Gnmt cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gnmt
Entrez Gene ID	14711
Full Name	glycine N-methyltransferase
General protein information	Preferred Names glycine N-methyltransferase Names glycine N-methyltransferase glycine N methyl transferase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 22.9 cM\|17 C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001364890.1	NP_001351819.1	glycine N-methyltransferase isoform 2
NM_010321.1	NP_034451.1	glycine N-methyltransferase isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GNMT	XP_518481.1
Bos taurus (cattle)	GNMT	NP_001193045.1
Danio rerio (zebrafish)	gnmt	NP_997981.1
Drosophila melanogaster (fruit fly)	CG6188	NP_650223.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002198	XP_565619.1
Canis lupus familiaris (dog)	GNMT	XP_532140.1
Homo sapiens (human)	GNMT	NP_061833.1
	GNMT	XP_001089876.1
Mus musculus (house mouse)	Gnmt	NP_034451.1

Gene Ontology
Bibliography

Related articles in PubMed

Glycine N-methyltransferase deficiency in female mice impairs insulin signaling and promotes gluconeogenesis by modulating the PI3K/Akt pathway in the liver.
Liao YJ, Lee TS, Twu YC, Hsu SM, Yang CP, Wang CK, Liang YC, Chen YA
Journal of biomedical science23(1)69(2016 Oct)

Niemann-Pick type C2 protein regulates liver cancer progression via modulating ERK1/2 pathway: Clinicopathological correlations and therapeutical implications.
Liao YJ, Fang CC, Yen CH, Hsu SM, Wang CK, Huang SF, Liang YC, Lin YY, Chu YT, Arthur Chen YM
International journal of cancer137(6)1341-51(2015 Sep)

Role of glycine N-methyltransferase in the regulation of T-cell responses in experimental autoimmune encephalomyelitis.
Li CH, Lin MH, Chu SH, Tu PH, Fang CC, Yen CH, Liang PI, Huang JC, Su YC, Sytwu HK, Chen YM
Molecular medicine (Cambridge, Mass.)20684-96(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hepatic GNMT regulates lipid and glucose homeostasis, and provide insight into the development of insulin resistance through modulating the PI3K/Akt pathway.
Title: Glycine N-methyltransferase deficiency in female mice impairs insulin signaling and promotes gluconeogenesis by modulating the PI3K/Akt pathway in the liver.

GNMT is involved in the pathogenesis of experimental autoimmune encephalomyelitis and plays a crucial role in the regulation of CD4+ T-cell functions
Title: Role of glycine N-methyltransferase in the regulation of T-cell responses in experimental autoimmune encephalomyelitis.

GNMT is found in the hippocampus and regulates intracellular SAMe levels. GNMT deficiency in mice results in high SAMe levels within the hippocampus, reduced neurogenic capacity, and spatial learning and memory impairment.
Title: Glycine N-methyltransferase expression in the hippocampus and its role in neurogenesis and cognitive performance.

It may be a crucial molecule in the pathogenesis of DSS-induced colitis.
Title: Role of glycine N-methyltransferase in experimental ulcerative colitis.

Reduced expression of GNMT in mouse hepatoma cells appears to increase the activity of LKB1/RAS signaling.
Title: Hepatoma cells from mice deficient in glycine N-methyltransferase have increased RAS signaling and activation of liver kinase B1.

