THEMIS gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001318531.1	NP_001305460.1	protein THEMIS isoform 4
XM_024446434.1	XP_024302202.1	protein THEMIS isoform X4
NM_001164687.1	NP_001158159.1	protein THEMIS isoform 3
XM_024446433.1	XP_024302201.1	protein THEMIS isoform X1
XM_017010848.1	XP_016866337.1	protein THEMIS isoform X3
XM_011535813.2	XP_011534115.1	protein THEMIS isoform X1
XM_011535816.1	XP_011534118.1	protein THEMIS isoform X4
NM_001010923.2	NP_001010923.1	protein THEMIS isoform 2
XM_011535814.2	XP_011534116.1	protein THEMIS isoform X2
XM_017010849.1	XP_016866338.1	protein THEMIS isoform X5
NM_001164685.1	NP_001158157.1	protein THEMIS isoform 1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03624	NM_001164685.1 Latest version!	Homo sapiens thymocyte selection associated (THEMIS), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu18249	NM_001164687.1 Latest version!	Homo sapiens thymocyte selection associated (THEMIS), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu32256	NM_001010923.2 Latest version!	Homo sapiens thymocyte selection associated (THEMIS), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu40804	NM_001318531.1 Latest version!	Homo sapiens thymocyte selection associated (THEMIS), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu40804	XM_011535816.1 Latest version!	Homo sapiens thymocyte selection associated (THEMIS), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu40804	XM_024446434.1 Latest version!	Homo sapiens thymocyte selection associated (THEMIS), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu79835	XM_011535813.2 Latest version!	Homo sapiens thymocyte selection associated (THEMIS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu79835	XM_024446433.1 Latest version!	Homo sapiens thymocyte selection associated (THEMIS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu79836	XM_011535814.2 Latest version!	Homo sapiens thymocyte selection associated (THEMIS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu96593	XM_017010849.1 Latest version!	Homo sapiens thymocyte selection associated (THEMIS), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu96592	XM_017010848.1 Latest version!	Homo sapiens thymocyte selection associated (THEMIS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

THEMIS ( NM_001164685.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158157.1 Homo sapiens thymocyte selection associated (THEMIS), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03624
Clone ID Related Accession (Same CDS sequence)	NM_001164685.1
Accession Version	NM_001164685.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	protein THEMIS isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX956657.1, BC144034.1, BC130516.1, BC043608.1 and BG461112.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144034.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA2144333 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCATTAT CACTGGAAGA ATTCGTCCAC TCCCTTGACC TCAGGACCCT ACCCAGGGTT 
CTAGAAATCC AGGCAGGCAT CTATCTTGAA GGCTCTATTT ATGAAATGTT TGGAAATGAA 
TGCTGTTTTT CAACAGGAGA AGTGATTAAA ATTACTGGTC TCAAAGTTAA GAAGATCATA 
GCTGAAATTT GTGAGCAGAT TGAAGGTTGT GAGTCTCTAC AGCCATTTGA ACTGCCTATG 
AATTTTCCAG GTCTTTTTAA GATTGTGGCT GATAAAACTC CATACCTTAC TATGGAAGAA 
ATCACAAGGA CCATTCATAT TGGACCAAGT AGACTAGGGC ATCCTTGCTT CTATCATCAG 
AAGGATATAA AACTAGAGAA CCTCATCATA AAGCAGGGTG AGCAAATCAT GCTCAACTCA 
GTTGAAGAGA TTGATGGAGA AATAATGGTG AGCTGTGCAG TAGCAAGGAA TCATCAAACT 
CACTCATTTA ATTTGCCTTT GTCACAAGAA GGAGAATTCT ACGAGTGTGA AGATGAACGT 
ATTTACACTC TAAAGGAGAT TGTTGAATGG AAGATTCCTA AGAACAGAAC AAGAACTGTA 
AACCTTACAG ATTTTTCAAA TAAGTGGGAC TCAACGAATC CATTTCCTAA AGACTTTTAT 
GGTACCCTGA TTCTCAAGCC TGTTTATGAA ATTCAAGGTG TGATGAAATT TCGAAAAGAT 
ATAATCCGCA TCCTCCCCAG TCTAGATGTC GAAGTCAAAG ACATCACTGA TTCTTACGAT 
GCTAACTGGT TTCTTCAGCT GTTATCAACA GAAGATCTTT TTGAAATGAC TAGTAAAGAG 
TTCCCCATAG TGACTGAAGT CATAGAAGCA CCTGAAGGAA ACCACCTGCC CCAAAGCATT 
TTACAGCCTG GGAAAACCAT TGTGATCCAC AAAAAGTACC AGGCATCAAG AATCTTAGCT 
TCAGAAATTA GAAGCAATTT TCCTAAAAGA CACTTCTTGA TCCCCACTAG CTATAAAGGC 
AAGTTCAAGC GGCGACCGAG GGAGTTCCCA ACGGCCTATG ACCTAGAGAT CGCTAAGAGT 
GAAAAGGAGC CTCTTCACGT GGTGGCCACC AAAGCGTTTC ATTCCCCTCA TGACAAGCTG 
TCATCCGTAT CTGTTGGGGA CCAGTTTCTG GTGCATCAGT CAGAGACGAC TGAAGTCCTC 
TGTGAGGGAA TAAAAAAAGT GGTGAATGTT CTGGCCTGTG AAAAAATCCT CAAAAAGTCC 
TATGAGGCTG CGCTGCTCCC TTTGTACATG GAAGGAGGTT TTGTAGAGGT GATTCATGAT 
AAGAAACAGT ACCCGATTTC TGAGCTCTGT AAACAGTTCC GTTTGCCCTT CAATGTGAAG 
GTGTCTGTCA GGGATCTTTC CATTGAAGAG GACGTGTTGG CTGCCACACC AGGACTGCAG 
TTGGAGGAGG ACATTACAGA CTCTTACCTA CTCATAAGTG ACTTTGCCAA CCCCACGGAG 
TGCTGGGAAA TTCCTGTGGG CCGCTTGAAT ATGACTGTTC AGTTAGTTAG TAATTTCTCT 
AGGGATGCAG AACCATTTCT AGTCAGGACT CTGGTAGAAG AGATCACTGA AGAGCAATAT 
TACATGATGC GGAGATATGA AAGCTCAGCC TCACATCCCC CACCTCGCCC TCCGAAACAC 
CCCTCAGTAG AGGAAACAAA GTTAACCCTG CTAACCTTAG CAGAAGAAAG GACGGTAGAC 
CTGCCCAAGT CTCCCAAGGC TGGAGTGCAA TGGCGTGATC TTGGCTCGCT GCAACCTCTG 
CCTCCCGGGT TCAAGCAATT CTCTGCCTCA GCCTCCCACG TAGCTGGGAT TACAGGCACC 
CCCCACCACG TCCAGCGTCA TCACGTAGAC ATAACCAAGA AACTTCACCC AAATCAAGCT 
GGCCTGGATT CAAAAGTACT GATTGGTAGT CAGAATGATT TGGTGGATGA AGAGAAAGAA 
AGGAGCAACC GTGGGGCCAC AGCAATAGCA GAAACATTCA AAAATGAAAA ACATCAAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158157.1
CDS	150..2192
Translation	MALSLEEFVHSLDLRTLPRVLEIQAGIYLEGSIYEMFGNECCFSTGEVIKITGLKVKKIIAEICEQIEGCESLQPFELPMNFPGLFKIVADKTPYLTMEEITRTIHIGPSRLGHPCFYHQKDIKLENLIIKQGEQIMLNSVEEIDGEIMVSCAVARNHQTHSFNLPLSQEGEFYECEDERIYTLKEIVEWKIPKNRTRTVNLTDFSNKWDSTNPFPKDFYGTLILKPVYEIQGVMKFRKDIIRILPSLDVEVKDITDSYDANWFLQLLSTEDLFEMTSKEFPIVTEVIEAPEGNHLPQSILQPGKTIVIHKKYQASRILASEIRSNFPKRHFLIPTSYKGKFKRRPREFPTAYDLEIAKSEKEPLHVVATKAFHSPHDKLSSVSVGDQFLVHQSETTEVLCEGIKKVVNVLACEKILKKSYEAALLPLYMEGGFVEVIHDKKQYPISELCKQFRLPFNVKVSVRDLSIEEDVLAATPGLQLEEDITDSYLLISDFANPTECWEIPVGRLNMTVQLVSNFSRDAEPFLVRTLVEEITEEQYYMMRRYESSASHPPPRPPKHPSVEETKLTLLTLAEERTVDLPKSPKAGVQWRDLGSLQPLPPGFKQFSASASHVAGITGTPHHVQRHHVDITKKLHPNQAGLDSKVLIGSQNDLVDEEKERSNRGATAIAETFKNEKHQK

Target ORF information:

RefSeq Version	NM_001164685.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thymocyte selection associated (THEMIS), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164685.1

ORF Insert Sequence:

ATGGCATTAT CACTGGAAGA ATTCGTCCAC TCCCTTGACC TCAGGACCCT ACCCAGGGTT 
CTAGAAATCC AGGCAGGCAT CTATCTTGAA GGCTCTATTT ATGAAATGTT TGGAAATGAA 
TGCTGTTTTT CAACAGGAGA AGTGATTAAA ATTACTGGTC TCAAAGTTAA GAAGATCATA 
GCTGAAATTT GTGAGCAGAT TGAAGGTTGT GAGTCTCTAC AGCCATTTGA ACTGCCTATG 
AATTTTCCAG GTCTTTTTAA GATTGTGGCT GATAAAACTC CATACCTTAC TATGGAAGAA 
ATCACAAGGA CCATTCATAT TGGACCAAGT AGACTAGGGC ATCCTTGCTT CTATCATCAG 
AAGGATATAA AACTAGAGAA CCTCATCATA AAGCAGGGTG AGCAAATCAT GCTCAACTCA 
GTTGAAGAGA TTGATGGAGA AATAATGGTG AGCTGTGCAG TAGCAAGGAA TCATCAAACT 
CACTCATTTA ATTTGCCTTT GTCACAAGAA GGAGAATTCT ACGAGTGTGA AGATGAACGT 
ATTTACACTC TAAAGGAGAT TGTTGAATGG AAGATTCCTA AGAACAGAAC AAGAACTGTA 
AACCTTACAG ATTTTTCAAA TAAGTGGGAC TCAACGAATC CATTTCCTAA AGACTTTTAT 
GGTACCCTGA TTCTCAAGCC TGTTTATGAA ATTCAAGGTG TGATGAAATT TCGAAAAGAT 
ATAATCCGCA TCCTCCCCAG TCTAGATGTC GAAGTCAAAG ACATCACTGA TTCTTACGAT 
GCTAACTGGT TTCTTCAGCT GTTATCAACA GAAGATCTTT TTGAAATGAC TAGTAAAGAG 
TTCCCCATAG TGACTGAAGT CATAGAAGCA CCTGAAGGAA ACCACCTGCC CCAAAGCATT 
TTACAGCCTG GGAAAACCAT TGTGATCCAC AAAAAGTACC AGGCATCAAG AATCTTAGCT 
TCAGAAATTA GAAGCAATTT TCCTAAAAGA CACTTCTTGA TCCCCACTAG CTATAAAGGC 
AAGTTCAAGC GGCGACCGAG GGAGTTCCCA ACGGCCTATG ACCTAGAGAT CGCTAAGAGT 
GAAAAGGAGC CTCTTCACGT GGTGGCCACC AAAGCGTTTC ATTCCCCTCA TGACAAGCTG 
TCATCCGTAT CTGTTGGGGA CCAGTTTCTG GTGCATCAGT CAGAGACGAC TGAAGTCCTC 
TGTGAGGGAA TAAAAAAAGT GGTGAATGTT CTGGCCTGTG AAAAAATCCT CAAAAAGTCC 
TATGAGGCTG CGCTGCTCCC TTTGTACATG GAAGGAGGTT TTGTAGAGGT GATTCATGAT 
AAGAAACAGT ACCCGATTTC TGAGCTCTGT AAACAGTTCC GTTTGCCCTT CAATGTGAAG 
GTGTCTGTCA GGGATCTTTC CATTGAAGAG GACGTGTTGG CTGCCACACC AGGACTGCAG 
TTGGAGGAGG ACATTACAGA CTCTTACCTA CTCATAAGTG ACTTTGCCAA CCCCACGGAG 
TGCTGGGAAA TTCCTGTGGG CCGCTTGAAT ATGACTGTTC AGTTAGTTAG TAATTTCTCT 
AGGGATGCAG AACCATTTCT AGTCAGGACT CTGGTAGAAG AGATCACTGA AGAGCAATAT 
TACATGATGC GGAGATATGA AAGCTCAGCC TCACATCCCC CACCTCGCCC TCCGAAACAC 
CCCTCAGTAG AGGAAACAAA GTTAACCCTG CTAACCTTAG CAGAAGAAAG GACGGTAGAC 
CTGCCCAAGT CTCCCAAGGC TGGAGTGCAA TGGCGTGATC TTGGCTCGCT GCAACCTCTG 
CCTCCCGGGT TCAAGCAATT CTCTGCCTCA GCCTCCCACG TAGCTGGGAT TACAGGCACC 
CCCCACCACG TCCAGCGTCA TCACGTAGAC ATAACCAAGA AACTTCACCC AAATCAAGCT 
GGCCTGGATT CAAAAGTACT GATTGGTAGT CAGAATGATT TGGTGGATGA AGAGAAAGAA 
AGGAGCAACC GTGGGGCCAC AGCAATAGCA GAAACATTCA AAAATGAAAA ACATCAAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THEMIS ( NM_001164687.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158159.1 Homo sapiens thymocyte selection associated (THEMIS), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu18249
Clone ID Related Accession (Same CDS sequence)	NM_001164687.1
Accession Version	NM_001164687.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	protein THEMIS isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX956657.1, AK124031.1, DA818250.1, BC130516.1, BC043608.1 and BG461112.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, uses a downstream translational start codon, and lacks an alternate in-frame exon in the central coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK124031.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTGGAA ATGAATGCTG TTTTTCAACA GGAGAAGTGA TTAAAATTAC TGGTCTCAAA 
GTTAAGAAGA TCATAGCTGA AATTTGTGAG CAGATTGAAG GTTGTGAGTC TCTACAGCCA 
TTTGAACTGC CTATGAATTT TCCAGGTCTT TTTAAGATTG TGGCTGATAA AACTCCATAC 
CTTACTATGG AAGAAATCAC AAGGACCATT CATATTGGAC CAAGTAGACT AGGGCATCCT 
TGCTTCTATC ATCAGAAGGA TATAAAACTA GAGAACCTCA TCATAAAGCA GGGTGAGCAA 
ATCATGCTCA ACTCAGTTGA AGAGATTGAT GGAGAAATAA TGGTGAGCTG TGCAGTAGCA 
AGGAATCATC AAACTCACTC ATTTAATTTG CCTTTGTCAC AAGAAGGAGA ATTCTACGAG 
TGTGAAGATG AACGTATTTA CACTCTAAAG GAGATTGTTG AATGGAAGAT TCCTAAGAAC 
AGAACAAGAA CTGTAAACCT TACAGATTTT TCAAATAAGT GGGACTCAAC GAATCCATTT 
CCTAAAGACT TTTATGGTAC CCTGATTCTC AAGCCTGTTT ATGAAATTCA AGGTGTGATG 
AAATTTCGAA AAGATATAAT CCGCATCCTC CCCAGTCTAG ATGTCGAAGT CAAAGACATC 
ACTGATTCTT ACGATGCTAA CTGGTTTCTT CAGCTGTTAT CAACAGAAGA TCTTTTTGAA 
ATGACTAGTA AAGAGTTCCC CATAGTGACT GAAGTCATAG AAGCACCTGA AGGAAACCAC 
CTGCCCCAAA GCATTTTACA GCCTGGGAAA ACCATTGTGA TCCACAAAAA GTACCAGGCA 
TCAAGAATCT TAGCTTCAGA AATTAGAAGC AATTTTCCTA AAAGACACTT CTTGATCCCC 
ACTAGCTATA AAGGCAAGTT CAAGCGGCGA CCGAGGGAGT TCCCAACGGC CTATGACCTA 
GAGATCGCTA AGAGTGAAAA GGAGCCTCTT CACGTGGTGG CCACCAAAGC GTTTCATTCC 
CCTCATGACA AGCTGTCATC CGTATCTGTT GGGGACCAGT TTCTGGTGCA TCAGTCAGAG 
ACGACTGAAG TCCTCTGTGA GGGAATAAAA AAAGTGGTGA ATGTTCTGGC CTGTGAAAAA 
ATCCTCAAAA AGTCCTATGA GGCTGCGCTG CTCCCTTTGT ACATGGAAGG AGGTTTTGTA 
GAGGTGATTC ATGATAAGAA ACAGTACCCG ATTTCTGAGC TCTGTAAACA GTTCCGTTTG 
CCCTTCAATG TGAAGGTGTC TGTCAGGGAT CTTTCCATTG AAGAGGACGT GTTGGCTGCC 
ACACCAGGAC TGCAGTTGGA GGAGGACATT ACAGACTCTT ACCTACTCAT AAGTGACTTT 
GCCAACCCCA CGGAGTGCTG GGAAATTCCT GTGGGCCGCT TGAATATGAC TGTTCAGTTA 
GTTAGTAATT TCTCTAGGGA TGCAGAACCA TTTCTAGTCA GGACTCTGGT AGAAGAGATC 
ACTGAAGAGC AATATTACAT GATGCGGAGA TATGAAAGCT CAGCCTCACA TCCCCCACCT 
CGCCCTCCGA AACACCCCTC AGTAGAGGAA ACAAAGTTAA CCCTGCTAAC CTTAGCAGAA 
GAAAGGACGG TAGACCTGCC CAAGTCTCCC AAGCGTCATC ACGTAGACAT AACCAAGAAA 
CTTCACCCAA ATCAAGCTGG CCTGGATTCA AAAGTACTGA TTGGTAGTCA GAATGATTTG 
GTGGATGAAG AGAAAGAAAG GAGCAACCGT GGGGCCACAG CAATAGCAGA AACATTCAAA 
AATGAAAAAC ATCAAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158159.1
CDS	219..2039
Translation	MFGNECCFSTGEVIKITGLKVKKIIAEICEQIEGCESLQPFELPMNFPGLFKIVADKTPYLTMEEITRTIHIGPSRLGHPCFYHQKDIKLENLIIKQGEQIMLNSVEEIDGEIMVSCAVARNHQTHSFNLPLSQEGEFYECEDERIYTLKEIVEWKIPKNRTRTVNLTDFSNKWDSTNPFPKDFYGTLILKPVYEIQGVMKFRKDIIRILPSLDVEVKDITDSYDANWFLQLLSTEDLFEMTSKEFPIVTEVIEAPEGNHLPQSILQPGKTIVIHKKYQASRILASEIRSNFPKRHFLIPTSYKGKFKRRPREFPTAYDLEIAKSEKEPLHVVATKAFHSPHDKLSSVSVGDQFLVHQSETTEVLCEGIKKVVNVLACEKILKKSYEAALLPLYMEGGFVEVIHDKKQYPISELCKQFRLPFNVKVSVRDLSIEEDVLAATPGLQLEEDITDSYLLISDFANPTECWEIPVGRLNMTVQLVSNFSRDAEPFLVRTLVEEITEEQYYMMRRYESSASHPPPRPPKHPSVEETKLTLLTLAEERTVDLPKSPKRHHVDITKKLHPNQAGLDSKVLIGSQNDLVDEEKERSNRGATAIAETFKNEKHQK

