SESN1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SESN1
Entrez Gene ID	436933
Full Name	sestrin 1
Synonyms	si:ch211-51a19.4,zgc:103669,zgc:91970
General protein information	Preferred Names sestrin 1 Names sestrin-1 WITHDRAWN: zgc:103669
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005160673.4	XP_005160730.1	sestrin-1 isoform X3
XM_005160673.3	XP_005160730.1	sestrin-1 isoform X3
XM_005160670.4	XP_005160727.1	sestrin-1 isoform X1
XM_005160670.3	XP_005160727.1	sestrin-1 isoform X1
XM_005160671.4	XP_005160728.1	sestrin-1 isoform X2
NM_001002660.1	NP_001002660.1	sestrin-1
XM_005160671.3	XP_005160728.1	sestrin-1 isoform X2

Pathways from BioSystems

Homologies

	SESN1	XP_001092810.1
Canis lupus familiaris (dog)	SESN1	XP_854341.1
Mus musculus (house mouse)	Sesn1	NP_001156380.1
Bos taurus (cattle)	SESN1	NP_001094644.1
Rattus norvegicus (Norway rat)	Sesn1	NP_001099866.1
Gallus gallus (chicken)	SESN1	XP_419796.2
Xenopus tropicalis (tropical clawed frog)	sesn1	NP_001123411.1
Homo sapiens (human)	SESN1	NP_055269.1
Pan troglodytes (chimpanzee)	SESN1	XP_001150390.1
Danio rerio (zebrafish)	sesn1	NP_001002660.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of target genes downstream of semaphorin6A/PlexinA2 signaling in zebrafish.
Emerson SE, St Clair RM, Waldron AL, Bruno SR, Duong A, Driscoll HE, Ballif BA, McFarlane S, Ebert AM
Developmental dynamics : an official publication of the American Association of Anatomists246(7)539-549(2017 Jul)

Phylogenetic, syntenic, and tissue expression analysis of slc22 genes in zebrafish (Danio rerio).
Mihaljevic I, Popovic M, Zaja R, Smital T
BMC genomics17(1)626(2016 08)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Early embryonic gene expression profiling of zebrafish prion protein (Prp2) morphants.
Nourizadeh-Lillabadi R, Seil? Torgersen J, Vestrheim O, K?nig M, Alestr?m P, Syed M
PloS one5(10)e13573(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

identify SESN1 as an indispensable gene for vertebrate left-right asymmetry and a new player in mediating Nodal signaling
Title: Sesn1 is a novel gene for left-right asymmetry and mediating nodal signaling.

The following SESN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SESN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa31171	NM_001002660.1 Latest version!	Danio rerio sestrin 1 (sesn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa24957	XM_005160670.4 Latest version!	Danio rerio sestrin 1 (sesn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa24958	XM_005160671.4 Latest version!	Danio rerio sestrin 1 (sesn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa24960	XM_005160673.4 Latest version!	Danio rerio sestrin 1 (sesn1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa24960	XM_005160673.3	Danio rerio sestrin 1 (sesn1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa24957	XM_005160670.3	Danio rerio sestrin 1 (sesn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa24958	XM_005160671.3	Danio rerio sestrin 1 (sesn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SESN1 ( NM_001002660.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001002660.1 Danio rerio sestrin 1 (sesn1), mRNA.

Price & Availability↑

CloneID	ODa31171
Clone ID Related Accession (Same CDS sequence)	NM_001002660.1
Accession Version	NM_001002660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-12
Organism	Danio rerio(zebrafish)
Product	sestrin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC076550.1. ##Evidence-Data-START## Transcript exon combination :: BC076550.1, DQ858215.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGGCACG CATTGGCATC TAATGAAACT ATGGAAAACT CCTCTCTCAC CATGACAGAC 
TTGTTTAAAA TTTGCACACA GTGCGAGCGA CTTAGTAAAA AGGATGTTGG AGTGCGAATC 
CCCAGACCAG TGGGAAATGG ACCAAGTAGA TTTATACCAG AGAAAGAGAT ACTTCAAGTC 
AGTAAATTGG ATGAAAGGAC ACAGTCGATT TTTGAGGATG CCTTTGCAGT GCTTGGTCGC 
CTCGACAACA TCTCCTTGGT CATGGGGTTT CATCCTCAAT ATCTGGAGAG CTTTCTTAGG 
ACACAACATT ACCTCCTTCA GATGGACGGT CCATTGTCTC TGCACTGCAG GCATTATATT 
GGAATAATGG CTGCTGCTAG GCACCAGTGC ACGTATCTGG TCAACCTGCA CGTGAATGAC 
TTCCTGCAGG TCGGAGGTGA TCCGAAGTGG TTGAATGGTC TGGAAGAAGC CCCACGGAAG 
CTGCAGGCTC TCGGGGAGCT CAATAAGATC CTGGCCCACC GCCCCTGGCT GCTCACCAAA 
GAACACATTG AGCGTCTGCT GAAGGCAGAG GAGCACAGCT GGTCCCTGGC TGAGCTGATC 
CATGCTGTGG TGCTACTCAC ACACTATCAC TCTCTGGCCT CCTTCACGTT TGGTTGCGGC 
ATCACCCCTG ACATCCACAC AGATGGAGGA CACACGTTCA GGCCGCCCTC ACTCAGCGGG 
TACTGCGTTT GTGACATTGC CAATGGCAAC GGTGCCCAGG AGGATATGTT TGGGAACCAG 
CAGGTGTCAG AATCATCTGG TGAGGTGGAA GTGCTCATGG AGAGGATGAA GCAGCTTCAG 
GAATGTCGTG ATGAAGAAGA AGCCAGCCAG GAGGAAATGG CCACTCGTTT TGAGAGGGAG 
AAGACGGAGT GTATGCTGGT GGTCACCTCT GAAGATGAGG AGTGTGTGCC ATCCAGAGAT 
GTTTCGCGAC ACTTTGAAGA CCCTAGTTAT GGCTATAAAG ACTTCTCCAG GAGAGGAGAA 
CATGTACCAA CTCTCAGAGT GCAGGACTAC AGTTGGGAAG ATCATGGATT TTCCCTCGTG 
AATCGGCTGT ATTCGGATTT CGGCCAACTT CTGGATGAAA AGTTTCAGAT CGCGTTCAAT 
CTGACATACA ACACAATGGC AACACATCAG GGTGTGGACA CTTCCATGCT CCGCAGAGCC 
ATCTGGAACT ACATCCACTG CATGTTCGGG ATTCGGTACG ACGATTATGA TTATGGAGAA 
ATTAACCAAC TGTTAGACCG CAGTTTTAAA GTCTACATCA AAACAATGGT GTGCAGTCCA 
GAGAAAATCA CCAAGAGGAT GTACGAGAGC TTCTGGAGAC AGTTCCGGCA CTCTGAGAAA 
GTCCACGTTA ATTTGCTTCT TATGGAAGCG CGCATGCAGG CAGAACTGCT CTACGCTCTG 
AGAGCTATCA CTCGCTACAT GACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002660.1
CDS	115..1581
Translation	MRHALASNETMENSSLTMTDLFKICTQCERLSKKDVGVRIPRPVGNGPSRFIPEKEILQVSKLDERTQSIFEDAFAVLGRLDNISLVMGFHPQYLESFLRTQHYLLQMDGPLSLHCRHYIGIMAAARHQCTYLVNLHVNDFLQVGGDPKWLNGLEEAPRKLQALGELNKILAHRPWLLTKEHIERLLKAEEHSWSLAELIHAVVLLTHYHSLASFTFGCGITPDIHTDGGHTFRPPSLSGYCVCDIANGNGAQEDMFGNQQVSESSGEVEVLMERMKQLQECRDEEEASQEEMATRFEREKTECMLVVTSEDEECVPSRDVSRHFEDPSYGYKDFSRRGEHVPTLRVQDYSWEDHGFSLVNRLYSDFGQLLDEKFQIAFNLTYNTMATHQGVDTSMLRRAIWNYIHCMFGIRYDDYDYGEINQLLDRSFKVYIKTMVCSPEKITKRMYESFWRQFRHSEKVHVNLLLMEARMQAELLYALRAITRYMT

Target ORF information:

RefSeq Version	NM_001002660.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sestrin 1 (sesn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002660.1

ORF Insert Sequence:

