Nedd9 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nedd9
Entrez Gene ID	18003
Full Name	neural precursor cell expressed, developmentally down-regulated gene 9
Synonyms	Cas-L,CasL,HEF1,MEF1,Nedd-9,p105
General protein information	Preferred Names neural precursor cell expressed, developmentally down-regulated gene 9 Names enhancer of filamentation 1 CRK-associated substrate-related protein Crk-associated substrate lymphocyte-specific protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 A3.3-A4

mRNA and Protein(s)

mRNA	Protein	Name
XM_030247179.1	XP_030103039.1	enhancer of filamentation 1 isoform X1
NM_001111324.2	NP_001104794.1	enhancer of filamentation 1 isoform 1
NM_017464.5	NP_059492.3	enhancer of filamentation 1 isoform 2
XM_006516874.3	XP_006516937.1	enhancer of filamentation 1 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	nedd9	NP_001070750.1
Mus musculus (house mouse)	Nedd9	NP_059492.3
Gallus gallus (chicken)	NEDD9	XP_418946.4
Xenopus tropicalis (tropical clawed frog)	nedd9	XP_002932712.1
Canis lupus familiaris (dog)	NEDD9	XP_545342.4
Bos taurus (cattle)	NEDD9	NP_001095317.2
Homo sapiens (human)	NEDD9	NP_006394.1
	NEDD9	XP_001089291.1
Rattus norvegicus (Norway rat)	Nedd9	NP_001011922.1
Danio rerio (zebrafish)	LOC100536178	XP_003200560.1

Gene Ontology
Bibliography

Related articles in PubMed

Actin polymerization-dependent activation of Cas-L promotes immunological synapse stability.
Santos LC, Blair DA, Kumari S, Cammer M, Iskratsch T, Herbin O, Alexandropoulos K, Dustin ML, Sheetz MP
Immunology and cell biology94(10)981-993(2016 11)

Mice null for NEDD9 (HEF1?) display extensive hippocampal dendritic spine loss and cognitive impairment.
Knutson DC, Mitzey AM, Talton LE, Clagett-Dame M
Brain research1632141-55(2016 Feb)

Identification of Nedd9 as a TGF-?-Smad2/3 Target Gene Involved in RANKL-Induced Osteoclastogenesis by Comprehensive Analysis.
Omata Y, Nakamura S, Koyama T, Yasui T, Hirose J, Izawa N, Matsumoto T, Imai Y, Seo S, Kurokawa M, Tsutsumi S, Kadono Y, Morimoto C, Aburatani H, Miyamoto T, Tanaka S
PloS one11(6)e0157992(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a new role for Cas-L in T-cell activation, is reported.
Title: Actin polymerization-dependent activation of Cas-L promotes immunological synapse stability.

NEDD9 is required for the maintenance of dendritic spines in the hippocampus.
Title: Mice null for NEDD9 (HEF1?) display extensive hippocampal dendritic spine loss and cognitive impairment.

These results suggest that Nedd9 is a Smad2/3 target gene implicated in RANKL-induced osteoclastogenesis.
Title: Identification of Nedd9 as a TGF-?-Smad2/3 Target Gene Involved in RANKL-Induced Osteoclastogenesis by Comprehensive Analysis.

NEDD9 has an essential role in development as well as in neoplastic cell proliferation, apoptosis, adhesion, migration and invasion. (Review)
Title: Roles of neural precursor cell expressed, developmentally downregulated 9 in tumor-associated cellular processes (Review).

suggest that Cas-L/NEDD9 plays a pivotal role in the pathophysiology of CIA, and that Cas-L/NEDD9 may be a potential molecular target for the treatment of RA
Title: The role of Cas-L/NEDD9 as a regulator of collagen-induced arthritis in a murine model.

