Gzmk cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gzmk
Entrez Gene ID	14945
Full Name	granzyme K
General protein information	Preferred Names granzyme K Names granzyme K
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 D2.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_008196.1	NP_032222.1	granzyme K precursor
NM_008196.2	NP_032222.1	granzyme K precursor

Pathways from BioSystems

Homologies

	GZMK	XP_001097338.1
Rattus norvegicus (Norway rat)	Gzmk	NP_058815.1
Homo sapiens (human)	GZMK	NP_002095.1
Bos taurus (cattle)	GZMK	NP_001192888.1
Danio rerio (zebrafish)	gzmk	XP_001335076.2
Pan troglodytes (chimpanzee)	GZMK	XP_527196.1
Canis lupus familiaris (dog)	GZMK	XP_546318.2
Mus musculus (house mouse)	Gzmk	NP_032222.1
Danio rerio (zebrafish)	gzma	XP_001335166.1

Gene Ontology
Bibliography

Related articles in PubMed

Granzyme K-deficient mice show no evidence of impaired antiviral immunity.
Joeckel LT, Allison CC, Pellegrini M, Bird CH, Bird PI
Immunology and cell biology95(8)676-683(2017 Sep)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Substrate specificities of the granzyme tryptases A and K.
Plasman K, Demol H, Bird PI, Gevaert K, Van Damme P
Journal of proteome research13(12)6067-77(2014 Dec)

Granzyme K synergistically potentiates LPS-induced cytokine responses in human monocytes.
Wensink AC, Kemp V, Fermie J, Garc?a Laorden MI, van der Poll T, Hack CE, Bovenschen N
Proceedings of the National Academy of Sciences of the United States of America111(16)5974-9(2014 Apr)

Granzyme K inhibits replication of influenza virus through cleaving the nuclear transport complex importin ?1/? dimer of infected host cells.
Zhong C, Li C, Wang X, Toyoda T, Gao G, Fan Z
Cell death and differentiation19(5)882-90(2012 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate N-terminomics on the human and mouse granzymes A and K by combined fractional diagonal chromatography (COFRADIC).
Title: Substrate specificities of the granzyme tryptases A and K.

The data suggest that T-killer cells control lymphocytic choriomeningitis virus through non-cytotoxic processes that involve gzmK.
Title: Mouse granzyme K has pro-inflammatory potential.

It was suggested that a high predisposition to catalepsy in mice can be defined by the Map3k1, Il6st, Gzmk, and Hspb3 genes' coexpression network.
Title: Map3k1, Il6st, Gzmk, and Hspb3 gene coexpression network in the mechanism of freezing reaction in mice.

grzK plays an important role in CD8(+) T-cell cytotoxicity both in the presence and absence of grzA and B.
Title: Granzyme K expressing cytotoxic T lymphocytes protects against influenza virus in granzyme AB-/- mice.

GzmK-induced caspase-independent death occurs through Bid-dependent mitochondrial damage that is different from GzmA
Title: Granzyme K directly processes bid to release cytochrome c and endonuclease G leading to mitochondria-dependent cell death.

The following Gzmk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gzmk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01511	NM_008196.2 Latest version!	Mus musculus granzyme K (Gzmk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
View Old Accession Versions >>
OMu01511	NM_008196.1	Mus musculus granzyme K (Gzmk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gzmk ( NM_008196.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032222.1 Mus musculus granzyme K (Gzmk), mRNA.

