GZMK cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GZMK
Entrez Gene ID	3003
Full Name	granzyme K
Synonyms	TRYP2
General protein information	Preferred Names granzyme K Names granzyme K NK-Tryp-2 NK-tryptase-2 fragmentin-3 granzyme 3 granzyme K (granzyme 3; tryptase II) granzyme K (serine protease, granzyme 3; tryptase II) tryptase II
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q11.2
Summary	This gene product is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. [provided by RefSeq, Jul 2008].
Disorder MIM:	600784

mRNA and Protein(s)

mRNA	Protein	Name
NM_002104.3	NP_002095.1	granzyme K precursor
NM_002104.2	NP_002095.1	granzyme K precursor

Pathways from BioSystems

Homologies

	GZMK	XP_001097338.1
Rattus norvegicus (Norway rat)	Gzmk	NP_058815.1
Homo sapiens (human)	GZMK	NP_002095.1
Bos taurus (cattle)	GZMK	NP_001192888.1
Danio rerio (zebrafish)	gzmk	XP_001335076.2
Pan troglodytes (chimpanzee)	GZMK	XP_527196.1
Canis lupus familiaris (dog)	GZMK	XP_546318.2
Mus musculus (house mouse)	Gzmk	NP_032222.1
Danio rerio (zebrafish)	gzma	XP_001335166.1

Gene Ontology
Bibliography

Related articles in PubMed

Extracellular granzyme K mediates endothelial activation through the cleavage of protease-activated receptor-1.
Sharma M, Merkulova Y, Raithatha S, Parkinson LG, Shen Y, Cooper D, Granville DJ
The FEBS journal283(9)1734-47(2016 05)

Effect of carbamate pesticides on perforin, granzymes A-B-3/K, and granulysin in human natural killer cells.
Li Q, Kobayashi M, Kawada T
International journal of immunopathology and pharmacology28(3)403-10(2015 Sep)

Substrate specificities of the granzyme tryptases A and K.
Plasman K, Demol H, Bird PI, Gevaert K, Van Damme P
Journal of proteome research13(12)6067-77(2014 Dec)

Granzyme K synergistically potentiates LPS-induced cytokine responses in human monocytes.
Wensink AC, Kemp V, Fermie J, Garc?a Laorden MI, van der Poll T, Hack CE, Bovenschen N
Proceedings of the National Academy of Sciences of the United States of America111(16)5974-9(2014 Apr)

Unexpected role for granzyme K in CD56bright NK cell-mediated immunoregulation of multiple sclerosis.
Jiang W, Chai NR, Maric D, Bielekova B
Journal of immunology (Baltimore, Md. : 1950)187(2)781-90(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Extracellular granzyme K mediates endothelial activation through the cleavage of PAR-1.
Title: Extracellular granzyme K mediates endothelial activation through the cleavage of protease-activated receptor-1.

Carbamate pesticides significantly reduced the intracellular levels of perforin, GrA, GrB, Gr3/K, and GRN in NK-92CI cells.
Title: Effect of carbamate pesticides on perforin, granzymes A-B-3/K, and granulysin in human natural killer cells.

Data indicate N-terminomics on the human and mouse granzymes A and K by combined fractional diagonal chromatography (COFRADIC).
Title: Substrate specificities of the granzyme tryptases A and K.

Extracellular GrK is an unexpected direct modulator of lipopolysaccharide-TLR4 signaling during the antimicrobial innate immune response.
Title: Granzyme K synergistically potentiates LPS-induced cytokine responses in human monocytes.

Granzyme K plays an important physiological role in immunoregulation of adaptive immunity underlying the cytotoxicity of CD56(bright) natural killer (NK) cells toward activated T cells.
Title: Unexpected role for granzyme K in CD56bright NK cell-mediated immunoregulation of multiple sclerosis.

