ORF » Species Summary » Mus musculus » Themis cDNA ORF clone

Themis cDNA ORF clone, Mus musculus(house mouse)

  • Gene

  • Clones

  • gRNAs

The following Themis gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Themis cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu18241 NM_178666.6
Latest version!		 Mus musculus thymocyte selection associated (Themis), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu72739 NM_001305663.1
Latest version!		 Mus musculus thymocyte selection associated (Themis), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu43806 XM_006512643.1
Latest version!		 Mus musculus thymocyte selection associated (Themis), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu90618 NM_001374699.1
Latest version!		 Mus musculus thymocyte selection associated (Themis), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    Themis ( NM_178666.6 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_848781.1 Mus musculus thymocyte selection associated (Themis), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OMu18241
    Clone ID Related Accession (Same CDS sequence) NM_178666.6
    Accession Version NM_178666.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1911bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-23
    Organism Mus musculus(house mouse)
    Product protein THEMIS isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB638055.1, AK040594.1, AK169558.1 and AA967480.1. On Mar 26, 2015 this sequence version replaced NM_178666.5. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK088136.1, AK041562.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849388 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCTTTAT CTCTGGAAGA ATTTGTCTAC TCCCTTGACC TGAGGACCCT CCCCAGGGTC 
    CTAGAAATCC AGTCAGGCAT CTACTTTGAA GGGTCTGTTT ATGAGATGTT TGGCAATGAA 
    TGCTGTCTGT CAACAGGAGA AGTGATTAAA ATCACCGGTC TCAAAATTAA GAAGATGATG 
    GCTGAAATTT GTGAGGGCGC TATTGGAGGT TGTGAGTCTC AGAAGCCTTT TGAGCTGCCC 
    ATGAATTTTC CAGGTCTCTT TAAGGTCATG GCTGATAAAA CTCCATACCT GTCAATTGAA 
    GAAATCACAA GAACCGTTAA TATTGGACCC AGCAGACTAG GTCATCCTTG CTTTTATCAC 
    CTGAAGGATA TAAAACTAGA GAATCTCATT ATTAAGCAGG GTGAGCCAAT CCGGTTCAAC 
    TCAGTTGAAG AGATTAATGG AGAAACGCTG GTGAATTGTG GTGTGGTAAG GAATCATCAG 
    AGCCACTCAT TTACTTTGCC ATTGTCACAA GAAGGAGAAT TTTATGAGTG TGAGGATGAA 
    CATATCTATA CCCTAAAAGA AATTGTTGAA TGGAAGATTC CCAAGAACAG AACACGGACA 
    GTGAAACTTA CAGATTTCTC GAATAAGTGG GACTCAACCA ATCCATTCCC AGAAGACTTT 
    TATGGTACAC TGATTCTCAA ACCTGTTTAT GAAATCCAAG GTGTGCTGAA ATTTCAAAAG 
    GACATAGTCC GGATTCTCCC AAGTTTAGAC GTTGAAGTCA AAGATATTAC TGATTCTTAT 
    GATGCTAACT GGTTTCTTCA GTTGCTGTCT ACGGACGACC TTTTTGAAAT GACCAGCAAA 
    GAGTTTCCTG TAGTAGCTGA AGTTGTCGAA ATATCTCAAG GGAACCACCT GCCCCAAAGT 
