Rab12 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rab12
Entrez Gene ID	19328
Full Name	RAB12, member RAS oncogene family
Synonyms	2900054P15Rik,AW555448,C77700
General protein information	Preferred Names RAB12, member RAS oncogene family Names ras-related protein Rab-12 rab-13
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 E1.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_024448.2	NP_077768.2	ras-related protein Rab-12
XM_006523889.1	XP_006523952.1	ras-related protein Rab-12 isoform X1
XM_006523888.3	XP_006523951.1	ras-related protein Rab-12 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rab12	NP_077768.2
Danio rerio (zebrafish)	rab12	NP_001018466.1
Homo sapiens (human)	RAB12	NP_001020471.2
Bos taurus (cattle)	RAB12	NP_001095762.1
	LOC722397	XP_001118552.2
Gallus gallus (chicken)	RAB12	XP_003640848.1
Xenopus tropicalis (tropical clawed frog)	rab12	XP_002937521.2
Pan troglodytes (chimpanzee)	RAB12	XP_001140752.1
Canis lupus familiaris (dog)	RAB12	XP_005623129.1
Rattus norvegicus (Norway rat)	Rab12	NP_037149.1

Gene Ontology
Bibliography

Related articles in PubMed

Rab12 Regulates Retrograde Transport of Mast Cell Secretory Granules by Interacting with the RILP-Dynein Complex.
Efergan A, Azouz NP, Klein O, Noguchi K, Rothenberg ME, Fukuda M, Sagi-Eisenberg R
Journal of immunology (Baltimore, Md. : 1950)196(3)1091-101(2016 Feb)

Dennd3 functions as a guanine nucleotide exchange factor for small GTPase Rab12 in mouse embryonic fibroblasts.
Matsui T, Noguchi K, Fukuda M
The Journal of biological chemistry289(20)13986-95(2014 May)

Rab12 regulates mTORC1 activity and autophagy through controlling the degradation of amino-acid transporter PAT4.
Matsui T, Fukuda M
EMBO reports14(5)450-7(2013 May)

M98K-OPTN induces transferrin receptor degradation and RAB12-mediated autophagic death in retinal ganglion cells.
Sirohi K, Chalasani ML, Sudhakar C, Kumari A, Radha V, Swarup G
Autophagy9(4)510-27(2013 Apr)

Small GTPase Rab12 regulates constitutive degradation of transferrin receptor.
Matsui T, Itoh T, Fukuda M
Traffic (Copenhagen, Denmark)12(10)1432-43(2011 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we identified the small GTPase Rab12 as a novel regulator of mast cell secretory granule transport
Title: Rab12 Regulates Retrograde Transport of Mast Cell Secretory Granules by Interacting with the RILP-Dynein Complex.

Dennd3 not only functions as a Rab12-GEF but also modulates Akt signaling in mouse embryonic fibroblast cells
Title: Dennd3 functions as a guanine nucleotide exchange factor for small GTPase Rab12 in mouse embryonic fibroblasts.

A new mechanism of regulation of mTORC1 signalling and autophagy, that is, quality control of PAT4 by Rab12.
Title: Rab12 regulates mTORC1 activity and autophagy through controlling the degradation of amino-acid transporter PAT4.

Knockdown of Rab12 increased transferrin receptor level and reduced M98K-induced cell death.
Title: M98K-OPTN induces transferrin receptor degradation and RAB12-mediated autophagic death in retinal ganglion cells.

TfR is constitutively degraded by a Rab12-dependent pathway (presumably from recycling endosomes to lysosomes), which is independent of the conventional degradation pathway.
Title: Small GTPase Rab12 regulates constitutive degradation of transferrin receptor.