The following Gnmt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gnmt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14574	NM_010321.1 Latest version!	Mus musculus glycine N-methyltransferase (Gnmt), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu84350	NM_001364890.1 Latest version!	Mus musculus glycine N-methyltransferase (Gnmt), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gnmt ( NM_010321.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034451.1 Mus musculus glycine N-methyltransferase (Gnmt), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14574
Clone ID Related Accession (Same CDS sequence)	NM_010321.1
Accession Version	NM_010321.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Mus musculus(house mouse)
Product	glycine N-methyltransferase isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from D89664.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D89664.1, BC087960.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849386, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGACA GCGTGTACCG TACCCGCTCC CTGGGGGTGG CGGCCGAAGG GCTCCCGGAC 
CAGTATGCAG ATGGGGAGGC CGCACGTGTG TGGCAGCTGT ACATCGGGGA CACCCGCAGC 
CGTACCGCAG AGTACAAGGC GTGGTTGCTT GGGCTGTTGC GCCAGCACGG GTGCCACAGG 
GTGCTGGACG TAGCCTGTGG CACAGGAGTG GACTCCATCA TGCTGGTGGA AGAGGGCTTC 
AGCGTGATGA GCGTGGACGC CAGCGACAAG ATGCTGAAAT ATGCGCTTAA GGAGCGCTGG 
AACCGGAGGA AAGAGCCATC CTTTGACAAT TGGGTCATTG AAGAAGCCAA CTGGTTGACG 
CTGGACAAAG ATGTGCTTTC AGGAGATGGC TTTGATGCTG TCATCTGCCT TGGGAACAGT 
TTTGCTCACT TGCCAGACTG CAAAGGTGAC CAGAGCGAGC ACCGGCTGGC ACTAAAGAAC 
ATTGCAAGCA TGGTGCGGCC CGGGGGCCTG CTGGTGATCG ACCACCGCAA CTACGACTAT 
ATCCTCAGCA CAGGCTGTGC GCCCCCGGGG AAGAACATCT ACTATAAGAG TGACCTGACC 
AAGGACATTA CGACGTCAGT ACTGACAGTC AACAACAAAG CCCACATGGT AACCCTGGAC 
TACACAGTGC AGGTGCCAGG CACTGGCAGA GATGGCTCTC CTGGCTTCAG TAAGTTCCGG 
CTCTCTTACT ACCCACACTG TTTGGCGTCT TTCACGGAGT TGGTGCGAGC AGCCTTTGGG 
GGCAGGTGCC AGCACAGCGT CCTGGGTGAC TTCAAGCCCT ACAAGCCTGG CCAGGCCTAC 
GTTCCCTGCT ACTTCATCCA TGTGCTCAAG AAGACAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034451.1
CDS	15..896
Translation	MVDSVYRTRSLGVAAEGLPDQYADGEAARVWQLYIGDTRSRTAEYKAWLLGLLRQHGCHRVLDVACGTGVDSIMLVEEGFSVMSVDASDKMLKYALKERWNRRKEPSFDNWVIEEANWLTLDKDVLSGDGFDAVICLGNSFAHLPDCKGDQSEHRLALKNIASMVRPGGLLVIDHRNYDYILSTGCAPPGKNIYYKSDLTKDITTSVLTVNNKAHMVTLDYTVQVPGTGRDGSPGFSKFRLSYYPHCLASFTELVRAAFGGRCQHSVLGDFKPYKPGQAYVPCYFIHVLKKTD

Target ORF information:

RefSeq Version	NM_010321.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus glycine N-methyltransferase (Gnmt), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010321.1

ORF Insert Sequence:

ATGGTGGACA GCGTGTACCG TACCCGCTCC CTGGGGGTGG CGGCCGAAGG GCTCCCGGAC 
CAGTATGCAG ATGGGGAGGC CGCACGTGTG TGGCAGCTGT ACATCGGGGA CACCCGCAGC 
CGTACCGCAG AGTACAAGGC GTGGTTGCTT GGGCTGTTGC GCCAGCACGG GTGCCACAGG 
GTGCTGGACG TAGCCTGTGG CACAGGAGTG GACTCCATCA TGCTGGTGGA AGAGGGCTTC 
AGCGTGATGA GCGTGGACGC CAGCGACAAG ATGCTGAAAT ATGCGCTTAA GGAGCGCTGG 
AACCGGAGGA AAGAGCCATC CTTTGACAAT TGGGTCATTG AAGAAGCCAA CTGGTTGACG 
CTGGACAAAG ATGTGCTTTC AGGAGATGGC TTTGATGCTG TCATCTGCCT TGGGAACAGT 
TTTGCTCACT TGCCAGACTG CAAAGGTGAC CAGAGCGAGC ACCGGCTGGC ACTAAAGAAC 
ATTGCAAGCA TGGTGCGGCC CGGGGGCCTG CTGGTGATCG ACCACCGCAA CTACGACTAT 
ATCCTCAGCA CAGGCTGTGC GCCCCCGGGG AAGAACATCT ACTATAAGAG TGACCTGACC 
AAGGACATTA CGACGTCAGT ACTGACAGTC AACAACAAAG CCCACATGGT AACCCTGGAC 
TACACAGTGC AGGTGCCAGG CACTGGCAGA GATGGCTCTC CTGGCTTCAG TAAGTTCCGG 
CTCTCTTACT ACCCACACTG TTTGGCGTCT TTCACGGAGT TGGTGCGAGC AGCCTTTGGG 
GGCAGGTGCC AGCACAGCGT CCTGGGTGAC TTCAAGCCCT ACAAGCCTGG CCAGGCCTAC 
GTTCCCTGCT ACTTCATCCA TGTGCTCAAG AAGACAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnmt ( NM_001364890.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001351819.1 Mus musculus glycine N-methyltransferase (Gnmt), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu84350
Clone ID Related Accession (Same CDS sequence)	NM_001364890.1
Accession Version	NM_001364890.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Mus musculus(house mouse)
Product	glycine N-methyltransferase isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT030702.11. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI327563.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849386, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGACA GCGTGTACCG TACCCGCTCC CTGGGGGTGG CGGCCGAAGG GCTCCCGGAC 
CAGTATGCAG ATGGGGAGGC CGCACGTGTG TGGCAGCTGT ACATCGGGGA CACCCGCAGC 
CGTACCGCAG AGTACAAGGC GTGGTTGCTT GGGCTGTTGC GCCAGCACGG GTGCCACAGG 
GTGCTGGACG TAGCCTGTGG CACAGGAGTG GACTCCATCA TGCTGGTGGA AGAGGGCTTC 
AGCGTGATGA GCGTGGACGC CAGCGACAAG ATGCTGAAAT ATGCGCTTAA GGAGCGCTGG 
AACCGGAGGA AAGAGCCATC CTTTGACAAT TGGGTCATTG AAGAAGCCAA CTGGTTGACG 
CTGGACAAAG ATGTGCTTTC AGGAGATGGC TTTGATGCTG TCATCTGCCT TGGGAACAGT 
TTTGCTCACT TGCCAGACTG CAAAGGCTGT GCGCCCCCGG GGAAGAACAT CTACTATAAG 
AGTGACCTGA CCAAGGACAT TACGACGTCA GTACTGACAG TCAACAACAA AGCCCACATG 
GTAACCCTGG ACTACACAGT GCAGGTGCCA GGCACTGGCA GAGATGGCTC TCCTGGCTTC 
AGTAAGTTCC GGCTCTCTTA CTACCCACAC TGTTTGGCGT CTTTCACGGA GTTGGTGCGA 
GCAGCCTTTG GGGGCAGGTG CCAGCACAGC GTCCTGGGTG ACTTCAAGCC CTACAAGCCT 
GGCCAGGCCT ACGTTCCCTG CTACTTCATC CATGTGCTCA AGAAGACAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001351819.1
CDS	15..788
Translation	MVDSVYRTRSLGVAAEGLPDQYADGEAARVWQLYIGDTRSRTAEYKAWLLGLLRQHGCHRVLDVACGTGVDSIMLVEEGFSVMSVDASDKMLKYALKERWNRRKEPSFDNWVIEEANWLTLDKDVLSGDGFDAVICLGNSFAHLPDCKGCAPPGKNIYYKSDLTKDITTSVLTVNNKAHMVTLDYTVQVPGTGRDGSPGFSKFRLSYYPHCLASFTELVRAAFGGRCQHSVLGDFKPYKPGQAYVPCYFIHVLKKTD

