Themis cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Themis
Entrez Gene ID	210757
Full Name	thymocyte selection associated
Synonyms	E430004N04Rik,Gasp,Spot,Tsepa,thylex
General protein information	Preferred Names thymocyte selection associated Names protein THEMIS grb2-associating protein signaling phosphoprotein specific for T cells thymocyte selection pathway associated thymocyte-expressed molecule involved in selection thymus lymphocyte exclusively expressed
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 A4
Summary	This gene encodes a protein that plays a regulatory role in both positive and negative T-cell selection during late thymocyte development. The protein functions through T-cell antigen receptor signaling, and is necessary for proper lineage commitment and maturation of T-cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_178666.6	NP_848781.1	protein THEMIS isoform 1
NM_001305663.1	NP_001292592.1	protein THEMIS isoform 2
NM_001374699.1	NP_001361628.1	protein THEMIS isoform 4
XM_006512643.1	XP_006512706.1	protein THEMIS isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	THEMIS	NP_001158157.1
Danio rerio (zebrafish)	themis	XP_683025.3
Pan troglodytes (chimpanzee)	THEMIS	XP_003311524.1
Canis lupus familiaris (dog)	THEMIS	XP_541237.4
Bos taurus (cattle)	THEMIS	NP_001091585.1
Gallus gallus (chicken)	THEMIS	XP_001232459.3
Xenopus tropicalis (tropical clawed frog)	themis	XP_002940439.2
Rattus norvegicus (Norway rat)	Themis	XP_006227737.1
Mus musculus (house mouse)	Themis	NP_848781.1

Gene Ontology
Bibliography

Related articles in PubMed

Grb2-Mediated Recruitment of USP9X to LAT Enhances Themis Stability following Thymic Selection.
Garreau A, Blaize G, Argenty J, Rouqui? N, Tourd?s A, Wood SA, Saoudi A, Lesourne R
Journal of immunology (Baltimore, Md. : 1950)199(8)2758-2766(2017 Oct)

Proteomic Analysis of Regulatory T Cells Reveals the Importance of Themis1 in the Control of Their Suppressive Function.
Duguet F, Locard-Paulet M, Marcellin M, Chaoui K, Bernard I, Andreoletti O, Lesourne R, Burlet-Schiltz O, Gonzalez de Peredo A, Saoudi A
Molecular & cellular proteomics : MCP16(8)1416-1432(2017 Aug)

THEMIS enhances TCR signaling and enables positive selection by selective inhibition of the phosphatase SHP-1.
Choi S, Warzecha C, Zvezdova E, Lee J, Argenty J, Lesourne R, Aravind L, Love PE
Nature immunology18(4)433-441(2017 Apr)

Themis1 enhances T cell receptor signaling during thymocyte development by promoting Vav1 activity and Grb2 stability.
Zvezdova E, Mikolajczak J, Garreau A, Marcellin M, Rigal L, Lee J, Choi S, Blaize G, Argenty J, Familiades J, Li L, Gonzalez de Peredo A, Burlet-Schiltz O, Love PE, Lesourne R
Science signaling9(428)ra51(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the positive selection of immature CD4+CD8+ double-positive thymocytes and their commitment to the CD4+CD8- single-positive stage are impaired in Themis-/- mice
Title: Grb2-Mediated Recruitment of USP9X to LAT Enhances Themis Stability following Thymic Selection.

this study demonstrates the potential role of Themis1 in the suppressive functions of regulatory T cells.
Title: Proteomic Analysis of Regulatory T Cells Reveals the Importance of Themis1 in the Control of Their Suppressive Function.

we found that THEMIS directly regulated the catalytic activity of the tyrosine phosphatase SHP-1.
Title: THEMIS enhances TCR signaling and enables positive selection by selective inhibition of the phosphatase SHP-1.

Themis1 acts as a positive regulator of TCR signaling during thymocyte development by promoting Vav1 activity and Grb2 stability
Title: Themis1 enhances T cell receptor signaling during thymocyte development by promoting Vav1 activity and Grb2 stability.

the THEMIS:SHP complex dampens early TCR signalling by a previously unknown molecular mechanism that favours T-cell survival.
Title: A THEMIS:SHP1 complex promotes T-cell survival.