    ATTTTGCAGC GAGAGAAAAC CATTGTCATC CACAAAAAGT ACCAGGCTTC GAGGATCTTA 
    GCTTCAGAAA TTCGCAGCAA TTTCCCTAAA AGACACTTCT TGATCCCTAT TAGCTACAAA 
    GGCAAGTTCA AAAGAAGACC ACGGGAGTTC CCCACGGCCT ATGACCTGCA GATAGCTAAG 
    AGCAGGAAAG AAACTCTCCA CGTGGTGGCC ACCAAAGCTT TCCATACACT TCACAAGGAG 
    CTGTCCCCTG TGTCTGTTGG AGACCAGTTT CTAGTGCATC ATTCAGAGAC CACAGAAGTT 
    GTCTTTGAGG GGACAAGAAA AGTAAACGTT CTGACCTGTG AAAAAGTCCT CAATAAGACC 
    CGTGAAGATG CACAGCTTCC TCTATACATG GAAGGAGGTT TTGTAGAGGT GATTCATGAT 
    AAGAAACAGT ATCAGATTTC CGAGCTCTGT ACCCAGTTTT GCTGGCCCTT CAATGTGAAG 
    GTGGCTGTGA GAGATCTCTC CATTAAAGAT GACATTTTGG CAGCTACCCC AGGACTTCAG 
    TTAGAGGAGG ACATCACAGA TTCTTATCTA CTTATCAGTG ACTTTGCAAA TCCTGAGGAA 
    TGTTGGGAAA TTCCCATGAG CCGCTTGAAT ATGACTGTTC GACTAGTTAA TGGTAGCAGT 
    TTGCCTGCAG ATGCAGGGCT ACTTCAAGTC AGGAGTTTTG TAGAAGAGAT TACAGAAGAA 
    CAGTATTACA TGATGAGGAG GTACGAAAGT TCTCTATCAC ATCCCCCACC TCGCCCTCCC 
    AAACATCCCT CAGCTGAGGA AATGAAGCTC ACCTTGCTCA GCTTAGCAGA AGAGAGAACA 
    ATAAATCTGC CCAAGTCTCT CAAGAGTCAC CATGTAGACA GACCCAAGAA GCTTCCCTCA 
    GATGAAAGTG GCCAGGATTC AAGAGCTCCA GTTGGTTTTC AGAATGATGT GGCTGATGTG 
    GAGAGACAGA AGAGCAAGCA TGGGCCACTG CAGCCGCAAG CACCACTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_848781.1
    CDS301..2211
    Translation

    Target ORF information:

    RefSeq Version NM_178666.6
    Organism Mus musculus(house mouse)
    Definition Mus musculus thymocyte selection associated (Themis), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_178666.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCTTTAT CTCTGGAAGA ATTTGTCTAC TCCCTTGACC TGAGGACCCT CCCCAGGGTC 
    CTAGAAATCC AGTCAGGCAT CTACTTTGAA GGGTCTGTTT ATGAGATGTT TGGCAATGAA 
    TGCTGTCTGT CAACAGGAGA AGTGATTAAA ATCACCGGTC TCAAAATTAA GAAGATGATG 
    GCTGAAATTT GTGAGGGCGC TATTGGAGGT TGTGAGTCTC AGAAGCCTTT TGAGCTGCCC 
    ATGAATTTTC CAGGTCTCTT TAAGGTCATG GCTGATAAAA CTCCATACCT GTCAATTGAA 
    GAAATCACAA GAACCGTTAA TATTGGACCC AGCAGACTAG GTCATCCTTG CTTTTATCAC 
    CTGAAGGATA TAAAACTAGA GAATCTCATT ATTAAGCAGG GTGAGCCAAT CCGGTTCAAC 
    TCAGTTGAAG AGATTAATGG AGAAACGCTG GTGAATTGTG GTGTGGTAAG GAATCATCAG 
    AGCCACTCAT TTACTTTGCC ATTGTCACAA GAAGGAGAAT TTTATGAGTG TGAGGATGAA 
    CATATCTATA CCCTAAAAGA AATTGTTGAA TGGAAGATTC CCAAGAACAG AACACGGACA 
    GTGAAACTTA CAGATTTCTC GAATAAGTGG GACTCAACCA ATCCATTCCC AGAAGACTTT 
    TATGGTACAC TGATTCTCAA ACCTGTTTAT GAAATCCAAG GTGTGCTGAA ATTTCAAAAG 
    GACATAGTCC GGATTCTCCC AAGTTTAGAC GTTGAAGTCA AAGATATTAC TGATTCTTAT 
    GATGCTAACT GGTTTCTTCA GTTGCTGTCT ACGGACGACC TTTTTGAAAT GACCAGCAAA 
    GAGTTTCCTG TAGTAGCTGA AGTTGTCGAA ATATCTCAAG GGAACCACCT GCCCCAAAGT 
    ATTTTGCAGC GAGAGAAAAC CATTGTCATC CACAAAAAGT ACCAGGCTTC GAGGATCTTA 
    GCTTCAGAAA TTCGCAGCAA TTTCCCTAAA AGACACTTCT TGATCCCTAT TAGCTACAAA 
