SPINK1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPINK1
Entrez Gene ID	574092
Full Name	serine peptidase inhibitor, Kazal type 1
General protein information	Preferred Names serine peptidase inhibitor, Kazal type 1 Names serine protease inhibitor Kazal-type 1 pancreatic secretory trypsin inhibitor serine protease inhibitor, Kazal type 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001025348.1	NP_001020519.1	serine protease inhibitor Kazal-type 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPINK1	XP_001160275.1
	SPINK1	XP_001102888.1
Bos taurus (cattle)	SPINK1	NP_001020519.1
Mus musculus (house mouse)	Spink3	NP_033284.1
Homo sapiens (human)	SPINK1	NP_003113.2
Canis lupus familiaris (dog)	SPINK1	XP_850557.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Partially folded bovine pancreatic trypsin inhibitor analogues attain fully native structures when co-crystallized with S195A rat trypsin.
Getun IV, Brown CK, Tulla-Puche J, Ohlendorf D, Woodward C, Barany G
Journal of molecular biology375(3)812-23(2008 Jan)

The structure of the bovine pancreatic secretory trypsin inhibitor--Kazal's inhibitor. 3. Determination of the disulfide bonds and proteolysis by thermolysin.
Guy O, Shapanka R, Greene LJ
The Journal of biological chemistry246(24)7740-7(1971 Dec)

The structure of the bovine pancreatic scretory trypsin inhibitor--Kazal's inhibitor. II. The order of the tryptic peptides.
Greene LJ, Bartelt DC
The Journal of biological chemistry244(10)2646-57(1969 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Partially folded bovine pancreatic trypsin inhibitor analogues attain fully native structures when co-crystallized with S195A rat trypsin.
Title: Partially folded bovine pancreatic trypsin inhibitor analogues attain fully native structures when co-crystallized with S195A rat trypsin.

The following SPINK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPINK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa43638	NM_001025348.1 Latest version!	Bos taurus serine peptidase inhibitor Kazal type 1 (SPINK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPINK1 ( NM_001025348.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001020519.1 Bos taurus serine peptidase inhibitor Kazal type 1 (SPINK1), mRNA.

Price & Availability↑

CloneID	OBa43638
Clone ID Related Accession (Same CDS sequence)	NM_001025348.1
Accession Version	NM_001025348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	serine protease inhibitor Kazal-type 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY911325.1. ##Evidence-Data-START## Transcript exon combination :: AY911325.1, BC102485.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145417, SAMN03145422 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGTGG CAAGCATCTT TCTCCTCACT GCCTTGGTTC TGATGAGTTT ATCTGGTAAC 
AGTGGAGCTA ACATCCTGGG AAGAGAGGCC AAGTGCACAA ATGAAGTGAA CGGATGTCCC 
AGGATCTATA ACCCTGTCTG TGGGACAGAT GGAGTTACTT ATTCTAATGA ATGCTTGTTA 
TGTATGGAAA ATAAGGAACG TCAGACTCCT GTCCTCATTC AAAAATCTGG GCCTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020519.1
CDS	59..298
Translation	MKVASIFLLTALVLMSLSGNSGANILGREAKCTNEVNGCPRIYNPVCGTDGVTYSNECLLCMENKERQTPVLIQKSGPC

Target ORF information:

RefSeq Version	NM_001025348.1
Organism	Bos taurus(cattle)
Definition	Bos taurus serine peptidase inhibitor Kazal type 1 (SPINK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025348.1

ORF Insert Sequence:

ATGAAGGTGG CAAGCATCTT TCTCCTCACT GCCTTGGTTC TGATGAGTTT ATCTGGTAAC 
AGTGGAGCTA ACATCCTGGG AAGAGAGGCC AAGTGCACAA ATGAAGTGAA CGGATGTCCC 
AGGATCTATA ACCCTGTCTG TGGGACAGAT GGAGTTACTT ATTCTAATGA ATGCTTGTTA 
TGTATGGAAA ATAAGGAACG TCAGACTCCT GTCCTCATTC AAAAATCTGG GCCTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Partially folded bovine pancreatic trypsin inhibitor analogues attain fully native structures when co-crystallized with S195A rat trypsin.
Journal of molecular biology375(3)812-23(2008 Jan)
Getun IV,Brown CK,Tulla-Puche J,Ohlendorf D,Woodward C,Barany G

The structure of the bovine pancreatic secretory trypsin inhibitor--Kazal's inhibitor. 3. Determination of the disulfide bonds and proteolysis by thermolysin.
The Journal of biological chemistry246(24)7740-7(1971 Dec)
Guy O,Shapanka R,Greene LJ

The structure of the bovine pancreatic scretory trypsin inhibitor--Kazal's inhibitor. II. The order of the tryptic peptides.
The Journal of biological chemistry244(10)2646-57(1969 May)
Greene LJ,Bartelt DC