Trim27 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trim27
Entrez Gene ID	19720
Full Name	tripartite motif-containing 27
Synonyms	AW538890,Gm19403,Rfp
General protein information	Preferred Names tripartite motif-containing 27 Names zinc finger protein RFP RING-type E3 ubiquitin transferase TRIM27 ret finger protein tripartite motif protein 27 tripartite motif-containing protein 27
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13 A2\|13 7.67 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006516606.2	XP_006516669.1	zinc finger protein RFP isoform X1
XM_006516606.3	XP_006516669.1	zinc finger protein RFP isoform X1
NM_009054.3	NP_033080.2	zinc finger protein RFP

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRIM27	NP_006501.1
Pan troglodytes (chimpanzee)	TRIM27	XP_001140726.2
	TRIM27	XP_001094465.2
Bos taurus (cattle)	TRIM27	NP_001069267.1
Mus musculus (house mouse)	Trim27	NP_033080.2
Canis lupus familiaris (dog)	TRIM27	XP_005642504.1
Gallus gallus (chicken)	TRIM27	NP_001092824.1
Rattus norvegicus (Norway rat)	Trim27	NP_001128446.1

Gene Ontology
Bibliography

Related articles in PubMed

Trim27 interacts with Slx2, is associated with meiotic processes during spermatogenesis.
Zhuang XJ, Tang WH, Feng X, Liu CY, Zhu JL, Yan J, Liu DF, Liu P, Qiao J
Cell cycle (Georgetown, Tex.)15(19)2576-2584(2016 Oct)

Nicotine Induced Murine Spermatozoa Apoptosis via Up-Regulation of Deubiquitinated RIP1 by Trim27 Promoter Hypomethylation.
Nie D, Zhang D, Dai J, Zhang M, Zhao X, Xu W, Chen Z, Wang L, Wang Z, Qiao Z
Biology of reproduction94(2)31(2016 Feb)

Siglec1 suppresses antiviral innate immune response by inducing TBK1 degradation via the ubiquitin ligase TRIM27.
Zheng Q, Hou J, Zhou Y, Yang Y, Xie B, Cao X
Cell research25(10)1121-36(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Trim27 connecting Slx2 may regulate meiotic processes in multiple ways by influencing XY body formation and germ cell proliferation during spermatogenesis.
Title: Trim27 interacts with Slx2, is associated with meiotic processes during spermatogenesis.

our study suggests that nicotine may induce murine spermatozoal apoptosis via the TNF apoptotic pathway through up-regulation of deubiquitinated RIP1 by Trim27 promoter hypomethylation.
Title: Nicotine Induced Murine Spermatozoa Apoptosis via Up-Regulation of Deubiquitinated RIP1 by Trim27 Promoter Hypomethylation.

TRIM27 inhibits VSV infection-induced type I IFN production by promoting TBK1 degradation.
Title: Siglec1 suppresses antiviral innate immune response by inducing TBK1 degradation via the ubiquitin ligase TRIM27.

Data showed that Trim27 deficiency decreases apoptosis and protects dopaminergic neurons in MPTP poisoning, suggesting that Trim27 may be an effective potential target during the treatment of Parkinson's disease
Title: Deficiency of Trim27 protects dopaminergic neurons from apoptosis in the neurotoxin model of Parkinson's disease.

Ret finger protein works as a novel E3 ligase in the Pax7-mediated degradation of MyoD in response to skeletal muscle atrophy.
Title: Ret finger protein mediates Pax7-induced ubiquitination of MyoD in skeletal muscle atrophy.

The following Trim27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trim27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu03423	NM_009054.3 Latest version!	Mus musculus tripartite motif-containing 27 (Trim27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu03423	XM_006516606.3 Latest version!	Mus musculus tripartite motif-containing 27 (Trim27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
View Old Accession Versions >>
OMu03423	XM_006516606.2	Mus musculus tripartite motif-containing 27 (Trim27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trim27 ( NM_009054.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033080.2 Mus musculus tripartite motif-containing 27 (Trim27), mRNA.