ATGAGGCACG CATTGGCATC TAATGAAACT ATGGAAAACT CCTCTCTCAC CATGACAGAC 
TTGTTTAAAA TTTGCACACA GTGCGAGCGA CTTAGTAAAA AGGATGTTGG AGTGCGAATC 
CCCAGACCAG TGGGAAATGG ACCAAGTAGA TTTATACCAG AGAAAGAGAT ACTTCAAGTC 
AGTAAATTGG ATGAAAGGAC ACAGTCGATT TTTGAGGATG CCTTTGCAGT GCTTGGTCGC 
CTCGACAACA TCTCCTTGGT CATGGGGTTT CATCCTCAAT ATCTGGAGAG CTTTCTTAGG 
ACACAACATT ACCTCCTTCA GATGGACGGT CCATTGTCTC TGCACTGCAG GCATTATATT 
GGAATAATGG CTGCTGCTAG GCACCAGTGC ACGTATCTGG TCAACCTGCA CGTGAATGAC 
TTCCTGCAGG TCGGAGGTGA TCCGAAGTGG TTGAATGGTC TGGAAGAAGC CCCACGGAAG 
CTGCAGGCTC TCGGGGAGCT CAATAAGATC CTGGCCCACC GCCCCTGGCT GCTCACCAAA 
GAACACATTG AGCGTCTGCT GAAGGCAGAG GAGCACAGCT GGTCCCTGGC TGAGCTGATC 
CATGCTGTGG TGCTACTCAC ACACTATCAC TCTCTGGCCT CCTTCACGTT TGGTTGCGGC 
ATCACCCCTG ACATCCACAC AGATGGAGGA CACACGTTCA GGCCGCCCTC ACTCAGCGGG 
TACTGCGTTT GTGACATTGC CAATGGCAAC GGTGCCCAGG AGGATATGTT TGGGAACCAG 
CAGGTGTCAG AATCATCTGG TGAGGTGGAA GTGCTCATGG AGAGGATGAA GCAGCTTCAG 
GAATGTCGTG ATGAAGAAGA AGCCAGCCAG GAGGAAATGG CCACTCGTTT TGAGAGGGAG 
AAGACGGAGT GTATGCTGGT GGTCACCTCT GAAGATGAGG AGTGTGTGCC ATCCAGAGAT 
GTTTCGCGAC ACTTTGAAGA CCCTAGTTAT GGCTATAAAG ACTTCTCCAG GAGAGGAGAA 
CATGTACCAA CTCTCAGAGT GCAGGACTAC AGTTGGGAAG ATCATGGATT TTCCCTCGTG 
AATCGGCTGT ATTCGGATTT CGGCCAACTT CTGGATGAAA AGTTTCAGAT CGCGTTCAAT 
CTGACATACA ACACAATGGC AACACATCAG GGTGTGGACA CTTCCATGCT CCGCAGAGCC 
ATCTGGAACT ACATCCACTG CATGTTCGGG ATTCGGTACG ACGATTATGA TTATGGAGAA 
ATTAACCAAC TGTTAGACCG CAGTTTTAAA GTCTACATCA AAACAATGGT GTGCAGTCCA 
GAGAAAATCA CCAAGAGGAT GTACGAGAGC TTCTGGAGAC AGTTCCGGCA CTCTGAGAAA 
GTCCACGTTA ATTTGCTTCT TATGGAAGCG CGCATGCAGG CAGAACTGCT CTACGCTCTG 
AGAGCTATCA CTCGCTACAT GACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SESN1 ( XM_005160670.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160727.1 Danio rerio sestrin 1 (sesn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa24957
Clone ID Related Accession (Same CDS sequence)	XM_005160670.4 , XM_005160670.3
Accession Version	XM_005160670.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sestrin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160670.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTGA GAGATGCTGC AGGAAGATGG GATGCTTTGA GCAGTAGAGA CGCGAGCTCC 
AGAAACGCAG CGATGGAGCT CATTCAACAG GAGGTGATCA AGAAGATAGA GTCCATCGGG 
CCAATGCCAG ACCCTCAGTG CTCTTCTCCT CCTCCAACGA TGGATGACAG CCTGTCTAGT 
GATCTCAACG CGCTCCTGGC CCATGTCCTG ATGCTCTCCA AAAGATGTCC TTATGAAGAT 
GTCCGGGAGA AATGCATATG CTTGCTTCAG AGGGTTCAGG ATGTTGGAGT GCGAATCCCC 
AGACCAGTGG GAAATGGACC AAGTAGATTT ATACCAGAGA AAGAGGTTCA CCGCGTCTCT 
ACTCCCCTGT TGTCCCAGAT ACTTCAAGTC AGTAAATTGG ATGAAAGGAC ACAGTCGATT 
TTTGAGGATG CCTTTGCAGT GCTTGGTCGC CTCGACAACA TCTCCTTGGT CATGGGGTTT 
CATCCTCAAT ATCTGGAGAG CTTTCTTAGG ACACAACATT ACCTCCTTCA GATGGACGGT 
CCATTGTCTC TGCACTGCAG GCATTATATT GGAATAATGG CTGCTGCTAG GCACCAGTGC 
ACGTATCTGG TCAACCTGCA CGTGAATGAC TTCCTGCAGG TCGGAGGTGA TCCGAAGTGG 
TTGAATGGTC TGGAAGAAGC CCCACGGAAG CTGCAGGCTC TCGGGGAGCT CAATAAGATC 
CTGGCCCACC GCCCCTGGCT GCTCACCAAA GAACACATTG AGCGTCTGCT GAAGGCAGAG 
GAGCACAGCT GGTCCCTGGC TGAGCTGATC CATGCTGTGG TGCTACTCAC ACACTATCAC 
TCTCTGGCCT CCTTCACGTT TGGTTGCGGC ATCACCCCTG ACATCCACAC AGATGGAGGA 
CACACGTTCA GGCCGCCCTC ACTCAGCGGG TACTGCGTTT GTGACATTGC CAATGGCAAC 
GGTGCCCAGG AGGATATGTT TGGGAACCAG CAGGTGTCAG AATCATCTGG TGAGGTGGAA 
GTGCTCATGG AGAGGATGAA GCAGCTTCAG GAATGTCGTG ATGAAGAAGA AGCCAGCCAG 
GAGGAAATGG CCACTCGTTT TGAGAGGGAG AAGACGGAGT GTATGCTGGT GGTCACCTCT 
GAAGATGAGG AGTGTGTGCC ATCCAGAGAT GTTTCGCGAC ACTTTGAAGA CCCTAGTTAT 
GGCTATAAAG ACTTCTCCAG GAGAGGAGAA CATGTACCAA CTCTCAGAGT GCAGGACTAC 
AGTTGGGAAG ATCATGGATT TTCCCTCGTG AATCGGCTGT ATTCGGATTT CGGCCAACTT 
CTGGATGAAA AGTTTCAGAT CGCGTTCAAT CTGACATACA ACACAATGGC AACACATCAG 
GGTGTGGACA CTTCCATGCT CCGCAGAGCC ATCTGGAACT ACATCCACTG CATGTTCGGG 
ATTCGGTACG ACGATTATGA TTATGGAGAA ATTAACCAAC TGTTAGACCG CAGTTTTAAA 
GTCTACATCA AAACAATGGT GTGCAGTCCA GAGAAAATCA CCAAGAGGAT GTACGAGAGC 
TTCTGGAGAC AGTTCCGGCA CTCTGAGAAA GTCCACGTTA ATTTGCTTCT TATGGAAGCG 
CGCATGCAGG CAGAACTGCT CTACGCTCTG AGAGCTATCA CTCGCTACAT GACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160727.1
CDS	167..1843
Translation	MDVRDAAGRWDALSSRDASSRNAAMELIQQEVIKKIESIGPMPDPQCSSPPPTMDDSLSSDLNALLAHVLMLSKRCPYEDVREKCICLLQRVQDVGVRIPRPVGNGPSRFIPEKEVHRVSTPLLSQILQVSKLDERTQSIFEDAFAVLGRLDNISLVMGFHPQYLESFLRTQHYLLQMDGPLSLHCRHYIGIMAAARHQCTYLVNLHVNDFLQVGGDPKWLNGLEEAPRKLQALGELNKILAHRPWLLTKEHIERLLKAEEHSWSLAELIHAVVLLTHYHSLASFTFGCGITPDIHTDGGHTFRPPSLSGYCVCDIANGNGAQEDMFGNQQVSESSGEVEVLMERMKQLQECRDEEEASQEEMATRFEREKTECMLVVTSEDEECVPSRDVSRHFEDPSYGYKDFSRRGEHVPTLRVQDYSWEDHGFSLVNRLYSDFGQLLDEKFQIAFNLTYNTMATHQGVDTSMLRRAIWNYIHCMFGIRYDDYDYGEINQLLDRSFKVYIKTMVCSPEKITKRMYESFWRQFRHSEKVHVNLLLMEARMQAELLYALRAITRYMT