The following Rab12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rab12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu53321	XM_006523889.1 Latest version!	Mus musculus RAB12, member RAS oncogene family (Rab12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu22079	NM_024448.2 Latest version!	Mus musculus RAB12, member RAS oncogene family (Rab12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OMu53320	XM_006523888.3 Latest version!	Mus musculus RAB12, member RAS oncogene family (Rab12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rab12 ( XM_006523889.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523952.1 Mus musculus RAB12, member RAS oncogene family (Rab12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53321
Clone ID Related Accession (Same CDS sequence)	XM_006523889.1
Accession Version	XM_006523889.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ras-related protein Rab-12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGTGG CATGTCTCCA TCCTTCATCC ATGTCCTTGT CTGGGGCACA GCTGCAGCCA 
AGCCAGCCTT GCTCCACTGG CGTCCTGAGG GACACAGCAG GGCAGGAGCG ATTTAACAGC 
ATTACCTCAG CCTATTACAG AAGTGCCAAG GGGATCATAC TCGTATATGA CATCACCAAG 
AAGGAGACGT TCGATGACTT GCCAAAGTGG ATGAAGATGA TTGACAAGTA TGCTTCAGAA 
GACGCCGAGC TCCTCCTGGT TGGAAATAAG CTGGACTGTG AGACAGACAG AGAAATCTCA 
AGGCAGCAAG GAGAAAAGTT TGCACAGCAG ATAACCGGGA TGCGCTTCTG CGAAGCCAGT 
GCCAAGGACA ATTTCAATGT GGACGAGATC TTTCTGAAAC TTGTTGATGA TATTCTGAAA 
AAGATGCCTC TTGATGTTTT GAGAAACGAG TTATCCAATA GCATCCTTTC TCTACAACCA 
GAGCCTGAGA TCCCACCAGA GCTGCCTCCT CCCAGACCAC ACGTCCGATG CTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523952.1
CDS	9..545
Translation	MLVACLHPSSMSLSGAQLQPSQPCSTGVLRDTAGQERFNSITSAYYRSAKGIILVYDITKKETFDDLPKWMKMIDKYASEDAELLLVGNKLDCETDREISRQQGEKFAQQITGMRFCEASAKDNFNVDEIFLKLVDDILKKMPLDVLRNELSNSILSLQPEPEIPPELPPPRPHVRCC

Target ORF information:

RefSeq Version	XM_006523889.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAB12, member RAS oncogene family (Rab12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523889.1

ORF Insert Sequence:

ATGTTAGTGG CATGTCTCCA TCCTTCATCC ATGTCCTTGT CTGGGGCACA GCTGCAGCCA 
AGCCAGCCTT GCTCCACTGG CGTCCTGAGG GACACAGCAG GGCAGGAGCG ATTTAACAGC 
ATTACCTCAG CCTATTACAG AAGTGCCAAG GGGATCATAC TCGTATATGA CATCACCAAG 
AAGGAGACGT TCGATGACTT GCCAAAGTGG ATGAAGATGA TTGACAAGTA TGCTTCAGAA 
GACGCCGAGC TCCTCCTGGT TGGAAATAAG CTGGACTGTG AGACAGACAG AGAAATCTCA 
AGGCAGCAAG GAGAAAAGTT TGCACAGCAG ATAACCGGGA TGCGCTTCTG CGAAGCCAGT 
GCCAAGGACA ATTTCAATGT GGACGAGATC TTTCTGAAAC TTGTTGATGA TATTCTGAAA 
AAGATGCCTC TTGATGTTTT GAGAAACGAG TTATCCAATA GCATCCTTTC TCTACAACCA 
GAGCCTGAGA TCCCACCAGA GCTGCCTCCT CCCAGACCAC ACGTCCGATG CTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rab12 ( NM_024448.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_077768.2 Mus musculus RAB12, member RAS oncogene family (Rab12), mRNA.

