Bloc1s1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bloc1s1
Entrez Gene ID	14533
Full Name	biogenesis of lysosomal organelles complex-1, subunit 1
Synonyms	AI839753,BLOS1,Gcn5l1
General protein information	Preferred Names biogenesis of lysosomal organelles complex-1, subunit 1 Names biogenesis of lysosome-related organelles complex 1 subunit 1 BLOC-1 subunit 1 GCN5 general control of amino acid synthesis-like 1 GCN5-like protein 1 biogenesis of lysosome-related organelles complex-1, subunit 1 general control of amino acid synthesis, yeast homolog-like 1 general control of amino acid synthesis-like 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 D3\|10 77.19 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006513229.2	XP_006513292.1	biogenesis of lysosome-related organelles complex 1 subunit 1 isoform X1
XM_006513229.3	XP_006513292.1	biogenesis of lysosome-related organelles complex 1 subunit 1 isoform X1
NM_015740.3	NP_056555.2	biogenesis of lysosome-related organelles complex 1 subunit 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Bloc1s1	NP_056555.2
Xenopus tropicalis (tropical clawed frog)	bloc1s1	NP_001072249.1
Danio rerio (zebrafish)	LOC556597	NP_001037815.1
Drosophila melanogaster (fruit fly)	blos1	NP_725401.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001887	XP_321175.4
	BLOC1S1	XP_001095809.2
Homo sapiens (human)	BLOC1S1	NP_001478.2
Pan troglodytes (chimpanzee)	BLOC1S1	XP_509120.1
Bos taurus (cattle)	BLOC1S1	NP_001069830.1
Rattus norvegicus (Norway rat)	Bloc1s1	NP_001099411.2
Caenorhabditis elegans (roundworm)	CELE_T20G5.10	NP_499262.1

Gene Ontology
Bibliography

Related articles in PubMed

GCN5L1 modulates cross-talk between mitochondria and cell signaling to regulate FoxO1 stability and gluconeogenesis.
Wang L, Scott I, Zhu L, Wu K, Han K, Chen Y, Gucek M, Sack MN
Nature communications8(1)523(2017 09)

Acetylation of mitochondrial proteins by GCN5L1 promotes enhanced fatty acid oxidation in the heart.
Thapa D, Zhang M, Manning JR, Guimar?es DA, Stoner MW, O'Doherty RM, Shiva S, Scott I
American journal of physiology. Heart and circulatory physiology313(2)H265-H274(2017 Aug)

The TDH-GCN5L1-Fbxo15-KBP axis limits mitochondrial biogenesis in mouse embryonic stem?cells.
Donato V, Bonora M, Simoneschi D, Sartini D, Kudo Y, Saraf A, Florens L, Washburn MP, Stadtfeld M, Pinton P, Pagano M
Nature cell biology19(4)341-351(2017 Apr)

BLOS2 negatively regulates Notch signaling during neural and hematopoietic stem and progenitor cell development.
Zhou W, He Q, Zhang C, He X, Cui Z, Liu F, Li W
eLife5(2016 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that the mitochondrial enriched GCN5-like 1 protein (GCN5L1) controls hepatic glucose production by regulating FoxO1 protein levels.
Title: GCN5L1 modulates cross-talk between mitochondria and cell signaling to regulate FoxO1 stability and gluconeogenesis.

Acetylation of cardiac mitochondrial fatty acid oxidation enzymes by GCN5L1 significantly upregulates their activity in diet-induced obese mice.
Title: Acetylation of mitochondrial proteins by GCN5L1 promotes enhanced fatty acid oxidation in the heart.

TDH, responsible for mitochondrial production of acetyl-CoA in mouse embryonic stem cells, and the acetyltransferase GCN5L1 cooperate to acetylate Lys501 in KBP, allowing its recognition by and degradation via Fbxo15, an F-box protein transcriptionally controlled by the pluripotency core factors and repressed following differentiation.
Title: The TDH-GCN5L1-Fbxo15-KBP axis limits mitochondrial biogenesis in mouse embryonic stem?cells.

BLOS1 interacts with sorting nexin 2 and the ESCRT-I component TSG101 to mediate the sorting of EGFR into endosomal compartments
Title: Biogenesis of lysosome-related organelles complex-1 subunit 1 (BLOS1) interacts with sorting nexin 2 and the endosomal sorting complex required for transport-I (ESCRT-I) component TSG101 to mediate the sorting of epidermal growth factor receptor into endosomal compartments.

Here we show that genetic deletion of GCN5L1 has a direct positive effect on the expression and activity of Transcriptional Factor EB (TFEB), which acts as a master regulator of autophagy.
Title: GCN5-like protein 1 (GCN5L1) controls mitochondrial content through coordinated regulation of mitochondrial biogenesis and mitophagy.