    GGCAAGTTCA AAAGAAGACC ACGGGAGTTC CCCACGGCCT ATGACCTGCA GATAGCTAAG 
    AGCAGGAAAG AAACTCTCCA CGTGGTGGCC ACCAAAGCTT TCCATACACT TCACAAGGAG 
    CTGTCCCCTG TGTCTGTTGG AGACCAGTTT CTAGTGCATC ATTCAGAGAC CACAGAAGTT 
    GTCTTTGAGG GGACAAGAAA AGTAAACGTT CTGACCTGTG AAAAAGTCCT CAATAAGACC 
    CGTGAAGATG CACAGCTTCC TCTATACATG GAAGGAGGTT TTGTAGAGGT GATTCATGAT 
    AAGAAACAGT ATCAGATTTC CGAGCTCTGT ACCCAGTTTT GCTGGCCCTT CAATGTGAAG 
    GTGGCTGTGA GAGATCTCTC CATTAAAGAT GACATTTTGG CAGCTACCCC AGGACTTCAG 
    TTAGAGGAGG ACATCACAGA TTCTTATCTA CTTATCAGTG ACTTTGCAAA TCCTGAGGAA 
    TGTTGGGAAA TTCCCATGAG CCGCTTGAAT ATGACTGTTC GACTAGTTAA TGGTAGCAGT 
    TTGCCTGCAG ATGCAGGGCT ACTTCAAGTC AGGAGTTTTG TAGAAGAGAT TACAGAAGAA 
    CAGTATTACA TGATGAGGAG GTACGAAAGT TCTCTATCAC ATCCCCCACC TCGCCCTCCC 
    AAACATCCCT CAGCTGAGGA AATGAAGCTC ACCTTGCTCA GCTTAGCAGA AGAGAGAACA 
    ATAAATCTGC CCAAGTCTCT CAAGAGTCAC CATGTAGACA GACCCAAGAA GCTTCCCTCA 
    GATGAAAGTG GCCAGGATTC AAGAGCTCCA GTTGGTTTTC AGAATGATGT GGCTGATGTG 
    GAGAGACAGA AGAGCAAGCA TGGGCCACTG CAGCCGCAAG CACCACTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Themis ( NM_001305663.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001292592.1 Mus musculus thymocyte selection associated (Themis), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OMu72739
    Clone ID Related Accession (Same CDS sequence) NM_001305663.1
    Accession Version NM_001305663.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-23
    Organism Mus musculus(house mouse)
    Product protein THEMIS isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB638055.1, AK040594.1, AK169558.1, AC159472.6 and AC153798.1. Transcript Variant: This variant (2) uses an alternate 5' exon and lacks a 3' coding exon compared to variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). The encoded isoform (2) has a shorter N-terminus and a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK169558.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849383, SAMN00849385 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGCTGATA AAACTCCATA CCTGTCAATT GAAGAAATCA CAAGAACCGT TAATATTGGA 
    CCCAGCAGAC TAGGTCATCC TTGCTTTTAT CACCTGAAGG ATATAAAACT AGAGAATCTC 
    ATTATTAAGC AGGGTGAGCC AATCCGGTTC AACTCAGTTG AAGAGATTAA TGGAGAAACG 
    CTGGTGAATT GTGGTGTGGT AAGGAATCAT CAGAGCCACT CATTTACTTT GCCATTGTCA 
    CAAGAAGGAG AATTTTATGA GTGTGAGGAT GAACATATCT ATACCCTAAA AGAAATTGTT 
    GAATGGAAGA TTCCCAAGAA CAGAACACGG ACAGTGAAAC TTACAGATTT CTCGAATAAG 
    TGGGACTCAA CCAATCCATT CCCAGAAGAC TTTTATGGTA CACTGATTCT CAAACCTGTT 
    TATGAAATCC AAGGTGTGCT GAAATTTCAA AAGGACATAG TCCGGATTCT CCCAAGTTTA 
    GACGTTGAAG TCAAAGATAT TACTGATTCT TATGATGCTA ACTGGTTTCT TCAGTTGCTG 
    TCTACGGACG ACCTTTTTGA AATGACCAGC AAAGAGTTTC CTGTAGTAGC TGAAGTTGTC 