The following Themis gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Themis cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18241	NM_178666.6 Latest version!	Mus musculus thymocyte selection associated (Themis), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu72739	NM_001305663.1 Latest version!	Mus musculus thymocyte selection associated (Themis), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu43806	XM_006512643.1 Latest version!	Mus musculus thymocyte selection associated (Themis), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu90618	NM_001374699.1 Latest version!	Mus musculus thymocyte selection associated (Themis), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Themis ( NM_178666.6 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_848781.1 Mus musculus thymocyte selection associated (Themis), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18241
Clone ID Related Accession (Same CDS sequence)	NM_178666.6
Accession Version	NM_178666.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-23
Organism	Mus musculus(house mouse)
Product	protein THEMIS isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB638055.1, AK040594.1, AK169558.1 and AA967480.1. On Mar 26, 2015 this sequence version replaced NM_178666.5. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK088136.1, AK041562.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849388 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTTAT CTCTGGAAGA ATTTGTCTAC TCCCTTGACC TGAGGACCCT CCCCAGGGTC 
CTAGAAATCC AGTCAGGCAT CTACTTTGAA GGGTCTGTTT ATGAGATGTT TGGCAATGAA 
TGCTGTCTGT CAACAGGAGA AGTGATTAAA ATCACCGGTC TCAAAATTAA GAAGATGATG 
GCTGAAATTT GTGAGGGCGC TATTGGAGGT TGTGAGTCTC AGAAGCCTTT TGAGCTGCCC 
ATGAATTTTC CAGGTCTCTT TAAGGTCATG GCTGATAAAA CTCCATACCT GTCAATTGAA 
GAAATCACAA GAACCGTTAA TATTGGACCC AGCAGACTAG GTCATCCTTG CTTTTATCAC 
CTGAAGGATA TAAAACTAGA GAATCTCATT ATTAAGCAGG GTGAGCCAAT CCGGTTCAAC 
TCAGTTGAAG AGATTAATGG AGAAACGCTG GTGAATTGTG GTGTGGTAAG GAATCATCAG 
AGCCACTCAT TTACTTTGCC ATTGTCACAA GAAGGAGAAT TTTATGAGTG TGAGGATGAA 
CATATCTATA CCCTAAAAGA AATTGTTGAA TGGAAGATTC CCAAGAACAG AACACGGACA 
GTGAAACTTA CAGATTTCTC GAATAAGTGG GACTCAACCA ATCCATTCCC AGAAGACTTT 
TATGGTACAC TGATTCTCAA ACCTGTTTAT GAAATCCAAG GTGTGCTGAA ATTTCAAAAG 
GACATAGTCC GGATTCTCCC AAGTTTAGAC GTTGAAGTCA AAGATATTAC TGATTCTTAT 
GATGCTAACT GGTTTCTTCA GTTGCTGTCT ACGGACGACC TTTTTGAAAT GACCAGCAAA 
GAGTTTCCTG TAGTAGCTGA AGTTGTCGAA ATATCTCAAG GGAACCACCT GCCCCAAAGT 
ATTTTGCAGC GAGAGAAAAC CATTGTCATC CACAAAAAGT ACCAGGCTTC GAGGATCTTA 
GCTTCAGAAA TTCGCAGCAA TTTCCCTAAA AGACACTTCT TGATCCCTAT TAGCTACAAA 
GGCAAGTTCA AAAGAAGACC ACGGGAGTTC CCCACGGCCT ATGACCTGCA GATAGCTAAG 
AGCAGGAAAG AAACTCTCCA CGTGGTGGCC ACCAAAGCTT TCCATACACT TCACAAGGAG 
CTGTCCCCTG TGTCTGTTGG AGACCAGTTT CTAGTGCATC ATTCAGAGAC CACAGAAGTT 
GTCTTTGAGG GGACAAGAAA AGTAAACGTT CTGACCTGTG AAAAAGTCCT CAATAAGACC 
CGTGAAGATG CACAGCTTCC TCTATACATG GAAGGAGGTT TTGTAGAGGT GATTCATGAT 
AAGAAACAGT ATCAGATTTC CGAGCTCTGT ACCCAGTTTT GCTGGCCCTT CAATGTGAAG 
GTGGCTGTGA GAGATCTCTC CATTAAAGAT GACATTTTGG CAGCTACCCC AGGACTTCAG 
TTAGAGGAGG ACATCACAGA TTCTTATCTA CTTATCAGTG ACTTTGCAAA TCCTGAGGAA 
TGTTGGGAAA TTCCCATGAG CCGCTTGAAT ATGACTGTTC GACTAGTTAA TGGTAGCAGT 
TTGCCTGCAG ATGCAGGGCT ACTTCAAGTC AGGAGTTTTG TAGAAGAGAT TACAGAAGAA 
CAGTATTACA TGATGAGGAG GTACGAAAGT TCTCTATCAC ATCCCCCACC TCGCCCTCCC 
AAACATCCCT CAGCTGAGGA AATGAAGCTC ACCTTGCTCA GCTTAGCAGA AGAGAGAACA 
ATAAATCTGC CCAAGTCTCT CAAGAGTCAC CATGTAGACA GACCCAAGAA GCTTCCCTCA 
GATGAAAGTG GCCAGGATTC AAGAGCTCCA GTTGGTTTTC AGAATGATGT GGCTGATGTG 
GAGAGACAGA AGAGCAAGCA TGGGCCACTG CAGCCGCAAG CACCACTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_848781.1
CDS	301..2211
Translation	MALSLEEFVYSLDLRTLPRVLEIQSGIYFEGSVYEMFGNECCLSTGEVIKITGLKIKKMMAEICEGAIGGCESQKPFELPMNFPGLFKVMADKTPYLSIEEITRTVNIGPSRLGHPCFYHLKDIKLENLIIKQGEPIRFNSVEEINGETLVNCGVVRNHQSHSFTLPLSQEGEFYECEDEHIYTLKEIVEWKIPKNRTRTVKLTDFSNKWDSTNPFPEDFYGTLILKPVYEIQGVLKFQKDIVRILPSLDVEVKDITDSYDANWFLQLLSTDDLFEMTSKEFPVVAEVVEISQGNHLPQSILQREKTIVIHKKYQASRILASEIRSNFPKRHFLIPISYKGKFKRRPREFPTAYDLQIAKSRKETLHVVATKAFHTLHKELSPVSVGDQFLVHHSETTEVVFEGTRKVNVLTCEKVLNKTREDAQLPLYMEGGFVEVIHDKKQYQISELCTQFCWPFNVKVAVRDLSIKDDILAATPGLQLEEDITDSYLLISDFANPEECWEIPMSRLNMTVRLVNGSSLPADAGLLQVRSFVEEITEEQYYMMRRYESSLSHPPPRPPKHPSAEEMKLTLLSLAEERTINLPKSLKSHHVDRPKKLPSDESGQDSRAPVGFQNDVADVERQKSKHGPLQPQAPL

Target ORF information:

RefSeq Version	NM_178666.6
Organism	Mus musculus(house mouse)
Definition	Mus musculus thymocyte selection associated (Themis), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178666.6

ORF Insert Sequence:

ATGGCTTTAT CTCTGGAAGA ATTTGTCTAC TCCCTTGACC TGAGGACCCT CCCCAGGGTC 
CTAGAAATCC AGTCAGGCAT CTACTTTGAA GGGTCTGTTT ATGAGATGTT TGGCAATGAA 
TGCTGTCTGT CAACAGGAGA AGTGATTAAA ATCACCGGTC TCAAAATTAA GAAGATGATG 
GCTGAAATTT GTGAGGGCGC TATTGGAGGT TGTGAGTCTC AGAAGCCTTT TGAGCTGCCC 
ATGAATTTTC CAGGTCTCTT TAAGGTCATG GCTGATAAAA CTCCATACCT GTCAATTGAA 
GAAATCACAA GAACCGTTAA TATTGGACCC AGCAGACTAG GTCATCCTTG CTTTTATCAC 
CTGAAGGATA TAAAACTAGA GAATCTCATT ATTAAGCAGG GTGAGCCAAT CCGGTTCAAC 
TCAGTTGAAG AGATTAATGG AGAAACGCTG GTGAATTGTG GTGTGGTAAG GAATCATCAG 
AGCCACTCAT TTACTTTGCC ATTGTCACAA GAAGGAGAAT TTTATGAGTG TGAGGATGAA 
CATATCTATA CCCTAAAAGA AATTGTTGAA TGGAAGATTC CCAAGAACAG AACACGGACA 
GTGAAACTTA CAGATTTCTC GAATAAGTGG GACTCAACCA ATCCATTCCC AGAAGACTTT 
TATGGTACAC TGATTCTCAA ACCTGTTTAT GAAATCCAAG GTGTGCTGAA ATTTCAAAAG 
GACATAGTCC GGATTCTCCC AAGTTTAGAC GTTGAAGTCA AAGATATTAC TGATTCTTAT 
GATGCTAACT GGTTTCTTCA GTTGCTGTCT ACGGACGACC TTTTTGAAAT GACCAGCAAA 
GAGTTTCCTG TAGTAGCTGA AGTTGTCGAA ATATCTCAAG GGAACCACCT GCCCCAAAGT 
ATTTTGCAGC GAGAGAAAAC CATTGTCATC CACAAAAAGT ACCAGGCTTC GAGGATCTTA 
GCTTCAGAAA TTCGCAGCAA TTTCCCTAAA AGACACTTCT TGATCCCTAT TAGCTACAAA 
GGCAAGTTCA AAAGAAGACC ACGGGAGTTC CCCACGGCCT ATGACCTGCA GATAGCTAAG 
AGCAGGAAAG AAACTCTCCA CGTGGTGGCC ACCAAAGCTT TCCATACACT TCACAAGGAG 
CTGTCCCCTG TGTCTGTTGG AGACCAGTTT CTAGTGCATC ATTCAGAGAC CACAGAAGTT 
GTCTTTGAGG GGACAAGAAA AGTAAACGTT CTGACCTGTG AAAAAGTCCT CAATAAGACC 
CGTGAAGATG CACAGCTTCC TCTATACATG GAAGGAGGTT TTGTAGAGGT GATTCATGAT 
AAGAAACAGT ATCAGATTTC CGAGCTCTGT ACCCAGTTTT GCTGGCCCTT CAATGTGAAG 
GTGGCTGTGA GAGATCTCTC CATTAAAGAT GACATTTTGG CAGCTACCCC AGGACTTCAG 
TTAGAGGAGG ACATCACAGA TTCTTATCTA CTTATCAGTG ACTTTGCAAA TCCTGAGGAA 
TGTTGGGAAA TTCCCATGAG CCGCTTGAAT ATGACTGTTC GACTAGTTAA TGGTAGCAGT 
TTGCCTGCAG ATGCAGGGCT ACTTCAAGTC AGGAGTTTTG TAGAAGAGAT TACAGAAGAA 
CAGTATTACA TGATGAGGAG GTACGAAAGT TCTCTATCAC ATCCCCCACC TCGCCCTCCC 
AAACATCCCT CAGCTGAGGA AATGAAGCTC ACCTTGCTCA GCTTAGCAGA AGAGAGAACA 
ATAAATCTGC CCAAGTCTCT CAAGAGTCAC CATGTAGACA GACCCAAGAA GCTTCCCTCA 
GATGAAAGTG GCCAGGATTC AAGAGCTCCA GTTGGTTTTC AGAATGATGT GGCTGATGTG 
GAGAGACAGA AGAGCAAGCA TGGGCCACTG CAGCCGCAAG CACCACTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Themis ( NM_001305663.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001292592.1 Mus musculus thymocyte selection associated (Themis), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu72739
Clone ID Related Accession (Same CDS sequence)	NM_001305663.1
Accession Version	NM_001305663.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-23
Organism	Mus musculus(house mouse)