Target ORF information:

RefSeq Version	XM_005160670.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sestrin 1 (sesn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160670.4

ORF Insert Sequence:

ATGGACGTGA GAGATGCTGC AGGAAGATGG GATGCTTTGA GCAGTAGAGA CGCGAGCTCC 
AGAAACGCAG CGATGGAGCT CATTCAACAG GAGGTGATCA AGAAGATAGA GTCCATCGGG 
CCAATGCCAG ACCCTCAGTG CTCTTCTCCT CCTCCAACGA TGGATGACAG CCTGTCTAGT 
GATCTCAACG CGCTCCTGGC CCATGTCCTG ATGCTCTCCA AAAGATGTCC TTATGAAGAT 
GTCCGGGAGA AATGCATATG CTTGCTTCAG AGGGTTCAGG ATGTTGGAGT GCGAATCCCC 
AGACCAGTGG GAAATGGACC AAGTAGATTT ATACCAGAGA AAGAGGTTCA CCGCGTCTCT 
ACTCCCCTGT TGTCCCAGAT ACTTCAAGTC AGTAAATTGG ATGAAAGGAC ACAGTCGATT 
TTTGAGGATG CCTTTGCAGT GCTTGGTCGC CTCGACAACA TCTCCTTGGT CATGGGGTTT 
CATCCTCAAT ATCTGGAGAG CTTTCTTAGG ACACAACATT ACCTCCTTCA GATGGACGGT 
CCATTGTCTC TGCACTGCAG GCATTATATT GGAATAATGG CTGCTGCTAG GCACCAGTGC 
ACGTATCTGG TCAACCTGCA CGTGAATGAC TTCCTGCAGG TCGGAGGTGA TCCGAAGTGG 
TTGAATGGTC TGGAAGAAGC CCCACGGAAG CTGCAGGCTC TCGGGGAGCT CAATAAGATC 
CTGGCCCACC GCCCCTGGCT GCTCACCAAA GAACACATTG AGCGTCTGCT GAAGGCAGAG 
GAGCACAGCT GGTCCCTGGC TGAGCTGATC CATGCTGTGG TGCTACTCAC ACACTATCAC 
TCTCTGGCCT CCTTCACGTT TGGTTGCGGC ATCACCCCTG ACATCCACAC AGATGGAGGA 
CACACGTTCA GGCCGCCCTC ACTCAGCGGG TACTGCGTTT GTGACATTGC CAATGGCAAC 
GGTGCCCAGG AGGATATGTT TGGGAACCAG CAGGTGTCAG AATCATCTGG TGAGGTGGAA 
GTGCTCATGG AGAGGATGAA GCAGCTTCAG GAATGTCGTG ATGAAGAAGA AGCCAGCCAG 
GAGGAAATGG CCACTCGTTT TGAGAGGGAG AAGACGGAGT GTATGCTGGT GGTCACCTCT 
GAAGATGAGG AGTGTGTGCC ATCCAGAGAT GTTTCGCGAC ACTTTGAAGA CCCTAGTTAT 
GGCTATAAAG ACTTCTCCAG GAGAGGAGAA CATGTACCAA CTCTCAGAGT GCAGGACTAC 
AGTTGGGAAG ATCATGGATT TTCCCTCGTG AATCGGCTGT ATTCGGATTT CGGCCAACTT 
CTGGATGAAA AGTTTCAGAT CGCGTTCAAT CTGACATACA ACACAATGGC AACACATCAG 
GGTGTGGACA CTTCCATGCT CCGCAGAGCC ATCTGGAACT ACATCCACTG CATGTTCGGG 
ATTCGGTACG ACGATTATGA TTATGGAGAA ATTAACCAAC TGTTAGACCG CAGTTTTAAA 
GTCTACATCA AAACAATGGT GTGCAGTCCA GAGAAAATCA CCAAGAGGAT GTACGAGAGC 
TTCTGGAGAC AGTTCCGGCA CTCTGAGAAA GTCCACGTTA ATTTGCTTCT TATGGAAGCG 
CGCATGCAGG CAGAACTGCT CTACGCTCTG AGAGCTATCA CTCGCTACAT GACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SESN1 ( XM_005160670.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160727.1 Danio rerio sestrin 1 (sesn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa24957
Clone ID Related Accession (Same CDS sequence)	XM_005160670.4 , XM_005160670.3
Accession Version	XM_005160670.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sestrin-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160670.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTGA GAGATGCTGC AGGAAGATGG GATGCTTTGA GCAGTAGAGA CGCGAGCTCC 
AGAAACGCAG CGATGGAGCT CATTCAACAG GAGGTGATCA AGAAGATAGA GTCCATCGGG 
CCAATGCCAG ACCCTCAGTG CTCTTCTCCT CCTCCAACGA TGGATGACAG CCTGTCTAGT 
GATCTCAACG CGCTCCTGGC CCATGTCCTG ATGCTCTCCA AAAGATGTCC TTATGAAGAT 
GTCCGGGAGA AATGCATATG CTTGCTTCAG AGGGTTCAGG ATGTTGGAGT GCGAATCCCC 
AGACCAGTGG GAAATGGACC AAGTAGATTT ATACCAGAGA AAGAGGTTCA CCGCGTCTCT 
ACTCCCCTGT TGTCCCAGAT ACTTCAAGTC AGTAAATTGG ATGAAAGGAC ACAGTCGATT 
TTTGAGGATG CCTTTGCAGT GCTTGGTCGC CTCGACAACA TCTCCTTGGT CATGGGGTTT 
CATCCTCAAT ATCTGGAGAG CTTTCTTAGG ACACAACATT ACCTCCTTCA GATGGACGGT 
CCATTGTCTC TGCACTGCAG GCATTATATT GGAATAATGG CTGCTGCTAG GCACCAGTGC 
ACGTATCTGG TCAACCTGCA CGTGAATGAC TTCCTGCAGG TCGGAGGTGA TCCGAAGTGG 
TTGAATGGTC TGGAAGAAGC CCCACGGAAG CTGCAGGCTC TCGGGGAGCT CAATAAGATC 
CTGGCCCACC GCCCCTGGCT GCTCACCAAA GAACACATTG AGCGTCTGCT GAAGGCAGAG 
GAGCACAGCT GGTCCCTGGC TGAGCTGATC CATGCTGTGG TGCTACTCAC ACACTATCAC 
TCTCTGGCCT CCTTCACGTT TGGTTGCGGC ATCACCCCTG ACATCCACAC AGATGGAGGA 
CACACGTTCA GGCCGCCCTC ACTCAGCGGG TACTGCGTTT GTGACATTGC CAATGGCAAC 
GGTGCCCAGG AGGATATGTT TGGGAACCAG CAGGTGTCAG AATCATCTGG TGAGGTGGAA 
GTGCTCATGG AGAGGATGAA GCAGCTTCAG GAATGTCGTG ATGAAGAAGA AGCCAGCCAG 
GAGGAAATGG CCACTCGTTT TGAGAGGGAG AAGACGGAGT GTATGCTGGT GGTCACCTCT 
GAAGATGAGG AGTGTGTGCC ATCCAGAGAT GTTTCGCGAC ACTTTGAAGA CCCTAGTTAT 
GGCTATAAAG ACTTCTCCAG GAGAGGAGAA CATGTACCAA CTCTCAGAGT GCAGGACTAC 
AGTTGGGAAG ATCATGGATT TTCCCTCGTG AATCGGCTGT ATTCGGATTT CGGCCAACTT 
CTGGATGAAA AGTTTCAGAT CGCGTTCAAT CTGACATACA ACACAATGGC AACACATCAG 
GGTGTGGACA CTTCCATGCT CCGCAGAGCC ATCTGGAACT ACATCCACTG CATGTTCGGG 
ATTCGGTACG ACGATTATGA TTATGGAGAA ATTAACCAAC TGTTAGACCG CAGTTTTAAA 
GTCTACATCA AAACAATGGT GTGCAGTCCA GAGAAAATCA CCAAGAGGAT GTACGAGAGC 
TTCTGGAGAC AGTTCCGGCA CTCTGAGAAA GTCCACGTTA ATTTGCTTCT TATGGAAGCG 
CGCATGCAGG CAGAACTGCT CTACGCTCTG AGAGCTATCA CTCGCTACAT GACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160727.1
CDS	132..1808
Translation	MDVRDAAGRWDALSSRDASSRNAAMELIQQEVIKKIESIGPMPDPQCSSPPPTMDDSLSSDLNALLAHVLMLSKRCPYEDVREKCICLLQRVQDVGVRIPRPVGNGPSRFIPEKEVHRVSTPLLSQILQVSKLDERTQSIFEDAFAVLGRLDNISLVMGFHPQYLESFLRTQHYLLQMDGPLSLHCRHYIGIMAAARHQCTYLVNLHVNDFLQVGGDPKWLNGLEEAPRKLQALGELNKILAHRPWLLTKEHIERLLKAEEHSWSLAELIHAVVLLTHYHSLASFTFGCGITPDIHTDGGHTFRPPSLSGYCVCDIANGNGAQEDMFGNQQVSESSGEVEVLMERMKQLQECRDEEEASQEEMATRFEREKTECMLVVTSEDEECVPSRDVSRHFEDPSYGYKDFSRRGEHVPTLRVQDYSWEDHGFSLVNRLYSDFGQLLDEKFQIAFNLTYNTMATHQGVDTSMLRRAIWNYIHCMFGIRYDDYDYGEINQLLDRSFKVYIKTMVCSPEKITKRMYESFWRQFRHSEKVHVNLLLMEARMQAELLYALRAITRYMT