Target ORF information:

RefSeq Version	NM_001164687.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thymocyte selection associated (THEMIS), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164687.1

ORF Insert Sequence:

ATGTTTGGAA ATGAATGCTG TTTTTCAACA GGAGAAGTGA TTAAAATTAC TGGTCTCAAA 
GTTAAGAAGA TCATAGCTGA AATTTGTGAG CAGATTGAAG GTTGTGAGTC TCTACAGCCA 
TTTGAACTGC CTATGAATTT TCCAGGTCTT TTTAAGATTG TGGCTGATAA AACTCCATAC 
CTTACTATGG AAGAAATCAC AAGGACCATT CATATTGGAC CAAGTAGACT AGGGCATCCT 
TGCTTCTATC ATCAGAAGGA TATAAAACTA GAGAACCTCA TCATAAAGCA GGGTGAGCAA 
ATCATGCTCA ACTCAGTTGA AGAGATTGAT GGAGAAATAA TGGTGAGCTG TGCAGTAGCA 
AGGAATCATC AAACTCACTC ATTTAATTTG CCTTTGTCAC AAGAAGGAGA ATTCTACGAG 
TGTGAAGATG AACGTATTTA CACTCTAAAG GAGATTGTTG AATGGAAGAT TCCTAAGAAC 
AGAACAAGAA CTGTAAACCT TACAGATTTT TCAAATAAGT GGGACTCAAC GAATCCATTT 
CCTAAAGACT TTTATGGTAC CCTGATTCTC AAGCCTGTTT ATGAAATTCA AGGTGTGATG 
AAATTTCGAA AAGATATAAT CCGCATCCTC CCCAGTCTAG ATGTCGAAGT CAAAGACATC 
ACTGATTCTT ACGATGCTAA CTGGTTTCTT CAGCTGTTAT CAACAGAAGA TCTTTTTGAA 
ATGACTAGTA AAGAGTTCCC CATAGTGACT GAAGTCATAG AAGCACCTGA AGGAAACCAC 
CTGCCCCAAA GCATTTTACA GCCTGGGAAA ACCATTGTGA TCCACAAAAA GTACCAGGCA 
TCAAGAATCT TAGCTTCAGA AATTAGAAGC AATTTTCCTA AAAGACACTT CTTGATCCCC 
ACTAGCTATA AAGGCAAGTT CAAGCGGCGA CCGAGGGAGT TCCCAACGGC CTATGACCTA 
GAGATCGCTA AGAGTGAAAA GGAGCCTCTT CACGTGGTGG CCACCAAAGC GTTTCATTCC 
CCTCATGACA AGCTGTCATC CGTATCTGTT GGGGACCAGT TTCTGGTGCA TCAGTCAGAG 
ACGACTGAAG TCCTCTGTGA GGGAATAAAA AAAGTGGTGA ATGTTCTGGC CTGTGAAAAA 
ATCCTCAAAA AGTCCTATGA GGCTGCGCTG CTCCCTTTGT ACATGGAAGG AGGTTTTGTA 
GAGGTGATTC ATGATAAGAA ACAGTACCCG ATTTCTGAGC TCTGTAAACA GTTCCGTTTG 
CCCTTCAATG TGAAGGTGTC TGTCAGGGAT CTTTCCATTG AAGAGGACGT GTTGGCTGCC 
ACACCAGGAC TGCAGTTGGA GGAGGACATT ACAGACTCTT ACCTACTCAT AAGTGACTTT 
GCCAACCCCA CGGAGTGCTG GGAAATTCCT GTGGGCCGCT TGAATATGAC TGTTCAGTTA 
GTTAGTAATT TCTCTAGGGA TGCAGAACCA TTTCTAGTCA GGACTCTGGT AGAAGAGATC 
ACTGAAGAGC AATATTACAT GATGCGGAGA TATGAAAGCT CAGCCTCACA TCCCCCACCT 
CGCCCTCCGA AACACCCCTC AGTAGAGGAA ACAAAGTTAA CCCTGCTAAC CTTAGCAGAA 
GAAAGGACGG TAGACCTGCC CAAGTCTCCC AAGCGTCATC ACGTAGACAT AACCAAGAAA 
CTTCACCCAA ATCAAGCTGG CCTGGATTCA AAAGTACTGA TTGGTAGTCA GAATGATTTG 
GTGGATGAAG AGAAAGAAAG GAGCAACCGT GGGGCCACAG CAATAGCAGA AACATTCAAA 
AATGAAAAAC ATCAAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THEMIS ( NM_001010923.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001010923.1 Homo sapiens thymocyte selection associated (THEMIS), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu32256
Clone ID Related Accession (Same CDS sequence)	NM_001010923.2
Accession Version	NM_001010923.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	protein THEMIS isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX956657.1, BC130516.1, BC043608.1 and BG461112.1. On Sep 10, 2009 this sequence version replaced NM_001010923.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC130516.1, SRR1803615.171666.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCATTAT CACTGGAAGA ATTCGTCCAC TCCCTTGACC TCAGGACCCT ACCCAGGGTT 
CTAGAAATCC AGGCAGGCAT CTATCTTGAA GGCTCTATTT ATGAAATGTT TGGAAATGAA 
TGCTGTTTTT CAACAGGAGA AGTGATTAAA ATTACTGGTC TCAAAGTTAA GAAGATCATA 
GCTGAAATTT GTGAGCAGAT TGAAGGTTGT GAGTCTCTAC AGCCATTTGA ACTGCCTATG 
AATTTTCCAG GTCTTTTTAA GATTGTGGCT GATAAAACTC CATACCTTAC TATGGAAGAA 
ATCACAAGGA CCATTCATAT TGGACCAAGT AGACTAGGGC ATCCTTGCTT CTATCATCAG 
AAGGATATAA AACTAGAGAA CCTCATCATA AAGCAGGGTG AGCAAATCAT GCTCAACTCA 
GTTGAAGAGA TTGATGGAGA AATAATGGTG AGCTGTGCAG TAGCAAGGAA TCATCAAACT 
CACTCATTTA ATTTGCCTTT GTCACAAGAA GGAGAATTCT ACGAGTGTGA AGATGAACGT 
ATTTACACTC TAAAGGAGAT TGTTGAATGG AAGATTCCTA AGAACAGAAC AAGAACTGTA 
AACCTTACAG ATTTTTCAAA TAAGTGGGAC TCAACGAATC CATTTCCTAA AGACTTTTAT 
GGTACCCTGA TTCTCAAGCC TGTTTATGAA ATTCAAGGTG TGATGAAATT TCGAAAAGAT 
ATAATCCGCA TCCTCCCCAG TCTAGATGTC GAAGTCAAAG ACATCACTGA TTCTTACGAT 
GCTAACTGGT TTCTTCAGCT GTTATCAACA GAAGATCTTT TTGAAATGAC TAGTAAAGAG 
TTCCCCATAG TGACTGAAGT CATAGAAGCA CCTGAAGGAA ACCACCTGCC CCAAAGCATT 
TTACAGCCTG GGAAAACCAT TGTGATCCAC AAAAAGTACC AGGCATCAAG AATCTTAGCT 
TCAGAAATTA GAAGCAATTT TCCTAAAAGA CACTTCTTGA TCCCCACTAG CTATAAAGGC 
AAGTTCAAGC GGCGACCGAG GGAGTTCCCA ACGGCCTATG ACCTAGAGAT CGCTAAGAGT 
GAAAAGGAGC CTCTTCACGT GGTGGCCACC AAAGCGTTTC ATTCCCCTCA TGACAAGCTG 
TCATCCGTAT CTGTTGGGGA CCAGTTTCTG GTGCATCAGT CAGAGACGAC TGAAGTCCTC 
TGTGAGGGAA TAAAAAAAGT GGTGAATGTT CTGGCCTGTG AAAAAATCCT CAAAAAGTCC 
TATGAGGCTG CGCTGCTCCC TTTGTACATG GAAGGAGGTT TTGTAGAGGT GATTCATGAT 
AAGAAACAGT ACCCGATTTC TGAGCTCTGT AAACAGTTCC GTTTGCCCTT CAATGTGAAG 
GTGTCTGTCA GGGATCTTTC CATTGAAGAG GACGTGTTGG CTGCCACACC AGGACTGCAG 
TTGGAGGAGG ACATTACAGA CTCTTACCTA CTCATAAGTG ACTTTGCCAA CCCCACGGAG 
TGCTGGGAAA TTCCTGTGGG CCGCTTGAAT ATGACTGTTC AGTTAGTTAG TAATTTCTCT 
AGGGATGCAG AACCATTTCT AGTCAGGACT CTGGTAGAAG AGATCACTGA AGAGCAATAT 
TACATGATGC GGAGATATGA AAGCTCAGCC TCACATCCCC CACCTCGCCC TCCGAAACAC 
CCCTCAGTAG AGGAAACAAA GTTAACCCTG CTAACCTTAG CAGAAGAAAG GACGGTAGAC 
CTGCCCAAGT CTCCCAAGCG TCATCACGTA GACATAACCA AGAAACTTCA CCCAAATCAA 
GCTGGCCTGG ATTCAAAAGT ACTGATTGGT AGTCAGAATG ATTTGGTGGA TGAAGAGAAA 
GAAAGGAGCA ACCGTGGGGC CACAGCAATA GCAGAAACAT TCAAAAATGA AAAACATCAA 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001010923.1
CDS	150..2075
Translation	MALSLEEFVHSLDLRTLPRVLEIQAGIYLEGSIYEMFGNECCFSTGEVIKITGLKVKKIIAEICEQIEGCESLQPFELPMNFPGLFKIVADKTPYLTMEEITRTIHIGPSRLGHPCFYHQKDIKLENLIIKQGEQIMLNSVEEIDGEIMVSCAVARNHQTHSFNLPLSQEGEFYECEDERIYTLKEIVEWKIPKNRTRTVNLTDFSNKWDSTNPFPKDFYGTLILKPVYEIQGVMKFRKDIIRILPSLDVEVKDITDSYDANWFLQLLSTEDLFEMTSKEFPIVTEVIEAPEGNHLPQSILQPGKTIVIHKKYQASRILASEIRSNFPKRHFLIPTSYKGKFKRRPREFPTAYDLEIAKSEKEPLHVVATKAFHSPHDKLSSVSVGDQFLVHQSETTEVLCEGIKKVVNVLACEKILKKSYEAALLPLYMEGGFVEVIHDKKQYPISELCKQFRLPFNVKVSVRDLSIEEDVLAATPGLQLEEDITDSYLLISDFANPTECWEIPVGRLNMTVQLVSNFSRDAEPFLVRTLVEEITEEQYYMMRRYESSASHPPPRPPKHPSVEETKLTLLTLAEERTVDLPKSPKRHHVDITKKLHPNQAGLDSKVLIGSQNDLVDEEKERSNRGATAIAETFKNEKHQK

