Prps1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prps1
Entrez Gene ID	19139
Full Name	phosphoribosyl pyrophosphate synthetase 1
Synonyms	2310010D17Rik,C76571,C76678,PRS-I,Prps-1
General protein information	Preferred Names phosphoribosyl pyrophosphate synthetase 1 Names ribose-phosphate pyrophosphokinase 1 phosphoribosyl pyrophosphate synthase I phosphoribosyl pyrophosphate synthetase I
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X F1\|X 61.35 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_021463.4	NP_067438.1	ribose-phosphate pyrophosphokinase 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PRPS1	XP_001144504.1
	PRPS1	XP_001095604.1
Gallus gallus (chicken)	PRPS1	XP_004940671.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004890	XP_001688552.1
Oryza sativa (rice)	Os02g0127700	NP_001045763.1
Danio rerio (zebrafish)	prps1b	NP_001070036.1
Arabidopsis thaliana (thale cress)	AT2G35390	NP_850244.1
Canis lupus familiaris (dog)	PRPS1	XP_005641702.1
Bos taurus (cattle)	PRPS1	NP_001039654.1
Rattus norvegicus (Norway rat)	Prps1	NP_058939.1
Drosophila melanogaster (fruit fly)	CG6767	NP_729528.2
Arabidopsis thaliana (thale cress)	PRS2	NP_174516.1
Mus musculus (house mouse)	Prps1	NP_067438.1
Arabidopsis thaliana (thale cress)	AT2G44530	NP_181981.2
Homo sapiens (human)	PRPS1	NP_002755.1

Gene Ontology
Bibliography

Related articles in PubMed

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Bulusu V, Prior N, Snaebjornsson MT, Kuehne A, Sonnen KF, Kress J, Stein F, Schultz C, Sauer U, Aulehla A
Developmental cell40(4)331-341.e4(2017 02)

Phosphoribosylpyrophosphate Synthetase 1 Knockdown Suppresses Tumor Formation of Glioma CD133+ Cells Through Upregulating Cell Apoptosis.
Li C, Yan Z, Cao X, Zhang X, Yang L
Journal of molecular neuroscience : MN60(2)145-56(2016 Oct)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Bowman CE, Zhao L, Hartung T, Wolfgang MJ
Molecular and cellular biology36(15)2089-104(2016 Aug)

High-throughput screening of mouse gene knockouts identifies established and novel skeletal phenotypes.
Brommage R, Liu J, Hansen GM, Kirkpatrick LL, Potter DG, Sands AT, Zambrowicz B, Powell DR, Vogel P
Bone research214034(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PRPS1 knockdown prolonged survival time, reduced tumor volume and upregulated apoptosis in xenograft mouse model of glioma.
Title: Phosphoribosylpyrophosphate Synthetase 1 Knockdown Suppresses Tumor Formation of Glioma CD133+ Cells Through Upregulating Cell Apoptosis.

Only unedited pri-miR-376 RNAs were detected in the cochlea suggesting that the activity of PRPS1 in the inner ear may not be regulated through the editing of miR-376 cluster.
Title: Analysis of miR-376 RNA cluster members in the mouse inner ear.

PRPS1 loss of function mutations cause a type of nonsyndromic X-linked sensorineural deafness, DFN2
Title: Loss-of-function mutations in the PRPS1 gene cause a type of nonsyndromic X-linked sensorineural deafness, DFN2.

The following Prps1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prps1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu12382	NM_021463.4 Latest version!	Mus musculus phosphoribosyl pyrophosphate synthetase 1 (Prps1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prps1 ( NM_021463.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067438.1 Mus musculus phosphoribosyl pyrophosphate synthetase 1 (Prps1), mRNA.