Price & Availability↑

CloneID	OMu01511
Clone ID Related Accession (Same CDS sequence)	NM_008196.1 , NM_008196.2
Accession Version	NM_008196.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Mus musculus(house mouse)
Product	granzyme K precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF011446.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC117981.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGAGGTTTT CTTCATGGGC TCTGGTTTCC CTGGTGGCTG GCGTTTATAT GTCTTCAGAG 
TGTTTCCATA CTGAAATTAT TGGAGGGAGG GAAGTCCAGC CGCATTCCAG GCCATTTATG 
GCGTCCATCC AGTACCGCAG CAAGCATATT TGTGGAGGAG TCCTGATCCA CCCACAGTGG 
GTGCTAACAG CCGCCCACTG CTACTCTTGG TTTCCCAGAG GCCACTCTCC CACCGTGGTT 
TTAGGAGCAC ATTCTCTTTC CAAGAATGAG CCCATGAAGC AGACATTTGA AATTAAAAAG 
TTCATCCCAT TCTCACGACT TCAGTCCGGT TCCGCATCGC ATGACATCAT GCTGATAAAG 
CTTCGCACTG CTGCAGAACT AAACAAGAAT GTCCAACTGC TTCACCTGGG ATCCAAAAAC 
TATCTTAGAG ATGGGACCAA ATGCCAGGTG ACTGGCTGGG GAACCACCAA GCCAGATCTG 
TTAACCGCCT CTGATACCCT GAGAGAAGTC ACTGTTACCA TCATAAGTAG AAAACGCTGT 
AACAGCCAAA GCTACTACAA CCACAAACCT GTTATAACCA AGGACATGAT ATGTGCAGGA 
GATGCCAGAG GTCAAAAGGA TTCCTGCAAG GGTGACTCTG GTGGCCCTTT GATCTGCAAA 
GGCATCTTCC ATGCCCTAGT CTCTCAGGGC TATAAATGTG GCATCGCCAA AAAGCCTGGA 
ATCTATACGC TATTAACTAA GAAATACCAG ACCTGGATCA AAAGCAAGCT TGCCCCATCA 
CGTGCACATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032222.1
CDS	1..792
Translation	MRFSSWALVSLVAGVYMSSECFHTEIIGGREVQPHSRPFMASIQYRSKHICGGVLIHPQWVLTAAHCYSWFPRGHSPTVVLGAHSLSKNEPMKQTFEIKKFIPFSRLQSGSASHDIMLIKLRTAAELNKNVQLLHLGSKNYLRDGTKCQVTGWGTTKPDLLTASDTLREVTVTIISRKRCNSQSYYNHKPVITKDMICAGDARGQKDSCKGDSGGPLICKGIFHALVSQGYKCGIAKKPGIYTLLTKKYQTWIKSKLAPSRAH

Target ORF information:

RefSeq Version	NM_008196.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus granzyme K (Gzmk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008196.1

ORF Insert Sequence:

ATGAGGTTTT CTTCATGGGC TCTGGTTTCC CTGGTGGCTG GCGTTTATAT GTCTTCAGAG 
TGTTTCCATA CTGAAATTAT TGGAGGGAGG GAAGTCCAGC CGCATTCCAG GCCATTTATG 
GCGTCCATCC AGTACCGCAG CAAGCATATT TGTGGAGGAG TCCTGATCCA CCCACAGTGG 
GTGCTAACAG CCGCCCACTG CTACTCTTGG TTTCCCAGAG GCCACTCTCC CACCGTGGTT 
TTAGGAGCAC ATTCTCTTTC CAAGAATGAG CCCATGAAGC AGACATTTGA AATTAAAAAG 
TTCATCCCAT TCTCACGACT TCAGTCCGGT TCCGCATCGC ATGACATCAT GCTGATAAAG 
CTTCGCACTG CTGCAGAACT AAACAAGAAT GTCCAACTGC TTCACCTGGG ATCCAAAAAC 
TATCTTAGAG ATGGGACCAA ATGCCAGGTG ACTGGCTGGG GAACCACCAA GCCAGATCTG 
TTAACCGCCT CTGATACCCT GAGAGAAGTC ACTGTTACCA TCATAAGTAG AAAACGCTGT 
AACAGCCAAA GCTACTACAA CCACAAACCT GTTATAACCA AGGACATGAT ATGTGCAGGA 
GATGCCAGAG GTCAAAAGGA TTCCTGCAAG GGTGACTCTG GTGGCCCTTT GATCTGCAAA 
GGCATCTTCC ATGCCCTAGT CTCTCAGGGC TATAAATGTG GCATCGCCAA AAAGCCTGGA 
ATCTATACGC TATTAACTAA GAAATACCAG ACCTGGATCA AAAGCAAGCT TGCCCCATCA 
CGTGCACATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gzmk ( NM_008196.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032222.1 Mus musculus granzyme K (Gzmk), mRNA.