Price & Availability↑

CloneID	OMu03423
Clone ID Related Accession (Same CDS sequence)	XM_006516606.2 , XM_006516606.3 , NM_009054.3
Accession Version	NM_009054.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	zinc finger protein RFP
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AI530745.1, BQ573214.1, AK031744.1, CA549428.1 and AC138330.4. On Jul 27, 2011 this sequence version replaced NM_009054.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK031744.1, BC069924.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTCCG GGAGCGTGGC CGAGTGCCTG CAGCAGGAGA CCACCTGCCC CGTGTGCCTT 
CAGTACTTCG TGGAGCCTAT GATGCTCGAC TGCGGCCACA ACATCTGTTG CGCGTGCCTG 
GCCCGCTGCT GGGGTGCCGC GGAGACTAAC GTGTCGTGTC CGCAGTGCCG GGAGACCTTC 
CCGCAGCGCC ACATGCGACC CAACCGGCAC CTGGCCAACG TGACCCAGCT GGTGAAGCAG 
CTGCGCACCG AGCGGCCGTC GGGGCCCGGC GGCGAGATGG GCGTGTGCGA GAAGCACCGG 
GAGCCTCTGA AGCTGTACTG CGAGCAGGAC CAGATGCCCA TCTGCGTGGT GTGCGACCGC 
TCGCGCGAGC ACCGCGGCCA CAGCGTGCTG CCCCTGGAGG AGGCGGTGGA GGGCTTCAAG 
GAGCAAATCC AGAACCGACT GGACCACCTA AGAAGAGTGA AAGACTTGAA GAAGAGACGG 
CGGGCACAGG GGGAGCAGGC CCGAGCTGAG CTTTTGAGCC TGACTCAGAT GGAGAGGGAA 
AAAATTGTCT GGGAGTTTGA GCAGCTCTAT CACTCGTTGA AGGAGCATGA GTACCGCCTG 
TTGGCTCGCC TTGAGGAGCT CGACTTGGCC ATCTATAACA GCATCAACGG GGCCATCACT 
CAGTTCTCCT GCAACATCTC CCACCTCAGT GGCCTGATTG CCCAACTGGA AGAGAAGCAG 
CAGCAGCCCA CGCGGGAGCT CTTACAGGAC ATTGGAGACA CACTGAGCAG GGCTGAAAGA 
ATCAGGATCC CTGAGCCCTG GATCACACCC CCAGATCTAC AAGAGAAAAT CCATATTTTT 
GCCCAAAAAT GTCTGTTTTT GACAGAAAGT CTAAAACAGT TCACAGAAAA GATGCAGTCA 
GATATGGAGA AGATCCAAGA GTTGAGAGAG GCTCAGTTAT ACTCAGTTGA TGTAACCCTG 
GACCCAGACA CAGCCTATCC CAGTCTGATT CTTTCTGATA ATCTTCGGCA AGTACGGTAC 
AGTTACCTCC AGCAGGATCT TCCAGACAAC CCTGAGCGAT TCAATCTGTT TCCCTGTGTC 
TTGGGCTCTC CATGTTTCAT CGCTGGGAGA CATTACTGGG AGGTAGAGGT GGGAGATAAA 
GCCAAGTGGA CCATAGGTGT CTGTGAAGAC TCGGTGTGCA GAAAAGGTGG GGTGACCTCA 
GCCCCCCAGA ATGGATTCTG GGCAGTGTCA TTGTGGTATG GGAAAGAATA TTGGGCTCTT 
ACATCCCCAA TGACTGCACT ACCCCTGCGG ACCCCTCTCC AGAGAGTAGG GATTTTCTTG 
GACTATGATG CTGGTGAGGT TTCCTTCTAC AATGTGACAG AGAGATGCCA TACCTTTACT 
TTCTCTCACG CTACCTTCTG TGGGCCAGTC CGGCCCTACT TCAGCCTAAG CTACTCGGGA 
GGGAAGAGCG CAGCTCCTTT GATCATCTGC CCTATGAGTG GGATTGATGG GTTTTCTGGC 
CATGTTGGGA ATCATGGTCA TTCCATGGAG ACCTCTCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033080.2
CDS	565..2106
Translation	MASGSVAECLQQETTCPVCLQYFVEPMMLDCGHNICCACLARCWGAAETNVSCPQCRETFPQRHMRPNRHLANVTQLVKQLRTERPSGPGGEMGVCEKHREPLKLYCEQDQMPICVVCDRSREHRGHSVLPLEEAVEGFKEQIQNRLDHLRRVKDLKKRRRAQGEQARAELLSLTQMEREKIVWEFEQLYHSLKEHEYRLLARLEELDLAIYNSINGAITQFSCNISHLSGLIAQLEEKQQQPTRELLQDIGDTLSRAERIRIPEPWITPPDLQEKIHIFAQKCLFLTESLKQFTEKMQSDMEKIQELREAQLYSVDVTLDPDTAYPSLILSDNLRQVRYSYLQQDLPDNPERFNLFPCVLGSPCFIAGRHYWEVEVGDKAKWTIGVCEDSVCRKGGVTSAPQNGFWAVSLWYGKEYWALTSPMTALPLRTPLQRVGIFLDYDAGEVSFYNVTERCHTFTFSHATFCGPVRPYFSLSYSGGKSAAPLIICPMSGIDGFSGHVGNHGHSMETSP