The following Nedd9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nedd9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14178	NM_017464.5 Latest version!	Mus musculus neural precursor cell expressed, developmentally down-regulated gene 9 (Nedd9), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu14247	NM_001111324.2 Latest version!	Mus musculus neural precursor cell expressed, developmentally down-regulated gene 9 (Nedd9), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu48520	XM_006516874.3 Latest version!	Mus musculus neural precursor cell expressed, developmentally down-regulated gene 9 (Nedd9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu48520	XM_030247179.1 Latest version!	Mus musculus neural precursor cell expressed, developmentally down-regulated gene 9 (Nedd9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nedd9 ( NM_017464.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_059492.3 Mus musculus neural precursor cell expressed, developmentally down-regulated gene 9 (Nedd9), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu14178
Clone ID Related Accession (Same CDS sequence)	NM_017464.5
Accession Version	NM_017464.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	enhancer of filamentation 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK033729.1, AK054179.1, AA762918.1, AA198880.1 and AV367231.2. On Dec 14, 2007 this sequence version replaced NM_017464.4. Transcript Variant: This variant (2) contains an alternate 5'-most exon and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is the same length as isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK046357.1, AK054179.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGTACA AGAATCTTAT GGCAAGGGCC TTGTATGACA ACGTCCCTGA GTGTGCTGAG 
GAGCTGGCCT TCCGCAAGGG AGACATCTTA ACTGTCATAG AGCAGAACAC AGGAGGGCTT 
GAGGGATGGT GGCTGTGTTC CCTCCACGGT CGCCAAGGCA TTGTCCCAGG GAACCGGGTG 
AAGCTTCTGA TTGGTCCAGT GCAAGAGACC CCCGGTCATG AGCAGCCTAC TCCTGGACCT 
ATGCATCAGA CCTTTGGCCA ACAGAAACTC TATCAAGTGC CAAATTCCCA GGCAGCATCT 
CGGGATACCA TCTACCAAGT GCCACCCTCC TACCAGAATC AGGGAATTTA CCAAGTACCC 
ACTGGCCATG GCACTCCAGA ACAAGATGTA TATCAAGTAC CACCATCAGT TCAGAGGAAC 
ATTGGCGGCA CTAATGGACC CCTTCTAAGC AAAAAGGTGA TCACCCCAGT GAGGACGGGC 
CATGGCTATG TGTACGAGTA CCCATCCAGA TACCAAAAGG ATGTCTACGA TGTCCCTCCT 
TCCCACAGCA CTCAAGGGGT ATATGACATC CCTCCTTCCT CAGTAAAAGG CCCTGTGTTT 
TCAGTTCCAG TGGGAGAGAT AAAACCTCAA GGGGTATATG ACATTCCCCC CACCCAAGGG 
GTCTATGCCA TTCCACCATC GGCTTGCCGA GATGAGGCAG GGCTCAGGGA AAAGGAATAT 
GATTTCCCTC CTCCAATGAA GCAAGATGGA AAACCAGACA CCAGACCTGA GGGGGTTTAT 
GACATCCCTC CAACCAGCAC CAAGACAGCA GGCAAGGACC TTCACATCAA ATTCCCCTGT 
GATGCTCCAG GAGGTGTCGA ACCAATGGCA CGAAGACACC AGAGCTTTTC CCTGCACCAT 
GCACCCTCTC AGCTGGGACA GTCTGGGGAC ACTCAGAGTG ATGCCTATGA TGTCCCCCGG 
GGAGTTCAGT TTCTGGAGGT ACCAACAGAA ACCAGTGAAA AGGCAAATCC GGAGGAAAGA 
GACGGTGTCT ACGATGTCCC TCTGCACAAC CCAGCAGATG CCAAAGGCTC TCGGGACGTG 
GTAGATGGGA TCAACAGACT GTCTTTCTCC AGCACTGGCA GTACCAGGAG TAACATGTCC 
ACCTCTTCCA CCTCCTCAAA GGAGTCTTCA CTGTCAGCCT CCCCGTCTCA AGACAAAAGG 
CTCCGACTGG ACCCAGACAC AGCCATAGAG AAGCTCTATC GGCTCCAGCA GACCCTGGAG 
ATGGGTGTGT GCAGCCTCAT GTCACTGGTC ACCACAGACT GGAGGTGCTA CGGATACATG 
GAAAGGCACA TCAATGAGAT CCGCACCGCG GTGGACAAAG TAGAGCTGTT CTTACGAGAA 
TACCTCCATT TTGCCAAGGG AGCTTTAGCC AATGCCTCCT GCCTCCCAGA ACTGGTCCTC 
CACAACAAAA TGAAGCGGGA ACTCCAAAGA GTAGAAGATT CCCACCAGAT TCTAAGCCAA 
ACCAGCCATG ACTTGAATGA ATGCAGCTGG TCCCTGAATA TTTTAGCTAT CAATAAGCCC 
CAAAATAAGT GTGATGACCT AGACCGGTTT GTGATGGTCG CCAAGACAGT GCCAGACGAC 
GCCAAACAAC TGACCACCAC CATCAGCACC TACGCGGAGA CCCTCTTTAG AGCAGATCCT 
GCCAATTCCC ATCTGAAGAA TGGGCCCAAC AGCATCATGA ACTCAAGCGA GTACACACAT 
CCGGGCTCCC AGATGCAGCC ACTGCATCCT GGTGACTACA AAGCCCAGGT CCACAGTAAG 
CCGTTGCCTC CTAGTCTAAG CAAGGACCAG CCACCAGACT GCGGTAGCAG TGACGGTTCT 
GAGCGGAGTT GGATGGATGA TTATGATTAT GTTCACCTAC AGGGCAAGGA GGAGTTTGAG 
CGACAGCAGA AGGAGCTCTT GGAAAAGGAG AACATCATGA AGCAGAGTAA GGCGCAGCTG 
GAGCATCACC AGCTGAGTCA GTTCCAGCTG TTGGAACAAG AGATCACCAA GCCTGTGGAG 
AATGACATCT CTAAATGGAA GCCCTCTCAG AGCCTCCCAA CCACCAACAA CAGTGTGGGT 
GCTCAGGATA GGCAGTTGCT TTGCTTCTAC TATGACCAGT GCGAGACCCA TTTCATTTCC 
CTACTCAACG CCATCGACGC CCTCTTCAGC TGCGTCAGCT CAGCCCAACC CCCACGGATC 
TTTGTGGCGC ACAGCAAGTT TGTCATTCTT AGTGCGCACA AACTGGTGTT CATTGGAGAC 
ACTCTGACAA GGCAGGTGGC TGCCCAGGAC ATTCGCAACA AAGTCAGGAA CTCCAGCAAC 
CAGCTCTGCG AACAGCTCAA GACGATAGTG ATGGCGACCA AAATGGCCGC CCTCCACTAC 
CCCAGTACCA CCGCCTTGCA GGAAATGGTG CACCAGGTGA CAGACCTGTC CAGAAATGCT 
CAGCTGTTTA AGCGTTCCTT GCTGGAGATG GCCACCTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059492.3
CDS	138..2639
Translation	MKYKNLMARALYDNVPECAEELAFRKGDILTVIEQNTGGLEGWWLCSLHGRQGIVPGNRVKLLIGPVQETPGHEQPTPGPMHQTFGQQKLYQVPNSQAASRDTIYQVPPSYQNQGIYQVPTGHGTPEQDVYQVPPSVQRNIGGTNGPLLSKKVITPVRTGHGYVYEYPSRYQKDVYDVPPSHSTQGVYDIPPSSVKGPVFSVPVGEIKPQGVYDIPPTQGVYAIPPSACRDEAGLREKEYDFPPPMKQDGKPDTRPEGVYDIPPTSTKTAGKDLHIKFPCDAPGGVEPMARRHQSFSLHHAPSQLGQSGDTQSDAYDVPRGVQFLEVPTETSEKANPEERDGVYDVPLHNPADAKGSRDVVDGINRLSFSSTGSTRSNMSTSSTSSKESSLSASPSQDKRLRLDPDTAIEKLYRLQQTLEMGVCSLMSLVTTDWRCYGYMERHINEIRTAVDKVELFLREYLHFAKGALANASCLPELVLHNKMKRELQRVEDSHQILSQTSHDLNECSWSLNILAINKPQNKCDDLDRFVMVAKTVPDDAKQLTTTISTYAETLFRADPANSHLKNGPNSIMNSSEYTHPGSQMQPLHPGDYKAQVHSKPLPPSLSKDQPPDCGSSDGSERSWMDDYDYVHLQGKEEFERQQKELLEKENIMKQSKAQLEHHQLSQFQLLEQEITKPVENDISKWKPSQSLPTTNNSVGAQDRQLLCFYYDQCETHFISLLNAIDALFSCVSSAQPPRIFVAHSKFVILSAHKLVFIGDTLTRQVAAQDIRNKVRNSSNQLCEQLKTIVMATKMAALHYPSTTALQEMVHQVTDLSRNAQLFKRSLLEMATF

Target ORF information:

RefSeq Version	NM_017464.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus neural precursor cell expressed, developmentally down-regulated gene 9 (Nedd9), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017464.5

ORF Insert Sequence:

ATGAAGTACA AGAATCTTAT GGCAAGGGCC TTGTATGACA ACGTCCCTGA GTGTGCTGAG 
GAGCTGGCCT TCCGCAAGGG AGACATCTTA ACTGTCATAG AGCAGAACAC AGGAGGGCTT 
GAGGGATGGT GGCTGTGTTC CCTCCACGGT CGCCAAGGCA TTGTCCCAGG GAACCGGGTG 
AAGCTTCTGA TTGGTCCAGT GCAAGAGACC CCCGGTCATG AGCAGCCTAC TCCTGGACCT 
ATGCATCAGA CCTTTGGCCA ACAGAAACTC TATCAAGTGC CAAATTCCCA GGCAGCATCT 
CGGGATACCA TCTACCAAGT GCCACCCTCC TACCAGAATC AGGGAATTTA CCAAGTACCC 
ACTGGCCATG GCACTCCAGA ACAAGATGTA TATCAAGTAC CACCATCAGT TCAGAGGAAC 
ATTGGCGGCA CTAATGGACC CCTTCTAAGC AAAAAGGTGA TCACCCCAGT GAGGACGGGC 
CATGGCTATG TGTACGAGTA CCCATCCAGA TACCAAAAGG ATGTCTACGA TGTCCCTCCT 
TCCCACAGCA CTCAAGGGGT ATATGACATC CCTCCTTCCT CAGTAAAAGG CCCTGTGTTT 
TCAGTTCCAG TGGGAGAGAT AAAACCTCAA GGGGTATATG ACATTCCCCC CACCCAAGGG 
GTCTATGCCA TTCCACCATC GGCTTGCCGA GATGAGGCAG GGCTCAGGGA AAAGGAATAT 
GATTTCCCTC CTCCAATGAA GCAAGATGGA AAACCAGACA CCAGACCTGA GGGGGTTTAT 
GACATCCCTC CAACCAGCAC CAAGACAGCA GGCAAGGACC TTCACATCAA ATTCCCCTGT 
GATGCTCCAG GAGGTGTCGA ACCAATGGCA CGAAGACACC AGAGCTTTTC CCTGCACCAT 
GCACCCTCTC AGCTGGGACA GTCTGGGGAC ACTCAGAGTG ATGCCTATGA TGTCCCCCGG 
GGAGTTCAGT TTCTGGAGGT ACCAACAGAA ACCAGTGAAA AGGCAAATCC GGAGGAAAGA 
GACGGTGTCT ACGATGTCCC TCTGCACAAC CCAGCAGATG CCAAAGGCTC TCGGGACGTG 
GTAGATGGGA TCAACAGACT GTCTTTCTCC AGCACTGGCA GTACCAGGAG TAACATGTCC 
ACCTCTTCCA CCTCCTCAAA GGAGTCTTCA CTGTCAGCCT CCCCGTCTCA AGACAAAAGG 
CTCCGACTGG ACCCAGACAC AGCCATAGAG AAGCTCTATC GGCTCCAGCA GACCCTGGAG 
ATGGGTGTGT GCAGCCTCAT GTCACTGGTC ACCACAGACT GGAGGTGCTA CGGATACATG 
GAAAGGCACA TCAATGAGAT CCGCACCGCG GTGGACAAAG TAGAGCTGTT CTTACGAGAA 
TACCTCCATT TTGCCAAGGG AGCTTTAGCC AATGCCTCCT GCCTCCCAGA ACTGGTCCTC 
CACAACAAAA TGAAGCGGGA ACTCCAAAGA GTAGAAGATT CCCACCAGAT TCTAAGCCAA 
ACCAGCCATG ACTTGAATGA ATGCAGCTGG TCCCTGAATA TTTTAGCTAT CAATAAGCCC 
CAAAATAAGT GTGATGACCT AGACCGGTTT GTGATGGTCG CCAAGACAGT GCCAGACGAC 
GCCAAACAAC TGACCACCAC CATCAGCACC TACGCGGAGA CCCTCTTTAG AGCAGATCCT 
GCCAATTCCC ATCTGAAGAA TGGGCCCAAC AGCATCATGA ACTCAAGCGA GTACACACAT 
CCGGGCTCCC AGATGCAGCC ACTGCATCCT GGTGACTACA AAGCCCAGGT CCACAGTAAG 
CCGTTGCCTC CTAGTCTAAG CAAGGACCAG CCACCAGACT GCGGTAGCAG TGACGGTTCT 
GAGCGGAGTT GGATGGATGA TTATGATTAT GTTCACCTAC AGGGCAAGGA GGAGTTTGAG 
CGACAGCAGA AGGAGCTCTT GGAAAAGGAG AACATCATGA AGCAGAGTAA GGCGCAGCTG 
GAGCATCACC AGCTGAGTCA GTTCCAGCTG TTGGAACAAG AGATCACCAA GCCTGTGGAG 
AATGACATCT CTAAATGGAA GCCCTCTCAG AGCCTCCCAA CCACCAACAA CAGTGTGGGT 
GCTCAGGATA GGCAGTTGCT TTGCTTCTAC TATGACCAGT GCGAGACCCA TTTCATTTCC 
CTACTCAACG CCATCGACGC CCTCTTCAGC TGCGTCAGCT CAGCCCAACC CCCACGGATC 
TTTGTGGCGC ACAGCAAGTT TGTCATTCTT AGTGCGCACA AACTGGTGTT CATTGGAGAC 
ACTCTGACAA GGCAGGTGGC TGCCCAGGAC ATTCGCAACA AAGTCAGGAA CTCCAGCAAC 
CAGCTCTGCG AACAGCTCAA GACGATAGTG ATGGCGACCA AAATGGCCGC CCTCCACTAC 
CCCAGTACCA CCGCCTTGCA GGAAATGGTG CACCAGGTGA CAGACCTGTC CAGAAATGCT 
CAGCTGTTTA AGCGTTCCTT GCTGGAGATG GCCACCTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nedd9 ( NM_001111324.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001104794.1 Mus musculus neural precursor cell expressed, developmentally down-regulated gene 9 (Nedd9), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14247
Clone ID Related Accession (Same CDS sequence)	NM_001111324.2
Accession Version	NM_001111324.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	enhancer of filamentation 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY001380.1, BB842569.1, AK083374.1, AK046357.1 and AV367231.2. On Sep 24, 2013 this sequence version replaced NM_001111324.1. Transcript Variant: This variant (1) represents the longer transcript and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK083374.1, AK030985.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164140 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCGA GGAATCTTAT GGCAAGGGCC TTGTATGACA ACGTCCCTGA GTGTGCTGAG 