Target ORF information:

RefSeq Version	NM_001010923.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thymocyte selection associated (THEMIS), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001010923.2

ORF Insert Sequence:

ATGGCATTAT CACTGGAAGA ATTCGTCCAC TCCCTTGACC TCAGGACCCT ACCCAGGGTT 
CTAGAAATCC AGGCAGGCAT CTATCTTGAA GGCTCTATTT ATGAAATGTT TGGAAATGAA 
TGCTGTTTTT CAACAGGAGA AGTGATTAAA ATTACTGGTC TCAAAGTTAA GAAGATCATA 
GCTGAAATTT GTGAGCAGAT TGAAGGTTGT GAGTCTCTAC AGCCATTTGA ACTGCCTATG 
AATTTTCCAG GTCTTTTTAA GATTGTGGCT GATAAAACTC CATACCTTAC TATGGAAGAA 
ATCACAAGGA CCATTCATAT TGGACCAAGT AGACTAGGGC ATCCTTGCTT CTATCATCAG 
AAGGATATAA AACTAGAGAA CCTCATCATA AAGCAGGGTG AGCAAATCAT GCTCAACTCA 
GTTGAAGAGA TTGATGGAGA AATAATGGTG AGCTGTGCAG TAGCAAGGAA TCATCAAACT 
CACTCATTTA ATTTGCCTTT GTCACAAGAA GGAGAATTCT ACGAGTGTGA AGATGAACGT 
ATTTACACTC TAAAGGAGAT TGTTGAATGG AAGATTCCTA AGAACAGAAC AAGAACTGTA 
AACCTTACAG ATTTTTCAAA TAAGTGGGAC TCAACGAATC CATTTCCTAA AGACTTTTAT 
GGTACCCTGA TTCTCAAGCC TGTTTATGAA ATTCAAGGTG TGATGAAATT TCGAAAAGAT 
ATAATCCGCA TCCTCCCCAG TCTAGATGTC GAAGTCAAAG ACATCACTGA TTCTTACGAT 
GCTAACTGGT TTCTTCAGCT GTTATCAACA GAAGATCTTT TTGAAATGAC TAGTAAAGAG 
TTCCCCATAG TGACTGAAGT CATAGAAGCA CCTGAAGGAA ACCACCTGCC CCAAAGCATT 
TTACAGCCTG GGAAAACCAT TGTGATCCAC AAAAAGTACC AGGCATCAAG AATCTTAGCT 
TCAGAAATTA GAAGCAATTT TCCTAAAAGA CACTTCTTGA TCCCCACTAG CTATAAAGGC 
AAGTTCAAGC GGCGACCGAG GGAGTTCCCA ACGGCCTATG ACCTAGAGAT CGCTAAGAGT 
GAAAAGGAGC CTCTTCACGT GGTGGCCACC AAAGCGTTTC ATTCCCCTCA TGACAAGCTG 
TCATCCGTAT CTGTTGGGGA CCAGTTTCTG GTGCATCAGT CAGAGACGAC TGAAGTCCTC 
TGTGAGGGAA TAAAAAAAGT GGTGAATGTT CTGGCCTGTG AAAAAATCCT CAAAAAGTCC 
TATGAGGCTG CGCTGCTCCC TTTGTACATG GAAGGAGGTT TTGTAGAGGT GATTCATGAT 
AAGAAACAGT ACCCGATTTC TGAGCTCTGT AAACAGTTCC GTTTGCCCTT CAATGTGAAG 
GTGTCTGTCA GGGATCTTTC CATTGAAGAG GACGTGTTGG CTGCCACACC AGGACTGCAG 
TTGGAGGAGG ACATTACAGA CTCTTACCTA CTCATAAGTG ACTTTGCCAA CCCCACGGAG 
TGCTGGGAAA TTCCTGTGGG CCGCTTGAAT ATGACTGTTC AGTTAGTTAG TAATTTCTCT 
AGGGATGCAG AACCATTTCT AGTCAGGACT CTGGTAGAAG AGATCACTGA AGAGCAATAT 
TACATGATGC GGAGATATGA AAGCTCAGCC TCACATCCCC CACCTCGCCC TCCGAAACAC 
CCCTCAGTAG AGGAAACAAA GTTAACCCTG CTAACCTTAG CAGAAGAAAG GACGGTAGAC 
CTGCCCAAGT CTCCCAAGCG TCATCACGTA GACATAACCA AGAAACTTCA CCCAAATCAA 
GCTGGCCTGG ATTCAAAAGT ACTGATTGGT AGTCAGAATG ATTTGGTGGA TGAAGAGAAA 
GAAAGGAGCA ACCGTGGGGC CACAGCAATA GCAGAAACAT TCAAAAATGA AAAACATCAA 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THEMIS ( NM_001318531.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305460.1 Homo sapiens thymocyte selection associated (THEMIS), transcript variant 4, mRNA.

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CloneID	OHu40804
Clone ID Related Accession (Same CDS sequence)	NM_001318531.1 , XM_024446434.1 , XM_011535816.1
Accession Version	NM_001318531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	protein THEMIS isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC130516.1, AK128377.1, AL365224.8, BC043608.1 and BG461112.1. On or before Jan 8, 2016 this sequence version replaced XM_011535815.1, XM_011546485.1. Transcript Variant: This variant (4) contains multiple differences in the 5' region, initiates translation at a downstream start codon, and lacks an alternate in-frame exon in the central coding region, compared to variant 1.. The encoded isoform (4) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK128377.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAAA TCACAAGGAC CATTCATATT GGACCAAGTA GACTAGGGCA TCCTTGCTTC 
TATCATCAGA AGGATATAAA ACTAGAGAAC CTCATCATAA AGCAGGGTGA GCAAATCATG 
CTCAACTCAG TTGAAGAGAT TGATGGAGAA ATAATGGTGA GCTGTGCAGT AGCAAGGAAT 
CATCAAACTC ACTCATTTAA TTTGCCTTTG TCACAAGAAG GAGAATTCTA CGAGTGTGAA 
GATGAACGTA TTTACACTCT AAAGGAGATT GTTGAATGGA AGATTCCTAA GAACAGAACA 
AGAACTGTAA ACCTTACAGA TTTTTCAAAT AAGTGGGACT CAACGAATCC ATTTCCTAAA 
GACTTTTATG GTACCCTGAT TCTCAAGCCT GTTTATGAAA TTCAAGGTGT GATGAAATTT 
CGAAAAGATA TAATCCGCAT CCTCCCCAGT CTAGATGTCG AAGTCAAAGA CATCACTGAT 
TCTTACGATG CTAACTGGTT TCTTCAGCTG TTATCAACAG AAGATCTTTT TGAAATGACT 
AGTAAAGAGT TCCCCATAGT GACTGAAGTC ATAGAAGCAC CTGAAGGAAA CCACCTGCCC 
CAAAGCATTT TACAGCCTGG GAAAACCATT GTGATCCACA AAAAGTACCA GGCATCAAGA 
ATCTTAGCTT CAGAAATTAG AAGCAATTTT CCTAAAAGAC ACTTCTTGAT CCCCACTAGC 
TATAAAGGCA AGTTCAAGCG GCGACCGAGG GAGTTCCCAA CGGCCTATGA CCTAGAGATC 
GCTAAGAGTG AAAAGGAGCC TCTTCACGTG GTGGCCACCA AAGCGTTTCA TTCCCCTCAT 
GACAAGCTGT CATCCGTATC TGTTGGGGAC CAGTTTCTGG TGCATCAGTC AGAGACGACT 
GAAGTCCTCT GTGAGGGAAT AAAAAAAGTG GTGAATGTTC TGGCCTGTGA AAAAATCCTC 
AAAAAGTCCT ATGAGGCTGC GCTGCTCCCT TTGTACATGG AAGGAGGTTT TGTAGAGGTG 
ATTCATGATA AGAAACAGTA CCCGATTTCT GAGCTCTGTA AACAGTTCCG TTTGCCCTTC 
AATGTGAAGG TGTCTGTCAG GGATCTTTCC ATTGAAGAGG ACGTGTTGGC TGCCACACCA 
GGACTGCAGT TGGAGGAGGA CATTACAGAC TCTTACCTAC TCATAAGTGA CTTTGCCAAC 
CCCACGGAGT GCTGGGAAAT TCCTGTGGGC CGCTTGAATA TGACTGTTCA GTTAGTTAGT 
AATTTCTCTA GGGATGCAGA ACCATTTCTA GTCAGGACTC TGGTAGAAGA GATCACTGAA 
GAGCAATATT ACATGATGCG GAGATATGAA AGCTCAGCCT CACATCCCCC ACCTCGCCCT 
CCGAAACACC CCTCAGTAGA GGAAACAAAG TTAACCCTGC TAACCTTAGC AGAAGAAAGG 
ACGGTAGACC TGCCCAAGTC TCCCAAGCGT CATCACGTAG ACATAACCAA GAAACTTCAC 
CCAAATCAAG CTGGCCTGGA TTCAAAAGTA CTGATTGGTA GTCAGAATGA TTTGGTGGAT 
GAAGAGAAAG AAAGGAGCAA CCGTGGGGCC ACAGCAATAG CAGAAACATT CAAAAATGAA 
AAACATCAAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305460.1
CDS	506..2140
Translation	MEEITRTIHIGPSRLGHPCFYHQKDIKLENLIIKQGEQIMLNSVEEIDGEIMVSCAVARNHQTHSFNLPLSQEGEFYECEDERIYTLKEIVEWKIPKNRTRTVNLTDFSNKWDSTNPFPKDFYGTLILKPVYEIQGVMKFRKDIIRILPSLDVEVKDITDSYDANWFLQLLSTEDLFEMTSKEFPIVTEVIEAPEGNHLPQSILQPGKTIVIHKKYQASRILASEIRSNFPKRHFLIPTSYKGKFKRRPREFPTAYDLEIAKSEKEPLHVVATKAFHSPHDKLSSVSVGDQFLVHQSETTEVLCEGIKKVVNVLACEKILKKSYEAALLPLYMEGGFVEVIHDKKQYPISELCKQFRLPFNVKVSVRDLSIEEDVLAATPGLQLEEDITDSYLLISDFANPTECWEIPVGRLNMTVQLVSNFSRDAEPFLVRTLVEEITEEQYYMMRRYESSASHPPPRPPKHPSVEETKLTLLTLAEERTVDLPKSPKRHHVDITKKLHPNQAGLDSKVLIGSQNDLVDEEKERSNRGATAIAETFKNEKHQK

Target ORF information:

RefSeq Version	NM_001318531.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thymocyte selection associated (THEMIS), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318531.1

ORF Insert Sequence:

ATGGAAGAAA TCACAAGGAC CATTCATATT GGACCAAGTA GACTAGGGCA TCCTTGCTTC 
TATCATCAGA AGGATATAAA ACTAGAGAAC CTCATCATAA AGCAGGGTGA GCAAATCATG 
CTCAACTCAG TTGAAGAGAT TGATGGAGAA ATAATGGTGA GCTGTGCAGT AGCAAGGAAT 
CATCAAACTC ACTCATTTAA TTTGCCTTTG TCACAAGAAG GAGAATTCTA CGAGTGTGAA 
GATGAACGTA TTTACACTCT AAAGGAGATT GTTGAATGGA AGATTCCTAA GAACAGAACA 
AGAACTGTAA ACCTTACAGA TTTTTCAAAT AAGTGGGACT CAACGAATCC ATTTCCTAAA 
GACTTTTATG GTACCCTGAT TCTCAAGCCT GTTTATGAAA TTCAAGGTGT GATGAAATTT 
CGAAAAGATA TAATCCGCAT CCTCCCCAGT CTAGATGTCG AAGTCAAAGA CATCACTGAT 
TCTTACGATG CTAACTGGTT TCTTCAGCTG TTATCAACAG AAGATCTTTT TGAAATGACT 
AGTAAAGAGT TCCCCATAGT GACTGAAGTC ATAGAAGCAC CTGAAGGAAA CCACCTGCCC 
CAAAGCATTT TACAGCCTGG GAAAACCATT GTGATCCACA AAAAGTACCA GGCATCAAGA 
ATCTTAGCTT CAGAAATTAG AAGCAATTTT CCTAAAAGAC ACTTCTTGAT CCCCACTAGC 
TATAAAGGCA AGTTCAAGCG GCGACCGAGG GAGTTCCCAA CGGCCTATGA CCTAGAGATC 
GCTAAGAGTG AAAAGGAGCC TCTTCACGTG GTGGCCACCA AAGCGTTTCA TTCCCCTCAT 
GACAAGCTGT CATCCGTATC TGTTGGGGAC CAGTTTCTGG TGCATCAGTC AGAGACGACT 
GAAGTCCTCT GTGAGGGAAT AAAAAAAGTG GTGAATGTTC TGGCCTGTGA AAAAATCCTC 
AAAAAGTCCT ATGAGGCTGC GCTGCTCCCT TTGTACATGG AAGGAGGTTT TGTAGAGGTG 
ATTCATGATA AGAAACAGTA CCCGATTTCT GAGCTCTGTA AACAGTTCCG TTTGCCCTTC 
AATGTGAAGG TGTCTGTCAG GGATCTTTCC ATTGAAGAGG ACGTGTTGGC TGCCACACCA 
GGACTGCAGT TGGAGGAGGA CATTACAGAC TCTTACCTAC TCATAAGTGA CTTTGCCAAC 
CCCACGGAGT GCTGGGAAAT TCCTGTGGGC CGCTTGAATA TGACTGTTCA GTTAGTTAGT 
AATTTCTCTA GGGATGCAGA ACCATTTCTA GTCAGGACTC TGGTAGAAGA GATCACTGAA 
GAGCAATATT ACATGATGCG GAGATATGAA AGCTCAGCCT CACATCCCCC ACCTCGCCCT 
CCGAAACACC CCTCAGTAGA GGAAACAAAG TTAACCCTGC TAACCTTAGC AGAAGAAAGG 
ACGGTAGACC TGCCCAAGTC TCCCAAGCGT CATCACGTAG ACATAACCAA GAAACTTCAC 
CCAAATCAAG CTGGCCTGGA TTCAAAAGTA CTGATTGGTA GTCAGAATGA TTTGGTGGAT 
GAAGAGAAAG AAAGGAGCAA CCGTGGGGCC ACAGCAATAG CAGAAACATT CAAAAATGAA 
AAACATCAAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THEMIS ( XM_024446434.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024302202.1 Homo sapiens thymocyte selection associated (THEMIS), transcript variant X4, mRNA.