Product	protein THEMIS isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB638055.1, AK040594.1, AK169558.1, AC159472.6 and AC153798.1. Transcript Variant: This variant (2) uses an alternate 5' exon and lacks a 3' coding exon compared to variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). The encoded isoform (2) has a shorter N-terminus and a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK169558.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849383, SAMN00849385 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGATA AAACTCCATA CCTGTCAATT GAAGAAATCA CAAGAACCGT TAATATTGGA 
CCCAGCAGAC TAGGTCATCC TTGCTTTTAT CACCTGAAGG ATATAAAACT AGAGAATCTC 
ATTATTAAGC AGGGTGAGCC AATCCGGTTC AACTCAGTTG AAGAGATTAA TGGAGAAACG 
CTGGTGAATT GTGGTGTGGT AAGGAATCAT CAGAGCCACT CATTTACTTT GCCATTGTCA 
CAAGAAGGAG AATTTTATGA GTGTGAGGAT GAACATATCT ATACCCTAAA AGAAATTGTT 
GAATGGAAGA TTCCCAAGAA CAGAACACGG ACAGTGAAAC TTACAGATTT CTCGAATAAG 
TGGGACTCAA CCAATCCATT CCCAGAAGAC TTTTATGGTA CACTGATTCT CAAACCTGTT 
TATGAAATCC AAGGTGTGCT GAAATTTCAA AAGGACATAG TCCGGATTCT CCCAAGTTTA 
GACGTTGAAG TCAAAGATAT TACTGATTCT TATGATGCTA ACTGGTTTCT TCAGTTGCTG 
TCTACGGACG ACCTTTTTGA AATGACCAGC AAAGAGTTTC CTGTAGTAGC TGAAGTTGTC 
GAAATATCTC AAGGGAACCA CCTGCCCCAA AGTATTTTGC AGCGAGAGAA AACCATTGTC 
ATCCACAAAA AGTACCAGGC TTCGAGGATC TTAGCTTCAG AAATTCGCAG CAATTTCCCT 
AAAAGACACT TCTTGATCCC TATTAGCTAC AAAGGCAAGT TCAAAAGAAG ACCACGGGAG 
TTCCCCACGG CCTATGACCT GCAGATAGCT AAGAGCAGGA AAGAAACTCT CCACGTGGTG 
GCCACCAAAG CTTTCCATAC ACTTCACAAG GAGCTGTCCC CTGTGTCTGT TGGAGACCAG 
TTTCTAGTGC ATCATTCAGA GACCACAGAA GTTGTCTTTG AGGGGACAAG AAAAGTAAAC 
GTTCTGACCT GTGAAAAAGT CCTCAATAAG ACCCGTGAAG ATGCACAGCT TCCTCTATAC 
ATGGAAGGAG GTTTTGTAGA GGTGATTCAT GATAAGAAAC AGTATCAGAT TTCCGAGCTC 
TGTACCCAGT TTTGCTGGCC CTTCAATGTG AAGGTGGCTG TGAGAGATCT CTCCATTAAA 
GATGACATTT TGGCAGCTAC CCCAGGACTT CAGTTAGAGG AGGACATCAC AGATTCTTAT 
CTACTTATCA GTGACTTTGC AAATCCTGAG GAATGTTGGG AAATTCCCAT GAGCCGCTTG 
AATATGACTG TTCGACTAGT TAATGGTAGC AGTTTGCCTG CAGATGCAGG GCTACTTCAA 
GTCAGGAGTT TTGTAGAAGA GATTACAGAA GAACAGTATT ACATGATGAG GAGGTACGAA 
AGTTCTCTAT CACATCCCCC ACCTCGCCCT CCCAAACATC CCTCAGCTGA GGAAATGAAG 
CTCACCTTGC TCAGCTTAGC AGAAGAGAGA ACAATAAATC TGCCCAAGTC TCTCAAGAAC 
TCTTCAAAAC TGAAAAACAT CAAAAATAAC AAGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292592.1
CDS	730..2268
Translation	MADKTPYLSIEEITRTVNIGPSRLGHPCFYHLKDIKLENLIIKQGEPIRFNSVEEINGETLVNCGVVRNHQSHSFTLPLSQEGEFYECEDEHIYTLKEIVEWKIPKNRTRTVKLTDFSNKWDSTNPFPEDFYGTLILKPVYEIQGVLKFQKDIVRILPSLDVEVKDITDSYDANWFLQLLSTDDLFEMTSKEFPVVAEVVEISQGNHLPQSILQREKTIVIHKKYQASRILASEIRSNFPKRHFLIPISYKGKFKRRPREFPTAYDLQIAKSRKETLHVVATKAFHTLHKELSPVSVGDQFLVHHSETTEVVFEGTRKVNVLTCEKVLNKTREDAQLPLYMEGGFVEVIHDKKQYQISELCTQFCWPFNVKVAVRDLSIKDDILAATPGLQLEEDITDSYLLISDFANPEECWEIPMSRLNMTVRLVNGSSLPADAGLLQVRSFVEEITEEQYYMMRRYESSLSHPPPRPPKHPSAEEMKLTLLSLAEERTINLPKSLKNSSKLKNIKNNKT

