Sesn1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sesn1
Entrez Gene ID	140742
Full Name	sestrin 1
Synonyms	1110002G11Rik,AU044290,Pa26,Sest1
General protein information	Preferred Names sestrin 1 Names sestrin-1 p53 regulated Pa26 nuclear protein p53-regulated protein PA26
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 B2\|10 22.77 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001013370.2	NP_001013388.2	sestrin-1 isoform 2
NM_001162908.1	NP_001156380.1	sestrin-1 isoform 1

Pathways from BioSystems

Homologies

	SESN1	XP_001092810.1
Canis lupus familiaris (dog)	SESN1	XP_854341.1
Mus musculus (house mouse)	Sesn1	NP_001156380.1
Bos taurus (cattle)	SESN1	NP_001094644.1
Rattus norvegicus (Norway rat)	Sesn1	NP_001099866.1
Gallus gallus (chicken)	SESN1	XP_419796.2
Xenopus tropicalis (tropical clawed frog)	sesn1	NP_001123411.1
Homo sapiens (human)	SESN1	NP_055269.1
Pan troglodytes (chimpanzee)	SESN1	XP_001150390.1
Danio rerio (zebrafish)	sesn1	NP_001002660.1

Gene Ontology
Bibliography

Related articles in PubMed

Sestrin 1 ameliorates cardiac hypertrophy via autophagy activation.
Xue R, Zeng J, Chen Y, Chen C, Tan W, Zhao J, Dong B, Sun Y, Dong Y, Liu C
Journal of cellular and molecular medicine21(6)1193-1205(2017 Jun)

A sestrin-dependent Erk-Jnk-p38 MAPK activation complex inhibits immunity during aging.
Lanna A, Gomes DC, Muller-Durovic B, McDonnell T, Escors D, Gilroy DW, Lee JH, Karin M, Akbar AN
Nature immunology18(3)354-363(2017 Mar)

Role of FEN1 S187 phosphorylation in counteracting oxygen-induced stress and regulating postnatal heart development.
Zhou L, Dai H, Wu J, Zhou M, Yuan H, Du J, Yang L, Wu X, Xu H, Hua Y, Xu J, Zheng L, Shen B
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(1)132-147(2017 01)

Sestrins function as guanine nucleotide dissociation inhibitors for Rag GTPases to control mTORC1 signaling.
Peng M, Yin N, Li MO
Cell159(1)122-133(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study found that Sestrins (1,2 and 3) can inhibit mTORC1 signaling in the absence of AMPK or TSC2. Surprisingly, when coexpressed with a GTP-bound constitutively active form of RagB (RagBQ99L), Sestrins potentiate mTORC1 signaling in the absence of amino acids.
Title: Sestrins function as guanine nucleotide dissociation inhibitors for Rag GTPases to control mTORC1 signaling.

Sestrin1 and Sestrin2 therefore provide an important link between genotoxic stress, p53 and the mTOR signaling pathway.
Title: p53 target genes sestrin1 and sestrin2 connect genotoxic stress and mTOR signaling.

The enhanced tumor susceptibility of p53+/- mice has made them one of several transgenic mouse models that are being considered as substitutes for standard 2-year rodent carcinogenicity assays.
Title: Is p53 haploinsufficient for tumor suppression? Implications for the p53+/- mouse model in carcinogenicity testing.