    GAAATATCTC AAGGGAACCA CCTGCCCCAA AGTATTTTGC AGCGAGAGAA AACCATTGTC 
    ATCCACAAAA AGTACCAGGC TTCGAGGATC TTAGCTTCAG AAATTCGCAG CAATTTCCCT 
    AAAAGACACT TCTTGATCCC TATTAGCTAC AAAGGCAAGT TCAAAAGAAG ACCACGGGAG 
    TTCCCCACGG CCTATGACCT GCAGATAGCT AAGAGCAGGA AAGAAACTCT CCACGTGGTG 
    GCCACCAAAG CTTTCCATAC ACTTCACAAG GAGCTGTCCC CTGTGTCTGT TGGAGACCAG 
    TTTCTAGTGC ATCATTCAGA GACCACAGAA GTTGTCTTTG AGGGGACAAG AAAAGTAAAC 
    GTTCTGACCT GTGAAAAAGT CCTCAATAAG ACCCGTGAAG ATGCACAGCT TCCTCTATAC 
    ATGGAAGGAG GTTTTGTAGA GGTGATTCAT GATAAGAAAC AGTATCAGAT TTCCGAGCTC 
    TGTACCCAGT TTTGCTGGCC CTTCAATGTG AAGGTGGCTG TGAGAGATCT CTCCATTAAA 
    GATGACATTT TGGCAGCTAC CCCAGGACTT CAGTTAGAGG AGGACATCAC AGATTCTTAT 
    CTACTTATCA GTGACTTTGC AAATCCTGAG GAATGTTGGG AAATTCCCAT GAGCCGCTTG 
    AATATGACTG TTCGACTAGT TAATGGTAGC AGTTTGCCTG CAGATGCAGG GCTACTTCAA 
    GTCAGGAGTT TTGTAGAAGA GATTACAGAA GAACAGTATT ACATGATGAG GAGGTACGAA 
    AGTTCTCTAT CACATCCCCC ACCTCGCCCT CCCAAACATC CCTCAGCTGA GGAAATGAAG 
    CTCACCTTGC TCAGCTTAGC AGAAGAGAGA ACAATAAATC TGCCCAAGTC TCTCAAGAAC 
    TCTTCAAAAC TGAAAAACAT CAAAAATAAC AAGACGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001292592.1
    CDS730..2268
    Translation

    Target ORF information:

    RefSeq Version NM_001305663.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus thymocyte selection associated (Themis), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001305663.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGCTGATA AAACTCCATA CCTGTCAATT GAAGAAATCA CAAGAACCGT TAATATTGGA 
    CCCAGCAGAC TAGGTCATCC TTGCTTTTAT CACCTGAAGG ATATAAAACT AGAGAATCTC 
    ATTATTAAGC AGGGTGAGCC AATCCGGTTC AACTCAGTTG AAGAGATTAA TGGAGAAACG 
    CTGGTGAATT GTGGTGTGGT AAGGAATCAT CAGAGCCACT CATTTACTTT GCCATTGTCA 
    CAAGAAGGAG AATTTTATGA GTGTGAGGAT GAACATATCT ATACCCTAAA AGAAATTGTT 
    GAATGGAAGA TTCCCAAGAA CAGAACACGG ACAGTGAAAC TTACAGATTT CTCGAATAAG 
    TGGGACTCAA CCAATCCATT CCCAGAAGAC TTTTATGGTA CACTGATTCT CAAACCTGTT 
    TATGAAATCC AAGGTGTGCT GAAATTTCAA AAGGACATAG TCCGGATTCT CCCAAGTTTA 
    GACGTTGAAG TCAAAGATAT TACTGATTCT TATGATGCTA ACTGGTTTCT TCAGTTGCTG 
    TCTACGGACG ACCTTTTTGA AATGACCAGC AAAGAGTTTC CTGTAGTAGC TGAAGTTGTC 
    GAAATATCTC AAGGGAACCA CCTGCCCCAA AGTATTTTGC AGCGAGAGAA AACCATTGTC 
    ATCCACAAAA AGTACCAGGC TTCGAGGATC TTAGCTTCAG AAATTCGCAG CAATTTCCCT 
    AAAAGACACT TCTTGATCCC TATTAGCTAC AAAGGCAAGT TCAAAAGAAG ACCACGGGAG 
    TTCCCCACGG CCTATGACCT GCAGATAGCT AAGAGCAGGA AAGAAACTCT CCACGTGGTG 
    GCCACCAAAG CTTTCCATAC ACTTCACAAG GAGCTGTCCC CTGTGTCTGT TGGAGACCAG 
    TTTCTAGTGC ATCATTCAGA GACCACAGAA GTTGTCTTTG AGGGGACAAG AAAAGTAAAC 
    GTTCTGACCT GTGAAAAAGT CCTCAATAAG ACCCGTGAAG ATGCACAGCT TCCTCTATAC 
    ATGGAAGGAG GTTTTGTAGA GGTGATTCAT GATAAGAAAC AGTATCAGAT TTCCGAGCTC 
