Kntc1 cDNA ORF clone, Mus musculus(house mouse)

The following Kntc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kntc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu35846 XM_006530251.4
Latest version!
Mus musculus kinetochore associated 1 (Kntc1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu35847 XM_006530252.4
Latest version!
Mus musculus kinetochore associated 1 (Kntc1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu35848 XM_006530253.4
Latest version!
Mus musculus kinetochore associated 1 (Kntc1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu35849 XM_006530254.2
Latest version!
Mus musculus kinetochore associated 1 (Kntc1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu35844 XM_030254330.1
Latest version!
Mus musculus kinetochore associated 1 (Kntc1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu35845 XM_006530250.4
Latest version!
Mus musculus kinetochore associated 1 (Kntc1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu00882 NM_001042421.1
Latest version!
Mus musculus kinetochore associated 1 (Kntc1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu35844 XM_006530249.1
Latest version!
Mus musculus kinetochore associated 1 (Kntc1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
View Old Accession Versions >>
OMu35846 XM_006530251.3 Mus musculus kinetochore associated 1 (Kntc1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu35847 XM_006530252.3 Mus musculus kinetochore associated 1 (Kntc1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu35848 XM_006530253.3 Mus musculus kinetochore associated 1 (Kntc1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu35845 XM_006530250.3 Mus musculus kinetochore associated 1 (Kntc1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu35846
    Clone ID Related Accession (Same CDS sequence) XM_006530251.4 , XM_006530251.3
    Accession Version XM_006530251.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product kinetochore-associated protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530251.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTACAAGG CGGTGGTTCC CTGGAGTGCA GCCGTGGAGC AGCTGGTGAA ACAGCACCTG 
    GAAATGGACC ACCCCAAAGT CAAGTTATTG CAGGAAAGCT ACAAACTGAT GGAGATGAAG
    AAACTTTTGC GAGGGTATGG GATAAGAGAG GTGAATCTCC TGAACAAGGA AATAATGAGA
    GTGATCCGAT ACATTCTAAA GCAGGACATC CCGTCTTCTT TAGAAGATGC TCTGAAGGTG
    GCCCAGGGGT ACAGGCTGTC TGATGACGAA ATCTACAGCC TGAGAATCAT CGATCTGATT
    GACAGGGAGC AGGGGGGAGA TTGCCTCCTG CTACTGAAGT CTCTGCCTGC CGCTGAAGCC
    GAGAAGACTG CAGAAAGGGT TATCATCTGG GCTCGACTGG CACTGCAGGA GGAGCCTGAC
    GGCTCTGAGG AGGACAAGGC CTGGAGAATA TCTGTGGCGA AGACATCGGT GGATATTCTC
    AAGATCCTGT GTGATATTCG AAAAGACAAT CTGCAGAAGA AGGATGAATC TGAAGAATTC
    CTGAAGCGAT TTCAAATGGT TGCTAGTTTA CAGGAGAACT TTGAGGTCTT CCTTCCATTT
    GAAGACTACA GCAACACCGC CTTGGTAGCT GGCCTTCGAG AACAGTATAT TAAGGCACAG
    GAAGCTGCAC AGGCTGAGCA CAAACACAGG GGACCCCCCG GGCCCACCCC GGCTAGGGGG
    ACACACCTGA GCATCAAGTC AAAGCTGCAC CGACAGGCAC TGGCCCTGCA GGTGTCTGAA
    CAGGAGCTGG AGGCAGAGCT GACCTTGAGA GCCTTAAAGG ACGGGAAGGT GGTGGCCGCC
    CTGAGCAAAT GCAGAGATTT ACTTAAGCAT TACTGCAATG CCGACACTGG GAGGTTACTG
    TTTGTGGTGT GTCAGAAGCT CTGTCAGATG TTGGCTGACG ATGTCCCAAT GGTGGCCCCA
    GGAGGACTCA GCCTTCCTTC TGAGATCCAT GACCTCGCAT GCCACGCTGT CACCATCTGC
    AGTCCAGATT ATTTGCTGGA TGTTTTAGAA CTAAGTAAAT ACACTTTAAC CGCTGTGGAG
    CTTTGCAGAC AATGCCAGAT GGATGACTGT GGAATGCTGA TGAAGGCCGC TCTTGGAACT
    CACAAGGATC CCTATGAAGA GTGGTCCTTT AGTGACTTCT TCAGTGAAGA TGGAATTGTT
    CTCGAGTCCC AGGTGGTGCT GCCTGTCATC TATGAATTGA TCTCATCAGT GATGCCCCCT
    GCAGAAAGCA AAAGACATCC TTTGGATTCT ATAAGTTTGC CATACTGCTC CACTAGTGAA
    GGAGAAAACC GTATTCTCCC TCTTGTAAGC TCCATCTCTG CTCTGCTCCG GAGCCTCCAG
    GAATGTAGTC AGTGGGAGCT AGCCCTGAGG TTCGTGGTGG GCTCCTTTGG TACCTGCCTG
    CAGCACTCTA TGTCAAACGT CATGAGTATC AGCTTGAGTA AACAGTTACT TGGGAAGAAC
    ACATTGGCGA ACTCCAGGCA TATCATTATG GAATTGAAAG AGAAGTCCAT CACATTTATC
    AGAGAAAATG CTACGACACT CCTGCATAAA GTGTTTAACT GTCGGGTGGT GGATCTGGAC
    CTCGCCTTGG CTTACTGCAC TCTCTTACCT CAAAAGGATG TATTCGACAA CCTCTGGAAG
    TTCATAGATA AAGCGTGGCA GAATTACGAC AAAATTCTGG CATTATCTTT GGTGGGCTCT
    CAGCTGGCAA ATCTCTACCA GGATATTGAA ACAGGACTTT GGTTCCACGA GCTCAGTATC
    GATGCCAAGT GGGGTATTCG TCTTGGTAAA CTTGGTATTT CTTTTCAGCC TGCTTTTAGA
    CAAAATTTTC TCACCAAGAA AGACCTTATC AAAGCTCTTG TGAATAACAT AGACATGGAC
    ACCAGCCTCA TTCTGGAGTA TTGCAGCACA TTTCAGCTGG ACTCTGACGC TGCCCTTCGG
    CTGTTCATTG AAACTCTGCT CCGCAACACT AGCAGCCAGA GCCAGGGAGA TGCTGCCCCG
    GAGTCCACAA AGCATCAGCA TTCCAAACTC CTGGCGAAAG CCACTGAGCT GGTCCCTTTA
    CTGAAGAACA CAAAGGATCT GGTGATCAGT CTTAGTGAGA TCCTCTATAA GCTGGACCCA
    TATGATTATG AAATGATTGA TGTTGTCCTG AAAGTTTTAG AACAAGCTAA CGAAAAGATA
    ACCAGTGTGA ACATCAATCA GGCGCTGAAT CTTCTGAGAC ACTTGAAGTC ATACAGAAGG
    ATCTCCCCGC CAGTGGACCA CGAATACCAG TACGCGCTGG AGCACATGAT CACTCTGCCA
    CCGGCTGCTC ACACTAGACT GCCCTTTCAC CTAATACTGT TTGGCACAGC ACAGAACTTC
    TGGAAAATTC TCTCCTCAGA GCTCAGCGAA GAGTCGCTGC CAACCCTGCT CTTAATCGCG
    AAGCTGATGA AGTTCTCCCT AGATACTCTC TATGTGTCTA CCGCCAAACA CTTGTTTGAA
    AAAAACCTGA AGCCAAAGCT CCTGAAGTCG GCACAAGCCA GGTCCTCCAC CCTGATGAGC
    AAGGAAGTGG ATAAGCTCAT GCAGACGCTG GAGTCGTACC TCCTGTCCAT CGTCAACCCC
    GAGTGGGCTG TCGCCATTGC CATCAGCCTC ACCCAGGAAG TCCCAGAAGG CCCCTTCAAG
    ATGTCTAGTT TGAAGTTCTG CCTGTATTTA GCTGAGAGAT GGCTACAAAA CATCCCACCT
    CAGGATGAGA CGTGTGAAAA GGCCAAAGCC TTGCAGAAGA AGCTCTGCCT CCAGGTCCGG
    CTCTCAGGAA CGGAAGCCGT GCTCATAGCC CACAAGCTGA ACGATCAGGA ATATTTAAGA
    GTGATAGGGA AGCCAGCGCA CCTCATCGTG AGCCTCTACG AACATCCCAG CATAAGCGAA
    AGGCTTTGTA CCACATCTGG CAAAGATTAT CCCGATATCC ATACAGCCGC TAAAGAAATA
    GCCGAAGTCA ATGAAGTTAA TTTGGAAAAA ATCTGGGACA TGTTGTTGGA GAAATGGCTG
    TGCCCCTCAA CGGTGCCCAG TGAGAAAGCA TCGGAGTTCT TTGAGCTGGA AGAGGATGAA
    GTGCTGCATA GAGTCGTGTA TCTCCTCCAG GCTCGTCCTG TTGATTATTG TTCGAGAATG
    CTGTTTGTAT TTGCCACTTC AGCTACAAGT ACACTGGGCA TGCGCCAGCT AACGTTTGCC
    CACAAAGCAC GAGCTCTGCA GTGTCTCCTC TATTTGGCTG ACAAGGAAAC AATTGAATCT
    CTCTTCAAAA AGCCCATTAA AGAAATGAAA TCCTATCTGA AATGTATAAC TTTCCTGGCA
    TCATTTGAGA CATTGAATAT TCCCATCACA TATGAATTAT TCTGCAACAG TCCCAAAGAA
    GGCATGATTA AGGGCTTGTG GAAGAACCAC AGCCACGAAC CTATGGCAGT ACGATTGGTT
    GCAGAGCTTT GCTTAGAATA CAAAATCTAT GACCTGCAGC TTTGGAATGG GCTCTTGCAG
    AAGCTTCTGG GCTTCAACAT GATTCCTTAC CTGAGGAAAG TGTTATCCTG CATTTCTAGC
    ATCCATTCGT TATGGCAGGT GCCCTACTTC AGCAAAGCGT GGCAGCGCGT GATCCAGATC
    CCTCTGCTCT CGGCTTCCTG TCCTTTAAGA CCCAGTCAGT TAGCAGACTG CTGTGACAGT
    CTCGTCGCCA TCCTTGAATG TCCAGTTTCA GATGACCTGG ACATGATGGG AGTCGCCAAG
    CAGTACGTCC AGTTAGACCT TCCCGCTTTT GCTCTGACCT GCCTGACGCT CATGCCCCAC
    TCTGAAAAAA GACACCAGCA GATCAAGAAT TTTCTGAATT CCTGTGATGC TCGGATCATT
    TTACAGCAGA TAGAAGAACA TATGAACACT GGCCAGCTAG CAGGGTTTTC ACATCAGATT
    GGAAGTCTGG TTCTGAATCA TGTTGTAAAT AAGAAGGAAT TTGGGATTTT GGCAAAGACA
    AAGTATTTTC AGTTGTTGAA ATGCCACGTG ATAAACACCG GCAACGTCAC TGAATTGGTA
    AACTATTTGG CAAATGACTT CAGTGTAGAT GAAGCATCAG CGTTGATAAA TGAGTATTCA
    AAGCATTGTG GGAAGCCTGT GCCAGCAGAT GCAGCTCCCT GTGAGATCCT GCAGACATTT
    CTTGGTGGAT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006530314.1
    CDS103..4317
    Translation

    Target ORF information:

    RefSeq Version XM_006530251.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus kinetochore associated 1 (Kntc1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006530251.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTACAAGG CGGTGGTTCC CTGGAGTGCA GCCGTGGAGC AGCTGGTGAA ACAGCACCTG 
    GAAATGGACC ACCCCAAAGT CAAGTTATTG CAGGAAAGCT ACAAACTGAT GGAGATGAAG
    AAACTTTTGC GAGGGTATGG GATAAGAGAG GTGAATCTCC TGAACAAGGA AATAATGAGA
    GTGATCCGAT ACATTCTAAA GCAGGACATC CCGTCTTCTT TAGAAGATGC TCTGAAGGTG
    GCCCAGGGGT ACAGGCTGTC TGATGACGAA ATCTACAGCC TGAGAATCAT CGATCTGATT
    GACAGGGAGC AGGGGGGAGA TTGCCTCCTG CTACTGAAGT CTCTGCCTGC CGCTGAAGCC
    GAGAAGACTG CAGAAAGGGT TATCATCTGG GCTCGACTGG CACTGCAGGA GGAGCCTGAC
    GGCTCTGAGG AGGACAAGGC CTGGAGAATA TCTGTGGCGA AGACATCGGT GGATATTCTC
    AAGATCCTGT GTGATATTCG AAAAGACAAT CTGCAGAAGA AGGATGAATC TGAAGAATTC
    CTGAAGCGAT TTCAAATGGT TGCTAGTTTA CAGGAGAACT TTGAGGTCTT CCTTCCATTT
    GAAGACTACA GCAACACCGC CTTGGTAGCT GGCCTTCGAG AACAGTATAT TAAGGCACAG
    GAAGCTGCAC AGGCTGAGCA CAAACACAGG GGACCCCCCG GGCCCACCCC GGCTAGGGGG
    ACACACCTGA GCATCAAGTC AAAGCTGCAC CGACAGGCAC TGGCCCTGCA GGTGTCTGAA
    CAGGAGCTGG AGGCAGAGCT GACCTTGAGA GCCTTAAAGG ACGGGAAGGT GGTGGCCGCC
    CTGAGCAAAT GCAGAGATTT ACTTAAGCAT TACTGCAATG CCGACACTGG GAGGTTACTG
    TTTGTGGTGT GTCAGAAGCT CTGTCAGATG TTGGCTGACG ATGTCCCAAT GGTGGCCCCA
    GGAGGACTCA GCCTTCCTTC TGAGATCCAT GACCTCGCAT GCCACGCTGT CACCATCTGC
    AGTCCAGATT ATTTGCTGGA TGTTTTAGAA CTAAGTAAAT ACACTTTAAC CGCTGTGGAG
    CTTTGCAGAC AATGCCAGAT GGATGACTGT GGAATGCTGA TGAAGGCCGC TCTTGGAACT
    CACAAGGATC CCTATGAAGA GTGGTCCTTT AGTGACTTCT TCAGTGAAGA TGGAATTGTT
    CTCGAGTCCC AGGTGGTGCT GCCTGTCATC TATGAATTGA TCTCATCAGT GATGCCCCCT
    GCAGAAAGCA AAAGACATCC TTTGGATTCT ATAAGTTTGC CATACTGCTC CACTAGTGAA
    GGAGAAAACC GTATTCTCCC TCTTGTAAGC TCCATCTCTG CTCTGCTCCG GAGCCTCCAG
    GAATGTAGTC AGTGGGAGCT AGCCCTGAGG TTCGTGGTGG GCTCCTTTGG TACCTGCCTG
    CAGCACTCTA TGTCAAACGT CATGAGTATC AGCTTGAGTA AACAGTTACT TGGGAAGAAC
    ACATTGGCGA ACTCCAGGCA TATCATTATG GAATTGAAAG AGAAGTCCAT CACATTTATC
    AGAGAAAATG CTACGACACT CCTGCATAAA GTGTTTAACT GTCGGGTGGT GGATCTGGAC
    CTCGCCTTGG CTTACTGCAC TCTCTTACCT CAAAAGGATG TATTCGACAA CCTCTGGAAG
    TTCATAGATA AAGCGTGGCA GAATTACGAC AAAATTCTGG CATTATCTTT GGTGGGCTCT
    CAGCTGGCAA ATCTCTACCA GGATATTGAA ACAGGACTTT GGTTCCACGA GCTCAGTATC
    GATGCCAAGT GGGGTATTCG TCTTGGTAAA CTTGGTATTT CTTTTCAGCC TGCTTTTAGA
    CAAAATTTTC TCACCAAGAA AGACCTTATC AAAGCTCTTG TGAATAACAT AGACATGGAC
    ACCAGCCTCA TTCTGGAGTA TTGCAGCACA TTTCAGCTGG ACTCTGACGC TGCCCTTCGG
    CTGTTCATTG AAACTCTGCT CCGCAACACT AGCAGCCAGA GCCAGGGAGA TGCTGCCCCG
    GAGTCCACAA AGCATCAGCA TTCCAAACTC CTGGCGAAAG CCACTGAGCT GGTCCCTTTA
    CTGAAGAACA CAAAGGATCT GGTGATCAGT CTTAGTGAGA TCCTCTATAA GCTGGACCCA
    TATGATTATG AAATGATTGA TGTTGTCCTG AAAGTTTTAG AACAAGCTAA CGAAAAGATA
    ACCAGTGTGA ACATCAATCA GGCGCTGAAT CTTCTGAGAC ACTTGAAGTC ATACAGAAGG
    ATCTCCCCGC CAGTGGACCA CGAATACCAG TACGCGCTGG AGCACATGAT CACTCTGCCA
    CCGGCTGCTC ACACTAGACT GCCCTTTCAC CTAATACTGT TTGGCACAGC ACAGAACTTC
    TGGAAAATTC TCTCCTCAGA GCTCAGCGAA GAGTCGCTGC CAACCCTGCT CTTAATCGCG
    AAGCTGATGA AGTTCTCCCT AGATACTCTC TATGTGTCTA CCGCCAAACA CTTGTTTGAA
    AAAAACCTGA AGCCAAAGCT CCTGAAGTCG GCACAAGCCA GGTCCTCCAC CCTGATGAGC
    AAGGAAGTGG ATAAGCTCAT GCAGACGCTG GAGTCGTACC TCCTGTCCAT CGTCAACCCC
    GAGTGGGCTG TCGCCATTGC CATCAGCCTC ACCCAGGAAG TCCCAGAAGG CCCCTTCAAG
    ATGTCTAGTT TGAAGTTCTG CCTGTATTTA GCTGAGAGAT GGCTACAAAA CATCCCACCT
    CAGGATGAGA CGTGTGAAAA GGCCAAAGCC TTGCAGAAGA AGCTCTGCCT CCAGGTCCGG
    CTCTCAGGAA CGGAAGCCGT GCTCATAGCC CACAAGCTGA ACGATCAGGA ATATTTAAGA
    GTGATAGGGA AGCCAGCGCA CCTCATCGTG AGCCTCTACG AACATCCCAG CATAAGCGAA
    AGGCTTTGTA CCACATCTGG CAAAGATTAT CCCGATATCC ATACAGCCGC TAAAGAAATA
    GCCGAAGTCA ATGAAGTTAA TTTGGAAAAA ATCTGGGACA TGTTGTTGGA GAAATGGCTG
    TGCCCCTCAA CGGTGCCCAG TGAGAAAGCA TCGGAGTTCT TTGAGCTGGA AGAGGATGAA
    GTGCTGCATA GAGTCGTGTA TCTCCTCCAG GCTCGTCCTG TTGATTATTG TTCGAGAATG
    CTGTTTGTAT TTGCCACTTC AGCTACAAGT ACACTGGGCA TGCGCCAGCT AACGTTTGCC
    CACAAAGCAC GAGCTCTGCA GTGTCTCCTC TATTTGGCTG ACAAGGAAAC AATTGAATCT
    CTCTTCAAAA AGCCCATTAA AGAAATGAAA TCCTATCTGA AATGTATAAC TTTCCTGGCA
    TCATTTGAGA CATTGAATAT TCCCATCACA TATGAATTAT TCTGCAACAG TCCCAAAGAA
    GGCATGATTA AGGGCTTGTG GAAGAACCAC AGCCACGAAC CTATGGCAGT ACGATTGGTT
    GCAGAGCTTT GCTTAGAATA CAAAATCTAT GACCTGCAGC TTTGGAATGG GCTCTTGCAG
    AAGCTTCTGG GCTTCAACAT GATTCCTTAC CTGAGGAAAG TGTTATCCTG CATTTCTAGC
    ATCCATTCGT TATGGCAGGT GCCCTACTTC AGCAAAGCGT GGCAGCGCGT GATCCAGATC
    CCTCTGCTCT CGGCTTCCTG TCCTTTAAGA CCCAGTCAGT TAGCAGACTG CTGTGACAGT
    CTCGTCGCCA TCCTTGAATG TCCAGTTTCA GATGACCTGG ACATGATGGG AGTCGCCAAG
    CAGTACGTCC AGTTAGACCT TCCCGCTTTT GCTCTGACCT GCCTGACGCT CATGCCCCAC
    TCTGAAAAAA GACACCAGCA GATCAAGAAT TTTCTGAATT CCTGTGATGC TCGGATCATT
    TTACAGCAGA TAGAAGAACA TATGAACACT GGCCAGCTAG CAGGGTTTTC ACATCAGATT
    GGAAGTCTGG TTCTGAATCA TGTTGTAAAT AAGAAGGAAT TTGGGATTTT GGCAAAGACA
    AAGTATTTTC AGTTGTTGAA ATGCCACGTG ATAAACACCG GCAACGTCAC TGAATTGGTA
    AACTATTTGG CAAATGACTT CAGTGTAGAT GAAGCATCAG CGTTGATAAA TGAGTATTCA
    AAGCATTGTG GGAAGCCTGT GCCAGCAGAT GCAGCTCCCT GTGAGATCCT GCAGACATTT
    CTTGGTGGAT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35847
    Clone ID Related Accession (Same CDS sequence) XM_006530252.3 , XM_006530252.4
    Accession Version XM_006530252.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4170bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product kinetochore-associated protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530252.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGAACA ATATTGAGCT GCTAACAAGC GATGACACTG GAAGTGGATG CCTGAGTGTT 
    GGCTCAAGAA AAGAAAACGT GACTGCACTG TACCAAGCCG ACTTGCTTGG GAAGATCTCC
    TCTGAGAAGA CCTCACTGAG TCCGAAGATC CAAGCATTCA GCTTAAGCCA TGGGTTTATA
    ATCGTTGCAG ACCAGTCAGT GATATTGCTT GACAGTATAT GCAGATCACT CCAGTTGTTT
    CTTATCTTCG ATACTGACGT GGATGTGGTC GGCCTGTGTC AAGAAGGAAA GTTTCTCTTT
    GTGGGGGAGA GAAGCGGCAA CTTCCACCTT ATCTACGTCA CATCGAAGCA AACGCTGTTC
    ACCAAGGCTT TTGTGGAGAA AGCTTTGGAC GAGAGTCAGC GAACGTACCG GAATCTCATT
    ATTGAGAAAG ATGGTTCAAA TGAAGGTACC TATTATATGT TGCTTCTAAC GAACAATGGA
    TTTTTTTATA TTACAAACCT CCAGCTTTCA CAAATTGAAC AGGCCATTGA GAACACGGAC
    TTGGATTCAG CCAAGAAGTT ACAAGGACAA TTCAAATGTA GTTTTATTTC TACTGAAAAT
    TACCACAGTT GTCTCAGTCT TGTGGCATCT CAATCTGGAA CATTTGCAAG TAAAACTTCT
    GTGATAATTG GGGGAACCGG TAGTTGTGCA TTCTCAAAAT GGGAACCCGA TTCTACCAAG
    AAAGAAATGT CTCTTAAGAA CTTTGTGGGC ACAGACATTA TTAAAGGTGC AAAGTCATTT
    CAGCTGATCG ACAACCTGCT TTTTGTGCTG GATACTGATA ATGTGCTGAG CTTGTGGGAT
    GCTTACACTC TCACTCCTGT GTGGAACTGG CCCTCTCTTC CCGTAGAGCA GTTTGTTCTC
    ACGACAGAAG CGGATTCTCC CTCATCTGTT ACCTGGCAAG GAATTACAAA TCTCAAATTA
    GTCACCCTGA CAGCAACAGC GAAGGAGAAG ATGAGAAGCC TCATCATTTA CTCTCTGCCT
    TCGATGGAAA CACTGTACTC TTTGGAAGTA TCTAGTGTTT CTTCTTTAGT TCAAACAGGA
    ATCAGCACGG ACACTATATA TCTTCTGGAA GGAATTCACA AAAATGATCC TAACCTATGT
    GAAGACTCTG TGTCTGACTT AGTACTTAGG TACCTCACAG AGGTGTTACC AGAAAACAGA
    CTGAGCCGGT TACTTCACAA GCACAGATTT GCCGAAGCCG AGAGCTTTGC CATTCAGTTC
    GGACTGGATG TTGAGCTTGT TTATAAGGTC AAATCAAATG ATATGTTGGA GAAGCTGGCT
    TTGATTTCCT CGGACAAGAG TGAGCAGTCC AAATGGCAGC AACTTGTAGA TGAAGCTAAA
    GAAAACCTAT GCAAAATCCA GGACGATGAC TTCGTGGTGA ATTTCTGCCT CAAGGCCCAA
    TGGGTAACCT ATGAAACCAC CCAGGAAATG CTCAGTTATG CCAAAACCAG GCTCATGAAG
    AAAGAAGACA GAGCTCTACC TGCCTCTTCT GATGCCTTCA TGGAGGTGCT AAAAGCTCAT
    GCAAAGTTAA CTACATTTTA TGGAGCATTT GGACCAGAAA AATTCAGTGG TAGCTCTTGG
    ATTGAGTTCC TGAATAACGA AGATGACCTC AGGGATGTTT TTTTGCAGCT AAGTGAAGGA
    AACTTCGCTT GTGCTCAGTA TCTTTGGCTT AGACATCGGG CTGACTTTGA AAGCAAGTTT
    GACGTGAAAA TGCTGGAGAA TTTGCTGAAT TCAATATCTA CACAATTCCC CTTGGAAAAC
    CTTTGTTCCT GGTTTAAAAA TGAAGTGATT CCGTTTGTGA GGAGGATTGT GCCCGAAGGA
    CAAAACATTC TTGCAAAATG GCTAGAACAA GCATCAAGGA ACCTAGAGTT AACTGATAAG
    GCAAACTGGC CAGAAAATGG ACTTCAGCTG GCAGAGGTGT TCTTCACTGC AGAAAAAACA
    GACCGGTTTG GATTCGCATC TTCTTGGCAC TGGATTTCCT TGGATTATCA AAACACAGAG
    GAAGTCCGCC AGTTACGGAC ACTGGTGAGC AAGTTGCGGG AGCTGATCAT TCTGCATAGG
    AAATACAACT GCAAGCTGGC CCTCTCGGAC TTTGAGAAGG AAAATGCCAC CACTGTGGTG
    TTCCGCATGT TCGACAGGGT CTCGGCCCCA GAGCTGATCC CTTCGGTCTT AGAGAAGTCT
    GTGAGAGTTT ACATTCGCGA ACAGAATCTG CAAGAGGAGG AGCTGCTGTT GCTCTATATA
    GAGGATTTAC TGAAGAGGTG TAGCTCAAAG TCAATGACTC TCTTCGACAC AGCGTGGGAA
    GCAAAGGCCA TGGCCGTGAT AAGATGCTTG TCTGACACAG ATCTCATCTT TGATGCTGTG
    CTGAAGATAA TGTACAAGGC GGTGGTTCCC TGGAGTGCAG CCGTGGAGCA GCTGGTGAAA
    CAGCACCTGG AAATGGACCA CCCCAAAGTC AAGTTATTGC AGGAAAGCTA CAAACTGATG
    GAGATGAAGA AACTTTTGCG AGGGTATGGG ATAAGAGAGG TGAATCTCCT GAACAAGGAA
    ATAATGAGAG TGATCCGATA CATTCTAAAG CAGGACATCC CGTCTTCTTT AGAAGATGCT
    CTGAAGGTGG CCCAGGGGTA CAGGCTGTCT GATGACGAAA TCTACAGCCT GAGAATCATC
    GATCTGATTG ACAGGGAGCA GGGGGGAGAT TGCCTCCTGC TACTGAAGTC TCTGCCTGCC
    GCTGAAGCCG AGAAGACTGC AGAAAGGGTT ATCATCTGGG CTCGACTGGC ACTGCAGGAG
    GAGCCTGACG GCTCTGAGGA GGACAAGGCC TGGAGAATAT CTGTGGCGAA GACATCGGTG
    GATATTCTCA AGATCCTGTG TGATATTCGA AAAGACAATC TGCAGAAGAA GGATGAATCT
    GAAGAATTCC TGAAGCGATT TCAAATGGTT GCTAGTTTAC AGGAGAACTT TGAGGTCTTC
    CTTCCATTTG AAGACTACAG CAACACCGCC TTGGTAGCTG GCCTTCGAGA ACAGTATATT
    AAGGCACAGG AAGCTGCACA GGCTGAGCAC AAACACAGGG GACCCCCCGG GCCCACCCCG
    GCTAGGGGGA CACACCTGAG CATCAAGTCA AAGCTGCACC GACAGGCACT GGCCCTGCAG
    GTGTCTGAAC AGGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAGGA CGGGAAGGTG
    GTGGCCGCCC TGAGCAAATG CAGAGATTTA CTTAAGCATT ACTGCAATGC CGACACTGGG
    AGGTTACTGT TTGTGGTGTG TCAGAAGCTC TGTCAGATGT TGGCTGACGA TGTCCCAATG
    GTGGCCCCAG GAGGACTCAG CCTTCCTTCT GAGATCCATG ACCTCGCATG CCACGCTGTC
    ACCATCTGCA GTCCAGATTA TTTGCTGGAT GTTTTAGAAC TAAGTAAATA CACTTTAACC
    GCTGTGGAGC TTTGCAGACA ATGCCAGATG GATGACTGTG GAATGCTGAT GAAGGCCGCT
    CTTGGAACTC ACAAGGATCC CTATGAAGAG TGGTCCTTTA GTGACTTCTT CAGTGAAGAT
    GGAATTGTTC TCGAGTCCCA GGTGGTGCTG CCTGTCATCT ATGAATTGAT CTCATCAGTG
    ATGCCCCCTG CAGAAAGCAA AAGACATCCT TTGGATTCTA TAAGTTTGCC ATACTGCTCC
    ACTAGTGAAG GAGAAAACCG TATTCTCCCT CTTGTAAGCT CCATCTCTGC TCTGCTCCGG
    AGCCTCCAGG AATGTAGTCA GTGGGAGCTA GCCCTGAGGT TCGTGGTGGG CTCCTTTGGT
    ACCTGCCTGC AGCACTCTAT GTCAAACGTC ATGAGTATCA GCTTGAGTAA ACAGTTACTT
    GGGAAGAACA CATTGGCGAA CTCCAGGCAT ATCATTATGG AATTGAAAGA GAAGTCCATC
    ACATTTATCA GAGAAAATGC TACGACACTC CTGCATAAAG TGTTTAACTG TCGGGTGGTG
    GATCTGGACC TCGCCTTGGC TTACTGCACT CTCTTACCTC AAAAGGATGT ATTCGACAAC
    CTCTGGAAGT TCATAGATAA AGCGTGGCAG AATTACGACA AAATTCTGGT ACGCCCTGAG
    GGAGGAGACC TGCTCCTGTT GCCATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006530315.1
    CDS179..4348
    Translation

    Target ORF information:

    RefSeq Version XM_006530252.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus kinetochore associated 1 (Kntc1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006530252.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGAACA ATATTGAGCT GCTAACAAGC GATGACACTG GAAGTGGATG CCTGAGTGTT 
    GGCTCAAGAA AAGAAAACGT GACTGCACTG TACCAAGCCG ACTTGCTTGG GAAGATCTCC
    TCTGAGAAGA CCTCACTGAG TCCGAAGATC CAAGCATTCA GCTTAAGCCA TGGGTTTATA
    ATCGTTGCAG ACCAGTCAGT GATATTGCTT GACAGTATAT GCAGATCACT CCAGTTGTTT
    CTTATCTTCG ATACTGACGT GGATGTGGTC GGCCTGTGTC AAGAAGGAAA GTTTCTCTTT
    GTGGGGGAGA GAAGCGGCAA CTTCCACCTT ATCTACGTCA CATCGAAGCA AACGCTGTTC
    ACCAAGGCTT TTGTGGAGAA AGCTTTGGAC GAGAGTCAGC GAACGTACCG GAATCTCATT
    ATTGAGAAAG ATGGTTCAAA TGAAGGTACC TATTATATGT TGCTTCTAAC GAACAATGGA
    TTTTTTTATA TTACAAACCT CCAGCTTTCA CAAATTGAAC AGGCCATTGA GAACACGGAC
    TTGGATTCAG CCAAGAAGTT ACAAGGACAA TTCAAATGTA GTTTTATTTC TACTGAAAAT
    TACCACAGTT GTCTCAGTCT TGTGGCATCT CAATCTGGAA CATTTGCAAG TAAAACTTCT
    GTGATAATTG GGGGAACCGG TAGTTGTGCA TTCTCAAAAT GGGAACCCGA TTCTACCAAG
    AAAGAAATGT CTCTTAAGAA CTTTGTGGGC ACAGACATTA TTAAAGGTGC AAAGTCATTT
    CAGCTGATCG ACAACCTGCT TTTTGTGCTG GATACTGATA ATGTGCTGAG CTTGTGGGAT
    GCTTACACTC TCACTCCTGT GTGGAACTGG CCCTCTCTTC CCGTAGAGCA GTTTGTTCTC
    ACGACAGAAG CGGATTCTCC CTCATCTGTT ACCTGGCAAG GAATTACAAA TCTCAAATTA
    GTCACCCTGA CAGCAACAGC GAAGGAGAAG ATGAGAAGCC TCATCATTTA CTCTCTGCCT
    TCGATGGAAA CACTGTACTC TTTGGAAGTA TCTAGTGTTT CTTCTTTAGT TCAAACAGGA
    ATCAGCACGG ACACTATATA TCTTCTGGAA GGAATTCACA AAAATGATCC TAACCTATGT
    GAAGACTCTG TGTCTGACTT AGTACTTAGG TACCTCACAG AGGTGTTACC AGAAAACAGA
    CTGAGCCGGT TACTTCACAA GCACAGATTT GCCGAAGCCG AGAGCTTTGC CATTCAGTTC
    GGACTGGATG TTGAGCTTGT TTATAAGGTC AAATCAAATG ATATGTTGGA GAAGCTGGCT
    TTGATTTCCT CGGACAAGAG TGAGCAGTCC AAATGGCAGC AACTTGTAGA TGAAGCTAAA
    GAAAACCTAT GCAAAATCCA GGACGATGAC TTCGTGGTGA ATTTCTGCCT CAAGGCCCAA
    TGGGTAACCT ATGAAACCAC CCAGGAAATG CTCAGTTATG CCAAAACCAG GCTCATGAAG
    AAAGAAGACA GAGCTCTACC TGCCTCTTCT GATGCCTTCA TGGAGGTGCT AAAAGCTCAT
    GCAAAGTTAA CTACATTTTA TGGAGCATTT GGACCAGAAA AATTCAGTGG TAGCTCTTGG
    ATTGAGTTCC TGAATAACGA AGATGACCTC AGGGATGTTT TTTTGCAGCT AAGTGAAGGA
    AACTTCGCTT GTGCTCAGTA TCTTTGGCTT AGACATCGGG CTGACTTTGA AAGCAAGTTT
    GACGTGAAAA TGCTGGAGAA TTTGCTGAAT TCAATATCTA CACAATTCCC CTTGGAAAAC
    CTTTGTTCCT GGTTTAAAAA TGAAGTGATT CCGTTTGTGA GGAGGATTGT GCCCGAAGGA
    CAAAACATTC TTGCAAAATG GCTAGAACAA GCATCAAGGA ACCTAGAGTT AACTGATAAG
    GCAAACTGGC CAGAAAATGG ACTTCAGCTG GCAGAGGTGT TCTTCACTGC AGAAAAAACA
    GACCGGTTTG GATTCGCATC TTCTTGGCAC TGGATTTCCT TGGATTATCA AAACACAGAG
    GAAGTCCGCC AGTTACGGAC ACTGGTGAGC AAGTTGCGGG AGCTGATCAT TCTGCATAGG
    AAATACAACT GCAAGCTGGC CCTCTCGGAC TTTGAGAAGG AAAATGCCAC CACTGTGGTG
    TTCCGCATGT TCGACAGGGT CTCGGCCCCA GAGCTGATCC CTTCGGTCTT AGAGAAGTCT
    GTGAGAGTTT ACATTCGCGA ACAGAATCTG CAAGAGGAGG AGCTGCTGTT GCTCTATATA
    GAGGATTTAC TGAAGAGGTG TAGCTCAAAG TCAATGACTC TCTTCGACAC AGCGTGGGAA
    GCAAAGGCCA TGGCCGTGAT AAGATGCTTG TCTGACACAG ATCTCATCTT TGATGCTGTG
    CTGAAGATAA TGTACAAGGC GGTGGTTCCC TGGAGTGCAG CCGTGGAGCA GCTGGTGAAA
    CAGCACCTGG AAATGGACCA CCCCAAAGTC AAGTTATTGC AGGAAAGCTA CAAACTGATG
    GAGATGAAGA AACTTTTGCG AGGGTATGGG ATAAGAGAGG TGAATCTCCT GAACAAGGAA
    ATAATGAGAG TGATCCGATA CATTCTAAAG CAGGACATCC CGTCTTCTTT AGAAGATGCT
    CTGAAGGTGG CCCAGGGGTA CAGGCTGTCT GATGACGAAA TCTACAGCCT GAGAATCATC
    GATCTGATTG ACAGGGAGCA GGGGGGAGAT TGCCTCCTGC TACTGAAGTC TCTGCCTGCC
    GCTGAAGCCG AGAAGACTGC AGAAAGGGTT ATCATCTGGG CTCGACTGGC ACTGCAGGAG
    GAGCCTGACG GCTCTGAGGA GGACAAGGCC TGGAGAATAT CTGTGGCGAA GACATCGGTG
    GATATTCTCA AGATCCTGTG TGATATTCGA AAAGACAATC TGCAGAAGAA GGATGAATCT
    GAAGAATTCC TGAAGCGATT TCAAATGGTT GCTAGTTTAC AGGAGAACTT TGAGGTCTTC
    CTTCCATTTG AAGACTACAG CAACACCGCC TTGGTAGCTG GCCTTCGAGA ACAGTATATT
    AAGGCACAGG AAGCTGCACA GGCTGAGCAC AAACACAGGG GACCCCCCGG GCCCACCCCG
    GCTAGGGGGA CACACCTGAG CATCAAGTCA AAGCTGCACC GACAGGCACT GGCCCTGCAG
    GTGTCTGAAC AGGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAGGA CGGGAAGGTG
    GTGGCCGCCC TGAGCAAATG CAGAGATTTA CTTAAGCATT ACTGCAATGC CGACACTGGG
    AGGTTACTGT TTGTGGTGTG TCAGAAGCTC TGTCAGATGT TGGCTGACGA TGTCCCAATG
    GTGGCCCCAG GAGGACTCAG CCTTCCTTCT GAGATCCATG ACCTCGCATG CCACGCTGTC
    ACCATCTGCA GTCCAGATTA TTTGCTGGAT GTTTTAGAAC TAAGTAAATA CACTTTAACC
    GCTGTGGAGC TTTGCAGACA ATGCCAGATG GATGACTGTG GAATGCTGAT GAAGGCCGCT
    CTTGGAACTC ACAAGGATCC CTATGAAGAG TGGTCCTTTA GTGACTTCTT CAGTGAAGAT
    GGAATTGTTC TCGAGTCCCA GGTGGTGCTG CCTGTCATCT ATGAATTGAT CTCATCAGTG
    ATGCCCCCTG CAGAAAGCAA AAGACATCCT TTGGATTCTA TAAGTTTGCC ATACTGCTCC
    ACTAGTGAAG GAGAAAACCG TATTCTCCCT CTTGTAAGCT CCATCTCTGC TCTGCTCCGG
    AGCCTCCAGG AATGTAGTCA GTGGGAGCTA GCCCTGAGGT TCGTGGTGGG CTCCTTTGGT
    ACCTGCCTGC AGCACTCTAT GTCAAACGTC ATGAGTATCA GCTTGAGTAA ACAGTTACTT
    GGGAAGAACA CATTGGCGAA CTCCAGGCAT ATCATTATGG AATTGAAAGA GAAGTCCATC
    ACATTTATCA GAGAAAATGC TACGACACTC CTGCATAAAG TGTTTAACTG TCGGGTGGTG
    GATCTGGACC TCGCCTTGGC TTACTGCACT CTCTTACCTC AAAAGGATGT ATTCGACAAC
    CTCTGGAAGT TCATAGATAA AGCGTGGCAG AATTACGACA AAATTCTGGT ACGCCCTGAG
    GGAGGAGACC TGCTCCTGTT GCCATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35848
    Clone ID Related Accession (Same CDS sequence) XM_006530253.3 , XM_006530253.4
    Accession Version XM_006530253.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3924bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product kinetochore-associated protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530253.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTGGAACA ATATTGAGCT GCTAACAAGC GATGACACTG GAAGTGGATG CCTGAGTGTT 
    GGCTCAAGAA AAGAAAACGT GACTGCACTG TACCAAGCCG ACTTGCTTGG GAAGATCTCC
    TCTGAGAAGA CCTCACTGAG TCCGAAGATC CAAGCATTCA GCTTAAGCCA TGGGTTTATA
    ATCGTTGCAG ACCAGTCAGT GATATTGCTT GACAGTATAT GCAGATCACT CCAGTTGTTT
    CTTATCTTCG ATACTGACGT GGATGTGGTC GGCCTGTGTC AAGAAGGAAA GTTTCTCTTT
    GTGGGGGAGA GAAGCGGCAA CTTCCACCTT ATCTACGTCA CATCGAAGCA AACGCTGTTC
    ACCAAGGCTT TTGTGGAGAA AGCTTTGGAC GAGAGTCAGC GAACGTACCG GAATCTCATT
    ATTGAGAAAG ATGGTTCAAA TGAAGGTACC TATTATATGT TGCTTCTAAC GAACAATGGA
    TTTTTTTATA TTACAAACCT CCAGCTTTCA CAAATTGAAC AGGCCATTGA GAACACGGAC
    TTGGATTCAG CCAAGAAGTT ACAAGGACAA TTCAAATGTA GTTTTATTTC TACTGAAAAT
    TACCACAGTT GTCTCAGTCT TGTGGCATCT CAATCTGGAA CATTTGCAAG TAAAACTTCT
    GTGATAATTG GGGGAACCGG TAGTTGTGCA TTCTCAAAAT GGGAACCCGA TTCTACCAAG
    AAAGAAATGT CTCTTAAGAA CTTTGTGGGC ACAGACATTA TTAAAGGTGC AAAGTCATTT
    CAGCTGATCG ACAACCTGCT TTTTGTGCTG GATACTGATA ATGTGCTGAG CTTGTGGGAT
    GCTTACACTC TCACTCCTGT GTGGAACTGG CCCTCTCTTC CCGTAGAGCA GTTTGTTCTC
    ACGACAGAAG CGGATTCTCC CTCATCTGTT ACCTGGCAAG GAATTACAAA TCTCAAATTA
    GTCACCCTGA CAGCAACAGC GAAGGAGAAG ATGAGAAGCC TCATCATTTA CTCTCTGCCT
    TCGATGGAAA CACTGTACTC TTTGGAAGTA TCTAGTGTTT CTTCTTTAGT TCAAACAGGA
    ATCAGCACGG ACACTATATA TCTTCTGGAA GGAATTCACA AAAATGATCC TAACCTATGT
    GAAGACTCTG TGTCTGACTT AGTACTTAGG TACCTCACAG AGGTGTTACC AGAAAACAGA
    CTGAGCCGGT TACTTCACAA GCACAGATTT GCCGAAGCCG AGAGCTTTGC CATTCAGTTC
    GGACTGGATG TTGAGCTTGT TTATAAGGTC AAATCAAATG ATATGTTGGA GAAGCTGGCT
    TTGATTTCCT CGGACAAGAG TGAGCAGTCC AAATGGCAGC AACTTGTAGA TGAAGCTAAA
    GAAAACCTAT GCAAAATCCA GGACGATGAC TTCGTGGTGA ATTTCTGCCT CAAGGCCCAA
    TGGGTAACCT ATGAAACCAC CCAGGAAATG CTCAGTTATG CCAAAACCAG GCTCATGAAG
    AAAGAAGACA GAGCTCTACC TGCCTCTTCT GATGCCTTCA TGGAGGTGCT AAAAGCTCAT
    GCAAAGTTAA CTACATTTTA TGGAGCATTT GGACCAGAAA AATTCAGTGG TAGCTCTTGG
    ATTGAGTTCC TGAATAACGA AGATGACCTC AGGGATGTTT TTTTGCAGCT AAGTGAAGGA
    AACTTCGCTT GTGCTCAGTA TCTTTGGCTT AGACATCGGG CTGACTTTGA AAGCAAGTTT
    GACGTGAAAA TGCTGGAGAA TTTGCTGAAT TCAATATCTA CACAATTCCC CTTGGAAAAC
    CTTTGTTCCT GGTTTAAAAA TGAAGTGATT CCGTTTGTGA GGAGGATTGT GCCCGAAGGA
    CAAAACATTC TTGCAAAATG GCTAGAACAA GCATCAAGGA ACCTAGAGTT AACTGATAAG
    GCAAACTGGC CAGAAAATGG ACTTCAGCTG GCAGAGGTGT TCTTCACTGC AGAAAAAACA
    GACCGGTTTG GATTCGCATC TTCTTGGCAC TGGATTTCCT TGGATTATCA AAACACAGAG
    GAAGTCCGCC AGTTACGGAC ACTGGTGAGC AAGTTGCGGG AGCTGATCAT TCTGCATAGG
    AAATACAACT GCAAGCTGGC CCTCTCGGAC TTTGAGAAGG AAAATGCCAC CACTGTGGTG
    TTCCGCATGT TCGACAGGGT CTCGGCCCCA GAGCTGATCC CTTCGGTCTT AGAGAAGTCT
    GTGAGAGTTT ACATTCGCGA ACAGAATCTG CAAGAGGAGG AGCTGCTGTT GCTCTATATA
    GAGGATTTAC TGAAGAGGTG TAGCTCAAAG TCAATGACTC TCTTCGACAC AGCGTGGGAA
    GCAAAGGCCA TGGCCGTGAT AAGATGCTTG TCTGACACAG ATCTCATCTT TGATGCTGTG
    CTGAAGATAA TGTACAAGGC GGTGGTTCCC TGGAGTGCAG CCGTGGAGCA GCTGGTGAAA
    CAGCACCTGG AAATGGACCA CCCCAAAGTC AAGTTATTGC AGGAAAGCTA CAAACTGATG
    GAGATGAAGA AACTTTTGCG AGGGTATGGG ATAAGAGAGG TGAATCTCCT GAACAAGGAA
    ATAATGAGAG TGATCCGATA CATTCTAAAG CAGGACATCC CGTCTTCTTT AGAAGATGCT
    CTGAAGGTGG CCCAGGGGTA CAGGCTGTCT GATGACGAAA TCTACAGCCT GAGAATCATC
    GATCTGATTG ACAGGGAGCA GGGGGGAGAT TGCCTCCTGC TACTGAAGTC TCTGCCTGCC
    GCTGAAGCCG AGAAGACTGC AGAAAGGGTT ATCATCTGGG CTCGACTGGC ACTGCAGGAG
    GAGCCTGACG GCTCTGAGGA GGACAAGGCC TGGAGAATAT CTGTGGCGAA GACATCGGTG
    GATATTCTCA AGATCCTGTG TGATATTCGA AAAGACAATC TGCAGAAGAA GGATGAATCT
    GAAGAATTCC TGAAGCGATT TCAAATGGTT GCTAGTTTAC AGGAGAACTT TGAGGTCTTC
    CTTCCATTTG AAGACTACAG CAACACCGCC TTGGTAGCTG GCCTTCGAGA ACAGTATATT
    AAGGCACAGG AAGCTGCACA GGCTGAGCAC AAACACAGGG GACCCCCCGG GCCCACCCCG
    GCTAGGGGGA CACACCTGAG CATCAAGTCA AAGCTGCACC GACAGGCACT GGCCCTGCAG
    GTGTCTGAAC AGGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAGGA CGGGAAGGTG
    GTGGCCGCCC TGAGCAAATG CAGAGATTTA CTTAAGCATT ACTGCAATGC CGACACTGGG
    AGGTTACTGT TTGTGGTGTG TCAGAAGCTC TGTCAGATGT TGGCTGACGA TGTCCCAATG
    GTGGCCCCAG GAGGACTCAG CCTTCCTTCT GAGATCCATG ACCTCGCATG CCACGCTGTC
    ACCATCTGCA GTCCAGATTA TTTGCTGGAT GTTTTAGAAC TAAGTAAATA CACTTTAACC
    GCTGTGGAGC TTTGCAGACA ATGCCAGATG GATGACTGTG GAATGCTGAT GAAGGCCGCT
    CTTGGAACTC ACAAGGATCC CTATGAAGAG TGGTCCTTTA GTGACTTCTT CAGTGAAGAT
    GGAATTGTTC TCGAGTCCCA GGTGGTGCTG CCTGTCATCT ATGAATTGAT CTCATCAGTG
    ATGCCCCCTG CAGAAAGCAA AAGACATCCT TTGGATTCTA TAAGTTTGCC ATACTGCTCC
    ACTAGTGAAG GAGAAAACCG TATTCTCCCT CTTGTAAGCT CCATCTCTGC TCTGCTCCGG
    AGCCTCCAGG AATGTAGTCA GTGGGAGCTA GCCCTGAGGT TCGTGGTGGG CTCCTTTGGT
    ACCTGCCTGC AGCACTCTAT GTCAAACGTC ATGAGTATCA GCTTGAGTAA ACAGAGATCC
    ACCTGCCTCT GTCTCCCGAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006530316.1
    CDS180..4103
    Translation

    Target ORF information:

    RefSeq Version XM_006530253.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus kinetochore associated 1 (Kntc1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006530253.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTGGAACA ATATTGAGCT GCTAACAAGC GATGACACTG GAAGTGGATG CCTGAGTGTT 
    GGCTCAAGAA AAGAAAACGT GACTGCACTG TACCAAGCCG ACTTGCTTGG GAAGATCTCC
    TCTGAGAAGA CCTCACTGAG TCCGAAGATC CAAGCATTCA GCTTAAGCCA TGGGTTTATA
    ATCGTTGCAG ACCAGTCAGT GATATTGCTT GACAGTATAT GCAGATCACT CCAGTTGTTT
    CTTATCTTCG ATACTGACGT GGATGTGGTC GGCCTGTGTC AAGAAGGAAA GTTTCTCTTT
    GTGGGGGAGA GAAGCGGCAA CTTCCACCTT ATCTACGTCA CATCGAAGCA AACGCTGTTC
    ACCAAGGCTT TTGTGGAGAA AGCTTTGGAC GAGAGTCAGC GAACGTACCG GAATCTCATT
    ATTGAGAAAG ATGGTTCAAA TGAAGGTACC TATTATATGT TGCTTCTAAC GAACAATGGA
    TTTTTTTATA TTACAAACCT CCAGCTTTCA CAAATTGAAC AGGCCATTGA GAACACGGAC
    TTGGATTCAG CCAAGAAGTT ACAAGGACAA TTCAAATGTA GTTTTATTTC TACTGAAAAT
    TACCACAGTT GTCTCAGTCT TGTGGCATCT CAATCTGGAA CATTTGCAAG TAAAACTTCT
    GTGATAATTG GGGGAACCGG TAGTTGTGCA TTCTCAAAAT GGGAACCCGA TTCTACCAAG
    AAAGAAATGT CTCTTAAGAA CTTTGTGGGC ACAGACATTA TTAAAGGTGC AAAGTCATTT
    CAGCTGATCG ACAACCTGCT TTTTGTGCTG GATACTGATA ATGTGCTGAG CTTGTGGGAT
    GCTTACACTC TCACTCCTGT GTGGAACTGG CCCTCTCTTC CCGTAGAGCA GTTTGTTCTC
    ACGACAGAAG CGGATTCTCC CTCATCTGTT ACCTGGCAAG GAATTACAAA TCTCAAATTA
    GTCACCCTGA CAGCAACAGC GAAGGAGAAG ATGAGAAGCC TCATCATTTA CTCTCTGCCT
    TCGATGGAAA CACTGTACTC TTTGGAAGTA TCTAGTGTTT CTTCTTTAGT TCAAACAGGA
    ATCAGCACGG ACACTATATA TCTTCTGGAA GGAATTCACA AAAATGATCC TAACCTATGT
    GAAGACTCTG TGTCTGACTT AGTACTTAGG TACCTCACAG AGGTGTTACC AGAAAACAGA
    CTGAGCCGGT TACTTCACAA GCACAGATTT GCCGAAGCCG AGAGCTTTGC CATTCAGTTC
    GGACTGGATG TTGAGCTTGT TTATAAGGTC AAATCAAATG ATATGTTGGA GAAGCTGGCT
    TTGATTTCCT CGGACAAGAG TGAGCAGTCC AAATGGCAGC AACTTGTAGA TGAAGCTAAA
    GAAAACCTAT GCAAAATCCA GGACGATGAC TTCGTGGTGA ATTTCTGCCT CAAGGCCCAA
    TGGGTAACCT ATGAAACCAC CCAGGAAATG CTCAGTTATG CCAAAACCAG GCTCATGAAG
    AAAGAAGACA GAGCTCTACC TGCCTCTTCT GATGCCTTCA TGGAGGTGCT AAAAGCTCAT
    GCAAAGTTAA CTACATTTTA TGGAGCATTT GGACCAGAAA AATTCAGTGG TAGCTCTTGG
    ATTGAGTTCC TGAATAACGA AGATGACCTC AGGGATGTTT TTTTGCAGCT AAGTGAAGGA
    AACTTCGCTT GTGCTCAGTA TCTTTGGCTT AGACATCGGG CTGACTTTGA AAGCAAGTTT
    GACGTGAAAA TGCTGGAGAA TTTGCTGAAT TCAATATCTA CACAATTCCC CTTGGAAAAC
    CTTTGTTCCT GGTTTAAAAA TGAAGTGATT CCGTTTGTGA GGAGGATTGT GCCCGAAGGA
    CAAAACATTC TTGCAAAATG GCTAGAACAA GCATCAAGGA ACCTAGAGTT AACTGATAAG
    GCAAACTGGC CAGAAAATGG ACTTCAGCTG GCAGAGGTGT TCTTCACTGC AGAAAAAACA
    GACCGGTTTG GATTCGCATC TTCTTGGCAC TGGATTTCCT TGGATTATCA AAACACAGAG
    GAAGTCCGCC AGTTACGGAC ACTGGTGAGC AAGTTGCGGG AGCTGATCAT TCTGCATAGG
    AAATACAACT GCAAGCTGGC CCTCTCGGAC TTTGAGAAGG AAAATGCCAC CACTGTGGTG
    TTCCGCATGT TCGACAGGGT CTCGGCCCCA GAGCTGATCC CTTCGGTCTT AGAGAAGTCT
    GTGAGAGTTT ACATTCGCGA ACAGAATCTG CAAGAGGAGG AGCTGCTGTT GCTCTATATA
    GAGGATTTAC TGAAGAGGTG TAGCTCAAAG TCAATGACTC TCTTCGACAC AGCGTGGGAA
    GCAAAGGCCA TGGCCGTGAT AAGATGCTTG TCTGACACAG ATCTCATCTT TGATGCTGTG
    CTGAAGATAA TGTACAAGGC GGTGGTTCCC TGGAGTGCAG CCGTGGAGCA GCTGGTGAAA
    CAGCACCTGG AAATGGACCA CCCCAAAGTC AAGTTATTGC AGGAAAGCTA CAAACTGATG
    GAGATGAAGA AACTTTTGCG AGGGTATGGG ATAAGAGAGG TGAATCTCCT GAACAAGGAA
    ATAATGAGAG TGATCCGATA CATTCTAAAG CAGGACATCC CGTCTTCTTT AGAAGATGCT
    CTGAAGGTGG CCCAGGGGTA CAGGCTGTCT GATGACGAAA TCTACAGCCT GAGAATCATC
    GATCTGATTG ACAGGGAGCA GGGGGGAGAT TGCCTCCTGC TACTGAAGTC TCTGCCTGCC
    GCTGAAGCCG AGAAGACTGC AGAAAGGGTT ATCATCTGGG CTCGACTGGC ACTGCAGGAG
    GAGCCTGACG GCTCTGAGGA GGACAAGGCC TGGAGAATAT CTGTGGCGAA GACATCGGTG
    GATATTCTCA AGATCCTGTG TGATATTCGA AAAGACAATC TGCAGAAGAA GGATGAATCT
    GAAGAATTCC TGAAGCGATT TCAAATGGTT GCTAGTTTAC AGGAGAACTT TGAGGTCTTC
    CTTCCATTTG AAGACTACAG CAACACCGCC TTGGTAGCTG GCCTTCGAGA ACAGTATATT
    AAGGCACAGG AAGCTGCACA GGCTGAGCAC AAACACAGGG GACCCCCCGG GCCCACCCCG
    GCTAGGGGGA CACACCTGAG CATCAAGTCA AAGCTGCACC GACAGGCACT GGCCCTGCAG
    GTGTCTGAAC AGGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAGGA CGGGAAGGTG
    GTGGCCGCCC TGAGCAAATG CAGAGATTTA CTTAAGCATT ACTGCAATGC CGACACTGGG
    AGGTTACTGT TTGTGGTGTG TCAGAAGCTC TGTCAGATGT TGGCTGACGA TGTCCCAATG
    GTGGCCCCAG GAGGACTCAG CCTTCCTTCT GAGATCCATG ACCTCGCATG CCACGCTGTC
    ACCATCTGCA GTCCAGATTA TTTGCTGGAT GTTTTAGAAC TAAGTAAATA CACTTTAACC
    GCTGTGGAGC TTTGCAGACA ATGCCAGATG GATGACTGTG GAATGCTGAT GAAGGCCGCT
    CTTGGAACTC ACAAGGATCC CTATGAAGAG TGGTCCTTTA GTGACTTCTT CAGTGAAGAT
    GGAATTGTTC TCGAGTCCCA GGTGGTGCTG CCTGTCATCT ATGAATTGAT CTCATCAGTG
    ATGCCCCCTG CAGAAAGCAA AAGACATCCT TTGGATTCTA TAAGTTTGCC ATACTGCTCC
    ACTAGTGAAG GAGAAAACCG TATTCTCCCT CTTGTAAGCT CCATCTCTGC TCTGCTCCGG
    AGCCTCCAGG AATGTAGTCA GTGGGAGCTA GCCCTGAGGT TCGTGGTGGG CTCCTTTGGT
    ACCTGCCTGC AGCACTCTAT GTCAAACGTC ATGAGTATCA GCTTGAGTAA ACAGAGATCC
    ACCTGCCTCT GTCTCCCGAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35848
    Clone ID Related Accession (Same CDS sequence) XM_006530253.3 , XM_006530253.4
    Accession Version XM_006530253.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3924bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product kinetochore-associated protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530253.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTGGAACA ATATTGAGCT GCTAACAAGC GATGACACTG GAAGTGGATG CCTGAGTGTT 
    GGCTCAAGAA AAGAAAACGT GACTGCACTG TACCAAGCCG ACTTGCTTGG GAAGATCTCC
    TCTGAGAAGA CCTCACTGAG TCCGAAGATC CAAGCATTCA GCTTAAGCCA TGGGTTTATA
    ATCGTTGCAG ACCAGTCAGT GATATTGCTT GACAGTATAT GCAGATCACT CCAGTTGTTT
    CTTATCTTCG ATACTGACGT GGATGTGGTC GGCCTGTGTC AAGAAGGAAA GTTTCTCTTT
    GTGGGGGAGA GAAGCGGCAA CTTCCACCTT ATCTACGTCA CATCGAAGCA AACGCTGTTC
    ACCAAGGCTT TTGTGGAGAA AGCTTTGGAC GAGAGTCAGC GAACGTACCG GAATCTCATT
    ATTGAGAAAG ATGGTTCAAA TGAAGGTACC TATTATATGT TGCTTCTAAC GAACAATGGA
    TTTTTTTATA TTACAAACCT CCAGCTTTCA CAAATTGAAC AGGCCATTGA GAACACGGAC
    TTGGATTCAG CCAAGAAGTT ACAAGGACAA TTCAAATGTA GTTTTATTTC TACTGAAAAT
    TACCACAGTT GTCTCAGTCT TGTGGCATCT CAATCTGGAA CATTTGCAAG TAAAACTTCT
    GTGATAATTG GGGGAACCGG TAGTTGTGCA TTCTCAAAAT GGGAACCCGA TTCTACCAAG
    AAAGAAATGT CTCTTAAGAA CTTTGTGGGC ACAGACATTA TTAAAGGTGC AAAGTCATTT
    CAGCTGATCG ACAACCTGCT TTTTGTGCTG GATACTGATA ATGTGCTGAG CTTGTGGGAT
    GCTTACACTC TCACTCCTGT GTGGAACTGG CCCTCTCTTC CCGTAGAGCA GTTTGTTCTC
    ACGACAGAAG CGGATTCTCC CTCATCTGTT ACCTGGCAAG GAATTACAAA TCTCAAATTA
    GTCACCCTGA CAGCAACAGC GAAGGAGAAG ATGAGAAGCC TCATCATTTA CTCTCTGCCT
    TCGATGGAAA CACTGTACTC TTTGGAAGTA TCTAGTGTTT CTTCTTTAGT TCAAACAGGA
    ATCAGCACGG ACACTATATA TCTTCTGGAA GGAATTCACA AAAATGATCC TAACCTATGT
    GAAGACTCTG TGTCTGACTT AGTACTTAGG TACCTCACAG AGGTGTTACC AGAAAACAGA
    CTGAGCCGGT TACTTCACAA GCACAGATTT GCCGAAGCCG AGAGCTTTGC CATTCAGTTC
    GGACTGGATG TTGAGCTTGT TTATAAGGTC AAATCAAATG ATATGTTGGA GAAGCTGGCT
    TTGATTTCCT CGGACAAGAG TGAGCAGTCC AAATGGCAGC AACTTGTAGA TGAAGCTAAA
    GAAAACCTAT GCAAAATCCA GGACGATGAC TTCGTGGTGA ATTTCTGCCT CAAGGCCCAA
    TGGGTAACCT ATGAAACCAC CCAGGAAATG CTCAGTTATG CCAAAACCAG GCTCATGAAG
    AAAGAAGACA GAGCTCTACC TGCCTCTTCT GATGCCTTCA TGGAGGTGCT AAAAGCTCAT
    GCAAAGTTAA CTACATTTTA TGGAGCATTT GGACCAGAAA AATTCAGTGG TAGCTCTTGG
    ATTGAGTTCC TGAATAACGA AGATGACCTC AGGGATGTTT TTTTGCAGCT AAGTGAAGGA
    AACTTCGCTT GTGCTCAGTA TCTTTGGCTT AGACATCGGG CTGACTTTGA AAGCAAGTTT
    GACGTGAAAA TGCTGGAGAA TTTGCTGAAT TCAATATCTA CACAATTCCC CTTGGAAAAC
    CTTTGTTCCT GGTTTAAAAA TGAAGTGATT CCGTTTGTGA GGAGGATTGT GCCCGAAGGA
    CAAAACATTC TTGCAAAATG GCTAGAACAA GCATCAAGGA ACCTAGAGTT AACTGATAAG
    GCAAACTGGC CAGAAAATGG ACTTCAGCTG GCAGAGGTGT TCTTCACTGC AGAAAAAACA
    GACCGGTTTG GATTCGCATC TTCTTGGCAC TGGATTTCCT TGGATTATCA AAACACAGAG
    GAAGTCCGCC AGTTACGGAC ACTGGTGAGC AAGTTGCGGG AGCTGATCAT TCTGCATAGG
    AAATACAACT GCAAGCTGGC CCTCTCGGAC TTTGAGAAGG AAAATGCCAC CACTGTGGTG
    TTCCGCATGT TCGACAGGGT CTCGGCCCCA GAGCTGATCC CTTCGGTCTT AGAGAAGTCT
    GTGAGAGTTT ACATTCGCGA ACAGAATCTG CAAGAGGAGG AGCTGCTGTT GCTCTATATA
    GAGGATTTAC TGAAGAGGTG TAGCTCAAAG TCAATGACTC TCTTCGACAC AGCGTGGGAA
    GCAAAGGCCA TGGCCGTGAT AAGATGCTTG TCTGACACAG ATCTCATCTT TGATGCTGTG
    CTGAAGATAA TGTACAAGGC GGTGGTTCCC TGGAGTGCAG CCGTGGAGCA GCTGGTGAAA
    CAGCACCTGG AAATGGACCA CCCCAAAGTC AAGTTATTGC AGGAAAGCTA CAAACTGATG
    GAGATGAAGA AACTTTTGCG AGGGTATGGG ATAAGAGAGG TGAATCTCCT GAACAAGGAA
    ATAATGAGAG TGATCCGATA CATTCTAAAG CAGGACATCC CGTCTTCTTT AGAAGATGCT
    CTGAAGGTGG CCCAGGGGTA CAGGCTGTCT GATGACGAAA TCTACAGCCT GAGAATCATC
    GATCTGATTG ACAGGGAGCA GGGGGGAGAT TGCCTCCTGC TACTGAAGTC TCTGCCTGCC
    GCTGAAGCCG AGAAGACTGC AGAAAGGGTT ATCATCTGGG CTCGACTGGC ACTGCAGGAG
    GAGCCTGACG GCTCTGAGGA GGACAAGGCC TGGAGAATAT CTGTGGCGAA GACATCGGTG
    GATATTCTCA AGATCCTGTG TGATATTCGA AAAGACAATC TGCAGAAGAA GGATGAATCT
    GAAGAATTCC TGAAGCGATT TCAAATGGTT GCTAGTTTAC AGGAGAACTT TGAGGTCTTC
    CTTCCATTTG AAGACTACAG CAACACCGCC TTGGTAGCTG GCCTTCGAGA ACAGTATATT
    AAGGCACAGG AAGCTGCACA GGCTGAGCAC AAACACAGGG GACCCCCCGG GCCCACCCCG
    GCTAGGGGGA CACACCTGAG CATCAAGTCA AAGCTGCACC GACAGGCACT GGCCCTGCAG
    GTGTCTGAAC AGGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAGGA CGGGAAGGTG
    GTGGCCGCCC TGAGCAAATG CAGAGATTTA CTTAAGCATT ACTGCAATGC CGACACTGGG
    AGGTTACTGT TTGTGGTGTG TCAGAAGCTC TGTCAGATGT TGGCTGACGA TGTCCCAATG
    GTGGCCCCAG GAGGACTCAG CCTTCCTTCT GAGATCCATG ACCTCGCATG CCACGCTGTC
    ACCATCTGCA GTCCAGATTA TTTGCTGGAT GTTTTAGAAC TAAGTAAATA CACTTTAACC
    GCTGTGGAGC TTTGCAGACA ATGCCAGATG GATGACTGTG GAATGCTGAT GAAGGCCGCT
    CTTGGAACTC ACAAGGATCC CTATGAAGAG TGGTCCTTTA GTGACTTCTT CAGTGAAGAT
    GGAATTGTTC TCGAGTCCCA GGTGGTGCTG CCTGTCATCT ATGAATTGAT CTCATCAGTG
    ATGCCCCCTG CAGAAAGCAA AAGACATCCT TTGGATTCTA TAAGTTTGCC ATACTGCTCC
    ACTAGTGAAG GAGAAAACCG TATTCTCCCT CTTGTAAGCT CCATCTCTGC TCTGCTCCGG
    AGCCTCCAGG AATGTAGTCA GTGGGAGCTA GCCCTGAGGT TCGTGGTGGG CTCCTTTGGT
    ACCTGCCTGC AGCACTCTAT GTCAAACGTC ATGAGTATCA GCTTGAGTAA ACAGAGATCC
    ACCTGCCTCT GTCTCCCGAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006530316.1
    CDS182..4105
    Translation

    Target ORF information:

    RefSeq Version XM_006530253.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus kinetochore associated 1 (Kntc1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006530253.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGTGGAACA ATATTGAGCT GCTAACAAGC GATGACACTG GAAGTGGATG CCTGAGTGTT 
    GGCTCAAGAA AAGAAAACGT GACTGCACTG TACCAAGCCG ACTTGCTTGG GAAGATCTCC
    TCTGAGAAGA CCTCACTGAG TCCGAAGATC CAAGCATTCA GCTTAAGCCA TGGGTTTATA
    ATCGTTGCAG ACCAGTCAGT GATATTGCTT GACAGTATAT GCAGATCACT CCAGTTGTTT
    CTTATCTTCG ATACTGACGT GGATGTGGTC GGCCTGTGTC AAGAAGGAAA GTTTCTCTTT
    GTGGGGGAGA GAAGCGGCAA CTTCCACCTT ATCTACGTCA CATCGAAGCA AACGCTGTTC
    ACCAAGGCTT TTGTGGAGAA AGCTTTGGAC GAGAGTCAGC GAACGTACCG GAATCTCATT
    ATTGAGAAAG ATGGTTCAAA TGAAGGTACC TATTATATGT TGCTTCTAAC GAACAATGGA
    TTTTTTTATA TTACAAACCT CCAGCTTTCA CAAATTGAAC AGGCCATTGA GAACACGGAC
    TTGGATTCAG CCAAGAAGTT ACAAGGACAA TTCAAATGTA GTTTTATTTC TACTGAAAAT
    TACCACAGTT GTCTCAGTCT TGTGGCATCT CAATCTGGAA CATTTGCAAG TAAAACTTCT
    GTGATAATTG GGGGAACCGG TAGTTGTGCA TTCTCAAAAT GGGAACCCGA TTCTACCAAG
    AAAGAAATGT CTCTTAAGAA CTTTGTGGGC ACAGACATTA TTAAAGGTGC AAAGTCATTT
    CAGCTGATCG ACAACCTGCT TTTTGTGCTG GATACTGATA ATGTGCTGAG CTTGTGGGAT
    GCTTACACTC TCACTCCTGT GTGGAACTGG CCCTCTCTTC CCGTAGAGCA GTTTGTTCTC
    ACGACAGAAG CGGATTCTCC CTCATCTGTT ACCTGGCAAG GAATTACAAA TCTCAAATTA
    GTCACCCTGA CAGCAACAGC GAAGGAGAAG ATGAGAAGCC TCATCATTTA CTCTCTGCCT
    TCGATGGAAA CACTGTACTC TTTGGAAGTA TCTAGTGTTT CTTCTTTAGT TCAAACAGGA
    ATCAGCACGG ACACTATATA TCTTCTGGAA GGAATTCACA AAAATGATCC TAACCTATGT
    GAAGACTCTG TGTCTGACTT AGTACTTAGG TACCTCACAG AGGTGTTACC AGAAAACAGA
    CTGAGCCGGT TACTTCACAA GCACAGATTT GCCGAAGCCG AGAGCTTTGC CATTCAGTTC
    GGACTGGATG TTGAGCTTGT TTATAAGGTC AAATCAAATG ATATGTTGGA GAAGCTGGCT
    TTGATTTCCT CGGACAAGAG TGAGCAGTCC AAATGGCAGC AACTTGTAGA TGAAGCTAAA
    GAAAACCTAT GCAAAATCCA GGACGATGAC TTCGTGGTGA ATTTCTGCCT CAAGGCCCAA
    TGGGTAACCT ATGAAACCAC CCAGGAAATG CTCAGTTATG CCAAAACCAG GCTCATGAAG
    AAAGAAGACA GAGCTCTACC TGCCTCTTCT GATGCCTTCA TGGAGGTGCT AAAAGCTCAT
    GCAAAGTTAA CTACATTTTA TGGAGCATTT GGACCAGAAA AATTCAGTGG TAGCTCTTGG
    ATTGAGTTCC TGAATAACGA AGATGACCTC AGGGATGTTT TTTTGCAGCT AAGTGAAGGA
    AACTTCGCTT GTGCTCAGTA TCTTTGGCTT AGACATCGGG CTGACTTTGA AAGCAAGTTT
    GACGTGAAAA TGCTGGAGAA TTTGCTGAAT TCAATATCTA CACAATTCCC CTTGGAAAAC
    CTTTGTTCCT GGTTTAAAAA TGAAGTGATT CCGTTTGTGA GGAGGATTGT GCCCGAAGGA
    CAAAACATTC TTGCAAAATG GCTAGAACAA GCATCAAGGA ACCTAGAGTT AACTGATAAG
    GCAAACTGGC CAGAAAATGG ACTTCAGCTG GCAGAGGTGT TCTTCACTGC AGAAAAAACA
    GACCGGTTTG GATTCGCATC TTCTTGGCAC TGGATTTCCT TGGATTATCA AAACACAGAG
    GAAGTCCGCC AGTTACGGAC ACTGGTGAGC AAGTTGCGGG AGCTGATCAT TCTGCATAGG
    AAATACAACT GCAAGCTGGC CCTCTCGGAC TTTGAGAAGG AAAATGCCAC CACTGTGGTG
    TTCCGCATGT TCGACAGGGT CTCGGCCCCA GAGCTGATCC CTTCGGTCTT AGAGAAGTCT
    GTGAGAGTTT ACATTCGCGA ACAGAATCTG CAAGAGGAGG AGCTGCTGTT GCTCTATATA
    GAGGATTTAC TGAAGAGGTG TAGCTCAAAG TCAATGACTC TCTTCGACAC AGCGTGGGAA
    GCAAAGGCCA TGGCCGTGAT AAGATGCTTG TCTGACACAG ATCTCATCTT TGATGCTGTG
    CTGAAGATAA TGTACAAGGC GGTGGTTCCC TGGAGTGCAG CCGTGGAGCA GCTGGTGAAA
    CAGCACCTGG AAATGGACCA CCCCAAAGTC AAGTTATTGC AGGAAAGCTA CAAACTGATG
    GAGATGAAGA AACTTTTGCG AGGGTATGGG ATAAGAGAGG TGAATCTCCT GAACAAGGAA
    ATAATGAGAG TGATCCGATA CATTCTAAAG CAGGACATCC CGTCTTCTTT AGAAGATGCT
    CTGAAGGTGG CCCAGGGGTA CAGGCTGTCT GATGACGAAA TCTACAGCCT GAGAATCATC
    GATCTGATTG ACAGGGAGCA GGGGGGAGAT TGCCTCCTGC TACTGAAGTC TCTGCCTGCC
    GCTGAAGCCG AGAAGACTGC AGAAAGGGTT ATCATCTGGG CTCGACTGGC ACTGCAGGAG
    GAGCCTGACG GCTCTGAGGA GGACAAGGCC TGGAGAATAT CTGTGGCGAA GACATCGGTG
    GATATTCTCA AGATCCTGTG TGATATTCGA AAAGACAATC TGCAGAAGAA GGATGAATCT
    GAAGAATTCC TGAAGCGATT TCAAATGGTT GCTAGTTTAC AGGAGAACTT TGAGGTCTTC
    CTTCCATTTG AAGACTACAG CAACACCGCC TTGGTAGCTG GCCTTCGAGA ACAGTATATT
    AAGGCACAGG AAGCTGCACA GGCTGAGCAC AAACACAGGG GACCCCCCGG GCCCACCCCG
    GCTAGGGGGA CACACCTGAG CATCAAGTCA AAGCTGCACC GACAGGCACT GGCCCTGCAG
    GTGTCTGAAC AGGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAGGA CGGGAAGGTG
    GTGGCCGCCC TGAGCAAATG CAGAGATTTA CTTAAGCATT ACTGCAATGC CGACACTGGG
    AGGTTACTGT TTGTGGTGTG TCAGAAGCTC TGTCAGATGT TGGCTGACGA TGTCCCAATG
    GTGGCCCCAG GAGGACTCAG CCTTCCTTCT GAGATCCATG ACCTCGCATG CCACGCTGTC
    ACCATCTGCA GTCCAGATTA TTTGCTGGAT GTTTTAGAAC TAAGTAAATA CACTTTAACC
    GCTGTGGAGC TTTGCAGACA ATGCCAGATG GATGACTGTG GAATGCTGAT GAAGGCCGCT
    CTTGGAACTC ACAAGGATCC CTATGAAGAG TGGTCCTTTA GTGACTTCTT CAGTGAAGAT
    GGAATTGTTC TCGAGTCCCA GGTGGTGCTG CCTGTCATCT ATGAATTGAT CTCATCAGTG
    ATGCCCCCTG CAGAAAGCAA AAGACATCCT TTGGATTCTA TAAGTTTGCC ATACTGCTCC
    ACTAGTGAAG GAGAAAACCG TATTCTCCCT CTTGTAAGCT CCATCTCTGC TCTGCTCCGG
    AGCCTCCAGG AATGTAGTCA GTGGGAGCTA GCCCTGAGGT TCGTGGTGGG CTCCTTTGGT
    ACCTGCCTGC AGCACTCTAT GTCAAACGTC ATGAGTATCA GCTTGAGTAA ACAGAGATCC
    ACCTGCCTCT GTCTCCCGAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35849
    Clone ID Related Accession (Same CDS sequence) XM_006530254.2
    Accession Version XM_006530254.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product kinetochore-associated protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006530254.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGGGGAG ATTGCCTCCT GCTACTGAAG TCTCTGCCTG CCGCTGAAGC CGAGAAGACT 
    GCAGAAAGGG TTATCATCTG GGCTCGACTG GCACTGCAGG AGGAGCCTGA CGGCTCTGAG
    GAGGACAAGG CCTGGAGAAT ATCTGTGGCG AAGACATCGG TGGATATTCT CAAGATCCTG
    TGTGATATTC GAAAAGACAA TCTGCAGAAG AAGGATGAAT CTGAAGAATT CCTGAAGCGA
    TTTCAAATGG TTGCTAGTTT ACAGGAGAAC TTTGAGGTCT TCCTTCCATT TGAAGACTAC
    AGCAACACCG CCTTGGTAGC TGGCCTTCGA GAACAGTATA TTAAGGCACA GGAAGCTGCA
    CAGGCTGAGC ACAAACACAG GGGACCCCCC GGGCCCACCC CGGCTAGGGG GACACACCTG
    AGCATCAAGT CAAAGCTGCA CCGACAGGCA CTGGCCCTGC AGGTGTCTGA ACAGGAGCTG
    GAGGCAGAGC TGACCTTGAG AGCCTTAAAG GACGGGAAGG TGGTGGCCGC CCTGAGCAAA
    TGCAGAGATT TACTTAAGCA TTACTGCAAT GCCGACACTG GGAGGTTACT GTTTGTGGTG
    TGTCAGAAGC TCTGTCAGAT GTTGGCTGAC GATGTCCCAA TGGTGGCCCC AGGAGGACTC
    AGCCTTCCTT CTGAGATCCA TGACCTCGCA TGCCACGCTG TCACCATCTG CAGTCCAGAT
    TATTTGCTGG ATGTTTTAGA ACTAAGTAAA TACACTTTAA CCGCTGTGGA GCTTTGCAGA
    CAATGCCAGA TGGATGACTG TGGAATGCTG ATGAAGGCCG CTCTTGGAAC TCACAAGGAT
    CCCTATGAAG AGTGGTCCTT TAGTGACTTC TTCAGTGAAG ATGGAATTGT TCTCGAGTCC
    CAGGTGGTGC TGCCTGTCAT CTATGAATTG ATCTCATCAG TGATGCCCCC TGCAGAAAGC
    AAAAGACATC CTTTGGATTC TATAAGTTTG CCATACTGCT CCACTAGTGA AGGAGAAAAC
    CGTATTCTCC CTCTTGTAAG CTCCATCTCT GCTCTGCTCC GGAGCCTCCA GGAATGTAGT
    CAGTGGGAGC TAGCCCTGAG GTTCGTGGTG GGCTCCTTTG GTACCTGCCT GCAGCACTCT
    ATGTCAAACG TCATGAGTAT CAGCTTGAGT AAACAGTTAC TTGGGAAGAA CACATTGGCG
    AACTCCAGGC ATATCATTAT GGAATTGAAA GAGAAGTCCA TCACATTTAT CAGAGAAAAT
    GCTACGACAC TCCTGCATAA AGTGTTTAAC TGTCGGGTGG TGGATCTGGA CCTCGCCTTG
    GCTTACTGCA CTCTCTTACC TCAAAAGGAT GTATTCGACA ACCTCTGGAA GTTCATAGAT
    AAAGCGTGGC AGAATTACGA CAAAATTCTG GCATTATCTT TGGTGGGCTC TCAGCTGGCA
    AATCTCTACC AGGATATTGA AACAGGACTT TGGTTCCACG AGCTCAGTAT CGATGCCAAG
    TGGGGTATTC GTCTTGGTAA ACTTGGTATT TCTTTTCAGC CTGCTTTTAG ACAAAATTTT
    CTCACCAAGA AAGACCTTAT CAAAGCTCTT GTGAATAACA TAGACATGGA CACCAGCCTC
    ATTCTGGAGT ATTGCAGCAC ATTTCAGCTG GACTCTGACG CTGCCCTTCG GCTGTTCATT
    GAAACTCTGC TCCGCAACAC TAGCAGCCAG AGCCAGGGAG ATGCTGCCCC GGAGTCCACA
    AAGCATCAGC ATTCCAAACT CCTGGCGAAA GCCACTGAGC TGGTCCCTTT ACTGAAGAAC
    ACAAAGGATC TGGTGATCAG TCTTAGTGAG ATCCTCTATA AGCTGGACCC ATATGATTAT
    GAAATGATTG ATGTTGTCCT GAAAGTTTTA GAACAAGCTA ACGAAAAGAT AACCAGTGTG
    AACATCAATC AGGCGCTGAA TCTTCTGAGA CACTTGAAGT CATACAGAAG GATCTCCCCG
    CCAGTGGACC ACGAATACCA GTACGCGCTG GAGCACATGA TCACTCTGCC ACCGGCTGCT
    CACACTAGAC TGCCCTTTCA CCTAATACTG TTTGGCACAG CACAGAACTT CTGGAAAATT
    CTCTCCTCAG AGCTCAGCGA AGAGTCGCTG CCAACCCTGC TCTTAATCGC GAAGCTGATG
    AAGTTCTCCC TAGATACTCT CTATGTGTCT ACCGCCAAAC ACTTGTTTGA AAAAAACCTG
    AAGCCAAAGC TCCTGAAGTC GGCACAAGCC AGGTCCTCCA CCCTGATGAG CAAGGAAGTG
    GATAAGCTCA TGCAGACGCT GGAGTCGTAC CTCCTGTCCA TCGTCAACCC CGAGTGGGCT
    GTCGCCATTG CCATCAGCCT CACCCAGGAA GTCCCAGAAG GCCCCTTCAA GATGTCTAGT
    TTGAAGTTCT GCCTGTATTT AGCTGAGAGA TGGCTACAAA ACATCCCACC TCAGGATGAG
    ACGTGTGAAA AGGCCAAAGC CTTGCAGAAG AAGCTCTGCC TCCAGGTCCG GCTCTCAGGA
    ACGGAAGCCG TGCTCATAGC CCACAAGCTG AACGATCAGG AATATTTAAG AGTGATAGGG
    AAGCCAGCGC ACCTCATCGT GAGCCTCTAC GAACATCCCA GCATAAGCGA AAGGCTTTGT
    ACCACATCTG GCAAAGATTA TCCCGATATC CATACAGCCG CTAAAGAAAT AGCCGAAGTC
    AATGAAGTTA ATTTGGAAAA AATCTGGGAC ATGTTGTTGG AGAAATGGCT GTGCCCCTCA
    ACGGTGCCCA GTGAGAAAGC ATCGGAGTTC TTTGAGCTGG AAGAGGATGA AGTGCTGCAT
    AGAGTCGTGT ATCTCCTCCA GGCTCGTCCT GTTGATTATT GTTCGAGAAT GCTGTTTGTA
    TTTGCCACTT CAGCTACAAG TACACTGGGC ATGCGCCAGC TAACGTTTGC CCACAAAGCA
    CGAGCTCTGC AGTGTCTCCT CTATTTGGCT GACAAGGAAA CAATTGAATC TCTCTTCAAA
    AAGCCCATTA AAGAAATGAA ATCCTATCTG AAATGTATAA CTTTCCTGGC ATCATTTGAG
    ACATTGAATA TTCCCATCAC ATATGAATTA TTCTGCAACA GTCCCAAAGA AGGCATGATT
    AAGGGCTTGT GGAAGAACCA CAGCCACGAA CCTATGGCAG TACGATTGGT TGCAGAGCTT
    TGCTTAGAAT ACAAAATCTA TGACCTGCAG CTTTGGAATG GGCTCTTGCA GAAGCTTCTG
    GGCTTCAACA TGATTCCTTA CCTGAGGAAA GTGTTATCCT GCATTTCTAG CATCCATTCG
    TTATGGCAGG TGCCCTACTT CAGCAAAGCG TGGCAGCGCG TGATCCAGAT CCCTCTGCTC
    TCGGCTTCCT GTCCTTTAAG ACCCAGTCAG TTAGCAGACT GCTGTGACAG TCTCGTCGCC
    ATCCTTGAAT GTCCAGTTTC AGATGACCTG GACATGATGG GAGTCGCCAA GCAGTACGTC
    CAGTTAGACC TTCCCGCTTT TGCTCTGACC TGCCTGACGC TCATGCCCCA CTCTGAAAAA
    AGACACCAGC AGATCAAGAA TTTTCTGAAT TCCTGTGATG CTCGGATCAT TTTACAGCAG
    ATAGAAGAAC ATATGAACAC TGGCCAGCTA GCAGGGTTTT CACATCAGAT TGGAAGTCTG
    GTTCTGAATC ATGTTGTAAA TAAGAAGGAA TTTGGGATTT TGGCAAAGAC AAAGTATTTT
    CAGTTGTTGA AATGCCACGT GATAAACACC GGCAACGTCA CTGAATTGGT AAACTATTTG
    GCAAATGACT TCAGTGTAGA TGAAGCATCA GCGTTGATAA ATGAGTATTC AAAGCATTGT
    GGGAAGCCTG TGCCAGCAGA TGCAGCTCCC TGTGAGATCC TGCAGACATT TCTTGGTGGA
    TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006530317.1
    CDS86..3991
    Translation

    Target ORF information:

    RefSeq Version XM_006530254.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus kinetochore associated 1 (Kntc1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006530254.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGGGGAG ATTGCCTCCT GCTACTGAAG TCTCTGCCTG CCGCTGAAGC CGAGAAGACT 
    GCAGAAAGGG TTATCATCTG GGCTCGACTG GCACTGCAGG AGGAGCCTGA CGGCTCTGAG
    GAGGACAAGG CCTGGAGAAT ATCTGTGGCG AAGACATCGG TGGATATTCT CAAGATCCTG
    TGTGATATTC GAAAAGACAA TCTGCAGAAG AAGGATGAAT CTGAAGAATT CCTGAAGCGA
    TTTCAAATGG TTGCTAGTTT ACAGGAGAAC TTTGAGGTCT TCCTTCCATT TGAAGACTAC
    AGCAACACCG CCTTGGTAGC TGGCCTTCGA GAACAGTATA TTAAGGCACA GGAAGCTGCA
    CAGGCTGAGC ACAAACACAG GGGACCCCCC GGGCCCACCC CGGCTAGGGG GACACACCTG
    AGCATCAAGT CAAAGCTGCA CCGACAGGCA CTGGCCCTGC AGGTGTCTGA ACAGGAGCTG
    GAGGCAGAGC TGACCTTGAG AGCCTTAAAG GACGGGAAGG TGGTGGCCGC CCTGAGCAAA
    TGCAGAGATT TACTTAAGCA TTACTGCAAT GCCGACACTG GGAGGTTACT GTTTGTGGTG
    TGTCAGAAGC TCTGTCAGAT GTTGGCTGAC GATGTCCCAA TGGTGGCCCC AGGAGGACTC
    AGCCTTCCTT CTGAGATCCA TGACCTCGCA TGCCACGCTG TCACCATCTG CAGTCCAGAT
    TATTTGCTGG ATGTTTTAGA ACTAAGTAAA TACACTTTAA CCGCTGTGGA GCTTTGCAGA
    CAATGCCAGA TGGATGACTG TGGAATGCTG ATGAAGGCCG CTCTTGGAAC TCACAAGGAT
    CCCTATGAAG AGTGGTCCTT TAGTGACTTC TTCAGTGAAG ATGGAATTGT TCTCGAGTCC
    CAGGTGGTGC TGCCTGTCAT CTATGAATTG ATCTCATCAG TGATGCCCCC TGCAGAAAGC
    AAAAGACATC CTTTGGATTC TATAAGTTTG CCATACTGCT CCACTAGTGA AGGAGAAAAC
    CGTATTCTCC CTCTTGTAAG CTCCATCTCT GCTCTGCTCC GGAGCCTCCA GGAATGTAGT
    CAGTGGGAGC TAGCCCTGAG GTTCGTGGTG GGCTCCTTTG GTACCTGCCT GCAGCACTCT
    ATGTCAAACG TCATGAGTAT CAGCTTGAGT AAACAGTTAC TTGGGAAGAA CACATTGGCG
    AACTCCAGGC ATATCATTAT GGAATTGAAA GAGAAGTCCA TCACATTTAT CAGAGAAAAT
    GCTACGACAC TCCTGCATAA AGTGTTTAAC TGTCGGGTGG TGGATCTGGA CCTCGCCTTG
    GCTTACTGCA CTCTCTTACC TCAAAAGGAT GTATTCGACA ACCTCTGGAA GTTCATAGAT
    AAAGCGTGGC AGAATTACGA CAAAATTCTG GCATTATCTT TGGTGGGCTC TCAGCTGGCA
    AATCTCTACC AGGATATTGA AACAGGACTT TGGTTCCACG AGCTCAGTAT CGATGCCAAG
    TGGGGTATTC GTCTTGGTAA ACTTGGTATT TCTTTTCAGC CTGCTTTTAG ACAAAATTTT
    CTCACCAAGA AAGACCTTAT CAAAGCTCTT GTGAATAACA TAGACATGGA CACCAGCCTC
    ATTCTGGAGT ATTGCAGCAC ATTTCAGCTG GACTCTGACG CTGCCCTTCG GCTGTTCATT
    GAAACTCTGC TCCGCAACAC TAGCAGCCAG AGCCAGGGAG ATGCTGCCCC GGAGTCCACA
    AAGCATCAGC ATTCCAAACT CCTGGCGAAA GCCACTGAGC TGGTCCCTTT ACTGAAGAAC
    ACAAAGGATC TGGTGATCAG TCTTAGTGAG ATCCTCTATA AGCTGGACCC ATATGATTAT
    GAAATGATTG ATGTTGTCCT GAAAGTTTTA GAACAAGCTA ACGAAAAGAT AACCAGTGTG
    AACATCAATC AGGCGCTGAA TCTTCTGAGA CACTTGAAGT CATACAGAAG GATCTCCCCG
    CCAGTGGACC ACGAATACCA GTACGCGCTG GAGCACATGA TCACTCTGCC ACCGGCTGCT
    CACACTAGAC TGCCCTTTCA CCTAATACTG TTTGGCACAG CACAGAACTT CTGGAAAATT
    CTCTCCTCAG AGCTCAGCGA AGAGTCGCTG CCAACCCTGC TCTTAATCGC GAAGCTGATG
    AAGTTCTCCC TAGATACTCT CTATGTGTCT ACCGCCAAAC ACTTGTTTGA AAAAAACCTG
    AAGCCAAAGC TCCTGAAGTC GGCACAAGCC AGGTCCTCCA CCCTGATGAG CAAGGAAGTG
    GATAAGCTCA TGCAGACGCT GGAGTCGTAC CTCCTGTCCA TCGTCAACCC CGAGTGGGCT
    GTCGCCATTG CCATCAGCCT CACCCAGGAA GTCCCAGAAG GCCCCTTCAA GATGTCTAGT
    TTGAAGTTCT GCCTGTATTT AGCTGAGAGA TGGCTACAAA ACATCCCACC TCAGGATGAG
    ACGTGTGAAA AGGCCAAAGC CTTGCAGAAG AAGCTCTGCC TCCAGGTCCG GCTCTCAGGA
    ACGGAAGCCG TGCTCATAGC CCACAAGCTG AACGATCAGG AATATTTAAG AGTGATAGGG
    AAGCCAGCGC ACCTCATCGT GAGCCTCTAC GAACATCCCA GCATAAGCGA AAGGCTTTGT
    ACCACATCTG GCAAAGATTA TCCCGATATC CATACAGCCG CTAAAGAAAT AGCCGAAGTC
    AATGAAGTTA ATTTGGAAAA AATCTGGGAC ATGTTGTTGG AGAAATGGCT GTGCCCCTCA
    ACGGTGCCCA GTGAGAAAGC ATCGGAGTTC TTTGAGCTGG AAGAGGATGA AGTGCTGCAT
    AGAGTCGTGT ATCTCCTCCA GGCTCGTCCT GTTGATTATT GTTCGAGAAT GCTGTTTGTA
    TTTGCCACTT CAGCTACAAG TACACTGGGC ATGCGCCAGC TAACGTTTGC CCACAAAGCA
    CGAGCTCTGC AGTGTCTCCT CTATTTGGCT GACAAGGAAA CAATTGAATC TCTCTTCAAA
    AAGCCCATTA AAGAAATGAA ATCCTATCTG AAATGTATAA CTTTCCTGGC ATCATTTGAG
    ACATTGAATA TTCCCATCAC ATATGAATTA TTCTGCAACA GTCCCAAAGA AGGCATGATT
    AAGGGCTTGT GGAAGAACCA CAGCCACGAA CCTATGGCAG TACGATTGGT TGCAGAGCTT
    TGCTTAGAAT ACAAAATCTA TGACCTGCAG CTTTGGAATG GGCTCTTGCA GAAGCTTCTG
    GGCTTCAACA TGATTCCTTA CCTGAGGAAA GTGTTATCCT GCATTTCTAG CATCCATTCG
    TTATGGCAGG TGCCCTACTT CAGCAAAGCG TGGCAGCGCG TGATCCAGAT CCCTCTGCTC
    TCGGCTTCCT GTCCTTTAAG ACCCAGTCAG TTAGCAGACT GCTGTGACAG TCTCGTCGCC
    ATCCTTGAAT GTCCAGTTTC AGATGACCTG GACATGATGG GAGTCGCCAA GCAGTACGTC
    CAGTTAGACC TTCCCGCTTT TGCTCTGACC TGCCTGACGC TCATGCCCCA CTCTGAAAAA
    AGACACCAGC AGATCAAGAA TTTTCTGAAT TCCTGTGATG CTCGGATCAT TTTACAGCAG
    ATAGAAGAAC ATATGAACAC TGGCCAGCTA GCAGGGTTTT CACATCAGAT TGGAAGTCTG
    GTTCTGAATC ATGTTGTAAA TAAGAAGGAA TTTGGGATTT TGGCAAAGAC AAAGTATTTT
    CAGTTGTTGA AATGCCACGT GATAAACACC GGCAACGTCA CTGAATTGGT AAACTATTTG
    GCAAATGACT TCAGTGTAGA TGAAGCATCA GCGTTGATAA ATGAGTATTC AAAGCATTGT
    GGGAAGCCTG TGCCAGCAGA TGCAGCTCCC TGTGAGATCC TGCAGACATT TCTTGGTGGA
    TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35846
    Clone ID Related Accession (Same CDS sequence) XM_006530251.4 , XM_006530251.3
    Accession Version XM_006530251.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product kinetochore-associated protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530251.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTACAAGG CGGTGGTTCC CTGGAGTGCA GCCGTGGAGC AGCTGGTGAA ACAGCACCTG 
    GAAATGGACC ACCCCAAAGT CAAGTTATTG CAGGAAAGCT ACAAACTGAT GGAGATGAAG
    AAACTTTTGC GAGGGTATGG GATAAGAGAG GTGAATCTCC TGAACAAGGA AATAATGAGA
    GTGATCCGAT ACATTCTAAA GCAGGACATC CCGTCTTCTT TAGAAGATGC TCTGAAGGTG
    GCCCAGGGGT ACAGGCTGTC TGATGACGAA ATCTACAGCC TGAGAATCAT CGATCTGATT
    GACAGGGAGC AGGGGGGAGA TTGCCTCCTG CTACTGAAGT CTCTGCCTGC CGCTGAAGCC
    GAGAAGACTG CAGAAAGGGT TATCATCTGG GCTCGACTGG CACTGCAGGA GGAGCCTGAC
    GGCTCTGAGG AGGACAAGGC CTGGAGAATA TCTGTGGCGA AGACATCGGT GGATATTCTC
    AAGATCCTGT GTGATATTCG AAAAGACAAT CTGCAGAAGA AGGATGAATC TGAAGAATTC
    CTGAAGCGAT TTCAAATGGT TGCTAGTTTA CAGGAGAACT TTGAGGTCTT CCTTCCATTT
    GAAGACTACA GCAACACCGC CTTGGTAGCT GGCCTTCGAG AACAGTATAT TAAGGCACAG
    GAAGCTGCAC AGGCTGAGCA CAAACACAGG GGACCCCCCG GGCCCACCCC GGCTAGGGGG
    ACACACCTGA GCATCAAGTC AAAGCTGCAC CGACAGGCAC TGGCCCTGCA GGTGTCTGAA
    CAGGAGCTGG AGGCAGAGCT GACCTTGAGA GCCTTAAAGG ACGGGAAGGT GGTGGCCGCC
    CTGAGCAAAT GCAGAGATTT ACTTAAGCAT TACTGCAATG CCGACACTGG GAGGTTACTG
    TTTGTGGTGT GTCAGAAGCT CTGTCAGATG TTGGCTGACG ATGTCCCAAT GGTGGCCCCA
    GGAGGACTCA GCCTTCCTTC TGAGATCCAT GACCTCGCAT GCCACGCTGT CACCATCTGC
    AGTCCAGATT ATTTGCTGGA TGTTTTAGAA CTAAGTAAAT ACACTTTAAC CGCTGTGGAG
    CTTTGCAGAC AATGCCAGAT GGATGACTGT GGAATGCTGA TGAAGGCCGC TCTTGGAACT
    CACAAGGATC CCTATGAAGA GTGGTCCTTT AGTGACTTCT TCAGTGAAGA TGGAATTGTT
    CTCGAGTCCC AGGTGGTGCT GCCTGTCATC TATGAATTGA TCTCATCAGT GATGCCCCCT
    GCAGAAAGCA AAAGACATCC TTTGGATTCT ATAAGTTTGC CATACTGCTC CACTAGTGAA
    GGAGAAAACC GTATTCTCCC TCTTGTAAGC TCCATCTCTG CTCTGCTCCG GAGCCTCCAG
    GAATGTAGTC AGTGGGAGCT AGCCCTGAGG TTCGTGGTGG GCTCCTTTGG TACCTGCCTG
    CAGCACTCTA TGTCAAACGT CATGAGTATC AGCTTGAGTA AACAGTTACT TGGGAAGAAC
    ACATTGGCGA ACTCCAGGCA TATCATTATG GAATTGAAAG AGAAGTCCAT CACATTTATC
    AGAGAAAATG CTACGACACT CCTGCATAAA GTGTTTAACT GTCGGGTGGT GGATCTGGAC
    CTCGCCTTGG CTTACTGCAC TCTCTTACCT CAAAAGGATG TATTCGACAA CCTCTGGAAG
    TTCATAGATA AAGCGTGGCA GAATTACGAC AAAATTCTGG CATTATCTTT GGTGGGCTCT
    CAGCTGGCAA ATCTCTACCA GGATATTGAA ACAGGACTTT GGTTCCACGA GCTCAGTATC
    GATGCCAAGT GGGGTATTCG TCTTGGTAAA CTTGGTATTT CTTTTCAGCC TGCTTTTAGA
    CAAAATTTTC TCACCAAGAA AGACCTTATC AAAGCTCTTG TGAATAACAT AGACATGGAC
    ACCAGCCTCA TTCTGGAGTA TTGCAGCACA TTTCAGCTGG ACTCTGACGC TGCCCTTCGG
    CTGTTCATTG AAACTCTGCT CCGCAACACT AGCAGCCAGA GCCAGGGAGA TGCTGCCCCG
    GAGTCCACAA AGCATCAGCA TTCCAAACTC CTGGCGAAAG CCACTGAGCT GGTCCCTTTA
    CTGAAGAACA CAAAGGATCT GGTGATCAGT CTTAGTGAGA TCCTCTATAA GCTGGACCCA
    TATGATTATG AAATGATTGA TGTTGTCCTG AAAGTTTTAG AACAAGCTAA CGAAAAGATA
    ACCAGTGTGA ACATCAATCA GGCGCTGAAT CTTCTGAGAC ACTTGAAGTC ATACAGAAGG
    ATCTCCCCGC CAGTGGACCA CGAATACCAG TACGCGCTGG AGCACATGAT CACTCTGCCA
    CCGGCTGCTC ACACTAGACT GCCCTTTCAC CTAATACTGT TTGGCACAGC ACAGAACTTC
    TGGAAAATTC TCTCCTCAGA GCTCAGCGAA GAGTCGCTGC CAACCCTGCT CTTAATCGCG
    AAGCTGATGA AGTTCTCCCT AGATACTCTC TATGTGTCTA CCGCCAAACA CTTGTTTGAA
    AAAAACCTGA AGCCAAAGCT CCTGAAGTCG GCACAAGCCA GGTCCTCCAC CCTGATGAGC
    AAGGAAGTGG ATAAGCTCAT GCAGACGCTG GAGTCGTACC TCCTGTCCAT CGTCAACCCC
    GAGTGGGCTG TCGCCATTGC CATCAGCCTC ACCCAGGAAG TCCCAGAAGG CCCCTTCAAG
    ATGTCTAGTT TGAAGTTCTG CCTGTATTTA GCTGAGAGAT GGCTACAAAA CATCCCACCT
    CAGGATGAGA CGTGTGAAAA GGCCAAAGCC TTGCAGAAGA AGCTCTGCCT CCAGGTCCGG
    CTCTCAGGAA CGGAAGCCGT GCTCATAGCC CACAAGCTGA ACGATCAGGA ATATTTAAGA
    GTGATAGGGA AGCCAGCGCA CCTCATCGTG AGCCTCTACG AACATCCCAG CATAAGCGAA
    AGGCTTTGTA CCACATCTGG CAAAGATTAT CCCGATATCC ATACAGCCGC TAAAGAAATA
    GCCGAAGTCA ATGAAGTTAA TTTGGAAAAA ATCTGGGACA TGTTGTTGGA GAAATGGCTG
    TGCCCCTCAA CGGTGCCCAG TGAGAAAGCA TCGGAGTTCT TTGAGCTGGA AGAGGATGAA
    GTGCTGCATA GAGTCGTGTA TCTCCTCCAG GCTCGTCCTG TTGATTATTG TTCGAGAATG
    CTGTTTGTAT TTGCCACTTC AGCTACAAGT ACACTGGGCA TGCGCCAGCT AACGTTTGCC
    CACAAAGCAC GAGCTCTGCA GTGTCTCCTC TATTTGGCTG ACAAGGAAAC AATTGAATCT
    CTCTTCAAAA AGCCCATTAA AGAAATGAAA TCCTATCTGA AATGTATAAC TTTCCTGGCA
    TCATTTGAGA CATTGAATAT TCCCATCACA TATGAATTAT TCTGCAACAG TCCCAAAGAA
    GGCATGATTA AGGGCTTGTG GAAGAACCAC AGCCACGAAC CTATGGCAGT ACGATTGGTT
    GCAGAGCTTT GCTTAGAATA CAAAATCTAT GACCTGCAGC TTTGGAATGG GCTCTTGCAG
    AAGCTTCTGG GCTTCAACAT GATTCCTTAC CTGAGGAAAG TGTTATCCTG CATTTCTAGC
    ATCCATTCGT TATGGCAGGT GCCCTACTTC AGCAAAGCGT GGCAGCGCGT GATCCAGATC
    CCTCTGCTCT CGGCTTCCTG TCCTTTAAGA CCCAGTCAGT TAGCAGACTG CTGTGACAGT
    CTCGTCGCCA TCCTTGAATG TCCAGTTTCA GATGACCTGG ACATGATGGG AGTCGCCAAG
    CAGTACGTCC AGTTAGACCT TCCCGCTTTT GCTCTGACCT GCCTGACGCT CATGCCCCAC
    TCTGAAAAAA GACACCAGCA GATCAAGAAT TTTCTGAATT CCTGTGATGC TCGGATCATT
    TTACAGCAGA TAGAAGAACA TATGAACACT GGCCAGCTAG CAGGGTTTTC ACATCAGATT
    GGAAGTCTGG TTCTGAATCA TGTTGTAAAT AAGAAGGAAT TTGGGATTTT GGCAAAGACA
    AAGTATTTTC AGTTGTTGAA ATGCCACGTG ATAAACACCG GCAACGTCAC TGAATTGGTA
    AACTATTTGG CAAATGACTT CAGTGTAGAT GAAGCATCAG CGTTGATAAA TGAGTATTCA
    AAGCATTGTG GGAAGCCTGT GCCAGCAGAT GCAGCTCCCT GTGAGATCCT GCAGACATTT
    CTTGGTGGAT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006530314.1
    CDS104..4318
    Translation

