Spink1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spink1
Entrez Gene ID	20730
Full Name	serine peptidase inhibitor, Kazal type 1
Synonyms	Spink3,p12
General protein information	Preferred Names serine peptidase inhibitor, Kazal type 1 Names serine protease inhibitor Kazal-type 1 prostatic secretory glycoprotein serine peptidase inhibitor, Kazal type 3 serine protease inhibitor Kazal-type 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_009258.5	NP_033284.1	serine protease inhibitor Kazal-type 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPINK1	XP_001160275.1
	SPINK1	XP_001102888.1
Bos taurus (cattle)	SPINK1	NP_001020519.1
Mus musculus (house mouse)	Spink3	NP_033284.1
Homo sapiens (human)	SPINK1	NP_003113.2
Canis lupus familiaris (dog)	SPINK1	XP_850557.1

Gene Ontology
Bibliography

Related articles in PubMed

Forkhead box a2 (FOXA2) is essential for uterine function and fertility.
Kelleher AM, Peng W, Pru JK, Pru CA, DeMayo FJ, Spencer TE
Proceedings of the National Academy of Sciences of the United States of America114(6)E1018-E1026(2017 Feb)

Cathepsin D in pancreatic acinar cells is implicated in cathepsin B and L degradation, but not in autophagic activity.
Mehanna S, Suzuki C, Shibata M, Sunabori T, Imanaka T, Araki K, Yamamura K, Uchiyama Y, Ohmuraya M
Biochemical and biophysical research communications469(3)405-11(2016 Jan)

The nuclear hormone receptor family member NR5A2 controls aspects of multipotent progenitor cell formation and acinar differentiation during pancreatic organogenesis.
Hale MA, Swift GH, Hoang CQ, Deering TG, Masui T, Lee YK, Xue J, MacDonald RJ
Development (Cambridge, England)141(16)3123-33(2014 Aug)

Autophagy regulation in pancreatic acinar cells is independent of epidermal growth factor receptor signaling.
Ozaki N, Fukuchi Y, Tomiyoshi SR, Uehara H, Ida S, Wang J, Araki K, Sibilia M, Baba H, Yamamura K, Ohmuraya M
Biochemical and biophysical research communications446(1)224-30(2014 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

It is a trypsin-specific inhibitor in the pancreas.
Title: Generation and analysis of serine protease inhibitor kazal type 3-cre driver mice.

SPINK3 modulates sperm physiology through a downstream reduction of endogenous NO concentration and independently of SPINK3 trypsin inhibitory activity.
Title: SPINK3 modulates mouse sperm physiology through the reduction of nitric oxide level independently of its trypsin inhibitory activity.

IFN regulatory factor 2 (Irf2) has a regulatory role in trypsinogen5 gene transcription, which is resistant to a major endogenous trypsin inhibitor, Spink3
Title: Characterization of dsRNA-induced pancreatitis model reveals the regulatory role of IFN regulatory factor 2 (Irf2) in trypsinogen5 gene transcription.

uterine-gland-derived SPINK3, as a new paracrine modulator, might play an important role in embryo implantation through its influence on stromal decidualization in mice.
Title: Role of secretory protease inhibitor SPINK3 in mouse uterus during early pregnancy.

Spink3 may act as a growth factor, leading to cell proliferation and regeneration of tissue structure.
Title: Chronic pancreatitis in mice by treatment with choline-deficient ethionine-supplemented diet.

The following Spink1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spink1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13300	NM_009258.5 Latest version!	Mus musculus serine peptidase inhibitor, Kazal type 1 (Spink1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spink1 ( NM_009258.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033284.1 Mus musculus serine peptidase inhibitor, Kazal type 1 (Spink1), mRNA.

Price & Availability↑

CloneID	OMu13300
Clone ID Related Accession (Same CDS sequence)	NM_009258.5
Accession Version	NM_009258.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	serine protease inhibitor Kazal-type 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CA481615.1 and AV048854.1. On Oct 6, 2007 this sequence version replaced NM_009258.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AV048854.1, DV072077.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849378 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGTGG CTGTCATCTT TCTTCTCAGT GCTTTGGCCC TGCTGAGTTT AGCAGGTAAC 
ACTTTTTCAG CTAAGGTGAC TGGAAAAGAG GCTAGTTGCC ATGATGCAGT GGCGGGATGT 
CCCAGAATTT ATGATCCTGT GTGTGGGACT GACGGAATTA CTTATGCCAA TGAATGTGTT 
CTGTGCTTTG AAAACAGGAA ACGCATAGAG CCTGTCCTCA TTCGAAAAGG TGGGCCTTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033284.1
CDS	89..331
Translation	MKVAVIFLLSALALLSLAGNTFSAKVTGKEASCHDAVAGCPRIYDPVCGTDGITYANECVLCFENRKRIEPVLIRKGGPC

Target ORF information:

RefSeq Version	NM_009258.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine peptidase inhibitor, Kazal type 1 (Spink1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009258.5

ORF Insert Sequence:

ATGAAGGTGG CTGTCATCTT TCTTCTCAGT GCTTTGGCCC TGCTGAGTTT AGCAGGTAAC 
ACTTTTTCAG CTAAGGTGAC TGGAAAAGAG GCTAGTTGCC ATGATGCAGT GGCGGGATGT 
CCCAGAATTT ATGATCCTGT GTGTGGGACT GACGGAATTA CTTATGCCAA TGAATGTGTT 
CTGTGCTTTG AAAACAGGAA ACGCATAGAG CCTGTCCTCA TTCGAAAAGG TGGGCCTTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Forkhead box a2 (FOXA2) is essential for uterine function and fertility.
Proceedings of the National Academy of Sciences of the United States of America114(6)E1018-E1026(2017 Feb)
Kelleher AM,Peng W,Pru JK,Pru CA,DeMayo FJ,Spencer TE

Cathepsin D in pancreatic acinar cells is implicated in cathepsin B and L degradation, but not in autophagic activity.
Biochemical and biophysical research communications469(3)405-11(2016 Jan)
Mehanna S,Suzuki C,Shibata M,Sunabori T,Imanaka T,Araki K,Yamamura K,Uchiyama Y,Ohmuraya M

The nuclear hormone receptor family member NR5A2 controls aspects of multipotent progenitor cell formation and acinar differentiation during pancreatic organogenesis.
Development (Cambridge, England)141(16)3123-33(2014 Aug)
Hale MA,Swift GH,Hoang CQ,Deering TG,Masui T,Lee YK,Xue J,MacDonald RJ

Autophagy regulation in pancreatic acinar cells is independent of epidermal growth factor receptor signaling.
Biochemical and biophysical research communications446(1)224-30(2014 Mar)
Ozaki N,Fukuchi Y,Tomiyoshi SR,Uehara H,Ida S,Wang J,Araki K,Sibilia M,Baba H,Yamamura K,Ohmuraya M

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Spink1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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