    TGTACCCAGT TTTGCTGGCC CTTCAATGTG AAGGTGGCTG TGAGAGATCT CTCCATTAAA 
    GATGACATTT TGGCAGCTAC CCCAGGACTT CAGTTAGAGG AGGACATCAC AGATTCTTAT 
    CTACTTATCA GTGACTTTGC AAATCCTGAG GAATGTTGGG AAATTCCCAT GAGCCGCTTG 
    AATATGACTG TTCGACTAGT TAATGGTAGC AGTTTGCCTG CAGATGCAGG GCTACTTCAA 
    GTCAGGAGTT TTGTAGAAGA GATTACAGAA GAACAGTATT ACATGATGAG GAGGTACGAA 
    AGTTCTCTAT CACATCCCCC ACCTCGCCCT CCCAAACATC CCTCAGCTGA GGAAATGAAG 
    CTCACCTTGC TCAGCTTAGC AGAAGAGAGA ACAATAAATC TGCCCAAGTC TCTCAAGAAC 
    TCTTCAAAAC TGAAAAACAT CAAAAATAAC AAGACGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Themis ( XM_006512643.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512706.1 Mus musculus thymocyte selection associated (Themis), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OMu43806
    Clone ID Related Accession (Same CDS sequence) XM_006512643.1
    Accession Version XM_006512643.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1644bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein THEMIS isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCTGATA AAACTCCATA CCTGTCAATT GAAGAAATCA CAAGAACCGT TAATATTGGA 
    CCCAGCAGAC TAGGTCATCC TTGCTTTTAT CACCTGAAGG ATATAAAACT AGAGAATCTC 
    ATTATTAAGC AGGGTGAGCC AATCCGGTTC AACTCAGTTG AAGAGATTAA TGGAGAAACG 
    CTGGTGAATT GTGGTGTGGT AAGGAATCAT CAGAGCCACT CATTTACTTT GCCATTGTCA 
    CAAGAAGGAG AATTTTATGA GTGTGAGGAT GAACATATCT ATACCCTAAA AGAAATTGTT 
    GAATGGAAGA TTCCCAAGAA CAGAACACGG ACAGTGAAAC TTACAGATTT CTCGAATAAG 
    TGGGACTCAA CCAATCCATT CCCAGAAGAC TTTTATGGTA CACTGATTCT CAAACCTGTT 
    TATGAAATCC AAGGTGTGCT GAAATTTCAA AAGGACATAG TCCGGATTCT CCCAAGTTTA 
    GACGTTGAAG TCAAAGATAT TACTGATTCT TATGATGCTA ACTGGTTTCT TCAGTTGCTG 
    TCTACGGACG ACCTTTTTGA AATGACCAGC AAAGAGTTTC CTGTAGTAGC TGAAGTTGTC 
    GAAATATCTC AAGGGAACCA CCTGCCCCAA AGTATTTTGC AGCGAGAGAA AACCATTGTC 
    ATCCACAAAA AGTACCAGGC TTCGAGGATC TTAGCTTCAG AAATTCGCAG CAATTTCCCT 
    AAAAGACACT TCTTGATCCC TATTAGCTAC AAAGGCAAGT TCAAAAGAAG ACCACGGGAG 
    TTCCCCACGG CCTATGACCT GCAGATAGCT AAGAGCAGGA AAGAAACTCT CCACGTGGTG 
    GCCACCAAAG CTTTCCATAC ACTTCACAAG GAGCTGTCCC CTGTGTCTGT TGGAGACCAG 
    TTTCTAGTGC ATCATTCAGA GACCACAGAA GTTGTCTTTG AGGGGACAAG AAAAGTAAAC 
    GTTCTGACCT GTGAAAAAGT CCTCAATAAG ACCCGTGAAG ATGCACAGCT TCCTCTATAC 
    ATGGAAGGAG GTTTTGTAGA GGTGATTCAT GATAAGAAAC AGTATCAGAT TTCCGAGCTC 
    TGTACCCAGT TTTGCTGGCC CTTCAATGTG AAGGTGGCTG TGAGAGATCT CTCCATTAAA 
    GATGACATTT TGGCAGCTAC CCCAGGACTT CAGTTAGAGG AGGACATCAC AGATTCTTAT 
    CTACTTATCA GTGACTTTGC AAATCCTGAG GAATGTTGGG AAATTCCCAT GAGCCGCTTG 
    AATATGACTG TTCGACTAGT TAATGGTAGC AGTTTGCCTG CAGATGCAGG GCTACTTCAA 
    GTCAGGAGTT TTGTAGAAGA GATTACAGAA GAACAGTATT ACATGATGAG GAGGTACGAA 
    AGTTCTCTAT CACATCCCCC ACCTCGCCCT CCCAAACATC CCTCAGCTGA GGAAATGAAG 