Price & Availability↑

CloneID	OMu01511
Clone ID Related Accession (Same CDS sequence)	NM_008196.1 , NM_008196.2
Accession Version	NM_008196.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Mus musculus(house mouse)
Product	granzyme K precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC139155.3. On Feb 4, 2019 this sequence version replaced NM_008196.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189685.235253.1, SRR5189676.4614.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGAGGTTTT CTTCATGGGC TCTGGTTTCC CTGGTGGCTG GCGTTTATAT GTCTTCAGAG 
TGTTTCCATA CTGAAATTAT TGGAGGGAGG GAAGTCCAGC CGCATTCCAG GCCATTTATG 
GCGTCCATCC AGTACCGCAG CAAGCATATT TGTGGAGGAG TCCTGATCCA CCCACAGTGG 
GTGCTAACAG CCGCCCACTG CTACTCTTGG TTTCCCAGAG GCCACTCTCC CACCGTGGTT 
TTAGGAGCAC ATTCTCTTTC CAAGAATGAG CCCATGAAGC AGACATTTGA AATTAAAAAG 
TTCATCCCAT TCTCACGACT TCAGTCCGGT TCCGCATCGC ATGACATCAT GCTGATAAAG 
CTTCGCACTG CTGCAGAACT AAACAAGAAT GTCCAACTGC TTCACCTGGG ATCCAAAAAC 
TATCTTAGAG ATGGGACCAA ATGCCAGGTG ACTGGCTGGG GAACCACCAA GCCAGATCTG 
TTAACCGCCT CTGATACCCT GAGAGAAGTC ACTGTTACCA TCATAAGTAG AAAACGCTGT 
AACAGCCAAA GCTACTACAA CCACAAACCT GTTATAACCA AGGACATGAT ATGTGCAGGA 
GATGCCAGAG GTCAAAAGGA TTCCTGCAAG GGTGACTCTG GTGGCCCTTT GATCTGCAAA 
GGCATCTTCC ATGCCCTAGT CTCTCAGGGC TATAAATGTG GCATCGCCAA AAAGCCTGGA 
ATCTATACGC TATTAACTAA GAAATACCAG ACCTGGATCA AAAGCAAGCT TGCCCCATCA 
CGTGCACATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032222.1
CDS	69..860
Translation	MRFSSWALVSLVAGVYMSSECFHTEIIGGREVQPHSRPFMASIQYRSKHICGGVLIHPQWVLTAAHCYSWFPRGHSPTVVLGAHSLSKNEPMKQTFEIKKFIPFSRLQSGSASHDIMLIKLRTAAELNKNVQLLHLGSKNYLRDGTKCQVTGWGTTKPDLLTASDTLREVTVTIISRKRCNSQSYYNHKPVITKDMICAGDARGQKDSCKGDSGGPLICKGIFHALVSQGYKCGIAKKPGIYTLLTKKYQTWIKSKLAPSRAH

Target ORF information:

RefSeq Version	NM_008196.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus granzyme K (Gzmk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008196.2

ORF Insert Sequence:

ATGAGGTTTT CTTCATGGGC TCTGGTTTCC CTGGTGGCTG GCGTTTATAT GTCTTCAGAG 
TGTTTCCATA CTGAAATTAT TGGAGGGAGG GAAGTCCAGC CGCATTCCAG GCCATTTATG 
GCGTCCATCC AGTACCGCAG CAAGCATATT TGTGGAGGAG TCCTGATCCA CCCACAGTGG 
GTGCTAACAG CCGCCCACTG CTACTCTTGG TTTCCCAGAG GCCACTCTCC CACCGTGGTT 
TTAGGAGCAC ATTCTCTTTC CAAGAATGAG CCCATGAAGC AGACATTTGA AATTAAAAAG 
TTCATCCCAT TCTCACGACT TCAGTCCGGT TCCGCATCGC ATGACATCAT GCTGATAAAG 
CTTCGCACTG CTGCAGAACT AAACAAGAAT GTCCAACTGC TTCACCTGGG ATCCAAAAAC 
TATCTTAGAG ATGGGACCAA ATGCCAGGTG ACTGGCTGGG GAACCACCAA GCCAGATCTG 
TTAACCGCCT CTGATACCCT GAGAGAAGTC ACTGTTACCA TCATAAGTAG AAAACGCTGT 
AACAGCCAAA GCTACTACAA CCACAAACCT GTTATAACCA AGGACATGAT ATGTGCAGGA 
GATGCCAGAG GTCAAAAGGA TTCCTGCAAG GGTGACTCTG GTGGCCCTTT GATCTGCAAA 
GGCATCTTCC ATGCCCTAGT CTCTCAGGGC TATAAATGTG GCATCGCCAA AAAGCCTGGA 
ATCTATACGC TATTAACTAA GAAATACCAG ACCTGGATCA AAAGCAAGCT TGCCCCATCA 
CGTGCACATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Granzyme K-deficient mice show no evidence of impaired antiviral immunity.
Immunology and cell biology95(8)676-683(2017 Sep)
Joeckel LT,Allison CC,Pellegrini M,Bird CH,Bird PI

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Substrate specificities of the granzyme tryptases A and K.
Journal of proteome research13(12)6067-77(2014 Dec)
Plasman K,Demol H,Bird PI,Gevaert K,Van Damme P

Granzyme K synergistically potentiates LPS-induced cytokine responses in human monocytes.
Proceedings of the National Academy of Sciences of the United States of America111(16)5974-9(2014 Apr)
Wensink AC,Kemp V,Fermie J,Garc?a Laorden MI,van der Poll T,Hack CE,Bovenschen N

Granzyme K inhibits replication of influenza virus through cleaving the nuclear transport complex importin ?1/? dimer of infected host cells.
Cell death and differentiation19(5)882-90(2012 May)
Zhong C,Li C,Wang X,Toyoda T,Gao G,Fan Z

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Gzmk cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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