Target ORF information:

RefSeq Version	XM_005160670.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sestrin 1 (sesn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160670.3

ORF Insert Sequence:

ATGGACGTGA GAGATGCTGC AGGAAGATGG GATGCTTTGA GCAGTAGAGA CGCGAGCTCC 
AGAAACGCAG CGATGGAGCT CATTCAACAG GAGGTGATCA AGAAGATAGA GTCCATCGGG 
CCAATGCCAG ACCCTCAGTG CTCTTCTCCT CCTCCAACGA TGGATGACAG CCTGTCTAGT 
GATCTCAACG CGCTCCTGGC CCATGTCCTG ATGCTCTCCA AAAGATGTCC TTATGAAGAT 
GTCCGGGAGA AATGCATATG CTTGCTTCAG AGGGTTCAGG ATGTTGGAGT GCGAATCCCC 
AGACCAGTGG GAAATGGACC AAGTAGATTT ATACCAGAGA AAGAGGTTCA CCGCGTCTCT 
ACTCCCCTGT TGTCCCAGAT ACTTCAAGTC AGTAAATTGG ATGAAAGGAC ACAGTCGATT 
TTTGAGGATG CCTTTGCAGT GCTTGGTCGC CTCGACAACA TCTCCTTGGT CATGGGGTTT 
CATCCTCAAT ATCTGGAGAG CTTTCTTAGG ACACAACATT ACCTCCTTCA GATGGACGGT 
CCATTGTCTC TGCACTGCAG GCATTATATT GGAATAATGG CTGCTGCTAG GCACCAGTGC 
ACGTATCTGG TCAACCTGCA CGTGAATGAC TTCCTGCAGG TCGGAGGTGA TCCGAAGTGG 
TTGAATGGTC TGGAAGAAGC CCCACGGAAG CTGCAGGCTC TCGGGGAGCT CAATAAGATC 
CTGGCCCACC GCCCCTGGCT GCTCACCAAA GAACACATTG AGCGTCTGCT GAAGGCAGAG 
GAGCACAGCT GGTCCCTGGC TGAGCTGATC CATGCTGTGG TGCTACTCAC ACACTATCAC 
TCTCTGGCCT CCTTCACGTT TGGTTGCGGC ATCACCCCTG ACATCCACAC AGATGGAGGA 
CACACGTTCA GGCCGCCCTC ACTCAGCGGG TACTGCGTTT GTGACATTGC CAATGGCAAC 
GGTGCCCAGG AGGATATGTT TGGGAACCAG CAGGTGTCAG AATCATCTGG TGAGGTGGAA 
GTGCTCATGG AGAGGATGAA GCAGCTTCAG GAATGTCGTG ATGAAGAAGA AGCCAGCCAG 
GAGGAAATGG CCACTCGTTT TGAGAGGGAG AAGACGGAGT GTATGCTGGT GGTCACCTCT 
GAAGATGAGG AGTGTGTGCC ATCCAGAGAT GTTTCGCGAC ACTTTGAAGA CCCTAGTTAT 
GGCTATAAAG ACTTCTCCAG GAGAGGAGAA CATGTACCAA CTCTCAGAGT GCAGGACTAC 
AGTTGGGAAG ATCATGGATT TTCCCTCGTG AATCGGCTGT ATTCGGATTT CGGCCAACTT 
CTGGATGAAA AGTTTCAGAT CGCGTTCAAT CTGACATACA ACACAATGGC AACACATCAG 
GGTGTGGACA CTTCCATGCT CCGCAGAGCC ATCTGGAACT ACATCCACTG CATGTTCGGG 
ATTCGGTACG ACGATTATGA TTATGGAGAA ATTAACCAAC TGTTAGACCG CAGTTTTAAA 
GTCTACATCA AAACAATGGT GTGCAGTCCA GAGAAAATCA CCAAGAGGAT GTACGAGAGC 
TTCTGGAGAC AGTTCCGGCA CTCTGAGAAA GTCCACGTTA ATTTGCTTCT TATGGAAGCG 
CGCATGCAGG CAGAACTGCT CTACGCTCTG AGAGCTATCA CTCGCTACAT GACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SESN1 ( XM_005160671.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160728.1 Danio rerio sestrin 1 (sesn1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa24958
Clone ID Related Accession (Same CDS sequence)	XM_005160671.4 , XM_005160671.3
Accession Version	XM_005160671.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sestrin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160671.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTGA GAGATGCTGC AGGAAGATGG GATGCTTTGA GCAGTAGAGA CGCGAGCTCC 
AGAAACGCAG CGATGGAGCT CATTCAACAG GAGGTGATCA AGAAGATAGA GTCCATCGGG 
CCAATGCCAG ACCCTCAGTG CTCTTCTCCT CCTCCAACGA TGGATGACAG CCTGTCTAGT 
GATCTCAACG CGCTCCTGGC CCATGTCCTG ATGCTCTCCA AAAGATGTCC TTATGAAGAT 
GTCCGGGAGA AATGCATATG CTTGCTTCAG AGGGTTCAGG ATGTTGGAGT GCGAATCCCC 
AGACCAGTGG GAAATGGACC AAGTAGATTT ATACCAGAGA AAGAGATACT TCAAGTCAGT 
AAATTGGATG AAAGGACACA GTCGATTTTT GAGGATGCCT TTGCAGTGCT TGGTCGCCTC 
GACAACATCT CCTTGGTCAT GGGGTTTCAT CCTCAATATC TGGAGAGCTT TCTTAGGACA 
CAACATTACC TCCTTCAGAT GGACGGTCCA TTGTCTCTGC ACTGCAGGCA TTATATTGGA 
ATAATGGCTG CTGCTAGGCA CCAGTGCACG TATCTGGTCA ACCTGCACGT GAATGACTTC 
CTGCAGGTCG GAGGTGATCC GAAGTGGTTG AATGGTCTGG AAGAAGCCCC ACGGAAGCTG 
CAGGCTCTCG GGGAGCTCAA TAAGATCCTG GCCCACCGCC CCTGGCTGCT CACCAAAGAA 
CACATTGAGC GTCTGCTGAA GGCAGAGGAG CACAGCTGGT CCCTGGCTGA GCTGATCCAT 
GCTGTGGTGC TACTCACACA CTATCACTCT CTGGCCTCCT TCACGTTTGG TTGCGGCATC 
ACCCCTGACA TCCACACAGA TGGAGGACAC ACGTTCAGGC CGCCCTCACT CAGCGGGTAC 
TGCGTTTGTG ACATTGCCAA TGGCAACGGT GCCCAGGAGG ATATGTTTGG GAACCAGCAG 
GTGTCAGAAT CATCTGGTGA GGTGGAAGTG CTCATGGAGA GGATGAAGCA GCTTCAGGAA 
TGTCGTGATG AAGAAGAAGC CAGCCAGGAG GAAATGGCCA CTCGTTTTGA GAGGGAGAAG 
ACGGAGTGTA TGCTGGTGGT CACCTCTGAA GATGAGGAGT GTGTGCCATC CAGAGATGTT 
TCGCGACACT TTGAAGACCC TAGTTATGGC TATAAAGACT TCTCCAGGAG AGGAGAACAT 
GTACCAACTC TCAGAGTGCA GGACTACAGT TGGGAAGATC ATGGATTTTC CCTCGTGAAT 
CGGCTGTATT CGGATTTCGG CCAACTTCTG GATGAAAAGT TTCAGATCGC GTTCAATCTG 
ACATACAACA CAATGGCAAC ACATCAGGGT GTGGACACTT CCATGCTCCG CAGAGCCATC 
TGGAACTACA TCCACTGCAT GTTCGGGATT CGGTACGACG ATTATGATTA TGGAGAAATT 
AACCAACTGT TAGACCGCAG TTTTAAAGTC TACATCAAAA CAATGGTGTG CAGTCCAGAG 
AAAATCACCA AGAGGATGTA CGAGAGCTTC TGGAGACAGT TCCGGCACTC TGAGAAAGTC 
CACGTTAATT TGCTTCTTAT GGAAGCGCGC ATGCAGGCAG AACTGCTCTA CGCTCTGAGA 
GCTATCACTC GCTACATGAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160728.1
CDS	167..1810
Translation	MDVRDAAGRWDALSSRDASSRNAAMELIQQEVIKKIESIGPMPDPQCSSPPPTMDDSLSSDLNALLAHVLMLSKRCPYEDVREKCICLLQRVQDVGVRIPRPVGNGPSRFIPEKEILQVSKLDERTQSIFEDAFAVLGRLDNISLVMGFHPQYLESFLRTQHYLLQMDGPLSLHCRHYIGIMAAARHQCTYLVNLHVNDFLQVGGDPKWLNGLEEAPRKLQALGELNKILAHRPWLLTKEHIERLLKAEEHSWSLAELIHAVVLLTHYHSLASFTFGCGITPDIHTDGGHTFRPPSLSGYCVCDIANGNGAQEDMFGNQQVSESSGEVEVLMERMKQLQECRDEEEASQEEMATRFEREKTECMLVVTSEDEECVPSRDVSRHFEDPSYGYKDFSRRGEHVPTLRVQDYSWEDHGFSLVNRLYSDFGQLLDEKFQIAFNLTYNTMATHQGVDTSMLRRAIWNYIHCMFGIRYDDYDYGEINQLLDRSFKVYIKTMVCSPEKITKRMYESFWRQFRHSEKVHVNLLLMEARMQAELLYALRAITRYMT