GAGCTGGCCT TCCGCAAGGG AGACATCTTA ACTGTCATAG AGCAGAACAC AGGAGGGCTT 
GAGGGATGGT GGCTGTGTTC CCTCCACGGT CGCCAAGGCA TTGTCCCAGG GAACCGGGTG 
AAGCTTCTGA TTGGTCCAGT GCAAGAGACC CCCGGTCATG AGCAGCCTAC TCCTGGACCT 
ATGCATCAGA CCTTTGGCCA ACAGAAACTC TATCAAGTGC CAAATTCCCA GGCAGCATCT 
CGGGATACCA TCTACCAAGT GCCACCCTCC TACCAGAATC AGGGAATTTA CCAAGTACCC 
ACTGGCCATG GCACTCCAGA ACAAGATGTA TATCAAGTAC CACCATCAGT TCAGAGGAAC 
ATTGGCGGCA CTAATGGACC CCTTCTAAGC AAAAAGGTGA TCACCCCAGT GAGGACGGGC 
CATGGCTATG TGTACGAGTA CCCATCCAGA TACCAAAAGG ATGTCTACGA TGTCCCTCCT 
TCCCACAGCA CTCAAGGGGT ATATGACATC CCTCCTTCCT CAGTAAAAGG CCCTGTGTTT 
TCAGTTCCAG TGGGAGAGAT AAAACCTCAA GGGGTATATG ACATTCCCCC CACCCAAGGG 
GTCTATGCCA TTCCACCATC GGCTTGCCGA GATGAGGCAG GGCTCAGGGA AAAGGAATAT 
GATTTCCCTC CTCCAATGAA GCAAGATGGA AAACCAGACA CCAGACCTGA GGGGGTTTAT 
GACATCCCTC CAACCAGCAC CAAGACAGCA GGCAAGGACC TTCACATCAA ATTCCCCTGT 
GATGCTCCAG GAGGTGTCGA ACCAATGGCA CGAAGACACC AGAGCTTTTC CCTGCACCAT 
GCACCCTCTC AGCTGGGACA GTCTGGGGAC ACTCAGAGTG ATGCCTATGA TGTCCCCCGG 
GGAGTTCAGT TTCTGGAGGT ACCAACAGAA ACCAGTGAAA AGGCAAATCC GGAGGAAAGA 
GACGGTGTCT ACGATGTCCC TCTGCACAAC CCAGCAGATG CCAAAGGCTC TCGGGACGTG 
GTAGATGGGA TCAACAGACT GTCTTTCTCC AGCACTGGCA GTACCAGGAG TAACATGTCC 
ACCTCTTCCA CCTCCTCAAA GGAGTCTTCA CTGTCAGCCT CCCCGTCTCA AGACAAAAGG 
CTCCGACTGG ACCCAGACAC AGCCATAGAG AAGCTCTATC GGCTCCAGCA GACCCTGGAG 
ATGGGTGTGT GCAGCCTCAT GTCACTGGTC ACCACAGACT GGAGGTGCTA CGGATACATG 
GAAAGGCACA TCAATGAGAT CCGCACCGCG GTGGACAAAG TAGAGCTGTT CTTACGAGAA 
TACCTCCATT TTGCCAAGGG AGCTTTAGCC AATGCCTCCT GCCTCCCAGA ACTGGTCCTC 
CACAACAAAA TGAAGCGGGA ACTCCAAAGA GTAGAAGATT CCCACCAGAT TCTAAGCCAA 
ACCAGCCATG ACTTGAATGA ATGCAGCTGG TCCCTGAATA TTTTAGCTAT CAATAAGCCC 
CAAAATAAGT GTGATGACCT AGACCGGTTT GTGATGGTCG CCAAGACAGT GCCAGACGAC 
GCCAAACAAC TGACCACCAC CATCAGCACC TACGCGGAGA CCCTCTTTAG AGCAGATCCT 
GCCAATTCCC ATCTGAAGAA TGGGCCCAAC AGCATCATGA ACTCAAGCGA GTACACACAT 
CCGGGCTCCC AGATGCAGCC ACTGCATCCT GGTGACTACA AAGCCCAGGT CCACAGTAAG 
CCGTTGCCTC CTAGTCTAAG CAAGGACCAG CCACCAGACT GCGGTAGCAG TGACGGTTCT 
GAGCGGAGTT GGATGGATGA TTATGATTAT GTTCACCTAC AGGGCAAGGA GGAGTTTGAG 
CGACAGCAGA AGGAGCTCTT GGAAAAGGAG AACATCATGA AGCAGAGTAA GGCGCAGCTG 
GAGCATCACC AGCTGAGTCA GTTCCAGCTG TTGGAACAAG AGATCACCAA GCCTGTGGAG 
AATGACATCT CTAAATGGAA GCCCTCTCAG AGCCTCCCAA CCACCAACAA CAGTGTGGGT 
GCTCAGGATA GGCAGTTGCT TTGCTTCTAC TATGACCAGT GCGAGACCCA TTTCATTTCC 
CTACTCAACG CCATCGACGC CCTCTTCAGC TGCGTCAGCT CAGCCCAACC CCCACGGATC 
TTTGTGGCGC ACAGCAAGTT TGTCATTCTT AGTGCGCACA AACTGGTGTT CATTGGAGAC 
ACTCTGACAA GGCAGGTGGC TGCCCAGGAC ATTCGCAACA AAGTCAGGAA CTCCAGCAAC 
CAGCTCTGCG AACAGCTCAA GACGATAGTG ATGGCGACCA AAATGGCCGC CCTCCACTAC 
CCCAGTACCA CCGCCTTGCA GGAAATGGTG CACCAGGTGA CAGACCTGTC CAGAAATGCT 
CAGCTGTTTA AGCGTTCCTT GCTGGAGATG GCCACCTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001104794.1
CDS	357..2858
Translation	MWARNLMARALYDNVPECAEELAFRKGDILTVIEQNTGGLEGWWLCSLHGRQGIVPGNRVKLLIGPVQETPGHEQPTPGPMHQTFGQQKLYQVPNSQAASRDTIYQVPPSYQNQGIYQVPTGHGTPEQDVYQVPPSVQRNIGGTNGPLLSKKVITPVRTGHGYVYEYPSRYQKDVYDVPPSHSTQGVYDIPPSSVKGPVFSVPVGEIKPQGVYDIPPTQGVYAIPPSACRDEAGLREKEYDFPPPMKQDGKPDTRPEGVYDIPPTSTKTAGKDLHIKFPCDAPGGVEPMARRHQSFSLHHAPSQLGQSGDTQSDAYDVPRGVQFLEVPTETSEKANPEERDGVYDVPLHNPADAKGSRDVVDGINRLSFSSTGSTRSNMSTSSTSSKESSLSASPSQDKRLRLDPDTAIEKLYRLQQTLEMGVCSLMSLVTTDWRCYGYMERHINEIRTAVDKVELFLREYLHFAKGALANASCLPELVLHNKMKRELQRVEDSHQILSQTSHDLNECSWSLNILAINKPQNKCDDLDRFVMVAKTVPDDAKQLTTTISTYAETLFRADPANSHLKNGPNSIMNSSEYTHPGSQMQPLHPGDYKAQVHSKPLPPSLSKDQPPDCGSSDGSERSWMDDYDYVHLQGKEEFERQQKELLEKENIMKQSKAQLEHHQLSQFQLLEQEITKPVENDISKWKPSQSLPTTNNSVGAQDRQLLCFYYDQCETHFISLLNAIDALFSCVSSAQPPRIFVAHSKFVILSAHKLVFIGDTLTRQVAAQDIRNKVRNSSNQLCEQLKTIVMATKMAALHYPSTTALQEMVHQVTDLSRNAQLFKRSLLEMATF

