGACGAAG ACCGGGGCCG CGTCTGCGTC AGCGTCGAGT GGGGCTCCTT CAGCGATGAT 
GGGGCGCTGG GACCAGTGCT GACCACCTTC GACCATACCC TGGACCATGA GTCCCTGAAT 
CCTGGTGCTC AGAGGTTTGA GAAGATGATC GGAGGCCTGT ACCTGGGTGA GCTGGTGCGG 
CTGGTGCTGG CTCACTTGGC CCGGTGTGGG GTCCTCTTTG GTGGCTGCAC CTCCCCTGCC 
CTGCTGAGCC AAGGCAGCAT CCTCCTGGAA CACGTGGCTG AGATGGAGGA CCCCTCTACT 
GGGGCAGCCC GTGTCCATGC TATCCTGCAG GACTTGGGCC TGAGCCCTGG GGCTTCGGAT 
GTTGAGCTTG TGCAGCACGT CTGTGCGGCC GTGTGCACGC GGGCTGCCCA GCTCTGTGCT 
GCCGCCCTGG CCGCTGTTCT CTCCTGCCTC CAGCACAGCC GGGAGCAACA AACACTCCAG 
GTTGCTGTGG CCACCGGAGG CCGAGTGTGT GAGCGGCACC CCAGGTTCTG CAGCGTCCTG 
CAGGGGACAG TGATGCTCCT GGCCCCGGAA TGCGATGTCT CCTTAATCCC CTCTGTGGAT 
GGTGGTGGCC GGGGAGTGGC GATGGTGACT GCCGTGGCTG CCCGTCTGGC TGCCCACCGG 
CGCCTGCTGG AGGAGACCCT GGCCCCATTC CGGTTGAACC ATGATCAACT GGCTGCGGTT 
CAGGCACAGA TGCGGAAGGC CATGGCCAAG GGGCTCCGAG GGGAGGCCTC CTCCCTTCGC 
ATGCTGCCCA CTTTCGTCCG GGCCACCCCT GACGGCAGCG AGCGAGGGGA TTTCCTGGCC 
CTGGACCTCG GGGGCACGAA CTTCCGTGTC CTCCTGGTAC GTGTGACCAC AGGCGTGCAG 
ATCACCAGCG AGATCTACTC CATTCCCGAG ACTGTGGCCC AGGGTTCTGG GCAGCAGCTC 
TTTGACCACA TCGTGGACTG CATCGTGGAC TTCCAGCAGA AGCAGGGCCT GAGCGGGCAG 
AGCCTCCCAC TGGGTTTTAC CTTCTCCTTC CCATGTAGGC AGCTTGGCCT AGACCAGGGC 
ATCCTCCTGA ACTGGACCAA GGGTTTCAAG GCATCAGACT GCGAGGGCCA AGATGTCGTG 
AGTCTGTTGC GGGAAGCCAT CACTCGCAGA CAGGCAGTGG AGCTGAATGT GGTTGCCATT 
GTCAATGACA CGGTGGGGAC CATGATGTCC TGTGGCTATG AGGACCCCCG TTGCGAGATA 
GGCCTCATTG TCGGAACCGG CACCAATGCC TGCTACATGG AGGAGCTCCG GAATGTGGCG 
GGCGTGCCTG GGGACTCAGG CCGCATGTGC ATCAACATGG AGTGGGGCGC CTTTGGGGAC 
GATGGCTCTC TGGCCATGCT CAGCACCCGC TTTGATGCAA GTGTGGACCA GGCGTCCATC 
AACCCCGGCA AGCAGAGGTT TGAAAAGATG ATCAGCGGCA TGTACCTGGG GGAGATCGTC 
CGCCACATCC TTTTACATTT AACCAGCCTT GGCGTTCTCT TCCGGGGCCA GCAGATCCAG 
CGCCTTCAGA CCAGGGACAT CTTCAAGACC AAGTTCCTCT CTGAGATCGA AAGTGACAGC 
CTGGCCCTGC GGCAGGTCCG AGCCATCCTA GAGGATCTGG GGCTACCCCT GACCTCAGAT 
GACGCCCTGA TGGTGCTAGA GGTGTGCCAG GCTGTGTCCC AGAGGGCTGC CCAGCTCTGT 
GGGGCGGGTG TAGCTGCCGT GGTGGAGAAG ATCCGGGAGA ACCGGGGCCT GGAAGAGCTG 
GCAGTGTCTG TGGGGGTGGA TGGAACGCTC TACAAGCTGC ACCCGCGCTT CTCCAGCCTG 
GTGGCGGCCA CAGTGCGGGA GCTGGCCCCT CGCTGTGTGG TCACGTTCCT GCAGTCAGAG 
GATGGGTCCG GCAAAGGTGC GGCCCTGGTC ACCGCTGTTG CCTGCCGCCT TGCGCAGTTG 
ACTCGTGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002106.2
CDS	107..2878
Translation	MDSIGSSGLRQGEETLSCSEEGLPGPSDSSELVQECLQQFKVTRAQLQQIQASLLGSMEQALRGQASPAPAVRMLPTYVGSTPHGTEQGDFVVLELGATGASLRVLWVTLTGIEGHRVEPRSQEFVIPQEVMLGAGQQLFDFAAHCLSEFLDAQPVNKQGLQLGFSFSFPCHQTGLDRSTLISWTKGFRCSGVEGQDVVQLLRDAIRRQGAYNIDVVAVVNDTVGTMMGCEPGVRPCEVGLVVDTGTNACYMEEARHVAVLDEDRGRVCVSVEWGSFSDDGALGPVLTTFDHTLDHESLNPGAQRFEKMIGGLYLGELVRLVLAHLARCGVLFGGCTSPALLSQGSILLEHVAEMEDPSTGAARVHAILQDLGLSPGASDVELVQHVCAAVCTRAAQLCAAALAAVLSCLQHSREQQTLQVAVATGGRVCERHPRFCSVLQGTVMLLAPECDVSLIPSVDGGGRGVAMVTAVAARLAAHRRLLEETLAPFRLNHDQLAAVQAQMRKAMAKGLRGEASSLRMLPTFVRATPDGSERGDFLALDLGGTNFRVLLVRVTTGVQITSEIYSIPETVAQGSGQQLFDHIVDCIVDFQQKQGLSGQSLPLGFTFSFPCRQLGLDQGILLNWTKGFKASDCEGQDVVSLLREAITRRQAVELNVVAIVNDTVGTMMSCGYEDPRCEIGLIVGTGTNACYMEELRNVAGVPGDSGRMCINMEWGAFGDDGSLAMLSTRFDASVDQASINPGKQRFEKMISGMYLGEIVRHILLHLTSLGVLFRGQQIQRLQTRDIFKTKFLSEIESDSLALRQVRAILEDLGLPLTSDDALMVLEVCQAVSQRAAQLCGAGVAAVVEKIRENRGLEELAVSVGVDGTLYKLHPRFSSLVAATVRELAPRCVVTFLQSEDGSGKGAALVTAVACRLAQLTRV

Target ORF information:

RefSeq Version	NM_002115.3
Organism	Homo sapiens(human)
Definition	Homo sapiens hexokinase 3 (HK3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002115.3

ORF Insert Sequence:

ATGGACTCCA TTGGGTCTTC AGGGTTGCGG CAGGGGGAAG AAACCCTGAG TTGCTCTGAG 
GAGGGCTTGC CCGGGCCCTC AGACAGCTCA GAGCTGGTGC AGGAGTGCCT GCAGCAGTTC 
AAGGTGACAA GGGCACAGCT ACAGCAGATC CAAGCCAGCC TCTTGGGTTC CATGGAGCAG 
GCGCTGAGGG GACAGGCCAG CCCTGCCCCT GCGGTCCGGA TGCTGCCTAC ATACGTGGGG 
TCCACCCCAC ATGGCACTGA GCAAGGAGAC TTCGTGGTGC TGGAGCTGGG GGCCACAGGG 
GCCTCACTGC GTGTTTTGTG GGTGACTCTA ACTGGCATTG AGGGGCATAG GGTGGAGCCC 
AGAAGCCAGG AGTTTGTGAT CCCCCAAGAG GTGATGCTGG GTGCTGGCCA GCAGCTCTTT 
GACTTTGCTG CCCACTGCCT GTCTGAGTTC CTGGATGCGC AGCCTGTGAA CAAACAGGGT 
CTGCAGCTTG GCTTCAGCTT CTCTTTCCCT TGTCACCAGA CGGGCTTGGA CAGGAGCACC 
CTCATTTCCT GGACCAAAGG TTTTAGGTGC AGTGGTGTGG AAGGCCAGGA TGTGGTCCAG 
CTGCTGAGAG ATGCCATTCG GAGGCAGGGG GCCTACAACA TCGACGTGGT TGCTGTGGTG 
AACGACACAG TGGGCACCAT GATGGGCTGT GAGCCGGGGG TCAGGCCGTG TGAGGTTGGG 
CTAGTTGTAG ACACGGGCAC CAACGCGTGT TACATGGAGG AGGCACGGCA TGTGGCAGTG 
CTGGACGAAG ACCGGGGCCG CGTCTGCGTC AGCGTCGAGT GGGGCTCCTT CAGCGATGAT 
GGGGCGCTGG GACCAGTGCT GACCACCTTC GACCATACCC TGGACCATGA GTCCCTGAAT 
CCTGGTGCTC AGAGGTTTGA GAAGATGATC GGAGGCCTGT ACCTGGGTGA GCTGGTGCGG 
CTGGTGCTGG CTCACTTGGC CCGGTGTGGG GTCCTCTTTG GTGGCTGCAC CTCCCCTGCC 
CTGCTGAGCC AAGGCAGCAT CCTCCTGGAA CACGTGGCTG AGATGGAGGA CCCCTCTACT 
GGGGCAGCCC GTGTCCATGC TATCCTGCAG GACTTGGGCC TGAGCCCTGG GGCTTCGGAT 
GTTGAGCTTG TGCAGCACGT CTGTGCGGCC GTGTGCACGC GGGCTGCCCA GCTCTGTGCT 
GCCGCCCTGG CCGCTGTTCT CTCCTGCCTC CAGCACAGCC GGGAGCAACA AACACTCCAG 
GTTGCTGTGG CCACCGGAGG CCGAGTGTGT GAGCGGCACC CCAGGTTCTG CAGCGTCCTG 
CAGGGGACAG TGATGCTCCT GGCCCCGGAA TGCGATGTCT CCTTAATCCC CTCTGTGGAT 
GGTGGTGGCC GGGGAGTGGC GATGGTGACT GCCGTGGCTG CCCGTCTGGC TGCCCACCGG 
CGCCTGCTGG AGGAGACCCT GGCCCCATTC CGGTTGAACC ATGATCAACT GGCTGCGGTT 
CAGGCACAGA TGCGGAAGGC CATGGCCAAG GGGCTCCGAG GGGAGGCCTC CTCCCTTCGC 
ATGCTGCCCA CTTTCGTCCG GGCCACCCCT GACGGCAGCG AGCGAGGGGA TTTCCTGGCC 
CTGGACCTCG GGGGCACGAA CTTCCGTGTC CTCCTGGTAC GTGTGACCAC AGGCGTGCAG 
ATCACCAGCG AGATCTACTC CATTCCCGAG ACTGTGGCCC AGGGTTCTGG GCAGCAGCTC 
TTTGACCACA TCGTGGACTG CATCGTGGAC TTCCAGCAGA AGCAGGGCCT GAGCGGGCAG 
AGCCTCCCAC TGGGTTTTAC CTTCTCCTTC CCATGTAGGC AGCTTGGCCT AGACCAGGGC 
ATCCTCCTGA ACTGGACCAA GGGTTTCAAG GCATCAGACT GCGAGGGCCA AGATGTCGTG 
AGTCTGTTGC GGGAAGCCAT CACTCGCAGA CAGGCAGTGG AGCTGAATGT GGTTGCCATT 
GTCAATGACA CGGTGGGGAC CATGATGTCC TGTGGCTATG AGGACCCCCG TTGCGAGATA 
GGCCTCATTG TCGGAACCGG CACCAATGCC TGCTACATGG AGGAGCTCCG GAATGTGGCG 
GGCGTGCCTG GGGACTCAGG CCGCATGTGC ATCAACATGG AGTGGGGCGC CTTTGGGGAC 
GATGGCTCTC TGGCCATGCT CAGCACCCGC TTTGATGCAA GTGTGGACCA GGCGTCCATC 
AACCCCGGCA AGCAGAGGTT TGAAAAGATG ATCAGCGGCA TGTACCTGGG GGAGATCGTC 
CGCCACATCC TTTTACATTT AACCAGCCTT GGCGTTCTCT TCCGGGGCCA GCAGATCCAG 
CGCCTTCAGA CCAGGGACAT CTTCAAGACC AAGTTCCTCT CTGAGATCGA AAGTGACAGC 
CTGGCCCTGC GGCAGGTCCG AGCCATCCTA GAGGATCTGG GGCTACCCCT GACCTCAGAT 
GACGCCCTGA TGGTGCTAGA GGTGTGCCAG GCTGTGTCCC AGAGGGCTGC CCAGCTCTGT 
GGGGCGGGTG TAGCTGCCGT GGTGGAGAAG ATCCGGGAGA ACCGGGGCCT GGAAGAGCTG 
GCAGTGTCTG TGGGGGTGGA TGGAACGCTC TACAAGCTGC ACCCGCGCTT CTCCAGCCTG 
GTGGCGGCCA CAGTGCGGGA GCTGGCCCCT CGCTGTGTGG TCACGTTCCT GCAGTCAGAG 
GATGGGTCCG GCAAAGGTGC GGCCCTGGTC ACCGCTGTTG CCTGCCGCCT TGCGCAGTTG 
ACTCGTGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HK3 ( XM_011534540.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532842.1 Homo sapiens hexokinase 3 (HK3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu58220