Target ORF information:

RefSeq Version	NM_001364890.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus glycine N-methyltransferase (Gnmt), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364890.1

ORF Insert Sequence:

ATGGTGGACA GCGTGTACCG TACCCGCTCC CTGGGGGTGG CGGCCGAAGG GCTCCCGGAC 
CAGTATGCAG ATGGGGAGGC CGCACGTGTG TGGCAGCTGT ACATCGGGGA CACCCGCAGC 
CGTACCGCAG AGTACAAGGC GTGGTTGCTT GGGCTGTTGC GCCAGCACGG GTGCCACAGG 
GTGCTGGACG TAGCCTGTGG CACAGGAGTG GACTCCATCA TGCTGGTGGA AGAGGGCTTC 
AGCGTGATGA GCGTGGACGC CAGCGACAAG ATGCTGAAAT ATGCGCTTAA GGAGCGCTGG 
AACCGGAGGA AAGAGCCATC CTTTGACAAT TGGGTCATTG AAGAAGCCAA CTGGTTGACG 
CTGGACAAAG ATGTGCTTTC AGGAGATGGC TTTGATGCTG TCATCTGCCT TGGGAACAGT 
TTTGCTCACT TGCCAGACTG CAAAGGCTGT GCGCCCCCGG GGAAGAACAT CTACTATAAG 
AGTGACCTGA CCAAGGACAT TACGACGTCA GTACTGACAG TCAACAACAA AGCCCACATG 
GTAACCCTGG ACTACACAGT GCAGGTGCCA GGCACTGGCA GAGATGGCTC TCCTGGCTTC 
AGTAAGTTCC GGCTCTCTTA CTACCCACAC TGTTTGGCGT CTTTCACGGA GTTGGTGCGA 
GCAGCCTTTG GGGGCAGGTG CCAGCACAGC GTCCTGGGTG ACTTCAAGCC CTACAAGCCT 
GGCCAGGCCT ACGTTCCCTG CTACTTCATC CATGTGCTCA AGAAGACAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glycine N-methyltransferase deficiency in female mice impairs insulin signaling and promotes gluconeogenesis by modulating the PI3K/Akt pathway in the liver.
Journal of biomedical science23(1)69(2016 Oct)
Liao YJ,Lee TS,Twu YC,Hsu SM,Yang CP,Wang CK,Liang YC,Chen YA

Niemann-Pick type C2 protein regulates liver cancer progression via modulating ERK1/2 pathway: Clinicopathological correlations and therapeutical implications.
International journal of cancer137(6)1341-51(2015 Sep)
Liao YJ,Fang CC,Yen CH,Hsu SM,Wang CK,Huang SF,Liang YC,Lin YY,Chu YT,Arthur Chen YM

Role of glycine N-methyltransferase in the regulation of T-cell responses in experimental autoimmune encephalomyelitis.
Molecular medicine (Cambridge, Mass.)20684-96(2015 Mar)
Li CH,Lin MH,Chu SH,Tu PH,Fang CC,Yen CH,Liang PI,Huang JC,Su YC,Sytwu HK,Chen YM

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Gnmt cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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