Target ORF information:

RefSeq Version	NM_001111324.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus neural precursor cell expressed, developmentally down-regulated gene 9 (Nedd9), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001111324.2

ORF Insert Sequence:

ATGTGGGCGA GGAATCTTAT GGCAAGGGCC TTGTATGACA ACGTCCCTGA GTGTGCTGAG 
GAGCTGGCCT TCCGCAAGGG AGACATCTTA ACTGTCATAG AGCAGAACAC AGGAGGGCTT 
GAGGGATGGT GGCTGTGTTC CCTCCACGGT CGCCAAGGCA TTGTCCCAGG GAACCGGGTG 
AAGCTTCTGA TTGGTCCAGT GCAAGAGACC CCCGGTCATG AGCAGCCTAC TCCTGGACCT 
ATGCATCAGA CCTTTGGCCA ACAGAAACTC TATCAAGTGC CAAATTCCCA GGCAGCATCT 
CGGGATACCA TCTACCAAGT GCCACCCTCC TACCAGAATC AGGGAATTTA CCAAGTACCC 
ACTGGCCATG GCACTCCAGA ACAAGATGTA TATCAAGTAC CACCATCAGT TCAGAGGAAC 
ATTGGCGGCA CTAATGGACC CCTTCTAAGC AAAAAGGTGA TCACCCCAGT GAGGACGGGC 
CATGGCTATG TGTACGAGTA CCCATCCAGA TACCAAAAGG ATGTCTACGA TGTCCCTCCT 
TCCCACAGCA CTCAAGGGGT ATATGACATC CCTCCTTCCT CAGTAAAAGG CCCTGTGTTT 
TCAGTTCCAG TGGGAGAGAT AAAACCTCAA GGGGTATATG ACATTCCCCC CACCCAAGGG 
GTCTATGCCA TTCCACCATC GGCTTGCCGA GATGAGGCAG GGCTCAGGGA AAAGGAATAT 
GATTTCCCTC CTCCAATGAA GCAAGATGGA AAACCAGACA CCAGACCTGA GGGGGTTTAT 
GACATCCCTC CAACCAGCAC CAAGACAGCA GGCAAGGACC TTCACATCAA ATTCCCCTGT 
GATGCTCCAG GAGGTGTCGA ACCAATGGCA CGAAGACACC AGAGCTTTTC CCTGCACCAT 
GCACCCTCTC AGCTGGGACA GTCTGGGGAC ACTCAGAGTG ATGCCTATGA TGTCCCCCGG 
GGAGTTCAGT TTCTGGAGGT ACCAACAGAA ACCAGTGAAA AGGCAAATCC GGAGGAAAGA 
GACGGTGTCT ACGATGTCCC TCTGCACAAC CCAGCAGATG CCAAAGGCTC TCGGGACGTG 
GTAGATGGGA TCAACAGACT GTCTTTCTCC AGCACTGGCA GTACCAGGAG TAACATGTCC 
ACCTCTTCCA CCTCCTCAAA GGAGTCTTCA CTGTCAGCCT CCCCGTCTCA AGACAAAAGG 
CTCCGACTGG ACCCAGACAC AGCCATAGAG AAGCTCTATC GGCTCCAGCA GACCCTGGAG 
ATGGGTGTGT GCAGCCTCAT GTCACTGGTC ACCACAGACT GGAGGTGCTA CGGATACATG 
GAAAGGCACA TCAATGAGAT CCGCACCGCG GTGGACAAAG TAGAGCTGTT CTTACGAGAA 
TACCTCCATT TTGCCAAGGG AGCTTTAGCC AATGCCTCCT GCCTCCCAGA ACTGGTCCTC 
CACAACAAAA TGAAGCGGGA ACTCCAAAGA GTAGAAGATT CCCACCAGAT TCTAAGCCAA 
ACCAGCCATG ACTTGAATGA ATGCAGCTGG TCCCTGAATA TTTTAGCTAT CAATAAGCCC 
CAAAATAAGT GTGATGACCT AGACCGGTTT GTGATGGTCG CCAAGACAGT GCCAGACGAC 
GCCAAACAAC TGACCACCAC CATCAGCACC TACGCGGAGA CCCTCTTTAG AGCAGATCCT 
GCCAATTCCC ATCTGAAGAA TGGGCCCAAC AGCATCATGA ACTCAAGCGA GTACACACAT 
CCGGGCTCCC AGATGCAGCC ACTGCATCCT GGTGACTACA AAGCCCAGGT CCACAGTAAG 
CCGTTGCCTC CTAGTCTAAG CAAGGACCAG CCACCAGACT GCGGTAGCAG TGACGGTTCT 
GAGCGGAGTT GGATGGATGA TTATGATTAT GTTCACCTAC AGGGCAAGGA GGAGTTTGAG 
CGACAGCAGA AGGAGCTCTT GGAAAAGGAG AACATCATGA AGCAGAGTAA GGCGCAGCTG 
GAGCATCACC AGCTGAGTCA GTTCCAGCTG TTGGAACAAG AGATCACCAA GCCTGTGGAG 
AATGACATCT CTAAATGGAA GCCCTCTCAG AGCCTCCCAA CCACCAACAA CAGTGTGGGT 
GCTCAGGATA GGCAGTTGCT TTGCTTCTAC TATGACCAGT GCGAGACCCA TTTCATTTCC 
CTACTCAACG CCATCGACGC CCTCTTCAGC TGCGTCAGCT CAGCCCAACC CCCACGGATC 
TTTGTGGCGC ACAGCAAGTT TGTCATTCTT AGTGCGCACA AACTGGTGTT CATTGGAGAC 
ACTCTGACAA GGCAGGTGGC TGCCCAGGAC ATTCGCAACA AAGTCAGGAA CTCCAGCAAC 
CAGCTCTGCG AACAGCTCAA GACGATAGTG ATGGCGACCA AAATGGCCGC CCTCCACTAC 
CCCAGTACCA CCGCCTTGCA GGAAATGGTG CACCAGGTGA CAGACCTGTC CAGAAATGCT 
CAGCTGTTTA AGCGTTCCTT GCTGGAGATG GCCACCTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nedd9 ( XM_006516874.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516937.1 Mus musculus neural precursor cell expressed, developmentally down-regulated gene 9 (Nedd9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu48520
Clone ID Related Accession (Same CDS sequence)	XM_030247179.1 , XM_006516874.3
Accession Version	XM_006516874.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	enhancer of filamentation 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516874.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAACAG TACCTAATCT TATGGCAAGG GCCTTGTATG ACAACGTCCC TGAGTGTGCT 
