Mgp cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mgp
Entrez Gene ID	17313
Full Name	matrix Gla protein
Synonyms	Mglap
General protein information	Preferred Names matrix Gla protein Names matrix Gla protein matrix gamma-carboxyglutamate (gla) protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 G1
Summary	This gene encodes a member of the osteocalcin/matrix Gla family of proteins. The encoded vitamin K-dependent protein is secreted by chondrocytes and vascular smooth muscle cells, and functions as a physiological inhibitor of ectopic tissue calcification. This protein also inhibits angiogenesis. Mice lacking a functional copy of this gene exhibit impaired differentiation of endothelial cells, reduced stature, and calcification and rupture of the vasculature leading to premature death. [provided by RefSeq, Sep 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_008597.4	NP_032623.1	matrix Gla protein precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	MGP	NP_000891.2
	MGP	XP_001090877.1
Canis lupus familiaris (dog)	MGP	XP_003640024.1
Bos taurus (cattle)	MGP	NP_777132.1
Mus musculus (house mouse)	Mgp	NP_032623.1
Pan troglodytes (chimpanzee)	MGP	XP_003952171.1
Rattus norvegicus (Norway rat)	Mgp	NP_036994.1

Gene Ontology
Bibliography

Related articles in PubMed

Vascular endothelium plays a key role in directing pulmonary epithelial cell differentiation.
Yao J, Guihard PJ, Wu X, Blazquez-Medela AM, Spencer MJ, Jumabay M, Tontonoz P, Fogelman AM, Bostr?m KI, Yao Y
The Journal of cell biology216(10)3369-3385(2017 Oct)

Matrix Gla protein deficiency impairs nasal septum growth, causing midface hypoplasia.
Marulanda J, Eimar H, McKee MD, Berkvens M, Nelea V, Roman H, Borr?s T, Tamimi F, Ferron M, Murshed M
The Journal of biological chemistry292(27)11400-11412(2017 07)

A single gene connects stiffness in glaucoma and the vascular system.
Borr?s T
Experimental eye research15813-22(2017 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the nasal septum to be abnormally mineralized and shortened in Mgp(-/-) mice. Transgenic restoration of Mgp expression in chondrocytes fully corrected the craniofacial anomalies caused by MGP deficiency, suggesting a local role for MGP in the developing nasal septum.
Title: Matrix Gla protein deficiency impairs nasal septum growth, causing midface hypoplasia.

We propose MGP as a strong candidate for the regulation of stiffness in glaucoma. MGP further illustrates the presence of a common function affecting key glaucomatous parameters in the front and back of the eye, and thus offers the possibility for a sole therapeutic target for the disease.
Title: A single gene connects stiffness in glaucoma and the vascular system.

loss of MGP causes dysregulation of early endothelial differentiation.
Title: Matrix Gla protein regulates differentiation of endothelial cells derived from mouse embryonic stem cells.

Matrix Gla's restricted expression to glaucoma-associated tissues from anterior and posterior segments suggests its involvement in the development of the disease.
Title: A Novel Mgp-Cre Knock-In Mouse Reveals an Anticalcification/Antistiffness Candidate Gene in the Trabecular Meshwork and Peripapillary Scleral Region.

Matrix Gla protein limits pulmonary arteriovenous malformations in ALK1 deficiency.
Title: Matrix Gla protein limits pulmonary arteriovenous malformations in ALK1 deficiency.

The following Mgp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mgp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18706	NM_008597.4 Latest version!	Mus musculus matrix Gla protein (Mgp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mgp ( NM_008597.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032623.1 Mus musculus matrix Gla protein (Mgp), mRNA.

Price & Availability↑

CloneID	OMu18706
Clone ID Related Accession (Same CDS sequence)	NM_008597.4
Accession Version	NM_008597.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	matrix Gla protein precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC122804.4, AK018719.1 and BY702829.1. On Sep 21, 2016 this sequence version replaced NM_008597.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BY702829.1, EL964633.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGAGCC TGCTCCCTCT GGCCATCCTG GCTGCGCTGG CCGTGGCAAC CCTGTGCTAC 
GAATCTCACG AAAGCATGGA GTCCTATGAA ATCAGTCCCT TCATCAACAG GAGAAATGCC 
AACACCTTTA TGTCCCCTCA GCAGAGGTGG CGAGCTAAAG CCCAAAAGAG AGTCCAGGAA 
CGCAACAAGC CTGCCTACGA GATCAACAGA GAGGCCTGCG ATGACTACAA GCTGTGTGAG 
CGCTACGCCA TGGTCTACGG CTACAACGCT GCCTACAACC GCTACTTCAG GCAGCGCCGA 
GGAGCCAAAT ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032623.1
CDS	94..408
Translation	MKSLLPLAILAALAVATLCYESHESMESYEISPFINRRNANTFMSPQQRWRAKAQKRVQERNKPAYEINREACDDYKLCERYAMVYGYNAAYNRYFRQRRGAKY

Target ORF information:

RefSeq Version	NM_008597.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus matrix Gla protein (Mgp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008597.4

ORF Insert Sequence:

ATGAAGAGCC TGCTCCCTCT GGCCATCCTG GCTGCGCTGG CCGTGGCAAC CCTGTGCTAC 
GAATCTCACG AAAGCATGGA GTCCTATGAA ATCAGTCCCT TCATCAACAG GAGAAATGCC 
AACACCTTTA TGTCCCCTCA GCAGAGGTGG CGAGCTAAAG CCCAAAAGAG AGTCCAGGAA 
CGCAACAAGC CTGCCTACGA GATCAACAGA GAGGCCTGCG ATGACTACAA GCTGTGTGAG 
CGCTACGCCA TGGTCTACGG CTACAACGCT GCCTACAACC GCTACTTCAG GCAGCGCCGA 
GGAGCCAAAT ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Vascular endothelium plays a key role in directing pulmonary epithelial cell differentiation.
The Journal of cell biology216(10)3369-3385(2017 Oct)
Yao J,Guihard PJ,Wu X,Blazquez-Medela AM,Spencer MJ,Jumabay M,Tontonoz P,Fogelman AM,Bostr?m KI,Yao Y

Matrix Gla protein deficiency impairs nasal septum growth, causing midface hypoplasia.
The Journal of biological chemistry292(27)11400-11412(2017 07)
Marulanda J,Eimar H,McKee MD,Berkvens M,Nelea V,Roman H,Borr?s T,Tamimi F,Ferron M,Murshed M

A single gene connects stiffness in glaucoma and the vascular system.
Experimental eye research15813-22(2017 05)
Borr?s T