Target ORF information:

RefSeq Version	NM_001305663.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thymocyte selection associated (Themis), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305663.1

ORF Insert Sequence:

ATGGCTGATA AAACTCCATA CCTGTCAATT GAAGAAATCA CAAGAACCGT TAATATTGGA 
CCCAGCAGAC TAGGTCATCC TTGCTTTTAT CACCTGAAGG ATATAAAACT AGAGAATCTC 
ATTATTAAGC AGGGTGAGCC AATCCGGTTC AACTCAGTTG AAGAGATTAA TGGAGAAACG 
CTGGTGAATT GTGGTGTGGT AAGGAATCAT CAGAGCCACT CATTTACTTT GCCATTGTCA 
CAAGAAGGAG AATTTTATGA GTGTGAGGAT GAACATATCT ATACCCTAAA AGAAATTGTT 
GAATGGAAGA TTCCCAAGAA CAGAACACGG ACAGTGAAAC TTACAGATTT CTCGAATAAG 
TGGGACTCAA CCAATCCATT CCCAGAAGAC TTTTATGGTA CACTGATTCT CAAACCTGTT 
TATGAAATCC AAGGTGTGCT GAAATTTCAA AAGGACATAG TCCGGATTCT CCCAAGTTTA 
GACGTTGAAG TCAAAGATAT TACTGATTCT TATGATGCTA ACTGGTTTCT TCAGTTGCTG 
TCTACGGACG ACCTTTTTGA AATGACCAGC AAAGAGTTTC CTGTAGTAGC TGAAGTTGTC 
GAAATATCTC AAGGGAACCA CCTGCCCCAA AGTATTTTGC AGCGAGAGAA AACCATTGTC 
ATCCACAAAA AGTACCAGGC TTCGAGGATC TTAGCTTCAG AAATTCGCAG CAATTTCCCT 
AAAAGACACT TCTTGATCCC TATTAGCTAC AAAGGCAAGT TCAAAAGAAG ACCACGGGAG 
TTCCCCACGG CCTATGACCT GCAGATAGCT AAGAGCAGGA AAGAAACTCT CCACGTGGTG 
GCCACCAAAG CTTTCCATAC ACTTCACAAG GAGCTGTCCC CTGTGTCTGT TGGAGACCAG 
TTTCTAGTGC ATCATTCAGA GACCACAGAA GTTGTCTTTG AGGGGACAAG AAAAGTAAAC 
GTTCTGACCT GTGAAAAAGT CCTCAATAAG ACCCGTGAAG ATGCACAGCT TCCTCTATAC 
ATGGAAGGAG GTTTTGTAGA GGTGATTCAT GATAAGAAAC AGTATCAGAT TTCCGAGCTC 
TGTACCCAGT TTTGCTGGCC CTTCAATGTG AAGGTGGCTG TGAGAGATCT CTCCATTAAA 
GATGACATTT TGGCAGCTAC CCCAGGACTT CAGTTAGAGG AGGACATCAC AGATTCTTAT 
CTACTTATCA GTGACTTTGC AAATCCTGAG GAATGTTGGG AAATTCCCAT GAGCCGCTTG 
AATATGACTG TTCGACTAGT TAATGGTAGC AGTTTGCCTG CAGATGCAGG GCTACTTCAA 
GTCAGGAGTT TTGTAGAAGA GATTACAGAA GAACAGTATT ACATGATGAG GAGGTACGAA 
AGTTCTCTAT CACATCCCCC ACCTCGCCCT CCCAAACATC CCTCAGCTGA GGAAATGAAG 
CTCACCTTGC TCAGCTTAGC AGAAGAGAGA ACAATAAATC TGCCCAAGTC TCTCAAGAAC 
TCTTCAAAAC TGAAAAACAT CAAAAATAAC AAGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Themis ( XM_006512643.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512706.1 Mus musculus thymocyte selection associated (Themis), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu43806
Clone ID Related Accession (Same CDS sequence)	XM_006512643.1
Accession Version	XM_006512643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein THEMIS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATA AAACTCCATA CCTGTCAATT GAAGAAATCA CAAGAACCGT TAATATTGGA 
CCCAGCAGAC TAGGTCATCC TTGCTTTTAT CACCTGAAGG ATATAAAACT AGAGAATCTC 
ATTATTAAGC AGGGTGAGCC AATCCGGTTC AACTCAGTTG AAGAGATTAA TGGAGAAACG 
CTGGTGAATT GTGGTGTGGT AAGGAATCAT CAGAGCCACT CATTTACTTT GCCATTGTCA 
CAAGAAGGAG AATTTTATGA GTGTGAGGAT GAACATATCT ATACCCTAAA AGAAATTGTT 
GAATGGAAGA TTCCCAAGAA CAGAACACGG ACAGTGAAAC TTACAGATTT CTCGAATAAG 
TGGGACTCAA CCAATCCATT CCCAGAAGAC TTTTATGGTA CACTGATTCT CAAACCTGTT 
TATGAAATCC AAGGTGTGCT GAAATTTCAA AAGGACATAG TCCGGATTCT CCCAAGTTTA 
GACGTTGAAG TCAAAGATAT TACTGATTCT TATGATGCTA ACTGGTTTCT TCAGTTGCTG 
TCTACGGACG ACCTTTTTGA AATGACCAGC AAAGAGTTTC CTGTAGTAGC TGAAGTTGTC 
GAAATATCTC AAGGGAACCA CCTGCCCCAA AGTATTTTGC AGCGAGAGAA AACCATTGTC 
ATCCACAAAA AGTACCAGGC TTCGAGGATC TTAGCTTCAG AAATTCGCAG CAATTTCCCT 
AAAAGACACT TCTTGATCCC TATTAGCTAC AAAGGCAAGT TCAAAAGAAG ACCACGGGAG 
TTCCCCACGG CCTATGACCT GCAGATAGCT AAGAGCAGGA AAGAAACTCT CCACGTGGTG 
GCCACCAAAG CTTTCCATAC ACTTCACAAG GAGCTGTCCC CTGTGTCTGT TGGAGACCAG 
TTTCTAGTGC ATCATTCAGA GACCACAGAA GTTGTCTTTG AGGGGACAAG AAAAGTAAAC 
GTTCTGACCT GTGAAAAAGT CCTCAATAAG ACCCGTGAAG ATGCACAGCT TCCTCTATAC 
ATGGAAGGAG GTTTTGTAGA GGTGATTCAT GATAAGAAAC AGTATCAGAT TTCCGAGCTC 
TGTACCCAGT TTTGCTGGCC CTTCAATGTG AAGGTGGCTG TGAGAGATCT CTCCATTAAA 
GATGACATTT TGGCAGCTAC CCCAGGACTT CAGTTAGAGG AGGACATCAC AGATTCTTAT 
CTACTTATCA GTGACTTTGC AAATCCTGAG GAATGTTGGG AAATTCCCAT GAGCCGCTTG 
AATATGACTG TTCGACTAGT TAATGGTAGC AGTTTGCCTG CAGATGCAGG GCTACTTCAA 
GTCAGGAGTT TTGTAGAAGA GATTACAGAA GAACAGTATT ACATGATGAG GAGGTACGAA 
AGTTCTCTAT CACATCCCCC ACCTCGCCCT CCCAAACATC CCTCAGCTGA GGAAATGAAG 
CTCACCTTGC TCAGCTTAGC AGAAGAGAGA ACAATAAATC TGCCCAAGTC TCTCAAGAGT 
CACCATGTAG ACAGACCCAA GAAGCTTCCC TCAGATGAAA GTGGCCAGGA TTCAAGAGCT 
CCAGTTGGTT TTCAGAATGA TGTGGCTGAT GTGGAGAGAC AGAAGAGCAA GCATGGGCCA 
CTGCAGCCGC AAGCACCACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512706.1
CDS	119..1762
Translation	MADKTPYLSIEEITRTVNIGPSRLGHPCFYHLKDIKLENLIIKQGEPIRFNSVEEINGETLVNCGVVRNHQSHSFTLPLSQEGEFYECEDEHIYTLKEIVEWKIPKNRTRTVKLTDFSNKWDSTNPFPEDFYGTLILKPVYEIQGVLKFQKDIVRILPSLDVEVKDITDSYDANWFLQLLSTDDLFEMTSKEFPVVAEVVEISQGNHLPQSILQREKTIVIHKKYQASRILASEIRSNFPKRHFLIPISYKGKFKRRPREFPTAYDLQIAKSRKETLHVVATKAFHTLHKELSPVSVGDQFLVHHSETTEVVFEGTRKVNVLTCEKVLNKTREDAQLPLYMEGGFVEVIHDKKQYQISELCTQFCWPFNVKVAVRDLSIKDDILAATPGLQLEEDITDSYLLISDFANPEECWEIPMSRLNMTVRLVNGSSLPADAGLLQVRSFVEEITEEQYYMMRRYESSLSHPPPRPPKHPSAEEMKLTLLSLAEERTINLPKSLKSHHVDRPKKLPSDESGQDSRAPVGFQNDVADVERQKSKHGPLQPQAPL