GAGGAGCTGG CCTTCCGCAA GGGAGACATC TTAACTGTCA TAGAGCAGAA CACAGGAGGG 
CTTGAGGGAT GGTGGCTGTG TTCCCTCCAC GGTCGCCAAG GCATTGTCCC AGGGAACCGG 
GTGAAGCTTC TGATTGGTCC AGTGCAAGAG ACCCCCGGTC ATGAGCAGCC TACTCCTGGA 
CCTATGCATC AGACCTTTGG CCAACAGAAA CTCTATCAAG TGCCAAATTC CCAGGCAGCA 
TCTCGGGATA CCATCTACCA AGTGCCACCC TCCTACCAGA ATCAGGGAAT TTACCAAGTA 
CCCACTGGCC ATGGCACTCC AGAACAAGAT GTATATCAAG TACCACCATC AGTTCAGAGG 
AACATTGGCG GCACTAATGG ACCCCTTCTA AGCAAAAAGG TGATCACCCC AGTGAGGACG 
GGCCATGGCT ATGTGTACGA GTACCCATCC AGATACCAAA AGGATGTCTA CGATGTCCCT 
CCTTCCCACA GCACTCAAGG GGTATATGAC ATCCCTCCTT CCTCAGTAAA AGGCCCTGTG 
TTTTCAGTTC CAGTGGGAGA GATAAAACCT CAAGGGGTAT ATGACATTCC CCCCACCCAA 
GGGGTCTATG CCATTCCACC ATCGGCTTGC CGAGATGAGG CAGGGCTCAG GGAAAAGGAA 
TATGATTTCC CTCCTCCAAT GAAGCAAGAT GGAAAACCAG ACACCAGACC TGAGGGGGTT 
TATGACATCC CTCCAACCAG CACCAAGACA GCAGGCAAGG ACCTTCACAT CAAATTCCCC 
TGTGATGCTC CAGGAGGTGT CGAACCAATG GCACGAAGAC ACCAGAGCTT TTCCCTGCAC 
CATGCACCCT CTCAGCTGGG ACAGTCTGGG GACACTCAGA GTGATGCCTA TGATGTCCCC 
CGGGGAGTTC AGTTTCTGGA GGTACCAACA GAAACCAGTG AAAAGGCAAA TCCGGAGGAA 
AGAGACGGTG TCTACGATGT CCCTCTGCAC AACCCAGCAG ATGCCAAAGG CTCTCGGGAC 
GTGGTAGATG GGATCAACAG ACTGTCTTTC TCCAGCACTG GCAGTACCAG GAGTAACATG 
TCCACCTCTT CCACCTCCTC AAAGGAGTCT TCACTGTCAG CCTCCCCGTC TCAAGACAAA 
AGGCTCCGAC TGGACCCAGA CACAGCCATA GAGAAGCTCT ATCGGCTCCA GCAGACCCTG 
GAGATGGGTG TGTGCAGCCT CATGTCACTG GTCACCACAG ACTGGAGGTG CTACGGATAC 
ATGGAAAGGC ACATCAATGA GATCCGCACC GCGGTGGACA AAGTAGAGCT GTTCTTACGA 
GAATACCTCC ATTTTGCCAA GGGAGCTTTA GCCAATGCCT CCTGCCTCCC AGAACTGGTC 
CTCCACAACA AAATGAAGCG GGAACTCCAA AGAGTAGAAG ATTCCCACCA GATTCTAAGC 
CAAACCAGCC ATGACTTGAA TGAATGCAGC TGGTCCCTGA ATATTTTAGC TATCAATAAG 
CCCCAAAATA AGTGTGATGA CCTAGACCGG TTTGTGATGG TCGCCAAGAC AGTGCCAGAC 
GACGCCAAAC AACTGACCAC CACCATCAGC ACCTACGCGG AGACCCTCTT TAGAGCAGAT 
CCTGCCAATT CCCATCTGAA GAATGGGCCC AACAGCATCA TGAACTCAAG CGAGTACACA 
CATCCGGGCT CCCAGATGCA GCCACTGCAT CCTGGTGACT ACAAAGCCCA GGTCCACAGT 
AAGCCGTTGC CTCCTAGTCT AAGCAAGGAC CAGCCACCAG ACTGCGGTAG CAGTGACGGT 
TCTGAGCGGA GTTGGATGGA TGATTATGAT TATGTTCACC TACAGGGCAA GGAGGAGTTT 
GAGCGACAGC AGAAGGAGCT CTTGGAAAAG GAGAACATCA TGAAGCAGAG TAAGGCGCAG 
CTGGAGCATC ACCAGCTGAG TCAGTTCCAG CTGTTGGAAC AAGAGATCAC CAAGCCTGTG 
GAGAATGACA TCTCTAAATG GAAGCCCTCT CAGAGCCTCC CAACCACCAA CAACAGTGTG 
GGTGCTCAGG ATAGGCAGTT GCTTTGCTTC TACTATGACC AGTGCGAGAC CCATTTCATT 
TCCCTACTCA ACGCCATCGA CGCCCTCTTC AGCTGCGTCA GCTCAGCCCA ACCCCCACGG 
ATCTTTGTGG CGCACAGCAA GTTTGTCATT CTTAGTGCGC ACAAACTGGT GTTCATTGGA 
GACACTCTGA CAAGGCAGGT GGCTGCCCAG GACATTCGCA ACAAAGTCAG GAACTCCAGC 
AACCAGCTCT GCGAACAGCT CAAGACGATA GTGATGGCGA CCAAAATGGC CGCCCTCCAC 
TACCCCAGTA CCACCGCCTT GCAGGAAATG GTGCACCAGG TGACAGACCT GTCCAGAAAT 
GCTCAGCTGT TTAAGCGTTC CTTGCTGGAG ATGGCCACCT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516937.1
CDS	16..2520
Translation	MPTVPNLMARALYDNVPECAEELAFRKGDILTVIEQNTGGLEGWWLCSLHGRQGIVPGNRVKLLIGPVQETPGHEQPTPGPMHQTFGQQKLYQVPNSQAASRDTIYQVPPSYQNQGIYQVPTGHGTPEQDVYQVPPSVQRNIGGTNGPLLSKKVITPVRTGHGYVYEYPSRYQKDVYDVPPSHSTQGVYDIPPSSVKGPVFSVPVGEIKPQGVYDIPPTQGVYAIPPSACRDEAGLREKEYDFPPPMKQDGKPDTRPEGVYDIPPTSTKTAGKDLHIKFPCDAPGGVEPMARRHQSFSLHHAPSQLGQSGDTQSDAYDVPRGVQFLEVPTETSEKANPEERDGVYDVPLHNPADAKGSRDVVDGINRLSFSSTGSTRSNMSTSSTSSKESSLSASPSQDKRLRLDPDTAIEKLYRLQQTLEMGVCSLMSLVTTDWRCYGYMERHINEIRTAVDKVELFLREYLHFAKGALANASCLPELVLHNKMKRELQRVEDSHQILSQTSHDLNECSWSLNILAINKPQNKCDDLDRFVMVAKTVPDDAKQLTTTISTYAETLFRADPANSHLKNGPNSIMNSSEYTHPGSQMQPLHPGDYKAQVHSKPLPPSLSKDQPPDCGSSDGSERSWMDDYDYVHLQGKEEFERQQKELLEKENIMKQSKAQLEHHQLSQFQLLEQEITKPVENDISKWKPSQSLPTTNNSVGAQDRQLLCFYYDQCETHFISLLNAIDALFSCVSSAQPPRIFVAHSKFVILSAHKLVFIGDTLTRQVAAQDIRNKVRNSSNQLCEQLKTIVMATKMAALHYPSTTALQEMVHQVTDLSRNAQLFKRSLLEMATF