Target ORF information:

RefSeq Version	XM_005160671.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sestrin 1 (sesn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160671.4

ORF Insert Sequence:

ATGGACGTGA GAGATGCTGC AGGAAGATGG GATGCTTTGA GCAGTAGAGA CGCGAGCTCC 
AGAAACGCAG CGATGGAGCT CATTCAACAG GAGGTGATCA AGAAGATAGA GTCCATCGGG 
CCAATGCCAG ACCCTCAGTG CTCTTCTCCT CCTCCAACGA TGGATGACAG CCTGTCTAGT 
GATCTCAACG CGCTCCTGGC CCATGTCCTG ATGCTCTCCA AAAGATGTCC TTATGAAGAT 
GTCCGGGAGA AATGCATATG CTTGCTTCAG AGGGTTCAGG ATGTTGGAGT GCGAATCCCC 
AGACCAGTGG GAAATGGACC AAGTAGATTT ATACCAGAGA AAGAGATACT TCAAGTCAGT 
AAATTGGATG AAAGGACACA GTCGATTTTT GAGGATGCCT TTGCAGTGCT TGGTCGCCTC 
GACAACATCT CCTTGGTCAT GGGGTTTCAT CCTCAATATC TGGAGAGCTT TCTTAGGACA 
CAACATTACC TCCTTCAGAT GGACGGTCCA TTGTCTCTGC ACTGCAGGCA TTATATTGGA 
ATAATGGCTG CTGCTAGGCA CCAGTGCACG TATCTGGTCA ACCTGCACGT GAATGACTTC 
CTGCAGGTCG GAGGTGATCC GAAGTGGTTG AATGGTCTGG AAGAAGCCCC ACGGAAGCTG 
CAGGCTCTCG GGGAGCTCAA TAAGATCCTG GCCCACCGCC CCTGGCTGCT CACCAAAGAA 
CACATTGAGC GTCTGCTGAA GGCAGAGGAG CACAGCTGGT CCCTGGCTGA GCTGATCCAT 
GCTGTGGTGC TACTCACACA CTATCACTCT CTGGCCTCCT TCACGTTTGG TTGCGGCATC 
ACCCCTGACA TCCACACAGA TGGAGGACAC ACGTTCAGGC CGCCCTCACT CAGCGGGTAC 
TGCGTTTGTG ACATTGCCAA TGGCAACGGT GCCCAGGAGG ATATGTTTGG GAACCAGCAG 
GTGTCAGAAT CATCTGGTGA GGTGGAAGTG CTCATGGAGA GGATGAAGCA GCTTCAGGAA 
TGTCGTGATG AAGAAGAAGC CAGCCAGGAG GAAATGGCCA CTCGTTTTGA GAGGGAGAAG 
ACGGAGTGTA TGCTGGTGGT CACCTCTGAA GATGAGGAGT GTGTGCCATC CAGAGATGTT 
TCGCGACACT TTGAAGACCC TAGTTATGGC TATAAAGACT TCTCCAGGAG AGGAGAACAT 
GTACCAACTC TCAGAGTGCA GGACTACAGT TGGGAAGATC ATGGATTTTC CCTCGTGAAT 
CGGCTGTATT CGGATTTCGG CCAACTTCTG GATGAAAAGT TTCAGATCGC GTTCAATCTG 
ACATACAACA CAATGGCAAC ACATCAGGGT GTGGACACTT CCATGCTCCG CAGAGCCATC 
TGGAACTACA TCCACTGCAT GTTCGGGATT CGGTACGACG ATTATGATTA TGGAGAAATT 
AACCAACTGT TAGACCGCAG TTTTAAAGTC TACATCAAAA CAATGGTGTG CAGTCCAGAG 
AAAATCACCA AGAGGATGTA CGAGAGCTTC TGGAGACAGT TCCGGCACTC TGAGAAAGTC 
CACGTTAATT TGCTTCTTAT GGAAGCGCGC ATGCAGGCAG AACTGCTCTA CGCTCTGAGA 
GCTATCACTC GCTACATGAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SESN1 ( XM_005160671.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160728.1 Danio rerio sestrin 1 (sesn1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa24958
Clone ID Related Accession (Same CDS sequence)	XM_005160671.4 , XM_005160671.3
Accession Version	XM_005160671.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sestrin-1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160671.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTGA GAGATGCTGC AGGAAGATGG GATGCTTTGA GCAGTAGAGA CGCGAGCTCC 
AGAAACGCAG CGATGGAGCT CATTCAACAG GAGGTGATCA AGAAGATAGA GTCCATCGGG 
CCAATGCCAG ACCCTCAGTG CTCTTCTCCT CCTCCAACGA TGGATGACAG CCTGTCTAGT 
GATCTCAACG CGCTCCTGGC CCATGTCCTG ATGCTCTCCA AAAGATGTCC TTATGAAGAT 
GTCCGGGAGA AATGCATATG CTTGCTTCAG AGGGTTCAGG ATGTTGGAGT GCGAATCCCC 
AGACCAGTGG GAAATGGACC AAGTAGATTT ATACCAGAGA AAGAGATACT TCAAGTCAGT 
AAATTGGATG AAAGGACACA GTCGATTTTT GAGGATGCCT TTGCAGTGCT TGGTCGCCTC 
GACAACATCT CCTTGGTCAT GGGGTTTCAT CCTCAATATC TGGAGAGCTT TCTTAGGACA 
CAACATTACC TCCTTCAGAT GGACGGTCCA TTGTCTCTGC ACTGCAGGCA TTATATTGGA 
ATAATGGCTG CTGCTAGGCA CCAGTGCACG TATCTGGTCA ACCTGCACGT GAATGACTTC 
CTGCAGGTCG GAGGTGATCC GAAGTGGTTG AATGGTCTGG AAGAAGCCCC ACGGAAGCTG 
CAGGCTCTCG GGGAGCTCAA TAAGATCCTG GCCCACCGCC CCTGGCTGCT CACCAAAGAA 
CACATTGAGC GTCTGCTGAA GGCAGAGGAG CACAGCTGGT CCCTGGCTGA GCTGATCCAT 
GCTGTGGTGC TACTCACACA CTATCACTCT CTGGCCTCCT TCACGTTTGG TTGCGGCATC 
ACCCCTGACA TCCACACAGA TGGAGGACAC ACGTTCAGGC CGCCCTCACT CAGCGGGTAC 
TGCGTTTGTG ACATTGCCAA TGGCAACGGT GCCCAGGAGG ATATGTTTGG GAACCAGCAG 
GTGTCAGAAT CATCTGGTGA GGTGGAAGTG CTCATGGAGA GGATGAAGCA GCTTCAGGAA 
TGTCGTGATG AAGAAGAAGC CAGCCAGGAG GAAATGGCCA CTCGTTTTGA GAGGGAGAAG 
ACGGAGTGTA TGCTGGTGGT CACCTCTGAA GATGAGGAGT GTGTGCCATC CAGAGATGTT 
TCGCGACACT TTGAAGACCC TAGTTATGGC TATAAAGACT TCTCCAGGAG AGGAGAACAT 
GTACCAACTC TCAGAGTGCA GGACTACAGT TGGGAAGATC ATGGATTTTC CCTCGTGAAT 
CGGCTGTATT CGGATTTCGG CCAACTTCTG GATGAAAAGT TTCAGATCGC GTTCAATCTG 
ACATACAACA CAATGGCAAC ACATCAGGGT GTGGACACTT CCATGCTCCG CAGAGCCATC 
TGGAACTACA TCCACTGCAT GTTCGGGATT CGGTACGACG ATTATGATTA TGGAGAAATT 
AACCAACTGT TAGACCGCAG TTTTAAAGTC TACATCAAAA CAATGGTGTG CAGTCCAGAG 
AAAATCACCA AGAGGATGTA CGAGAGCTTC TGGAGACAGT TCCGGCACTC TGAGAAAGTC 
CACGTTAATT TGCTTCTTAT GGAAGCGCGC ATGCAGGCAG AACTGCTCTA CGCTCTGAGA 
GCTATCACTC GCTACATGAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160728.1
CDS	132..1775
Translation	MDVRDAAGRWDALSSRDASSRNAAMELIQQEVIKKIESIGPMPDPQCSSPPPTMDDSLSSDLNALLAHVLMLSKRCPYEDVREKCICLLQRVQDVGVRIPRPVGNGPSRFIPEKEILQVSKLDERTQSIFEDAFAVLGRLDNISLVMGFHPQYLESFLRTQHYLLQMDGPLSLHCRHYIGIMAAARHQCTYLVNLHVNDFLQVGGDPKWLNGLEEAPRKLQALGELNKILAHRPWLLTKEHIERLLKAEEHSWSLAELIHAVVLLTHYHSLASFTFGCGITPDIHTDGGHTFRPPSLSGYCVCDIANGNGAQEDMFGNQQVSESSGEVEVLMERMKQLQECRDEEEASQEEMATRFEREKTECMLVVTSEDEECVPSRDVSRHFEDPSYGYKDFSRRGEHVPTLRVQDYSWEDHGFSLVNRLYSDFGQLLDEKFQIAFNLTYNTMATHQGVDTSMLRRAIWNYIHCMFGIRYDDYDYGEINQLLDRSFKVYIKTMVCSPEKITKRMYESFWRQFRHSEKVHVNLLLMEARMQAELLYALRAITRYMT