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CloneID	OHu40804
Clone ID Related Accession (Same CDS sequence)	NM_001318531.1 , XM_024446434.1 , XM_011535816.1
Accession Version	XM_024446434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein THEMIS isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA TCACAAGGAC CATTCATATT GGACCAAGTA GACTAGGGCA TCCTTGCTTC 
TATCATCAGA AGGATATAAA ACTAGAGAAC CTCATCATAA AGCAGGGTGA GCAAATCATG 
CTCAACTCAG TTGAAGAGAT TGATGGAGAA ATAATGGTGA GCTGTGCAGT AGCAAGGAAT 
CATCAAACTC ACTCATTTAA TTTGCCTTTG TCACAAGAAG GAGAATTCTA CGAGTGTGAA 
GATGAACGTA TTTACACTCT AAAGGAGATT GTTGAATGGA AGATTCCTAA GAACAGAACA 
AGAACTGTAA ACCTTACAGA TTTTTCAAAT AAGTGGGACT CAACGAATCC ATTTCCTAAA 
GACTTTTATG GTACCCTGAT TCTCAAGCCT GTTTATGAAA TTCAAGGTGT GATGAAATTT 
CGAAAAGATA TAATCCGCAT CCTCCCCAGT CTAGATGTCG AAGTCAAAGA CATCACTGAT 
TCTTACGATG CTAACTGGTT TCTTCAGCTG TTATCAACAG AAGATCTTTT TGAAATGACT 
AGTAAAGAGT TCCCCATAGT GACTGAAGTC ATAGAAGCAC CTGAAGGAAA CCACCTGCCC 
CAAAGCATTT TACAGCCTGG GAAAACCATT GTGATCCACA AAAAGTACCA GGCATCAAGA 
ATCTTAGCTT CAGAAATTAG AAGCAATTTT CCTAAAAGAC ACTTCTTGAT CCCCACTAGC 
TATAAAGGCA AGTTCAAGCG GCGACCGAGG GAGTTCCCAA CGGCCTATGA CCTAGAGATC 
GCTAAGAGTG AAAAGGAGCC TCTTCACGTG GTGGCCACCA AAGCGTTTCA TTCCCCTCAT 
GACAAGCTGT CATCCGTATC TGTTGGGGAC CAGTTTCTGG TGCATCAGTC AGAGACGACT 
GAAGTCCTCT GTGAGGGAAT AAAAAAAGTG GTGAATGTTC TGGCCTGTGA AAAAATCCTC 
AAAAAGTCCT ATGAGGCTGC GCTGCTCCCT TTGTACATGG AAGGAGGTTT TGTAGAGGTG 
ATTCATGATA AGAAACAGTA CCCGATTTCT GAGCTCTGTA AACAGTTCCG TTTGCCCTTC 
AATGTGAAGG TGTCTGTCAG GGATCTTTCC ATTGAAGAGG ACGTGTTGGC TGCCACACCA 
GGACTGCAGT TGGAGGAGGA CATTACAGAC TCTTACCTAC TCATAAGTGA CTTTGCCAAC 
CCCACGGAGT GCTGGGAAAT TCCTGTGGGC CGCTTGAATA TGACTGTTCA GTTAGTTAGT 
AATTTCTCTA GGGATGCAGA ACCATTTCTA GTCAGGACTC TGGTAGAAGA GATCACTGAA 
GAGCAATATT ACATGATGCG GAGATATGAA AGCTCAGCCT CACATCCCCC ACCTCGCCCT 
CCGAAACACC CCTCAGTAGA GGAAACAAAG TTAACCCTGC TAACCTTAGC AGAAGAAAGG 
ACGGTAGACC TGCCCAAGTC TCCCAAGCGT CATCACGTAG ACATAACCAA GAAACTTCAC 
CCAAATCAAG CTGGCCTGGA TTCAAAAGTA CTGATTGGTA GTCAGAATGA TTTGGTGGAT 
GAAGAGAAAG AAAGGAGCAA CCGTGGGGCC ACAGCAATAG CAGAAACATT CAAAAATGAA 
AAACATCAAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024302202.1
CDS	784..2418
Translation	MEEITRTIHIGPSRLGHPCFYHQKDIKLENLIIKQGEQIMLNSVEEIDGEIMVSCAVARNHQTHSFNLPLSQEGEFYECEDERIYTLKEIVEWKIPKNRTRTVNLTDFSNKWDSTNPFPKDFYGTLILKPVYEIQGVMKFRKDIIRILPSLDVEVKDITDSYDANWFLQLLSTEDLFEMTSKEFPIVTEVIEAPEGNHLPQSILQPGKTIVIHKKYQASRILASEIRSNFPKRHFLIPTSYKGKFKRRPREFPTAYDLEIAKSEKEPLHVVATKAFHSPHDKLSSVSVGDQFLVHQSETTEVLCEGIKKVVNVLACEKILKKSYEAALLPLYMEGGFVEVIHDKKQYPISELCKQFRLPFNVKVSVRDLSIEEDVLAATPGLQLEEDITDSYLLISDFANPTECWEIPVGRLNMTVQLVSNFSRDAEPFLVRTLVEEITEEQYYMMRRYESSASHPPPRPPKHPSVEETKLTLLTLAEERTVDLPKSPKRHHVDITKKLHPNQAGLDSKVLIGSQNDLVDEEKERSNRGATAIAETFKNEKHQK

Target ORF information:

RefSeq Version	XM_024446434.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thymocyte selection associated (THEMIS), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024446434.1

ORF Insert Sequence:

ATGGAAGAAA TCACAAGGAC CATTCATATT GGACCAAGTA GACTAGGGCA TCCTTGCTTC 
TATCATCAGA AGGATATAAA ACTAGAGAAC CTCATCATAA AGCAGGGTGA GCAAATCATG 
CTCAACTCAG TTGAAGAGAT TGATGGAGAA ATAATGGTGA GCTGTGCAGT AGCAAGGAAT 
CATCAAACTC ACTCATTTAA TTTGCCTTTG TCACAAGAAG GAGAATTCTA CGAGTGTGAA 
GATGAACGTA TTTACACTCT AAAGGAGATT GTTGAATGGA AGATTCCTAA GAACAGAACA 
AGAACTGTAA ACCTTACAGA TTTTTCAAAT AAGTGGGACT CAACGAATCC ATTTCCTAAA 
GACTTTTATG GTACCCTGAT TCTCAAGCCT GTTTATGAAA TTCAAGGTGT GATGAAATTT 
CGAAAAGATA TAATCCGCAT CCTCCCCAGT CTAGATGTCG AAGTCAAAGA CATCACTGAT 
TCTTACGATG CTAACTGGTT TCTTCAGCTG TTATCAACAG AAGATCTTTT TGAAATGACT 
AGTAAAGAGT TCCCCATAGT GACTGAAGTC ATAGAAGCAC CTGAAGGAAA CCACCTGCCC 
CAAAGCATTT TACAGCCTGG GAAAACCATT GTGATCCACA AAAAGTACCA GGCATCAAGA 
ATCTTAGCTT CAGAAATTAG AAGCAATTTT CCTAAAAGAC ACTTCTTGAT CCCCACTAGC 
TATAAAGGCA AGTTCAAGCG GCGACCGAGG GAGTTCCCAA CGGCCTATGA CCTAGAGATC 
GCTAAGAGTG AAAAGGAGCC TCTTCACGTG GTGGCCACCA AAGCGTTTCA TTCCCCTCAT 
GACAAGCTGT CATCCGTATC TGTTGGGGAC CAGTTTCTGG TGCATCAGTC AGAGACGACT 
GAAGTCCTCT GTGAGGGAAT AAAAAAAGTG GTGAATGTTC TGGCCTGTGA AAAAATCCTC 
AAAAAGTCCT ATGAGGCTGC GCTGCTCCCT TTGTACATGG AAGGAGGTTT TGTAGAGGTG 
ATTCATGATA AGAAACAGTA CCCGATTTCT GAGCTCTGTA AACAGTTCCG TTTGCCCTTC 
AATGTGAAGG TGTCTGTCAG GGATCTTTCC ATTGAAGAGG ACGTGTTGGC TGCCACACCA 
GGACTGCAGT TGGAGGAGGA CATTACAGAC TCTTACCTAC TCATAAGTGA CTTTGCCAAC 
CCCACGGAGT GCTGGGAAAT TCCTGTGGGC CGCTTGAATA TGACTGTTCA GTTAGTTAGT 
AATTTCTCTA GGGATGCAGA ACCATTTCTA GTCAGGACTC TGGTAGAAGA GATCACTGAA 
GAGCAATATT ACATGATGCG GAGATATGAA AGCTCAGCCT CACATCCCCC ACCTCGCCCT 
CCGAAACACC CCTCAGTAGA GGAAACAAAG TTAACCCTGC TAACCTTAGC AGAAGAAAGG 
ACGGTAGACC TGCCCAAGTC TCCCAAGCGT CATCACGTAG ACATAACCAA GAAACTTCAC 
CCAAATCAAG CTGGCCTGGA TTCAAAAGTA CTGATTGGTA GTCAGAATGA TTTGGTGGAT 
GAAGAGAAAG AAAGGAGCAA CCGTGGGGCC ACAGCAATAG CAGAAACATT CAAAAATGAA 
AAACATCAAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THEMIS ( XM_011535816.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534118.1 Homo sapiens thymocyte selection associated (THEMIS), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu40804
Clone ID Related Accession (Same CDS sequence)	NM_001318531.1 , XM_024446434.1 , XM_011535816.1
Accession Version	XM_011535816.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein THEMIS isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA TCACAAGGAC CATTCATATT GGACCAAGTA GACTAGGGCA TCCTTGCTTC 
TATCATCAGA AGGATATAAA ACTAGAGAAC CTCATCATAA AGCAGGGTGA GCAAATCATG 
CTCAACTCAG TTGAAGAGAT TGATGGAGAA ATAATGGTGA GCTGTGCAGT AGCAAGGAAT 
CATCAAACTC ACTCATTTAA TTTGCCTTTG TCACAAGAAG GAGAATTCTA CGAGTGTGAA 
GATGAACGTA TTTACACTCT AAAGGAGATT GTTGAATGGA AGATTCCTAA GAACAGAACA 
AGAACTGTAA ACCTTACAGA TTTTTCAAAT AAGTGGGACT CAACGAATCC ATTTCCTAAA 
GACTTTTATG GTACCCTGAT TCTCAAGCCT GTTTATGAAA TTCAAGGTGT GATGAAATTT 
CGAAAAGATA TAATCCGCAT CCTCCCCAGT CTAGATGTCG AAGTCAAAGA CATCACTGAT 
TCTTACGATG CTAACTGGTT TCTTCAGCTG TTATCAACAG AAGATCTTTT TGAAATGACT 
AGTAAAGAGT TCCCCATAGT GACTGAAGTC ATAGAAGCAC CTGAAGGAAA CCACCTGCCC 
CAAAGCATTT TACAGCCTGG GAAAACCATT GTGATCCACA AAAAGTACCA GGCATCAAGA 
ATCTTAGCTT CAGAAATTAG AAGCAATTTT CCTAAAAGAC ACTTCTTGAT CCCCACTAGC 
TATAAAGGCA AGTTCAAGCG GCGACCGAGG GAGTTCCCAA CGGCCTATGA CCTAGAGATC 
GCTAAGAGTG AAAAGGAGCC TCTTCACGTG GTGGCCACCA AAGCGTTTCA TTCCCCTCAT 
GACAAGCTGT CATCCGTATC TGTTGGGGAC CAGTTTCTGG TGCATCAGTC AGAGACGACT 
GAAGTCCTCT GTGAGGGAAT AAAAAAAGTG GTGAATGTTC TGGCCTGTGA AAAAATCCTC 
AAAAAGTCCT ATGAGGCTGC GCTGCTCCCT TTGTACATGG AAGGAGGTTT TGTAGAGGTG 
ATTCATGATA AGAAACAGTA CCCGATTTCT GAGCTCTGTA AACAGTTCCG TTTGCCCTTC 
AATGTGAAGG TGTCTGTCAG GGATCTTTCC ATTGAAGAGG ACGTGTTGGC TGCCACACCA 
GGACTGCAGT TGGAGGAGGA CATTACAGAC TCTTACCTAC TCATAAGTGA CTTTGCCAAC 
CCCACGGAGT GCTGGGAAAT TCCTGTGGGC CGCTTGAATA TGACTGTTCA GTTAGTTAGT 
AATTTCTCTA GGGATGCAGA ACCATTTCTA GTCAGGACTC TGGTAGAAGA GATCACTGAA 
GAGCAATATT ACATGATGCG GAGATATGAA AGCTCAGCCT CACATCCCCC ACCTCGCCCT 
CCGAAACACC CCTCAGTAGA GGAAACAAAG TTAACCCTGC TAACCTTAGC AGAAGAAAGG 
ACGGTAGACC TGCCCAAGTC TCCCAAGCGT CATCACGTAG ACATAACCAA GAAACTTCAC 
CCAAATCAAG CTGGCCTGGA TTCAAAAGTA CTGATTGGTA GTCAGAATGA TTTGGTGGAT 
GAAGAGAAAG AAAGGAGCAA CCGTGGGGCC ACAGCAATAG CAGAAACATT CAAAAATGAA 
AAACATCAAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534118.1
CDS	631..2265
Translation	MEEITRTIHIGPSRLGHPCFYHQKDIKLENLIIKQGEQIMLNSVEEIDGEIMVSCAVARNHQTHSFNLPLSQEGEFYECEDERIYTLKEIVEWKIPKNRTRTVNLTDFSNKWDSTNPFPKDFYGTLILKPVYEIQGVMKFRKDIIRILPSLDVEVKDITDSYDANWFLQLLSTEDLFEMTSKEFPIVTEVIEAPEGNHLPQSILQPGKTIVIHKKYQASRILASEIRSNFPKRHFLIPTSYKGKFKRRPREFPTAYDLEIAKSEKEPLHVVATKAFHSPHDKLSSVSVGDQFLVHQSETTEVLCEGIKKVVNVLACEKILKKSYEAALLPLYMEGGFVEVIHDKKQYPISELCKQFRLPFNVKVSVRDLSIEEDVLAATPGLQLEEDITDSYLLISDFANPTECWEIPVGRLNMTVQLVSNFSRDAEPFLVRTLVEEITEEQYYMMRRYESSASHPPPRPPKHPSVEETKLTLLTLAEERTVDLPKSPKRHHVDITKKLHPNQAGLDSKVLIGSQNDLVDEEKERSNRGATAIAETFKNEKHQK