Target ORF information:

RefSeq Version	XM_006516874.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neural precursor cell expressed, developmentally down-regulated gene 9 (Nedd9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516874.3

ORF Insert Sequence:

ATGCCAACAG TACCTAATCT TATGGCAAGG GCCTTGTATG ACAACGTCCC TGAGTGTGCT 
GAGGAGCTGG CCTTCCGCAA GGGAGACATC TTAACTGTCA TAGAGCAGAA CACAGGAGGG 
CTTGAGGGAT GGTGGCTGTG TTCCCTCCAC GGTCGCCAAG GCATTGTCCC AGGGAACCGG 
GTGAAGCTTC TGATTGGTCC AGTGCAAGAG ACCCCCGGTC ATGAGCAGCC TACTCCTGGA 
CCTATGCATC AGACCTTTGG CCAACAGAAA CTCTATCAAG TGCCAAATTC CCAGGCAGCA 
TCTCGGGATA CCATCTACCA AGTGCCACCC TCCTACCAGA ATCAGGGAAT TTACCAAGTA 
CCCACTGGCC ATGGCACTCC AGAACAAGAT GTATATCAAG TACCACCATC AGTTCAGAGG 
AACATTGGCG GCACTAATGG ACCCCTTCTA AGCAAAAAGG TGATCACCCC AGTGAGGACG 
GGCCATGGCT ATGTGTACGA GTACCCATCC AGATACCAAA AGGATGTCTA CGATGTCCCT 
CCTTCCCACA GCACTCAAGG GGTATATGAC ATCCCTCCTT CCTCAGTAAA AGGCCCTGTG 
TTTTCAGTTC CAGTGGGAGA GATAAAACCT CAAGGGGTAT ATGACATTCC CCCCACCCAA 
GGGGTCTATG CCATTCCACC ATCGGCTTGC CGAGATGAGG CAGGGCTCAG GGAAAAGGAA 
TATGATTTCC CTCCTCCAAT GAAGCAAGAT GGAAAACCAG ACACCAGACC TGAGGGGGTT 
TATGACATCC CTCCAACCAG CACCAAGACA GCAGGCAAGG ACCTTCACAT CAAATTCCCC 
TGTGATGCTC CAGGAGGTGT CGAACCAATG GCACGAAGAC ACCAGAGCTT TTCCCTGCAC 
CATGCACCCT CTCAGCTGGG ACAGTCTGGG GACACTCAGA GTGATGCCTA TGATGTCCCC 
CGGGGAGTTC AGTTTCTGGA GGTACCAACA GAAACCAGTG AAAAGGCAAA TCCGGAGGAA 
AGAGACGGTG TCTACGATGT CCCTCTGCAC AACCCAGCAG ATGCCAAAGG CTCTCGGGAC 
GTGGTAGATG GGATCAACAG ACTGTCTTTC TCCAGCACTG GCAGTACCAG GAGTAACATG 
TCCACCTCTT CCACCTCCTC AAAGGAGTCT TCACTGTCAG CCTCCCCGTC TCAAGACAAA 
AGGCTCCGAC TGGACCCAGA CACAGCCATA GAGAAGCTCT ATCGGCTCCA GCAGACCCTG 
GAGATGGGTG TGTGCAGCCT CATGTCACTG GTCACCACAG ACTGGAGGTG CTACGGATAC 
ATGGAAAGGC ACATCAATGA GATCCGCACC GCGGTGGACA AAGTAGAGCT GTTCTTACGA 
GAATACCTCC ATTTTGCCAA GGGAGCTTTA GCCAATGCCT CCTGCCTCCC AGAACTGGTC 
CTCCACAACA AAATGAAGCG GGAACTCCAA AGAGTAGAAG ATTCCCACCA GATTCTAAGC 
CAAACCAGCC ATGACTTGAA TGAATGCAGC TGGTCCCTGA ATATTTTAGC TATCAATAAG 
CCCCAAAATA AGTGTGATGA CCTAGACCGG TTTGTGATGG TCGCCAAGAC AGTGCCAGAC 
GACGCCAAAC AACTGACCAC CACCATCAGC ACCTACGCGG AGACCCTCTT TAGAGCAGAT 
CCTGCCAATT CCCATCTGAA GAATGGGCCC AACAGCATCA TGAACTCAAG CGAGTACACA 
CATCCGGGCT CCCAGATGCA GCCACTGCAT CCTGGTGACT ACAAAGCCCA GGTCCACAGT 
AAGCCGTTGC CTCCTAGTCT AAGCAAGGAC CAGCCACCAG ACTGCGGTAG CAGTGACGGT 
TCTGAGCGGA GTTGGATGGA TGATTATGAT TATGTTCACC TACAGGGCAA GGAGGAGTTT 
GAGCGACAGC AGAAGGAGCT CTTGGAAAAG GAGAACATCA TGAAGCAGAG TAAGGCGCAG 
CTGGAGCATC ACCAGCTGAG TCAGTTCCAG CTGTTGGAAC AAGAGATCAC CAAGCCTGTG 
GAGAATGACA TCTCTAAATG GAAGCCCTCT CAGAGCCTCC CAACCACCAA CAACAGTGTG 
GGTGCTCAGG ATAGGCAGTT GCTTTGCTTC TACTATGACC AGTGCGAGAC CCATTTCATT 
TCCCTACTCA ACGCCATCGA CGCCCTCTTC AGCTGCGTCA GCTCAGCCCA ACCCCCACGG 
ATCTTTGTGG CGCACAGCAA GTTTGTCATT CTTAGTGCGC ACAAACTGGT GTTCATTGGA 
GACACTCTGA CAAGGCAGGT GGCTGCCCAG GACATTCGCA ACAAAGTCAG GAACTCCAGC 
AACCAGCTCT GCGAACAGCT CAAGACGATA GTGATGGCGA CCAAAATGGC CGCCCTCCAC 
TACCCCAGTA CCACCGCCTT GCAGGAAATG GTGCACCAGG TGACAGACCT GTCCAGAAAT 
GCTCAGCTGT TTAAGCGTTC CTTGCTGGAG ATGGCCACCT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nedd9 ( XM_030247179.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103039.1 Mus musculus neural precursor cell expressed, developmentally down-regulated gene 9 (Nedd9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu48520
Clone ID Related Accession (Same CDS sequence)	XM_030247179.1 , XM_006516874.3
Accession Version	XM_030247179.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	enhancer of filamentation 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAACAG TACCTAATCT TATGGCAAGG GCCTTGTATG ACAACGTCCC TGAGTGTGCT 
GAGGAGCTGG CCTTCCGCAA GGGAGACATC TTAACTGTCA TAGAGCAGAA CACAGGAGGG 
CTTGAGGGAT GGTGGCTGTG TTCCCTCCAC GGTCGCCAAG GCATTGTCCC AGGGAACCGG 
GTGAAGCTTC TGATTGGTCC AGTGCAAGAG ACCCCCGGTC ATGAGCAGCC TACTCCTGGA 
CCTATGCATC AGACCTTTGG CCAACAGAAA CTCTATCAAG TGCCAAATTC CCAGGCAGCA 
TCTCGGGATA CCATCTACCA AGTGCCACCC TCCTACCAGA ATCAGGGAAT TTACCAAGTA 
CCCACTGGCC ATGGCACTCC AGAACAAGAT GTATATCAAG TACCACCATC AGTTCAGAGG 
AACATTGGCG GCACTAATGG ACCCCTTCTA AGCAAAAAGG TGATCACCCC AGTGAGGACG 
GGCCATGGCT ATGTGTACGA GTACCCATCC AGATACCAAA AGGATGTCTA CGATGTCCCT 
CCTTCCCACA GCACTCAAGG GGTATATGAC ATCCCTCCTT CCTCAGTAAA AGGCCCTGTG 
TTTTCAGTTC CAGTGGGAGA GATAAAACCT CAAGGGGTAT ATGACATTCC CCCCACCCAA 
GGGGTCTATG CCATTCCACC ATCGGCTTGC CGAGATGAGG CAGGGCTCAG GGAAAAGGAA 
TATGATTTCC CTCCTCCAAT GAAGCAAGAT GGAAAACCAG ACACCAGACC TGAGGGGGTT 
TATGACATCC CTCCAACCAG CACCAAGACA GCAGGCAAGG ACCTTCACAT CAAATTCCCC 
TGTGATGCTC CAGGAGGTGT CGAACCAATG GCACGAAGAC ACCAGAGCTT TTCCCTGCAC 
CATGCACCCT CTCAGCTGGG ACAGTCTGGG GACACTCAGA GTGATGCCTA TGATGTCCCC 
CGGGGAGTTC AGTTTCTGGA GGTACCAACA GAAACCAGTG AAAAGGCAAA TCCGGAGGAA 
AGAGACGGTG TCTACGATGT CCCTCTGCAC AACCCAGCAG ATGCCAAAGG CTCTCGGGAC 
GTGGTAGATG GGATCAACAG ACTGTCTTTC TCCAGCACTG GCAGTACCAG GAGTAACATG 
TCCACCTCTT CCACCTCCTC AAAGGAGTCT TCACTGTCAG CCTCCCCGTC TCAAGACAAA 
AGGCTCCGAC TGGACCCAGA CACAGCCATA GAGAAGCTCT ATCGGCTCCA GCAGACCCTG 
GAGATGGGTG TGTGCAGCCT CATGTCACTG GTCACCACAG ACTGGAGGTG CTACGGATAC 
ATGGAAAGGC ACATCAATGA GATCCGCACC GCGGTGGACA AAGTAGAGCT GTTCTTACGA 
GAATACCTCC ATTTTGCCAA GGGAGCTTTA GCCAATGCCT CCTGCCTCCC AGAACTGGTC 
CTCCACAACA AAATGAAGCG GGAACTCCAA AGAGTAGAAG ATTCCCACCA GATTCTAAGC 
CAAACCAGCC ATGACTTGAA TGAATGCAGC TGGTCCCTGA ATATTTTAGC TATCAATAAG 
CCCCAAAATA AGTGTGATGA CCTAGACCGG TTTGTGATGG TCGCCAAGAC AGTGCCAGAC 
GACGCCAAAC AACTGACCAC CACCATCAGC ACCTACGCGG AGACCCTCTT TAGAGCAGAT 
CCTGCCAATT CCCATCTGAA GAATGGGCCC AACAGCATCA TGAACTCAAG CGAGTACACA 
CATCCGGGCT CCCAGATGCA GCCACTGCAT CCTGGTGACT ACAAAGCCCA GGTCCACAGT 
AAGCCGTTGC CTCCTAGTCT AAGCAAGGAC CAGCCACCAG ACTGCGGTAG CAGTGACGGT 
TCTGAGCGGA GTTGGATGGA TGATTATGAT TATGTTCACC TACAGGGCAA GGAGGAGTTT 
GAGCGACAGC AGAAGGAGCT CTTGGAAAAG GAGAACATCA TGAAGCAGAG TAAGGCGCAG 
CTGGAGCATC ACCAGCTGAG TCAGTTCCAG CTGTTGGAAC AAGAGATCAC CAAGCCTGTG 
GAGAATGACA TCTCTAAATG GAAGCCCTCT CAGAGCCTCC CAACCACCAA CAACAGTGTG 
GGTGCTCAGG ATAGGCAGTT GCTTTGCTTC TACTATGACC AGTGCGAGAC CCATTTCATT 
TCCCTACTCA ACGCCATCGA CGCCCTCTTC AGCTGCGTCA GCTCAGCCCA ACCCCCACGG 
ATCTTTGTGG CGCACAGCAA GTTTGTCATT CTTAGTGCGC ACAAACTGGT GTTCATTGGA 
GACACTCTGA CAAGGCAGGT GGCTGCCCAG GACATTCGCA ACAAAGTCAG GAACTCCAGC 
AACCAGCTCT GCGAACAGCT CAAGACGATA GTGATGGCGA CCAAAATGGC CGCCCTCCAC 
TACCCCAGTA CCACCGCCTT GCAGGAAATG GTGCACCAGG TGACAGACCT GTCCAGAAAT 
GCTCAGCTGT TTAAGCGTTC CTTGCTGGAG ATGGCCACCT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103039.1
CDS	466..2970
Translation	MPTVPNLMARALYDNVPECAEELAFRKGDILTVIEQNTGGLEGWWLCSLHGRQGIVPGNRVKLLIGPVQETPGHEQPTPGPMHQTFGQQKLYQVPNSQAASRDTIYQVPPSYQNQGIYQVPTGHGTPEQDVYQVPPSVQRNIGGTNGPLLSKKVITPVRTGHGYVYEYPSRYQKDVYDVPPSHSTQGVYDIPPSSVKGPVFSVPVGEIKPQGVYDIPPTQGVYAIPPSACRDEAGLREKEYDFPPPMKQDGKPDTRPEGVYDIPPTSTKTAGKDLHIKFPCDAPGGVEPMARRHQSFSLHHAPSQLGQSGDTQSDAYDVPRGVQFLEVPTETSEKANPEERDGVYDVPLHNPADAKGSRDVVDGINRLSFSSTGSTRSNMSTSSTSSKESSLSASPSQDKRLRLDPDTAIEKLYRLQQTLEMGVCSLMSLVTTDWRCYGYMERHINEIRTAVDKVELFLREYLHFAKGALANASCLPELVLHNKMKRELQRVEDSHQILSQTSHDLNECSWSLNILAINKPQNKCDDLDRFVMVAKTVPDDAKQLTTTISTYAETLFRADPANSHLKNGPNSIMNSSEYTHPGSQMQPLHPGDYKAQVHSKPLPPSLSKDQPPDCGSSDGSERSWMDDYDYVHLQGKEEFERQQKELLEKENIMKQSKAQLEHHQLSQFQLLEQEITKPVENDISKWKPSQSLPTTNNSVGAQDRQLLCFYYDQCETHFISLLNAIDALFSCVSSAQPPRIFVAHSKFVILSAHKLVFIGDTLTRQVAAQDIRNKVRNSSNQLCEQLKTIVMATKMAALHYPSTTALQEMVHQVTDLSRNAQLFKRSLLEMATF