Target ORF information:

RefSeq Version	XM_006512643.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thymocyte selection associated (Themis), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512643.1

ORF Insert Sequence:

ATGGCTGATA AAACTCCATA CCTGTCAATT GAAGAAATCA CAAGAACCGT TAATATTGGA 
CCCAGCAGAC TAGGTCATCC TTGCTTTTAT CACCTGAAGG ATATAAAACT AGAGAATCTC 
ATTATTAAGC AGGGTGAGCC AATCCGGTTC AACTCAGTTG AAGAGATTAA TGGAGAAACG 
CTGGTGAATT GTGGTGTGGT AAGGAATCAT CAGAGCCACT CATTTACTTT GCCATTGTCA 
CAAGAAGGAG AATTTTATGA GTGTGAGGAT GAACATATCT ATACCCTAAA AGAAATTGTT 
GAATGGAAGA TTCCCAAGAA CAGAACACGG ACAGTGAAAC TTACAGATTT CTCGAATAAG 
TGGGACTCAA CCAATCCATT CCCAGAAGAC TTTTATGGTA CACTGATTCT CAAACCTGTT 
TATGAAATCC AAGGTGTGCT GAAATTTCAA AAGGACATAG TCCGGATTCT CCCAAGTTTA 
GACGTTGAAG TCAAAGATAT TACTGATTCT TATGATGCTA ACTGGTTTCT TCAGTTGCTG 
TCTACGGACG ACCTTTTTGA AATGACCAGC AAAGAGTTTC CTGTAGTAGC TGAAGTTGTC 
GAAATATCTC AAGGGAACCA CCTGCCCCAA AGTATTTTGC AGCGAGAGAA AACCATTGTC 
ATCCACAAAA AGTACCAGGC TTCGAGGATC TTAGCTTCAG AAATTCGCAG CAATTTCCCT 
AAAAGACACT TCTTGATCCC TATTAGCTAC AAAGGCAAGT TCAAAAGAAG ACCACGGGAG 
TTCCCCACGG CCTATGACCT GCAGATAGCT AAGAGCAGGA AAGAAACTCT CCACGTGGTG 
GCCACCAAAG CTTTCCATAC ACTTCACAAG GAGCTGTCCC CTGTGTCTGT TGGAGACCAG 
TTTCTAGTGC ATCATTCAGA GACCACAGAA GTTGTCTTTG AGGGGACAAG AAAAGTAAAC 
GTTCTGACCT GTGAAAAAGT CCTCAATAAG ACCCGTGAAG ATGCACAGCT TCCTCTATAC 
ATGGAAGGAG GTTTTGTAGA GGTGATTCAT GATAAGAAAC AGTATCAGAT TTCCGAGCTC 
TGTACCCAGT TTTGCTGGCC CTTCAATGTG AAGGTGGCTG TGAGAGATCT CTCCATTAAA 
GATGACATTT TGGCAGCTAC CCCAGGACTT CAGTTAGAGG AGGACATCAC AGATTCTTAT 
CTACTTATCA GTGACTTTGC AAATCCTGAG GAATGTTGGG AAATTCCCAT GAGCCGCTTG 
AATATGACTG TTCGACTAGT TAATGGTAGC AGTTTGCCTG CAGATGCAGG GCTACTTCAA 
GTCAGGAGTT TTGTAGAAGA GATTACAGAA GAACAGTATT ACATGATGAG GAGGTACGAA 
AGTTCTCTAT CACATCCCCC ACCTCGCCCT CCCAAACATC CCTCAGCTGA GGAAATGAAG 
CTCACCTTGC TCAGCTTAGC AGAAGAGAGA ACAATAAATC TGCCCAAGTC TCTCAAGAGT 
CACCATGTAG ACAGACCCAA GAAGCTTCCC TCAGATGAAA GTGGCCAGGA TTCAAGAGCT 
CCAGTTGGTT TTCAGAATGA TGTGGCTGAT GTGGAGAGAC AGAAGAGCAA GCATGGGCCA 
CTGCAGCCGC AAGCACCACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Themis ( NM_001374699.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001361628.1 Mus musculus thymocyte selection associated (Themis), transcript variant 4, mRNA.