Target ORF information:

RefSeq Version	XM_011535816.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thymocyte selection associated (THEMIS), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535816.1

ORF Insert Sequence:

ATGGAAGAAA TCACAAGGAC CATTCATATT GGACCAAGTA GACTAGGGCA TCCTTGCTTC 
TATCATCAGA AGGATATAAA ACTAGAGAAC CTCATCATAA AGCAGGGTGA GCAAATCATG 
CTCAACTCAG TTGAAGAGAT TGATGGAGAA ATAATGGTGA GCTGTGCAGT AGCAAGGAAT 
CATCAAACTC ACTCATTTAA TTTGCCTTTG TCACAAGAAG GAGAATTCTA CGAGTGTGAA 
GATGAACGTA TTTACACTCT AAAGGAGATT GTTGAATGGA AGATTCCTAA GAACAGAACA 
AGAACTGTAA ACCTTACAGA TTTTTCAAAT AAGTGGGACT CAACGAATCC ATTTCCTAAA 
GACTTTTATG GTACCCTGAT TCTCAAGCCT GTTTATGAAA TTCAAGGTGT GATGAAATTT 
CGAAAAGATA TAATCCGCAT CCTCCCCAGT CTAGATGTCG AAGTCAAAGA CATCACTGAT 
TCTTACGATG CTAACTGGTT TCTTCAGCTG TTATCAACAG AAGATCTTTT TGAAATGACT 
AGTAAAGAGT TCCCCATAGT GACTGAAGTC ATAGAAGCAC CTGAAGGAAA CCACCTGCCC 
CAAAGCATTT TACAGCCTGG GAAAACCATT GTGATCCACA AAAAGTACCA GGCATCAAGA 
ATCTTAGCTT CAGAAATTAG AAGCAATTTT CCTAAAAGAC ACTTCTTGAT CCCCACTAGC 
TATAAAGGCA AGTTCAAGCG GCGACCGAGG GAGTTCCCAA CGGCCTATGA CCTAGAGATC 
GCTAAGAGTG AAAAGGAGCC TCTTCACGTG GTGGCCACCA AAGCGTTTCA TTCCCCTCAT 
GACAAGCTGT CATCCGTATC TGTTGGGGAC CAGTTTCTGG TGCATCAGTC AGAGACGACT 
GAAGTCCTCT GTGAGGGAAT AAAAAAAGTG GTGAATGTTC TGGCCTGTGA AAAAATCCTC 
AAAAAGTCCT ATGAGGCTGC GCTGCTCCCT TTGTACATGG AAGGAGGTTT TGTAGAGGTG 
ATTCATGATA AGAAACAGTA CCCGATTTCT GAGCTCTGTA AACAGTTCCG TTTGCCCTTC 
AATGTGAAGG TGTCTGTCAG GGATCTTTCC ATTGAAGAGG ACGTGTTGGC TGCCACACCA 
GGACTGCAGT TGGAGGAGGA CATTACAGAC TCTTACCTAC TCATAAGTGA CTTTGCCAAC 
CCCACGGAGT GCTGGGAAAT TCCTGTGGGC CGCTTGAATA TGACTGTTCA GTTAGTTAGT 
AATTTCTCTA GGGATGCAGA ACCATTTCTA GTCAGGACTC TGGTAGAAGA GATCACTGAA 
GAGCAATATT ACATGATGCG GAGATATGAA AGCTCAGCCT CACATCCCCC ACCTCGCCCT 
CCGAAACACC CCTCAGTAGA GGAAACAAAG TTAACCCTGC TAACCTTAGC AGAAGAAAGG 
ACGGTAGACC TGCCCAAGTC TCCCAAGCGT CATCACGTAG ACATAACCAA GAAACTTCAC 
CCAAATCAAG CTGGCCTGGA TTCAAAAGTA CTGATTGGTA GTCAGAATGA TTTGGTGGAT 
GAAGAGAAAG AAAGGAGCAA CCGTGGGGCC ACAGCAATAG CAGAAACATT CAAAAATGAA 
AAACATCAAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THEMIS ( XM_024446433.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024302201.1 Homo sapiens thymocyte selection associated (THEMIS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu79835
Clone ID Related Accession (Same CDS sequence)	XM_024446433.1 , XM_011535813.2
Accession Version	XM_024446433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein THEMIS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTAT CACTGGAAGA ATTCGTCCAC TCCCTTGACC TCAGGACCCT ACCCAGGGTT 
CTAGAAATCC AGGCAGGCAT CTATCTTGAA GGCTCTATTT ATGAAATGTT TGGAAATGAA 
TGCTGTTTTT CAACAGGAGA AGTGATTAAA ATTACTGGTC TCAAAGTTAA GAAGATCATA 
GCTGAAATTT GTGAGCAGAT TGAAGGTTGT GAGTCTCTAC AGCCATTTGA ACTGCCTATG 
AATTTTCCAG GGACTTTTGT TAAAAGAAGC AAGGAATGTC TTTTTAAGAT TGTGGCTGAT 
AAAACTCCAT ACCTTACTAT GGAAGAAATC ACAAGGACCA TTCATATTGG ACCAAGTAGA 
CTAGGGCATC CTTGCTTCTA TCATCAGAAG GATATAAAAC TAGAGAACCT CATCATAAAG 
CAGGGTGAGC AAATCATGCT CAACTCAGTT GAAGAGATTG ATGGAGAAAT AATGGTGAGC 
TGTGCAGTAG CAAGGAATCA TCAAACTCAC TCATTTAATT TGCCTTTGTC ACAAGAAGGA 
GAATTCTACG AGTGTGAAGA TGAACGTATT TACACTCTAA AGGAGATTGT TGAATGGAAG 
ATTCCTAAGA ACAGAACAAG AACTGTAAAC CTTACAGATT TTTCAAATAA GTGGGACTCA 
ACGAATCCAT TTCCTAAAGA CTTTTATGGT ACCCTGATTC TCAAGCCTGT TTATGAAATT 
CAAGGTGTGA TGAAATTTCG AAAAGATATA ATCCGCATCC TCCCCAGTCT AGATGTCGAA 
GTCAAAGACA TCACTGATTC TTACGATGCT AACTGGTTTC TTCAGCTGTT ATCAACAGAA 
GATCTTTTTG AAATGACTAG TAAAGAGTTC CCCATAGTGA CTGAAGTCAT AGAAGCACCT 
GAAGGAAACC ACCTGCCCCA AAGCATTTTA CAGCCTGGGA AAACCATTGT GATCCACAAA 
AAGTACCAGG CATCAAGAAT CTTAGCTTCA GAAATTAGAA GCAATTTTCC TAAAAGACAC 
TTCTTGATCC CCACTAGCTA TAAAGGCAAG TTCAAGCGGC GACCGAGGGA GTTCCCAACG 
GCCTATGACC TAGAGATCGC TAAGAGTGAA AAGGAGCCTC TTCACGTGGT GGCCACCAAA 
GCGTTTCATT CCCCTCATGA CAAGCTGTCA TCCGTATCTG TTGGGGACCA GTTTCTGGTG 
CATCAGTCAG AGACGACTGA AGTCCTCTGT GAGGGAATAA AAAAAGTGGT GAATGTTCTG 
GCCTGTGAAA AAATCCTCAA AAAGTCCTAT GAGGCTGCGC TGCTCCCTTT GTACATGGAA 
GGAGGTTTTG TAGAGGTGAT TCATGATAAG AAACAGTACC CGATTTCTGA GCTCTGTAAA 
CAGTTCCGTT TGCCCTTCAA TGTGAAGGTG TCTGTCAGGG ATCTTTCCAT TGAAGAGGAC 
GTGTTGGCTG CCACACCAGG ACTGCAGTTG GAGGAGGACA TTACAGACTC TTACCTACTC 
ATAAGTGACT TTGCCAACCC CACGGAGTGC TGGGAAATTC CTGTGGGCCG CTTGAATATG 
ACTGTTCAGT TAGTTAGTAA TTTCTCTAGG GATGCAGAAC CATTTCTAGT CAGGACTCTG 
GTAGAAGAGA TCACTGAAGA GCAATATTAC ATGATGCGGA GATATGAAAG CTCAGCCTCA 
CATCCCCCAC CTCGCCCTCC GAAACACCCC TCAGTAGAGG AAACAAAGTT AACCCTGCTA 
ACCTTAGCAG AAGAAAGGAC GGTAGACCTG CCCAAGTCTC CCAAGCGTCA TCACGTAGAC 
ATAACCAAGA AACTTCACCC AAATCAAGCT GGCCTGGATT CAAAAGTACT GATTGGTAGT 
CAGAATGATT TGGTGGATGA AGAGAAAGAA AGGAGCAACC GTGGGGCCAC AGCAATAGCA 
GAAACATTCA AAAATGAAAA ACATCAAAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024302201.1
CDS	317..2269
Translation	MALSLEEFVHSLDLRTLPRVLEIQAGIYLEGSIYEMFGNECCFSTGEVIKITGLKVKKIIAEICEQIEGCESLQPFELPMNFPGTFVKRSKECLFKIVADKTPYLTMEEITRTIHIGPSRLGHPCFYHQKDIKLENLIIKQGEQIMLNSVEEIDGEIMVSCAVARNHQTHSFNLPLSQEGEFYECEDERIYTLKEIVEWKIPKNRTRTVNLTDFSNKWDSTNPFPKDFYGTLILKPVYEIQGVMKFRKDIIRILPSLDVEVKDITDSYDANWFLQLLSTEDLFEMTSKEFPIVTEVIEAPEGNHLPQSILQPGKTIVIHKKYQASRILASEIRSNFPKRHFLIPTSYKGKFKRRPREFPTAYDLEIAKSEKEPLHVVATKAFHSPHDKLSSVSVGDQFLVHQSETTEVLCEGIKKVVNVLACEKILKKSYEAALLPLYMEGGFVEVIHDKKQYPISELCKQFRLPFNVKVSVRDLSIEEDVLAATPGLQLEEDITDSYLLISDFANPTECWEIPVGRLNMTVQLVSNFSRDAEPFLVRTLVEEITEEQYYMMRRYESSASHPPPRPPKHPSVEETKLTLLTLAEERTVDLPKSPKRHHVDITKKLHPNQAGLDSKVLIGSQNDLVDEEKERSNRGATAIAETFKNEKHQK

Target ORF information:

RefSeq Version	XM_024446433.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thymocyte selection associated (THEMIS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024446433.1

ORF Insert Sequence:

ATGGCATTAT CACTGGAAGA ATTCGTCCAC TCCCTTGACC TCAGGACCCT ACCCAGGGTT 
CTAGAAATCC AGGCAGGCAT CTATCTTGAA GGCTCTATTT ATGAAATGTT TGGAAATGAA 
TGCTGTTTTT CAACAGGAGA AGTGATTAAA ATTACTGGTC TCAAAGTTAA GAAGATCATA 
GCTGAAATTT GTGAGCAGAT TGAAGGTTGT GAGTCTCTAC AGCCATTTGA ACTGCCTATG 
AATTTTCCAG GGACTTTTGT TAAAAGAAGC AAGGAATGTC TTTTTAAGAT TGTGGCTGAT 
AAAACTCCAT ACCTTACTAT GGAAGAAATC ACAAGGACCA TTCATATTGG ACCAAGTAGA 
CTAGGGCATC CTTGCTTCTA TCATCAGAAG GATATAAAAC TAGAGAACCT CATCATAAAG 
CAGGGTGAGC AAATCATGCT CAACTCAGTT GAAGAGATTG ATGGAGAAAT AATGGTGAGC 
TGTGCAGTAG CAAGGAATCA TCAAACTCAC TCATTTAATT TGCCTTTGTC ACAAGAAGGA 
GAATTCTACG AGTGTGAAGA TGAACGTATT TACACTCTAA AGGAGATTGT TGAATGGAAG 
ATTCCTAAGA ACAGAACAAG AACTGTAAAC CTTACAGATT TTTCAAATAA GTGGGACTCA 
ACGAATCCAT TTCCTAAAGA CTTTTATGGT ACCCTGATTC TCAAGCCTGT TTATGAAATT 
CAAGGTGTGA TGAAATTTCG AAAAGATATA ATCCGCATCC TCCCCAGTCT AGATGTCGAA 
GTCAAAGACA TCACTGATTC TTACGATGCT AACTGGTTTC TTCAGCTGTT ATCAACAGAA 
GATCTTTTTG AAATGACTAG TAAAGAGTTC CCCATAGTGA CTGAAGTCAT AGAAGCACCT 
GAAGGAAACC ACCTGCCCCA AAGCATTTTA CAGCCTGGGA AAACCATTGT GATCCACAAA 
AAGTACCAGG CATCAAGAAT CTTAGCTTCA GAAATTAGAA GCAATTTTCC TAAAAGACAC 
TTCTTGATCC CCACTAGCTA TAAAGGCAAG TTCAAGCGGC GACCGAGGGA GTTCCCAACG 
GCCTATGACC TAGAGATCGC TAAGAGTGAA AAGGAGCCTC TTCACGTGGT GGCCACCAAA 
GCGTTTCATT CCCCTCATGA CAAGCTGTCA TCCGTATCTG TTGGGGACCA GTTTCTGGTG 
CATCAGTCAG AGACGACTGA AGTCCTCTGT GAGGGAATAA AAAAAGTGGT GAATGTTCTG 
GCCTGTGAAA AAATCCTCAA AAAGTCCTAT GAGGCTGCGC TGCTCCCTTT GTACATGGAA 
GGAGGTTTTG TAGAGGTGAT TCATGATAAG AAACAGTACC CGATTTCTGA GCTCTGTAAA 
CAGTTCCGTT TGCCCTTCAA TGTGAAGGTG TCTGTCAGGG ATCTTTCCAT TGAAGAGGAC 
GTGTTGGCTG CCACACCAGG ACTGCAGTTG GAGGAGGACA TTACAGACTC TTACCTACTC 
ATAAGTGACT TTGCCAACCC CACGGAGTGC TGGGAAATTC CTGTGGGCCG CTTGAATATG 
ACTGTTCAGT TAGTTAGTAA TTTCTCTAGG GATGCAGAAC CATTTCTAGT CAGGACTCTG 
GTAGAAGAGA TCACTGAAGA GCAATATTAC ATGATGCGGA GATATGAAAG CTCAGCCTCA 
CATCCCCCAC CTCGCCCTCC GAAACACCCC TCAGTAGAGG AAACAAAGTT AACCCTGCTA 
ACCTTAGCAG AAGAAAGGAC GGTAGACCTG CCCAAGTCTC CCAAGCGTCA TCACGTAGAC 
ATAACCAAGA AACTTCACCC AAATCAAGCT GGCCTGGATT CAAAAGTACT GATTGGTAGT 
CAGAATGATT TGGTGGATGA AGAGAAAGAA AGGAGCAACC GTGGGGCCAC AGCAATAGCA 
GAAACATTCA AAAATGAAAA ACATCAAAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THEMIS ( XM_011535813.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534115.1 Homo sapiens thymocyte selection associated (THEMIS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu79835
Clone ID Related Accession (Same CDS sequence)	XM_024446433.1 , XM_011535813.2
Accession Version	XM_011535813.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein THEMIS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535813.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTAT CACTGGAAGA ATTCGTCCAC TCCCTTGACC TCAGGACCCT ACCCAGGGTT 
CTAGAAATCC AGGCAGGCAT CTATCTTGAA GGCTCTATTT ATGAAATGTT TGGAAATGAA 
TGCTGTTTTT CAACAGGAGA AGTGATTAAA ATTACTGGTC TCAAAGTTAA GAAGATCATA 
GCTGAAATTT GTGAGCAGAT TGAAGGTTGT GAGTCTCTAC AGCCATTTGA ACTGCCTATG 
AATTTTCCAG GGACTTTTGT TAAAAGAAGC AAGGAATGTC TTTTTAAGAT TGTGGCTGAT 
AAAACTCCAT ACCTTACTAT GGAAGAAATC ACAAGGACCA TTCATATTGG ACCAAGTAGA 
CTAGGGCATC CTTGCTTCTA TCATCAGAAG GATATAAAAC TAGAGAACCT CATCATAAAG 
CAGGGTGAGC AAATCATGCT CAACTCAGTT GAAGAGATTG ATGGAGAAAT AATGGTGAGC 
TGTGCAGTAG CAAGGAATCA TCAAACTCAC TCATTTAATT TGCCTTTGTC ACAAGAAGGA 
GAATTCTACG AGTGTGAAGA TGAACGTATT TACACTCTAA AGGAGATTGT TGAATGGAAG 
ATTCCTAAGA ACAGAACAAG AACTGTAAAC CTTACAGATT TTTCAAATAA GTGGGACTCA 
ACGAATCCAT TTCCTAAAGA CTTTTATGGT ACCCTGATTC TCAAGCCTGT TTATGAAATT 
CAAGGTGTGA TGAAATTTCG AAAAGATATA ATCCGCATCC TCCCCAGTCT AGATGTCGAA 
GTCAAAGACA TCACTGATTC TTACGATGCT AACTGGTTTC TTCAGCTGTT ATCAACAGAA 
GATCTTTTTG AAATGACTAG TAAAGAGTTC CCCATAGTGA CTGAAGTCAT AGAAGCACCT 
GAAGGAAACC ACCTGCCCCA AAGCATTTTA CAGCCTGGGA AAACCATTGT GATCCACAAA 
AAGTACCAGG CATCAAGAAT CTTAGCTTCA GAAATTAGAA GCAATTTTCC TAAAAGACAC 
TTCTTGATCC CCACTAGCTA TAAAGGCAAG TTCAAGCGGC GACCGAGGGA GTTCCCAACG 
GCCTATGACC TAGAGATCGC TAAGAGTGAA AAGGAGCCTC TTCACGTGGT GGCCACCAAA 
GCGTTTCATT CCCCTCATGA CAAGCTGTCA TCCGTATCTG TTGGGGACCA GTTTCTGGTG 
CATCAGTCAG AGACGACTGA AGTCCTCTGT GAGGGAATAA AAAAAGTGGT GAATGTTCTG 
GCCTGTGAAA AAATCCTCAA AAAGTCCTAT GAGGCTGCGC TGCTCCCTTT GTACATGGAA 
GGAGGTTTTG TAGAGGTGAT TCATGATAAG AAACAGTACC CGATTTCTGA GCTCTGTAAA 
CAGTTCCGTT TGCCCTTCAA TGTGAAGGTG TCTGTCAGGG ATCTTTCCAT TGAAGAGGAC 
GTGTTGGCTG CCACACCAGG ACTGCAGTTG GAGGAGGACA TTACAGACTC TTACCTACTC 
ATAAGTGACT TTGCCAACCC CACGGAGTGC TGGGAAATTC CTGTGGGCCG CTTGAATATG 
ACTGTTCAGT TAGTTAGTAA TTTCTCTAGG GATGCAGAAC CATTTCTAGT CAGGACTCTG 
GTAGAAGAGA TCACTGAAGA GCAATATTAC ATGATGCGGA GATATGAAAG CTCAGCCTCA 
CATCCCCCAC CTCGCCCTCC GAAACACCCC TCAGTAGAGG AAACAAAGTT AACCCTGCTA 
ACCTTAGCAG AAGAAAGGAC GGTAGACCTG CCCAAGTCTC CCAAGCGTCA TCACGTAGAC 
ATAACCAAGA AACTTCACCC AAATCAAGCT GGCCTGGATT CAAAAGTACT GATTGGTAGT 
CAGAATGATT TGGTGGATGA AGAGAAAGAA AGGAGCAACC GTGGGGCCAC AGCAATAGCA 
GAAACATTCA AAAATGAAAA ACATCAAAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534115.1
CDS	317..2269
Translation	MALSLEEFVHSLDLRTLPRVLEIQAGIYLEGSIYEMFGNECCFSTGEVIKITGLKVKKIIAEICEQIEGCESLQPFELPMNFPGTFVKRSKECLFKIVADKTPYLTMEEITRTIHIGPSRLGHPCFYHQKDIKLENLIIKQGEQIMLNSVEEIDGEIMVSCAVARNHQTHSFNLPLSQEGEFYECEDERIYTLKEIVEWKIPKNRTRTVNLTDFSNKWDSTNPFPKDFYGTLILKPVYEIQGVMKFRKDIIRILPSLDVEVKDITDSYDANWFLQLLSTEDLFEMTSKEFPIVTEVIEAPEGNHLPQSILQPGKTIVIHKKYQASRILASEIRSNFPKRHFLIPTSYKGKFKRRPREFPTAYDLEIAKSEKEPLHVVATKAFHSPHDKLSSVSVGDQFLVHQSETTEVLCEGIKKVVNVLACEKILKKSYEAALLPLYMEGGFVEVIHDKKQYPISELCKQFRLPFNVKVSVRDLSIEEDVLAATPGLQLEEDITDSYLLISDFANPTECWEIPVGRLNMTVQLVSNFSRDAEPFLVRTLVEEITEEQYYMMRRYESSASHPPPRPPKHPSVEETKLTLLTLAEERTVDLPKSPKRHHVDITKKLHPNQAGLDSKVLIGSQNDLVDEEKERSNRGATAIAETFKNEKHQK

Target ORF information:

RefSeq Version	XM_011535813.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thymocyte selection associated (THEMIS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535813.2

ORF Insert Sequence:

ATGGCATTAT CACTGGAAGA ATTCGTCCAC TCCCTTGACC TCAGGACCCT ACCCAGGGTT 
CTAGAAATCC AGGCAGGCAT CTATCTTGAA GGCTCTATTT ATGAAATGTT TGGAAATGAA 
TGCTGTTTTT CAACAGGAGA AGTGATTAAA ATTACTGGTC TCAAAGTTAA GAAGATCATA 
GCTGAAATTT GTGAGCAGAT TGAAGGTTGT GAGTCTCTAC AGCCATTTGA ACTGCCTATG 
AATTTTCCAG GGACTTTTGT TAAAAGAAGC AAGGAATGTC TTTTTAAGAT TGTGGCTGAT 
AAAACTCCAT ACCTTACTAT GGAAGAAATC ACAAGGACCA TTCATATTGG ACCAAGTAGA 
CTAGGGCATC CTTGCTTCTA TCATCAGAAG GATATAAAAC TAGAGAACCT CATCATAAAG 
CAGGGTGAGC AAATCATGCT CAACTCAGTT GAAGAGATTG ATGGAGAAAT AATGGTGAGC 
TGTGCAGTAG CAAGGAATCA TCAAACTCAC TCATTTAATT TGCCTTTGTC ACAAGAAGGA 
GAATTCTACG AGTGTGAAGA TGAACGTATT TACACTCTAA AGGAGATTGT TGAATGGAAG 
ATTCCTAAGA ACAGAACAAG AACTGTAAAC CTTACAGATT TTTCAAATAA GTGGGACTCA 
ACGAATCCAT TTCCTAAAGA CTTTTATGGT ACCCTGATTC TCAAGCCTGT TTATGAAATT 
CAAGGTGTGA TGAAATTTCG AAAAGATATA ATCCGCATCC TCCCCAGTCT AGATGTCGAA 
GTCAAAGACA TCACTGATTC TTACGATGCT AACTGGTTTC TTCAGCTGTT ATCAACAGAA 
GATCTTTTTG AAATGACTAG TAAAGAGTTC CCCATAGTGA CTGAAGTCAT AGAAGCACCT 
GAAGGAAACC ACCTGCCCCA AAGCATTTTA CAGCCTGGGA AAACCATTGT GATCCACAAA 
AAGTACCAGG CATCAAGAAT CTTAGCTTCA GAAATTAGAA GCAATTTTCC TAAAAGACAC 
TTCTTGATCC CCACTAGCTA TAAAGGCAAG TTCAAGCGGC GACCGAGGGA GTTCCCAACG 
GCCTATGACC TAGAGATCGC TAAGAGTGAA AAGGAGCCTC TTCACGTGGT GGCCACCAAA 
GCGTTTCATT CCCCTCATGA CAAGCTGTCA TCCGTATCTG TTGGGGACCA GTTTCTGGTG 
CATCAGTCAG AGACGACTGA AGTCCTCTGT GAGGGAATAA AAAAAGTGGT GAATGTTCTG 
GCCTGTGAAA AAATCCTCAA AAAGTCCTAT GAGGCTGCGC TGCTCCCTTT GTACATGGAA 
GGAGGTTTTG TAGAGGTGAT TCATGATAAG AAACAGTACC CGATTTCTGA GCTCTGTAAA 
CAGTTCCGTT TGCCCTTCAA TGTGAAGGTG TCTGTCAGGG ATCTTTCCAT TGAAGAGGAC 
GTGTTGGCTG CCACACCAGG ACTGCAGTTG GAGGAGGACA TTACAGACTC TTACCTACTC 
ATAAGTGACT TTGCCAACCC CACGGAGTGC TGGGAAATTC CTGTGGGCCG CTTGAATATG 
ACTGTTCAGT TAGTTAGTAA TTTCTCTAGG GATGCAGAAC CATTTCTAGT CAGGACTCTG 
GTAGAAGAGA TCACTGAAGA GCAATATTAC ATGATGCGGA GATATGAAAG CTCAGCCTCA 
CATCCCCCAC CTCGCCCTCC GAAACACCCC TCAGTAGAGG AAACAAAGTT AACCCTGCTA 
ACCTTAGCAG AAGAAAGGAC GGTAGACCTG CCCAAGTCTC CCAAGCGTCA TCACGTAGAC 
ATAACCAAGA AACTTCACCC AAATCAAGCT GGCCTGGATT CAAAAGTACT GATTGGTAGT 
CAGAATGATT TGGTGGATGA AGAGAAAGAA AGGAGCAACC GTGGGGCCAC AGCAATAGCA 
GAAACATTCA AAAATGAAAA ACATCAAAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THEMIS ( XM_011535814.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534116.1 Homo sapiens thymocyte selection associated (THEMIS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu79836
Clone ID Related Accession (Same CDS sequence)	XM_011535814.2
Accession Version	XM_011535814.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein THEMIS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535814.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGAA ATGAATGCTG TTTTTCAACA GGAGAAGTGA TTAAAATTAC TGGTCTCAAA 
GTTAAGAAGA TCATAGCTGA AATTTGTGAG CAGATTGAAG GTTGTGAGTC TCTACAGCCA 
TTTGAACTGC CTATGAATTT TCCAGGGACT TTTGTTAAAA GAAGCAAGGA ATGTCTTTTT 
AAGATTGTGG CTGATAAAAC TCCATACCTT ACTATGGAAG AAATCACAAG GACCATTCAT 
ATTGGACCAA GTAGACTAGG GCATCCTTGC TTCTATCATC AGAAGGATAT AAAACTAGAG 
AACCTCATCA TAAAGCAGGG TGAGCAAATC ATGCTCAACT CAGTTGAAGA GATTGATGGA 
GAAATAATGG TGAGCTGTGC AGTAGCAAGG AATCATCAAA CTCACTCATT TAATTTGCCT 
TTGTCACAAG AAGGAGAATT CTACGAGTGT GAAGATGAAC GTATTTACAC TCTAAAGGAG 
ATTGTTGAAT GGAAGATTCC TAAGAACAGA ACAAGAACTG TAAACCTTAC AGATTTTTCA 
AATAAGTGGG ACTCAACGAA TCCATTTCCT AAAGACTTTT ATGGTACCCT GATTCTCAAG 
CCTGTTTATG AAATTCAAGG TGTGATGAAA TTTCGAAAAG ATATAATCCG CATCCTCCCC 
AGTCTAGATG TCGAAGTCAA AGACATCACT GATTCTTACG ATGCTAACTG GTTTCTTCAG 
CTGTTATCAA CAGAAGATCT TTTTGAAATG ACTAGTAAAG AGTTCCCCAT AGTGACTGAA 
GTCATAGAAG CACCTGAAGG AAACCACCTG CCCCAAAGCA TTTTACAGCC TGGGAAAACC 
ATTGTGATCC ACAAAAAGTA CCAGGCATCA AGAATCTTAG CTTCAGAAAT TAGAAGCAAT 
TTTCCTAAAA GACACTTCTT GATCCCCACT AGCTATAAAG GCAAGTTCAA GCGGCGACCG 
AGGGAGTTCC CAACGGCCTA TGACCTAGAG ATCGCTAAGA GTGAAAAGGA GCCTCTTCAC 
GTGGTGGCCA CCAAAGCGTT TCATTCCCCT CATGACAAGC TGTCATCCGT ATCTGTTGGG 
GACCAGTTTC TGGTGCATCA GTCAGAGACG ACTGAAGTCC TCTGTGAGGG AATAAAAAAA 
GTGGTGAATG TTCTGGCCTG TGAAAAAATC CTCAAAAAGT CCTATGAGGC TGCGCTGCTC 
CCTTTGTACA TGGAAGGAGG TTTTGTAGAG GTGATTCATG ATAAGAAACA GTACCCGATT 
TCTGAGCTCT GTAAACAGTT CCGTTTGCCC TTCAATGTGA AGGTGTCTGT CAGGGATCTT 
TCCATTGAAG AGGACGTGTT GGCTGCCACA CCAGGACTGC AGTTGGAGGA GGACATTACA 
GACTCTTACC TACTCATAAG TGACTTTGCC AACCCCACGG AGTGCTGGGA AATTCCTGTG 
GGCCGCTTGA ATATGACTGT TCAGTTAGTT AGTAATTTCT CTAGGGATGC AGAACCATTT 
CTAGTCAGGA CTCTGGTAGA AGAGATCACT GAAGAGCAAT ATTACATGAT GCGGAGATAT 
GAAAGCTCAG CCTCACATCC CCCACCTCGC CCTCCGAAAC ACCCCTCAGT AGAGGAAACA 
AAGTTAACCC TGCTAACCTT AGCAGAAGAA AGGACGGTAG ACCTGCCCAA GTCTCCCAAG 
CGTCATCACG TAGACATAAC CAAGAAACTT CACCCAAATC AAGCTGGCCT GGATTCAAAA 
GTACTGATTG GTAGTCAGAA TGATTTGGTG GATGAAGAGA AAGAAAGGAG CAACCGTGGG 
GCCACAGCAA TAGCAGAAAC ATTCAAAAAT GAAAAACATC AAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534116.1
CDS	193..2040
Translation	MFGNECCFSTGEVIKITGLKVKKIIAEICEQIEGCESLQPFELPMNFPGTFVKRSKECLFKIVADKTPYLTMEEITRTIHIGPSRLGHPCFYHQKDIKLENLIIKQGEQIMLNSVEEIDGEIMVSCAVARNHQTHSFNLPLSQEGEFYECEDERIYTLKEIVEWKIPKNRTRTVNLTDFSNKWDSTNPFPKDFYGTLILKPVYEIQGVMKFRKDIIRILPSLDVEVKDITDSYDANWFLQLLSTEDLFEMTSKEFPIVTEVIEAPEGNHLPQSILQPGKTIVIHKKYQASRILASEIRSNFPKRHFLIPTSYKGKFKRRPREFPTAYDLEIAKSEKEPLHVVATKAFHSPHDKLSSVSVGDQFLVHQSETTEVLCEGIKKVVNVLACEKILKKSYEAALLPLYMEGGFVEVIHDKKQYPISELCKQFRLPFNVKVSVRDLSIEEDVLAATPGLQLEEDITDSYLLISDFANPTECWEIPVGRLNMTVQLVSNFSRDAEPFLVRTLVEEITEEQYYMMRRYESSASHPPPRPPKHPSVEETKLTLLTLAEERTVDLPKSPKRHHVDITKKLHPNQAGLDSKVLIGSQNDLVDEEKERSNRGATAIAETFKNEKHQK