The following GZMK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GZMK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30001	NM_002104.3 Latest version!	Homo sapiens granzyme K (GZMK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu30001	NM_002104.2	Homo sapiens granzyme K (GZMK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GZMK ( NM_002104.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002095.1 Homo sapiens granzyme K (GZMK), mRNA.

Price & Availability↑

CloneID	OHu30001
Clone ID Related Accession (Same CDS sequence)	NM_002104.3 , NM_002104.2
Accession Version	NM_002104.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	granzyme K precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC034238.4 and BC035802.1. On May 17, 2019 this sequence version replaced NM_002104.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189667.268483.1, AK312074.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000231009.3/ ENSP00000231009.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACTAAGT TTTCTTCCTT TTCTCTGTTT TTCCTAATAG TTGGGGCTTA TATGACTCAT 
GTGTGTTTCA ATATGGAAAT TATTGGAGGG AAAGAAGTGT CACCTCATTC CAGGCCATTT 
ATGGCCTCCA TCCAGTATGG CGGACATCAC GTTTGTGGAG GTGTTCTGAT TGATCCACAG 
TGGGTGCTGA CAGCAGCCCA CTGCCAATAT CGGTTTACCA AAGGCCAGTC TCCCACTGTG 
GTTTTAGGCG CACACTCTCT CTCAAAGAAT GAGGCCTCCA AACAAACACT GGAGATCAAA 
AAATTTATAC CATTCTCAAG AGTTACATCA GATCCTCAAT CAAATGATAT CATGCTGGTT 
AAGCTTCAAA CAGCCGCAAA ACTCAATAAA CATGTCAAGA TGCTCCACAT AAGATCCAAA 
ACCTCTCTTA GATCTGGAAC CAAATGCAAG GTTACTGGCT GGGGAGCCAC CGATCCAGAT 
TCATTAAGAC CTTCTGACAC CCTGCGAGAA GTCACTGTTA CTGTCCTAAG TCGAAAACTT 
TGCAACAGCC AAAGTTACTA CAACGGCGAC CCTTTTATCA CCAAAGACAT GGTCTGTGCA 
GGAGATGCCA AAGGCCAGAA GGATTCCTGT AAGGGTGACT CAGGGGGCCC CTTGATCTGT 
AAAGGTGTCT TCCACGCTAT AGTCTCTGGA GGTCATGAAT GTGGTGTTGC CACAAAGCCT 
GGAATCTACA CCCTGTTAAC CAAGAAATAC CAGACTTGGA TCAAAAGCAA CCTTGTCCCG 
CCTCATACAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002095.1
CDS	68..862
Translation	MTKFSSFSLFFLIVGAYMTHVCFNMEIIGGKEVSPHSRPFMASIQYGGHHVCGGVLIDPQWVLTAAHCQYRFTKGQSPTVVLGAHSLSKNEASKQTLEIKKFIPFSRVTSDPQSNDIMLVKLQTAAKLNKHVKMLHIRSKTSLRSGTKCKVTGWGATDPDSLRPSDTLREVTVTVLSRKLCNSQSYYNGDPFITKDMVCAGDAKGQKDSCKGDSGGPLICKGVFHAIVSGGHECGVATKPGIYTLLTKKYQTWIKSNLVPPHTN

Target ORF information:

RefSeq Version	NM_002104.3
Organism	Homo sapiens(human)
Definition	Homo sapiens granzyme K (GZMK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002104.3

ORF Insert Sequence:

ATGACTAAGT TTTCTTCCTT TTCTCTGTTT TTCCTAATAG TTGGGGCTTA TATGACTCAT 
GTGTGTTTCA ATATGGAAAT TATTGGAGGG AAAGAAGTGT CACCTCATTC CAGGCCATTT 
ATGGCCTCCA TCCAGTATGG CGGACATCAC GTTTGTGGAG GTGTTCTGAT TGATCCACAG 
TGGGTGCTGA CAGCAGCCCA CTGCCAATAT CGGTTTACCA AAGGCCAGTC TCCCACTGTG 
GTTTTAGGCG CACACTCTCT CTCAAAGAAT GAGGCCTCCA AACAAACACT GGAGATCAAA 
AAATTTATAC CATTCTCAAG AGTTACATCA GATCCTCAAT CAAATGATAT CATGCTGGTT 
AAGCTTCAAA CAGCCGCAAA ACTCAATAAA CATGTCAAGA TGCTCCACAT AAGATCCAAA 
ACCTCTCTTA GATCTGGAAC CAAATGCAAG GTTACTGGCT GGGGAGCCAC CGATCCAGAT 
TCATTAAGAC CTTCTGACAC CCTGCGAGAA GTCACTGTTA CTGTCCTAAG TCGAAAACTT 
TGCAACAGCC AAAGTTACTA CAACGGCGAC CCTTTTATCA CCAAAGACAT GGTCTGTGCA 
GGAGATGCCA AAGGCCAGAA GGATTCCTGT AAGGGTGACT CAGGGGGCCC CTTGATCTGT 
AAAGGTGTCT TCCACGCTAT AGTCTCTGGA GGTCATGAAT GTGGTGTTGC CACAAAGCCT 
GGAATCTACA CCCTGTTAAC CAAGAAATAC CAGACTTGGA TCAAAAGCAA CCTTGTCCCG 
CCTCATACAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GZMK ( NM_002104.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_002095.1 Homo sapiens granzyme K (GZMK), mRNA.

Price & Availability↑

CloneID	OHu30001
Clone ID Related Accession (Same CDS sequence)	NM_002104.3 , NM_002104.2
Accession Version	NM_002104.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	granzyme K precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC035802.1. On Aug 24, 2005 this sequence version replaced NM_002104.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC035802.1, U26174.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTAAGT TTTCTTCCTT TTCTCTGTTT TTCCTAATAG TTGGGGCTTA TATGACTCAT 
GTGTGTTTCA ATATGGAAAT TATTGGAGGG AAAGAAGTGT CACCTCATTC CAGGCCATTT 
ATGGCCTCCA TCCAGTATGG CGGACATCAC GTTTGTGGAG GTGTTCTGAT TGATCCACAG 
TGGGTGCTGA CAGCAGCCCA CTGCCAATAT CGGTTTACCA AAGGCCAGTC TCCCACTGTG 
GTTTTAGGCG CACACTCTCT CTCAAAGAAT GAGGCCTCCA AACAAACACT GGAGATCAAA 
AAATTTATAC CATTCTCAAG AGTTACATCA GATCCTCAAT CAAATGATAT CATGCTGGTT 
AAGCTTCAAA CAGCCGCAAA ACTCAATAAA CATGTCAAGA TGCTCCACAT AAGATCCAAA 
ACCTCTCTTA GATCTGGAAC CAAATGCAAG GTTACTGGCT GGGGAGCCAC CGATCCAGAT 
TCATTAAGAC CTTCTGACAC CCTGCGAGAA GTCACTGTTA CTGTCCTAAG TCGAAAACTT 
TGCAACAGCC AAAGTTACTA CAACGGCGAC CCTTTTATCA CCAAAGACAT GGTCTGTGCA 
GGAGATGCCA AAGGCCAGAA GGATTCCTGT AAGGGTGACT CAGGGGGCCC CTTGATCTGT 
AAAGGTGTCT TCCACGCTAT AGTCTCTGGA GGTCATGAAT GTGGTGTTGC CACAAAGCCT 
GGAATCTACA CCCTGTTAAC CAAGAAATAC CAGACTTGGA TCAAAAGCAA CCTTGTCCCG 
CCTCATACAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002095.1
CDS	45..839
Translation	MTKFSSFSLFFLIVGAYMTHVCFNMEIIGGKEVSPHSRPFMASIQYGGHHVCGGVLIDPQWVLTAAHCQYRFTKGQSPTVVLGAHSLSKNEASKQTLEIKKFIPFSRVTSDPQSNDIMLVKLQTAAKLNKHVKMLHIRSKTSLRSGTKCKVTGWGATDPDSLRPSDTLREVTVTVLSRKLCNSQSYYNGDPFITKDMVCAGDAKGQKDSCKGDSGGPLICKGVFHAIVSGGHECGVATKPGIYTLLTKKYQTWIKSNLVPPHTN