    Target ORF information:

    RefSeq Version XM_006530251.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus kinetochore associated 1 (Kntc1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006530251.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTACAAGG CGGTGGTTCC CTGGAGTGCA GCCGTGGAGC AGCTGGTGAA ACAGCACCTG 
    GAAATGGACC ACCCCAAAGT CAAGTTATTG CAGGAAAGCT ACAAACTGAT GGAGATGAAG
    AAACTTTTGC GAGGGTATGG GATAAGAGAG GTGAATCTCC TGAACAAGGA AATAATGAGA
    GTGATCCGAT ACATTCTAAA GCAGGACATC CCGTCTTCTT TAGAAGATGC TCTGAAGGTG
    GCCCAGGGGT ACAGGCTGTC TGATGACGAA ATCTACAGCC TGAGAATCAT CGATCTGATT
    GACAGGGAGC AGGGGGGAGA TTGCCTCCTG CTACTGAAGT CTCTGCCTGC CGCTGAAGCC
    GAGAAGACTG CAGAAAGGGT TATCATCTGG GCTCGACTGG CACTGCAGGA GGAGCCTGAC
    GGCTCTGAGG AGGACAAGGC CTGGAGAATA TCTGTGGCGA AGACATCGGT GGATATTCTC
    AAGATCCTGT GTGATATTCG AAAAGACAAT CTGCAGAAGA AGGATGAATC TGAAGAATTC
    CTGAAGCGAT TTCAAATGGT TGCTAGTTTA CAGGAGAACT TTGAGGTCTT CCTTCCATTT
    GAAGACTACA GCAACACCGC CTTGGTAGCT GGCCTTCGAG AACAGTATAT TAAGGCACAG
    GAAGCTGCAC AGGCTGAGCA CAAACACAGG GGACCCCCCG GGCCCACCCC GGCTAGGGGG
    ACACACCTGA GCATCAAGTC AAAGCTGCAC CGACAGGCAC TGGCCCTGCA GGTGTCTGAA
    CAGGAGCTGG AGGCAGAGCT GACCTTGAGA GCCTTAAAGG ACGGGAAGGT GGTGGCCGCC
    CTGAGCAAAT GCAGAGATTT ACTTAAGCAT TACTGCAATG CCGACACTGG GAGGTTACTG
    TTTGTGGTGT GTCAGAAGCT CTGTCAGATG TTGGCTGACG ATGTCCCAAT GGTGGCCCCA
    GGAGGACTCA GCCTTCCTTC TGAGATCCAT GACCTCGCAT GCCACGCTGT CACCATCTGC
    AGTCCAGATT ATTTGCTGGA TGTTTTAGAA CTAAGTAAAT ACACTTTAAC CGCTGTGGAG
    CTTTGCAGAC AATGCCAGAT GGATGACTGT GGAATGCTGA TGAAGGCCGC TCTTGGAACT
    CACAAGGATC CCTATGAAGA GTGGTCCTTT AGTGACTTCT TCAGTGAAGA TGGAATTGTT
    CTCGAGTCCC AGGTGGTGCT GCCTGTCATC TATGAATTGA TCTCATCAGT GATGCCCCCT
    GCAGAAAGCA AAAGACATCC TTTGGATTCT ATAAGTTTGC CATACTGCTC CACTAGTGAA
    GGAGAAAACC GTATTCTCCC TCTTGTAAGC TCCATCTCTG CTCTGCTCCG GAGCCTCCAG
    GAATGTAGTC AGTGGGAGCT AGCCCTGAGG TTCGTGGTGG GCTCCTTTGG TACCTGCCTG
    CAGCACTCTA TGTCAAACGT CATGAGTATC AGCTTGAGTA AACAGTTACT TGGGAAGAAC
    ACATTGGCGA ACTCCAGGCA TATCATTATG GAATTGAAAG AGAAGTCCAT CACATTTATC
    AGAGAAAATG CTACGACACT CCTGCATAAA GTGTTTAACT GTCGGGTGGT GGATCTGGAC
    CTCGCCTTGG CTTACTGCAC TCTCTTACCT CAAAAGGATG TATTCGACAA CCTCTGGAAG
    TTCATAGATA AAGCGTGGCA GAATTACGAC AAAATTCTGG CATTATCTTT GGTGGGCTCT
    CAGCTGGCAA ATCTCTACCA GGATATTGAA ACAGGACTTT GGTTCCACGA GCTCAGTATC
    GATGCCAAGT GGGGTATTCG TCTTGGTAAA CTTGGTATTT CTTTTCAGCC TGCTTTTAGA
    CAAAATTTTC TCACCAAGAA AGACCTTATC AAAGCTCTTG TGAATAACAT AGACATGGAC
    ACCAGCCTCA TTCTGGAGTA TTGCAGCACA TTTCAGCTGG ACTCTGACGC TGCCCTTCGG
    CTGTTCATTG AAACTCTGCT CCGCAACACT AGCAGCCAGA GCCAGGGAGA TGCTGCCCCG
    GAGTCCACAA AGCATCAGCA TTCCAAACTC CTGGCGAAAG CCACTGAGCT GGTCCCTTTA
    CTGAAGAACA CAAAGGATCT GGTGATCAGT CTTAGTGAGA TCCTCTATAA GCTGGACCCA
    TATGATTATG AAATGATTGA TGTTGTCCTG AAAGTTTTAG AACAAGCTAA CGAAAAGATA
    ACCAGTGTGA ACATCAATCA GGCGCTGAAT CTTCTGAGAC ACTTGAAGTC ATACAGAAGG
    ATCTCCCCGC CAGTGGACCA CGAATACCAG TACGCGCTGG AGCACATGAT CACTCTGCCA
    CCGGCTGCTC ACACTAGACT GCCCTTTCAC CTAATACTGT TTGGCACAGC ACAGAACTTC
    TGGAAAATTC TCTCCTCAGA GCTCAGCGAA GAGTCGCTGC CAACCCTGCT CTTAATCGCG
    AAGCTGATGA AGTTCTCCCT AGATACTCTC TATGTGTCTA CCGCCAAACA CTTGTTTGAA
    AAAAACCTGA AGCCAAAGCT CCTGAAGTCG GCACAAGCCA GGTCCTCCAC CCTGATGAGC
    AAGGAAGTGG ATAAGCTCAT GCAGACGCTG GAGTCGTACC TCCTGTCCAT CGTCAACCCC
    GAGTGGGCTG TCGCCATTGC CATCAGCCTC ACCCAGGAAG TCCCAGAAGG CCCCTTCAAG
    ATGTCTAGTT TGAAGTTCTG CCTGTATTTA GCTGAGAGAT GGCTACAAAA CATCCCACCT
    CAGGATGAGA CGTGTGAAAA GGCCAAAGCC TTGCAGAAGA AGCTCTGCCT CCAGGTCCGG
    CTCTCAGGAA CGGAAGCCGT GCTCATAGCC CACAAGCTGA ACGATCAGGA ATATTTAAGA
    GTGATAGGGA AGCCAGCGCA CCTCATCGTG AGCCTCTACG AACATCCCAG CATAAGCGAA
    AGGCTTTGTA CCACATCTGG CAAAGATTAT CCCGATATCC ATACAGCCGC TAAAGAAATA
    GCCGAAGTCA ATGAAGTTAA TTTGGAAAAA ATCTGGGACA TGTTGTTGGA GAAATGGCTG
    TGCCCCTCAA CGGTGCCCAG TGAGAAAGCA TCGGAGTTCT TTGAGCTGGA AGAGGATGAA
    GTGCTGCATA GAGTCGTGTA TCTCCTCCAG GCTCGTCCTG TTGATTATTG TTCGAGAATG
    CTGTTTGTAT TTGCCACTTC AGCTACAAGT ACACTGGGCA TGCGCCAGCT AACGTTTGCC
    CACAAAGCAC GAGCTCTGCA GTGTCTCCTC TATTTGGCTG ACAAGGAAAC AATTGAATCT
    CTCTTCAAAA AGCCCATTAA AGAAATGAAA TCCTATCTGA AATGTATAAC TTTCCTGGCA
    TCATTTGAGA CATTGAATAT TCCCATCACA TATGAATTAT TCTGCAACAG TCCCAAAGAA
    GGCATGATTA AGGGCTTGTG GAAGAACCAC AGCCACGAAC CTATGGCAGT ACGATTGGTT
    GCAGAGCTTT GCTTAGAATA CAAAATCTAT GACCTGCAGC TTTGGAATGG GCTCTTGCAG
    AAGCTTCTGG GCTTCAACAT GATTCCTTAC CTGAGGAAAG TGTTATCCTG CATTTCTAGC
    ATCCATTCGT TATGGCAGGT GCCCTACTTC AGCAAAGCGT GGCAGCGCGT GATCCAGATC
    CCTCTGCTCT CGGCTTCCTG TCCTTTAAGA CCCAGTCAGT TAGCAGACTG CTGTGACAGT
    CTCGTCGCCA TCCTTGAATG TCCAGTTTCA GATGACCTGG ACATGATGGG AGTCGCCAAG
    CAGTACGTCC AGTTAGACCT TCCCGCTTTT GCTCTGACCT GCCTGACGCT CATGCCCCAC
    TCTGAAAAAA GACACCAGCA GATCAAGAAT TTTCTGAATT CCTGTGATGC TCGGATCATT
    TTACAGCAGA TAGAAGAACA TATGAACACT GGCCAGCTAG CAGGGTTTTC ACATCAGATT
    GGAAGTCTGG TTCTGAATCA TGTTGTAAAT AAGAAGGAAT TTGGGATTTT GGCAAAGACA
    AAGTATTTTC AGTTGTTGAA ATGCCACGTG ATAAACACCG GCAACGTCAC TGAATTGGTA
    AACTATTTGG CAAATGACTT CAGTGTAGAT GAAGCATCAG CGTTGATAAA TGAGTATTCA
    AAGCATTGTG GGAAGCCTGT GCCAGCAGAT GCAGCTCCCT GTGAGATCCT GCAGACATTT
    CTTGGTGGAT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35847
    Clone ID Related Accession (Same CDS sequence) XM_006530252.3 , XM_006530252.4
    Accession Version XM_006530252.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4170bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product kinetochore-associated protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530252.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGAACA ATATTGAGCT GCTAACAAGC GATGACACTG GAAGTGGATG CCTGAGTGTT 
    GGCTCAAGAA AAGAAAACGT GACTGCACTG TACCAAGCCG ACTTGCTTGG GAAGATCTCC
    TCTGAGAAGA CCTCACTGAG TCCGAAGATC CAAGCATTCA GCTTAAGCCA TGGGTTTATA
    ATCGTTGCAG ACCAGTCAGT GATATTGCTT GACAGTATAT GCAGATCACT CCAGTTGTTT
    CTTATCTTCG ATACTGACGT GGATGTGGTC GGCCTGTGTC AAGAAGGAAA GTTTCTCTTT
    GTGGGGGAGA GAAGCGGCAA CTTCCACCTT ATCTACGTCA CATCGAAGCA AACGCTGTTC
    ACCAAGGCTT TTGTGGAGAA AGCTTTGGAC GAGAGTCAGC GAACGTACCG GAATCTCATT
    ATTGAGAAAG ATGGTTCAAA TGAAGGTACC TATTATATGT TGCTTCTAAC GAACAATGGA
    TTTTTTTATA TTACAAACCT CCAGCTTTCA CAAATTGAAC AGGCCATTGA GAACACGGAC
    TTGGATTCAG CCAAGAAGTT ACAAGGACAA TTCAAATGTA GTTTTATTTC TACTGAAAAT
    TACCACAGTT GTCTCAGTCT TGTGGCATCT CAATCTGGAA CATTTGCAAG TAAAACTTCT
    GTGATAATTG GGGGAACCGG TAGTTGTGCA TTCTCAAAAT GGGAACCCGA TTCTACCAAG
    AAAGAAATGT CTCTTAAGAA CTTTGTGGGC ACAGACATTA TTAAAGGTGC AAAGTCATTT
    CAGCTGATCG ACAACCTGCT TTTTGTGCTG GATACTGATA ATGTGCTGAG CTTGTGGGAT
    GCTTACACTC TCACTCCTGT GTGGAACTGG CCCTCTCTTC CCGTAGAGCA GTTTGTTCTC
    ACGACAGAAG CGGATTCTCC CTCATCTGTT ACCTGGCAAG GAATTACAAA TCTCAAATTA
    GTCACCCTGA CAGCAACAGC GAAGGAGAAG ATGAGAAGCC TCATCATTTA CTCTCTGCCT
    TCGATGGAAA CACTGTACTC TTTGGAAGTA TCTAGTGTTT CTTCTTTAGT TCAAACAGGA
    ATCAGCACGG ACACTATATA TCTTCTGGAA GGAATTCACA AAAATGATCC TAACCTATGT
    GAAGACTCTG TGTCTGACTT AGTACTTAGG TACCTCACAG AGGTGTTACC AGAAAACAGA
    CTGAGCCGGT TACTTCACAA GCACAGATTT GCCGAAGCCG AGAGCTTTGC CATTCAGTTC
    GGACTGGATG TTGAGCTTGT TTATAAGGTC AAATCAAATG ATATGTTGGA GAAGCTGGCT
    TTGATTTCCT CGGACAAGAG TGAGCAGTCC AAATGGCAGC AACTTGTAGA TGAAGCTAAA
    GAAAACCTAT GCAAAATCCA GGACGATGAC TTCGTGGTGA ATTTCTGCCT CAAGGCCCAA
    TGGGTAACCT ATGAAACCAC CCAGGAAATG CTCAGTTATG CCAAAACCAG GCTCATGAAG
    AAAGAAGACA GAGCTCTACC TGCCTCTTCT GATGCCTTCA TGGAGGTGCT AAAAGCTCAT
    GCAAAGTTAA CTACATTTTA TGGAGCATTT GGACCAGAAA AATTCAGTGG TAGCTCTTGG
    ATTGAGTTCC TGAATAACGA AGATGACCTC AGGGATGTTT TTTTGCAGCT AAGTGAAGGA
    AACTTCGCTT GTGCTCAGTA TCTTTGGCTT AGACATCGGG CTGACTTTGA AAGCAAGTTT
    GACGTGAAAA TGCTGGAGAA TTTGCTGAAT TCAATATCTA CACAATTCCC CTTGGAAAAC
    CTTTGTTCCT GGTTTAAAAA TGAAGTGATT CCGTTTGTGA GGAGGATTGT GCCCGAAGGA
    CAAAACATTC TTGCAAAATG GCTAGAACAA GCATCAAGGA ACCTAGAGTT AACTGATAAG
    GCAAACTGGC CAGAAAATGG ACTTCAGCTG GCAGAGGTGT TCTTCACTGC AGAAAAAACA
    GACCGGTTTG GATTCGCATC TTCTTGGCAC TGGATTTCCT TGGATTATCA AAACACAGAG
    GAAGTCCGCC AGTTACGGAC ACTGGTGAGC AAGTTGCGGG AGCTGATCAT TCTGCATAGG
    AAATACAACT GCAAGCTGGC CCTCTCGGAC TTTGAGAAGG AAAATGCCAC CACTGTGGTG
    TTCCGCATGT TCGACAGGGT CTCGGCCCCA GAGCTGATCC CTTCGGTCTT AGAGAAGTCT
    GTGAGAGTTT ACATTCGCGA ACAGAATCTG CAAGAGGAGG AGCTGCTGTT GCTCTATATA
    GAGGATTTAC TGAAGAGGTG TAGCTCAAAG TCAATGACTC TCTTCGACAC AGCGTGGGAA
    GCAAAGGCCA TGGCCGTGAT AAGATGCTTG TCTGACACAG ATCTCATCTT TGATGCTGTG
    CTGAAGATAA TGTACAAGGC GGTGGTTCCC TGGAGTGCAG CCGTGGAGCA GCTGGTGAAA
    CAGCACCTGG AAATGGACCA CCCCAAAGTC AAGTTATTGC AGGAAAGCTA CAAACTGATG
    GAGATGAAGA AACTTTTGCG AGGGTATGGG ATAAGAGAGG TGAATCTCCT GAACAAGGAA
    ATAATGAGAG TGATCCGATA CATTCTAAAG CAGGACATCC CGTCTTCTTT AGAAGATGCT
    CTGAAGGTGG CCCAGGGGTA CAGGCTGTCT GATGACGAAA TCTACAGCCT GAGAATCATC
    GATCTGATTG ACAGGGAGCA GGGGGGAGAT TGCCTCCTGC TACTGAAGTC TCTGCCTGCC
    GCTGAAGCCG AGAAGACTGC AGAAAGGGTT ATCATCTGGG CTCGACTGGC ACTGCAGGAG
    GAGCCTGACG GCTCTGAGGA GGACAAGGCC TGGAGAATAT CTGTGGCGAA GACATCGGTG
    GATATTCTCA AGATCCTGTG TGATATTCGA AAAGACAATC TGCAGAAGAA GGATGAATCT
    GAAGAATTCC TGAAGCGATT TCAAATGGTT GCTAGTTTAC AGGAGAACTT TGAGGTCTTC
    CTTCCATTTG AAGACTACAG CAACACCGCC TTGGTAGCTG GCCTTCGAGA ACAGTATATT
    AAGGCACAGG AAGCTGCACA GGCTGAGCAC AAACACAGGG GACCCCCCGG GCCCACCCCG
    GCTAGGGGGA CACACCTGAG CATCAAGTCA AAGCTGCACC GACAGGCACT GGCCCTGCAG
    GTGTCTGAAC AGGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAGGA CGGGAAGGTG
    GTGGCCGCCC TGAGCAAATG CAGAGATTTA CTTAAGCATT ACTGCAATGC CGACACTGGG
    AGGTTACTGT TTGTGGTGTG TCAGAAGCTC TGTCAGATGT TGGCTGACGA TGTCCCAATG
    GTGGCCCCAG GAGGACTCAG CCTTCCTTCT GAGATCCATG ACCTCGCATG CCACGCTGTC
    ACCATCTGCA GTCCAGATTA TTTGCTGGAT GTTTTAGAAC TAAGTAAATA CACTTTAACC
    GCTGTGGAGC TTTGCAGACA ATGCCAGATG GATGACTGTG GAATGCTGAT GAAGGCCGCT
    CTTGGAACTC ACAAGGATCC CTATGAAGAG TGGTCCTTTA GTGACTTCTT CAGTGAAGAT
    GGAATTGTTC TCGAGTCCCA GGTGGTGCTG CCTGTCATCT ATGAATTGAT CTCATCAGTG
    ATGCCCCCTG CAGAAAGCAA AAGACATCCT TTGGATTCTA TAAGTTTGCC ATACTGCTCC
    ACTAGTGAAG GAGAAAACCG TATTCTCCCT CTTGTAAGCT CCATCTCTGC TCTGCTCCGG
    AGCCTCCAGG AATGTAGTCA GTGGGAGCTA GCCCTGAGGT TCGTGGTGGG CTCCTTTGGT
    ACCTGCCTGC AGCACTCTAT GTCAAACGTC ATGAGTATCA GCTTGAGTAA ACAGTTACTT
    GGGAAGAACA CATTGGCGAA CTCCAGGCAT ATCATTATGG AATTGAAAGA GAAGTCCATC
    ACATTTATCA GAGAAAATGC TACGACACTC CTGCATAAAG TGTTTAACTG TCGGGTGGTG
    GATCTGGACC TCGCCTTGGC TTACTGCACT CTCTTACCTC AAAAGGATGT ATTCGACAAC
    CTCTGGAAGT TCATAGATAA AGCGTGGCAG AATTACGACA AAATTCTGGT ACGCCCTGAG
    GGAGGAGACC TGCTCCTGTT GCCATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006530315.1
    CDS181..4350
    Translation

    Target ORF information:

    RefSeq Version XM_006530252.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus kinetochore associated 1 (Kntc1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006530252.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGAACA ATATTGAGCT GCTAACAAGC GATGACACTG GAAGTGGATG CCTGAGTGTT 
    GGCTCAAGAA AAGAAAACGT GACTGCACTG TACCAAGCCG ACTTGCTTGG GAAGATCTCC
    TCTGAGAAGA CCTCACTGAG TCCGAAGATC CAAGCATTCA GCTTAAGCCA TGGGTTTATA
    ATCGTTGCAG ACCAGTCAGT GATATTGCTT GACAGTATAT GCAGATCACT CCAGTTGTTT
    CTTATCTTCG ATACTGACGT GGATGTGGTC GGCCTGTGTC AAGAAGGAAA GTTTCTCTTT
    GTGGGGGAGA GAAGCGGCAA CTTCCACCTT ATCTACGTCA CATCGAAGCA AACGCTGTTC
    ACCAAGGCTT TTGTGGAGAA AGCTTTGGAC GAGAGTCAGC GAACGTACCG GAATCTCATT
    ATTGAGAAAG ATGGTTCAAA TGAAGGTACC TATTATATGT TGCTTCTAAC GAACAATGGA
    TTTTTTTATA TTACAAACCT CCAGCTTTCA CAAATTGAAC AGGCCATTGA GAACACGGAC
    TTGGATTCAG CCAAGAAGTT ACAAGGACAA TTCAAATGTA GTTTTATTTC TACTGAAAAT
    TACCACAGTT GTCTCAGTCT TGTGGCATCT CAATCTGGAA CATTTGCAAG TAAAACTTCT
    GTGATAATTG GGGGAACCGG TAGTTGTGCA TTCTCAAAAT GGGAACCCGA TTCTACCAAG
    AAAGAAATGT CTCTTAAGAA CTTTGTGGGC ACAGACATTA TTAAAGGTGC AAAGTCATTT
    CAGCTGATCG ACAACCTGCT TTTTGTGCTG GATACTGATA ATGTGCTGAG CTTGTGGGAT
    GCTTACACTC TCACTCCTGT GTGGAACTGG CCCTCTCTTC CCGTAGAGCA GTTTGTTCTC
    ACGACAGAAG CGGATTCTCC CTCATCTGTT ACCTGGCAAG GAATTACAAA TCTCAAATTA
    GTCACCCTGA CAGCAACAGC GAAGGAGAAG ATGAGAAGCC TCATCATTTA CTCTCTGCCT
    TCGATGGAAA CACTGTACTC TTTGGAAGTA TCTAGTGTTT CTTCTTTAGT TCAAACAGGA
    ATCAGCACGG ACACTATATA TCTTCTGGAA GGAATTCACA AAAATGATCC TAACCTATGT
    GAAGACTCTG TGTCTGACTT AGTACTTAGG TACCTCACAG AGGTGTTACC AGAAAACAGA
    CTGAGCCGGT TACTTCACAA GCACAGATTT GCCGAAGCCG AGAGCTTTGC CATTCAGTTC
    GGACTGGATG TTGAGCTTGT TTATAAGGTC AAATCAAATG ATATGTTGGA GAAGCTGGCT
    TTGATTTCCT CGGACAAGAG TGAGCAGTCC AAATGGCAGC AACTTGTAGA TGAAGCTAAA
    GAAAACCTAT GCAAAATCCA GGACGATGAC TTCGTGGTGA ATTTCTGCCT CAAGGCCCAA
    TGGGTAACCT ATGAAACCAC CCAGGAAATG CTCAGTTATG CCAAAACCAG GCTCATGAAG
    AAAGAAGACA GAGCTCTACC TGCCTCTTCT GATGCCTTCA TGGAGGTGCT AAAAGCTCAT
    GCAAAGTTAA CTACATTTTA TGGAGCATTT GGACCAGAAA AATTCAGTGG TAGCTCTTGG
    ATTGAGTTCC TGAATAACGA AGATGACCTC AGGGATGTTT TTTTGCAGCT AAGTGAAGGA
    AACTTCGCTT GTGCTCAGTA TCTTTGGCTT AGACATCGGG CTGACTTTGA AAGCAAGTTT
    GACGTGAAAA TGCTGGAGAA TTTGCTGAAT TCAATATCTA CACAATTCCC CTTGGAAAAC
    CTTTGTTCCT GGTTTAAAAA TGAAGTGATT CCGTTTGTGA GGAGGATTGT GCCCGAAGGA
    CAAAACATTC TTGCAAAATG GCTAGAACAA GCATCAAGGA ACCTAGAGTT AACTGATAAG
    GCAAACTGGC CAGAAAATGG ACTTCAGCTG GCAGAGGTGT TCTTCACTGC AGAAAAAACA
    GACCGGTTTG GATTCGCATC TTCTTGGCAC TGGATTTCCT TGGATTATCA AAACACAGAG
    GAAGTCCGCC AGTTACGGAC ACTGGTGAGC AAGTTGCGGG AGCTGATCAT TCTGCATAGG
    AAATACAACT GCAAGCTGGC CCTCTCGGAC TTTGAGAAGG AAAATGCCAC CACTGTGGTG
    TTCCGCATGT TCGACAGGGT CTCGGCCCCA GAGCTGATCC CTTCGGTCTT AGAGAAGTCT
    GTGAGAGTTT ACATTCGCGA ACAGAATCTG CAAGAGGAGG AGCTGCTGTT GCTCTATATA
    GAGGATTTAC TGAAGAGGTG TAGCTCAAAG TCAATGACTC TCTTCGACAC AGCGTGGGAA
    GCAAAGGCCA TGGCCGTGAT AAGATGCTTG TCTGACACAG ATCTCATCTT TGATGCTGTG
    CTGAAGATAA TGTACAAGGC GGTGGTTCCC TGGAGTGCAG CCGTGGAGCA GCTGGTGAAA
    CAGCACCTGG AAATGGACCA CCCCAAAGTC AAGTTATTGC AGGAAAGCTA CAAACTGATG
    GAGATGAAGA AACTTTTGCG AGGGTATGGG ATAAGAGAGG TGAATCTCCT GAACAAGGAA
    ATAATGAGAG TGATCCGATA CATTCTAAAG CAGGACATCC CGTCTTCTTT AGAAGATGCT
    CTGAAGGTGG CCCAGGGGTA CAGGCTGTCT GATGACGAAA TCTACAGCCT GAGAATCATC
    GATCTGATTG ACAGGGAGCA GGGGGGAGAT TGCCTCCTGC TACTGAAGTC TCTGCCTGCC
    GCTGAAGCCG AGAAGACTGC AGAAAGGGTT ATCATCTGGG CTCGACTGGC ACTGCAGGAG
    GAGCCTGACG GCTCTGAGGA GGACAAGGCC TGGAGAATAT CTGTGGCGAA GACATCGGTG
    GATATTCTCA AGATCCTGTG TGATATTCGA AAAGACAATC TGCAGAAGAA GGATGAATCT
    GAAGAATTCC TGAAGCGATT TCAAATGGTT GCTAGTTTAC AGGAGAACTT TGAGGTCTTC
    CTTCCATTTG AAGACTACAG CAACACCGCC TTGGTAGCTG GCCTTCGAGA ACAGTATATT
    AAGGCACAGG AAGCTGCACA GGCTGAGCAC AAACACAGGG GACCCCCCGG GCCCACCCCG
    GCTAGGGGGA CACACCTGAG CATCAAGTCA AAGCTGCACC GACAGGCACT GGCCCTGCAG
    GTGTCTGAAC AGGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAGGA CGGGAAGGTG
    GTGGCCGCCC TGAGCAAATG CAGAGATTTA CTTAAGCATT ACTGCAATGC CGACACTGGG
    AGGTTACTGT TTGTGGTGTG TCAGAAGCTC TGTCAGATGT TGGCTGACGA TGTCCCAATG
    GTGGCCCCAG GAGGACTCAG CCTTCCTTCT GAGATCCATG ACCTCGCATG CCACGCTGTC
    ACCATCTGCA GTCCAGATTA TTTGCTGGAT GTTTTAGAAC TAAGTAAATA CACTTTAACC
    GCTGTGGAGC TTTGCAGACA ATGCCAGATG GATGACTGTG GAATGCTGAT GAAGGCCGCT
    CTTGGAACTC ACAAGGATCC CTATGAAGAG TGGTCCTTTA GTGACTTCTT CAGTGAAGAT
    GGAATTGTTC TCGAGTCCCA GGTGGTGCTG CCTGTCATCT ATGAATTGAT CTCATCAGTG
    ATGCCCCCTG CAGAAAGCAA AAGACATCCT TTGGATTCTA TAAGTTTGCC ATACTGCTCC
    ACTAGTGAAG GAGAAAACCG TATTCTCCCT CTTGTAAGCT CCATCTCTGC TCTGCTCCGG
    AGCCTCCAGG AATGTAGTCA GTGGGAGCTA GCCCTGAGGT TCGTGGTGGG CTCCTTTGGT
    ACCTGCCTGC AGCACTCTAT GTCAAACGTC ATGAGTATCA GCTTGAGTAA ACAGTTACTT
    GGGAAGAACA CATTGGCGAA CTCCAGGCAT ATCATTATGG AATTGAAAGA GAAGTCCATC
    ACATTTATCA GAGAAAATGC TACGACACTC CTGCATAAAG TGTTTAACTG TCGGGTGGTG
    GATCTGGACC TCGCCTTGGC TTACTGCACT CTCTTACCTC AAAAGGATGT ATTCGACAAC
    CTCTGGAAGT TCATAGATAA AGCGTGGCAG AATTACGACA AAATTCTGGT ACGCCCTGAG
    GGAGGAGACC TGCTCCTGTT GCCATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35844
    Clone ID Related Accession (Same CDS sequence) XM_006530249.1 , XM_030254330.1
    Accession Version XM_030254330.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6168bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product kinetochore-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTTGCTTC TAACGAACAA TGGATTTTTT TATATTACAA ACCTCCAGCT TTCACAAATT 
    GAACAGGCCA TTGAGAACAC GGACTTGGAT TCAGCCAAGA AGTTACAAGG ACAATTCAAA
    TGTAGTTTTA TTTCTACTGA AAATTACCAC AGTTGTCTCA GTCTTGTGGC ATCTCAATCT
    GGAACATTTG CAAGTAAAAC TTCTGTGATA ATTGGGGGAA CCGGTAGTTG TGCATTCTCA
    AAATGGGAAC CCGATTCTAC CAAGAAAGAA ATGTCTCTTA AGAACTTTGT GGGCACAGAC
    ATTATTAAAG GTGCAAAGTC ATTTCAGCTG ATCGACAACC TGCTTTTTGT GCTGGATACT
    GATAATGTGC TGAGCTTGTG GGATGCTTAC ACTCTCACTC CTGTGTGGAA CTGGCCCTCT
    CTTCCCGTAG AGCAGTTTGT TCTCACGACA GAAGCGGATT CTCCCTCATC TGTTACCTGG
    CAAGGAATTA CAAATCTCAA ATTAGTCACC CTGACAGCAA CAGCGAAGGA GAAGATGAGA
    AGCCTCATCA TTTACTCTCT GCCTTCGATG GAAACACTGT ACTCTTTGGA AGTATCTAGT
    GTTTCTTCTT TAGTTCAAAC AGGAATCAGC ACGGACACTA TATATCTTCT GGAAGGAATT
    CACAAAAATG ATCCTAACCT ATGTGAAGAC TCTGTGTCTG ACTTAGTACT TAGGTACCTC
    ACAGAGGTGT TACCAGAAAA CAGACTGAGC CGGTTACTTC ACAAGCACAG ATTTGCCGAA
    GCCGAGAGCT TTGCCATTCA GTTCGGACTG GATGTTGAGC TTGTTTATAA GGTCAAATCA
    AATGATATGT TGGAGAAGCT GGCTTTGATT TCCTCGGACA AGAGTGAGCA GTCCAAATGG
    CAGCAACTTG TAGATGAAGC TAAAGAAAAC CTATGCAAAA TCCAGGACGA TGACTTCGTG
    GTGAATTTCT GCCTCAAGGC CCAATGGGTA ACCTATGAAA CCACCCAGGA AATGCTCAGT
    TATGCCAAAA CCAGGCTCAT GAAGAAAGAA GACAGAGCTC TACCTGCCTC TTCTGATGCC
    TTCATGGAGG TGCTAAAAGC TCATGCAAAG TTAACTACAT TTTATGGAGC ATTTGGACCA
    GAAAAATTCA GTGGTAGCTC TTGGATTGAG TTCCTGAATA ACGAAGATGA CCTCAGGGAT
    GTTTTTTTGC AGCTAAGTGA AGGAAACTTC GCTTGTGCTC AGTATCTTTG GCTTAGACAT
    CGGGCTGACT TTGAAAGCAA GTTTGACGTG AAAATGCTGG AGAATTTGCT GAATTCAATA
    TCTACACAAT TCCCCTTGGA AAACCTTTGT TCCTGGTTTA AAAATGAAGT GATTCCGTTT
    GTGAGGAGGA TTGTGCCCGA AGGACAAAAC ATTCTTGCAA AATGGCTAGA ACAAGCATCA
    AGGAACCTAG AGTTAACTGA TAAGGCAAAC TGGCCAGAAA ATGGACTTCA GCTGGCAGAG
    GTGTTCTTCA CTGCAGAAAA AACAGACCGG TTTGGATTCG CATCTTCTTG GCACTGGATT
    TCCTTGGATT ATCAAAACAC AGAGGAAGTC CGCCAGTTAC GGACACTGGT GAGCAAGTTG
    CGGGAGCTGA TCATTCTGCA TAGGAAATAC AACTGCAAGC TGGCCCTCTC GGACTTTGAG
    AAGGAAAATG CCACCACTGT GGTGTTCCGC ATGTTCGACA GGGTCTCGGC CCCAGAGCTG
    ATCCCTTCGG TCTTAGAGAA GTCTGTGAGA GTTTACATTC GCGAACAGAA TCTGCAAGAG
    GAGGAGCTGC TGTTGCTCTA TATAGAGGAT TTACTGAAGA GGTGTAGCTC AAAGTCAATG
    ACTCTCTTCG ACACAGCGTG GGAAGCAAAG GCCATGGCCG TGATAAGATG CTTGTCTGAC
    ACAGATCTCA TCTTTGATGC TGTGCTGAAG ATAATGTACA AGGCGGTGGT TCCCTGGAGT
    GCAGCCGTGG AGCAGCTGGT GAAACAGCAC CTGGAAATGG ACCACCCCAA AGTCAAGTTA
    TTGCAGGAAA GCTACAAACT GATGGAGATG AAGAAACTTT TGCGAGGGTA TGGGATAAGA
    GAGGTGAATC TCCTGAACAA GGAAATAATG AGAGTGATCC GATACATTCT AAAGCAGGAC
    ATCCCGTCTT CTTTAGAAGA TGCTCTGAAG GTGGCCCAGG GGTACAGGCT GTCTGATGAC
    GAAATCTACA GCCTGAGAAT CATCGATCTG ATTGACAGGG AGCAGGGGGG AGATTGCCTC
    CTGCTACTGA AGTCTCTGCC TGCCGCTGAA GCCGAGAAGA CTGCAGAAAG GGTTATCATC
    TGGGCTCGAC TGGCACTGCA GGAGGAGCCT GACGGCTCTG AGGAGGACAA GGCCTGGAGA
    ATATCTGTGG CGAAGACATC GGTGGATATT CTCAAGATCC TGTGTGATAT TCGAAAAGAC
    AATCTGCAGA AGAAGGATGA ATCTGAAGAA TTCCTGAAGC GATTTCAAAT GGTTGCTAGT
    TTACAGGAGA ACTTTGAGGT CTTCCTTCCA TTTGAAGACT ACAGCAACAC CGCCTTGGTA
    GCTGGCCTTC GAGAACAGTA TATTAAGGCA CAGGAAGCTG CACAGGCTGA GCACAAACAC
    AGGGGACCCC CCGGGCCCAC CCCGGCTAGG GGGACACACC TGAGCATCAA GTCAAAGCTG
    CACCGACAGG CACTGGCCCT GCAGGTGTCT GAACAGGAGC TGGAGGCAGA GCTGACCTTG
    AGAGCCTTAA AGGACGGGAA GGTGGTGGCC GCCCTGAGCA AATGCAGAGA TTTACTTAAG
    CATTACTGCA ATGCCGACAC TGGGAGGTTA CTGTTTGTGG TGTGTCAGAA GCTCTGTCAG
    ATGTTGGCTG ACGATGTCCC AATGGTGGCC CCAGGAGGAC TCAGCCTTCC TTCTGAGATC
    CATGACCTCG CATGCCACGC TGTCACCATC TGCAGTCCAG ATTATTTGCT GGATGTTTTA
    GAACTAAGTA AATACACTTT AACCGCTGTG GAGCTTTGCA GACAATGCCA GATGGATGAC
    TGTGGAATGC TGATGAAGGC CGCTCTTGGA ACTCACAAGG ATCCCTATGA AGAGTGGTCC
    TTTAGTGACT TCTTCAGTGA AGATGGAATT GTTCTCGAGT CCCAGGTGGT GCTGCCTGTC
    ATCTATGAAT TGATCTCATC AGTGATGCCC CCTGCAGAAA GCAAAAGACA TCCTTTGGAT
    TCTATAAGTT TGCCATACTG CTCCACTAGT GAAGGAGAAA ACCGTATTCT CCCTCTTGTA
    AGCTCCATCT CTGCTCTGCT CCGGAGCCTC CAGGAATGTA GTCAGTGGGA GCTAGCCCTG
    AGGTTCGTGG TGGGCTCCTT TGGTACCTGC CTGCAGCACT CTATGTCAAA CGTCATGAGT
    ATCAGCTTGA GTAAACAGTT ACTTGGGAAG AACACATTGG CGAACTCCAG GCATATCATT
    ATGGAATTGA AAGAGAAGTC CATCACATTT ATCAGAGAAA ATGCTACGAC ACTCCTGCAT
    AAAGTGTTTA ACTGTCGGGT GGTGGATCTG GACCTCGCCT TGGCTTACTG CACTCTCTTA
    CCTCAAAAGG ATGTATTCGA CAACCTCTGG AAGTTCATAG ATAAAGCGTG GCAGAATTAC
    GACAAAATTC TGGCATTATC TTTGGTGGGC TCTCAGCTGG CAAATCTCTA CCAGGATATT
    GAAACAGGAC TTTGGTTCCA CGAGCTCAGT ATCGATGCCA AGTGGGGTAT TCGTCTTGGT
    AAACTTGGTA TTTCTTTTCA GCCTGCTTTT AGACAAAATT TTCTCACCAA GAAAGACCTT
    ATCAAAGCTC TTGTGAATAA CATAGACATG GACACCAGCC TCATTCTGGA GTATTGCAGC
    ACATTTCAGC TGGACTCTGA CGCTGCCCTT CGGCTGTTCA TTGAAACTCT GCTCCGCAAC
    ACTAGCAGCC AGAGCCAGGG AGATGCTGCC CCGGAGTCCA CAAAGCATCA GCATTCCAAA
    CTCCTGGCGA AAGCCACTGA GCTGGTCCCT TTACTGAAGA ACACAAAGGA TCTGGTGATC
    AGTCTTAGTG AGATCCTCTA TAAGCTGGAC CCATATGATT ATGAAATGAT TGATGTTGTC
    CTGAAAGTTT TAGAACAAGC TAACGAAAAG ATAACCAGTG TGAACATCAA TCAGGCGCTG
    AATCTTCTGA GACACTTGAA GTCATACAGA AGGATCTCCC CGCCAGTGGA CCACGAATAC
    CAGTACGCGC TGGAGCACAT GATCACTCTG CCACCGGCTG CTCACACTAG ACTGCCCTTT
    CACCTAATAC TGTTTGGCAC AGCACAGAAC TTCTGGAAAA TTCTCTCCTC AGAGCTCAGC
    GAAGAGTCGC TGCCAACCCT GCTCTTAATC GCGAAGCTGA TGAAGTTCTC CCTAGATACT
    CTCTATGTGT CTACCGCCAA ACACTTGTTT GAAAAAAACC TGAAGCCAAA GCTCCTGAAG
    TCGGCACAAG CCAGGTCCTC CACCCTGATG AGCAAGGAAG TGGATAAGCT CATGCAGACG
    CTGGAGTCGT ACCTCCTGTC CATCGTCAAC CCCGAGTGGG CTGTCGCCAT TGCCATCAGC
    CTCACCCAGG AAGTCCCAGA AGGCCCCTTC AAGATGTCTA GTTTGAAGTT CTGCCTGTAT
    TTAGCTGAGA GATGGCTACA AAACATCCCA CCTCAGGATG AGACGTGTGA AAAGGCCAAA
    GCCTTGCAGA AGAAGCTCTG CCTCCAGGTC CGGCTCTCAG GAACGGAAGC CGTGCTCATA
    GCCCACAAGC TGAACGATCA GGAATATTTA AGAGTGATAG GGAAGCCAGC GCACCTCATC
    GTGAGCCTCT ACGAACATCC CAGCATAAGC GAAAGGCTTT GTACCACATC TGGCAAAGAT
    TATCCCGATA TCCATACAGC CGCTAAAGAA ATAGCCGAAG TCAATGAAGT TAATTTGGAA
    AAAATCTGGG ACATGTTGTT GGAGAAATGG CTGTGCCCCT CAACGGTGCC CAGTGAGAAA
    GCATCGGAGT TCTTTGAGCT GGAAGAGGAT GAAGTGCTGC ATAGAGTCGT GTATCTCCTC
    CAGGCTCGTC CTGTTGATTA TTGTTCGAGA ATGCTGTTTG TATTTGCCAC TTCAGCTACA
    AGTACACTGG GCATGCGCCA GCTAACGTTT GCCCACAAAG CACGAGCTCT GCAGTGTCTC
    CTCTATTTGG CTGACAAGGA AACAATTGAA TCTCTCTTCA AAAAGCCCAT TAAAGAAATG
    AAATCCTATC TGAAATGTAT AACTTTCCTG GCATCATTTG AGACATTGAA TATTCCCATC
    ACATATGAAT TATTCTGCAA CAGTCCCAAA GAAGGCATGA TTAAGGGCTT GTGGAAGAAC
    CACAGCCACG AACCTATGGC AGTACGATTG GTTGCAGAGC TTTGCTTAGA ATACAAAATC
    TATGACCTGC AGCTTTGGAA TGGGCTCTTG CAGAAGCTTC TGGGCTTCAA CATGATTCCT
    TACCTGAGGA AAGTGTTATC CTGCATTTCT AGCATCCATT CGTTATGGCA GGTGCCCTAC
    TTCAGCAAAG CGTGGCAGCG CGTGATCCAG ATCCCTCTGC TCTCGGCTTC CTGTCCTTTA
    AGACCCAGTC AGTTAGCAGA CTGCTGTGAC AGTCTCGTCG CCATCCTTGA ATGTCCAGTT
    TCAGATGACC TGGACATGAT GGGAGTCGCC AAGCAGTACG TCCAGTTAGA CCTTCCCGCT
    TTTGCTCTGA CCTGCCTGAC GCTCATGCCC CACTCTGAAA AAAGACACCA GCAGATCAAG
    AATTTTCTGA ATTCCTGTGA TGCTCGGATC ATTTTACAGC AGATAGAAGA ACATATGAAC
    ACTGGCCAGC TAGCAGGGTT TTCACATCAG ATTGGAAGTC TGGTTCTGAA TCATGTTGTA
    AATAAGAAGG AATTTGGGAT TTTGGCAAAG ACAAAGTATT TTCAGTTGTT GAAATGCCAC
    GTGATAAACA CCGGCAACGT CACTGAATTG GTAAACTATT TGGCAAATGA CTTCAGTGTA
    GATGAAGCAT CAGCGTTGAT AAATGAGTAT TCAAAGCATT GTGGGAAGCC TGTGCCAGCA
    GATGCAGCTC CCTGTGAGAT CCTGCAGACA TTTCTTGGTG GATCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030110190.1
    CDS510..6677
    Translation