The following Sesn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sesn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08532	NM_001162908.1 Latest version!	Mus musculus sestrin 1 (Sesn1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$321.30 $459.00
OMu11433	NM_001013370.2 Latest version!	Mus musculus sestrin 1 (Sesn1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sesn1 ( NM_001162908.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156380.1 Mus musculus sestrin 1 (Sesn1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu08532
Clone ID Related Accession (Same CDS sequence)	NM_001162908.1
Accession Version	NM_001162908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-26
Organism	Mus musculus(house mouse)
Product	sestrin-1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC100403.1, AK160083.1 and BB338086.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC100403.1, SRR1660815.195672.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGAAG GAGAGAATGA AGCGAGATGG GAGGGACTGT GCAGCAGAGA CACGACTACT 
AGAGAGACAG CACTGGAAAA CATTAGGCAG ATCGTTTTGA GAAAAACCGA ATACCTTCGT 
TCAGCGAAAG AGACACTTTC CCGACCTTCA GACGGACTTT CAAACGCGGT TTCTTTGGAT 
GGGATGAATA AACTTCTTGC TCATCTGCTT ATGCTCTCTA AGAGATGTCC CTTTAAAGAT 
GTGCGAGAGA GAAGCGGGCT CATTCTGAAG AGCGTCGAGG AACTTGGAAT CAGAATCCCT 
CGGCCACTAG GACACGGACC AAGCAGGTTC ATCCCAGAGA AGGAGATGCT GCAGGTGGGC 
AGTGAAGACG CACAGATGCA TGCGTTATTT GCAGACTCCT TTGCTGCTTT GGGTCGTCTG 
GATAACATCA CATTAGTGAT GGTTTTCCAT CCACAATATT TAGAAAGTTT CTTAAAGACT 
CAACACTACC TACTACAAAT GGATGGGCCA TTACCCCTCC ATTATCGGCA CTACATTGGA 
ATCATGGCGG CAGCGAGGCA TCAGTGTTCC TACCTGGTGA ATCTGCATGT CAGTGACTTT 
CTTCATGTTG GTGGGGACCC CAAGTGGCTC AATGGTTTAG AGAATGCTCC TCAAAAACTG 
CAGAACTTAG GAGAACTTAA CAAAGTGTTA GCCCATAGGC CTTGGCTGAT TACCAAAGAA 
CATATCGAGG GCCTTCTGAA AGCAGAGGAG CACAGCTGGT CTCTCGCCGA GCTGGTCCAT 
GCCGTGGTTC TGCTCACACA CTACCACTCT CTTGCCTCCT TCACATTTGG CTGTGGAATT 
AGTCCAGAGA TTCATTGCGA TGGTGGCCAC ACCTTCAGAC CGCCTTCTGT TAGTAACTAC 
TGCATCTGTG ACATTACAAA TGGCAATCAC AGTGTGGATG AGATGCAAGT CAACTCAGCA 
GGAAATGCTT CGGTAAGTGA TTCCTTCTTT GAGGTGGAAG CTCTCATGGA AAAGATGAGG 
CAGTTACAGG AATGCCGAGA GGAGGAGGAG GCTAGCCAGG AGGAGATGGC GTCGCGGTTT 
GAGATGGAAA AGAGAGAGAG CATGTTTGTC TTCTCTTCGG ACGATGATGA AGTTACACCA 
GCAAGAGATG TGTCTCGGCA CTTTGAGGAC ACTAGTTACG GCTACAAGGA TTTCTCCAGA 
CACGGGATGC ATGTCCCAAC GTTTCGTGTC CAGGACTACT GCTGGGAAGA CCATGGTTAC 
TCTCTGGTGA ATCGTCTCTA TCCAGATGTG GGACAGTTAA TTGATGAGAA ATTTCACATT 
GCTTACAATC TAACTTACAA TACTATGGCC ATGCACAAAG ATGTTGATAC CTCAATGCTC 
AGACGGGCTA TCTGGAACTA TATCCACTGC ATGTTTGGAA TACGATACGA CGACTATGAC 
TATGGGGAAA TTAACCAGCT GCTGGATCGT AGCTTTAAAG TTTACATCAA AACCGTCGTG 
TGCACTCCTG AAAAGGTTAC CAAAAGGATG TATGACAGCT TCTGGAGGCA GTTCAAGCAC 
TCTGAGAAGG TCCATGTTAA TCTGCTTCTT ATAGAAGCTA GGATGCAAGC AGAACTCCTG 
TATGCTCTGA GAGCCATTAC CCGGTATATG ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156380.1
CDS	449..2104
Translation	MAEGENEARWEGLCSRDTTTRETALENIRQIVLRKTEYLRSAKETLSRPSDGLSNAVSLDGMNKLLAHLLMLSKRCPFKDVRERSGLILKSVEELGIRIPRPLGHGPSRFIPEKEMLQVGSEDAQMHALFADSFAALGRLDNITLVMVFHPQYLESFLKTQHYLLQMDGPLPLHYRHYIGIMAAARHQCSYLVNLHVSDFLHVGGDPKWLNGLENAPQKLQNLGELNKVLAHRPWLITKEHIEGLLKAEEHSWSLAELVHAVVLLTHYHSLASFTFGCGISPEIHCDGGHTFRPPSVSNYCICDITNGNHSVDEMQVNSAGNASVSDSFFEVEALMEKMRQLQECREEEEASQEEMASRFEMEKRESMFVFSSDDDEVTPARDVSRHFEDTSYGYKDFSRHGMHVPTFRVQDYCWEDHGYSLVNRLYPDVGQLIDEKFHIAYNLTYNTMAMHKDVDTSMLRRAIWNYIHCMFGIRYDDYDYGEINQLLDRSFKVYIKTVVCTPEKVTKRMYDSFWRQFKHSEKVHVNLLLIEARMQAELLYALRAITRYMT

Target ORF information:

RefSeq Version	NM_001162908.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sestrin 1 (Sesn1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001162908.1

ORF Insert Sequence:

ATGGCTGAAG GAGAGAATGA AGCGAGATGG GAGGGACTGT GCAGCAGAGA CACGACTACT 
AGAGAGACAG CACTGGAAAA CATTAGGCAG ATCGTTTTGA GAAAAACCGA ATACCTTCGT 
TCAGCGAAAG AGACACTTTC CCGACCTTCA GACGGACTTT CAAACGCGGT TTCTTTGGAT 
GGGATGAATA AACTTCTTGC TCATCTGCTT ATGCTCTCTA AGAGATGTCC CTTTAAAGAT 
GTGCGAGAGA GAAGCGGGCT CATTCTGAAG AGCGTCGAGG AACTTGGAAT CAGAATCCCT 
CGGCCACTAG GACACGGACC AAGCAGGTTC ATCCCAGAGA AGGAGATGCT GCAGGTGGGC 
AGTGAAGACG CACAGATGCA TGCGTTATTT GCAGACTCCT TTGCTGCTTT GGGTCGTCTG 
GATAACATCA CATTAGTGAT GGTTTTCCAT CCACAATATT TAGAAAGTTT CTTAAAGACT 
CAACACTACC TACTACAAAT GGATGGGCCA TTACCCCTCC ATTATCGGCA CTACATTGGA 
ATCATGGCGG CAGCGAGGCA TCAGTGTTCC TACCTGGTGA ATCTGCATGT CAGTGACTTT 
CTTCATGTTG GTGGGGACCC CAAGTGGCTC AATGGTTTAG AGAATGCTCC TCAAAAACTG 
CAGAACTTAG GAGAACTTAA CAAAGTGTTA GCCCATAGGC CTTGGCTGAT TACCAAAGAA 
CATATCGAGG GCCTTCTGAA AGCAGAGGAG CACAGCTGGT CTCTCGCCGA GCTGGTCCAT 
GCCGTGGTTC TGCTCACACA CTACCACTCT CTTGCCTCCT TCACATTTGG CTGTGGAATT 
AGTCCAGAGA TTCATTGCGA TGGTGGCCAC ACCTTCAGAC CGCCTTCTGT TAGTAACTAC 
TGCATCTGTG ACATTACAAA TGGCAATCAC AGTGTGGATG AGATGCAAGT CAACTCAGCA 
GGAAATGCTT CGGTAAGTGA TTCCTTCTTT GAGGTGGAAG CTCTCATGGA AAAGATGAGG 
CAGTTACAGG AATGCCGAGA GGAGGAGGAG GCTAGCCAGG AGGAGATGGC GTCGCGGTTT 
GAGATGGAAA AGAGAGAGAG CATGTTTGTC TTCTCTTCGG ACGATGATGA AGTTACACCA 
GCAAGAGATG TGTCTCGGCA CTTTGAGGAC ACTAGTTACG GCTACAAGGA TTTCTCCAGA 
CACGGGATGC ATGTCCCAAC GTTTCGTGTC CAGGACTACT GCTGGGAAGA CCATGGTTAC 
TCTCTGGTGA ATCGTCTCTA TCCAGATGTG GGACAGTTAA TTGATGAGAA ATTTCACATT 
GCTTACAATC TAACTTACAA TACTATGGCC ATGCACAAAG ATGTTGATAC CTCAATGCTC 
AGACGGGCTA TCTGGAACTA TATCCACTGC ATGTTTGGAA TACGATACGA CGACTATGAC 
TATGGGGAAA TTAACCAGCT GCTGGATCGT AGCTTTAAAG TTTACATCAA AACCGTCGTG 
TGCACTCCTG AAAAGGTTAC CAAAAGGATG TATGACAGCT TCTGGAGGCA GTTCAAGCAC 
TCTGAGAAGG TCCATGTTAA TCTGCTTCTT ATAGAAGCTA GGATGCAAGC AGAACTCCTG 
TATGCTCTGA GAGCCATTAC CCGGTATATG ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sesn1 ( NM_001013370.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001013388.2 Mus musculus sestrin 1 (Sesn1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu11433
Clone ID Related Accession (Same CDS sequence)	NM_001013370.2
Accession Version	NM_001013370.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-26
Organism	Mus musculus(house mouse)