Target ORF information:

RefSeq Version	NM_009054.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 27 (Trim27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009054.3

ORF Insert Sequence:

ATGGCCTCCG GGAGCGTGGC CGAGTGCCTG CAGCAGGAGA CCACCTGCCC CGTGTGCCTT 
CAGTACTTCG TGGAGCCTAT GATGCTCGAC TGCGGCCACA ACATCTGTTG CGCGTGCCTG 
GCCCGCTGCT GGGGTGCCGC GGAGACTAAC GTGTCGTGTC CGCAGTGCCG GGAGACCTTC 
CCGCAGCGCC ACATGCGACC CAACCGGCAC CTGGCCAACG TGACCCAGCT GGTGAAGCAG 
CTGCGCACCG AGCGGCCGTC GGGGCCCGGC GGCGAGATGG GCGTGTGCGA GAAGCACCGG 
GAGCCTCTGA AGCTGTACTG CGAGCAGGAC CAGATGCCCA TCTGCGTGGT GTGCGACCGC 
TCGCGCGAGC ACCGCGGCCA CAGCGTGCTG CCCCTGGAGG AGGCGGTGGA GGGCTTCAAG 
GAGCAAATCC AGAACCGACT GGACCACCTA AGAAGAGTGA AAGACTTGAA GAAGAGACGG 
CGGGCACAGG GGGAGCAGGC CCGAGCTGAG CTTTTGAGCC TGACTCAGAT GGAGAGGGAA 
AAAATTGTCT GGGAGTTTGA GCAGCTCTAT CACTCGTTGA AGGAGCATGA GTACCGCCTG 
TTGGCTCGCC TTGAGGAGCT CGACTTGGCC ATCTATAACA GCATCAACGG GGCCATCACT 
CAGTTCTCCT GCAACATCTC CCACCTCAGT GGCCTGATTG CCCAACTGGA AGAGAAGCAG 
CAGCAGCCCA CGCGGGAGCT CTTACAGGAC ATTGGAGACA CACTGAGCAG GGCTGAAAGA 
ATCAGGATCC CTGAGCCCTG GATCACACCC CCAGATCTAC AAGAGAAAAT CCATATTTTT 
GCCCAAAAAT GTCTGTTTTT GACAGAAAGT CTAAAACAGT TCACAGAAAA GATGCAGTCA 
GATATGGAGA AGATCCAAGA GTTGAGAGAG GCTCAGTTAT ACTCAGTTGA TGTAACCCTG 
GACCCAGACA CAGCCTATCC CAGTCTGATT CTTTCTGATA ATCTTCGGCA AGTACGGTAC 
AGTTACCTCC AGCAGGATCT TCCAGACAAC CCTGAGCGAT TCAATCTGTT TCCCTGTGTC 
TTGGGCTCTC CATGTTTCAT CGCTGGGAGA CATTACTGGG AGGTAGAGGT GGGAGATAAA 
GCCAAGTGGA CCATAGGTGT CTGTGAAGAC TCGGTGTGCA GAAAAGGTGG GGTGACCTCA 
GCCCCCCAGA ATGGATTCTG GGCAGTGTCA TTGTGGTATG GGAAAGAATA TTGGGCTCTT 
ACATCCCCAA TGACTGCACT ACCCCTGCGG ACCCCTCTCC AGAGAGTAGG GATTTTCTTG 
GACTATGATG CTGGTGAGGT TTCCTTCTAC AATGTGACAG AGAGATGCCA TACCTTTACT 
TTCTCTCACG CTACCTTCTG TGGGCCAGTC CGGCCCTACT TCAGCCTAAG CTACTCGGGA 
GGGAAGAGCG CAGCTCCTTT GATCATCTGC CCTATGAGTG GGATTGATGG GTTTTCTGGC 
CATGTTGGGA ATCATGGTCA TTCCATGGAG ACCTCTCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim27 ( XM_006516606.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516669.1 Mus musculus tripartite motif-containing 27 (Trim27), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu03423
Clone ID Related Accession (Same CDS sequence)	XM_006516606.2 , XM_006516606.3 , NM_009054.3
Accession Version	XM_006516606.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	zinc finger protein RFP isoform X1
Comment