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CloneID	OMu90618
Clone ID Related Accession (Same CDS sequence)	NM_001374699.1
Accession Version	NM_001374699.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	protein THEMIS isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC152983.2 and AC159472.6. On Oct 9, 2019 this sequence version replaced XM_030244987.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK138701.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTTAT CTCTGGAAGA ATTTGTCTAC TCCCTTGACC TGAGGACCCT CCCCAGGGTC 
CTAGAAATCC AGTCAGGCAT CTACTTTGAA GGGTCTGTTT ATGAGATGTT TGGCAATGAA 
TGCTGTCTGT CAACAGGAGA AGTGATTAAA ATCACCGGTC TCAAAATTAA GAAGATGATG 
GCTGAAATTT GTGAGGGCGC TATTGGAGGT TGTGAGTCTC AGAAGCCTTT TGAGCTGCCC 
ATGAATTTTC CAGGTCTCTT TAAGGTCATG GCTGATAAAA CTCCATACCT GTCAATTGAA 
GAAATCACAA GAACCGTTAA TATTGGACCC AGCAGACTAG GTCATCCTTG CTTTTATCAC 
CTGAAGGATA TAAAACTAGA GAATCTCATT ATTAAGCAGG GTGAGCCAAT CCGGTTCAAC 
TCAGTTGAAG AGATTAATGG AGAAACGCTG GTGAATTGTG GTGTGGTAAG GAATCATCAG 
AGCCACTCAT TTACTTTGCC ATTGTCACAA GAAGGAGAAT TTTATGAGTG TGAGGATGAA 
CATATCTATA CCCTAAAAGA AATTGTTGAA TGGAAGATTC CCAAGAACAG AACACGGACA 
GTGAAACTTA CAGATTTCTC GAATAAGTGG GACTCAACCA ATCCATTCCC AGAAGACTTT 
TATGGTACAC TGATTCTCAA ACCTGTTTAT GAAATCCAAG GTGTGCTGAA ATTTCAAAAG 
GACATAGTCC GGATTCTCCC AAGTTTAGAC GTTGAAGTCA AAGATATTAC TGATTCTTAT 
GATGCTAACT GGTTTCTTCA GTTGCTGTCT ACGGACGACC TTTTTGAAAT GACCAGCAAA 
GAGTTTCCTG TAGTAGCTGA AGTTGTCGAA ATATCTCAAG GGAACCACCT GCCCCAAAGT 
ATTTTGCAGC GAGAGAAAAC CATTGTCATC CACAAAAAGT ACCAGGCTTC GAGGATCTTA 
GCTTCAGAAA TTCGCAGCAA TTTCCCTAAA AGACACTTCT TGATCCCTAT TAGCTACAAA 
GGCAAGTTCA AAAGAAGACC ACGGGAGTTC CCCACGGCCT ATGACCTGCA GATAGCTAAG 
AGCAGGAAAG AAACTCTCCA CGTGGTGGCC ACCAAAGCTT TCCATACACT TCACAAGGAG 
CTGTCCCCTG TGTCTGTTGG AGACCAGTTT CTAGTGCATC ATTCAGAGAC CACAGAAGTT 
GTCTTTGAGG GGACAAGAAA AGTAAACGTT CTGACCTGTG AAAAAGTCCT CAATAAGACC 
CGTGAAGATG CACAGCTTCC TCTATACATG GAAGGAGGTT TTGTAGAGGT GATTCATGAT 
AAGAAACAGT ATCAGATTTC CGAGCTCTGT ACCCAGTTTT GCTGGCCCTT CAATGTGAAG 
GTGGCTGTGA GAGATCTCTC CATTAAAGAT GACATTTTGG CAGCTACCCC AGGACTTCAG 
TTAGAGGAGG ACATCACAGA TTCTTATCTA CTTATCAGTG ACTTTGCAAA TCCTGAGGAA 
TGTTGGGAAA TTCCCATGAG CCGCTTGAAT ATGACTGTTC GACTAGTTAA TGGTAGCAGT 
TTGCCTGCAG ATGCAGGGCT ACTTCAAGTC AGGAGTTTTG TAGAAGAGAT TACAGAAGAA 
CAGTATTACA TGATGAGGAG GTACGAAAGT TCTCTATCAC ATCCCCCACC TCGCCCTCCC 
AAACATCCCT CAGCTGAGGA AATGAAGCTC ACCTTGCTCA GCTTAGCAGA AGAGAGAACA 
ATAAATCTGC CCAAGTCTCT CAAGGATAGG ACACCTTGTT GTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361628.1
CDS	266..2053
Translation	MALSLEEFVYSLDLRTLPRVLEIQSGIYFEGSVYEMFGNECCLSTGEVIKITGLKIKKMMAEICEGAIGGCESQKPFELPMNFPGLFKVMADKTPYLSIEEITRTVNIGPSRLGHPCFYHLKDIKLENLIIKQGEPIRFNSVEEINGETLVNCGVVRNHQSHSFTLPLSQEGEFYECEDEHIYTLKEIVEWKIPKNRTRTVKLTDFSNKWDSTNPFPEDFYGTLILKPVYEIQGVLKFQKDIVRILPSLDVEVKDITDSYDANWFLQLLSTDDLFEMTSKEFPVVAEVVEISQGNHLPQSILQREKTIVIHKKYQASRILASEIRSNFPKRHFLIPISYKGKFKRRPREFPTAYDLQIAKSRKETLHVVATKAFHTLHKELSPVSVGDQFLVHHSETTEVVFEGTRKVNVLTCEKVLNKTREDAQLPLYMEGGFVEVIHDKKQYQISELCTQFCWPFNVKVAVRDLSIKDDILAATPGLQLEEDITDSYLLISDFANPEECWEIPMSRLNMTVRLVNGSSLPADAGLLQVRSFVEEITEEQYYMMRRYESSLSHPPPRPPKHPSAEEMKLTLLSLAEERTINLPKSLKDRTPCCC

Target ORF information:

RefSeq Version	NM_001374699.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thymocyte selection associated (Themis), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374699.1