Target ORF information:

RefSeq Version	XM_011535814.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thymocyte selection associated (THEMIS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535814.2

ORF Insert Sequence:

ATGTTTGGAA ATGAATGCTG TTTTTCAACA GGAGAAGTGA TTAAAATTAC TGGTCTCAAA 
GTTAAGAAGA TCATAGCTGA AATTTGTGAG CAGATTGAAG GTTGTGAGTC TCTACAGCCA 
TTTGAACTGC CTATGAATTT TCCAGGGACT TTTGTTAAAA GAAGCAAGGA ATGTCTTTTT 
AAGATTGTGG CTGATAAAAC TCCATACCTT ACTATGGAAG AAATCACAAG GACCATTCAT 
ATTGGACCAA GTAGACTAGG GCATCCTTGC TTCTATCATC AGAAGGATAT AAAACTAGAG 
AACCTCATCA TAAAGCAGGG TGAGCAAATC ATGCTCAACT CAGTTGAAGA GATTGATGGA 
GAAATAATGG TGAGCTGTGC AGTAGCAAGG AATCATCAAA CTCACTCATT TAATTTGCCT 
TTGTCACAAG AAGGAGAATT CTACGAGTGT GAAGATGAAC GTATTTACAC TCTAAAGGAG 
ATTGTTGAAT GGAAGATTCC TAAGAACAGA ACAAGAACTG TAAACCTTAC AGATTTTTCA 
AATAAGTGGG ACTCAACGAA TCCATTTCCT AAAGACTTTT ATGGTACCCT GATTCTCAAG 
CCTGTTTATG AAATTCAAGG TGTGATGAAA TTTCGAAAAG ATATAATCCG CATCCTCCCC 
AGTCTAGATG TCGAAGTCAA AGACATCACT GATTCTTACG ATGCTAACTG GTTTCTTCAG 
CTGTTATCAA CAGAAGATCT TTTTGAAATG ACTAGTAAAG AGTTCCCCAT AGTGACTGAA 
GTCATAGAAG CACCTGAAGG AAACCACCTG CCCCAAAGCA TTTTACAGCC TGGGAAAACC 
ATTGTGATCC ACAAAAAGTA CCAGGCATCA AGAATCTTAG CTTCAGAAAT TAGAAGCAAT 
TTTCCTAAAA GACACTTCTT GATCCCCACT AGCTATAAAG GCAAGTTCAA GCGGCGACCG 
AGGGAGTTCC CAACGGCCTA TGACCTAGAG ATCGCTAAGA GTGAAAAGGA GCCTCTTCAC 
GTGGTGGCCA CCAAAGCGTT TCATTCCCCT CATGACAAGC TGTCATCCGT ATCTGTTGGG 
GACCAGTTTC TGGTGCATCA GTCAGAGACG ACTGAAGTCC TCTGTGAGGG AATAAAAAAA 
GTGGTGAATG TTCTGGCCTG TGAAAAAATC CTCAAAAAGT CCTATGAGGC TGCGCTGCTC 
CCTTTGTACA TGGAAGGAGG TTTTGTAGAG GTGATTCATG ATAAGAAACA GTACCCGATT 
TCTGAGCTCT GTAAACAGTT CCGTTTGCCC TTCAATGTGA AGGTGTCTGT CAGGGATCTT 
TCCATTGAAG AGGACGTGTT GGCTGCCACA CCAGGACTGC AGTTGGAGGA GGACATTACA 
GACTCTTACC TACTCATAAG TGACTTTGCC AACCCCACGG AGTGCTGGGA AATTCCTGTG 
GGCCGCTTGA ATATGACTGT TCAGTTAGTT AGTAATTTCT CTAGGGATGC AGAACCATTT 
CTAGTCAGGA CTCTGGTAGA AGAGATCACT GAAGAGCAAT ATTACATGAT GCGGAGATAT 
GAAAGCTCAG CCTCACATCC CCCACCTCGC CCTCCGAAAC ACCCCTCAGT AGAGGAAACA 
AAGTTAACCC TGCTAACCTT AGCAGAAGAA AGGACGGTAG ACCTGCCCAA GTCTCCCAAG 
CGTCATCACG TAGACATAAC CAAGAAACTT CACCCAAATC AAGCTGGCCT GGATTCAAAA 
GTACTGATTG GTAGTCAGAA TGATTTGGTG GATGAAGAGA AAGAAAGGAG CAACCGTGGG 
GCCACAGCAA TAGCAGAAAC ATTCAAAAAT GAAAAACATC AAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THEMIS ( XM_017010849.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866338.1 Homo sapiens thymocyte selection associated (THEMIS), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu96593
Clone ID Related Accession (Same CDS sequence)	XM_017010849.1
Accession Version	XM_017010849.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein THEMIS isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTAT CACTGGAAGA ATTCGTCCAC TCCCTTGACC TCAGGACCCT ACCCAGGGTT 
CTAGAAATCC AGGCAGGCAT CTATCTTGAA GGCTCTATTT ATGAAATGTT TGGAAATGAA 
TGCTGTTTTT CAACAGGAGA AGTGATTAAA ATTACTGGTC TCAAAGTTAA GAAGATCATA 
GCTGAAATTT GTGAGCAGAT TGAAGGTTGT GAGTCTCTAC AGCCATTTGA ACTGCCTATG 
AATTTTCCAG TTCGAAAAGA TATAATCCGC ATCCTCCCCA GTCTAGATGT CGAAGTCAAA 
GACATCACTG ATTCTTACGA TGCTAACTGG TTTCTTCAGC TGTTATCAAC AGAAGATCTT 
TTTGAAATGA CTAGTAAAGA GTTCCCCATA GTGACTGAAG TCATAGAAGC ACCTGAAGGA 
AACCACCTGC CCCAAAGCAT TTTACAGCCT GGGAAAACCA TTGTGATCCA CAAAAAGTAC 
CAGGCATCAA GAATCTTAGC TTCAGAAATT AGAAGCAATT TTCCTAAAAG ACACTTCTTG 
ATCCCCACTA GCTATAAAGG CAAGTTCAAG CGGCGACCGA GGGAGTTCCC AACGGCCTAT 
GACCTAGAGA TCGCTAAGAG TGAAAAGGAG CCTCTTCACG TGGTGGCCAC CAAAGCGTTT 
CATTCCCCTC ATGACAAGCT GTCATCCGTA TCTGTTGGGG ACCAGTTTCT GGTGCATCAG 
TCAGAGACGA CTGAAGTCCT CTGTGAGGGA ATAAAAAAAG TGGTGAATGT TCTGGCCTGT 
GAAAAAATCC TCAAAAAGTC CTATGAGGCT GCGCTGCTCC CTTTGTACAT GGAAGGAGGT 
TTTGTAGAGG TGATTCATGA TAAGAAACAG TACCCGATTT CTGAGCTCTG TAAACAGTTC 
CGTTTGCCCT TCAATGTGAA GGTGTCTGTC AGGGATCTTT CCATTGAAGA GGACGTGTTG 
GCTGCCACAC CAGGACTGCA GTTGGAGGAG GACATTACAG ACTCTTACCT ACTCATAAGT 
GACTTTGCCA ACCCCACGGA GTGCTGGGAA ATTCCTGTGG GCCGCTTGAA TATGACTGTT 
CAGTTAGTTA GTAATTTCTC TAGGGATGCA GAACCATTTC TAGTCAGGAC TCTGGTAGAA 
GAGATCACTG AAGAGCAATA TTACATGATG CGGAGATATG AAAGCTCAGC CTCACATCCC 
CCACCTCGCC CTCCGAAACA CCCCTCAGTA GAGGAAACAA AGTTAACCCT GCTAACCTTA 
GCAGAAGAAA GGACGGTAGA CCTGCCCAAG TCTCCCAAGC GTCATCACGT AGACATAACC 
AAGAAACTTC ACCCAAATCA AGCTGGCCTG GATTCAAAAG TACTGATTGG TAGTCAGAAT 
GATTTGGTGG ATGAAGAGAA AGAAAGGAGC AACCGTGGGG CCACAGCAAT AGCAGAAACA 
TTCAAAAATG AAAAACATCA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866338.1
CDS	317..1783
Translation	MALSLEEFVHSLDLRTLPRVLEIQAGIYLEGSIYEMFGNECCFSTGEVIKITGLKVKKIIAEICEQIEGCESLQPFELPMNFPVRKDIIRILPSLDVEVKDITDSYDANWFLQLLSTEDLFEMTSKEFPIVTEVIEAPEGNHLPQSILQPGKTIVIHKKYQASRILASEIRSNFPKRHFLIPTSYKGKFKRRPREFPTAYDLEIAKSEKEPLHVVATKAFHSPHDKLSSVSVGDQFLVHQSETTEVLCEGIKKVVNVLACEKILKKSYEAALLPLYMEGGFVEVIHDKKQYPISELCKQFRLPFNVKVSVRDLSIEEDVLAATPGLQLEEDITDSYLLISDFANPTECWEIPVGRLNMTVQLVSNFSRDAEPFLVRTLVEEITEEQYYMMRRYESSASHPPPRPPKHPSVEETKLTLLTLAEERTVDLPKSPKRHHVDITKKLHPNQAGLDSKVLIGSQNDLVDEEKERSNRGATAIAETFKNEKHQK

Target ORF information:

RefSeq Version	XM_017010849.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thymocyte selection associated (THEMIS), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010849.1

ORF Insert Sequence:

ATGGCATTAT CACTGGAAGA ATTCGTCCAC TCCCTTGACC TCAGGACCCT ACCCAGGGTT 
CTAGAAATCC AGGCAGGCAT CTATCTTGAA GGCTCTATTT ATGAAATGTT TGGAAATGAA 
TGCTGTTTTT CAACAGGAGA AGTGATTAAA ATTACTGGTC TCAAAGTTAA GAAGATCATA 
GCTGAAATTT GTGAGCAGAT TGAAGGTTGT GAGTCTCTAC AGCCATTTGA ACTGCCTATG 
AATTTTCCAG TTCGAAAAGA TATAATCCGC ATCCTCCCCA GTCTAGATGT CGAAGTCAAA 
GACATCACTG ATTCTTACGA TGCTAACTGG TTTCTTCAGC TGTTATCAAC AGAAGATCTT 
TTTGAAATGA CTAGTAAAGA GTTCCCCATA GTGACTGAAG TCATAGAAGC ACCTGAAGGA 
AACCACCTGC CCCAAAGCAT TTTACAGCCT GGGAAAACCA TTGTGATCCA CAAAAAGTAC 
CAGGCATCAA GAATCTTAGC TTCAGAAATT AGAAGCAATT TTCCTAAAAG ACACTTCTTG 
ATCCCCACTA GCTATAAAGG CAAGTTCAAG CGGCGACCGA GGGAGTTCCC AACGGCCTAT 
GACCTAGAGA TCGCTAAGAG TGAAAAGGAG CCTCTTCACG TGGTGGCCAC CAAAGCGTTT 
CATTCCCCTC ATGACAAGCT GTCATCCGTA TCTGTTGGGG ACCAGTTTCT GGTGCATCAG 
TCAGAGACGA CTGAAGTCCT CTGTGAGGGA ATAAAAAAAG TGGTGAATGT TCTGGCCTGT 
GAAAAAATCC TCAAAAAGTC CTATGAGGCT GCGCTGCTCC CTTTGTACAT GGAAGGAGGT 
TTTGTAGAGG TGATTCATGA TAAGAAACAG TACCCGATTT CTGAGCTCTG TAAACAGTTC 
CGTTTGCCCT TCAATGTGAA GGTGTCTGTC AGGGATCTTT CCATTGAAGA GGACGTGTTG 
GCTGCCACAC CAGGACTGCA GTTGGAGGAG GACATTACAG ACTCTTACCT ACTCATAAGT 
GACTTTGCCA ACCCCACGGA GTGCTGGGAA ATTCCTGTGG GCCGCTTGAA TATGACTGTT 
CAGTTAGTTA GTAATTTCTC TAGGGATGCA GAACCATTTC TAGTCAGGAC TCTGGTAGAA 
GAGATCACTG AAGAGCAATA TTACATGATG CGGAGATATG AAAGCTCAGC CTCACATCCC 
CCACCTCGCC CTCCGAAACA CCCCTCAGTA GAGGAAACAA AGTTAACCCT GCTAACCTTA 
GCAGAAGAAA GGACGGTAGA CCTGCCCAAG TCTCCCAAGC GTCATCACGT AGACATAACC 
AAGAAACTTC ACCCAAATCA AGCTGGCCTG GATTCAAAAG TACTGATTGG TAGTCAGAAT 
GATTTGGTGG ATGAAGAGAA AGAAAGGAGC AACCGTGGGG CCACAGCAAT AGCAGAAACA 
TTCAAAAATG AAAAACATCA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THEMIS ( XM_017010848.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866337.1 Homo sapiens thymocyte selection associated (THEMIS), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu96592
Clone ID Related Accession (Same CDS sequence)	XM_017010848.1
Accession Version	XM_017010848.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein THEMIS isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTAT CACTGGAAGA ATTCGTCCAC TCCCTTGACC TCAGGACCCT ACCCAGGGTT 
CTAGAAATCC AGGCAGGCAT CTATCTTGAA GGCTCTATTT ATGAAATGTT TGGAAATGAA 
TGCTGTTTTT CAACAGGAGA AGTGATTAAA ATTACTGGTC TCAAAGTTAA GAAGATCATA 
GCTGAAATTT GTGAGCAGAT TGAAGGTTGT GAGTCTCTAC AGCCATTTGA ACTGCCTATG 
AATTTTCCAG GGACTTTTGT TAAAAGAAGC AAGGAATGTC TTTTTAAGAT TGTGGCTGAT 
AAAACTCCAT ACCTTACTAT GGAAGAAATC ACAAGGACCA TTCATATTGG ACCAAGTAGA 
CTAGGGCATC CTTGCTTCTA TCATCAGAAG GATATAAAAC TAGAGAACCT CATCATAAAG 
CAGGGTGAGC AAATCATGCT CAACTCAGTT GAAGAGATTG ATGGAGAAAT AATGGTGAGC 
TGTGCAGTAG CAAGGAATCA TCAAACTCAC TCATTTAATT TGCCTTTGTC ACAAGAAGGA 
GAATTCTACG AGTGTGAAGA TGAACGTATT TACACTCTAA AGGAGATTGT TGAATGGAAG 
ATTCCTAAGA ACAGAACAAG AACTGTAAAC CTTACAGATT TTTCAAATAA GTGGGACTCA 
ACGAATCCAT TTCCTAAAGA CTTTTATGGT ACCCTGATTC TCAAGCCTGT TTATGAAATT 
CAAGGTGTGA TGAAATTTCG AAAAGATATA ATCCGCATCC TCCCCAGTCT AGATGTCGAA 
GTCAAAGACA TCACTGATTC TTACGATGCT AACTGGTTTC TTCAGCTGTT ATCAACAGAA 
GATCTTTTTG AAATGACTAG TAAAGAGTTC CCCATAGTGA CTGAAGTCAT AGAAGCACCT 
GAAGGAAACC ACCTGCCCCA AAGCATTTTA CAGCCTGGGA AAACCATTGT GATCCACAAA 
AAGTACCAGG CATCAAGAAT CTTAGCTTCA GAAATTAGAA GCAATTTTCC TAAAAGACAC 
TTCTTGATCC CCACTAGCTA TAAAGGCAAG TTCAAGCGGC GACCGAGGGA GTTCCCAACG 
GCCTATGACC TAGAGATCGC TAAGAGTGAA AAGGAGCCTC TTCACGTGGT GGCCACCAAA 
GCGTTTCATT CCCCTCATGA CAAGCTGTCA TCCGTATCTG TTGGGGACCA GTTTCTGGTG 
CATCAGTCAG AGACGACTGA AGTCCTCTGT GAGGGAATAA AAAAAGTGGT GAATGTTCTG 
GCCTGTGAAA AAATCCTCAA AAAGTCCTAT GAGGCTGCGC TGCTCCCTTT GTACATGGAA 
GGAGGTTTTG TAGAGGTGAT TCATGATAAG AAACAGTACC CGATTTCTGA GCTCTGTAAA 
CAGTTCCGTT TGCCCTTCAA TGTGAAGGTG TCTGTCAGGG ATCTTTCCAT TGAAGAGGAC 
GTGTTGGCTG CCACACCAGG ACTGCAGTTG GAGGAGGACA TTACAGACTC TTACCTACTC 
ATAAGTGACT TTGCCAACCC CACGGAGTGC TGGGAAATTC CTGTGGGCCG CTTGAATATG 
ACTGTTCAGT TAGTTAGTAA TTTCTCTAGG GATGCAGAAC CATTTCTAGT CAGGACTCTG 
GTAGAAGAGA TCACTGAAGA GCAATATTAC ATGATGCGGA GATATGAAAG CTCAGCCTCA 
CATCCCCCAC CTCGCCCTCC GAAACACCCC TCAGTAGAGG AAACAAAGTT AACCCTGCTA 
ACCTTAGCAG AAGAAAGGAC GGTAGACCTG CCCAAGTCTC CCAAGCATGA AGCCATCACC 
AGAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866337.1
CDS	317..2125
Translation	MALSLEEFVHSLDLRTLPRVLEIQAGIYLEGSIYEMFGNECCFSTGEVIKITGLKVKKIIAEICEQIEGCESLQPFELPMNFPGTFVKRSKECLFKIVADKTPYLTMEEITRTIHIGPSRLGHPCFYHQKDIKLENLIIKQGEQIMLNSVEEIDGEIMVSCAVARNHQTHSFNLPLSQEGEFYECEDERIYTLKEIVEWKIPKNRTRTVNLTDFSNKWDSTNPFPKDFYGTLILKPVYEIQGVMKFRKDIIRILPSLDVEVKDITDSYDANWFLQLLSTEDLFEMTSKEFPIVTEVIEAPEGNHLPQSILQPGKTIVIHKKYQASRILASEIRSNFPKRHFLIPTSYKGKFKRRPREFPTAYDLEIAKSEKEPLHVVATKAFHSPHDKLSSVSVGDQFLVHQSETTEVLCEGIKKVVNVLACEKILKKSYEAALLPLYMEGGFVEVIHDKKQYPISELCKQFRLPFNVKVSVRDLSIEEDVLAATPGLQLEEDITDSYLLISDFANPTECWEIPVGRLNMTVQLVSNFSRDAEPFLVRTLVEEITEEQYYMMRRYESSASHPPPRPPKHPSVEETKLTLLTLAEERTVDLPKSPKHEAITRS

Target ORF information:

RefSeq Version	XM_017010848.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thymocyte selection associated (THEMIS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010848.1

ORF Insert Sequence:

ATGGCATTAT CACTGGAAGA ATTCGTCCAC TCCCTTGACC TCAGGACCCT ACCCAGGGTT 
CTAGAAATCC AGGCAGGCAT CTATCTTGAA GGCTCTATTT ATGAAATGTT TGGAAATGAA 
TGCTGTTTTT CAACAGGAGA AGTGATTAAA ATTACTGGTC TCAAAGTTAA GAAGATCATA 
GCTGAAATTT GTGAGCAGAT TGAAGGTTGT GAGTCTCTAC AGCCATTTGA ACTGCCTATG 
AATTTTCCAG GGACTTTTGT TAAAAGAAGC AAGGAATGTC TTTTTAAGAT TGTGGCTGAT 
AAAACTCCAT ACCTTACTAT GGAAGAAATC ACAAGGACCA TTCATATTGG ACCAAGTAGA 
CTAGGGCATC CTTGCTTCTA TCATCAGAAG GATATAAAAC TAGAGAACCT CATCATAAAG 
CAGGGTGAGC AAATCATGCT CAACTCAGTT GAAGAGATTG ATGGAGAAAT AATGGTGAGC 
TGTGCAGTAG CAAGGAATCA TCAAACTCAC TCATTTAATT TGCCTTTGTC ACAAGAAGGA 
GAATTCTACG AGTGTGAAGA TGAACGTATT TACACTCTAA AGGAGATTGT TGAATGGAAG 
ATTCCTAAGA ACAGAACAAG AACTGTAAAC CTTACAGATT TTTCAAATAA GTGGGACTCA 
ACGAATCCAT TTCCTAAAGA CTTTTATGGT ACCCTGATTC TCAAGCCTGT TTATGAAATT 
CAAGGTGTGA TGAAATTTCG AAAAGATATA ATCCGCATCC TCCCCAGTCT AGATGTCGAA 
GTCAAAGACA TCACTGATTC TTACGATGCT AACTGGTTTC TTCAGCTGTT ATCAACAGAA 
GATCTTTTTG AAATGACTAG TAAAGAGTTC CCCATAGTGA CTGAAGTCAT AGAAGCACCT 
GAAGGAAACC ACCTGCCCCA AAGCATTTTA CAGCCTGGGA AAACCATTGT GATCCACAAA 
AAGTACCAGG CATCAAGAAT CTTAGCTTCA GAAATTAGAA GCAATTTTCC TAAAAGACAC 
TTCTTGATCC CCACTAGCTA TAAAGGCAAG TTCAAGCGGC GACCGAGGGA GTTCCCAACG 
GCCTATGACC TAGAGATCGC TAAGAGTGAA AAGGAGCCTC TTCACGTGGT GGCCACCAAA 
GCGTTTCATT CCCCTCATGA CAAGCTGTCA TCCGTATCTG TTGGGGACCA GTTTCTGGTG 
CATCAGTCAG AGACGACTGA AGTCCTCTGT GAGGGAATAA AAAAAGTGGT GAATGTTCTG 
GCCTGTGAAA AAATCCTCAA AAAGTCCTAT GAGGCTGCGC TGCTCCCTTT GTACATGGAA 
GGAGGTTTTG TAGAGGTGAT TCATGATAAG AAACAGTACC CGATTTCTGA GCTCTGTAAA 
CAGTTCCGTT TGCCCTTCAA TGTGAAGGTG TCTGTCAGGG ATCTTTCCAT TGAAGAGGAC 
GTGTTGGCTG CCACACCAGG ACTGCAGTTG GAGGAGGACA TTACAGACTC TTACCTACTC 
ATAAGTGACT TTGCCAACCC CACGGAGTGC TGGGAAATTC CTGTGGGCCG CTTGAATATG 
ACTGTTCAGT TAGTTAGTAA TTTCTCTAGG GATGCAGAAC CATTTCTAGT CAGGACTCTG 
GTAGAAGAGA TCACTGAAGA GCAATATTAC ATGATGCGGA GATATGAAAG CTCAGCCTCA 
CATCCCCCAC CTCGCCCTCC GAAACACCCC TCAGTAGAGG AAACAAAGTT AACCCTGCTA 
ACCTTAGCAG AAGAAAGGAC GGTAGACCTG CCCAAGTCTC CCAAGCATGA AGCCATCACC 
AGAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	THEMIS
Entrez Gene ID	387357
Full Name	thymocyte selection associated
Synonyms	C6orf190,C6orf207,GASP,SPOT,TSEPA
General protein information	Preferred Names thymocyte selection associated Names protein THEMIS GRB2-associated protein signaling phosphoprotein specific for T cells thymocyte selection pathway associated thymocyte-expressed molecule involved in selection
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q22.33
Summary	This gene encodes a protein that plays a regulatory role in both positive and negative T-cell selection during late thymocyte development. The protein functions through T-cell antigen receptor signaling, and is necessary for proper lineage commitment and maturation of T-cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009].
Disorder MIM:	613607

Homo sapiens (human)	THEMIS	NP_001158157.1
Danio rerio (zebrafish)	themis	XP_683025.3
Pan troglodytes (chimpanzee)	THEMIS	XP_003311524.1
Canis lupus familiaris (dog)	THEMIS	XP_541237.4
Bos taurus (cattle)	THEMIS	NP_001091585.1
Gallus gallus (chicken)	THEMIS	XP_001232459.3
Xenopus tropicalis (tropical clawed frog)	themis	XP_002940439.2
Rattus norvegicus (Norway rat)	Themis	XP_006227737.1
Mus musculus (house mouse)	Themis	NP_848781.1

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THEMIS cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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