ATGGCCTCCG GGAGCGTGGC CGAGTGCCTG CAGCAGGAGA CCACCTGCCC CGTGTGCCTT 
CAGTACTTCG TGGAGCCTAT GATGCTCGAC TGCGGCCACA ACATCTGTTG CGCGTGCCTG 
GCCCGCTGCT GGGGTGCCGC GGAGACTAAC GTGTCGTGTC CGCAGTGCCG GGAGACCTTC 
CCGCAGCGCC ACATGCGACC CAACCGGCAC CTGGCCAACG TGACCCAGCT GGTGAAGCAG 
CTGCGCACCG AGCGGCCGTC GGGGCCCGGC GGCGAGATGG GCGTGTGCGA GAAGCACCGG 
GAGCCTCTGA AGCTGTACTG CGAGCAGGAC CAGATGCCCA TCTGCGTGGT GTGCGACCGC 
TCGCGCGAGC ACCGCGGCCA CAGCGTGCTG CCCCTGGAGG AGGCGGTGGA GGGCTTCAAG 
GAGCAAATCC AGAACCGACT GGACCACCTA AGAAGAGTGA AAGACTTGAA GAAGAGACGG 
CGGGCACAGG GGGAGCAGGC CCGAGCTGAG CTTTTGAGCC TGACTCAGAT GGAGAGGGAA 
AAAATTGTCT GGGAGTTTGA GCAGCTCTAT CACTCGTTGA AGGAGCATGA GTACCGCCTG 
TTGGCTCGCC TTGAGGAGCT CGACTTGGCC ATCTATAACA GCATCAACGG GGCCATCACT 
CAGTTCTCCT GCAACATCTC CCACCTCAGT GGCCTGATTG CCCAACTGGA AGAGAAGCAG 
CAGCAGCCCA CGCGGGAGCT CTTACAGGAC ATTGGAGACA CACTGAGCAG GGCTGAAAGA 
ATCAGGATCC CTGAGCCCTG GATCACACCC CCAGATCTAC AAGAGAAAAT CCATATTTTT 
GCCCAAAAAT GTCTGTTTTT GACAGAAAGT CTAAAACAGT TCACAGAAAA GATGCAGTCA 
GATATGGAGA AGATCCAAGA GTTGAGAGAG GCTCAGTTAT ACTCAGTTGA TGTAACCCTG 
GACCCAGACA CAGCCTATCC CAGTCTGATT CTTTCTGATA ATCTTCGGCA AGTACGGTAC 
AGTTACCTCC AGCAGGATCT TCCAGACAAC CCTGAGCGAT TCAATCTGTT TCCCTGTGTC 
TTGGGCTCTC CATGTTTCAT CGCTGGGAGA CATTACTGGG AGGTAGAGGT GGGAGATAAA 
GCCAAGTGGA CCATAGGTGT CTGTGAAGAC TCGGTGTGCA GAAAAGGTGG GGTGACCTCA 
GCCCCCCAGA ATGGATTCTG GGCAGTGTCA TTGTGGTATG GGAAAGAATA TTGGGCTCTT 
ACATCCCCAA TGACTGCACT ACCCCTGCGG ACCCCTCTCC AGAGAGTAGG GATTTTCTTG 
GACTATGATG CTGGTGAGGT TTCCTTCTAC AATGTGACAG AGAGATGCCA TACCTTTACT 
TTCTCTCACG CTACCTTCTG TGGGCCAGTC CGGCCCTACT TCAGCCTAAG CTACTCGGGA 
GGGAAGAGCG CAGCTCCTTT GATCATCTGC CCTATGAGTG GGATTGATGG GTTTTCTGGC 
CATGTTGGGA ATCATGGTCA TTCCATGGAG ACCTCTCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006516669.1