ORF Insert Sequence:

ATGGCTTTAT CTCTGGAAGA ATTTGTCTAC TCCCTTGACC TGAGGACCCT CCCCAGGGTC 
CTAGAAATCC AGTCAGGCAT CTACTTTGAA GGGTCTGTTT ATGAGATGTT TGGCAATGAA 
TGCTGTCTGT CAACAGGAGA AGTGATTAAA ATCACCGGTC TCAAAATTAA GAAGATGATG 
GCTGAAATTT GTGAGGGCGC TATTGGAGGT TGTGAGTCTC AGAAGCCTTT TGAGCTGCCC 
ATGAATTTTC CAGGTCTCTT TAAGGTCATG GCTGATAAAA CTCCATACCT GTCAATTGAA 
GAAATCACAA GAACCGTTAA TATTGGACCC AGCAGACTAG GTCATCCTTG CTTTTATCAC 
CTGAAGGATA TAAAACTAGA GAATCTCATT ATTAAGCAGG GTGAGCCAAT CCGGTTCAAC 
TCAGTTGAAG AGATTAATGG AGAAACGCTG GTGAATTGTG GTGTGGTAAG GAATCATCAG 
AGCCACTCAT TTACTTTGCC ATTGTCACAA GAAGGAGAAT TTTATGAGTG TGAGGATGAA 
CATATCTATA CCCTAAAAGA AATTGTTGAA TGGAAGATTC CCAAGAACAG AACACGGACA 
GTGAAACTTA CAGATTTCTC GAATAAGTGG GACTCAACCA ATCCATTCCC AGAAGACTTT 
TATGGTACAC TGATTCTCAA ACCTGTTTAT GAAATCCAAG GTGTGCTGAA ATTTCAAAAG 
GACATAGTCC GGATTCTCCC AAGTTTAGAC GTTGAAGTCA AAGATATTAC TGATTCTTAT 
GATGCTAACT GGTTTCTTCA GTTGCTGTCT ACGGACGACC TTTTTGAAAT GACCAGCAAA 
GAGTTTCCTG TAGTAGCTGA AGTTGTCGAA ATATCTCAAG GGAACCACCT GCCCCAAAGT 
ATTTTGCAGC GAGAGAAAAC CATTGTCATC CACAAAAAGT ACCAGGCTTC GAGGATCTTA 
GCTTCAGAAA TTCGCAGCAA TTTCCCTAAA AGACACTTCT TGATCCCTAT TAGCTACAAA 
GGCAAGTTCA AAAGAAGACC ACGGGAGTTC CCCACGGCCT ATGACCTGCA GATAGCTAAG 
AGCAGGAAAG AAACTCTCCA CGTGGTGGCC ACCAAAGCTT TCCATACACT TCACAAGGAG 
CTGTCCCCTG TGTCTGTTGG AGACCAGTTT CTAGTGCATC ATTCAGAGAC CACAGAAGTT 
GTCTTTGAGG GGACAAGAAA AGTAAACGTT CTGACCTGTG AAAAAGTCCT CAATAAGACC 
CGTGAAGATG CACAGCTTCC TCTATACATG GAAGGAGGTT TTGTAGAGGT GATTCATGAT 
AAGAAACAGT ATCAGATTTC CGAGCTCTGT ACCCAGTTTT GCTGGCCCTT CAATGTGAAG 
GTGGCTGTGA GAGATCTCTC CATTAAAGAT GACATTTTGG CAGCTACCCC AGGACTTCAG 
TTAGAGGAGG ACATCACAGA TTCTTATCTA CTTATCAGTG ACTTTGCAAA TCCTGAGGAA 
TGTTGGGAAA TTCCCATGAG CCGCTTGAAT ATGACTGTTC GACTAGTTAA TGGTAGCAGT 
TTGCCTGCAG ATGCAGGGCT ACTTCAAGTC AGGAGTTTTG TAGAAGAGAT TACAGAAGAA 
CAGTATTACA TGATGAGGAG GTACGAAAGT TCTCTATCAC ATCCCCCACC TCGCCCTCCC 
AAACATCCCT CAGCTGAGGA AATGAAGCTC ACCTTGCTCA GCTTAGCAGA AGAGAGAACA 
ATAAATCTGC CCAAGTCTCT CAAGGATAGG ACACCTTGTT GTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Grb2-Mediated Recruitment of USP9X to LAT Enhances Themis Stability following Thymic Selection.
Journal of immunology (Baltimore, Md. : 1950)199(8)2758-2766(2017 Oct)
Garreau A,Blaize G,Argenty J,Rouqui? N,Tourd?s A,Wood SA,Saoudi A,Lesourne R

Proteomic Analysis of Regulatory T Cells Reveals the Importance of Themis1 in the Control of Their Suppressive Function.
Molecular & cellular proteomics : MCP16(8)1416-1432(2017 Aug)
Duguet F,Locard-Paulet M,Marcellin M,Chaoui K,Bernard I,Andreoletti O,Lesourne R,Burlet-Schiltz O,Gonzalez de Peredo A,Saoudi A

THEMIS enhances TCR signaling and enables positive selection by selective inhibition of the phosphatase SHP-1.
Nature immunology18(4)433-441(2017 Apr)
Choi S,Warzecha C,Zvezdova E,Lee J,Argenty J,Lesourne R,Aravind L,Love PE

Themis1 enhances T cell receptor signaling during thymocyte development by promoting Vav1 activity and Grb2 stability.
Science signaling9(428)ra51(2016 May)
Zvezdova E,Mikolajczak J,Garreau A,Marcellin M,Rigal L,Lee J,Choi S,Blaize G,Argenty J,Familiades J,Li L,Gonzalez de Peredo A,Burlet-Schiltz O,Love PE,Lesourne R

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Themis cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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FAX

オンラインフォーム