    CTCACCTTGC TCAGCTTAGC AGAAGAGAGA ACAATAAATC TGCCCAAGTC TCTCAAGAGT 
    CACCATGTAG ACAGACCCAA GAAGCTTCCC TCAGATGAAA GTGGCCAGGA TTCAAGAGCT 
    CCAGTTGGTT TTCAGAATGA TGTGGCTGAT GTGGAGAGAC AGAAGAGCAA GCATGGGCCA 
    CTGCAGCCGC AAGCACCACT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512706.1
    CDS119..1762
    Translation

    Target ORF information:

    RefSeq Version XM_006512643.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus thymocyte selection associated (Themis), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006512643.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCTGATA AAACTCCATA CCTGTCAATT GAAGAAATCA CAAGAACCGT TAATATTGGA 
    CCCAGCAGAC TAGGTCATCC TTGCTTTTAT CACCTGAAGG ATATAAAACT AGAGAATCTC 
    ATTATTAAGC AGGGTGAGCC AATCCGGTTC AACTCAGTTG AAGAGATTAA TGGAGAAACG 
    CTGGTGAATT GTGGTGTGGT AAGGAATCAT CAGAGCCACT CATTTACTTT GCCATTGTCA 
    CAAGAAGGAG AATTTTATGA GTGTGAGGAT GAACATATCT ATACCCTAAA AGAAATTGTT 
    GAATGGAAGA TTCCCAAGAA CAGAACACGG ACAGTGAAAC TTACAGATTT CTCGAATAAG 
    TGGGACTCAA CCAATCCATT CCCAGAAGAC TTTTATGGTA CACTGATTCT CAAACCTGTT 
    TATGAAATCC AAGGTGTGCT GAAATTTCAA AAGGACATAG TCCGGATTCT CCCAAGTTTA 
    GACGTTGAAG TCAAAGATAT TACTGATTCT TATGATGCTA ACTGGTTTCT TCAGTTGCTG 
    TCTACGGACG ACCTTTTTGA AATGACCAGC AAAGAGTTTC CTGTAGTAGC TGAAGTTGTC 
    GAAATATCTC AAGGGAACCA CCTGCCCCAA AGTATTTTGC AGCGAGAGAA AACCATTGTC 
    ATCCACAAAA AGTACCAGGC TTCGAGGATC TTAGCTTCAG AAATTCGCAG CAATTTCCCT 
    AAAAGACACT TCTTGATCCC TATTAGCTAC AAAGGCAAGT TCAAAAGAAG ACCACGGGAG 
    TTCCCCACGG CCTATGACCT GCAGATAGCT AAGAGCAGGA AAGAAACTCT CCACGTGGTG 
    GCCACCAAAG CTTTCCATAC ACTTCACAAG GAGCTGTCCC CTGTGTCTGT TGGAGACCAG 
    TTTCTAGTGC ATCATTCAGA GACCACAGAA GTTGTCTTTG AGGGGACAAG AAAAGTAAAC 
    GTTCTGACCT GTGAAAAAGT CCTCAATAAG ACCCGTGAAG ATGCACAGCT TCCTCTATAC 
    ATGGAAGGAG GTTTTGTAGA GGTGATTCAT GATAAGAAAC AGTATCAGAT TTCCGAGCTC 
    TGTACCCAGT TTTGCTGGCC CTTCAATGTG AAGGTGGCTG TGAGAGATCT CTCCATTAAA 
    GATGACATTT TGGCAGCTAC CCCAGGACTT CAGTTAGAGG AGGACATCAC AGATTCTTAT 
    CTACTTATCA GTGACTTTGC AAATCCTGAG GAATGTTGGG AAATTCCCAT GAGCCGCTTG 
    AATATGACTG TTCGACTAGT TAATGGTAGC AGTTTGCCTG CAGATGCAGG GCTACTTCAA 
    GTCAGGAGTT TTGTAGAAGA GATTACAGAA GAACAGTATT ACATGATGAG GAGGTACGAA 
    AGTTCTCTAT CACATCCCCC ACCTCGCCCT CCCAAACATC CCTCAGCTGA GGAAATGAAG 
    CTCACCTTGC TCAGCTTAGC AGAAGAGAGA ACAATAAATC TGCCCAAGTC TCTCAAGAGT 
    CACCATGTAG ACAGACCCAA GAAGCTTCCC TCAGATGAAA GTGGCCAGGA TTCAAGAGCT 
    CCAGTTGGTT TTCAGAATGA TGTGGCTGAT GTGGAGAGAC AGAAGAGCAA GCATGGGCCA 
    CTGCAGCCGC AAGCACCACT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Themis ( NM_001374699.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001361628.1 Mus musculus thymocyte selection associated (Themis), transcript variant 4, mRNA.