The following Bloc1s1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bloc1s1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu17703	NM_015740.3 Latest version!	Mus musculus biogenesis of lysosomal organelles complex-1, subunit 1 (Bloc1s1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu17703	XM_006513229.3 Latest version!	Mus musculus biogenesis of lysosomal organelles complex-1, subunit 1 (Bloc1s1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OMu17703	XM_006513229.2	Mus musculus biogenesis of lysosomal organelles complex-1, subunit 1 (Bloc1s1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bloc1s1 ( NM_015740.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_056555.2 Mus musculus biogenesis of lysosomal organelles complex-1, subunit 1 (Bloc1s1), mRNA.

Price & Availability↑

CloneID	OMu17703
Clone ID Related Accession (Same CDS sequence)	XM_006513229.2 , XM_006513229.3 , NM_015740.3
Accession Version	NM_015740.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	biogenesis of lysosome-related organelles complex 1 subunit 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK003035.1. On Apr 6, 2007 this sequence version replaced NM_015740.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK003035.1, CD769731.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGCTGTCCC GCCTGCTCAA AGAACACCAA GCCAAGCAGA ACGAACGCAA GGAGCTGCAG 
GAGAAGAGGA GGAGAGAAGC TATCGCTGCA GCGACCTGCC TGACCGAAGC GTTGGTGGAT 
CACCTCAACG TGGGTGTGGC CCAGGCCTAC ATGAACCAGA GAAAGCTGGA CCATGAGGTG 
AAGACTCTAC AGGTCCAGGC TGCCCAGTTT GCCAAGCAGA CAGGCCAGTG GATTGGAATG 
GTGGAGAACT TCAACCAGGC ACTTAAGGAA ATTGGGGATG TGGAGAACTG GGCTAGGAGC 
ATCGAGCTGG ACATGCGCAC CATTGCCACT GCGCTGGAAT ACGTCTACAA AGGGCAGCTG 
CAGTCTGCAC CATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056555.2
CDS	90..467
Translation	MLSRLLKEHQAKQNERKELQEKRRREAIAAATCLTEALVDHLNVGVAQAYMNQRKLDHEVKTLQVQAAQFAKQTGQWIGMVENFNQALKEIGDVENWARSIELDMRTIATALEYVYKGQLQSAPS

Target ORF information:

RefSeq Version	NM_015740.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus biogenesis of lysosomal organelles complex-1, subunit 1 (Bloc1s1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015740.3

ORF Insert Sequence:

ATGCTGTCCC GCCTGCTCAA AGAACACCAA GCCAAGCAGA ACGAACGCAA GGAGCTGCAG 
GAGAAGAGGA GGAGAGAAGC TATCGCTGCA GCGACCTGCC TGACCGAAGC GTTGGTGGAT 
CACCTCAACG TGGGTGTGGC CCAGGCCTAC ATGAACCAGA GAAAGCTGGA CCATGAGGTG 
AAGACTCTAC AGGTCCAGGC TGCCCAGTTT GCCAAGCAGA CAGGCCAGTG GATTGGAATG 
GTGGAGAACT TCAACCAGGC ACTTAAGGAA ATTGGGGATG TGGAGAACTG GGCTAGGAGC 
ATCGAGCTGG ACATGCGCAC CATTGCCACT GCGCTGGAAT ACGTCTACAA AGGGCAGCTG 
CAGTCTGCAC CATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bloc1s1 ( XM_006513229.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513292.1 Mus musculus biogenesis of lysosomal organelles complex-1, subunit 1 (Bloc1s1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu17703
Clone ID Related Accession (Same CDS sequence)	XM_006513229.2 , XM_006513229.3 , NM_015740.3
Accession Version	XM_006513229.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	biogenesis of lysosome-related organelles complex 1 subunit 1 isoform X1
Comment

ATGCTGTCCC GCCTGCTCAA AGAACACCAA GCCAAGCAGA ACGAACGCAA GGAGCTGCAG 
GAGAAGAGGA GGAGAGAAGC TATCGCTGCA GCGACCTGCC TGACCGAAGC GTTGGTGGAT 
CACCTCAACG TGGGTGTGGC CCAGGCCTAC ATGAACCAGA GAAAGCTGGA CCATGAGGTG 
AAGACTCTAC AGGTCCAGGC TGCCCAGTTT GCCAAGCAGA CAGGCCAGTG GATTGGAATG 
GTGGAGAACT TCAACCAGGC ACTTAAGGAA ATTGGGGATG TGGAGAACTG GGCTAGGAGC 
ATCGAGCTGG ACATGCGCAC CATTGCCACT GCGCTGGAAT ACGTCTACAA AGGGCAGCTG 
CAGTCTGCAC CATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513292.1
Translation	MLSRLLKEHQAKQNERKELQEKRRREAIAAATCLTEALVDHLNVGVAQAYMNQRKLDHEVKTLQVQAAQFAKQTGQWIGMVENFNQALKEIGDVENWARSIELDMRTIATALEYVYKGQLQSAPS

Target ORF information:

RefSeq Version	XM_006513229.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus biogenesis of lysosomal organelles complex-1, subunit 1 (Bloc1s1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513229.2