Translation

MASGSVAECLQQETTCPVCLQYFVEPMMLDCGHNICCACLARCWGAAETNVSCPQCRETFPQRHMRPNRHLANVTQLVKQLRTERPSGPGGEMGVCEKHREPLKLYCEQDQMPICVVCDRSREHRGHSVLPLEEAVEGFKEQIQNRLDHLRRVKDLKKRRRAQGEQARAELLSLTQMEREKIVWEFEQLYHSLKEHEYRLLARLEELDLAIYNSINGAITQFSCNISHLSGLIAQLEEKQQQPTRELLQDIGDTLSRAERIRIPEPWITPPDLQEKIHIFAQKCLFLTESLKQFTEKMQSDMEKIQELREAQLYSVDVTLDPDTAYPSLILSDNLRQVRYSYLQQDLPDNPERFNLFPCVLGSPCFIAGRHYWEVEVGDKAKWTIGVCEDSVCRKGGVTSAPQNGFWAVSLWYGKEYWALTSPMTALPLRTPLQRVGIFLDYDAGEVSFYNVTERCHTFTFSHATFCGPVRPYFSLSYSGGKSAAPLIICPMSGIDGFSGHVGNHGHSMETSP

Target ORF information:

RefSeq Version	XM_006516606.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus tripartite motif-containing 27 (Trim27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516606.2

ORF Insert Sequence:

ATGGCCTCCG GGAGCGTGGC CGAGTGCCTG CAGCAGGAGA CCACCTGCCC CGTGTGCCTT 
CAGTACTTCG TGGAGCCTAT GATGCTCGAC TGCGGCCACA ACATCTGTTG CGCGTGCCTG 
GCCCGCTGCT GGGGTGCCGC GGAGACTAAC GTGTCGTGTC CGCAGTGCCG GGAGACCTTC 
CCGCAGCGCC ACATGCGACC CAACCGGCAC CTGGCCAACG TGACCCAGCT GGTGAAGCAG 
CTGCGCACCG AGCGGCCGTC GGGGCCCGGC GGCGAGATGG GCGTGTGCGA GAAGCACCGG 
GAGCCTCTGA AGCTGTACTG CGAGCAGGAC CAGATGCCCA TCTGCGTGGT GTGCGACCGC 
TCGCGCGAGC ACCGCGGCCA CAGCGTGCTG CCCCTGGAGG AGGCGGTGGA GGGCTTCAAG 
GAGCAAATCC AGAACCGACT GGACCACCTA AGAAGAGTGA AAGACTTGAA GAAGAGACGG 
CGGGCACAGG GGGAGCAGGC CCGAGCTGAG CTTTTGAGCC TGACTCAGAT GGAGAGGGAA 
AAAATTGTCT GGGAGTTTGA GCAGCTCTAT CACTCGTTGA AGGAGCATGA GTACCGCCTG 
TTGGCTCGCC TTGAGGAGCT CGACTTGGCC ATCTATAACA GCATCAACGG GGCCATCACT 
CAGTTCTCCT GCAACATCTC CCACCTCAGT GGCCTGATTG CCCAACTGGA AGAGAAGCAG 
CAGCAGCCCA CGCGGGAGCT CTTACAGGAC ATTGGAGACA CACTGAGCAG GGCTGAAAGA 
ATCAGGATCC CTGAGCCCTG GATCACACCC CCAGATCTAC AAGAGAAAAT CCATATTTTT 
GCCCAAAAAT GTCTGTTTTT GACAGAAAGT CTAAAACAGT TCACAGAAAA GATGCAGTCA 
GATATGGAGA AGATCCAAGA GTTGAGAGAG GCTCAGTTAT ACTCAGTTGA TGTAACCCTG 
GACCCAGACA CAGCCTATCC CAGTCTGATT CTTTCTGATA ATCTTCGGCA AGTACGGTAC 
AGTTACCTCC AGCAGGATCT TCCAGACAAC CCTGAGCGAT TCAATCTGTT TCCCTGTGTC 
TTGGGCTCTC CATGTTTCAT CGCTGGGAGA CATTACTGGG AGGTAGAGGT GGGAGATAAA 
GCCAAGTGGA CCATAGGTGT CTGTGAAGAC TCGGTGTGCA GAAAAGGTGG GGTGACCTCA 
GCCCCCCAGA ATGGATTCTG GGCAGTGTCA TTGTGGTATG GGAAAGAATA TTGGGCTCTT 
ACATCCCCAA TGACTGCACT ACCCCTGCGG ACCCCTCTCC AGAGAGTAGG GATTTTCTTG 
GACTATGATG CTGGTGAGGT TTCCTTCTAC AATGTGACAG AGAGATGCCA TACCTTTACT 
TTCTCTCACG CTACCTTCTG TGGGCCAGTC CGGCCCTACT TCAGCCTAAG CTACTCGGGA 
GGGAAGAGCG CAGCTCCTTT GATCATCTGC CCTATGAGTG GGATTGATGG GTTTTCTGGC 
CATGTTGGGA ATCATGGTCA TTCCATGGAG ACCTCTCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim27 ( XM_006516606.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516669.1 Mus musculus tripartite motif-containing 27 (Trim27), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu03423
Clone ID Related Accession (Same CDS sequence)	XM_006516606.2 , XM_006516606.3 , NM_009054.3
Accession Version	XM_006516606.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	zinc finger protein RFP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516606.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCCG GGAGCGTGGC CGAGTGCCTG CAGCAGGAGA CCACCTGCCC CGTGTGCCTT 