    Price & Availability↑
    CloneID OMu90618
    Clone ID Related Accession (Same CDS sequence) NM_001374699.1
    Accession Version NM_001374699.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1788bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-24
    Organism Mus musculus(house mouse)
    Product protein THEMIS isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC152983.2 and AC159472.6. On Oct 9, 2019 this sequence version replaced XM_030244987.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK138701.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCTTTAT CTCTGGAAGA ATTTGTCTAC TCCCTTGACC TGAGGACCCT CCCCAGGGTC 
    CTAGAAATCC AGTCAGGCAT CTACTTTGAA GGGTCTGTTT ATGAGATGTT TGGCAATGAA 
    TGCTGTCTGT CAACAGGAGA AGTGATTAAA ATCACCGGTC TCAAAATTAA GAAGATGATG 
    GCTGAAATTT GTGAGGGCGC TATTGGAGGT TGTGAGTCTC AGAAGCCTTT TGAGCTGCCC 
    ATGAATTTTC CAGGTCTCTT TAAGGTCATG GCTGATAAAA CTCCATACCT GTCAATTGAA 
    GAAATCACAA GAACCGTTAA TATTGGACCC AGCAGACTAG GTCATCCTTG CTTTTATCAC 
    CTGAAGGATA TAAAACTAGA GAATCTCATT ATTAAGCAGG GTGAGCCAAT CCGGTTCAAC 
    TCAGTTGAAG AGATTAATGG AGAAACGCTG GTGAATTGTG GTGTGGTAAG GAATCATCAG 
    AGCCACTCAT TTACTTTGCC ATTGTCACAA GAAGGAGAAT TTTATGAGTG TGAGGATGAA 
    CATATCTATA CCCTAAAAGA AATTGTTGAA TGGAAGATTC CCAAGAACAG AACACGGACA 
    GTGAAACTTA CAGATTTCTC GAATAAGTGG GACTCAACCA ATCCATTCCC AGAAGACTTT 
    TATGGTACAC TGATTCTCAA ACCTGTTTAT GAAATCCAAG GTGTGCTGAA ATTTCAAAAG 
    GACATAGTCC GGATTCTCCC AAGTTTAGAC GTTGAAGTCA AAGATATTAC TGATTCTTAT 
    GATGCTAACT GGTTTCTTCA GTTGCTGTCT ACGGACGACC TTTTTGAAAT GACCAGCAAA 
    GAGTTTCCTG TAGTAGCTGA AGTTGTCGAA ATATCTCAAG GGAACCACCT GCCCCAAAGT 
    ATTTTGCAGC GAGAGAAAAC CATTGTCATC CACAAAAAGT ACCAGGCTTC GAGGATCTTA 
    GCTTCAGAAA TTCGCAGCAA TTTCCCTAAA AGACACTTCT TGATCCCTAT TAGCTACAAA 
    GGCAAGTTCA AAAGAAGACC ACGGGAGTTC CCCACGGCCT ATGACCTGCA GATAGCTAAG 
    AGCAGGAAAG AAACTCTCCA CGTGGTGGCC ACCAAAGCTT TCCATACACT TCACAAGGAG 
    CTGTCCCCTG TGTCTGTTGG AGACCAGTTT CTAGTGCATC ATTCAGAGAC CACAGAAGTT 
    GTCTTTGAGG GGACAAGAAA AGTAAACGTT CTGACCTGTG AAAAAGTCCT CAATAAGACC 
    CGTGAAGATG CACAGCTTCC TCTATACATG GAAGGAGGTT TTGTAGAGGT GATTCATGAT 
    AAGAAACAGT ATCAGATTTC CGAGCTCTGT ACCCAGTTTT GCTGGCCCTT CAATGTGAAG 
    GTGGCTGTGA GAGATCTCTC CATTAAAGAT GACATTTTGG CAGCTACCCC AGGACTTCAG 
    TTAGAGGAGG ACATCACAGA TTCTTATCTA CTTATCAGTG ACTTTGCAAA TCCTGAGGAA 
    TGTTGGGAAA TTCCCATGAG CCGCTTGAAT ATGACTGTTC GACTAGTTAA TGGTAGCAGT 
    TTGCCTGCAG ATGCAGGGCT ACTTCAAGTC AGGAGTTTTG TAGAAGAGAT TACAGAAGAA 
    CAGTATTACA TGATGAGGAG GTACGAAAGT TCTCTATCAC ATCCCCCACC TCGCCCTCCC 
    AAACATCCCT CAGCTGAGGA AATGAAGCTC ACCTTGCTCA GCTTAGCAGA AGAGAGAACA 