Clone ID Related Accession (Same CDS sequence)	XM_011534540.2
Accession Version	XM_011534540.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	hexokinase-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534540.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCA TTGGGTCTTC AGGGTTGCGG CAGGGGGAAG AAACCCTGAG TTGCTCTGAG 
GAGGGCTTGC CCGGGCCCTC AGACAGCTCA GAGCTGGTGC AGGAGTGCCT GCAGCAGTTC 
AAGGTGACAA GGGCACAGCT ACAGCAGATC CAAGCCAGCC TCTTGGGTTC CATGGAGCAG 
GCGCTGAGGG GACAGGCCAG CCCTGCCCCT GCGGTCCGGA TGCTGCCTAC ATACGTGGGG 
TCCACCCCAC ATGGCACTGA GCAAGGAGAC TTCGTGGTGC TGGAGCTGGG GGCCACAGGG 
GCCTCACTGC GTGTTTTGTG GGTGACTCTA ACTGGCATTG AGGGGCATAG GGTGGAGCCC 
AGAAGCCAGG AGTTTGTGAT CCCCCAAGAG GTGATGCTGG GTGCTGGCCA GCAGCTCTTT 
GACTTTGCTG CCCACTGCCT GTCTGAGTTC CTGGATGCGC AGCCTGTGAA CAAACAGGGT 
CTGCAGCTTG GCTTCAGCTT CTCTTTCCCT TGTCACCAGA CGGGCTTGGA CAGGAGCACC 
CTCATTTCCT GGACCAAAGG TTTTAGGTGC AGTGGTGTGG AAGGCCAGGA TGTGGTCCAG 
CTGCTGAGAG ATGCCATTCG GAGGCAGGGG GCCTACAACA TCGACGTGGT TGCTGTGGTG 
AACGACACAG TGGGCACCAT GATGGGCTGT GAGCCGGGGG TCAGGCCGTG TGAGGTTGGG 
CTAGTTGTAG ACACGGGCAC CAACGCGTGT TACATGGAGG AGGCACGGCA TGTGGCAGTG 
CTGGACGAAG ACCGGGGCCG CGTCTGCGTC AGCGTCGAGT GGGGCTCCTT CAGCGATGAT 
GGGGCGCTGG GACCAGTGCT GACCACCTTC GACCATACCC TGGACCATGA GTCCCTGAAT 
CCTGGTGCTC AGAGGTTTGA GAAGATGATC GGAGGCCTGT ACCTGGGTGA GCTGGTGCGG 
CTGGTGCTGG CTCACTTGGC CCGGTGTGGG GTCCTCTTTG GTGGCTGCAC CTCCCCTGCC 
CTGCTGAGCC AAGGCAGCAT CCTCCTGGAA CACGTGGCTG AGATGGAGGA CCCCTCTACT 
GGGGCAGCCC GTGTCCATGC TATCCTGCAG GACTTGGGCC TGAGCCCTGG GGCTTCGGAT 
GTTGAGCTTG TGCAGCACGT CTGTGCGGCC GTGTGCACGC GGGCTGCCCA GCTCTGTGCT 
GCCGCCCTGG CCGCTGTTCT CTCCTGCCTC CAGCACAGCC GGGAGCAACA AACACTCCAG 
GTTGCTGTGG CCACCGGAGG CCGAGTGTGT GAGCGGCACC CCAGGTTCTG CAGCGTCCTG 
CAGGGGACAG TGATGCTCCT GGCCCCGGAA TGCGATGTCT CCTTAATCCC CTCTGTGGAT 
GGTGGTGGCC GGGGAGTGGC GATGGTGACT GCCGTGGCTG CCCGTCTGGC TGCCCACCGG 
CGCCTGCTGG AGGAGACCCT GGCCCCATTC CGGTTGAACC ATGATCAACT GGCTGCGGTT 
CAGGCACAGA TGCGGAAGGC CATGGCCAAG GGGCTCCGAG GGGAGGCCTC CTCCCTTCGC 
ATGCTGCCCA CTTTCGTCCG GGCCACCCCT GACGGCAGCG AGCGAGGGGA TTTCCTGGCC 
CTGGACCTCG GGGGCACGAA CTTCCGTGTC CTCCTGGTAC GTGTGACCAC AGGCGTGCAG 
ATCACCAGCG AGATCTACTC CATTCCCGAG ACTGTGGCCC AGGGTTCTGG GCAGCAGCTC 
TTTGACCACA TCGTGGACTG CATCGTGGAC TTCCAGCAGA AGCAGGGCCT GAGCGGGCAG 
AGCCTCCCAC TGGGTTTTAC CTTCTCCTTC CCATGTAGGC AGCTTGGCCT AGACCAGTGG 
GCCAGTCTTG CTGCTTGGCT GCTCTGCTCC AGGCTAAGCC TGCTCAGTCC TTTTGATTAT 
TCCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532842.1
CDS	134..2062
Translation	MDSIGSSGLRQGEETLSCSEEGLPGPSDSSELVQECLQQFKVTRAQLQQIQASLLGSMEQALRGQASPAPAVRMLPTYVGSTPHGTEQGDFVVLELGATGASLRVLWVTLTGIEGHRVEPRSQEFVIPQEVMLGAGQQLFDFAAHCLSEFLDAQPVNKQGLQLGFSFSFPCHQTGLDRSTLISWTKGFRCSGVEGQDVVQLLRDAIRRQGAYNIDVVAVVNDTVGTMMGCEPGVRPCEVGLVVDTGTNACYMEEARHVAVLDEDRGRVCVSVEWGSFSDDGALGPVLTTFDHTLDHESLNPGAQRFEKMIGGLYLGELVRLVLAHLARCGVLFGGCTSPALLSQGSILLEHVAEMEDPSTGAARVHAILQDLGLSPGASDVELVQHVCAAVCTRAAQLCAAALAAVLSCLQHSREQQTLQVAVATGGRVCERHPRFCSVLQGTVMLLAPECDVSLIPSVDGGGRGVAMVTAVAARLAAHRRLLEETLAPFRLNHDQLAAVQAQMRKAMAKGLRGEASSLRMLPTFVRATPDGSERGDFLALDLGGTNFRVLLVRVTTGVQITSEIYSIPETVAQGSGQQLFDHIVDCIVDFQQKQGLSGQSLPLGFTFSFPCRQLGLDQWASLAAWLLCSRLSLLSPFDYSL