CAGTACTTCG TGGAGCCTAT GATGCTCGAC TGCGGCCACA ACATCTGTTG CGCGTGCCTG 
GCCCGCTGCT GGGGTGCCGC GGAGACTAAC GTGTCGTGTC CGCAGTGCCG GGAGACCTTC 
CCGCAGCGCC ACATGCGACC CAACCGGCAC CTGGCCAACG TGACCCAGCT GGTGAAGCAG 
CTGCGCACCG AGCGGCCGTC GGGGCCCGGC GGCGAGATGG GCGTGTGCGA GAAGCACCGG 
GAGCCTCTGA AGCTGTACTG CGAGCAGGAC CAGATGCCCA TCTGCGTGGT GTGCGACCGC 
TCGCGCGAGC ACCGCGGCCA CAGCGTGCTG CCCCTGGAGG AGGCGGTGGA GGGCTTCAAG 
GAGCAAATCC AGAACCGACT GGACCACCTA AGAAGAGTGA AAGACTTGAA GAAGAGACGG 
CGGGCACAGG GGGAGCAGGC CCGAGCTGAG CTTTTGAGCC TGACTCAGAT GGAGAGGGAA 
AAAATTGTCT GGGAGTTTGA GCAGCTCTAT CACTCGTTGA AGGAGCATGA GTACCGCCTG 
TTGGCTCGCC TTGAGGAGCT CGACTTGGCC ATCTATAACA GCATCAACGG GGCCATCACT 
CAGTTCTCCT GCAACATCTC CCACCTCAGT GGCCTGATTG CCCAACTGGA AGAGAAGCAG 
CAGCAGCCCA CGCGGGAGCT CTTACAGGAC ATTGGAGACA CACTGAGCAG GGCTGAAAGA 
ATCAGGATCC CTGAGCCCTG GATCACACCC CCAGATCTAC AAGAGAAAAT CCATATTTTT 
GCCCAAAAAT GTCTGTTTTT GACAGAAAGT CTAAAACAGT TCACAGAAAA GATGCAGTCA 
GATATGGAGA AGATCCAAGA GTTGAGAGAG GCTCAGTTAT ACTCAGTTGA TGTAACCCTG 
GACCCAGACA CAGCCTATCC CAGTCTGATT CTTTCTGATA ATCTTCGGCA AGTACGGTAC 
AGTTACCTCC AGCAGGATCT TCCAGACAAC CCTGAGCGAT TCAATCTGTT TCCCTGTGTC 
TTGGGCTCTC CATGTTTCAT CGCTGGGAGA CATTACTGGG AGGTAGAGGT GGGAGATAAA 
GCCAAGTGGA CCATAGGTGT CTGTGAAGAC TCGGTGTGCA GAAAAGGTGG GGTGACCTCA 
GCCCCCCAGA ATGGATTCTG GGCAGTGTCA TTGTGGTATG GGAAAGAATA TTGGGCTCTT 
ACATCCCCAA TGACTGCACT ACCCCTGCGG ACCCCTCTCC AGAGAGTAGG GATTTTCTTG 
GACTATGATG CTGGTGAGGT TTCCTTCTAC AATGTGACAG AGAGATGCCA TACCTTTACT 
TTCTCTCACG CTACCTTCTG TGGGCCAGTC CGGCCCTACT TCAGCCTAAG CTACTCGGGA 
GGGAAGAGCG CAGCTCCTTT GATCATCTGC CCTATGAGTG GGATTGATGG GTTTTCTGGC 
CATGTTGGGA ATCATGGTCA TTCCATGGAG ACCTCTCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516669.1
CDS	667..2208
Translation	MASGSVAECLQQETTCPVCLQYFVEPMMLDCGHNICCACLARCWGAAETNVSCPQCRETFPQRHMRPNRHLANVTQLVKQLRTERPSGPGGEMGVCEKHREPLKLYCEQDQMPICVVCDRSREHRGHSVLPLEEAVEGFKEQIQNRLDHLRRVKDLKKRRRAQGEQARAELLSLTQMEREKIVWEFEQLYHSLKEHEYRLLARLEELDLAIYNSINGAITQFSCNISHLSGLIAQLEEKQQQPTRELLQDIGDTLSRAERIRIPEPWITPPDLQEKIHIFAQKCLFLTESLKQFTEKMQSDMEKIQELREAQLYSVDVTLDPDTAYPSLILSDNLRQVRYSYLQQDLPDNPERFNLFPCVLGSPCFIAGRHYWEVEVGDKAKWTIGVCEDSVCRKGGVTSAPQNGFWAVSLWYGKEYWALTSPMTALPLRTPLQRVGIFLDYDAGEVSFYNVTERCHTFTFSHATFCGPVRPYFSLSYSGGKSAAPLIICPMSGIDGFSGHVGNHGHSMETSP