    ATAAATCTGC CCAAGTCTCT CAAGGATAGG ACACCTTGTT GTTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001361628.1
    CDS266..2053
    Translation

    Target ORF information:

    RefSeq Version NM_001374699.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus thymocyte selection associated (Themis), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001374699.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCTTTAT CTCTGGAAGA ATTTGTCTAC TCCCTTGACC TGAGGACCCT CCCCAGGGTC 
    CTAGAAATCC AGTCAGGCAT CTACTTTGAA GGGTCTGTTT ATGAGATGTT TGGCAATGAA 
    TGCTGTCTGT CAACAGGAGA AGTGATTAAA ATCACCGGTC TCAAAATTAA GAAGATGATG 
    GCTGAAATTT GTGAGGGCGC TATTGGAGGT TGTGAGTCTC AGAAGCCTTT TGAGCTGCCC 
    ATGAATTTTC CAGGTCTCTT TAAGGTCATG GCTGATAAAA CTCCATACCT GTCAATTGAA 
    GAAATCACAA GAACCGTTAA TATTGGACCC AGCAGACTAG GTCATCCTTG CTTTTATCAC 
    CTGAAGGATA TAAAACTAGA GAATCTCATT ATTAAGCAGG GTGAGCCAAT CCGGTTCAAC 
    TCAGTTGAAG AGATTAATGG AGAAACGCTG GTGAATTGTG GTGTGGTAAG GAATCATCAG 
    AGCCACTCAT TTACTTTGCC ATTGTCACAA GAAGGAGAAT TTTATGAGTG TGAGGATGAA 
    CATATCTATA CCCTAAAAGA AATTGTTGAA TGGAAGATTC CCAAGAACAG AACACGGACA 
    GTGAAACTTA CAGATTTCTC GAATAAGTGG GACTCAACCA ATCCATTCCC AGAAGACTTT 
    TATGGTACAC TGATTCTCAA ACCTGTTTAT GAAATCCAAG GTGTGCTGAA ATTTCAAAAG 
    GACATAGTCC GGATTCTCCC AAGTTTAGAC GTTGAAGTCA AAGATATTAC TGATTCTTAT 
    GATGCTAACT GGTTTCTTCA GTTGCTGTCT ACGGACGACC TTTTTGAAAT GACCAGCAAA 
    GAGTTTCCTG TAGTAGCTGA AGTTGTCGAA ATATCTCAAG GGAACCACCT GCCCCAAAGT 
    ATTTTGCAGC GAGAGAAAAC CATTGTCATC CACAAAAAGT ACCAGGCTTC GAGGATCTTA 
    GCTTCAGAAA TTCGCAGCAA TTTCCCTAAA AGACACTTCT TGATCCCTAT TAGCTACAAA 
    GGCAAGTTCA AAAGAAGACC ACGGGAGTTC CCCACGGCCT ATGACCTGCA GATAGCTAAG 
    AGCAGGAAAG AAACTCTCCA CGTGGTGGCC ACCAAAGCTT TCCATACACT TCACAAGGAG 
    CTGTCCCCTG TGTCTGTTGG AGACCAGTTT CTAGTGCATC ATTCAGAGAC CACAGAAGTT 
    GTCTTTGAGG GGACAAGAAA AGTAAACGTT CTGACCTGTG AAAAAGTCCT CAATAAGACC 
    CGTGAAGATG CACAGCTTCC TCTATACATG GAAGGAGGTT TTGTAGAGGT GATTCATGAT 
    AAGAAACAGT ATCAGATTTC CGAGCTCTGT ACCCAGTTTT GCTGGCCCTT CAATGTGAAG 
    GTGGCTGTGA GAGATCTCTC CATTAAAGAT GACATTTTGG CAGCTACCCC AGGACTTCAG 
    TTAGAGGAGG ACATCACAGA TTCTTATCTA CTTATCAGTG ACTTTGCAAA TCCTGAGGAA 
    TGTTGGGAAA TTCCCATGAG CCGCTTGAAT ATGACTGTTC GACTAGTTAA TGGTAGCAGT 
    TTGCCTGCAG ATGCAGGGCT ACTTCAAGTC AGGAGTTTTG TAGAAGAGAT TACAGAAGAA 
    CAGTATTACA TGATGAGGAG GTACGAAAGT TCTCTATCAC ATCCCCCACC TCGCCCTCCC 
    AAACATCCCT CAGCTGAGGA AATGAAGCTC ACCTTGCTCA GCTTAGCAGA AGAGAGAACA 
    ATAAATCTGC CCAAGTCTCT CAAGGATAGG ACACCTTGTT GTTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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