Target ORF information:

RefSeq Version	XM_011534540.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hexokinase 3 (HK3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534540.2

ORF Insert Sequence:

ATGGACTCCA TTGGGTCTTC AGGGTTGCGG CAGGGGGAAG AAACCCTGAG TTGCTCTGAG 
GAGGGCTTGC CCGGGCCCTC AGACAGCTCA GAGCTGGTGC AGGAGTGCCT GCAGCAGTTC 
AAGGTGACAA GGGCACAGCT ACAGCAGATC CAAGCCAGCC TCTTGGGTTC CATGGAGCAG 
GCGCTGAGGG GACAGGCCAG CCCTGCCCCT GCGGTCCGGA TGCTGCCTAC ATACGTGGGG 
TCCACCCCAC ATGGCACTGA GCAAGGAGAC TTCGTGGTGC TGGAGCTGGG GGCCACAGGG 
GCCTCACTGC GTGTTTTGTG GGTGACTCTA ACTGGCATTG AGGGGCATAG GGTGGAGCCC 
AGAAGCCAGG AGTTTGTGAT CCCCCAAGAG GTGATGCTGG GTGCTGGCCA GCAGCTCTTT 
GACTTTGCTG CCCACTGCCT GTCTGAGTTC CTGGATGCGC AGCCTGTGAA CAAACAGGGT 
CTGCAGCTTG GCTTCAGCTT CTCTTTCCCT TGTCACCAGA CGGGCTTGGA CAGGAGCACC 
CTCATTTCCT GGACCAAAGG TTTTAGGTGC AGTGGTGTGG AAGGCCAGGA TGTGGTCCAG 
CTGCTGAGAG ATGCCATTCG GAGGCAGGGG GCCTACAACA TCGACGTGGT TGCTGTGGTG 
AACGACACAG TGGGCACCAT GATGGGCTGT GAGCCGGGGG TCAGGCCGTG TGAGGTTGGG 
CTAGTTGTAG ACACGGGCAC CAACGCGTGT TACATGGAGG AGGCACGGCA TGTGGCAGTG 
CTGGACGAAG ACCGGGGCCG CGTCTGCGTC AGCGTCGAGT GGGGCTCCTT CAGCGATGAT 
GGGGCGCTGG GACCAGTGCT GACCACCTTC GACCATACCC TGGACCATGA GTCCCTGAAT 
CCTGGTGCTC AGAGGTTTGA GAAGATGATC GGAGGCCTGT ACCTGGGTGA GCTGGTGCGG 
CTGGTGCTGG CTCACTTGGC CCGGTGTGGG GTCCTCTTTG GTGGCTGCAC CTCCCCTGCC 
CTGCTGAGCC AAGGCAGCAT CCTCCTGGAA CACGTGGCTG AGATGGAGGA CCCCTCTACT 
GGGGCAGCCC GTGTCCATGC TATCCTGCAG GACTTGGGCC TGAGCCCTGG GGCTTCGGAT 
GTTGAGCTTG TGCAGCACGT CTGTGCGGCC GTGTGCACGC GGGCTGCCCA GCTCTGTGCT 
GCCGCCCTGG CCGCTGTTCT CTCCTGCCTC CAGCACAGCC GGGAGCAACA AACACTCCAG 
GTTGCTGTGG CCACCGGAGG CCGAGTGTGT GAGCGGCACC CCAGGTTCTG CAGCGTCCTG 
CAGGGGACAG TGATGCTCCT GGCCCCGGAA TGCGATGTCT CCTTAATCCC CTCTGTGGAT 
GGTGGTGGCC GGGGAGTGGC GATGGTGACT GCCGTGGCTG CCCGTCTGGC TGCCCACCGG 
CGCCTGCTGG AGGAGACCCT GGCCCCATTC CGGTTGAACC ATGATCAACT GGCTGCGGTT 
CAGGCACAGA TGCGGAAGGC CATGGCCAAG GGGCTCCGAG GGGAGGCCTC CTCCCTTCGC 
ATGCTGCCCA CTTTCGTCCG GGCCACCCCT GACGGCAGCG AGCGAGGGGA TTTCCTGGCC 
CTGGACCTCG GGGGCACGAA CTTCCGTGTC CTCCTGGTAC GTGTGACCAC AGGCGTGCAG 
ATCACCAGCG AGATCTACTC CATTCCCGAG ACTGTGGCCC AGGGTTCTGG GCAGCAGCTC 
TTTGACCACA TCGTGGACTG CATCGTGGAC TTCCAGCAGA AGCAGGGCCT GAGCGGGCAG 
AGCCTCCCAC TGGGTTTTAC CTTCTCCTTC CCATGTAGGC AGCTTGGCCT AGACCAGTGG 
GCCAGTCTTG CTGCTTGGCT GCTCTGCTCC AGGCTAAGCC TGCTCAGTCC TTTTGATTAT 
TCCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HK3 ( NM_002115.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_002106.2 Homo sapiens hexokinase 3 (HK3), mRNA.