Price & Availability↑

CloneID	OMu22079
Clone ID Related Accession (Same CDS sequence)	NM_024448.2
Accession Version	NM_024448.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	ras-related protein Rab-12
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CK624286.1, AK013690.1, BC027647.1 and BE335991.1. On Jun 2, 2006 this sequence version replaced NM_024448.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7652917.516008.1, SRR7345562.2110877.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849386, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGGATCCCA GCGCCGCGCT GCACCGGCGG CCCGCGGGCG GCAGCCTGGG CGCGGTCTCC 
CCGGCGCTGT CGGGCGGCCA GGCCCGCCGG AGGAAGCAGC CCCCCAGGCC GGCCGACTTC 
AAGCTGCAGG TCATCATCAT CGGCTCCCGC GGCGTGGGCA AGACCAGCCT GATGGAGCGC 
TTCACCGACG ACACGTTCTG CGAGGCCTGC AAGTCCACCG TGGGTGTTGA CTTTAAAATC 
AAGACTGTAG AACTAAGAGG GAAGAAAATT AGATTACAGA TCTGGGACAC AGCAGGGCAG 
GAGCGATTTA ACAGCATTAC CTCAGCCTAT TACAGAAGTG CCAAGGGGAT CATACTCGTA 
TATGACATCA CCAAGAAGGA GACGTTCGAT GACTTGCCAA AGTGGATGAA GATGATTGAC 
AAGTATGCTT CAGAAGACGC CGAGCTCCTC CTGGTTGGAA ATAAGCTGGA CTGTGAGACA 
GACAGAGAAA TCTCAAGGCA GCAAGGAGAA AAGTTTGCAC AGCAGATAAC CGGGATGCGC 
TTCTGCGAAG CCAGTGCCAA GGACAATTTC AATGTGGACG AGATCTTTCT GAAACTTGTT 
GATGATATTC TGAAAAAGAT GCCTCTTGAT GTTTTGAGAA ACGAGTTATC CAATAGCATC 
CTTTCTCTAC AACCAGAGCC TGAGATCCCA CCAGAGCTGC CTCCTCCCAG ACCACACGTC 
CGATGCTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077768.2
CDS	101..832
Translation	MDPSAALHRRPAGGSLGAVSPALSGGQARRRKQPPRPADFKLQVIIIGSRGVGKTSLMERFTDDTFCEACKSTVGVDFKIKTVELRGKKIRLQIWDTAGQERFNSITSAYYRSAKGIILVYDITKKETFDDLPKWMKMIDKYASEDAELLLVGNKLDCETDREISRQQGEKFAQQITGMRFCEASAKDNFNVDEIFLKLVDDILKKMPLDVLRNELSNSILSLQPEPEIPPELPPPRPHVRCC

Target ORF information:

RefSeq Version	NM_024448.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAB12, member RAS oncogene family (Rab12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024448.2

ORF Insert Sequence:

ATGGATCCCA GCGCCGCGCT GCACCGGCGG CCCGCGGGCG GCAGCCTGGG CGCGGTCTCC 
CCGGCGCTGT CGGGCGGCCA GGCCCGCCGG AGGAAGCAGC CCCCCAGGCC GGCCGACTTC 
AAGCTGCAGG TCATCATCAT CGGCTCCCGC GGCGTGGGCA AGACCAGCCT GATGGAGCGC 
TTCACCGACG ACACGTTCTG CGAGGCCTGC AAGTCCACCG TGGGTGTTGA CTTTAAAATC 
AAGACTGTAG AACTAAGAGG GAAGAAAATT AGATTACAGA TCTGGGACAC AGCAGGGCAG 
GAGCGATTTA ACAGCATTAC CTCAGCCTAT TACAGAAGTG CCAAGGGGAT CATACTCGTA 
TATGACATCA CCAAGAAGGA GACGTTCGAT GACTTGCCAA AGTGGATGAA GATGATTGAC 
AAGTATGCTT CAGAAGACGC CGAGCTCCTC CTGGTTGGAA ATAAGCTGGA CTGTGAGACA 
GACAGAGAAA TCTCAAGGCA GCAAGGAGAA AAGTTTGCAC AGCAGATAAC CGGGATGCGC 
TTCTGCGAAG CCAGTGCCAA GGACAATTTC AATGTGGACG AGATCTTTCT GAAACTTGTT 
GATGATATTC TGAAAAAGAT GCCTCTTGAT GTTTTGAGAA ACGAGTTATC CAATAGCATC 
CTTTCTCTAC AACCAGAGCC TGAGATCCCA CCAGAGCTGC CTCCTCCCAG ACCACACGTC 
CGATGCTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rab12 ( XM_006523888.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523951.1 Mus musculus RAB12, member RAS oncogene family (Rab12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53320
Clone ID Related Accession (Same CDS sequence)	XM_006523888.3
Accession Version	XM_006523888.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ras-related protein Rab-12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523888.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTAC CGCTGCTGCG GAGAAGCTGC TTCCCTTCCT CTACTCCCGG CGGCGGCGGC 
GGCGGCGGTA GCGCGGAGCG GGTGGAGGGT GCGCGGGGGC CCGGGGCGCG CGGACGGCGG 
CCCGAGCGCG AGCCGTATGC GTGCATGGAT CCCAGCGCCG CGCTGCACCG GCGGCCCGCG 
GGCGGCAGCC TGGGCGCGGT CTCCCCGGCG CTGTCGGGCG GCCAGGCCCG CCGGAGGAAG 
CAGCCCCCCA GGCCGGCCGA CTTCAAGCTG CAGGTCATCA TCATCGGCTC CCGCGGCGTG 
GGCAAGACCA GCCTGATGGA GCGCTTCACC GACGACACGT TCTGCGAGGC CTGCAAGTCC 
ACCGTGGGGC CTCAACGAAT CATCCTACGG ATGCCCGGGC ATACCCGCCG TGGGCACATC 
TGGCATCCGA GCCGCGCACA GCCCAGGTCT CCGTGGAAGC CCGACCCTCT CTTGTGGCCA 
GACCGAGCTT TTCCGTGGCC TTTCCGCGGG TGTCGGACCC CCGAAGCCCA AACATTCCCT 
CCCGGAGCCT TCACCAGTCA TGAGGTCCGG GTGATGCATC CGCGGAAACT CCCGGTCCGT 
TACTCCTGCT CACCCAGGAA AACGGCTTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523951.1
CDS	46..678
Translation	MLLPLLRRSCFPSSTPGGGGGGGSAERVEGARGPGARGRRPEREPYACMDPSAALHRRPAGGSLGAVSPALSGGQARRRKQPPRPADFKLQVIIIGSRGVGKTSLMERFTDDTFCEACKSTVGPQRIILRMPGHTRRGHIWHPSRAQPRSPWKPDPLLWPDRAFPWPFRGCRTPEAQTFPPGAFTSHEVRVMHPRKLPVRYSCSPRKTAL