Product	sestrin-1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY126656.1, BC055753.1, AK160083.1 and BB338086.1. On Jul 9, 2009 this sequence version replaced NM_001013370.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.145556.1, BC055753.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGCCTGG CCGCCGCGTC GAACGAGGCG TACGCAGCCT CGCTGGCTGT CTCCGAGCTG 
CTGAGCTGCC ACCAGTGCGG CGGGGACCGC GGCCAGGACG AGGAACTTGG AATCAGAATC 
CCTCGGCCAC TAGGACACGG ACCAAGCAGG TTCATCCCAG AGAAGGAGAT GCTGCAGGTG 
GGCAGTGAAG ACGCACAGAT GCATGCGTTA TTTGCAGACT CCTTTGCTGC TTTGGGTCGT 
CTGGATAACA TCACATTAGT GATGGTTTTC CATCCACAAT ATTTAGAAAG TTTCTTAAAG 
ACTCAACACT ACCTACTACA AATGGATGGG CCATTACCCC TCCATTATCG GCACTACATT 
GGAATCATGG CGGCAGCGAG GCATCAGTGT TCCTACCTGG TGAATCTGCA TGTCAGTGAC 
TTTCTTCATG TTGGTGGGGA CCCCAAGTGG CTCAATGGTT TAGAGAATGC TCCTCAAAAA 
CTGCAGAACT TAGGAGAACT TAACAAAGTG TTAGCCCATA GGCCTTGGCT GATTACCAAA 
GAACATATCG AGGGCCTTCT GAAAGCAGAG GAGCACAGCT GGTCTCTCGC CGAGCTGGTC 
CATGCCGTGG TTCTGCTCAC ACACTACCAC TCTCTTGCCT CCTTCACATT TGGCTGTGGA 
ATTAGTCCAG AGATTCATTG CGATGGTGGC CACACCTTCA GACCGCCTTC TGTTAGTAAC 
TACTGCATCT GTGACATTAC AAATGGCAAT CACAGTGTGG ATGAGATGCA AGTCAACTCA 
GCAGGAAATG CTTCGGTAAG TGATTCCTTC TTTGAGGTGG AAGCTCTCAT GGAAAAGATG 
AGGCAGTTAC AGGAATGCCG AGAGGAGGAG GAGGCTAGCC AGGAGGAGAT GGCGTCGCGG 
TTTGAGATGG AAAAGAGAGA GAGCATGTTT GTCTTCTCTT CGGACGATGA TGAAGTTACA 
CCAGCAAGAG ATGTGTCTCG GCACTTTGAG GACACTAGTT ACGGCTACAA GGATTTCTCC 
AGACACGGGA TGCATGTCCC AACGTTTCGT GTCCAGGACT ACTGCTGGGA AGACCATGGT 
TACTCTCTGG TGAATCGTCT CTATCCAGAT GTGGGACAGT TAATTGATGA GAAATTTCAC 
ATTGCTTACA ATCTAACTTA CAATACTATG GCCATGCACA AAGATGTTGA TACCTCAATG 
CTCAGACGGG CTATCTGGAA CTATATCCAC TGCATGTTTG GAATACGATA CGACGACTAT 
GACTATGGGG AAATTAACCA GCTGCTGGAT CGTAGCTTTA AAGTTTACAT CAAAACCGTC 
GTGTGCACTC CTGAAAAGGT TACCAAAAGG ATGTATGACA GCTTCTGGAG GCAGTTCAAG 
CACTCTGAGA AGGTCCATGT TAATCTGCTT CTTATAGAAG CTAGGATGCA AGCAGAACTC 
CTGTATGCTC TGAGAGCCAT TACCCGGTAT ATGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013388.2
CDS	56..1534
Translation	MRLAAASNEAYAASLAVSELLSCHQCGGDRGQDEELGIRIPRPLGHGPSRFIPEKEMLQVGSEDAQMHALFADSFAALGRLDNITLVMVFHPQYLESFLKTQHYLLQMDGPLPLHYRHYIGIMAAARHQCSYLVNLHVSDFLHVGGDPKWLNGLENAPQKLQNLGELNKVLAHRPWLITKEHIEGLLKAEEHSWSLAELVHAVVLLTHYHSLASFTFGCGISPEIHCDGGHTFRPPSVSNYCICDITNGNHSVDEMQVNSAGNASVSDSFFEVEALMEKMRQLQECREEEEASQEEMASRFEMEKRESMFVFSSDDDEVTPARDVSRHFEDTSYGYKDFSRHGMHVPTFRVQDYCWEDHGYSLVNRLYPDVGQLIDEKFHIAYNLTYNTMAMHKDVDTSMLRRAIWNYIHCMFGIRYDDYDYGEINQLLDRSFKVYIKTVVCTPEKVTKRMYDSFWRQFKHSEKVHVNLLLIEARMQAELLYALRAITRYMT