Target ORF information:

RefSeq Version	XM_005160671.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sestrin 1 (sesn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160671.3

ORF Insert Sequence:

ATGGACGTGA GAGATGCTGC AGGAAGATGG GATGCTTTGA GCAGTAGAGA CGCGAGCTCC 
AGAAACGCAG CGATGGAGCT CATTCAACAG GAGGTGATCA AGAAGATAGA GTCCATCGGG 
CCAATGCCAG ACCCTCAGTG CTCTTCTCCT CCTCCAACGA TGGATGACAG CCTGTCTAGT 
GATCTCAACG CGCTCCTGGC CCATGTCCTG ATGCTCTCCA AAAGATGTCC TTATGAAGAT 
GTCCGGGAGA AATGCATATG CTTGCTTCAG AGGGTTCAGG ATGTTGGAGT GCGAATCCCC 
AGACCAGTGG GAAATGGACC AAGTAGATTT ATACCAGAGA AAGAGATACT TCAAGTCAGT 
AAATTGGATG AAAGGACACA GTCGATTTTT GAGGATGCCT TTGCAGTGCT TGGTCGCCTC 
GACAACATCT CCTTGGTCAT GGGGTTTCAT CCTCAATATC TGGAGAGCTT TCTTAGGACA 
CAACATTACC TCCTTCAGAT GGACGGTCCA TTGTCTCTGC ACTGCAGGCA TTATATTGGA 
ATAATGGCTG CTGCTAGGCA CCAGTGCACG TATCTGGTCA ACCTGCACGT GAATGACTTC 
CTGCAGGTCG GAGGTGATCC GAAGTGGTTG AATGGTCTGG AAGAAGCCCC ACGGAAGCTG 
CAGGCTCTCG GGGAGCTCAA TAAGATCCTG GCCCACCGCC CCTGGCTGCT CACCAAAGAA 
CACATTGAGC GTCTGCTGAA GGCAGAGGAG CACAGCTGGT CCCTGGCTGA GCTGATCCAT 
GCTGTGGTGC TACTCACACA CTATCACTCT CTGGCCTCCT TCACGTTTGG TTGCGGCATC 
ACCCCTGACA TCCACACAGA TGGAGGACAC ACGTTCAGGC CGCCCTCACT CAGCGGGTAC 
TGCGTTTGTG ACATTGCCAA TGGCAACGGT GCCCAGGAGG ATATGTTTGG GAACCAGCAG 
GTGTCAGAAT CATCTGGTGA GGTGGAAGTG CTCATGGAGA GGATGAAGCA GCTTCAGGAA 
TGTCGTGATG AAGAAGAAGC CAGCCAGGAG GAAATGGCCA CTCGTTTTGA GAGGGAGAAG 
ACGGAGTGTA TGCTGGTGGT CACCTCTGAA GATGAGGAGT GTGTGCCATC CAGAGATGTT 
TCGCGACACT TTGAAGACCC TAGTTATGGC TATAAAGACT TCTCCAGGAG AGGAGAACAT 
GTACCAACTC TCAGAGTGCA GGACTACAGT TGGGAAGATC ATGGATTTTC CCTCGTGAAT 
CGGCTGTATT CGGATTTCGG CCAACTTCTG GATGAAAAGT TTCAGATCGC GTTCAATCTG 
ACATACAACA CAATGGCAAC ACATCAGGGT GTGGACACTT CCATGCTCCG CAGAGCCATC 
TGGAACTACA TCCACTGCAT GTTCGGGATT CGGTACGACG ATTATGATTA TGGAGAAATT 
AACCAACTGT TAGACCGCAG TTTTAAAGTC TACATCAAAA CAATGGTGTG CAGTCCAGAG 
AAAATCACCA AGAGGATGTA CGAGAGCTTC TGGAGACAGT TCCGGCACTC TGAGAAAGTC 
CACGTTAATT TGCTTCTTAT GGAAGCGCGC ATGCAGGCAG AACTGCTCTA CGCTCTGAGA 
GCTATCACTC GCTACATGAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SESN1 ( XM_005160673.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160730.1 Danio rerio sestrin 1 (sesn1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa24960
Clone ID Related Accession (Same CDS sequence)	XM_005160673.4 , XM_005160673.3
Accession Version	XM_005160673.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sestrin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160673.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCACG CATTGGCATC TAATGAAACT ATGGAAAACT CCTCTCTCAC CATGACAGAC 
TTGTTTAAAA TTTGCACACA GTGCGAGCGA CTTAGTAAAA AGGATGTTGG AGTGCGAATC 
CCCAGACCAG TGGGAAATGG ACCAAGTAGA TTTATACCAG AGAAAGAGGT TCACCGCGTC 
TCTACTCCCC TGTTGTCCCA GATACTTCAA GTCAGTAAAT TGGATGAAAG GACACAGTCG 
ATTTTTGAGG ATGCCTTTGC AGTGCTTGGT CGCCTCGACA ACATCTCCTT GGTCATGGGG 
TTTCATCCTC AATATCTGGA GAGCTTTCTT AGGACACAAC ATTACCTCCT TCAGATGGAC 
GGTCCATTGT CTCTGCACTG CAGGCATTAT ATTGGAATAA TGGCTGCTGC TAGGCACCAG 
TGCACGTATC TGGTCAACCT GCACGTGAAT GACTTCCTGC AGGTCGGAGG TGATCCGAAG 
TGGTTGAATG GTCTGGAAGA AGCCCCACGG AAGCTGCAGG CTCTCGGGGA GCTCAATAAG 
ATCCTGGCCC ACCGCCCCTG GCTGCTCACC AAAGAACACA TTGAGCGTCT GCTGAAGGCA 
GAGGAGCACA GCTGGTCCCT GGCTGAGCTG ATCCATGCTG TGGTGCTACT CACACACTAT 
CACTCTCTGG CCTCCTTCAC GTTTGGTTGC GGCATCACCC CTGACATCCA CACAGATGGA 
GGACACACGT TCAGGCCGCC CTCACTCAGC GGGTACTGCG TTTGTGACAT TGCCAATGGC 
AACGGTGCCC AGGAGGATAT GTTTGGGAAC CAGCAGGTGT CAGAATCATC TGGTGAGGTG 
GAAGTGCTCA TGGAGAGGAT GAAGCAGCTT CAGGAATGTC GTGATGAAGA AGAAGCCAGC 
CAGGAGGAAA TGGCCACTCG TTTTGAGAGG GAGAAGACGG AGTGTATGCT GGTGGTCACC 
TCTGAAGATG AGGAGTGTGT GCCATCCAGA GATGTTTCGC GACACTTTGA AGACCCTAGT 
TATGGCTATA AAGACTTCTC CAGGAGAGGA GAACATGTAC CAACTCTCAG AGTGCAGGAC 
TACAGTTGGG AAGATCATGG ATTTTCCCTC GTGAATCGGC TGTATTCGGA TTTCGGCCAA 
CTTCTGGATG AAAAGTTTCA GATCGCGTTC AATCTGACAT ACAACACAAT GGCAACACAT 
CAGGGTGTGG ACACTTCCAT GCTCCGCAGA GCCATCTGGA ACTACATCCA CTGCATGTTC 
GGGATTCGGT ACGACGATTA TGATTATGGA GAAATTAACC AACTGTTAGA CCGCAGTTTT 
AAAGTCTACA TCAAAACAAT GGTGTGCAGT CCAGAGAAAA TCACCAAGAG GATGTACGAG 
AGCTTCTGGA GACAGTTCCG GCACTCTGAG AAAGTCCACG TTAATTTGCT TCTTATGGAA 
GCGCGCATGC AGGCAGAACT GCTCTACGCT CTGAGAGCTA TCACTCGCTA CATGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160730.1
CDS	146..1645
Translation	MRHALASNETMENSSLTMTDLFKICTQCERLSKKDVGVRIPRPVGNGPSRFIPEKEVHRVSTPLLSQILQVSKLDERTQSIFEDAFAVLGRLDNISLVMGFHPQYLESFLRTQHYLLQMDGPLSLHCRHYIGIMAAARHQCTYLVNLHVNDFLQVGGDPKWLNGLEEAPRKLQALGELNKILAHRPWLLTKEHIERLLKAEEHSWSLAELIHAVVLLTHYHSLASFTFGCGITPDIHTDGGHTFRPPSLSGYCVCDIANGNGAQEDMFGNQQVSESSGEVEVLMERMKQLQECRDEEEASQEEMATRFEREKTECMLVVTSEDEECVPSRDVSRHFEDPSYGYKDFSRRGEHVPTLRVQDYSWEDHGFSLVNRLYSDFGQLLDEKFQIAFNLTYNTMATHQGVDTSMLRRAIWNYIHCMFGIRYDDYDYGEINQLLDRSFKVYIKTMVCSPEKITKRMYESFWRQFRHSEKVHVNLLLMEARMQAELLYALRAITRYMT