    Target ORF information:

    RefSeq Version XM_030254330.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus kinetochore associated 1 (Kntc1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030254330.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTTGCTTC TAACGAACAA TGGATTTTTT TATATTACAA ACCTCCAGCT TTCACAAATT 
    GAACAGGCCA TTGAGAACAC GGACTTGGAT TCAGCCAAGA AGTTACAAGG ACAATTCAAA
    TGTAGTTTTA TTTCTACTGA AAATTACCAC AGTTGTCTCA GTCTTGTGGC ATCTCAATCT
    GGAACATTTG CAAGTAAAAC TTCTGTGATA ATTGGGGGAA CCGGTAGTTG TGCATTCTCA
    AAATGGGAAC CCGATTCTAC CAAGAAAGAA ATGTCTCTTA AGAACTTTGT GGGCACAGAC
    ATTATTAAAG GTGCAAAGTC ATTTCAGCTG ATCGACAACC TGCTTTTTGT GCTGGATACT
    GATAATGTGC TGAGCTTGTG GGATGCTTAC ACTCTCACTC CTGTGTGGAA CTGGCCCTCT
    CTTCCCGTAG AGCAGTTTGT TCTCACGACA GAAGCGGATT CTCCCTCATC TGTTACCTGG
    CAAGGAATTA CAAATCTCAA ATTAGTCACC CTGACAGCAA CAGCGAAGGA GAAGATGAGA
    AGCCTCATCA TTTACTCTCT GCCTTCGATG GAAACACTGT ACTCTTTGGA AGTATCTAGT
    GTTTCTTCTT TAGTTCAAAC AGGAATCAGC ACGGACACTA TATATCTTCT GGAAGGAATT
    CACAAAAATG ATCCTAACCT ATGTGAAGAC TCTGTGTCTG ACTTAGTACT TAGGTACCTC
    ACAGAGGTGT TACCAGAAAA CAGACTGAGC CGGTTACTTC ACAAGCACAG ATTTGCCGAA
    GCCGAGAGCT TTGCCATTCA GTTCGGACTG GATGTTGAGC TTGTTTATAA GGTCAAATCA
    AATGATATGT TGGAGAAGCT GGCTTTGATT TCCTCGGACA AGAGTGAGCA GTCCAAATGG
    CAGCAACTTG TAGATGAAGC TAAAGAAAAC CTATGCAAAA TCCAGGACGA TGACTTCGTG
    GTGAATTTCT GCCTCAAGGC CCAATGGGTA ACCTATGAAA CCACCCAGGA AATGCTCAGT
    TATGCCAAAA CCAGGCTCAT GAAGAAAGAA GACAGAGCTC TACCTGCCTC TTCTGATGCC
    TTCATGGAGG TGCTAAAAGC TCATGCAAAG TTAACTACAT TTTATGGAGC ATTTGGACCA
    GAAAAATTCA GTGGTAGCTC TTGGATTGAG TTCCTGAATA ACGAAGATGA CCTCAGGGAT
    GTTTTTTTGC AGCTAAGTGA AGGAAACTTC GCTTGTGCTC AGTATCTTTG GCTTAGACAT
    CGGGCTGACT TTGAAAGCAA GTTTGACGTG AAAATGCTGG AGAATTTGCT GAATTCAATA
    TCTACACAAT TCCCCTTGGA AAACCTTTGT TCCTGGTTTA AAAATGAAGT GATTCCGTTT
    GTGAGGAGGA TTGTGCCCGA AGGACAAAAC ATTCTTGCAA AATGGCTAGA ACAAGCATCA
    AGGAACCTAG AGTTAACTGA TAAGGCAAAC TGGCCAGAAA ATGGACTTCA GCTGGCAGAG
    GTGTTCTTCA CTGCAGAAAA AACAGACCGG TTTGGATTCG CATCTTCTTG GCACTGGATT
    TCCTTGGATT ATCAAAACAC AGAGGAAGTC CGCCAGTTAC GGACACTGGT GAGCAAGTTG
    CGGGAGCTGA TCATTCTGCA TAGGAAATAC AACTGCAAGC TGGCCCTCTC GGACTTTGAG
    AAGGAAAATG CCACCACTGT GGTGTTCCGC ATGTTCGACA GGGTCTCGGC CCCAGAGCTG
    ATCCCTTCGG TCTTAGAGAA GTCTGTGAGA GTTTACATTC GCGAACAGAA TCTGCAAGAG
    GAGGAGCTGC TGTTGCTCTA TATAGAGGAT TTACTGAAGA GGTGTAGCTC AAAGTCAATG
    ACTCTCTTCG ACACAGCGTG GGAAGCAAAG GCCATGGCCG TGATAAGATG CTTGTCTGAC
    ACAGATCTCA TCTTTGATGC TGTGCTGAAG ATAATGTACA AGGCGGTGGT TCCCTGGAGT
    GCAGCCGTGG AGCAGCTGGT GAAACAGCAC CTGGAAATGG ACCACCCCAA AGTCAAGTTA
    TTGCAGGAAA GCTACAAACT GATGGAGATG AAGAAACTTT TGCGAGGGTA TGGGATAAGA
    GAGGTGAATC TCCTGAACAA GGAAATAATG AGAGTGATCC GATACATTCT AAAGCAGGAC
    ATCCCGTCTT CTTTAGAAGA TGCTCTGAAG GTGGCCCAGG GGTACAGGCT GTCTGATGAC
    GAAATCTACA GCCTGAGAAT CATCGATCTG ATTGACAGGG AGCAGGGGGG AGATTGCCTC
    CTGCTACTGA AGTCTCTGCC TGCCGCTGAA GCCGAGAAGA CTGCAGAAAG GGTTATCATC
    TGGGCTCGAC TGGCACTGCA GGAGGAGCCT GACGGCTCTG AGGAGGACAA GGCCTGGAGA
    ATATCTGTGG CGAAGACATC GGTGGATATT CTCAAGATCC TGTGTGATAT TCGAAAAGAC
    AATCTGCAGA AGAAGGATGA ATCTGAAGAA TTCCTGAAGC GATTTCAAAT GGTTGCTAGT
    TTACAGGAGA ACTTTGAGGT CTTCCTTCCA TTTGAAGACT ACAGCAACAC CGCCTTGGTA
    GCTGGCCTTC GAGAACAGTA TATTAAGGCA CAGGAAGCTG CACAGGCTGA GCACAAACAC
    AGGGGACCCC CCGGGCCCAC CCCGGCTAGG GGGACACACC TGAGCATCAA GTCAAAGCTG
    CACCGACAGG CACTGGCCCT GCAGGTGTCT GAACAGGAGC TGGAGGCAGA GCTGACCTTG
    AGAGCCTTAA AGGACGGGAA GGTGGTGGCC GCCCTGAGCA AATGCAGAGA TTTACTTAAG
    CATTACTGCA ATGCCGACAC TGGGAGGTTA CTGTTTGTGG TGTGTCAGAA GCTCTGTCAG
    ATGTTGGCTG ACGATGTCCC AATGGTGGCC CCAGGAGGAC TCAGCCTTCC TTCTGAGATC
    CATGACCTCG CATGCCACGC TGTCACCATC TGCAGTCCAG ATTATTTGCT GGATGTTTTA
    GAACTAAGTA AATACACTTT AACCGCTGTG GAGCTTTGCA GACAATGCCA GATGGATGAC
    TGTGGAATGC TGATGAAGGC CGCTCTTGGA ACTCACAAGG ATCCCTATGA AGAGTGGTCC
    TTTAGTGACT TCTTCAGTGA AGATGGAATT GTTCTCGAGT CCCAGGTGGT GCTGCCTGTC
    ATCTATGAAT TGATCTCATC AGTGATGCCC CCTGCAGAAA GCAAAAGACA TCCTTTGGAT
    TCTATAAGTT TGCCATACTG CTCCACTAGT GAAGGAGAAA ACCGTATTCT CCCTCTTGTA
    AGCTCCATCT CTGCTCTGCT CCGGAGCCTC CAGGAATGTA GTCAGTGGGA GCTAGCCCTG
    AGGTTCGTGG TGGGCTCCTT TGGTACCTGC CTGCAGCACT CTATGTCAAA CGTCATGAGT
    ATCAGCTTGA GTAAACAGTT ACTTGGGAAG AACACATTGG CGAACTCCAG GCATATCATT
    ATGGAATTGA AAGAGAAGTC CATCACATTT ATCAGAGAAA ATGCTACGAC ACTCCTGCAT
    AAAGTGTTTA ACTGTCGGGT GGTGGATCTG GACCTCGCCT TGGCTTACTG CACTCTCTTA
    CCTCAAAAGG ATGTATTCGA CAACCTCTGG AAGTTCATAG ATAAAGCGTG GCAGAATTAC
    GACAAAATTC TGGCATTATC TTTGGTGGGC TCTCAGCTGG CAAATCTCTA CCAGGATATT
    GAAACAGGAC TTTGGTTCCA CGAGCTCAGT ATCGATGCCA AGTGGGGTAT TCGTCTTGGT
    AAACTTGGTA TTTCTTTTCA GCCTGCTTTT AGACAAAATT TTCTCACCAA GAAAGACCTT
    ATCAAAGCTC TTGTGAATAA CATAGACATG GACACCAGCC TCATTCTGGA GTATTGCAGC
    ACATTTCAGC TGGACTCTGA CGCTGCCCTT CGGCTGTTCA TTGAAACTCT GCTCCGCAAC
    ACTAGCAGCC AGAGCCAGGG AGATGCTGCC CCGGAGTCCA CAAAGCATCA GCATTCCAAA
    CTCCTGGCGA AAGCCACTGA GCTGGTCCCT TTACTGAAGA ACACAAAGGA TCTGGTGATC
    AGTCTTAGTG AGATCCTCTA TAAGCTGGAC CCATATGATT ATGAAATGAT TGATGTTGTC
    CTGAAAGTTT TAGAACAAGC TAACGAAAAG ATAACCAGTG TGAACATCAA TCAGGCGCTG
    AATCTTCTGA GACACTTGAA GTCATACAGA AGGATCTCCC CGCCAGTGGA CCACGAATAC
    CAGTACGCGC TGGAGCACAT GATCACTCTG CCACCGGCTG CTCACACTAG ACTGCCCTTT
    CACCTAATAC TGTTTGGCAC AGCACAGAAC TTCTGGAAAA TTCTCTCCTC AGAGCTCAGC
    GAAGAGTCGC TGCCAACCCT GCTCTTAATC GCGAAGCTGA TGAAGTTCTC CCTAGATACT
    CTCTATGTGT CTACCGCCAA ACACTTGTTT GAAAAAAACC TGAAGCCAAA GCTCCTGAAG
    TCGGCACAAG CCAGGTCCTC CACCCTGATG AGCAAGGAAG TGGATAAGCT CATGCAGACG
    CTGGAGTCGT ACCTCCTGTC CATCGTCAAC CCCGAGTGGG CTGTCGCCAT TGCCATCAGC
    CTCACCCAGG AAGTCCCAGA AGGCCCCTTC AAGATGTCTA GTTTGAAGTT CTGCCTGTAT
    TTAGCTGAGA GATGGCTACA AAACATCCCA CCTCAGGATG AGACGTGTGA AAAGGCCAAA
    GCCTTGCAGA AGAAGCTCTG CCTCCAGGTC CGGCTCTCAG GAACGGAAGC CGTGCTCATA
    GCCCACAAGC TGAACGATCA GGAATATTTA AGAGTGATAG GGAAGCCAGC GCACCTCATC
    GTGAGCCTCT ACGAACATCC CAGCATAAGC GAAAGGCTTT GTACCACATC TGGCAAAGAT
    TATCCCGATA TCCATACAGC CGCTAAAGAA ATAGCCGAAG TCAATGAAGT TAATTTGGAA
    AAAATCTGGG ACATGTTGTT GGAGAAATGG CTGTGCCCCT CAACGGTGCC CAGTGAGAAA
    GCATCGGAGT TCTTTGAGCT GGAAGAGGAT GAAGTGCTGC ATAGAGTCGT GTATCTCCTC
    CAGGCTCGTC CTGTTGATTA TTGTTCGAGA ATGCTGTTTG TATTTGCCAC TTCAGCTACA
    AGTACACTGG GCATGCGCCA GCTAACGTTT GCCCACAAAG CACGAGCTCT GCAGTGTCTC
    CTCTATTTGG CTGACAAGGA AACAATTGAA TCTCTCTTCA AAAAGCCCAT TAAAGAAATG
    AAATCCTATC TGAAATGTAT AACTTTCCTG GCATCATTTG AGACATTGAA TATTCCCATC
    ACATATGAAT TATTCTGCAA CAGTCCCAAA GAAGGCATGA TTAAGGGCTT GTGGAAGAAC
    CACAGCCACG AACCTATGGC AGTACGATTG GTTGCAGAGC TTTGCTTAGA ATACAAAATC
    TATGACCTGC AGCTTTGGAA TGGGCTCTTG CAGAAGCTTC TGGGCTTCAA CATGATTCCT
    TACCTGAGGA AAGTGTTATC CTGCATTTCT AGCATCCATT CGTTATGGCA GGTGCCCTAC
    TTCAGCAAAG CGTGGCAGCG CGTGATCCAG ATCCCTCTGC TCTCGGCTTC CTGTCCTTTA
    AGACCCAGTC AGTTAGCAGA CTGCTGTGAC AGTCTCGTCG CCATCCTTGA ATGTCCAGTT
    TCAGATGACC TGGACATGAT GGGAGTCGCC AAGCAGTACG TCCAGTTAGA CCTTCCCGCT
    TTTGCTCTGA CCTGCCTGAC GCTCATGCCC CACTCTGAAA AAAGACACCA GCAGATCAAG
    AATTTTCTGA ATTCCTGTGA TGCTCGGATC ATTTTACAGC AGATAGAAGA ACATATGAAC
    ACTGGCCAGC TAGCAGGGTT TTCACATCAG ATTGGAAGTC TGGTTCTGAA TCATGTTGTA
    AATAAGAAGG AATTTGGGAT TTTGGCAAAG ACAAAGTATT TTCAGTTGTT GAAATGCCAC
    GTGATAAACA CCGGCAACGT CACTGAATTG GTAAACTATT TGGCAAATGA CTTCAGTGTA
    GATGAAGCAT CAGCGTTGAT AAATGAGTAT TCAAAGCATT GTGGGAAGCC TGTGCCAGCA
    GATGCAGCTC CCTGTGAGAT CCTGCAGACA TTTCTTGGTG GATCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35845
    Clone ID Related Accession (Same CDS sequence) XM_006530250.3 , XM_006530250.4
    Accession Version XM_006530250.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6099bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product kinetochore-associated protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530250.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTGGAACA ATATTGAGCT GCTAACAAGC GATGACACTG GAAGTGGATG CCTGAGTGTT 
    GGCTCAAGAA AAGAAAACGT GACTGCACTG TACCAAGCCG ACTTGCTTGG GAAGATCTCC
    TCTGAGAAGA CCTCACTGAG TCCGAAGATC CAAGCATTCA GCTTAAGCCA TGGGTTTATA
    ATCGTTGCAG ACCAGTCAGT GATATTGCTT GACAGTATAT GCAGATCACT CCAGTTGTTT
    CTTATCTTCG ATACTGACGT GGATGTGGTC GGCCTGTGTC AAGAAGGAAA GTTTCTCTTT
    GTGGGGGAGA GAAGCGGCAA CTTCCACCTT ATCTACGTCA CATCGAAGCA AACGCTGTTC
    ACCAAGGCTT TTGTGGAGAA AGCTTTGGAC GAGAGTCAGC GAACGTACCG GAATCTCATT
    ATTGAGAAAG ATGGTTCAAA TGAAGGTACC TATTATATGT TGCTTCTAAC GAACAATGGA
    TTTTTTTATA TTACAAACCT CCAGCTTTCA CAAATTGAAC AGGCCATTGA GAACACGGAC
    TTGGATTCAG CCAAGAAGTT ACAAGGACAA TTCAAATGTA GTTTTATTTC TACTGAAAAT
    TACCACAGTT GTCTCAGTCT TGTGGCATCT CAATCTGGAA CATTTGCAAG TAAAACTTCT
    GTGATAATTG GGGGAACCGG TAGTTGTGCA TTCTCAAAAT GGGAACCCGA TTCTACCAAG
    AAAGAAATGT CTCTTAAGAA CTTTGTGGGC ACAGACATTA TTAAAGGTGC AAAGTCATTT
    CAGCTGATCG ACAACCTGCT TTTTGTGCTG GATACTGATA ATGTGCTGAG CTTGTGGGAT
    GCTTACACTC TCACTCCTGT GTGGAACTGG CCCTCTCTTC CCGTAGAGCA GTTTGTTCTC
    ACGACAGAAG CGGATTCTCC CTCATCTGTT ACCTGGCAAG GAATTACAAA TCTCAAATTA
    GTCACCCTGA CAGCAACAGC GAAGGAGAAG ATGAGAAGCC TCATCATTTA CTCTCTGCCT
    TCGATGGAAA CACTGTACTC TTTGGAAGTA TCTAGTGTTT CTTCTTTAGT TCAAACAGGA
    ATCAGCACGG ACACTATATA TCTTCTGGAA GGAATTCACA AAAATGATCC TAACCTATGT
    GAAGACTCTG TGTCTGACTT AGTACTTAGG TACCTCACAG AGGTGTTACC AGAAAACAGA
    CTGAGCCGGT TACTTCACAA GCACAGATTT GCCGAAGCCG AGAGCTTTGC CATTCAGTTC
    GGACTGGATG TTGAGCTTGT TTATAAGGTC AAATCAAATG ATATGTTGGA GAAGCTGGCT
    TTGATTTCCT CGGACAAGAG TGAGCAGTCC AAATGGCAGC AACTTGTAGA TGAAGCTAAA
    GAAAACCTAT GCAAAATCCA GGACGATGAC TTCGTGGTGA ATTTCTGCCT CAAGGCCCAA
    TGGGTAACCT ATGAAACCAC CCAGGAAATG CTCAGTTATG CCAAAACCAG GCTCATGAAG
    AAAGAAGACA GAGCTCTACC TGCCTCTTCT GATGCCTTCA TGGAGGTGCT AAAAGCTCAT
    GCAAAGTTAA CTACATTTTA TGGAGCATTT GGACCAGAAA AATTCAGTGG TAGCTCTTGG
    ATTGAGTTCC TGAATAACGA AGATGACCTC AGGGATGTTT TTTTGCAGCT AAGTGAAGGA
    AACTTCGCTT GTGCTCAGTA TCTTTGGCTT AGACATCGGG CTGACTTTGA AAGCAAGTTT
    GACGTGAAAA TGCTGGAGAA TTTGCTGAAT TCAATATCTA CACAATTCCC CTTGGAAAAC
    CTTTGTTCCT GGTTTAAAAA TGAAGTGATT CCGTTTGTGA GGAGGATTGT GCCCGAAGGA
    CAAAACATTC TTGCAAAATG GCTAGAACAA GCATCAAGGA ACCTAGAGTT AACTGATAAG
    GCAAACTGGC CAGAAAATGG ACTTCAGCTG GCAGAGGTGT TCTTCACTGC AGAAAAAACA
    GACCGGTTTG GATTCGCATC TTCTTGGCAC TGGATTTCCT TGGATTATCA AAACACAGAG
    GAAGTCCGCC AGTTACGGAC ACTGGTGAGC AAGTTGCGGG AGCTGATCAT TCTGCATAGG
    AAATACAACT GCAAGCTGGC CCTCTCGGAC TTTGAGAAGG AAAATGCCAC CACTGTGGTG
    TTCCGCATGT TCGACAGGGT CTCGGCCCCA GAGCTGATCC CTTCGGTCTT AGAGAAGTCT
    GTGAGAGTTT ACATTCGCGA ACAGAATCTG CAAGAGGAGG AGCTGCTGTT GCTCTATATA
    GAGGATTTAC TGAAGAGGTG TAGCTCAAAG TCAATGACTC TCTTCGACAC AGCGTGGGAA
    GCAAAGGCCA TGGCCGTGAT AAGATGCTTG TCTGACACAG ATCTCATCTT TGATGCTGTG
    CTGAAGATAA TGTACAAGGC GGTGGTTCCC TGGAGTGCAG CCGTGGAGCA GCTGGTGAAA
    CAGCACCTGG AAATGGACCA CCCCAAAGTC AAGTTATTGC AGGAAAGCTA CAAACTGATG
    GAGATGAAGA AACTTTTGCG AGGGTATGGG ATAAGAGAGG TGAATCTCCT GAACAAGGAA
    ATAATGAGAG TGATCCGATA CATTCTAAAG CAGGACATCC CGTCTTCTTT AGAAGATGCT
    CTGAAGGTGG CCCAGGGGTA CAGGCTGTCT GATGACGAAA TCTACAGCCT GAGAATCATC
    GATCTGATTG ACAGGGAGCA GGGGGGAGAT TGCCTCCTGC TACTGAAGTC TCTGCCTGCC
    GCTGAAGCCG AGAAGACTGC AGAAAGGGTT ATCATCTGGG CTCGACTGGC ACTGCAGGAG
    GAGCCTGACG GCTCTGAGGA GGACAAGGCC TGGAGAATAT CTGTGGCGAA GACATCGGTG
    GATATTCTCA AGATCCTGTG TGATATTCGA AAAGACAATC TGCAGAAGAA GGATGAATCT
    GAAGAATTCC TGAAGCGATT TCAAATGGTT GCTAGTTTAC AGGAGAACTT TGAGGTCTTC
    CTTCCATTTG AAGACTACAG CAACACCGCC TTGGTAGCTG GCCTTCGAGA ACAGTATATT
    AAGGCACAGG AAGCTGCACA GGCTGAGCAC AAACACAGGG GACCCCCCGG GCCCACCCCG
    GCTAGGGGGA CACACCTGAG CATCAAGTCA AAGCTGCACC GACAGGCACT GGCCCTGCAG
    GTGTCTGAAC AGGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAGGA CGGGAAGGTG
    GTGGCCGCCC TGAGCAAATG CAGAGATTTA CTTAAGCATT ACTGCAATGC CGACACTGGG
    AGGTTACTGT TTGTGGTGTG TCAGAAGCTC TGTCAGATGT TGGCTGACGA TGTCCCAATG
    GTGGCCCCAG GAGGACTCAG CCTTCCTTCT GAGATCCATG ACCTCGCATG CCACGCTGTC
    ACCATCTGCA GTCCAGATTA TTTGCTGGAT GTTTTAGAAC TAAGTAAATA CACTTTAACC
    GCTGTGGAGC TTTGCAGACA ATGCCAGATG GATGACTGTG GAATGCTGAT GAAGGCCGCT
    CTTGGAACTC ACAAGGATCC CTATGAAGAG TGGTCCTTTA GTGACTTCTT CAGTGAAGAT
    GGAATTGTTC TCGAGTCCCA GGTGGTGCTG CCTGTCATCT ATGAATTGAT CTCATCAGTG
    ATGCCCCCTG CAGAAAGCAA AAGACATCCT TTGGATTCTA TAAGTTTGCC ATACTGCTCC
    ACTAGTGAAG GAGAAAACCG TATTCTCCCT CTTGTAAGCT CCATCTCTGC TCTGCTCCGG
    AGCCTCCAGG AATGTAGTCA GTGGGAGCTA GCCCTGAGGT TCGTGGTGGG CTCCTTTGGT
    ACCTGCCTGC AGCACTCTAT GTCAAACGTC ATGAGTATCA GCTTGAGTAA ACAGTTACTT
    GGGAAGAACA CATTGGCGAA CTCCAGGCAT ATCATTATGG AATTGAAAGA GAAGTCCATC
    ACATTTATCA GAGAAAATGC TACGACACTC CTGCATAAAG TGTTTAACTG TCGGGTGGTG
    GATCTGGACC TCGCCTTGGC TTACTGCACT CTCTTACCTC AAAAGGATGT ATTCGACAAC
    CTCTGGAAGT TCATAGATAA AGCGTGGCAG AATTACGACA AAATTCTGGC ATTATCTTTG
    GTGGGCTCTC AGCTGGCAAA TCTCTACCAG GATATTGAAA CAGGACTTTG GTTCCACGAG
    CTCAGTATCG ATGCCAAGTG GGGTATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAGCCT
    GCTTTTAGAC AAAATTTTCT CACCAAGAAA GACCTTATCA AAGCTCTTGT GAATAACATA
    GACATGGACA CCAGCCTCAT TCTGGAGTAT TGCAGCACAT TTCAGCTGGA CTCTGACGCT
    GCCCTTCGGC TGTTCATTGA AACTCTGCTC CGCAACACTA GCAGCCAGAG CCAGGGAGAT
    GCTGCCCCGG AGTCCACAAA GCATCAGCAT TCCAAACTCC TGGCGAAAGC CACTGAGCTG
    GTCCCTTTAC TGAAGAACAC AAAGGATCTG GTGATCAGTC TTAGTGAGAT CCTCTATAAG
    CTGGACCCAT ATGATTATGA AATGATTGAT GTTGTCCTGA AAGTTTTAGA ACAAGCTAAC
    GAAAAGATAA CCAGTGTGAA CATCAATCAG GCGCTGAATC TTCTGAGACA CTTGAAGTCA
    TACAGAAGGA TCTCCCCGCC AGTGGACCAC GAATACCAGT ACGCGCTGGA GCACATGATC
    ACTCTGCCAC CGGCTGCTCA CACTAGACTG CCCTTTCACC TAATACTGTT TGGCACAGCA
    CAGAACTTCT GGAAAATTCT CTCCTCAGAG CTCAGCGAAG AGTCGCTGCC AACCCTGCTC
    TTAATCGCGA AGCTGATGAA GTTCTCCCTA GATACTCTCT ATGTGTCTAC CGCCAAACAC
    TTGTTTGAAA AAAACCTGAA GCCAAAGCTC CTGAAGTCGG CACAAGCCAG GTCCTCCACC
    CTGATGAGCA AGGAAGTGGA TAAGCTCATG CAGACGCTGG AGTCGTACCT CCTGTCCATC
    GTCAACCCCG AGTGGGCTGT CGCCATTGCC ATCAGCCTCA CCCAGGAAGT CCCAGAAGGC
    CCCTTCAAGA TGTCTAGTTT GAAGTTCTGC CTGTATTTAG CTGAGAGATG GCTACAAAAC
    ATCCCACCTC AGGATGAGAC GTGTGAAAAG GCCAAAGCCT TGCAGAAGAA GCTCTGCCTC
    CAGGTCCGGC TCTCAGGAAC GGAAGCCGTG CTCATAGCCC ACAAGCTGAA CGATCAGGAA
    TATTTAAGAG TGATAGGGAA GCCAGCGCAC CTCATCGTGA GCCTCTACGA ACATCCCAGC
    ATAAGCGAAA GGCTTTGTAC CACATCTGGC AAAGATTATC CCGATATCCA TACAGCCGCT
    AAAGAAATAG CCGAAGTCAA TGAAGTTAAT TTGGAAAAAA TCTGGGACAT GTTGTTGGAG
    AAATGGCTGT GCCCCTCAAC GGTGCCCAGT GAGAAAGCAT CGGAGTTCTT TGAGCTGGAA
    GAGGATGAAG TGCTGCATAG AGTCGTGTAT CTCCTCCAGG CTCGTCCTGT TGATTATTGT
    TCGAGAATGC TGTTTGTATT TGCCACTTCA GCTACAAGTA CACTGGGCAT GCGCCAGCTA
    ACGTTTGCCC ACAAAGCACG AGCTCTGCAG TGTCTCCTCT ATTTGGCTGA CAAGGAAACA
    ATTGAATCTC TCTTCAAAAA GCCCATTAAA GAAATGAAAT CCTATCTGAA ATGTATAACT
    TTCCTGGCAT CATTTGAGAC ATTGAATATT CCCATCACAT ATGAATTATT CTGCAACAGT
    CCCAAAGAAG GCATGATTAA GGGCTTGTGG AAGAACCACA GCCACGAACC TATGAGTACA
    CAGGTTAGTC ATGCTTGCGA GCAAGAAGGC ACTGTCGTAC TTCCTTCCAA GGCCCACTCT
    AGAGGGAGAG GTCCCCTTCT CTCGTGCTTA GTTGCCTTTG GGAGACCGGT CACTGATGCC
    GTTGGAGCTG GGACAGGTAT GAGGCCAGCA GGCATCCTGG GTTTTTATTA TTATTATTAT
    TGTTATTGTT GTTATTGTTG TTATTATTGT TATTGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006530313.1
    CDS176..6274
    Translation

