Prm2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prm2
Entrez Gene ID	19119
Full Name	protamine 2
Synonyms	AI528784,Prm-2
General protein information	Preferred Names protamine 2 Names protamine-2 sperm histone P2 sperm protamine P2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16 A1\|16 5.84 cM
Summary	Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis, and are the major DNA-binding proteins in the nucleus of sperm in many vertebrates. They package the sperm DNA into a highly condensed complex in a volume less than 5% of a somatic cell nucleus. Many mammalian species have only one protamine (protamine 1); however, a few species, including human and mouse, have two. This gene encodes protamine 2, which is synthesized as a precursor and then cleaved to give rise to a family of protamine 2 peptides. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_008933.2	NP_032959.1	protamine-2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

IP6K1 is essential for chromatoid body formation and temporal regulation of
Malla AB, Bhandari R
Journal of cell science130(17)2854-2866(2017 Sep)

Purification and characterization of mouse protamines P1 and P2. Amino acid sequence of P2.
Bellv? AR, McKay DJ, Renaux BS, Dixon GH
Biochemistry27(8)2890-7(1988 Apr)

Mouse protamine 2 is synthesized as a precursor whereas mouse protamine 1 is not.
Yelick PC, Balhorn R, Johnson PA, Corzett M, Mazrimas JA, Kleene KC, Hecht NB
Molecular and cellular biology7(6)2173-9(1987 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In CBF-A knockout mice, expression of protamine 2 (PRM2) was reduced and the Prm2 mRNA was prematurely translated in a subset of elongating spermatids.
Title: The transacting factor CBF-A/Hnrnpab binds to the A2RE/RTS element of protamine 2 mRNA and contributes to its translational regulation during mouse spermatogenesis.

Testis-specific miRNA-469 up-regulated in gonadotropin-regulated testicular RNA helicase (GRTH/DDX25)-null mice silences transition protein 2 and protamine 2 messages at sites within coding region: implications of its role in germ cell development.
Title: Testis-specific miRNA-469 up-regulated in gonadotropin-regulated testicular RNA helicase (GRTH/DDX25)-null mice silences transition protein 2 and protamine 2 messages at sites within coding region: implications of its role in germ cell development.

Study shows that protamine 1 and 2 mRNAs, which are specific to the round spermatid stage, are repressed at the two-pronuclei and two-cell stages postfertilization, respectively, in embryos created by round spermatid injection, by distinct mechanisms.
Title: Transcriptional and epigenetic status of protamine 1 and 2 genes following round spermatids injection into mouse oocytes.

Germ cell nuclear factor acts as a repressor of basal transcription on both the Protamine 1 and Protamine 2 promoters.
Title: Reciprocal regulation of the mouse protamine genes by the orphan nuclear receptor germ cell nuclear factor and CREMtau.

direct correlation between the fraction of sperm with haploinsufficiency of PRM2 and the frequency of sperm with damaged DNA. Ultrastructural analysis revealed reduced compaction of the chromatin
Title: Protamine 2 deficiency leads to sperm DNA damage and embryo death in mice.

The following Prm2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prm2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14878	NM_008933.2 Latest version!	Mus musculus protamine 2 (Prm2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prm2 ( NM_008933.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032959.1 Mus musculus protamine 2 (Prm2), mRNA.

Price & Availability↑

CloneID	OMu14878
Clone ID Related Accession (Same CDS sequence)	NM_008933.2
Accession Version	NM_008933.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-14
Organism	Mus musculus(house mouse)
Product	protamine-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK005729.1 and AV207640.1. On Sep 11, 2015 this sequence version replaced NM_008933.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU962013.1, CA464166.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849378 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTTCGCT ACCGAATGAG GAGCCCCAGT GAGGGTCCGC ACCAGGGGCC TGGACAAGAC 
CATGAACGCG AGGAGCAGGG GCAGGGGCAA GGGCTGAGCC CAGAGCGCGT AGAGGACTAT 
GGGAGGACAC ACAGGGGCCA CCACCACCAC AGACACAGGC GCTGCTCTCG TAAGAGGCTA 
CATAGGATCC ACAAGAGGCG TCGGTCATGC AGAAGGCGGA GGAGACACTC CTGCCGCCAC 
AGGAGGCGGC ATCGCAGAGG CTGCAGAAGA TCCCGAAGGA GGAGGAGATG CAGGTGCAGG 
AAATGTAGGA GGCACCATCA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032959.1
CDS	108..431
Translation	MVRYRMRSPSEGPHQGPGQDHEREEQGQGQGLSPERVEDYGRTHRGHHHHRHRRCSRKRLHRIHKRRRSCRRRRRHSCRHRRRHRRGCRRSRRRRRCRCRKCRRHHH

Target ORF information:

RefSeq Version	NM_008933.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protamine 2 (Prm2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008933.2

ORF Insert Sequence:

ATGGTTCGCT ACCGAATGAG GAGCCCCAGT GAGGGTCCGC ACCAGGGGCC TGGACAAGAC 
CATGAACGCG AGGAGCAGGG GCAGGGGCAA GGGCTGAGCC CAGAGCGCGT AGAGGACTAT 
GGGAGGACAC ACAGGGGCCA CCACCACCAC AGACACAGGC GCTGCTCTCG TAAGAGGCTA 
CATAGGATCC ACAAGAGGCG TCGGTCATGC AGAAGGCGGA GGAGACACTC CTGCCGCCAC 
AGGAGGCGGC ATCGCAGAGG CTGCAGAAGA TCCCGAAGGA GGAGGAGATG CAGGTGCAGG 
AAATGTAGGA GGCACCATCA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

IP6K1 is essential for chromatoid body formation and temporal regulation of
Journal of cell science130(17)2854-2866(2017 Sep)
Malla AB,Bhandari R

Purification and characterization of mouse protamines P1 and P2. Amino acid sequence of P2.
Biochemistry27(8)2890-7(1988 Apr)
Bellv? AR,McKay DJ,Renaux BS,Dixon GH

Mouse protamine 2 is synthesized as a precursor whereas mouse protamine 1 is not.
Molecular and cellular biology7(6)2173-9(1987 Jun)
Yelick PC,Balhorn R,Johnson PA,Corzett M,Mazrimas JA,Kleene KC,Hecht NB

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Prm2 cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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