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CloneID	OHu09098
Clone ID Related Accession (Same CDS sequence)	NM_002115.3 , NM_002115.2
Accession Version	NM_002115.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	hexokinase-3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC028129.1, BX644742.1 and U51333.1. This sequence is a reference standard in the RefSeqGene project. On Jul 11, 2008 this sequence version replaced NM_002115.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028129.1, SRR1660809.86090.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGACTCCA TTGGGTCTTC AGGGTTGCGG CAGGGGGAAG AAACCCTGAG TTGCTCTGAG 
GAGGGCTTGC CCGGGCCCTC AGACAGCTCA GAGCTGGTGC AGGAGTGCCT GCAGCAGTTC 
AAGGTGACAA GGGCACAGCT ACAGCAGATC CAAGCCAGCC TCTTGGGTTC CATGGAGCAG 
GCGCTGAGGG GACAGGCCAG CCCTGCCCCT GCGGTCCGGA TGCTGCCTAC ATACGTGGGG 
TCCACCCCAC ATGGCACTGA GCAAGGAGAC TTCGTGGTGC TGGAGCTGGG GGCCACAGGG 
GCCTCACTGC GTGTTTTGTG GGTGACTCTA ACTGGCATTG AGGGGCATAG GGTGGAGCCC 
AGAAGCCAGG AGTTTGTGAT CCCCCAAGAG GTGATGCTGG GTGCTGGCCA GCAGCTCTTT 
GACTTTGCTG CCCACTGCCT GTCTGAGTTC CTGGATGCGC AGCCTGTGAA CAAACAGGGT 
CTGCAGCTTG GCTTCAGCTT CTCTTTCCCT TGTCACCAGA CGGGCTTGGA CAGGAGCACC 
CTCATTTCCT GGACCAAAGG TTTTAGGTGC AGTGGTGTGG AAGGCCAGGA TGTGGTCCAG 
CTGCTGAGAG ATGCCATTCG GAGGCAGGGG GCCTACAACA TCGACGTGGT TGCTGTGGTG 
AACGACACAG TGGGCACCAT GATGGGCTGT GAGCCGGGGG TCAGGCCGTG TGAGGTTGGG 
CTAGTTGTAG ACACGGGCAC CAACGCGTGT TACATGGAGG AGGCACGGCA TGTGGCAGTG 
CTGGACGAAG ACCGGGGCCG CGTCTGCGTC AGCGTCGAGT GGGGCTCCTT CAGCGATGAT 
GGGGCGCTGG GACCAGTGCT GACCACCTTC GACCATACCC TGGACCATGA GTCCCTGAAT 
CCTGGTGCTC AGAGGTTTGA GAAGATGATC GGAGGCCTGT ACCTGGGTGA GCTGGTGCGG 
CTGGTGCTGG CTCACTTGGC CCGGTGTGGG GTCCTCTTTG GTGGCTGCAC CTCCCCTGCC 
CTGCTGAGCC AAGGCAGCAT CCTCCTGGAA CACGTGGCTG AGATGGAGGA CCCCTCTACT 
GGGGCAGCCC GTGTCCATGC TATCCTGCAG GACTTGGGCC TGAGCCCTGG GGCTTCGGAT 
GTTGAGCTTG TGCAGCACGT CTGTGCGGCC GTGTGCACGC GGGCTGCCCA GCTCTGTGCT 
GCCGCCCTGG CCGCTGTTCT CTCCTGCCTC CAGCACAGCC GGGAGCAACA AACACTCCAG 
GTTGCTGTGG CCACCGGAGG CCGAGTGTGT GAGCGGCACC CCAGGTTCTG CAGCGTCCTG 
CAGGGGACAG TGATGCTCCT GGCCCCGGAA TGCGATGTCT CCTTAATCCC CTCTGTGGAT 
GGTGGTGGCC GGGGAGTGGC GATGGTGACT GCCGTGGCTG CCCGTCTGGC TGCCCACCGG 
CGCCTGCTGG AGGAGACCCT GGCCCCATTC CGGTTGAACC ATGATCAACT GGCTGCGGTT 
CAGGCACAGA TGCGGAAGGC CATGGCCAAG GGGCTCCGAG GGGAGGCCTC CTCCCTTCGC 
ATGCTGCCCA CTTTCGTCCG GGCCACCCCT GACGGCAGCG AGCGAGGGGA TTTCCTGGCC 
CTGGACCTCG GGGGCACGAA CTTCCGTGTC CTCCTGGTAC GTGTGACCAC AGGCGTGCAG 
ATCACCAGCG AGATCTACTC CATTCCCGAG ACTGTGGCCC AGGGTTCTGG GCAGCAGCTC 
TTTGACCACA TCGTGGACTG CATCGTGGAC TTCCAGCAGA AGCAGGGCCT GAGCGGGCAG 
AGCCTCCCAC TGGGTTTTAC CTTCTCCTTC CCATGTAGGC AGCTTGGCCT AGACCAGGGC 
ATCCTCCTGA ACTGGACCAA GGGTTTCAAG GCATCAGACT GCGAGGGCCA AGATGTCGTG 
AGTCTGTTGC GGGAAGCCAT CACTCGCAGA CAGGCAGTGG AGCTGAATGT GGTTGCCATT 
GTCAATGACA CGGTGGGGAC CATGATGTCC TGTGGCTATG AGGACCCCCG TTGCGAGATA 
GGCCTCATTG TCGGAACCGG CACCAATGCC TGCTACATGG AGGAGCTCCG GAATGTGGCG 
GGCGTGCCTG GGGACTCAGG CCGCATGTGC ATCAACATGG AGTGGGGCGC CTTTGGGGAC 
GATGGCTCTC TGGCCATGCT CAGCACCCGC TTTGATGCAA GTGTGGACCA GGCGTCCATC 
AACCCCGGCA AGCAGAGGTT TGAAAAGATG ATCAGCGGCA TGTACCTGGG GGAGATCGTC 
CGCCACATCC TTTTACATTT AACCAGCCTT GGCGTTCTCT TCCGGGGCCA GCAGATCCAG 
CGCCTTCAGA CCAGGGACAT CTTCAAGACC AAGTTCCTCT CTGAGATCGA AAGTGACAGC 
CTGGCCCTGC GGCAGGTCCG AGCCATCCTA GAGGATCTGG GGCTACCCCT GACCTCAGAT 
GACGCCCTGA TGGTGCTAGA GGTGTGCCAG GCTGTGTCCC AGAGGGCTGC CCAGCTCTGT 
GGGGCGGGTG TAGCTGCCGT GGTGGAGAAG ATCCGGGAGA ACCGGGGCCT GGAAGAGCTG 
GCAGTGTCTG TGGGGGTGGA TGGAACGCTC TACAAGCTGC ACCCGCGCTT CTCCAGCCTG 
GTGGCGGCCA CAGTGCGGGA GCTGGCCCCT CGCTGTGTGG TCACGTTCCT GCAGTCAGAG 
GATGGGTCCG GCAAAGGTGC GGCCCTGGTC ACCGCTGTTG CCTGCCGCCT TGCGCAGTTG 
ACTCGTGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002106.2
CDS	93..2864
Translation	MDSIGSSGLRQGEETLSCSEEGLPGPSDSSELVQECLQQFKVTRAQLQQIQASLLGSMEQALRGQASPAPAVRMLPTYVGSTPHGTEQGDFVVLELGATGASLRVLWVTLTGIEGHRVEPRSQEFVIPQEVMLGAGQQLFDFAAHCLSEFLDAQPVNKQGLQLGFSFSFPCHQTGLDRSTLISWTKGFRCSGVEGQDVVQLLRDAIRRQGAYNIDVVAVVNDTVGTMMGCEPGVRPCEVGLVVDTGTNACYMEEARHVAVLDEDRGRVCVSVEWGSFSDDGALGPVLTTFDHTLDHESLNPGAQRFEKMIGGLYLGELVRLVLAHLARCGVLFGGCTSPALLSQGSILLEHVAEMEDPSTGAARVHAILQDLGLSPGASDVELVQHVCAAVCTRAAQLCAAALAAVLSCLQHSREQQTLQVAVATGGRVCERHPRFCSVLQGTVMLLAPECDVSLIPSVDGGGRGVAMVTAVAARLAAHRRLLEETLAPFRLNHDQLAAVQAQMRKAMAKGLRGEASSLRMLPTFVRATPDGSERGDFLALDLGGTNFRVLLVRVTTGVQITSEIYSIPETVAQGSGQQLFDHIVDCIVDFQQKQGLSGQSLPLGFTFSFPCRQLGLDQGILLNWTKGFKASDCEGQDVVSLLREAITRRQAVELNVVAIVNDTVGTMMSCGYEDPRCEIGLIVGTGTNACYMEELRNVAGVPGDSGRMCINMEWGAFGDDGSLAMLSTRFDASVDQASINPGKQRFEKMISGMYLGEIVRHILLHLTSLGVLFRGQQIQRLQTRDIFKTKFLSEIESDSLALRQVRAILEDLGLPLTSDDALMVLEVCQAVSQRAAQLCGAGVAAVVEKIRENRGLEELAVSVGVDGTLYKLHPRFSSLVAATVRELAPRCVVTFLQSEDGSGKGAALVTAVACRLAQLTRV

Target ORF information:

RefSeq Version	NM_002115.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hexokinase 3 (HK3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002115.2

ORF Insert Sequence:

ATGGACTCCA TTGGGTCTTC AGGGTTGCGG CAGGGGGAAG AAACCCTGAG TTGCTCTGAG 
GAGGGCTTGC CCGGGCCCTC AGACAGCTCA GAGCTGGTGC AGGAGTGCCT GCAGCAGTTC 
AAGGTGACAA GGGCACAGCT ACAGCAGATC CAAGCCAGCC TCTTGGGTTC CATGGAGCAG 
GCGCTGAGGG GACAGGCCAG CCCTGCCCCT GCGGTCCGGA TGCTGCCTAC ATACGTGGGG 
TCCACCCCAC ATGGCACTGA GCAAGGAGAC TTCGTGGTGC TGGAGCTGGG GGCCACAGGG 
GCCTCACTGC GTGTTTTGTG GGTGACTCTA ACTGGCATTG AGGGGCATAG GGTGGAGCCC 
AGAAGCCAGG AGTTTGTGAT CCCCCAAGAG GTGATGCTGG GTGCTGGCCA GCAGCTCTTT 
GACTTTGCTG CCCACTGCCT GTCTGAGTTC CTGGATGCGC AGCCTGTGAA CAAACAGGGT 
CTGCAGCTTG GCTTCAGCTT CTCTTTCCCT TGTCACCAGA CGGGCTTGGA CAGGAGCACC 
CTCATTTCCT GGACCAAAGG TTTTAGGTGC AGTGGTGTGG AAGGCCAGGA TGTGGTCCAG 
CTGCTGAGAG ATGCCATTCG GAGGCAGGGG GCCTACAACA TCGACGTGGT TGCTGTGGTG 
AACGACACAG TGGGCACCAT GATGGGCTGT GAGCCGGGGG TCAGGCCGTG TGAGGTTGGG 
CTAGTTGTAG ACACGGGCAC CAACGCGTGT TACATGGAGG AGGCACGGCA TGTGGCAGTG 
CTGGACGAAG ACCGGGGCCG CGTCTGCGTC AGCGTCGAGT GGGGCTCCTT CAGCGATGAT 
GGGGCGCTGG GACCAGTGCT GACCACCTTC GACCATACCC TGGACCATGA GTCCCTGAAT 
CCTGGTGCTC AGAGGTTTGA GAAGATGATC GGAGGCCTGT ACCTGGGTGA GCTGGTGCGG 
CTGGTGCTGG CTCACTTGGC CCGGTGTGGG GTCCTCTTTG GTGGCTGCAC CTCCCCTGCC 
CTGCTGAGCC AAGGCAGCAT CCTCCTGGAA CACGTGGCTG AGATGGAGGA CCCCTCTACT 
GGGGCAGCCC GTGTCCATGC TATCCTGCAG GACTTGGGCC TGAGCCCTGG GGCTTCGGAT 
GTTGAGCTTG TGCAGCACGT CTGTGCGGCC GTGTGCACGC GGGCTGCCCA GCTCTGTGCT 
GCCGCCCTGG CCGCTGTTCT CTCCTGCCTC CAGCACAGCC GGGAGCAACA AACACTCCAG 
GTTGCTGTGG CCACCGGAGG CCGAGTGTGT GAGCGGCACC CCAGGTTCTG CAGCGTCCTG 
CAGGGGACAG TGATGCTCCT GGCCCCGGAA TGCGATGTCT CCTTAATCCC CTCTGTGGAT 
GGTGGTGGCC GGGGAGTGGC GATGGTGACT GCCGTGGCTG CCCGTCTGGC TGCCCACCGG 
CGCCTGCTGG AGGAGACCCT GGCCCCATTC CGGTTGAACC ATGATCAACT GGCTGCGGTT 
CAGGCACAGA TGCGGAAGGC CATGGCCAAG GGGCTCCGAG GGGAGGCCTC CTCCCTTCGC 
ATGCTGCCCA CTTTCGTCCG GGCCACCCCT GACGGCAGCG AGCGAGGGGA TTTCCTGGCC 
CTGGACCTCG GGGGCACGAA CTTCCGTGTC CTCCTGGTAC GTGTGACCAC AGGCGTGCAG 
ATCACCAGCG AGATCTACTC CATTCCCGAG ACTGTGGCCC AGGGTTCTGG GCAGCAGCTC 
TTTGACCACA TCGTGGACTG CATCGTGGAC TTCCAGCAGA AGCAGGGCCT GAGCGGGCAG 
AGCCTCCCAC TGGGTTTTAC CTTCTCCTTC CCATGTAGGC AGCTTGGCCT AGACCAGGGC 
ATCCTCCTGA ACTGGACCAA GGGTTTCAAG GCATCAGACT GCGAGGGCCA AGATGTCGTG 
AGTCTGTTGC GGGAAGCCAT CACTCGCAGA CAGGCAGTGG AGCTGAATGT GGTTGCCATT 
GTCAATGACA CGGTGGGGAC CATGATGTCC TGTGGCTATG AGGACCCCCG TTGCGAGATA 
GGCCTCATTG TCGGAACCGG CACCAATGCC TGCTACATGG AGGAGCTCCG GAATGTGGCG 
GGCGTGCCTG GGGACTCAGG CCGCATGTGC ATCAACATGG AGTGGGGCGC CTTTGGGGAC 
GATGGCTCTC TGGCCATGCT CAGCACCCGC TTTGATGCAA GTGTGGACCA GGCGTCCATC 
AACCCCGGCA AGCAGAGGTT TGAAAAGATG ATCAGCGGCA TGTACCTGGG GGAGATCGTC 
CGCCACATCC TTTTACATTT AACCAGCCTT GGCGTTCTCT TCCGGGGCCA GCAGATCCAG 
CGCCTTCAGA CCAGGGACAT CTTCAAGACC AAGTTCCTCT CTGAGATCGA AAGTGACAGC 
CTGGCCCTGC GGCAGGTCCG AGCCATCCTA GAGGATCTGG GGCTACCCCT GACCTCAGAT 
GACGCCCTGA TGGTGCTAGA GGTGTGCCAG GCTGTGTCCC AGAGGGCTGC CCAGCTCTGT 
GGGGCGGGTG TAGCTGCCGT GGTGGAGAAG ATCCGGGAGA ACCGGGGCCT GGAAGAGCTG 
GCAGTGTCTG TGGGGGTGGA TGGAACGCTC TACAAGCTGC ACCCGCGCTT CTCCAGCCTG 
GTGGCGGCCA CAGTGCGGGA GCTGGCCCCT CGCTGTGTGG TCACGTTCCT GCAGTCAGAG 
GATGGGTCCG GCAAAGGTGC GGCCCTGGTC ACCGCTGTTG CCTGCCGCCT TGCGCAGTTG 
ACTCGTGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HK3 ( XM_017009411.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864900.1 Homo sapiens hexokinase 3 (HK3), transcript variant X1, mRNA.