Price & Availability↑

CloneID	OMu12382
Clone ID Related Accession (Same CDS sequence)	NM_021463.4
Accession Version	NM_021463.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	ribose-phosphate pyrophosphokinase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY003422.1, AK166856.1 and BC054772.1. On Oct 21, 2009 this sequence version replaced NM_021463.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC054772.1, AK089009.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCCGAATA TCAAAATCTT CAGCGGGAGC TCCCACCAGG ACTTATCCCA GAAAATCGCT 
GACCGCCTGG GCCTTGAGCT AGGCAAGGTG GTGACTAAGA AATTCAGCAA CCAGGAGACC 
TGTGTGGAAA TTGGCGAAAG CGTTCGTGGA GAAGATGTCT ACATTGTTCA AAGTGGGTGT 
GGTGAGATAA ATGACAATCT AATGGAGCTT TTGATCATGA TTAATGCTTG TAAGATCGCT 
TCAGCCAGCC GGGTTACTGC AGTCATCCCA TGCTTCCCTT ACGCCCGCCA AGATAAGAAG 
GATAAGAGCC GGGCTCCAAT CTCAGCTAAG CTTGTTGCAA ATATGCTATC TGTAGCAGGT 
GCAGATCATA TTATCACCAT GGATCTACAT GCATCTCAAA TTCAGGGTTT CTTTGATATC 
CCGGTGGACA ATTTATATGC AGAGCCAGCT GTCCTAAAGT GGATACGGGA GAATATATCT 
GAGTGGAGAA ACTGTACTAT TGTCTCACCT GATGCTGGTG GAGCCAAAAG AGTGACCTCC 
ATTGCAGACC GGCTGAATGT GGATTTTGCT TTGATTCACA AGGAACGGAA GAAGGCCAAT 
GAAGTGGACC GCATGGTACT TGTGGGAGAT GTGAAGGACC GTGTGGCTAT ACTTGTGGAT 
GACATGGCTG ACACTTGCGG TACAATCTGT CACGCCGCTG ACAAACTTCT CTCAGCTGGC 
GCTACCAGAG TTTATGCTAT CTTGACGCAT GGAATCTTTT CTGGCCCTGC CATTTCTCGA 
ATCAACAATG CATGCTTTGA AGCAGTAGTA GTTACCAATA CCATACCTCA GGAGGACAAG 
ATGAAACACT GCTCCAAGAT ACAGGTGATT GACATCTCTA TGATCCTTGC AGAAGCCATC 
AGGAGAACCC ACAATGGAGA ATCTGTCTCC TACTTGTTCA GCCATGTTCC TTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067438.1
CDS	140..1096
Translation	MPNIKIFSGSSHQDLSQKIADRLGLELGKVVTKKFSNQETCVEIGESVRGEDVYIVQSGCGEINDNLMELLIMINACKIASASRVTAVIPCFPYARQDKKDKSRAPISAKLVANMLSVAGADHIITMDLHASQIQGFFDIPVDNLYAEPAVLKWIRENISEWRNCTIVSPDAGGAKRVTSIADRLNVDFALIHKERKKANEVDRMVLVGDVKDRVAILVDDMADTCGTICHAADKLLSAGATRVYAILTHGIFSGPAISRINNACFEAVVVTNTIPQEDKMKHCSKIQVIDISMILAEAIRRTHNGESVSYLFSHVPL

Target ORF information:

RefSeq Version	NM_021463.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoribosyl pyrophosphate synthetase 1 (Prps1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021463.4

ORF Insert Sequence:

ATGCCGAATA TCAAAATCTT CAGCGGGAGC TCCCACCAGG ACTTATCCCA GAAAATCGCT 
GACCGCCTGG GCCTTGAGCT AGGCAAGGTG GTGACTAAGA AATTCAGCAA CCAGGAGACC 
TGTGTGGAAA TTGGCGAAAG CGTTCGTGGA GAAGATGTCT ACATTGTTCA AAGTGGGTGT 
GGTGAGATAA ATGACAATCT AATGGAGCTT TTGATCATGA TTAATGCTTG TAAGATCGCT 
TCAGCCAGCC GGGTTACTGC AGTCATCCCA TGCTTCCCTT ACGCCCGCCA AGATAAGAAG 
GATAAGAGCC GGGCTCCAAT CTCAGCTAAG CTTGTTGCAA ATATGCTATC TGTAGCAGGT 
GCAGATCATA TTATCACCAT GGATCTACAT GCATCTCAAA TTCAGGGTTT CTTTGATATC 
CCGGTGGACA ATTTATATGC AGAGCCAGCT GTCCTAAAGT GGATACGGGA GAATATATCT 
GAGTGGAGAA ACTGTACTAT TGTCTCACCT GATGCTGGTG GAGCCAAAAG AGTGACCTCC 
ATTGCAGACC GGCTGAATGT GGATTTTGCT TTGATTCACA AGGAACGGAA GAAGGCCAAT 
GAAGTGGACC GCATGGTACT TGTGGGAGAT GTGAAGGACC GTGTGGCTAT ACTTGTGGAT 
GACATGGCTG ACACTTGCGG TACAATCTGT CACGCCGCTG ACAAACTTCT CTCAGCTGGC 
GCTACCAGAG TTTATGCTAT CTTGACGCAT GGAATCTTTT CTGGCCCTGC CATTTCTCGA 
ATCAACAATG CATGCTTTGA AGCAGTAGTA GTTACCAATA CCATACCTCA GGAGGACAAG 
ATGAAACACT GCTCCAAGAT ACAGGTGATT GACATCTCTA TGATCCTTGC AGAAGCCATC 
AGGAGAACCC ACAATGGAGA ATCTGTCTCC TACTTGTTCA GCCATGTTCC TTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Developmental cell40(4)331-341.e4(2017 02)
Bulusu V,Prior N,Snaebjornsson MT,Kuehne A,Sonnen KF,Kress J,Stein F,Schultz C,Sauer U,Aulehla A

Phosphoribosylpyrophosphate Synthetase 1 Knockdown Suppresses Tumor Formation of Glioma CD133+ Cells Through Upregulating Cell Apoptosis.
Journal of molecular neuroscience : MN60(2)145-56(2016 Oct)
Li C,Yan Z,Cao X,Zhang X,Yang L

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Molecular and cellular biology36(15)2089-104(2016 Aug)
Bowman CE,Zhao L,Hartung T,Wolfgang MJ

High-throughput screening of mouse gene knockouts identifies established and novel skeletal phenotypes.
Bone research214034(2014)
Brommage R,Liu J,Hansen GM,Kirkpatrick LL,Potter DG,Sands AT,Zambrowicz B,Powell DR,Vogel P

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Prps1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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