Target ORF information:

RefSeq Version	XM_006523888.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAB12, member RAS oncogene family (Rab12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523888.3

ORF Insert Sequence:

ATGCTGCTAC CGCTGCTGCG GAGAAGCTGC TTCCCTTCCT CTACTCCCGG CGGCGGCGGC 
GGCGGCGGTA GCGCGGAGCG GGTGGAGGGT GCGCGGGGGC CCGGGGCGCG CGGACGGCGG 
CCCGAGCGCG AGCCGTATGC GTGCATGGAT CCCAGCGCCG CGCTGCACCG GCGGCCCGCG 
GGCGGCAGCC TGGGCGCGGT CTCCCCGGCG CTGTCGGGCG GCCAGGCCCG CCGGAGGAAG 
CAGCCCCCCA GGCCGGCCGA CTTCAAGCTG CAGGTCATCA TCATCGGCTC CCGCGGCGTG 
GGCAAGACCA GCCTGATGGA GCGCTTCACC GACGACACGT TCTGCGAGGC CTGCAAGTCC 
ACCGTGGGGC CTCAACGAAT CATCCTACGG ATGCCCGGGC ATACCCGCCG TGGGCACATC 
TGGCATCCGA GCCGCGCACA GCCCAGGTCT CCGTGGAAGC CCGACCCTCT CTTGTGGCCA 
GACCGAGCTT TTCCGTGGCC TTTCCGCGGG TGTCGGACCC CCGAAGCCCA AACATTCCCT 
CCCGGAGCCT TCACCAGTCA TGAGGTCCGG GTGATGCATC CGCGGAAACT CCCGGTCCGT 
TACTCCTGCT CACCCAGGAA AACGGCTTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rab12 Regulates Retrograde Transport of Mast Cell Secretory Granules by Interacting with the RILP-Dynein Complex.
Journal of immunology (Baltimore, Md. : 1950)196(3)1091-101(2016 Feb)
Efergan A,Azouz NP,Klein O,Noguchi K,Rothenberg ME,Fukuda M,Sagi-Eisenberg R

Dennd3 functions as a guanine nucleotide exchange factor for small GTPase Rab12 in mouse embryonic fibroblasts.
The Journal of biological chemistry289(20)13986-95(2014 May)
Matsui T,Noguchi K,Fukuda M

Rab12 regulates mTORC1 activity and autophagy through controlling the degradation of amino-acid transporter PAT4.
EMBO reports14(5)450-7(2013 May)
Matsui T,Fukuda M

M98K-OPTN induces transferrin receptor degradation and RAB12-mediated autophagic death in retinal ganglion cells.
Autophagy9(4)510-27(2013 Apr)
Sirohi K,Chalasani ML,Sudhakar C,Kumari A,Radha V,Swarup G

Small GTPase Rab12 regulates constitutive degradation of transferrin receptor.
Traffic (Copenhagen, Denmark)12(10)1432-43(2011 Oct)
Matsui T,Itoh T,Fukuda M