Target ORF information:

RefSeq Version	NM_001013370.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sestrin 1 (Sesn1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013370.2

ORF Insert Sequence:

ATGCGCCTGG CCGCCGCGTC GAACGAGGCG TACGCAGCCT CGCTGGCTGT CTCCGAGCTG 
CTGAGCTGCC ACCAGTGCGG CGGGGACCGC GGCCAGGACG AGGAACTTGG AATCAGAATC 
CCTCGGCCAC TAGGACACGG ACCAAGCAGG TTCATCCCAG AGAAGGAGAT GCTGCAGGTG 
GGCAGTGAAG ACGCACAGAT GCATGCGTTA TTTGCAGACT CCTTTGCTGC TTTGGGTCGT 
CTGGATAACA TCACATTAGT GATGGTTTTC CATCCACAAT ATTTAGAAAG TTTCTTAAAG 
ACTCAACACT ACCTACTACA AATGGATGGG CCATTACCCC TCCATTATCG GCACTACATT 
GGAATCATGG CGGCAGCGAG GCATCAGTGT TCCTACCTGG TGAATCTGCA TGTCAGTGAC 
TTTCTTCATG TTGGTGGGGA CCCCAAGTGG CTCAATGGTT TAGAGAATGC TCCTCAAAAA 
CTGCAGAACT TAGGAGAACT TAACAAAGTG TTAGCCCATA GGCCTTGGCT GATTACCAAA 
GAACATATCG AGGGCCTTCT GAAAGCAGAG GAGCACAGCT GGTCTCTCGC CGAGCTGGTC 
CATGCCGTGG TTCTGCTCAC ACACTACCAC TCTCTTGCCT CCTTCACATT TGGCTGTGGA 
ATTAGTCCAG AGATTCATTG CGATGGTGGC CACACCTTCA GACCGCCTTC TGTTAGTAAC 
TACTGCATCT GTGACATTAC AAATGGCAAT CACAGTGTGG ATGAGATGCA AGTCAACTCA 
GCAGGAAATG CTTCGGTAAG TGATTCCTTC TTTGAGGTGG AAGCTCTCAT GGAAAAGATG 
AGGCAGTTAC AGGAATGCCG AGAGGAGGAG GAGGCTAGCC AGGAGGAGAT GGCGTCGCGG 
TTTGAGATGG AAAAGAGAGA GAGCATGTTT GTCTTCTCTT CGGACGATGA TGAAGTTACA 
CCAGCAAGAG ATGTGTCTCG GCACTTTGAG GACACTAGTT ACGGCTACAA GGATTTCTCC 
AGACACGGGA TGCATGTCCC AACGTTTCGT GTCCAGGACT ACTGCTGGGA AGACCATGGT 
TACTCTCTGG TGAATCGTCT CTATCCAGAT GTGGGACAGT TAATTGATGA GAAATTTCAC 
ATTGCTTACA ATCTAACTTA CAATACTATG GCCATGCACA AAGATGTTGA TACCTCAATG 
CTCAGACGGG CTATCTGGAA CTATATCCAC TGCATGTTTG GAATACGATA CGACGACTAT 
GACTATGGGG AAATTAACCA GCTGCTGGAT CGTAGCTTTA AAGTTTACAT CAAAACCGTC 
GTGTGCACTC CTGAAAAGGT TACCAAAAGG ATGTATGACA GCTTCTGGAG GCAGTTCAAG 
CACTCTGAGA AGGTCCATGT TAATCTGCTT CTTATAGAAG CTAGGATGCA AGCAGAACTC 
CTGTATGCTC TGAGAGCCAT TACCCGGTAT ATGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sestrin 1 ameliorates cardiac hypertrophy via autophagy activation.
Journal of cellular and molecular medicine21(6)1193-1205(2017 Jun)
Xue R,Zeng J,Chen Y,Chen C,Tan W,Zhao J,Dong B,Sun Y,Dong Y,Liu C

A sestrin-dependent Erk-Jnk-p38 MAPK activation complex inhibits immunity during aging.
Nature immunology18(3)354-363(2017 Mar)
Lanna A,Gomes DC,Muller-Durovic B,McDonnell T,Escors D,Gilroy DW,Lee JH,Karin M,Akbar AN

Role of FEN1 S187 phosphorylation in counteracting oxygen-induced stress and regulating postnatal heart development.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(1)132-147(2017 01)
Zhou L,Dai H,Wu J,Zhou M,Yuan H,Du J,Yang L,Wu X,Xu H,Hua Y,Xu J,Zheng L,Shen B

Sestrins function as guanine nucleotide dissociation inhibitors for Rag GTPases to control mTORC1 signaling.
Cell159(1)122-133(2014 Sep)
Peng M,Yin N,Li MO