Target ORF information:

RefSeq Version	XM_005160673.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sestrin 1 (sesn1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160673.4

ORF Insert Sequence:

ATGAGGCACG CATTGGCATC TAATGAAACT ATGGAAAACT CCTCTCTCAC CATGACAGAC 
TTGTTTAAAA TTTGCACACA GTGCGAGCGA CTTAGTAAAA AGGATGTTGG AGTGCGAATC 
CCCAGACCAG TGGGAAATGG ACCAAGTAGA TTTATACCAG AGAAAGAGGT TCACCGCGTC 
TCTACTCCCC TGTTGTCCCA GATACTTCAA GTCAGTAAAT TGGATGAAAG GACACAGTCG 
ATTTTTGAGG ATGCCTTTGC AGTGCTTGGT CGCCTCGACA ACATCTCCTT GGTCATGGGG 
TTTCATCCTC AATATCTGGA GAGCTTTCTT AGGACACAAC ATTACCTCCT TCAGATGGAC 
GGTCCATTGT CTCTGCACTG CAGGCATTAT ATTGGAATAA TGGCTGCTGC TAGGCACCAG 
TGCACGTATC TGGTCAACCT GCACGTGAAT GACTTCCTGC AGGTCGGAGG TGATCCGAAG 
TGGTTGAATG GTCTGGAAGA AGCCCCACGG AAGCTGCAGG CTCTCGGGGA GCTCAATAAG 
ATCCTGGCCC ACCGCCCCTG GCTGCTCACC AAAGAACACA TTGAGCGTCT GCTGAAGGCA 
GAGGAGCACA GCTGGTCCCT GGCTGAGCTG ATCCATGCTG TGGTGCTACT CACACACTAT 
CACTCTCTGG CCTCCTTCAC GTTTGGTTGC GGCATCACCC CTGACATCCA CACAGATGGA 
GGACACACGT TCAGGCCGCC CTCACTCAGC GGGTACTGCG TTTGTGACAT TGCCAATGGC 
AACGGTGCCC AGGAGGATAT GTTTGGGAAC CAGCAGGTGT CAGAATCATC TGGTGAGGTG 
GAAGTGCTCA TGGAGAGGAT GAAGCAGCTT CAGGAATGTC GTGATGAAGA AGAAGCCAGC 
CAGGAGGAAA TGGCCACTCG TTTTGAGAGG GAGAAGACGG AGTGTATGCT GGTGGTCACC 
TCTGAAGATG AGGAGTGTGT GCCATCCAGA GATGTTTCGC GACACTTTGA AGACCCTAGT 
TATGGCTATA AAGACTTCTC CAGGAGAGGA GAACATGTAC CAACTCTCAG AGTGCAGGAC 
TACAGTTGGG AAGATCATGG ATTTTCCCTC GTGAATCGGC TGTATTCGGA TTTCGGCCAA 
CTTCTGGATG AAAAGTTTCA GATCGCGTTC AATCTGACAT ACAACACAAT GGCAACACAT 
CAGGGTGTGG ACACTTCCAT GCTCCGCAGA GCCATCTGGA ACTACATCCA CTGCATGTTC 
GGGATTCGGT ACGACGATTA TGATTATGGA GAAATTAACC AACTGTTAGA CCGCAGTTTT 
AAAGTCTACA TCAAAACAAT GGTGTGCAGT CCAGAGAAAA TCACCAAGAG GATGTACGAG 
AGCTTCTGGA GACAGTTCCG GCACTCTGAG AAAGTCCACG TTAATTTGCT TCTTATGGAA 
GCGCGCATGC AGGCAGAACT GCTCTACGCT CTGAGAGCTA TCACTCGCTA CATGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SESN1 ( XM_005160673.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160730.1 Danio rerio sestrin 1 (sesn1), transcript variant X3, mRNA.