ORF Insert Sequence:

ATGCTGTCCC GCCTGCTCAA AGAACACCAA GCCAAGCAGA ACGAACGCAA GGAGCTGCAG 
GAGAAGAGGA GGAGAGAAGC TATCGCTGCA GCGACCTGCC TGACCGAAGC GTTGGTGGAT 
CACCTCAACG TGGGTGTGGC CCAGGCCTAC ATGAACCAGA GAAAGCTGGA CCATGAGGTG 
AAGACTCTAC AGGTCCAGGC TGCCCAGTTT GCCAAGCAGA CAGGCCAGTG GATTGGAATG 
GTGGAGAACT TCAACCAGGC ACTTAAGGAA ATTGGGGATG TGGAGAACTG GGCTAGGAGC 
ATCGAGCTGG ACATGCGCAC CATTGCCACT GCGCTGGAAT ACGTCTACAA AGGGCAGCTG 
CAGTCTGCAC CATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bloc1s1 ( XM_006513229.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513292.1 Mus musculus biogenesis of lysosomal organelles complex-1, subunit 1 (Bloc1s1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu17703
Clone ID Related Accession (Same CDS sequence)	XM_006513229.2 , XM_006513229.3 , NM_015740.3
Accession Version	XM_006513229.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	biogenesis of lysosome-related organelles complex 1 subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513229.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCCC GCCTGCTCAA AGAACACCAA GCCAAGCAGA ACGAACGCAA GGAGCTGCAG 
GAGAAGAGGA GGAGAGAAGC TATCGCTGCA GCGACCTGCC TGACCGAAGC GTTGGTGGAT 
CACCTCAACG TGGGTGTGGC CCAGGCCTAC ATGAACCAGA GAAAGCTGGA CCATGAGGTG 
AAGACTCTAC AGGTCCAGGC TGCCCAGTTT GCCAAGCAGA CAGGCCAGTG GATTGGAATG 
GTGGAGAACT TCAACCAGGC ACTTAAGGAA ATTGGGGATG TGGAGAACTG GGCTAGGAGC 
ATCGAGCTGG ACATGCGCAC CATTGCCACT GCGCTGGAAT ACGTCTACAA AGGGCAGCTG 
CAGTCTGCAC CATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513292.1
CDS	116..493
Translation	MLSRLLKEHQAKQNERKELQEKRRREAIAAATCLTEALVDHLNVGVAQAYMNQRKLDHEVKTLQVQAAQFAKQTGQWIGMVENFNQALKEIGDVENWARSIELDMRTIATALEYVYKGQLQSAPS

Target ORF information:

RefSeq Version	XM_006513229.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus biogenesis of lysosomal organelles complex-1, subunit 1 (Bloc1s1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513229.3

ORF Insert Sequence:

ATGCTGTCCC GCCTGCTCAA AGAACACCAA GCCAAGCAGA ACGAACGCAA GGAGCTGCAG 
GAGAAGAGGA GGAGAGAAGC TATCGCTGCA GCGACCTGCC TGACCGAAGC GTTGGTGGAT 
CACCTCAACG TGGGTGTGGC CCAGGCCTAC ATGAACCAGA GAAAGCTGGA CCATGAGGTG 
AAGACTCTAC AGGTCCAGGC TGCCCAGTTT GCCAAGCAGA CAGGCCAGTG GATTGGAATG 
GTGGAGAACT TCAACCAGGC ACTTAAGGAA ATTGGGGATG TGGAGAACTG GGCTAGGAGC 
ATCGAGCTGG ACATGCGCAC CATTGCCACT GCGCTGGAAT ACGTCTACAA AGGGCAGCTG 
CAGTCTGCAC CATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GCN5L1 modulates cross-talk between mitochondria and cell signaling to regulate FoxO1 stability and gluconeogenesis.
Nature communications8(1)523(2017 09)
Wang L,Scott I,Zhu L,Wu K,Han K,Chen Y,Gucek M,Sack MN

Acetylation of mitochondrial proteins by GCN5L1 promotes enhanced fatty acid oxidation in the heart.
American journal of physiology. Heart and circulatory physiology313(2)H265-H274(2017 Aug)
Thapa D,Zhang M,Manning JR,Guimar?es DA,Stoner MW,O'Doherty RM,Shiva S,Scott I

The TDH-GCN5L1-Fbxo15-KBP axis limits mitochondrial biogenesis in mouse embryonic stem?cells.
Nature cell biology19(4)341-351(2017 Apr)
Donato V,Bonora M,Simoneschi D,Sartini D,Kudo Y,Saraf A,Florens L,Washburn MP,Stadtfeld M,Pinton P,Pagano M

BLOS2 negatively regulates Notch signaling during neural and hematopoietic stem and progenitor cell development.
eLife5(2016 10)
Zhou W,He Q,Zhang C,He X,Cui Z,Liu F,Li W

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Bloc1s1 cDNA ORF clone, Mus musculus(house mouse)

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Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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