Target ORF information:

RefSeq Version	NM_002104.2
Organism	Homo sapiens(human)
Definition	Homo sapiens granzyme K (GZMK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002104.2

ORF Insert Sequence:

ATGACTAAGT TTTCTTCCTT TTCTCTGTTT TTCCTAATAG TTGGGGCTTA TATGACTCAT 
GTGTGTTTCA ATATGGAAAT TATTGGAGGG AAAGAAGTGT CACCTCATTC CAGGCCATTT 
ATGGCCTCCA TCCAGTATGG CGGACATCAC GTTTGTGGAG GTGTTCTGAT TGATCCACAG 
TGGGTGCTGA CAGCAGCCCA CTGCCAATAT CGGTTTACCA AAGGCCAGTC TCCCACTGTG 
GTTTTAGGCG CACACTCTCT CTCAAAGAAT GAGGCCTCCA AACAAACACT GGAGATCAAA 
AAATTTATAC CATTCTCAAG AGTTACATCA GATCCTCAAT CAAATGATAT CATGCTGGTT 
AAGCTTCAAA CAGCCGCAAA ACTCAATAAA CATGTCAAGA TGCTCCACAT AAGATCCAAA 
ACCTCTCTTA GATCTGGAAC CAAATGCAAG GTTACTGGCT GGGGAGCCAC CGATCCAGAT 
TCATTAAGAC CTTCTGACAC CCTGCGAGAA GTCACTGTTA CTGTCCTAAG TCGAAAACTT 
TGCAACAGCC AAAGTTACTA CAACGGCGAC CCTTTTATCA CCAAAGACAT GGTCTGTGCA 
GGAGATGCCA AAGGCCAGAA GGATTCCTGT AAGGGTGACT CAGGGGGCCC CTTGATCTGT 
AAAGGTGTCT TCCACGCTAT AGTCTCTGGA GGTCATGAAT GTGGTGTTGC CACAAAGCCT 
GGAATCTACA CCCTGTTAAC CAAGAAATAC CAGACTTGGA TCAAAAGCAA CCTTGTCCCG 
CCTCATACAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Extracellular granzyme K mediates endothelial activation through the cleavage of protease-activated receptor-1.
The FEBS journal283(9)1734-47(2016 05)
Sharma M,Merkulova Y,Raithatha S,Parkinson LG,Shen Y,Cooper D,Granville DJ

Effect of carbamate pesticides on perforin, granzymes A-B-3/K, and granulysin in human natural killer cells.
International journal of immunopathology and pharmacology28(3)403-10(2015 Sep)
Li Q,Kobayashi M,Kawada T

Substrate specificities of the granzyme tryptases A and K.
Journal of proteome research13(12)6067-77(2014 Dec)
Plasman K,Demol H,Bird PI,Gevaert K,Van Damme P

Granzyme K synergistically potentiates LPS-induced cytokine responses in human monocytes.
Proceedings of the National Academy of Sciences of the United States of America111(16)5974-9(2014 Apr)
Wensink AC,Kemp V,Fermie J,Garc?a Laorden MI,van der Poll T,Hack CE,Bovenschen N

Unexpected role for granzyme K in CD56bright NK cell-mediated immunoregulation of multiple sclerosis.
Journal of immunology (Baltimore, Md. : 1950)187(2)781-90(2011 Jul)
Jiang W,Chai NR,Maric D,Bielekova B

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GZMK cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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