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CloneID	ODa24960
Clone ID Related Accession (Same CDS sequence)	XM_005160673.4 , XM_005160673.3
Accession Version	XM_005160673.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sestrin-1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160673.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCACG CATTGGCATC TAATGAAACT ATGGAAAACT CCTCTCTCAC CATGACAGAC 
TTGTTTAAAA TTTGCACACA GTGCGAGCGA CTTAGTAAAA AGGATGTTGG AGTGCGAATC 
CCCAGACCAG TGGGAAATGG ACCAAGTAGA TTTATACCAG AGAAAGAGGT TCACCGCGTC 
TCTACTCCCC TGTTGTCCCA GATACTTCAA GTCAGTAAAT TGGATGAAAG GACACAGTCG 
ATTTTTGAGG ATGCCTTTGC AGTGCTTGGT CGCCTCGACA ACATCTCCTT GGTCATGGGG 
TTTCATCCTC AATATCTGGA GAGCTTTCTT AGGACACAAC ATTACCTCCT TCAGATGGAC 
GGTCCATTGT CTCTGCACTG CAGGCATTAT ATTGGAATAA TGGCTGCTGC TAGGCACCAG 
TGCACGTATC TGGTCAACCT GCACGTGAAT GACTTCCTGC AGGTCGGAGG TGATCCGAAG 
TGGTTGAATG GTCTGGAAGA AGCCCCACGG AAGCTGCAGG CTCTCGGGGA GCTCAATAAG 
ATCCTGGCCC ACCGCCCCTG GCTGCTCACC AAAGAACACA TTGAGCGTCT GCTGAAGGCA 
GAGGAGCACA GCTGGTCCCT GGCTGAGCTG ATCCATGCTG TGGTGCTACT CACACACTAT 
CACTCTCTGG CCTCCTTCAC GTTTGGTTGC GGCATCACCC CTGACATCCA CACAGATGGA 
GGACACACGT TCAGGCCGCC CTCACTCAGC GGGTACTGCG TTTGTGACAT TGCCAATGGC 
AACGGTGCCC AGGAGGATAT GTTTGGGAAC CAGCAGGTGT CAGAATCATC TGGTGAGGTG 
GAAGTGCTCA TGGAGAGGAT GAAGCAGCTT CAGGAATGTC GTGATGAAGA AGAAGCCAGC 
CAGGAGGAAA TGGCCACTCG TTTTGAGAGG GAGAAGACGG AGTGTATGCT GGTGGTCACC 
TCTGAAGATG AGGAGTGTGT GCCATCCAGA GATGTTTCGC GACACTTTGA AGACCCTAGT 
TATGGCTATA AAGACTTCTC CAGGAGAGGA GAACATGTAC CAACTCTCAG AGTGCAGGAC 
TACAGTTGGG AAGATCATGG ATTTTCCCTC GTGAATCGGC TGTATTCGGA TTTCGGCCAA 
CTTCTGGATG AAAAGTTTCA GATCGCGTTC AATCTGACAT ACAACACAAT GGCAACACAT 
CAGGGTGTGG ACACTTCCAT GCTCCGCAGA GCCATCTGGA ACTACATCCA CTGCATGTTC 
GGGATTCGGT ACGACGATTA TGATTATGGA GAAATTAACC AACTGTTAGA CCGCAGTTTT 
AAAGTCTACA TCAAAACAAT GGTGTGCAGT CCAGAGAAAA TCACCAAGAG GATGTACGAG 
AGCTTCTGGA GACAGTTCCG GCACTCTGAG AAAGTCCACG TTAATTTGCT TCTTATGGAA 
GCGCGCATGC AGGCAGAACT GCTCTACGCT CTGAGAGCTA TCACTCGCTA CATGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160730.1
CDS	150..1649
Translation	MRHALASNETMENSSLTMTDLFKICTQCERLSKKDVGVRIPRPVGNGPSRFIPEKEVHRVSTPLLSQILQVSKLDERTQSIFEDAFAVLGRLDNISLVMGFHPQYLESFLRTQHYLLQMDGPLSLHCRHYIGIMAAARHQCTYLVNLHVNDFLQVGGDPKWLNGLEEAPRKLQALGELNKILAHRPWLLTKEHIERLLKAEEHSWSLAELIHAVVLLTHYHSLASFTFGCGITPDIHTDGGHTFRPPSLSGYCVCDIANGNGAQEDMFGNQQVSESSGEVEVLMERMKQLQECRDEEEASQEEMATRFEREKTECMLVVTSEDEECVPSRDVSRHFEDPSYGYKDFSRRGEHVPTLRVQDYSWEDHGFSLVNRLYSDFGQLLDEKFQIAFNLTYNTMATHQGVDTSMLRRAIWNYIHCMFGIRYDDYDYGEINQLLDRSFKVYIKTMVCSPEKITKRMYESFWRQFRHSEKVHVNLLLMEARMQAELLYALRAITRYMT

Target ORF information:

RefSeq Version	XM_005160673.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sestrin 1 (sesn1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160673.3

ORF Insert Sequence:

ATGAGGCACG CATTGGCATC TAATGAAACT ATGGAAAACT CCTCTCTCAC CATGACAGAC 
TTGTTTAAAA TTTGCACACA GTGCGAGCGA CTTAGTAAAA AGGATGTTGG AGTGCGAATC 
CCCAGACCAG TGGGAAATGG ACCAAGTAGA TTTATACCAG AGAAAGAGGT TCACCGCGTC 
TCTACTCCCC TGTTGTCCCA GATACTTCAA GTCAGTAAAT TGGATGAAAG GACACAGTCG 
ATTTTTGAGG ATGCCTTTGC AGTGCTTGGT CGCCTCGACA ACATCTCCTT GGTCATGGGG 
TTTCATCCTC AATATCTGGA GAGCTTTCTT AGGACACAAC ATTACCTCCT TCAGATGGAC 
GGTCCATTGT CTCTGCACTG CAGGCATTAT ATTGGAATAA TGGCTGCTGC TAGGCACCAG 
TGCACGTATC TGGTCAACCT GCACGTGAAT GACTTCCTGC AGGTCGGAGG TGATCCGAAG 
TGGTTGAATG GTCTGGAAGA AGCCCCACGG AAGCTGCAGG CTCTCGGGGA GCTCAATAAG 
ATCCTGGCCC ACCGCCCCTG GCTGCTCACC AAAGAACACA TTGAGCGTCT GCTGAAGGCA 
GAGGAGCACA GCTGGTCCCT GGCTGAGCTG ATCCATGCTG TGGTGCTACT CACACACTAT 
CACTCTCTGG CCTCCTTCAC GTTTGGTTGC GGCATCACCC CTGACATCCA CACAGATGGA 
GGACACACGT TCAGGCCGCC CTCACTCAGC GGGTACTGCG TTTGTGACAT TGCCAATGGC 
AACGGTGCCC AGGAGGATAT GTTTGGGAAC CAGCAGGTGT CAGAATCATC TGGTGAGGTG 
GAAGTGCTCA TGGAGAGGAT GAAGCAGCTT CAGGAATGTC GTGATGAAGA AGAAGCCAGC 
CAGGAGGAAA TGGCCACTCG TTTTGAGAGG GAGAAGACGG AGTGTATGCT GGTGGTCACC 
TCTGAAGATG AGGAGTGTGT GCCATCCAGA GATGTTTCGC GACACTTTGA AGACCCTAGT 
TATGGCTATA AAGACTTCTC CAGGAGAGGA GAACATGTAC CAACTCTCAG AGTGCAGGAC 
TACAGTTGGG AAGATCATGG ATTTTCCCTC GTGAATCGGC TGTATTCGGA TTTCGGCCAA 
CTTCTGGATG AAAAGTTTCA GATCGCGTTC AATCTGACAT ACAACACAAT GGCAACACAT 
CAGGGTGTGG ACACTTCCAT GCTCCGCAGA GCCATCTGGA ACTACATCCA CTGCATGTTC 
GGGATTCGGT ACGACGATTA TGATTATGGA GAAATTAACC AACTGTTAGA CCGCAGTTTT 
AAAGTCTACA TCAAAACAAT GGTGTGCAGT CCAGAGAAAA TCACCAAGAG GATGTACGAG 
AGCTTCTGGA GACAGTTCCG GCACTCTGAG AAAGTCCACG TTAATTTGCT TCTTATGGAA 
GCGCGCATGC AGGCAGAACT GCTCTACGCT CTGAGAGCTA TCACTCGCTA CATGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of target genes downstream of semaphorin6A/PlexinA2 signaling in zebrafish.
Developmental dynamics : an official publication of the American Association of Anatomists246(7)539-549(2017 Jul)
Emerson SE,St Clair RM,Waldron AL,Bruno SR,Duong A,Driscoll HE,Ballif BA,McFarlane S,Ebert AM

Phylogenetic, syntenic, and tissue expression analysis of slc22 genes in zebrafish (Danio rerio).
BMC genomics17(1)626(2016 08)
Mihaljevic I,Popovic M,Zaja R,Smital T

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Early embryonic gene expression profiling of zebrafish prion protein (Prp2) morphants.
PloS one5(10)e13573(2010 Oct)
Nourizadeh-Lillabadi R,Seil? Torgersen J,Vestrheim O,K?nig M,Alestr?m P,Syed M

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SESN1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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