    Target ORF information:

    RefSeq Version XM_006530250.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus kinetochore associated 1 (Kntc1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006530250.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTGGAACA ATATTGAGCT GCTAACAAGC GATGACACTG GAAGTGGATG CCTGAGTGTT 
    GGCTCAAGAA AAGAAAACGT GACTGCACTG TACCAAGCCG ACTTGCTTGG GAAGATCTCC
    TCTGAGAAGA CCTCACTGAG TCCGAAGATC CAAGCATTCA GCTTAAGCCA TGGGTTTATA
    ATCGTTGCAG ACCAGTCAGT GATATTGCTT GACAGTATAT GCAGATCACT CCAGTTGTTT
    CTTATCTTCG ATACTGACGT GGATGTGGTC GGCCTGTGTC AAGAAGGAAA GTTTCTCTTT
    GTGGGGGAGA GAAGCGGCAA CTTCCACCTT ATCTACGTCA CATCGAAGCA AACGCTGTTC
    ACCAAGGCTT TTGTGGAGAA AGCTTTGGAC GAGAGTCAGC GAACGTACCG GAATCTCATT
    ATTGAGAAAG ATGGTTCAAA TGAAGGTACC TATTATATGT TGCTTCTAAC GAACAATGGA
    TTTTTTTATA TTACAAACCT CCAGCTTTCA CAAATTGAAC AGGCCATTGA GAACACGGAC
    TTGGATTCAG CCAAGAAGTT ACAAGGACAA TTCAAATGTA GTTTTATTTC TACTGAAAAT
    TACCACAGTT GTCTCAGTCT TGTGGCATCT CAATCTGGAA CATTTGCAAG TAAAACTTCT
    GTGATAATTG GGGGAACCGG TAGTTGTGCA TTCTCAAAAT GGGAACCCGA TTCTACCAAG
    AAAGAAATGT CTCTTAAGAA CTTTGTGGGC ACAGACATTA TTAAAGGTGC AAAGTCATTT
    CAGCTGATCG ACAACCTGCT TTTTGTGCTG GATACTGATA ATGTGCTGAG CTTGTGGGAT
    GCTTACACTC TCACTCCTGT GTGGAACTGG CCCTCTCTTC CCGTAGAGCA GTTTGTTCTC
    ACGACAGAAG CGGATTCTCC CTCATCTGTT ACCTGGCAAG GAATTACAAA TCTCAAATTA
    GTCACCCTGA CAGCAACAGC GAAGGAGAAG ATGAGAAGCC TCATCATTTA CTCTCTGCCT
    TCGATGGAAA CACTGTACTC TTTGGAAGTA TCTAGTGTTT CTTCTTTAGT TCAAACAGGA
    ATCAGCACGG ACACTATATA TCTTCTGGAA GGAATTCACA AAAATGATCC TAACCTATGT
    GAAGACTCTG TGTCTGACTT AGTACTTAGG TACCTCACAG AGGTGTTACC AGAAAACAGA
    CTGAGCCGGT TACTTCACAA GCACAGATTT GCCGAAGCCG AGAGCTTTGC CATTCAGTTC
    GGACTGGATG TTGAGCTTGT TTATAAGGTC AAATCAAATG ATATGTTGGA GAAGCTGGCT
    TTGATTTCCT CGGACAAGAG TGAGCAGTCC AAATGGCAGC AACTTGTAGA TGAAGCTAAA
    GAAAACCTAT GCAAAATCCA GGACGATGAC TTCGTGGTGA ATTTCTGCCT CAAGGCCCAA
    TGGGTAACCT ATGAAACCAC CCAGGAAATG CTCAGTTATG CCAAAACCAG GCTCATGAAG
    AAAGAAGACA GAGCTCTACC TGCCTCTTCT GATGCCTTCA TGGAGGTGCT AAAAGCTCAT
    GCAAAGTTAA CTACATTTTA TGGAGCATTT GGACCAGAAA AATTCAGTGG TAGCTCTTGG
    ATTGAGTTCC TGAATAACGA AGATGACCTC AGGGATGTTT TTTTGCAGCT AAGTGAAGGA
    AACTTCGCTT GTGCTCAGTA TCTTTGGCTT AGACATCGGG CTGACTTTGA AAGCAAGTTT
    GACGTGAAAA TGCTGGAGAA TTTGCTGAAT TCAATATCTA CACAATTCCC CTTGGAAAAC
    CTTTGTTCCT GGTTTAAAAA TGAAGTGATT CCGTTTGTGA GGAGGATTGT GCCCGAAGGA
    CAAAACATTC TTGCAAAATG GCTAGAACAA GCATCAAGGA ACCTAGAGTT AACTGATAAG
    GCAAACTGGC CAGAAAATGG ACTTCAGCTG GCAGAGGTGT TCTTCACTGC AGAAAAAACA
    GACCGGTTTG GATTCGCATC TTCTTGGCAC TGGATTTCCT TGGATTATCA AAACACAGAG
    GAAGTCCGCC AGTTACGGAC ACTGGTGAGC AAGTTGCGGG AGCTGATCAT TCTGCATAGG
    AAATACAACT GCAAGCTGGC CCTCTCGGAC TTTGAGAAGG AAAATGCCAC CACTGTGGTG
    TTCCGCATGT TCGACAGGGT CTCGGCCCCA GAGCTGATCC CTTCGGTCTT AGAGAAGTCT
    GTGAGAGTTT ACATTCGCGA ACAGAATCTG CAAGAGGAGG AGCTGCTGTT GCTCTATATA
    GAGGATTTAC TGAAGAGGTG TAGCTCAAAG TCAATGACTC TCTTCGACAC AGCGTGGGAA
    GCAAAGGCCA TGGCCGTGAT AAGATGCTTG TCTGACACAG ATCTCATCTT TGATGCTGTG
    CTGAAGATAA TGTACAAGGC GGTGGTTCCC TGGAGTGCAG CCGTGGAGCA GCTGGTGAAA
    CAGCACCTGG AAATGGACCA CCCCAAAGTC AAGTTATTGC AGGAAAGCTA CAAACTGATG
    GAGATGAAGA AACTTTTGCG AGGGTATGGG ATAAGAGAGG TGAATCTCCT GAACAAGGAA
    ATAATGAGAG TGATCCGATA CATTCTAAAG CAGGACATCC CGTCTTCTTT AGAAGATGCT
    CTGAAGGTGG CCCAGGGGTA CAGGCTGTCT GATGACGAAA TCTACAGCCT GAGAATCATC
    GATCTGATTG ACAGGGAGCA GGGGGGAGAT TGCCTCCTGC TACTGAAGTC TCTGCCTGCC
    GCTGAAGCCG AGAAGACTGC AGAAAGGGTT ATCATCTGGG CTCGACTGGC ACTGCAGGAG
    GAGCCTGACG GCTCTGAGGA GGACAAGGCC TGGAGAATAT CTGTGGCGAA GACATCGGTG
    GATATTCTCA AGATCCTGTG TGATATTCGA AAAGACAATC TGCAGAAGAA GGATGAATCT
    GAAGAATTCC TGAAGCGATT TCAAATGGTT GCTAGTTTAC AGGAGAACTT TGAGGTCTTC
    CTTCCATTTG AAGACTACAG CAACACCGCC TTGGTAGCTG GCCTTCGAGA ACAGTATATT
    AAGGCACAGG AAGCTGCACA GGCTGAGCAC AAACACAGGG GACCCCCCGG GCCCACCCCG
    GCTAGGGGGA CACACCTGAG CATCAAGTCA AAGCTGCACC GACAGGCACT GGCCCTGCAG
    GTGTCTGAAC AGGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAGGA CGGGAAGGTG
    GTGGCCGCCC TGAGCAAATG CAGAGATTTA CTTAAGCATT ACTGCAATGC CGACACTGGG
    AGGTTACTGT TTGTGGTGTG TCAGAAGCTC TGTCAGATGT TGGCTGACGA TGTCCCAATG
    GTGGCCCCAG GAGGACTCAG CCTTCCTTCT GAGATCCATG ACCTCGCATG CCACGCTGTC
    ACCATCTGCA GTCCAGATTA TTTGCTGGAT GTTTTAGAAC TAAGTAAATA CACTTTAACC
    GCTGTGGAGC TTTGCAGACA ATGCCAGATG GATGACTGTG GAATGCTGAT GAAGGCCGCT
    CTTGGAACTC ACAAGGATCC CTATGAAGAG TGGTCCTTTA GTGACTTCTT CAGTGAAGAT
    GGAATTGTTC TCGAGTCCCA GGTGGTGCTG CCTGTCATCT ATGAATTGAT CTCATCAGTG
    ATGCCCCCTG CAGAAAGCAA AAGACATCCT TTGGATTCTA TAAGTTTGCC ATACTGCTCC
    ACTAGTGAAG GAGAAAACCG TATTCTCCCT CTTGTAAGCT CCATCTCTGC TCTGCTCCGG
    AGCCTCCAGG AATGTAGTCA GTGGGAGCTA GCCCTGAGGT TCGTGGTGGG CTCCTTTGGT
    ACCTGCCTGC AGCACTCTAT GTCAAACGTC ATGAGTATCA GCTTGAGTAA ACAGTTACTT
    GGGAAGAACA CATTGGCGAA CTCCAGGCAT ATCATTATGG AATTGAAAGA GAAGTCCATC
    ACATTTATCA GAGAAAATGC TACGACACTC CTGCATAAAG TGTTTAACTG TCGGGTGGTG
    GATCTGGACC TCGCCTTGGC TTACTGCACT CTCTTACCTC AAAAGGATGT ATTCGACAAC
    CTCTGGAAGT TCATAGATAA AGCGTGGCAG AATTACGACA AAATTCTGGC ATTATCTTTG
    GTGGGCTCTC AGCTGGCAAA TCTCTACCAG GATATTGAAA CAGGACTTTG GTTCCACGAG
    CTCAGTATCG ATGCCAAGTG GGGTATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAGCCT
    GCTTTTAGAC AAAATTTTCT CACCAAGAAA GACCTTATCA AAGCTCTTGT GAATAACATA
    GACATGGACA CCAGCCTCAT TCTGGAGTAT TGCAGCACAT TTCAGCTGGA CTCTGACGCT
    GCCCTTCGGC TGTTCATTGA AACTCTGCTC CGCAACACTA GCAGCCAGAG CCAGGGAGAT
    GCTGCCCCGG AGTCCACAAA GCATCAGCAT TCCAAACTCC TGGCGAAAGC CACTGAGCTG
    GTCCCTTTAC TGAAGAACAC AAAGGATCTG GTGATCAGTC TTAGTGAGAT CCTCTATAAG
    CTGGACCCAT ATGATTATGA AATGATTGAT GTTGTCCTGA AAGTTTTAGA ACAAGCTAAC
    GAAAAGATAA CCAGTGTGAA CATCAATCAG GCGCTGAATC TTCTGAGACA CTTGAAGTCA
    TACAGAAGGA TCTCCCCGCC AGTGGACCAC GAATACCAGT ACGCGCTGGA GCACATGATC
    ACTCTGCCAC CGGCTGCTCA CACTAGACTG CCCTTTCACC TAATACTGTT TGGCACAGCA
    CAGAACTTCT GGAAAATTCT CTCCTCAGAG CTCAGCGAAG AGTCGCTGCC AACCCTGCTC
    TTAATCGCGA AGCTGATGAA GTTCTCCCTA GATACTCTCT ATGTGTCTAC CGCCAAACAC
    TTGTTTGAAA AAAACCTGAA GCCAAAGCTC CTGAAGTCGG CACAAGCCAG GTCCTCCACC
    CTGATGAGCA AGGAAGTGGA TAAGCTCATG CAGACGCTGG AGTCGTACCT CCTGTCCATC
    GTCAACCCCG AGTGGGCTGT CGCCATTGCC ATCAGCCTCA CCCAGGAAGT CCCAGAAGGC
    CCCTTCAAGA TGTCTAGTTT GAAGTTCTGC CTGTATTTAG CTGAGAGATG GCTACAAAAC
    ATCCCACCTC AGGATGAGAC GTGTGAAAAG GCCAAAGCCT TGCAGAAGAA GCTCTGCCTC
    CAGGTCCGGC TCTCAGGAAC GGAAGCCGTG CTCATAGCCC ACAAGCTGAA CGATCAGGAA
    TATTTAAGAG TGATAGGGAA GCCAGCGCAC CTCATCGTGA GCCTCTACGA ACATCCCAGC
    ATAAGCGAAA GGCTTTGTAC CACATCTGGC AAAGATTATC CCGATATCCA TACAGCCGCT
    AAAGAAATAG CCGAAGTCAA TGAAGTTAAT TTGGAAAAAA TCTGGGACAT GTTGTTGGAG
    AAATGGCTGT GCCCCTCAAC GGTGCCCAGT GAGAAAGCAT CGGAGTTCTT TGAGCTGGAA
    GAGGATGAAG TGCTGCATAG AGTCGTGTAT CTCCTCCAGG CTCGTCCTGT TGATTATTGT
    TCGAGAATGC TGTTTGTATT TGCCACTTCA GCTACAAGTA CACTGGGCAT GCGCCAGCTA
    ACGTTTGCCC ACAAAGCACG AGCTCTGCAG TGTCTCCTCT ATTTGGCTGA CAAGGAAACA
    ATTGAATCTC TCTTCAAAAA GCCCATTAAA GAAATGAAAT CCTATCTGAA ATGTATAACT
    TTCCTGGCAT CATTTGAGAC ATTGAATATT CCCATCACAT ATGAATTATT CTGCAACAGT
    CCCAAAGAAG GCATGATTAA GGGCTTGTGG AAGAACCACA GCCACGAACC TATGAGTACA
    CAGGTTAGTC ATGCTTGCGA GCAAGAAGGC ACTGTCGTAC TTCCTTCCAA GGCCCACTCT
    AGAGGGAGAG GTCCCCTTCT CTCGTGCTTA GTTGCCTTTG GGAGACCGGT CACTGATGCC
    GTTGGAGCTG GGACAGGTAT GAGGCCAGCA GGCATCCTGG GTTTTTATTA TTATTATTAT
    TGTTATTGTT GTTATTGTTG TTATTATTGT TATTGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu00882
    Clone ID Related Accession (Same CDS sequence) NM_001042421.1
    Accession Version NM_001042421.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6624bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-19
    Organism Mus musculus(house mouse)
    Product kinetochore-associated protein 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY010626.1, AK172905.1 and AC129582.10. On or before Jun 21, 2006 this sequence version replaced XM_916900.2, XM_924555.2, XM_924553.2, XM_977852.1, XM_001000208.1. ##Evidence-Data-START## Transcript exon combination :: AK172905.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTGGAACA ATATTGAGCT GCTAACAAGC GATGACACTG GAAGTGGATG CCTGAGTGTT 
    GGCTCAAGAA AAGAAAACGT GACTGCACTG TACCAAGCCG ACTTGCTTGG GAAGATCTCC
    TCTGAGAAGA CCTCACTGAG TCCGAAGATC CAAGCATTCA GCTTAAGCCA TGGGTTTATA
    ATCGTTGCAG ACCAGTCAGT GATATTGCTT GACAGTATAT GCAGATCACT CCAGTTGTTT
    CTTATCTTCG ATACTGACGT GGATGTGGTC GGCCTGTGTC AAGAAGGAAA GTTTCTCTTT
    GTGGGGGAGA GAAGCGGCAA CTTCCACCTT ATCTACGTCA CATCGAAGCA AACGCTGTTC
    ACCAAGGCTT TTGTGGAGAA AGCTTTGGAC GAGAGTCAGC GAACGTACCG GAATCTCATT
    ATTGAGAAAG ATGGTTCAAA TGAAGGTACC TATTATATGT TGCTTCTAAC GAACAATGGA
    TTTTTTTATA TTACAAACCT CCAGCTTTCA CAAATTGAAC AGGCCATTGA GAACACGGAC
    TTGGATTCAG CCAAGAAGTT ACAAGGACAA TTCAAATGTA GTTTTATTTC TACTGAAAAT
    TACCACAGTT GTCTCAGTCT TGTGGCATCT CAATCTGGAA CATTTGCAAG TAAAACTTCT
    GTGATAATTG GGGGAACCGG TAGTTGTGCA TTCTCAAAAT GGGAACCCGA TTCTACCAAG
    AAAGAAATGT CTCTTAAGAA CTTTGTGGGC ACAGACATTA TTAAAGGTGC AAAGTCATTT
    CAGCTGATCG ACAACCTGCT TTTTGTGCTG GATACTGATA ATGTGCTGAG CTTGTGGGAT
    GCTTACACTC TCACTCCTGT GTGGAACTGG CCCTCTCTTC CCGTAGAGCA GTTTGTTCTC
    ACGACAGAAG CGGATTCTCC CTCATCTGTT ACCTGGCAAG GAATTACAAA TCTCAAATTA
    GTCACCCTGA CAGCAACAGC GAAGGAGAAG ATGAGAAGCC TCATCATTTA CTCTCTGCCT
    TCGATGGAAA CACTGTACTC TTTGGAAGTA TCTAGTGTTT CTTCTTTAGT TCAAACAGGA
    ATCAGCACGG ACACTATATA TCTTCTGGAA GGAATTCACA AAAATGATCC TAACCTATGT
    GAAGACTCTG TGTCTGACTT AGTACTTAGG TACCTCACAG AGGTGTTACC AGAAAACAGA
    CTGAGCCGGT TACTTCACAA GCACAGATTT GCCGAAGCCG AGAGCTTTGC CATTCAGTTC
    GGACTGGATG TTGAGCTTGT TTATAAGGTC AAATCAAATG ATATGTTGGA GAAGCTGGCT
    TTGATTTCCT CGGACAAGAG TGAGCAGTCC AAATGGCAGC AACTTGTAGA TGAAGCTAAA
    GAAAACCTAT GCAAAATCCA GGACGATGAC TTCGTGGTGA ATTTCTGCCT CAAGGCCCAA
    TGGGTAACCT ATGAAACCAC CCAGGAAATG CTCAGTTATG CCAAAACCAG GCTCATGAAG
    AAAGAAGACA GAGCTCTACC TGCCTCTTCT GATGCCTTCA TGGAGGTGCT AAAAGCTCAT
    GCAAAGTTAA CTACATTTTA TGGAGCATTT GGACCAGAAA AATTCAGTGG TAGCTCTTGG
    ATTGAGTTCC TGAATAACGA AGATGACCTC AGGGATGTTT TTTTGCAGCT AAGTGAAGGA
    AACTTCGCTT GTGCTCAGTA TCTTTGGCTT AGACATCGGG CTGACTTTGA AAGCAAGTTT
    GACGTGAAAA TGCTGGAGAA TTTGCTGAAT TCAATATCTA CACAATTCCC CTTGGAAAAC
    CTTTGTTCCT GGTTTAAAAA TGAAGTGATT CCGTTTGTGA GGAGGATTGT GCCCGAAGGA
    CAAAACATTC TTGCAAAATG GCTAGAACAA GCATCAAGGA ACCTAGAGTT AACTGATAAG
    GCAAACTGGC CAGAAAATGG ACTTCAGCTG GCAGAGGTGT TCTTCACTGC AGAAAAAACA
    GACCGGTTTG GATTCGCATC TTCTTGGCAC TGGATTTCCT TGGATTATCA AAACACAGAG
    GAAGTCCGCC AGTTACGGAC ACTGGTGAGC AAGTTGCGGG AGCTGATCAT TCTGCATAGG
    AAATACAACT GCAAGCTGGC CCTCTCGGAC TTTGAGAAGG AAAATGCCAC CACTGTGGTG
    TTCCGCATGT TCGACAGGGT CTCGGCCCCA GAGCTGATCC CTTCGGTCTT AGAGAAGTCT
    GTGAGAGTTT ACATTCGCGA ACAGAATCTG CAAGAGGAGG AGCTGCTGTT GCTCTATATA
    GAGGATTTAC TGAAGAGGTG TAGCTCAAAG TCAATGACTC TCTTCGACAC AGCGTGGGAA
    GCAAAGGCCA TGGCCGTGAT AAGATGCTTG TCTGACACAG ATCTCATCTT TGATGCTGTG
    CTGAAGATAA TGTACAAGGC GGTGGTTCCC TGGAGTGCAG CCGTGGAGCA GCTGGTGAAA
    CAGCACCTGG AAATGGACCA CCCCAAAGTC AAGTTATTGC AGGAAAGCTA CAAACTGATG
    GAGATGAAGA AACTTTTGCG AGGGTATGGG ATAAGAGAGG TGAATCTCCT GAACAAGGAA
    ATAATGAGAG TGATCCGATA CATTCTAAAG CAGGACATCC CGTCTTCTTT AGAAGATGCT
    CTGAAGGTGG CCCAGGGGTA CAGGCTGTCT GATGACGAAA TCTACAGCCT GAGAATCATC
    GATCTGATTG ACAGGGAGCA GGGGGGAGAT TGCCTCCTGC TACTGAAGTC TCTGCCTGCC
    GCTGAAGCCG AGAAGACTGC AGAAAGGGTT ATCATCTGGG CTCGACTGGC ACTGCAGGAG
    GAGCCTGACG GCTCTGAGGA GGACAAGGCC TGGAGAATAT CTGTGGCGAA GACATCGGTG
    GATATTCTCA AGATCCTGTG TGATATTCGA AAAGACAATC TGCAGAAGAA GGATGAATCT
    GAAGAATTCC TGAAGCGATT TCAAATGGTT GCTAGTTTAC AGGAGAACTT TGAGGTCTTC
    CTTCCATTTG AAGACTACAG CAACACCGCC TTGGTAGCTG GCCTTCGAGA ACAGTATATT
    AAGGCACAGG AAGCTGCACA GGCTGAGCAC AAACACAGGG GACCCCCCGG GCCCACCCCG
    GCTAGGGGGA CACACCTGAG CATCAAGTCA AAGCTGCACC GACAGGCACT GGCCCTGCAG
    GTGTCTGAAC AGGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAGGA CGGGAAGGTG
    GTGGCCGCCC TGAGCAAATG CAGAGATTTA CTTAAGCATT ACTGCAATGC CGACACTGGG
    AGGTTACTGT TTGTGGTGTG TCAGAAGCTC TGTCAGATGT TGGCTGACGA TGTCCCAATG
    GTGGCCCCAG GAGGACTCAG CCTTCCTTCT GAGATCCATG ACCTCGCATG CCACGCTGTC
    ACCATCTGCA GTCCAGATTA TTTGCTGGAT GTTTTAGAAC TAAGTAAATA CACTTTAACC
    GCTGTGGAGC TTTGCAGACA ATGCCAGATG GATGACTGTG GAATGCTGAT GAAGGCCGCT
    CTTGGAACTC ACAAGGATCC CTATGAAGAG TGGTCCTTTA GTGACTTCTT CAGTGAAGAT
    GGAATTGTTC TCGAGTCCCA GGTGGTGCTG CCTGTCATCT ATGAATTGAT CTCATCAGTG
    ATGCCCCCTG CAGAAAGCAA AAGACATCCT TTGGATTCTA TAAGTTTGCC ATACTGCTCC
    ACTAGTGAAG GAGAAAACCG TATTCTCCCT CTTGTAAGCT CCATCTCTGC TCTGCTCCGG
    AGCCTCCAGG AATGTAGTCA GTGGGAGCTA GCCCTGAGGT TCGTGGTGGG CTCCTTTGGT
    ACCTGCCTGC AGCACTCTAT GTCAAACGTC ATGAGTATCA GCTTGAGTAA ACAGTTACTT
    GGGAAGAACA CATTGGCGAA CTCCAGGCAT ATCATTATGG AATTGAAAGA GAAGTCCATC
    ACATTTATCA GAGAAAATGC TACGACACTC CTGCATAAAG TGTTTAACTG TCGGGTGGTG
    GATCTGGACC TCGCCTTGGC TTACTGCACT CTCTTACCTC AAAAGGATGT ATTCGACAAC
    CTCTGGAAGT TCATAGATAA AGCGTGGCAG AATTACGACA AAATTCTGGC ATTATCTTTG
    GTGGGCTCTC AGCTGGCAAA TCTCTACCAG GATATTGAAA CAGGACTTTG GTTCCACGAG
    CTCAGTATCG ATGCCAAGTG GGGTATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAGCCT
    GCTTTTAGAC AAAATTTTCT CACCAAGAAA GACCTTATCA AAGCTCTTGT GAATAACATA
    GACATGGACA CCAGCCTCAT TCTGGAGTAT TGCAGCACAT TTCAGCTGGA CTCTGACGCT
    GCCCTTCGGC TGTTCATTGA AACTCTGCTC CGCAACACTA GCAGCCAGAG CCAGGGAGAT
    GCTGCCCCGG AGTCCACAAA GCATCAGCAT TCCAAACTCC TGGCGAAAGC CACTGAGCTG
    GTCCCTTTAC TGAAGAACAC AAAGGATCTG GTGATCAGTC TTAGTGAGAT CCTCTATAAG
    CTGGACCCAT ATGATTATGA AATGATTGAT GTTGTCCTGA AAGTTTTAGA ACAAGCTAAC
    GAAAAGATAA CCAGTGTGAA CATCAATCAG GCGCTGAATC TTCTGAGACA CTTGAAGTCA
    TACAGAAGGA TCTCCCCGCC AGTGGACCAC GAATACCAGT ACGCGCTGGA GCACATGATC
    ACTCTGCCAC CGGCTGCTCA CACTAGACTG CCCTTTCACC TAATACTGTT TGGCACAGCA
    CAGAACTTCT GGAAAATTCT CTCCTCAGAG CTCAGCGAAG AGTCGCTGCC AACCCTGCTC
    TTAATCGCGA AGCTGATGAA GTTCTCCCTA GATACTCTCT ATGTGTCTAC CGCCAAACAC
    TTGTTTGAAA AAAACCTGAA GCCAAAGCTC CTGAAGTCGG CACAAGCCAG GTCCTCCACC
    CTGATGAGCA AGGAAGTGGA TAAGCTCATG CAGACGCTGG AGTCGTACCT CCTGTCCATC
    GTCAACCCCG AGTGGGCTGT CGCCATTGCC ATCAGCCTCA CCCAGGAAGT CCCAGAAGGC
    CCCTTCAAGA TGTCTAGTTT GAAGTTCTGC CTGTATTTAG CTGAGAGATG GCTACAAAAC
    ATCCCACCTC AGGATGAGAC GTGTGAAAAG GCCAAAGCCT TGCAGAAGAA GCTCTGCCTC
    CAGGTCCGGC TCTCAGGAAC GGAAGCCGTG CTCATAGCCC ACAAGCTGAA CGATCAGGAA
    TATTTAAGAG TGATAGGGAA GCCAGCGCAC CTCATCGTGA GCCTCTACGA ACATCCCAGC
    ATAAGCGAAA GGCTTTGTAC CACATCTGGC AAAGATTATC CCGATATCCA TACAGCCGCT
    AAAGAAATAG CCGAAGTCAA TGAAGTTAAT TTGGAAAAAA TCTGGGACAT GTTGTTGGAG
    AAATGGCTGT GCCCCTCAAC GGTGCCCAGT GAGAAAGCAT CGGAGTTCTT TGAGCTGGAA
    GAGGATGAAG TGCTGCATAG AGTCGTGTAT CTCCTCCAGG CTCGTCCTGT TGATTATTGT
    TCGAGAATGC TGTTTGTATT TGCCACTTCA GCTACAAGTA CACTGGGCAT GCGCCAGCTA
    ACGTTTGCCC ACAAAGCACG AGCTCTGCAG TGTCTCCTCT ATTTGGCTGA CAAGGAAACA
    ATTGAATCTC TCTTCAAAAA GCCCATTAAA GAAATGAAAT CCTATCTGAA ATGTATAACT
    TTCCTGGCAT CATTTGAGAC ATTGAATATT CCCATCACAT ATGAATTATT CTGCAACAGT
    CCCAAAGAAG GCATGATTAA GGGCTTGTGG AAGAACCACA GCCACGAACC TATGGCAGTA
    CGATTGGTTG CAGAGCTTTG CTTAGAATAC AAAATCTATG ACCTGCAGCT TTGGAATGGG
    CTCTTGCAGA AGCTTCTGGG CTTCAACATG ATTCCTTACC TGAGGAAAGT GTTATCCTGC
    ATTTCTAGCA TCCATTCGTT ATGGCAGGTG CCCTACTTCA GCAAAGCGTG GCAGCGCGTG
    ATCCAGATCC CTCTGCTCTC GGCTTCCTGT CCTTTAAGAC CCAGTCAGTT AGCAGACTGC
    TGTGACAGTC TCGTCGCCAT CCTTGAATGT CCAGTTTCAG ATGACCTGGA CATGATGGGA
    GTCGCCAAGC AGTACGTCCA GTTAGACCTT CCCGCTTTTG CTCTGACCTG CCTGACGCTC
    ATGCCCCACT CTGAAAAAAG ACACCAGCAG ATCAAGAATT TTCTGAATTC CTGTGATGCT
    CGGATCATTT TACAGCAGAT AGAAGAACAT ATGAACACTG GCCAGCTAGC AGGGTTTTCA
    CATCAGATTG GAAGTCTGGT TCTGAATCAT GTTGTAAATA AGAAGGAATT TGGGATTTTG
    GCAAAGACAA AGTATTTTCA GTTGTTGAAA TGCCACGTGA TAAACACCGG CAACGTCACT
    GAATTGGTAA ACTATTTGGC AAATGACTTC AGTGTAGATG AAGCATCAGC GTTGATAAAT
    GAGTATTCAA AGCATTGTGG GAAGCCTGTG CCAGCAGATG CAGCTCCCTG TGAGATCCTG
    CAGACATTTC TTGGTGGATC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001035886.1
    CDS146..6769
    Translation

    Target ORF information:

    RefSeq Version NM_001042421.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus kinetochore associated 1 (Kntc1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001042421.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTGGAACA ATATTGAGCT GCTAACAAGC GATGACACTG GAAGTGGATG CCTGAGTGTT 
    GGCTCAAGAA AAGAAAACGT GACTGCACTG TACCAAGCCG ACTTGCTTGG GAAGATCTCC
    TCTGAGAAGA CCTCACTGAG TCCGAAGATC CAAGCATTCA GCTTAAGCCA TGGGTTTATA
    ATCGTTGCAG ACCAGTCAGT GATATTGCTT GACAGTATAT GCAGATCACT CCAGTTGTTT
    CTTATCTTCG ATACTGACGT GGATGTGGTC GGCCTGTGTC AAGAAGGAAA GTTTCTCTTT
    GTGGGGGAGA GAAGCGGCAA CTTCCACCTT ATCTACGTCA CATCGAAGCA AACGCTGTTC
    ACCAAGGCTT TTGTGGAGAA AGCTTTGGAC GAGAGTCAGC GAACGTACCG GAATCTCATT
    ATTGAGAAAG ATGGTTCAAA TGAAGGTACC TATTATATGT TGCTTCTAAC GAACAATGGA
    TTTTTTTATA TTACAAACCT CCAGCTTTCA CAAATTGAAC AGGCCATTGA GAACACGGAC
    TTGGATTCAG CCAAGAAGTT ACAAGGACAA TTCAAATGTA GTTTTATTTC TACTGAAAAT
    TACCACAGTT GTCTCAGTCT TGTGGCATCT CAATCTGGAA CATTTGCAAG TAAAACTTCT
    GTGATAATTG GGGGAACCGG TAGTTGTGCA TTCTCAAAAT GGGAACCCGA TTCTACCAAG
    AAAGAAATGT CTCTTAAGAA CTTTGTGGGC ACAGACATTA TTAAAGGTGC AAAGTCATTT
    CAGCTGATCG ACAACCTGCT TTTTGTGCTG GATACTGATA ATGTGCTGAG CTTGTGGGAT
    GCTTACACTC TCACTCCTGT GTGGAACTGG CCCTCTCTTC CCGTAGAGCA GTTTGTTCTC
    ACGACAGAAG CGGATTCTCC CTCATCTGTT ACCTGGCAAG GAATTACAAA TCTCAAATTA
    GTCACCCTGA CAGCAACAGC GAAGGAGAAG ATGAGAAGCC TCATCATTTA CTCTCTGCCT
    TCGATGGAAA CACTGTACTC TTTGGAAGTA TCTAGTGTTT CTTCTTTAGT TCAAACAGGA
    ATCAGCACGG ACACTATATA TCTTCTGGAA GGAATTCACA AAAATGATCC TAACCTATGT
    GAAGACTCTG TGTCTGACTT AGTACTTAGG TACCTCACAG AGGTGTTACC AGAAAACAGA
    CTGAGCCGGT TACTTCACAA GCACAGATTT GCCGAAGCCG AGAGCTTTGC CATTCAGTTC
    GGACTGGATG TTGAGCTTGT TTATAAGGTC AAATCAAATG ATATGTTGGA GAAGCTGGCT
    TTGATTTCCT CGGACAAGAG TGAGCAGTCC AAATGGCAGC AACTTGTAGA TGAAGCTAAA
    GAAAACCTAT GCAAAATCCA GGACGATGAC TTCGTGGTGA ATTTCTGCCT CAAGGCCCAA
    TGGGTAACCT ATGAAACCAC CCAGGAAATG CTCAGTTATG CCAAAACCAG GCTCATGAAG
    AAAGAAGACA GAGCTCTACC TGCCTCTTCT GATGCCTTCA TGGAGGTGCT AAAAGCTCAT
    GCAAAGTTAA CTACATTTTA TGGAGCATTT GGACCAGAAA AATTCAGTGG TAGCTCTTGG
    ATTGAGTTCC TGAATAACGA AGATGACCTC AGGGATGTTT TTTTGCAGCT AAGTGAAGGA
    AACTTCGCTT GTGCTCAGTA TCTTTGGCTT AGACATCGGG CTGACTTTGA AAGCAAGTTT
    GACGTGAAAA TGCTGGAGAA TTTGCTGAAT TCAATATCTA CACAATTCCC CTTGGAAAAC
    CTTTGTTCCT GGTTTAAAAA TGAAGTGATT CCGTTTGTGA GGAGGATTGT GCCCGAAGGA
    CAAAACATTC TTGCAAAATG GCTAGAACAA GCATCAAGGA ACCTAGAGTT AACTGATAAG
    GCAAACTGGC CAGAAAATGG ACTTCAGCTG GCAGAGGTGT TCTTCACTGC AGAAAAAACA
    GACCGGTTTG GATTCGCATC TTCTTGGCAC TGGATTTCCT TGGATTATCA AAACACAGAG
    GAAGTCCGCC AGTTACGGAC ACTGGTGAGC AAGTTGCGGG AGCTGATCAT TCTGCATAGG
    AAATACAACT GCAAGCTGGC CCTCTCGGAC TTTGAGAAGG AAAATGCCAC CACTGTGGTG
    TTCCGCATGT TCGACAGGGT CTCGGCCCCA GAGCTGATCC CTTCGGTCTT AGAGAAGTCT
    GTGAGAGTTT ACATTCGCGA ACAGAATCTG CAAGAGGAGG AGCTGCTGTT GCTCTATATA
    GAGGATTTAC TGAAGAGGTG TAGCTCAAAG TCAATGACTC TCTTCGACAC AGCGTGGGAA
    GCAAAGGCCA TGGCCGTGAT AAGATGCTTG TCTGACACAG ATCTCATCTT TGATGCTGTG
    CTGAAGATAA TGTACAAGGC GGTGGTTCCC TGGAGTGCAG CCGTGGAGCA GCTGGTGAAA
    CAGCACCTGG AAATGGACCA CCCCAAAGTC AAGTTATTGC AGGAAAGCTA CAAACTGATG
    GAGATGAAGA AACTTTTGCG AGGGTATGGG ATAAGAGAGG TGAATCTCCT GAACAAGGAA
    ATAATGAGAG TGATCCGATA CATTCTAAAG CAGGACATCC CGTCTTCTTT AGAAGATGCT
    CTGAAGGTGG CCCAGGGGTA CAGGCTGTCT GATGACGAAA TCTACAGCCT GAGAATCATC
    GATCTGATTG ACAGGGAGCA GGGGGGAGAT TGCCTCCTGC TACTGAAGTC TCTGCCTGCC
    GCTGAAGCCG AGAAGACTGC AGAAAGGGTT ATCATCTGGG CTCGACTGGC ACTGCAGGAG
    GAGCCTGACG GCTCTGAGGA GGACAAGGCC TGGAGAATAT CTGTGGCGAA GACATCGGTG
    GATATTCTCA AGATCCTGTG TGATATTCGA AAAGACAATC TGCAGAAGAA GGATGAATCT
    GAAGAATTCC TGAAGCGATT TCAAATGGTT GCTAGTTTAC AGGAGAACTT TGAGGTCTTC
    CTTCCATTTG AAGACTACAG CAACACCGCC TTGGTAGCTG GCCTTCGAGA ACAGTATATT
    AAGGCACAGG AAGCTGCACA GGCTGAGCAC AAACACAGGG GACCCCCCGG GCCCACCCCG
    GCTAGGGGGA CACACCTGAG CATCAAGTCA AAGCTGCACC GACAGGCACT GGCCCTGCAG
    GTGTCTGAAC AGGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAGGA CGGGAAGGTG
    GTGGCCGCCC TGAGCAAATG CAGAGATTTA CTTAAGCATT ACTGCAATGC CGACACTGGG
    AGGTTACTGT TTGTGGTGTG TCAGAAGCTC TGTCAGATGT TGGCTGACGA TGTCCCAATG
    GTGGCCCCAG GAGGACTCAG CCTTCCTTCT GAGATCCATG ACCTCGCATG CCACGCTGTC
    ACCATCTGCA GTCCAGATTA TTTGCTGGAT GTTTTAGAAC TAAGTAAATA CACTTTAACC
    GCTGTGGAGC TTTGCAGACA ATGCCAGATG GATGACTGTG GAATGCTGAT GAAGGCCGCT
    CTTGGAACTC ACAAGGATCC CTATGAAGAG TGGTCCTTTA GTGACTTCTT CAGTGAAGAT
    GGAATTGTTC TCGAGTCCCA GGTGGTGCTG CCTGTCATCT ATGAATTGAT CTCATCAGTG
    ATGCCCCCTG CAGAAAGCAA AAGACATCCT TTGGATTCTA TAAGTTTGCC ATACTGCTCC
    ACTAGTGAAG GAGAAAACCG TATTCTCCCT CTTGTAAGCT CCATCTCTGC TCTGCTCCGG
    AGCCTCCAGG AATGTAGTCA GTGGGAGCTA GCCCTGAGGT TCGTGGTGGG CTCCTTTGGT
    ACCTGCCTGC AGCACTCTAT GTCAAACGTC ATGAGTATCA GCTTGAGTAA ACAGTTACTT
    GGGAAGAACA CATTGGCGAA CTCCAGGCAT ATCATTATGG AATTGAAAGA GAAGTCCATC
    ACATTTATCA GAGAAAATGC TACGACACTC CTGCATAAAG TGTTTAACTG TCGGGTGGTG
    GATCTGGACC TCGCCTTGGC TTACTGCACT CTCTTACCTC AAAAGGATGT ATTCGACAAC
    CTCTGGAAGT TCATAGATAA AGCGTGGCAG AATTACGACA AAATTCTGGC ATTATCTTTG
    GTGGGCTCTC AGCTGGCAAA TCTCTACCAG GATATTGAAA CAGGACTTTG GTTCCACGAG
    CTCAGTATCG ATGCCAAGTG GGGTATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAGCCT
    GCTTTTAGAC AAAATTTTCT CACCAAGAAA GACCTTATCA AAGCTCTTGT GAATAACATA
    GACATGGACA CCAGCCTCAT TCTGGAGTAT TGCAGCACAT TTCAGCTGGA CTCTGACGCT
    GCCCTTCGGC TGTTCATTGA AACTCTGCTC CGCAACACTA GCAGCCAGAG CCAGGGAGAT
    GCTGCCCCGG AGTCCACAAA GCATCAGCAT TCCAAACTCC TGGCGAAAGC CACTGAGCTG
    GTCCCTTTAC TGAAGAACAC AAAGGATCTG GTGATCAGTC TTAGTGAGAT CCTCTATAAG
    CTGGACCCAT ATGATTATGA AATGATTGAT GTTGTCCTGA AAGTTTTAGA ACAAGCTAAC
    GAAAAGATAA CCAGTGTGAA CATCAATCAG GCGCTGAATC TTCTGAGACA CTTGAAGTCA
    TACAGAAGGA TCTCCCCGCC AGTGGACCAC GAATACCAGT ACGCGCTGGA GCACATGATC
    ACTCTGCCAC CGGCTGCTCA CACTAGACTG CCCTTTCACC TAATACTGTT TGGCACAGCA
    CAGAACTTCT GGAAAATTCT CTCCTCAGAG CTCAGCGAAG AGTCGCTGCC AACCCTGCTC
    TTAATCGCGA AGCTGATGAA GTTCTCCCTA GATACTCTCT ATGTGTCTAC CGCCAAACAC
    TTGTTTGAAA AAAACCTGAA GCCAAAGCTC CTGAAGTCGG CACAAGCCAG GTCCTCCACC
    CTGATGAGCA AGGAAGTGGA TAAGCTCATG CAGACGCTGG AGTCGTACCT CCTGTCCATC
    GTCAACCCCG AGTGGGCTGT CGCCATTGCC ATCAGCCTCA CCCAGGAAGT CCCAGAAGGC
    CCCTTCAAGA TGTCTAGTTT GAAGTTCTGC CTGTATTTAG CTGAGAGATG GCTACAAAAC
    ATCCCACCTC AGGATGAGAC GTGTGAAAAG GCCAAAGCCT TGCAGAAGAA GCTCTGCCTC
    CAGGTCCGGC TCTCAGGAAC GGAAGCCGTG CTCATAGCCC ACAAGCTGAA CGATCAGGAA
    TATTTAAGAG TGATAGGGAA GCCAGCGCAC CTCATCGTGA GCCTCTACGA ACATCCCAGC
    ATAAGCGAAA GGCTTTGTAC CACATCTGGC AAAGATTATC CCGATATCCA TACAGCCGCT
    AAAGAAATAG CCGAAGTCAA TGAAGTTAAT TTGGAAAAAA TCTGGGACAT GTTGTTGGAG
    AAATGGCTGT GCCCCTCAAC GGTGCCCAGT GAGAAAGCAT CGGAGTTCTT TGAGCTGGAA
    GAGGATGAAG TGCTGCATAG AGTCGTGTAT CTCCTCCAGG CTCGTCCTGT TGATTATTGT
    TCGAGAATGC TGTTTGTATT TGCCACTTCA GCTACAAGTA CACTGGGCAT GCGCCAGCTA
    ACGTTTGCCC ACAAAGCACG AGCTCTGCAG TGTCTCCTCT ATTTGGCTGA CAAGGAAACA
    ATTGAATCTC TCTTCAAAAA GCCCATTAAA GAAATGAAAT CCTATCTGAA ATGTATAACT
    TTCCTGGCAT CATTTGAGAC ATTGAATATT CCCATCACAT ATGAATTATT CTGCAACAGT
    CCCAAAGAAG GCATGATTAA GGGCTTGTGG AAGAACCACA GCCACGAACC TATGGCAGTA
    CGATTGGTTG CAGAGCTTTG CTTAGAATAC AAAATCTATG ACCTGCAGCT TTGGAATGGG
    CTCTTGCAGA AGCTTCTGGG CTTCAACATG ATTCCTTACC TGAGGAAAGT GTTATCCTGC
    ATTTCTAGCA TCCATTCGTT ATGGCAGGTG CCCTACTTCA GCAAAGCGTG GCAGCGCGTG
    ATCCAGATCC CTCTGCTCTC GGCTTCCTGT CCTTTAAGAC CCAGTCAGTT AGCAGACTGC
    TGTGACAGTC TCGTCGCCAT CCTTGAATGT CCAGTTTCAG ATGACCTGGA CATGATGGGA
    GTCGCCAAGC AGTACGTCCA GTTAGACCTT CCCGCTTTTG CTCTGACCTG CCTGACGCTC
    ATGCCCCACT CTGAAAAAAG ACACCAGCAG ATCAAGAATT TTCTGAATTC CTGTGATGCT
    CGGATCATTT TACAGCAGAT AGAAGAACAT ATGAACACTG GCCAGCTAGC AGGGTTTTCA
    CATCAGATTG GAAGTCTGGT TCTGAATCAT GTTGTAAATA AGAAGGAATT TGGGATTTTG
    GCAAAGACAA AGTATTTTCA GTTGTTGAAA TGCCACGTGA TAAACACCGG CAACGTCACT
    GAATTGGTAA ACTATTTGGC AAATGACTTC AGTGTAGATG AAGCATCAGC GTTGATAAAT
    GAGTATTCAA AGCATTGTGG GAAGCCTGTG CCAGCAGATG CAGCTCCCTG TGAGATCCTG
    CAGACATTTC TTGGTGGATC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35844
    Clone ID Related Accession (Same CDS sequence) XM_006530249.1 , XM_030254330.1
    Accession Version XM_006530249.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6168bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product kinetochore-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTTGCTTC TAACGAACAA TGGATTTTTT TATATTACAA ACCTCCAGCT TTCACAAATT 
    GAACAGGCCA TTGAGAACAC GGACTTGGAT TCAGCCAAGA AGTTACAAGG ACAATTCAAA
    TGTAGTTTTA TTTCTACTGA AAATTACCAC AGTTGTCTCA GTCTTGTGGC ATCTCAATCT
    GGAACATTTG CAAGTAAAAC TTCTGTGATA ATTGGGGGAA CCGGTAGTTG TGCATTCTCA
    AAATGGGAAC CCGATTCTAC CAAGAAAGAA ATGTCTCTTA AGAACTTTGT GGGCACAGAC
    ATTATTAAAG GTGCAAAGTC ATTTCAGCTG ATCGACAACC TGCTTTTTGT GCTGGATACT
    GATAATGTGC TGAGCTTGTG GGATGCTTAC ACTCTCACTC CTGTGTGGAA CTGGCCCTCT
    CTTCCCGTAG AGCAGTTTGT TCTCACGACA GAAGCGGATT CTCCCTCATC TGTTACCTGG
    CAAGGAATTA CAAATCTCAA ATTAGTCACC CTGACAGCAA CAGCGAAGGA GAAGATGAGA
    AGCCTCATCA TTTACTCTCT GCCTTCGATG GAAACACTGT ACTCTTTGGA AGTATCTAGT
    GTTTCTTCTT TAGTTCAAAC AGGAATCAGC ACGGACACTA TATATCTTCT GGAAGGAATT
    CACAAAAATG ATCCTAACCT ATGTGAAGAC TCTGTGTCTG ACTTAGTACT TAGGTACCTC
    ACAGAGGTGT TACCAGAAAA CAGACTGAGC CGGTTACTTC ACAAGCACAG ATTTGCCGAA
    GCCGAGAGCT TTGCCATTCA GTTCGGACTG GATGTTGAGC TTGTTTATAA GGTCAAATCA
    AATGATATGT TGGAGAAGCT GGCTTTGATT TCCTCGGACA AGAGTGAGCA GTCCAAATGG
    CAGCAACTTG TAGATGAAGC TAAAGAAAAC CTATGCAAAA TCCAGGACGA TGACTTCGTG
    GTGAATTTCT GCCTCAAGGC CCAATGGGTA ACCTATGAAA CCACCCAGGA AATGCTCAGT
    TATGCCAAAA CCAGGCTCAT GAAGAAAGAA GACAGAGCTC TACCTGCCTC TTCTGATGCC
    TTCATGGAGG TGCTAAAAGC TCATGCAAAG TTAACTACAT TTTATGGAGC ATTTGGACCA
    GAAAAATTCA GTGGTAGCTC TTGGATTGAG TTCCTGAATA ACGAAGATGA CCTCAGGGAT
    GTTTTTTTGC AGCTAAGTGA AGGAAACTTC GCTTGTGCTC AGTATCTTTG GCTTAGACAT
    CGGGCTGACT TTGAAAGCAA GTTTGACGTG AAAATGCTGG AGAATTTGCT GAATTCAATA
    TCTACACAAT TCCCCTTGGA AAACCTTTGT TCCTGGTTTA AAAATGAAGT GATTCCGTTT
    GTGAGGAGGA TTGTGCCCGA AGGACAAAAC ATTCTTGCAA AATGGCTAGA ACAAGCATCA
    AGGAACCTAG AGTTAACTGA TAAGGCAAAC TGGCCAGAAA ATGGACTTCA GCTGGCAGAG
    GTGTTCTTCA CTGCAGAAAA AACAGACCGG TTTGGATTCG CATCTTCTTG GCACTGGATT
    TCCTTGGATT ATCAAAACAC AGAGGAAGTC CGCCAGTTAC GGACACTGGT GAGCAAGTTG
    CGGGAGCTGA TCATTCTGCA TAGGAAATAC AACTGCAAGC TGGCCCTCTC GGACTTTGAG
    AAGGAAAATG CCACCACTGT GGTGTTCCGC ATGTTCGACA GGGTCTCGGC CCCAGAGCTG
    ATCCCTTCGG TCTTAGAGAA GTCTGTGAGA GTTTACATTC GCGAACAGAA TCTGCAAGAG
    GAGGAGCTGC TGTTGCTCTA TATAGAGGAT TTACTGAAGA GGTGTAGCTC AAAGTCAATG
    ACTCTCTTCG ACACAGCGTG GGAAGCAAAG GCCATGGCCG TGATAAGATG CTTGTCTGAC
    ACAGATCTCA TCTTTGATGC TGTGCTGAAG ATAATGTACA AGGCGGTGGT TCCCTGGAGT
    GCAGCCGTGG AGCAGCTGGT GAAACAGCAC CTGGAAATGG ACCACCCCAA AGTCAAGTTA
    TTGCAGGAAA GCTACAAACT GATGGAGATG AAGAAACTTT TGCGAGGGTA TGGGATAAGA
    GAGGTGAATC TCCTGAACAA GGAAATAATG AGAGTGATCC GATACATTCT AAAGCAGGAC
    ATCCCGTCTT CTTTAGAAGA TGCTCTGAAG GTGGCCCAGG GGTACAGGCT GTCTGATGAC
    GAAATCTACA GCCTGAGAAT CATCGATCTG ATTGACAGGG AGCAGGGGGG AGATTGCCTC
    CTGCTACTGA AGTCTCTGCC TGCCGCTGAA GCCGAGAAGA CTGCAGAAAG GGTTATCATC
    TGGGCTCGAC TGGCACTGCA GGAGGAGCCT GACGGCTCTG AGGAGGACAA GGCCTGGAGA
    ATATCTGTGG CGAAGACATC GGTGGATATT CTCAAGATCC TGTGTGATAT TCGAAAAGAC
    AATCTGCAGA AGAAGGATGA ATCTGAAGAA TTCCTGAAGC GATTTCAAAT GGTTGCTAGT
    TTACAGGAGA ACTTTGAGGT CTTCCTTCCA TTTGAAGACT ACAGCAACAC CGCCTTGGTA
    GCTGGCCTTC GAGAACAGTA TATTAAGGCA CAGGAAGCTG CACAGGCTGA GCACAAACAC
    AGGGGACCCC CCGGGCCCAC CCCGGCTAGG GGGACACACC TGAGCATCAA GTCAAAGCTG
    CACCGACAGG CACTGGCCCT GCAGGTGTCT GAACAGGAGC TGGAGGCAGA GCTGACCTTG
    AGAGCCTTAA AGGACGGGAA GGTGGTGGCC GCCCTGAGCA AATGCAGAGA TTTACTTAAG
    CATTACTGCA ATGCCGACAC TGGGAGGTTA CTGTTTGTGG TGTGTCAGAA GCTCTGTCAG
    ATGTTGGCTG ACGATGTCCC AATGGTGGCC CCAGGAGGAC TCAGCCTTCC TTCTGAGATC
    CATGACCTCG CATGCCACGC TGTCACCATC TGCAGTCCAG ATTATTTGCT GGATGTTTTA
    GAACTAAGTA AATACACTTT AACCGCTGTG GAGCTTTGCA GACAATGCCA GATGGATGAC
    TGTGGAATGC TGATGAAGGC CGCTCTTGGA ACTCACAAGG ATCCCTATGA AGAGTGGTCC
    TTTAGTGACT TCTTCAGTGA AGATGGAATT GTTCTCGAGT CCCAGGTGGT GCTGCCTGTC
    ATCTATGAAT TGATCTCATC AGTGATGCCC CCTGCAGAAA GCAAAAGACA TCCTTTGGAT
    TCTATAAGTT TGCCATACTG CTCCACTAGT GAAGGAGAAA ACCGTATTCT CCCTCTTGTA
    AGCTCCATCT CTGCTCTGCT CCGGAGCCTC CAGGAATGTA GTCAGTGGGA GCTAGCCCTG
    AGGTTCGTGG TGGGCTCCTT TGGTACCTGC CTGCAGCACT CTATGTCAAA CGTCATGAGT
    ATCAGCTTGA GTAAACAGTT ACTTGGGAAG AACACATTGG CGAACTCCAG GCATATCATT
    ATGGAATTGA AAGAGAAGTC CATCACATTT ATCAGAGAAA ATGCTACGAC ACTCCTGCAT
    AAAGTGTTTA ACTGTCGGGT GGTGGATCTG GACCTCGCCT TGGCTTACTG CACTCTCTTA
    CCTCAAAAGG ATGTATTCGA CAACCTCTGG AAGTTCATAG ATAAAGCGTG GCAGAATTAC
    GACAAAATTC TGGCATTATC TTTGGTGGGC TCTCAGCTGG CAAATCTCTA CCAGGATATT
    GAAACAGGAC TTTGGTTCCA CGAGCTCAGT ATCGATGCCA AGTGGGGTAT TCGTCTTGGT
    AAACTTGGTA TTTCTTTTCA GCCTGCTTTT AGACAAAATT TTCTCACCAA GAAAGACCTT
    ATCAAAGCTC TTGTGAATAA CATAGACATG GACACCAGCC TCATTCTGGA GTATTGCAGC
    ACATTTCAGC TGGACTCTGA CGCTGCCCTT CGGCTGTTCA TTGAAACTCT GCTCCGCAAC
    ACTAGCAGCC AGAGCCAGGG AGATGCTGCC CCGGAGTCCA CAAAGCATCA GCATTCCAAA
    CTCCTGGCGA AAGCCACTGA GCTGGTCCCT TTACTGAAGA ACACAAAGGA TCTGGTGATC
    AGTCTTAGTG AGATCCTCTA TAAGCTGGAC CCATATGATT ATGAAATGAT TGATGTTGTC
    CTGAAAGTTT TAGAACAAGC TAACGAAAAG ATAACCAGTG TGAACATCAA TCAGGCGCTG
    AATCTTCTGA GACACTTGAA GTCATACAGA AGGATCTCCC CGCCAGTGGA CCACGAATAC
    CAGTACGCGC TGGAGCACAT GATCACTCTG CCACCGGCTG CTCACACTAG ACTGCCCTTT
    CACCTAATAC TGTTTGGCAC AGCACAGAAC TTCTGGAAAA TTCTCTCCTC AGAGCTCAGC
    GAAGAGTCGC TGCCAACCCT GCTCTTAATC GCGAAGCTGA TGAAGTTCTC CCTAGATACT
    CTCTATGTGT CTACCGCCAA ACACTTGTTT GAAAAAAACC TGAAGCCAAA GCTCCTGAAG
    TCGGCACAAG CCAGGTCCTC CACCCTGATG AGCAAGGAAG TGGATAAGCT CATGCAGACG
    CTGGAGTCGT ACCTCCTGTC CATCGTCAAC CCCGAGTGGG CTGTCGCCAT TGCCATCAGC
    CTCACCCAGG AAGTCCCAGA AGGCCCCTTC AAGATGTCTA GTTTGAAGTT CTGCCTGTAT
    TTAGCTGAGA GATGGCTACA AAACATCCCA CCTCAGGATG AGACGTGTGA AAAGGCCAAA
    GCCTTGCAGA AGAAGCTCTG CCTCCAGGTC CGGCTCTCAG GAACGGAAGC CGTGCTCATA
    GCCCACAAGC TGAACGATCA GGAATATTTA AGAGTGATAG GGAAGCCAGC GCACCTCATC
    GTGAGCCTCT ACGAACATCC CAGCATAAGC GAAAGGCTTT GTACCACATC TGGCAAAGAT
    TATCCCGATA TCCATACAGC CGCTAAAGAA ATAGCCGAAG TCAATGAAGT TAATTTGGAA
    AAAATCTGGG ACATGTTGTT GGAGAAATGG CTGTGCCCCT CAACGGTGCC CAGTGAGAAA
    GCATCGGAGT TCTTTGAGCT GGAAGAGGAT GAAGTGCTGC ATAGAGTCGT GTATCTCCTC
    CAGGCTCGTC CTGTTGATTA TTGTTCGAGA ATGCTGTTTG TATTTGCCAC TTCAGCTACA
    AGTACACTGG GCATGCGCCA GCTAACGTTT GCCCACAAAG CACGAGCTCT GCAGTGTCTC
    CTCTATTTGG CTGACAAGGA AACAATTGAA TCTCTCTTCA AAAAGCCCAT TAAAGAAATG
    AAATCCTATC TGAAATGTAT AACTTTCCTG GCATCATTTG AGACATTGAA TATTCCCATC
    ACATATGAAT TATTCTGCAA CAGTCCCAAA GAAGGCATGA TTAAGGGCTT GTGGAAGAAC
    CACAGCCACG AACCTATGGC AGTACGATTG GTTGCAGAGC TTTGCTTAGA ATACAAAATC
    TATGACCTGC AGCTTTGGAA TGGGCTCTTG CAGAAGCTTC TGGGCTTCAA CATGATTCCT
    TACCTGAGGA AAGTGTTATC CTGCATTTCT AGCATCCATT CGTTATGGCA GGTGCCCTAC
    TTCAGCAAAG CGTGGCAGCG CGTGATCCAG ATCCCTCTGC TCTCGGCTTC CTGTCCTTTA
    AGACCCAGTC AGTTAGCAGA CTGCTGTGAC AGTCTCGTCG CCATCCTTGA ATGTCCAGTT
    TCAGATGACC TGGACATGAT GGGAGTCGCC AAGCAGTACG TCCAGTTAGA CCTTCCCGCT
    TTTGCTCTGA CCTGCCTGAC GCTCATGCCC CACTCTGAAA AAAGACACCA GCAGATCAAG
    AATTTTCTGA ATTCCTGTGA TGCTCGGATC ATTTTACAGC AGATAGAAGA ACATATGAAC
    ACTGGCCAGC TAGCAGGGTT TTCACATCAG ATTGGAAGTC TGGTTCTGAA TCATGTTGTA
    AATAAGAAGG AATTTGGGAT TTTGGCAAAG ACAAAGTATT TTCAGTTGTT GAAATGCCAC
    GTGATAAACA CCGGCAACGT CACTGAATTG GTAAACTATT TGGCAAATGA CTTCAGTGTA
    GATGAAGCAT CAGCGTTGAT AAATGAGTAT TCAAAGCATT GTGGGAAGCC TGTGCCAGCA
    GATGCAGCTC CCTGTGAGAT CCTGCAGACA TTTCTTGGTG GATCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006530312.1
    CDS297..6464
    Translation

    Target ORF information:

    RefSeq Version XM_006530249.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus kinetochore associated 1 (Kntc1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006530249.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTTGCTTC TAACGAACAA TGGATTTTTT TATATTACAA ACCTCCAGCT TTCACAAATT 
    GAACAGGCCA TTGAGAACAC GGACTTGGAT TCAGCCAAGA AGTTACAAGG ACAATTCAAA
    TGTAGTTTTA TTTCTACTGA AAATTACCAC AGTTGTCTCA GTCTTGTGGC ATCTCAATCT
    GGAACATTTG CAAGTAAAAC TTCTGTGATA ATTGGGGGAA CCGGTAGTTG TGCATTCTCA
    AAATGGGAAC CCGATTCTAC CAAGAAAGAA ATGTCTCTTA AGAACTTTGT GGGCACAGAC
    ATTATTAAAG GTGCAAAGTC ATTTCAGCTG ATCGACAACC TGCTTTTTGT GCTGGATACT
    GATAATGTGC TGAGCTTGTG GGATGCTTAC ACTCTCACTC CTGTGTGGAA CTGGCCCTCT
    CTTCCCGTAG AGCAGTTTGT TCTCACGACA GAAGCGGATT CTCCCTCATC TGTTACCTGG
    CAAGGAATTA CAAATCTCAA ATTAGTCACC CTGACAGCAA CAGCGAAGGA GAAGATGAGA
    AGCCTCATCA TTTACTCTCT GCCTTCGATG GAAACACTGT ACTCTTTGGA AGTATCTAGT
    GTTTCTTCTT TAGTTCAAAC AGGAATCAGC ACGGACACTA TATATCTTCT GGAAGGAATT
    CACAAAAATG ATCCTAACCT ATGTGAAGAC TCTGTGTCTG ACTTAGTACT TAGGTACCTC
    ACAGAGGTGT TACCAGAAAA CAGACTGAGC CGGTTACTTC ACAAGCACAG ATTTGCCGAA
    GCCGAGAGCT TTGCCATTCA GTTCGGACTG GATGTTGAGC TTGTTTATAA GGTCAAATCA
    AATGATATGT TGGAGAAGCT GGCTTTGATT TCCTCGGACA AGAGTGAGCA GTCCAAATGG
    CAGCAACTTG TAGATGAAGC TAAAGAAAAC CTATGCAAAA TCCAGGACGA TGACTTCGTG
    GTGAATTTCT GCCTCAAGGC CCAATGGGTA ACCTATGAAA CCACCCAGGA AATGCTCAGT
    TATGCCAAAA CCAGGCTCAT GAAGAAAGAA GACAGAGCTC TACCTGCCTC TTCTGATGCC
    TTCATGGAGG TGCTAAAAGC TCATGCAAAG TTAACTACAT TTTATGGAGC ATTTGGACCA
    GAAAAATTCA GTGGTAGCTC TTGGATTGAG TTCCTGAATA ACGAAGATGA CCTCAGGGAT
    GTTTTTTTGC AGCTAAGTGA AGGAAACTTC GCTTGTGCTC AGTATCTTTG GCTTAGACAT
    CGGGCTGACT TTGAAAGCAA GTTTGACGTG AAAATGCTGG AGAATTTGCT GAATTCAATA
    TCTACACAAT TCCCCTTGGA AAACCTTTGT TCCTGGTTTA AAAATGAAGT GATTCCGTTT
    GTGAGGAGGA TTGTGCCCGA AGGACAAAAC ATTCTTGCAA AATGGCTAGA ACAAGCATCA
    AGGAACCTAG AGTTAACTGA TAAGGCAAAC TGGCCAGAAA ATGGACTTCA GCTGGCAGAG
    GTGTTCTTCA CTGCAGAAAA AACAGACCGG TTTGGATTCG CATCTTCTTG GCACTGGATT
    TCCTTGGATT ATCAAAACAC AGAGGAAGTC CGCCAGTTAC GGACACTGGT GAGCAAGTTG
    CGGGAGCTGA TCATTCTGCA TAGGAAATAC AACTGCAAGC TGGCCCTCTC GGACTTTGAG
    AAGGAAAATG CCACCACTGT GGTGTTCCGC ATGTTCGACA GGGTCTCGGC CCCAGAGCTG
    ATCCCTTCGG TCTTAGAGAA GTCTGTGAGA GTTTACATTC GCGAACAGAA TCTGCAAGAG
    GAGGAGCTGC TGTTGCTCTA TATAGAGGAT TTACTGAAGA GGTGTAGCTC AAAGTCAATG
    ACTCTCTTCG ACACAGCGTG GGAAGCAAAG GCCATGGCCG TGATAAGATG CTTGTCTGAC
    ACAGATCTCA TCTTTGATGC TGTGCTGAAG ATAATGTACA AGGCGGTGGT TCCCTGGAGT
    GCAGCCGTGG AGCAGCTGGT GAAACAGCAC CTGGAAATGG ACCACCCCAA AGTCAAGTTA
    TTGCAGGAAA GCTACAAACT GATGGAGATG AAGAAACTTT TGCGAGGGTA TGGGATAAGA
    GAGGTGAATC TCCTGAACAA GGAAATAATG AGAGTGATCC GATACATTCT AAAGCAGGAC
    ATCCCGTCTT CTTTAGAAGA TGCTCTGAAG GTGGCCCAGG GGTACAGGCT GTCTGATGAC
    GAAATCTACA GCCTGAGAAT CATCGATCTG ATTGACAGGG AGCAGGGGGG AGATTGCCTC
    CTGCTACTGA AGTCTCTGCC TGCCGCTGAA GCCGAGAAGA CTGCAGAAAG GGTTATCATC
    TGGGCTCGAC TGGCACTGCA GGAGGAGCCT GACGGCTCTG AGGAGGACAA GGCCTGGAGA
    ATATCTGTGG CGAAGACATC GGTGGATATT CTCAAGATCC TGTGTGATAT TCGAAAAGAC
    AATCTGCAGA AGAAGGATGA ATCTGAAGAA TTCCTGAAGC GATTTCAAAT GGTTGCTAGT
    TTACAGGAGA ACTTTGAGGT CTTCCTTCCA TTTGAAGACT ACAGCAACAC CGCCTTGGTA
    GCTGGCCTTC GAGAACAGTA TATTAAGGCA CAGGAAGCTG CACAGGCTGA GCACAAACAC
    AGGGGACCCC CCGGGCCCAC CCCGGCTAGG GGGACACACC TGAGCATCAA GTCAAAGCTG
    CACCGACAGG CACTGGCCCT GCAGGTGTCT GAACAGGAGC TGGAGGCAGA GCTGACCTTG
    AGAGCCTTAA AGGACGGGAA GGTGGTGGCC GCCCTGAGCA AATGCAGAGA TTTACTTAAG
    CATTACTGCA ATGCCGACAC TGGGAGGTTA CTGTTTGTGG TGTGTCAGAA GCTCTGTCAG
    ATGTTGGCTG ACGATGTCCC AATGGTGGCC CCAGGAGGAC TCAGCCTTCC TTCTGAGATC
    CATGACCTCG CATGCCACGC TGTCACCATC TGCAGTCCAG ATTATTTGCT GGATGTTTTA
    GAACTAAGTA AATACACTTT AACCGCTGTG GAGCTTTGCA GACAATGCCA GATGGATGAC
    TGTGGAATGC TGATGAAGGC CGCTCTTGGA ACTCACAAGG ATCCCTATGA AGAGTGGTCC
    TTTAGTGACT TCTTCAGTGA AGATGGAATT GTTCTCGAGT CCCAGGTGGT GCTGCCTGTC
    ATCTATGAAT TGATCTCATC AGTGATGCCC CCTGCAGAAA GCAAAAGACA TCCTTTGGAT
    TCTATAAGTT TGCCATACTG CTCCACTAGT GAAGGAGAAA ACCGTATTCT CCCTCTTGTA
    AGCTCCATCT CTGCTCTGCT CCGGAGCCTC CAGGAATGTA GTCAGTGGGA GCTAGCCCTG
    AGGTTCGTGG TGGGCTCCTT TGGTACCTGC CTGCAGCACT CTATGTCAAA CGTCATGAGT
    ATCAGCTTGA GTAAACAGTT ACTTGGGAAG AACACATTGG CGAACTCCAG GCATATCATT
    ATGGAATTGA AAGAGAAGTC CATCACATTT ATCAGAGAAA ATGCTACGAC ACTCCTGCAT
    AAAGTGTTTA ACTGTCGGGT GGTGGATCTG GACCTCGCCT TGGCTTACTG CACTCTCTTA
    CCTCAAAAGG ATGTATTCGA CAACCTCTGG AAGTTCATAG ATAAAGCGTG GCAGAATTAC
    GACAAAATTC TGGCATTATC TTTGGTGGGC TCTCAGCTGG CAAATCTCTA CCAGGATATT
    GAAACAGGAC TTTGGTTCCA CGAGCTCAGT ATCGATGCCA AGTGGGGTAT TCGTCTTGGT
    AAACTTGGTA TTTCTTTTCA GCCTGCTTTT AGACAAAATT TTCTCACCAA GAAAGACCTT
    ATCAAAGCTC TTGTGAATAA CATAGACATG GACACCAGCC TCATTCTGGA GTATTGCAGC
    ACATTTCAGC TGGACTCTGA CGCTGCCCTT CGGCTGTTCA TTGAAACTCT GCTCCGCAAC
    ACTAGCAGCC AGAGCCAGGG AGATGCTGCC CCGGAGTCCA CAAAGCATCA GCATTCCAAA
    CTCCTGGCGA AAGCCACTGA GCTGGTCCCT TTACTGAAGA ACACAAAGGA TCTGGTGATC
    AGTCTTAGTG AGATCCTCTA TAAGCTGGAC CCATATGATT ATGAAATGAT TGATGTTGTC
    CTGAAAGTTT TAGAACAAGC TAACGAAAAG ATAACCAGTG TGAACATCAA TCAGGCGCTG
    AATCTTCTGA GACACTTGAA GTCATACAGA AGGATCTCCC CGCCAGTGGA CCACGAATAC
    CAGTACGCGC TGGAGCACAT GATCACTCTG CCACCGGCTG CTCACACTAG ACTGCCCTTT
    CACCTAATAC TGTTTGGCAC AGCACAGAAC TTCTGGAAAA TTCTCTCCTC AGAGCTCAGC
    GAAGAGTCGC TGCCAACCCT GCTCTTAATC GCGAAGCTGA TGAAGTTCTC CCTAGATACT
    CTCTATGTGT CTACCGCCAA ACACTTGTTT GAAAAAAACC TGAAGCCAAA GCTCCTGAAG
    TCGGCACAAG CCAGGTCCTC CACCCTGATG AGCAAGGAAG TGGATAAGCT CATGCAGACG
    CTGGAGTCGT ACCTCCTGTC CATCGTCAAC CCCGAGTGGG CTGTCGCCAT TGCCATCAGC
    CTCACCCAGG AAGTCCCAGA AGGCCCCTTC AAGATGTCTA GTTTGAAGTT CTGCCTGTAT
    TTAGCTGAGA GATGGCTACA AAACATCCCA CCTCAGGATG AGACGTGTGA AAAGGCCAAA
    GCCTTGCAGA AGAAGCTCTG CCTCCAGGTC CGGCTCTCAG GAACGGAAGC CGTGCTCATA
    GCCCACAAGC TGAACGATCA GGAATATTTA AGAGTGATAG GGAAGCCAGC GCACCTCATC
    GTGAGCCTCT ACGAACATCC CAGCATAAGC GAAAGGCTTT GTACCACATC TGGCAAAGAT
    TATCCCGATA TCCATACAGC CGCTAAAGAA ATAGCCGAAG TCAATGAAGT TAATTTGGAA
    AAAATCTGGG ACATGTTGTT GGAGAAATGG CTGTGCCCCT CAACGGTGCC CAGTGAGAAA
    GCATCGGAGT TCTTTGAGCT GGAAGAGGAT GAAGTGCTGC ATAGAGTCGT GTATCTCCTC
    CAGGCTCGTC CTGTTGATTA TTGTTCGAGA ATGCTGTTTG TATTTGCCAC TTCAGCTACA
    AGTACACTGG GCATGCGCCA GCTAACGTTT GCCCACAAAG CACGAGCTCT GCAGTGTCTC
    CTCTATTTGG CTGACAAGGA AACAATTGAA TCTCTCTTCA AAAAGCCCAT TAAAGAAATG
    AAATCCTATC TGAAATGTAT AACTTTCCTG GCATCATTTG AGACATTGAA TATTCCCATC
    ACATATGAAT TATTCTGCAA CAGTCCCAAA GAAGGCATGA TTAAGGGCTT GTGGAAGAAC
    CACAGCCACG AACCTATGGC AGTACGATTG GTTGCAGAGC TTTGCTTAGA ATACAAAATC
    TATGACCTGC AGCTTTGGAA TGGGCTCTTG CAGAAGCTTC TGGGCTTCAA CATGATTCCT
    TACCTGAGGA AAGTGTTATC CTGCATTTCT AGCATCCATT CGTTATGGCA GGTGCCCTAC
    TTCAGCAAAG CGTGGCAGCG CGTGATCCAG ATCCCTCTGC TCTCGGCTTC CTGTCCTTTA
    AGACCCAGTC AGTTAGCAGA CTGCTGTGAC AGTCTCGTCG CCATCCTTGA ATGTCCAGTT
    TCAGATGACC TGGACATGAT GGGAGTCGCC AAGCAGTACG TCCAGTTAGA CCTTCCCGCT
    TTTGCTCTGA CCTGCCTGAC GCTCATGCCC CACTCTGAAA AAAGACACCA GCAGATCAAG
    AATTTTCTGA ATTCCTGTGA TGCTCGGATC ATTTTACAGC AGATAGAAGA ACATATGAAC
    ACTGGCCAGC TAGCAGGGTT TTCACATCAG ATTGGAAGTC TGGTTCTGAA TCATGTTGTA
    AATAAGAAGG AATTTGGGAT TTTGGCAAAG ACAAAGTATT TTCAGTTGTT GAAATGCCAC
    GTGATAAACA CCGGCAACGT CACTGAATTG GTAAACTATT TGGCAAATGA CTTCAGTGTA
    GATGAAGCAT CAGCGTTGAT AAATGAGTAT TCAAAGCATT GTGGGAAGCC TGTGCCAGCA
    GATGCAGCTC CCTGTGAGAT CCTGCAGACA TTTCTTGGTG GATCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35845
    Clone ID Related Accession (Same CDS sequence) XM_006530250.3 , XM_006530250.4
    Accession Version XM_006530250.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6099bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product kinetochore-associated protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530250.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTGGAACA ATATTGAGCT GCTAACAAGC GATGACACTG GAAGTGGATG CCTGAGTGTT 
    GGCTCAAGAA AAGAAAACGT GACTGCACTG TACCAAGCCG ACTTGCTTGG GAAGATCTCC
    TCTGAGAAGA CCTCACTGAG TCCGAAGATC CAAGCATTCA GCTTAAGCCA TGGGTTTATA
    ATCGTTGCAG ACCAGTCAGT GATATTGCTT GACAGTATAT GCAGATCACT CCAGTTGTTT
    CTTATCTTCG ATACTGACGT GGATGTGGTC GGCCTGTGTC AAGAAGGAAA GTTTCTCTTT
    GTGGGGGAGA GAAGCGGCAA CTTCCACCTT ATCTACGTCA CATCGAAGCA AACGCTGTTC
    ACCAAGGCTT TTGTGGAGAA AGCTTTGGAC GAGAGTCAGC GAACGTACCG GAATCTCATT
    ATTGAGAAAG ATGGTTCAAA TGAAGGTACC TATTATATGT TGCTTCTAAC GAACAATGGA
    TTTTTTTATA TTACAAACCT CCAGCTTTCA CAAATTGAAC AGGCCATTGA GAACACGGAC
    TTGGATTCAG CCAAGAAGTT ACAAGGACAA TTCAAATGTA GTTTTATTTC TACTGAAAAT
    TACCACAGTT GTCTCAGTCT TGTGGCATCT CAATCTGGAA CATTTGCAAG TAAAACTTCT
    GTGATAATTG GGGGAACCGG TAGTTGTGCA TTCTCAAAAT GGGAACCCGA TTCTACCAAG
    AAAGAAATGT CTCTTAAGAA CTTTGTGGGC ACAGACATTA TTAAAGGTGC AAAGTCATTT
    CAGCTGATCG ACAACCTGCT TTTTGTGCTG GATACTGATA ATGTGCTGAG CTTGTGGGAT
    GCTTACACTC TCACTCCTGT GTGGAACTGG CCCTCTCTTC CCGTAGAGCA GTTTGTTCTC
    ACGACAGAAG CGGATTCTCC CTCATCTGTT ACCTGGCAAG GAATTACAAA TCTCAAATTA
    GTCACCCTGA CAGCAACAGC GAAGGAGAAG ATGAGAAGCC TCATCATTTA CTCTCTGCCT
    TCGATGGAAA CACTGTACTC TTTGGAAGTA TCTAGTGTTT CTTCTTTAGT TCAAACAGGA
    ATCAGCACGG ACACTATATA TCTTCTGGAA GGAATTCACA AAAATGATCC TAACCTATGT
    GAAGACTCTG TGTCTGACTT AGTACTTAGG TACCTCACAG AGGTGTTACC AGAAAACAGA
    CTGAGCCGGT TACTTCACAA GCACAGATTT GCCGAAGCCG AGAGCTTTGC CATTCAGTTC
    GGACTGGATG TTGAGCTTGT TTATAAGGTC AAATCAAATG ATATGTTGGA GAAGCTGGCT
    TTGATTTCCT CGGACAAGAG TGAGCAGTCC AAATGGCAGC AACTTGTAGA TGAAGCTAAA
    GAAAACCTAT GCAAAATCCA GGACGATGAC TTCGTGGTGA ATTTCTGCCT CAAGGCCCAA
    TGGGTAACCT ATGAAACCAC CCAGGAAATG CTCAGTTATG CCAAAACCAG GCTCATGAAG
    AAAGAAGACA GAGCTCTACC TGCCTCTTCT GATGCCTTCA TGGAGGTGCT AAAAGCTCAT
    GCAAAGTTAA CTACATTTTA TGGAGCATTT GGACCAGAAA AATTCAGTGG TAGCTCTTGG
    ATTGAGTTCC TGAATAACGA AGATGACCTC AGGGATGTTT TTTTGCAGCT AAGTGAAGGA
    AACTTCGCTT GTGCTCAGTA TCTTTGGCTT AGACATCGGG CTGACTTTGA AAGCAAGTTT
    GACGTGAAAA TGCTGGAGAA TTTGCTGAAT TCAATATCTA CACAATTCCC CTTGGAAAAC
    CTTTGTTCCT GGTTTAAAAA TGAAGTGATT CCGTTTGTGA GGAGGATTGT GCCCGAAGGA
    CAAAACATTC TTGCAAAATG GCTAGAACAA GCATCAAGGA ACCTAGAGTT AACTGATAAG
    GCAAACTGGC CAGAAAATGG ACTTCAGCTG GCAGAGGTGT TCTTCACTGC AGAAAAAACA
    GACCGGTTTG GATTCGCATC TTCTTGGCAC TGGATTTCCT TGGATTATCA AAACACAGAG
    GAAGTCCGCC AGTTACGGAC ACTGGTGAGC AAGTTGCGGG AGCTGATCAT TCTGCATAGG
    AAATACAACT GCAAGCTGGC CCTCTCGGAC TTTGAGAAGG AAAATGCCAC CACTGTGGTG
    TTCCGCATGT TCGACAGGGT CTCGGCCCCA GAGCTGATCC CTTCGGTCTT AGAGAAGTCT
    GTGAGAGTTT ACATTCGCGA ACAGAATCTG CAAGAGGAGG AGCTGCTGTT GCTCTATATA
    GAGGATTTAC TGAAGAGGTG TAGCTCAAAG TCAATGACTC TCTTCGACAC AGCGTGGGAA
    GCAAAGGCCA TGGCCGTGAT AAGATGCTTG TCTGACACAG ATCTCATCTT TGATGCTGTG
    CTGAAGATAA TGTACAAGGC GGTGGTTCCC TGGAGTGCAG CCGTGGAGCA GCTGGTGAAA
    CAGCACCTGG AAATGGACCA CCCCAAAGTC AAGTTATTGC AGGAAAGCTA CAAACTGATG
    GAGATGAAGA AACTTTTGCG AGGGTATGGG ATAAGAGAGG TGAATCTCCT GAACAAGGAA
    ATAATGAGAG TGATCCGATA CATTCTAAAG CAGGACATCC CGTCTTCTTT AGAAGATGCT
    CTGAAGGTGG CCCAGGGGTA CAGGCTGTCT GATGACGAAA TCTACAGCCT GAGAATCATC
    GATCTGATTG ACAGGGAGCA GGGGGGAGAT TGCCTCCTGC TACTGAAGTC TCTGCCTGCC
    GCTGAAGCCG AGAAGACTGC AGAAAGGGTT ATCATCTGGG CTCGACTGGC ACTGCAGGAG
    GAGCCTGACG GCTCTGAGGA GGACAAGGCC TGGAGAATAT CTGTGGCGAA GACATCGGTG
    GATATTCTCA AGATCCTGTG TGATATTCGA AAAGACAATC TGCAGAAGAA GGATGAATCT
    GAAGAATTCC TGAAGCGATT TCAAATGGTT GCTAGTTTAC AGGAGAACTT TGAGGTCTTC
    CTTCCATTTG AAGACTACAG CAACACCGCC TTGGTAGCTG GCCTTCGAGA ACAGTATATT
    AAGGCACAGG AAGCTGCACA GGCTGAGCAC AAACACAGGG GACCCCCCGG GCCCACCCCG
    GCTAGGGGGA CACACCTGAG CATCAAGTCA AAGCTGCACC GACAGGCACT GGCCCTGCAG
    GTGTCTGAAC AGGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAGGA CGGGAAGGTG
    GTGGCCGCCC TGAGCAAATG CAGAGATTTA CTTAAGCATT ACTGCAATGC CGACACTGGG
    AGGTTACTGT TTGTGGTGTG TCAGAAGCTC TGTCAGATGT TGGCTGACGA TGTCCCAATG
    GTGGCCCCAG GAGGACTCAG CCTTCCTTCT GAGATCCATG ACCTCGCATG CCACGCTGTC
    ACCATCTGCA GTCCAGATTA TTTGCTGGAT GTTTTAGAAC TAAGTAAATA CACTTTAACC
    GCTGTGGAGC TTTGCAGACA ATGCCAGATG GATGACTGTG GAATGCTGAT GAAGGCCGCT
    CTTGGAACTC ACAAGGATCC CTATGAAGAG TGGTCCTTTA GTGACTTCTT CAGTGAAGAT
    GGAATTGTTC TCGAGTCCCA GGTGGTGCTG CCTGTCATCT ATGAATTGAT CTCATCAGTG
    ATGCCCCCTG CAGAAAGCAA AAGACATCCT TTGGATTCTA TAAGTTTGCC ATACTGCTCC
    ACTAGTGAAG GAGAAAACCG TATTCTCCCT CTTGTAAGCT CCATCTCTGC TCTGCTCCGG
    AGCCTCCAGG AATGTAGTCA GTGGGAGCTA GCCCTGAGGT TCGTGGTGGG CTCCTTTGGT
    ACCTGCCTGC AGCACTCTAT GTCAAACGTC ATGAGTATCA GCTTGAGTAA ACAGTTACTT
    GGGAAGAACA CATTGGCGAA CTCCAGGCAT ATCATTATGG AATTGAAAGA GAAGTCCATC
    ACATTTATCA GAGAAAATGC TACGACACTC CTGCATAAAG TGTTTAACTG TCGGGTGGTG
    GATCTGGACC TCGCCTTGGC TTACTGCACT CTCTTACCTC AAAAGGATGT ATTCGACAAC
    CTCTGGAAGT TCATAGATAA AGCGTGGCAG AATTACGACA AAATTCTGGC ATTATCTTTG
    GTGGGCTCTC AGCTGGCAAA TCTCTACCAG GATATTGAAA CAGGACTTTG GTTCCACGAG
    CTCAGTATCG ATGCCAAGTG GGGTATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAGCCT
    GCTTTTAGAC AAAATTTTCT CACCAAGAAA GACCTTATCA AAGCTCTTGT GAATAACATA
    GACATGGACA CCAGCCTCAT TCTGGAGTAT TGCAGCACAT TTCAGCTGGA CTCTGACGCT
    GCCCTTCGGC TGTTCATTGA AACTCTGCTC CGCAACACTA GCAGCCAGAG CCAGGGAGAT
    GCTGCCCCGG AGTCCACAAA GCATCAGCAT TCCAAACTCC TGGCGAAAGC CACTGAGCTG
    GTCCCTTTAC TGAAGAACAC AAAGGATCTG GTGATCAGTC TTAGTGAGAT CCTCTATAAG
    CTGGACCCAT ATGATTATGA AATGATTGAT GTTGTCCTGA AAGTTTTAGA ACAAGCTAAC
    GAAAAGATAA CCAGTGTGAA CATCAATCAG GCGCTGAATC TTCTGAGACA CTTGAAGTCA
    TACAGAAGGA TCTCCCCGCC AGTGGACCAC GAATACCAGT ACGCGCTGGA GCACATGATC
    ACTCTGCCAC CGGCTGCTCA CACTAGACTG CCCTTTCACC TAATACTGTT TGGCACAGCA
    CAGAACTTCT GGAAAATTCT CTCCTCAGAG CTCAGCGAAG AGTCGCTGCC AACCCTGCTC
    TTAATCGCGA AGCTGATGAA GTTCTCCCTA GATACTCTCT ATGTGTCTAC CGCCAAACAC
    TTGTTTGAAA AAAACCTGAA GCCAAAGCTC CTGAAGTCGG CACAAGCCAG GTCCTCCACC
    CTGATGAGCA AGGAAGTGGA TAAGCTCATG CAGACGCTGG AGTCGTACCT CCTGTCCATC
    GTCAACCCCG AGTGGGCTGT CGCCATTGCC ATCAGCCTCA CCCAGGAAGT CCCAGAAGGC
    CCCTTCAAGA TGTCTAGTTT GAAGTTCTGC CTGTATTTAG CTGAGAGATG GCTACAAAAC
    ATCCCACCTC AGGATGAGAC GTGTGAAAAG GCCAAAGCCT TGCAGAAGAA GCTCTGCCTC
    CAGGTCCGGC TCTCAGGAAC GGAAGCCGTG CTCATAGCCC ACAAGCTGAA CGATCAGGAA
    TATTTAAGAG TGATAGGGAA GCCAGCGCAC CTCATCGTGA GCCTCTACGA ACATCCCAGC
    ATAAGCGAAA GGCTTTGTAC CACATCTGGC AAAGATTATC CCGATATCCA TACAGCCGCT
    AAAGAAATAG CCGAAGTCAA TGAAGTTAAT TTGGAAAAAA TCTGGGACAT GTTGTTGGAG
    AAATGGCTGT GCCCCTCAAC GGTGCCCAGT GAGAAAGCAT CGGAGTTCTT TGAGCTGGAA
    GAGGATGAAG TGCTGCATAG AGTCGTGTAT CTCCTCCAGG CTCGTCCTGT TGATTATTGT
    TCGAGAATGC TGTTTGTATT TGCCACTTCA GCTACAAGTA CACTGGGCAT GCGCCAGCTA
    ACGTTTGCCC ACAAAGCACG AGCTCTGCAG TGTCTCCTCT ATTTGGCTGA CAAGGAAACA
    ATTGAATCTC TCTTCAAAAA GCCCATTAAA GAAATGAAAT CCTATCTGAA ATGTATAACT
    TTCCTGGCAT CATTTGAGAC ATTGAATATT CCCATCACAT ATGAATTATT CTGCAACAGT
    CCCAAAGAAG GCATGATTAA GGGCTTGTGG AAGAACCACA GCCACGAACC TATGAGTACA
    CAGGTTAGTC ATGCTTGCGA GCAAGAAGGC ACTGTCGTAC TTCCTTCCAA GGCCCACTCT
    AGAGGGAGAG GTCCCCTTCT CTCGTGCTTA GTTGCCTTTG GGAGACCGGT CACTGATGCC
    GTTGGAGCTG GGACAGGTAT GAGGCCAGCA GGCATCCTGG GTTTTTATTA TTATTATTAT
    TGTTATTGTT GTTATTGTTG TTATTATTGT TATTGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006530313.1
    CDS177..6275
    Translation

    Target ORF information:

    RefSeq Version XM_006530250.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus kinetochore associated 1 (Kntc1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006530250.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTGGAACA ATATTGAGCT GCTAACAAGC GATGACACTG GAAGTGGATG CCTGAGTGTT 
    GGCTCAAGAA AAGAAAACGT GACTGCACTG TACCAAGCCG ACTTGCTTGG GAAGATCTCC
    TCTGAGAAGA CCTCACTGAG TCCGAAGATC CAAGCATTCA GCTTAAGCCA TGGGTTTATA
    ATCGTTGCAG ACCAGTCAGT GATATTGCTT GACAGTATAT GCAGATCACT CCAGTTGTTT
    CTTATCTTCG ATACTGACGT GGATGTGGTC GGCCTGTGTC AAGAAGGAAA GTTTCTCTTT
    GTGGGGGAGA GAAGCGGCAA CTTCCACCTT ATCTACGTCA CATCGAAGCA AACGCTGTTC
    ACCAAGGCTT TTGTGGAGAA AGCTTTGGAC GAGAGTCAGC GAACGTACCG GAATCTCATT
    ATTGAGAAAG ATGGTTCAAA TGAAGGTACC TATTATATGT TGCTTCTAAC GAACAATGGA
    TTTTTTTATA TTACAAACCT CCAGCTTTCA CAAATTGAAC AGGCCATTGA GAACACGGAC
    TTGGATTCAG CCAAGAAGTT ACAAGGACAA TTCAAATGTA GTTTTATTTC TACTGAAAAT
    TACCACAGTT GTCTCAGTCT TGTGGCATCT CAATCTGGAA CATTTGCAAG TAAAACTTCT
    GTGATAATTG GGGGAACCGG TAGTTGTGCA TTCTCAAAAT GGGAACCCGA TTCTACCAAG
    AAAGAAATGT CTCTTAAGAA CTTTGTGGGC ACAGACATTA TTAAAGGTGC AAAGTCATTT
    CAGCTGATCG ACAACCTGCT TTTTGTGCTG GATACTGATA ATGTGCTGAG CTTGTGGGAT
    GCTTACACTC TCACTCCTGT GTGGAACTGG CCCTCTCTTC CCGTAGAGCA GTTTGTTCTC
    ACGACAGAAG CGGATTCTCC CTCATCTGTT ACCTGGCAAG GAATTACAAA TCTCAAATTA
    GTCACCCTGA CAGCAACAGC GAAGGAGAAG ATGAGAAGCC TCATCATTTA CTCTCTGCCT
    TCGATGGAAA CACTGTACTC TTTGGAAGTA TCTAGTGTTT CTTCTTTAGT TCAAACAGGA
    ATCAGCACGG ACACTATATA TCTTCTGGAA GGAATTCACA AAAATGATCC TAACCTATGT
    GAAGACTCTG TGTCTGACTT AGTACTTAGG TACCTCACAG AGGTGTTACC AGAAAACAGA
    CTGAGCCGGT TACTTCACAA GCACAGATTT GCCGAAGCCG AGAGCTTTGC CATTCAGTTC
    GGACTGGATG TTGAGCTTGT TTATAAGGTC AAATCAAATG ATATGTTGGA GAAGCTGGCT
    TTGATTTCCT CGGACAAGAG TGAGCAGTCC AAATGGCAGC AACTTGTAGA TGAAGCTAAA
    GAAAACCTAT GCAAAATCCA GGACGATGAC TTCGTGGTGA ATTTCTGCCT CAAGGCCCAA
    TGGGTAACCT ATGAAACCAC CCAGGAAATG CTCAGTTATG CCAAAACCAG GCTCATGAAG
    AAAGAAGACA GAGCTCTACC TGCCTCTTCT GATGCCTTCA TGGAGGTGCT AAAAGCTCAT
    GCAAAGTTAA CTACATTTTA TGGAGCATTT GGACCAGAAA AATTCAGTGG TAGCTCTTGG
    ATTGAGTTCC TGAATAACGA AGATGACCTC AGGGATGTTT TTTTGCAGCT AAGTGAAGGA
    AACTTCGCTT GTGCTCAGTA TCTTTGGCTT AGACATCGGG CTGACTTTGA AAGCAAGTTT
    GACGTGAAAA TGCTGGAGAA TTTGCTGAAT TCAATATCTA CACAATTCCC CTTGGAAAAC
    CTTTGTTCCT GGTTTAAAAA TGAAGTGATT CCGTTTGTGA GGAGGATTGT GCCCGAAGGA
    CAAAACATTC TTGCAAAATG GCTAGAACAA GCATCAAGGA ACCTAGAGTT AACTGATAAG
    GCAAACTGGC CAGAAAATGG ACTTCAGCTG GCAGAGGTGT TCTTCACTGC AGAAAAAACA
    GACCGGTTTG GATTCGCATC TTCTTGGCAC TGGATTTCCT TGGATTATCA AAACACAGAG
    GAAGTCCGCC AGTTACGGAC ACTGGTGAGC AAGTTGCGGG AGCTGATCAT TCTGCATAGG
    AAATACAACT GCAAGCTGGC CCTCTCGGAC TTTGAGAAGG AAAATGCCAC CACTGTGGTG
    TTCCGCATGT TCGACAGGGT CTCGGCCCCA GAGCTGATCC CTTCGGTCTT AGAGAAGTCT
    GTGAGAGTTT ACATTCGCGA ACAGAATCTG CAAGAGGAGG AGCTGCTGTT GCTCTATATA
    GAGGATTTAC TGAAGAGGTG TAGCTCAAAG TCAATGACTC TCTTCGACAC AGCGTGGGAA
    GCAAAGGCCA TGGCCGTGAT AAGATGCTTG TCTGACACAG ATCTCATCTT TGATGCTGTG
    CTGAAGATAA TGTACAAGGC GGTGGTTCCC TGGAGTGCAG CCGTGGAGCA GCTGGTGAAA
    CAGCACCTGG AAATGGACCA CCCCAAAGTC AAGTTATTGC AGGAAAGCTA CAAACTGATG
    GAGATGAAGA AACTTTTGCG AGGGTATGGG ATAAGAGAGG TGAATCTCCT GAACAAGGAA
    ATAATGAGAG TGATCCGATA CATTCTAAAG CAGGACATCC CGTCTTCTTT AGAAGATGCT
    CTGAAGGTGG CCCAGGGGTA CAGGCTGTCT GATGACGAAA TCTACAGCCT GAGAATCATC
    GATCTGATTG ACAGGGAGCA GGGGGGAGAT TGCCTCCTGC TACTGAAGTC TCTGCCTGCC
    GCTGAAGCCG AGAAGACTGC AGAAAGGGTT ATCATCTGGG CTCGACTGGC ACTGCAGGAG
    GAGCCTGACG GCTCTGAGGA GGACAAGGCC TGGAGAATAT CTGTGGCGAA GACATCGGTG
    GATATTCTCA AGATCCTGTG TGATATTCGA AAAGACAATC TGCAGAAGAA GGATGAATCT
    GAAGAATTCC TGAAGCGATT TCAAATGGTT GCTAGTTTAC AGGAGAACTT TGAGGTCTTC
    CTTCCATTTG AAGACTACAG CAACACCGCC TTGGTAGCTG GCCTTCGAGA ACAGTATATT
    AAGGCACAGG AAGCTGCACA GGCTGAGCAC AAACACAGGG GACCCCCCGG GCCCACCCCG
    GCTAGGGGGA CACACCTGAG CATCAAGTCA AAGCTGCACC GACAGGCACT GGCCCTGCAG
    GTGTCTGAAC AGGAGCTGGA GGCAGAGCTG ACCTTGAGAG CCTTAAAGGA CGGGAAGGTG
    GTGGCCGCCC TGAGCAAATG CAGAGATTTA CTTAAGCATT ACTGCAATGC CGACACTGGG
    AGGTTACTGT TTGTGGTGTG TCAGAAGCTC TGTCAGATGT TGGCTGACGA TGTCCCAATG
    GTGGCCCCAG GAGGACTCAG CCTTCCTTCT GAGATCCATG ACCTCGCATG CCACGCTGTC
    ACCATCTGCA GTCCAGATTA TTTGCTGGAT GTTTTAGAAC TAAGTAAATA CACTTTAACC
    GCTGTGGAGC TTTGCAGACA ATGCCAGATG GATGACTGTG GAATGCTGAT GAAGGCCGCT
    CTTGGAACTC ACAAGGATCC CTATGAAGAG TGGTCCTTTA GTGACTTCTT CAGTGAAGAT
    GGAATTGTTC TCGAGTCCCA GGTGGTGCTG CCTGTCATCT ATGAATTGAT CTCATCAGTG
    ATGCCCCCTG CAGAAAGCAA AAGACATCCT TTGGATTCTA TAAGTTTGCC ATACTGCTCC
    ACTAGTGAAG GAGAAAACCG TATTCTCCCT CTTGTAAGCT CCATCTCTGC TCTGCTCCGG
    AGCCTCCAGG AATGTAGTCA GTGGGAGCTA GCCCTGAGGT TCGTGGTGGG CTCCTTTGGT
    ACCTGCCTGC AGCACTCTAT GTCAAACGTC ATGAGTATCA GCTTGAGTAA ACAGTTACTT
    GGGAAGAACA CATTGGCGAA CTCCAGGCAT ATCATTATGG AATTGAAAGA GAAGTCCATC
    ACATTTATCA GAGAAAATGC TACGACACTC CTGCATAAAG TGTTTAACTG TCGGGTGGTG
    GATCTGGACC TCGCCTTGGC TTACTGCACT CTCTTACCTC AAAAGGATGT ATTCGACAAC
    CTCTGGAAGT TCATAGATAA AGCGTGGCAG AATTACGACA AAATTCTGGC ATTATCTTTG
    GTGGGCTCTC AGCTGGCAAA TCTCTACCAG GATATTGAAA CAGGACTTTG GTTCCACGAG
    CTCAGTATCG ATGCCAAGTG GGGTATTCGT CTTGGTAAAC TTGGTATTTC TTTTCAGCCT
    GCTTTTAGAC AAAATTTTCT CACCAAGAAA GACCTTATCA AAGCTCTTGT GAATAACATA
    GACATGGACA CCAGCCTCAT TCTGGAGTAT TGCAGCACAT TTCAGCTGGA CTCTGACGCT
    GCCCTTCGGC TGTTCATTGA AACTCTGCTC CGCAACACTA GCAGCCAGAG CCAGGGAGAT
    GCTGCCCCGG AGTCCACAAA GCATCAGCAT TCCAAACTCC TGGCGAAAGC CACTGAGCTG
    GTCCCTTTAC TGAAGAACAC AAAGGATCTG GTGATCAGTC TTAGTGAGAT CCTCTATAAG
    CTGGACCCAT ATGATTATGA AATGATTGAT GTTGTCCTGA AAGTTTTAGA ACAAGCTAAC
    GAAAAGATAA CCAGTGTGAA CATCAATCAG GCGCTGAATC TTCTGAGACA CTTGAAGTCA
    TACAGAAGGA TCTCCCCGCC AGTGGACCAC GAATACCAGT ACGCGCTGGA GCACATGATC
    ACTCTGCCAC CGGCTGCTCA CACTAGACTG CCCTTTCACC TAATACTGTT TGGCACAGCA
    CAGAACTTCT GGAAAATTCT CTCCTCAGAG CTCAGCGAAG AGTCGCTGCC AACCCTGCTC
    TTAATCGCGA AGCTGATGAA GTTCTCCCTA GATACTCTCT ATGTGTCTAC CGCCAAACAC
    TTGTTTGAAA AAAACCTGAA GCCAAAGCTC CTGAAGTCGG CACAAGCCAG GTCCTCCACC
    CTGATGAGCA AGGAAGTGGA TAAGCTCATG CAGACGCTGG AGTCGTACCT CCTGTCCATC
    GTCAACCCCG AGTGGGCTGT CGCCATTGCC ATCAGCCTCA CCCAGGAAGT CCCAGAAGGC
    CCCTTCAAGA TGTCTAGTTT GAAGTTCTGC CTGTATTTAG CTGAGAGATG GCTACAAAAC
    ATCCCACCTC AGGATGAGAC GTGTGAAAAG GCCAAAGCCT TGCAGAAGAA GCTCTGCCTC
    CAGGTCCGGC TCTCAGGAAC GGAAGCCGTG CTCATAGCCC ACAAGCTGAA CGATCAGGAA
    TATTTAAGAG TGATAGGGAA GCCAGCGCAC CTCATCGTGA GCCTCTACGA ACATCCCAGC
    ATAAGCGAAA GGCTTTGTAC CACATCTGGC AAAGATTATC CCGATATCCA TACAGCCGCT
    AAAGAAATAG CCGAAGTCAA TGAAGTTAAT TTGGAAAAAA TCTGGGACAT GTTGTTGGAG
    AAATGGCTGT GCCCCTCAAC GGTGCCCAGT GAGAAAGCAT CGGAGTTCTT TGAGCTGGAA
    GAGGATGAAG TGCTGCATAG AGTCGTGTAT CTCCTCCAGG CTCGTCCTGT TGATTATTGT
    TCGAGAATGC TGTTTGTATT TGCCACTTCA GCTACAAGTA CACTGGGCAT GCGCCAGCTA
    ACGTTTGCCC ACAAAGCACG AGCTCTGCAG TGTCTCCTCT ATTTGGCTGA CAAGGAAACA
    ATTGAATCTC TCTTCAAAAA GCCCATTAAA GAAATGAAAT CCTATCTGAA ATGTATAACT
    TTCCTGGCAT CATTTGAGAC ATTGAATATT CCCATCACAT ATGAATTATT CTGCAACAGT
    CCCAAAGAAG GCATGATTAA GGGCTTGTGG AAGAACCACA GCCACGAACC TATGAGTACA
    CAGGTTAGTC ATGCTTGCGA GCAAGAAGGC ACTGTCGTAC TTCCTTCCAA GGCCCACTCT
    AGAGGGAGAG GTCCCCTTCT CTCGTGCTTA GTTGCCTTTG GGAGACCGGT CACTGATGCC
    GTTGGAGCTG GGACAGGTAT GAGGCCAGCA GGCATCCTGG GTTTTTATTA TTATTATTAT
    TGTTATTGTT GTTATTGTTG TTATTATTGT TATTGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.