Target ORF information:

RefSeq Version	XM_030247179.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neural precursor cell expressed, developmentally down-regulated gene 9 (Nedd9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247179.1

ORF Insert Sequence:

ATGCCAACAG TACCTAATCT TATGGCAAGG GCCTTGTATG ACAACGTCCC TGAGTGTGCT 
GAGGAGCTGG CCTTCCGCAA GGGAGACATC TTAACTGTCA TAGAGCAGAA CACAGGAGGG 
CTTGAGGGAT GGTGGCTGTG TTCCCTCCAC GGTCGCCAAG GCATTGTCCC AGGGAACCGG 
GTGAAGCTTC TGATTGGTCC AGTGCAAGAG ACCCCCGGTC ATGAGCAGCC TACTCCTGGA 
CCTATGCATC AGACCTTTGG CCAACAGAAA CTCTATCAAG TGCCAAATTC CCAGGCAGCA 
TCTCGGGATA CCATCTACCA AGTGCCACCC TCCTACCAGA ATCAGGGAAT TTACCAAGTA 
CCCACTGGCC ATGGCACTCC AGAACAAGAT GTATATCAAG TACCACCATC AGTTCAGAGG 
AACATTGGCG GCACTAATGG ACCCCTTCTA AGCAAAAAGG TGATCACCCC AGTGAGGACG 
GGCCATGGCT ATGTGTACGA GTACCCATCC AGATACCAAA AGGATGTCTA CGATGTCCCT 
CCTTCCCACA GCACTCAAGG GGTATATGAC ATCCCTCCTT CCTCAGTAAA AGGCCCTGTG 
TTTTCAGTTC CAGTGGGAGA GATAAAACCT CAAGGGGTAT ATGACATTCC CCCCACCCAA 
GGGGTCTATG CCATTCCACC ATCGGCTTGC CGAGATGAGG CAGGGCTCAG GGAAAAGGAA 
TATGATTTCC CTCCTCCAAT GAAGCAAGAT GGAAAACCAG ACACCAGACC TGAGGGGGTT 
TATGACATCC CTCCAACCAG CACCAAGACA GCAGGCAAGG ACCTTCACAT CAAATTCCCC 
TGTGATGCTC CAGGAGGTGT CGAACCAATG GCACGAAGAC ACCAGAGCTT TTCCCTGCAC 
CATGCACCCT CTCAGCTGGG ACAGTCTGGG GACACTCAGA GTGATGCCTA TGATGTCCCC 
CGGGGAGTTC AGTTTCTGGA GGTACCAACA GAAACCAGTG AAAAGGCAAA TCCGGAGGAA 
AGAGACGGTG TCTACGATGT CCCTCTGCAC AACCCAGCAG ATGCCAAAGG CTCTCGGGAC 
GTGGTAGATG GGATCAACAG ACTGTCTTTC TCCAGCACTG GCAGTACCAG GAGTAACATG 
TCCACCTCTT CCACCTCCTC AAAGGAGTCT TCACTGTCAG CCTCCCCGTC TCAAGACAAA 
AGGCTCCGAC TGGACCCAGA CACAGCCATA GAGAAGCTCT ATCGGCTCCA GCAGACCCTG 
GAGATGGGTG TGTGCAGCCT CATGTCACTG GTCACCACAG ACTGGAGGTG CTACGGATAC 
ATGGAAAGGC ACATCAATGA GATCCGCACC GCGGTGGACA AAGTAGAGCT GTTCTTACGA 
GAATACCTCC ATTTTGCCAA GGGAGCTTTA GCCAATGCCT CCTGCCTCCC AGAACTGGTC 
CTCCACAACA AAATGAAGCG GGAACTCCAA AGAGTAGAAG ATTCCCACCA GATTCTAAGC 
CAAACCAGCC ATGACTTGAA TGAATGCAGC TGGTCCCTGA ATATTTTAGC TATCAATAAG 
CCCCAAAATA AGTGTGATGA CCTAGACCGG TTTGTGATGG TCGCCAAGAC AGTGCCAGAC 
GACGCCAAAC AACTGACCAC CACCATCAGC ACCTACGCGG AGACCCTCTT TAGAGCAGAT 
CCTGCCAATT CCCATCTGAA GAATGGGCCC AACAGCATCA TGAACTCAAG CGAGTACACA 
CATCCGGGCT CCCAGATGCA GCCACTGCAT CCTGGTGACT ACAAAGCCCA GGTCCACAGT 
AAGCCGTTGC CTCCTAGTCT AAGCAAGGAC CAGCCACCAG ACTGCGGTAG CAGTGACGGT 
TCTGAGCGGA GTTGGATGGA TGATTATGAT TATGTTCACC TACAGGGCAA GGAGGAGTTT 
GAGCGACAGC AGAAGGAGCT CTTGGAAAAG GAGAACATCA TGAAGCAGAG TAAGGCGCAG 
CTGGAGCATC ACCAGCTGAG TCAGTTCCAG CTGTTGGAAC AAGAGATCAC CAAGCCTGTG 
GAGAATGACA TCTCTAAATG GAAGCCCTCT CAGAGCCTCC CAACCACCAA CAACAGTGTG 
GGTGCTCAGG ATAGGCAGTT GCTTTGCTTC TACTATGACC AGTGCGAGAC CCATTTCATT 
TCCCTACTCA ACGCCATCGA CGCCCTCTTC AGCTGCGTCA GCTCAGCCCA ACCCCCACGG 
ATCTTTGTGG CGCACAGCAA GTTTGTCATT CTTAGTGCGC ACAAACTGGT GTTCATTGGA 
GACACTCTGA CAAGGCAGGT GGCTGCCCAG GACATTCGCA ACAAAGTCAG GAACTCCAGC 
AACCAGCTCT GCGAACAGCT CAAGACGATA GTGATGGCGA CCAAAATGGC CGCCCTCCAC 
TACCCCAGTA CCACCGCCTT GCAGGAAATG GTGCACCAGG TGACAGACCT GTCCAGAAAT 
GCTCAGCTGT TTAAGCGTTC CTTGCTGGAG ATGGCCACCT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Actin polymerization-dependent activation of Cas-L promotes immunological synapse stability.
Immunology and cell biology94(10)981-993(2016 11)
Santos LC,Blair DA,Kumari S,Cammer M,Iskratsch T,Herbin O,Alexandropoulos K,Dustin ML,Sheetz MP

Mice null for NEDD9 (HEF1?) display extensive hippocampal dendritic spine loss and cognitive impairment.
Brain research1632141-55(2016 Feb)
Knutson DC,Mitzey AM,Talton LE,Clagett-Dame M

Identification of Nedd9 as a TGF-?-Smad2/3 Target Gene Involved in RANKL-Induced Osteoclastogenesis by Comprehensive Analysis.
PloS one11(6)e0157992(2016)
Omata Y,Nakamura S,Koyama T,Yasui T,Hirose J,Izawa N,Matsumoto T,Imai Y,Seo S,Kurokawa M,Tsutsumi S,Kadono Y,Morimoto C,Aburatani H,Miyamoto T,Tanaka S

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Nedd9 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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