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CloneID	OHu95852
Clone ID Related Accession (Same CDS sequence)	XM_017009411.1
Accession Version	XM_017009411.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	hexokinase-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG CGCTGAGGGG ACAGGCCAGC CCTGCCCCTG CGGTCCGGAT GCTGCCTACA 
TACGTGGGGT CCACCCCACA TGGCACTGAG CAAGGAGACT TCGTGGTGCT GGAGCTGGGG 
GCCACAGGGG CCTCACTGCG TGTTTTGTGG GTGACTCTAA CTGGCATTGA GGGGCATAGG 
GTGGAGCCCA GAAGCCAGGA GTTTGTGATC CCCCAAGAGG TGATGCTGGG TGCTGGCCAG 
CAGCTCTTTG ACTTTGCTGC CCACTGCCTG TCTGAGTTCC TGGATGCGCA GCCTGTGAAC 
AAACAGGGTC TGCAGCTTGG CTTCAGCTTC TCTTTCCCTT GTCACCAGAC GGGCTTGGAC 
AGGAGCACCC TCATTTCCTG GACCAAAGGT TTTAGGTGCA GTGGTGTGGA AGGCCAGGAT 
GTGGTCCAGC TGCTGAGAGA TGCCATTCGG AGGCAGGGGG CCTACAACAT CGACGTGGTT 
GCTGTGGTGA ACGACACAGT GGGCACCATG ATGGGCTGTG AGCCGGGGGT CAGGCCGTGT 
GAGGTTGGGC TAGTTGTAGA CACGGGCACC AACGCGTGTT ACATGGAGGA GGCACGGCAT 
GTGGCAGTGC TGGACGAAGA CCGGGGCCGC GTCTGCGTCA GCGTCGAGTG GGGCTCCTTC 
AGCGATGATG GGGCGCTGGG ACCAGTGCTG ACCACCTTCG ACCATACCCT GGACCATGAG 
TCCCTGAATC CTGGTGCTCA GAGGTTTGAG AAGATGATCG GAGGCCTGTA CCTGGGTGAG 
CTGGTGCGGC TGGTGCTGGC TCACTTGGCC CGGTGTGGGG TCCTCTTTGG TGGCTGCACC 
TCCCCTGCCC TGCTGAGCCA AGGCAGCATC CTCCTGGAAC ACGTGGCTGA GATGGAGGAC 
CCCTCTACTG GGGCAGCCCG TGTCCATGCT ATCCTGCAGG ACTTGGGCCT GAGCCCTGGG 
GCTTCGGATG TTGAGCTTGT GCAGCACGTC TGTGCGGCCG TGTGCACGCG GGCTGCCCAG 
CTCTGTGCTG CCGCCCTGGC CGCTGTTCTC TCCTGCCTCC AGCACAGCCG GGAGCAACAA 
ACACTCCAGG TTGCTGTGGC CACCGGAGGC CGAGTGTGTG AGCGGCACCC CAGGTTCTGC 
AGCGTCCTGC AGGGGACAGT GATGCTCCTG GCCCCGGAAT GCGATGTCTC CTTAATCCCC 
TCTGTGGATG GTGGTGGCCG GGGAGTGGCG ATGGTGACTG CCGTGGCTGC CCGTCTGGCT 
GCCCACCGGC GCCTGCTGGA GGAGACCCTG GCCCCATTCC GGTTGAACCA TGATCAACTG 
GCTGCGGTTC AGGCACAGAT GCGGAAGGCC ATGGCCAAGG GGCTCCGAGG GGAGGCCTCC 
TCCCTTCGCA TGCTGCCCAC TTTCGTCCGG GCCACCCCTG ACGGCAGCGA GCGAGGGGAT 
TTCCTGGCCC TGGACCTCGG GGGCACGAAC TTCCGTGTCC TCCTGGTACG TGTGACCACA 
GGCGTGCAGA TCACCAGCGA GATCTACTCC ATTCCCGAGA CTGTGGCCCA GGGTTCTGGG 
CAGCAGCTCT TTGACCACAT CGTGGACTGC ATCGTGGACT TCCAGCAGAA GCAGGGCCTG 
AGCGGGCAGA GCCTCCCACT GGGTTTTACC TTCTCCTTCC CATGTAGGCA GCTTGGCCTA 
GACCAGGGCA TCCTCCTGAA CTGGACCAAG GGTTTCAAGG CATCAGACTG CGAGGGCCAA 
GATGTCGTGA GTCTGTTGCG GGAAGCCATC ACTCGCAGAC AGGCAGTGGA GCTGAATGTG 
GTTGCCATTG TCAATGACAC GGTGGGGACC ATGATGTCCT GTGGCTATGA GGACCCCCGT 
TGCGAGATAG GCCTCATTGT CGGAACCGGC ACCAATGCCT GCTACATGGA GGAGCTCCGG 
AATGTGGCGG GCGTGCCTGG GGACTCAGGC CGCATGTGCA TCAACATGGA GTGGGGCGCC 
TTTGGGGACG ATGGCTCTCT GGCCATGCTC AGCACCCGCT TTGATGCAAG TGTGGACCAG 
GCGTCCATCA ACCCCGGCAA GCAGAGGTTT GAAAAGATGA TCAGCGGCAT GTACCTGGGG 
GAGATCGTCC GCCACATCCT TTTACATTTA ACCAGCCTTG GCGTTCTCTT CCGGGGCCAG 
CAGATCCAGC GCCTTCAGAC CAGGGACATC TTCAAGACCA AGTTCCTCTC TGAGATCGAA 
AGTGACAGCC TGGCCCTGCG GCAGGTCCGA GCCATCCTAG AGGATCTGGG GCTACCCCTG 
ACCTCAGATG ACGCCCTGAT GGTGCTAGAG GTGTGCCAGG CTGTGTCCCA GAGGGCTGCC 
CAGCTCTGTG GGGCGGGTGT AGCTGCCGTG GTGGAGAAGA TCCGGGAGAA CCGGGGCCTG 
GAAGAGCTGG CAGTGTCTGT GGGGGTGGAT GGAACGCTCT ACAAGCTGCA CCCGCGCTTC 
TCCAGCCTGG TGGCGGCCAC AGTGCGGGAG CTGGCCCCTC GCTGTGTGGT CACGTTCCTG 
CAGTCAGAGG ATGGGTCCGG CAAAGGTGCG GCCCTGGTCA CCGCTGTTGC CTGCCGCCTT 
GCGCAGTTGA CTCGTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864900.1
CDS	480..3080
Translation	MEQALRGQASPAPAVRMLPTYVGSTPHGTEQGDFVVLELGATGASLRVLWVTLTGIEGHRVEPRSQEFVIPQEVMLGAGQQLFDFAAHCLSEFLDAQPVNKQGLQLGFSFSFPCHQTGLDRSTLISWTKGFRCSGVEGQDVVQLLRDAIRRQGAYNIDVVAVVNDTVGTMMGCEPGVRPCEVGLVVDTGTNACYMEEARHVAVLDEDRGRVCVSVEWGSFSDDGALGPVLTTFDHTLDHESLNPGAQRFEKMIGGLYLGELVRLVLAHLARCGVLFGGCTSPALLSQGSILLEHVAEMEDPSTGAARVHAILQDLGLSPGASDVELVQHVCAAVCTRAAQLCAAALAAVLSCLQHSREQQTLQVAVATGGRVCERHPRFCSVLQGTVMLLAPECDVSLIPSVDGGGRGVAMVTAVAARLAAHRRLLEETLAPFRLNHDQLAAVQAQMRKAMAKGLRGEASSLRMLPTFVRATPDGSERGDFLALDLGGTNFRVLLVRVTTGVQITSEIYSIPETVAQGSGQQLFDHIVDCIVDFQQKQGLSGQSLPLGFTFSFPCRQLGLDQGILLNWTKGFKASDCEGQDVVSLLREAITRRQAVELNVVAIVNDTVGTMMSCGYEDPRCEIGLIVGTGTNACYMEELRNVAGVPGDSGRMCINMEWGAFGDDGSLAMLSTRFDASVDQASINPGKQRFEKMISGMYLGEIVRHILLHLTSLGVLFRGQQIQRLQTRDIFKTKFLSEIESDSLALRQVRAILEDLGLPLTSDDALMVLEVCQAVSQRAAQLCGAGVAAVVEKIRENRGLEELAVSVGVDGTLYKLHPRFSSLVAATVRELAPRCVVTFLQSEDGSGKGAALVTAVACRLAQLTRV

Target ORF information:

RefSeq Version	XM_017009411.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hexokinase 3 (HK3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009411.1

ORF Insert Sequence:

ATGGAGCAGG CGCTGAGGGG ACAGGCCAGC CCTGCCCCTG CGGTCCGGAT GCTGCCTACA 
TACGTGGGGT CCACCCCACA TGGCACTGAG CAAGGAGACT TCGTGGTGCT GGAGCTGGGG 
GCCACAGGGG CCTCACTGCG TGTTTTGTGG GTGACTCTAA CTGGCATTGA GGGGCATAGG 
GTGGAGCCCA GAAGCCAGGA GTTTGTGATC CCCCAAGAGG TGATGCTGGG TGCTGGCCAG 
CAGCTCTTTG ACTTTGCTGC CCACTGCCTG TCTGAGTTCC TGGATGCGCA GCCTGTGAAC 
AAACAGGGTC TGCAGCTTGG CTTCAGCTTC TCTTTCCCTT GTCACCAGAC GGGCTTGGAC 
AGGAGCACCC TCATTTCCTG GACCAAAGGT TTTAGGTGCA GTGGTGTGGA AGGCCAGGAT 
GTGGTCCAGC TGCTGAGAGA TGCCATTCGG AGGCAGGGGG CCTACAACAT CGACGTGGTT 
GCTGTGGTGA ACGACACAGT GGGCACCATG ATGGGCTGTG AGCCGGGGGT CAGGCCGTGT 
GAGGTTGGGC TAGTTGTAGA CACGGGCACC AACGCGTGTT ACATGGAGGA GGCACGGCAT 
GTGGCAGTGC TGGACGAAGA CCGGGGCCGC GTCTGCGTCA GCGTCGAGTG GGGCTCCTTC 
AGCGATGATG GGGCGCTGGG ACCAGTGCTG ACCACCTTCG ACCATACCCT GGACCATGAG 
TCCCTGAATC CTGGTGCTCA GAGGTTTGAG AAGATGATCG GAGGCCTGTA CCTGGGTGAG 
CTGGTGCGGC TGGTGCTGGC TCACTTGGCC CGGTGTGGGG TCCTCTTTGG TGGCTGCACC 
TCCCCTGCCC TGCTGAGCCA AGGCAGCATC CTCCTGGAAC ACGTGGCTGA GATGGAGGAC 
CCCTCTACTG GGGCAGCCCG TGTCCATGCT ATCCTGCAGG ACTTGGGCCT GAGCCCTGGG 
GCTTCGGATG TTGAGCTTGT GCAGCACGTC TGTGCGGCCG TGTGCACGCG GGCTGCCCAG 
CTCTGTGCTG CCGCCCTGGC CGCTGTTCTC TCCTGCCTCC AGCACAGCCG GGAGCAACAA 
ACACTCCAGG TTGCTGTGGC CACCGGAGGC CGAGTGTGTG AGCGGCACCC CAGGTTCTGC 
AGCGTCCTGC AGGGGACAGT GATGCTCCTG GCCCCGGAAT GCGATGTCTC CTTAATCCCC 
TCTGTGGATG GTGGTGGCCG GGGAGTGGCG ATGGTGACTG CCGTGGCTGC CCGTCTGGCT 
GCCCACCGGC GCCTGCTGGA GGAGACCCTG GCCCCATTCC GGTTGAACCA TGATCAACTG 
GCTGCGGTTC AGGCACAGAT GCGGAAGGCC ATGGCCAAGG GGCTCCGAGG GGAGGCCTCC 
TCCCTTCGCA TGCTGCCCAC TTTCGTCCGG GCCACCCCTG ACGGCAGCGA GCGAGGGGAT 
TTCCTGGCCC TGGACCTCGG GGGCACGAAC TTCCGTGTCC TCCTGGTACG TGTGACCACA 
GGCGTGCAGA TCACCAGCGA GATCTACTCC ATTCCCGAGA CTGTGGCCCA GGGTTCTGGG 
CAGCAGCTCT TTGACCACAT CGTGGACTGC ATCGTGGACT TCCAGCAGAA GCAGGGCCTG 
AGCGGGCAGA GCCTCCCACT GGGTTTTACC TTCTCCTTCC CATGTAGGCA GCTTGGCCTA 
GACCAGGGCA TCCTCCTGAA CTGGACCAAG GGTTTCAAGG CATCAGACTG CGAGGGCCAA 
GATGTCGTGA GTCTGTTGCG GGAAGCCATC ACTCGCAGAC AGGCAGTGGA GCTGAATGTG 
GTTGCCATTG TCAATGACAC GGTGGGGACC ATGATGTCCT GTGGCTATGA GGACCCCCGT 
TGCGAGATAG GCCTCATTGT CGGAACCGGC ACCAATGCCT GCTACATGGA GGAGCTCCGG 
AATGTGGCGG GCGTGCCTGG GGACTCAGGC CGCATGTGCA TCAACATGGA GTGGGGCGCC 
TTTGGGGACG ATGGCTCTCT GGCCATGCTC AGCACCCGCT TTGATGCAAG TGTGGACCAG 
GCGTCCATCA ACCCCGGCAA GCAGAGGTTT GAAAAGATGA TCAGCGGCAT GTACCTGGGG 
GAGATCGTCC GCCACATCCT TTTACATTTA ACCAGCCTTG GCGTTCTCTT CCGGGGCCAG 
CAGATCCAGC GCCTTCAGAC CAGGGACATC TTCAAGACCA AGTTCCTCTC TGAGATCGAA 
AGTGACAGCC TGGCCCTGCG GCAGGTCCGA GCCATCCTAG AGGATCTGGG GCTACCCCTG 
ACCTCAGATG ACGCCCTGAT GGTGCTAGAG GTGTGCCAGG CTGTGTCCCA GAGGGCTGCC 
CAGCTCTGTG GGGCGGGTGT AGCTGCCGTG GTGGAGAAGA TCCGGGAGAA CCGGGGCCTG 
GAAGAGCTGG CAGTGTCTGT GGGGGTGGAT GGAACGCTCT ACAAGCTGCA CCCGCGCTTC 
TCCAGCCTGG TGGCGGCCAC AGTGCGGGAG CTGGCCCCTC GCTGTGTGGT CACGTTCCTG 
CAGTCAGAGG ATGGGTCCGG CAAAGGTGCG GCCCTGGTCA CCGCTGTTGC CTGCCGCCTT 
GCGCAGTTGA CTCGTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human gingiva transcriptome during wound healing.
Journal of clinical periodontology44(4)394-402(2017 Apr)
Wang Y,Tatakis DN

High 18F-FDG uptake in PMAH correlated with normal expression of Glut1, HK1, HK2, and HK3.
Acta radiologica (Stockholm, Sweden : 1987)57(3)370-7(2016 Mar)
Cavalcante IP,Zerbini MC,Alencar GA,Mariani Bde P,Buchpiguel CA,Almeida MQ,Mendonca BB,Fragoso MC

[Evaluation of Gene Expression of Hexokinases in Colorectal Cancer with the Use of Bioinformatics Methods].
Biofizika60(6)1050-6(2015 Nov-Dec)
Krasnov GS,Dmitriev AA,Sadtritdinova AF,Fedorova MS,Snezhkina AV,Melnikova NV,Poteryakhina AV,Nyushko KM,Belyakov MM,Kaprin AD,Zaretsky AR,Kudryavtseva AV

Identification of key genes affecting disease free survival time of pediatric acute lymphoblastic leukemia based on bioinformatic analysis.
Blood cells, molecules & diseases54(1)38-43(2015 Jan)
Gao HY,Luo XG,Chen X,Wang JH

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HK3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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