Target ORF information:

RefSeq Version	XM_006516606.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 27 (Trim27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516606.3

ORF Insert Sequence:

ATGGCCTCCG GGAGCGTGGC CGAGTGCCTG CAGCAGGAGA CCACCTGCCC CGTGTGCCTT 
CAGTACTTCG TGGAGCCTAT GATGCTCGAC TGCGGCCACA ACATCTGTTG CGCGTGCCTG 
GCCCGCTGCT GGGGTGCCGC GGAGACTAAC GTGTCGTGTC CGCAGTGCCG GGAGACCTTC 
CCGCAGCGCC ACATGCGACC CAACCGGCAC CTGGCCAACG TGACCCAGCT GGTGAAGCAG 
CTGCGCACCG AGCGGCCGTC GGGGCCCGGC GGCGAGATGG GCGTGTGCGA GAAGCACCGG 
GAGCCTCTGA AGCTGTACTG CGAGCAGGAC CAGATGCCCA TCTGCGTGGT GTGCGACCGC 
TCGCGCGAGC ACCGCGGCCA CAGCGTGCTG CCCCTGGAGG AGGCGGTGGA GGGCTTCAAG 
GAGCAAATCC AGAACCGACT GGACCACCTA AGAAGAGTGA AAGACTTGAA GAAGAGACGG 
CGGGCACAGG GGGAGCAGGC CCGAGCTGAG CTTTTGAGCC TGACTCAGAT GGAGAGGGAA 
AAAATTGTCT GGGAGTTTGA GCAGCTCTAT CACTCGTTGA AGGAGCATGA GTACCGCCTG 
TTGGCTCGCC TTGAGGAGCT CGACTTGGCC ATCTATAACA GCATCAACGG GGCCATCACT 
CAGTTCTCCT GCAACATCTC CCACCTCAGT GGCCTGATTG CCCAACTGGA AGAGAAGCAG 
CAGCAGCCCA CGCGGGAGCT CTTACAGGAC ATTGGAGACA CACTGAGCAG GGCTGAAAGA 
ATCAGGATCC CTGAGCCCTG GATCACACCC CCAGATCTAC AAGAGAAAAT CCATATTTTT 
GCCCAAAAAT GTCTGTTTTT GACAGAAAGT CTAAAACAGT TCACAGAAAA GATGCAGTCA 
GATATGGAGA AGATCCAAGA GTTGAGAGAG GCTCAGTTAT ACTCAGTTGA TGTAACCCTG 
GACCCAGACA CAGCCTATCC CAGTCTGATT CTTTCTGATA ATCTTCGGCA AGTACGGTAC 
AGTTACCTCC AGCAGGATCT TCCAGACAAC CCTGAGCGAT TCAATCTGTT TCCCTGTGTC 
TTGGGCTCTC CATGTTTCAT CGCTGGGAGA CATTACTGGG AGGTAGAGGT GGGAGATAAA 
GCCAAGTGGA CCATAGGTGT CTGTGAAGAC TCGGTGTGCA GAAAAGGTGG GGTGACCTCA 
GCCCCCCAGA ATGGATTCTG GGCAGTGTCA TTGTGGTATG GGAAAGAATA TTGGGCTCTT 
ACATCCCCAA TGACTGCACT ACCCCTGCGG ACCCCTCTCC AGAGAGTAGG GATTTTCTTG 
GACTATGATG CTGGTGAGGT TTCCTTCTAC AATGTGACAG AGAGATGCCA TACCTTTACT 
TTCTCTCACG CTACCTTCTG TGGGCCAGTC CGGCCCTACT TCAGCCTAAG CTACTCGGGA 
GGGAAGAGCG CAGCTCCTTT GATCATCTGC CCTATGAGTG GGATTGATGG GTTTTCTGGC 
CATGTTGGGA ATCATGGTCA TTCCATGGAG ACCTCTCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Trim27 interacts with Slx2, is associated with meiotic processes during spermatogenesis.
Cell cycle (Georgetown, Tex.)15(19)2576-2584(2016 Oct)
Zhuang XJ,Tang WH,Feng X,Liu CY,Zhu JL,Yan J,Liu DF,Liu P,Qiao J

Nicotine Induced Murine Spermatozoa Apoptosis via Up-Regulation of Deubiquitinated RIP1 by Trim27 Promoter Hypomethylation.
Biology of reproduction94(2)31(2016 Feb)
Nie D,Zhang D,Dai J,Zhang M,Zhao X,Xu W,Chen Z,Wang L,Wang Z,Qiao Z

Siglec1 suppresses antiviral innate immune response by inducing TBK1 degradation via the ubiquitin ligase TRIM27.
Cell research25(10)1121-36(2015 Oct)
Zheng Q,Hou J,Zhou Y,Yang Y,Xie B,Cao X

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Trim27 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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