Cdk2ap1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cdk2ap1
Entrez Gene ID	13445
Full Name	CDK2 (cyclin-dependent kinase 2)-associated protein 1
Synonyms	Apc10,Cdkap1,DORC1,Doc1,ST19,doc-1,p12
General protein information	Preferred Names CDK2 (cyclin-dependent kinase 2)-associated protein 1 Names cyclin-dependent kinase 2-associated protein 1 CDK2-associated protein 1 deleted in oral cancer 1 putative oral cancer suppressor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 F

mRNA and Protein(s)

mRNA	Protein	Name
NM_013812.2	NP_038840.2	cyclin-dependent kinase 2-associated protein 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CDK2AP1	NP_004633.1
Danio rerio (zebrafish)	LOC100535265	XP_003199445.1
Bos taurus (cattle)	CDK2AP1	NP_001069837.1
Rattus norvegicus (Norway rat)	Cdk2ap1	NP_001107223.1
Pan troglodytes (chimpanzee)	CDK2AP1	XP_003952393.1
	CDK2AP1	XP_001100820.1
Mus musculus (house mouse)	Cdk2ap1	NP_038840.2
Xenopus tropicalis (tropical clawed frog)	cdk2ap1	NP_001016730.1
Canis lupus familiaris (dog)	CDK2AP1	NP_001234905.1
Gallus gallus (chicken)	CDK2AP1	NP_001034353.2

Gene Ontology
Bibliography

Related articles in PubMed

Conserved molecular signatures of neurogenesis in the hippocampal subgranular zone of rodents and primates.
Miller JA, Nathanson J, Franjic D, Shim S, Dalley RA, Shapouri S, Smith KA, Sunkin SM, Bernard A, Bennett JL, Lee CK, Hawrylycz MJ, Jones AR, Amaral DG, ?estan N, Gage FH, Lein ES
Development (Cambridge, England)140(22)4633-44(2013 Nov)

Progesterone is primary regulator of Cdk2ap1 gene expression and tissue-specific expression in the uterus.
Cheon YP, Kim CH
Journal of endocrinological investigation33(9)650-6(2010 Oct)

CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex.
Spruijt CG, Bartels SJ, Brinkman AB, Tjeertes JV, Poser I, Stunnenberg HG, Vermeulen M
Molecular bioSystems6(9)1700-6(2010 Sep)

The trafficking protein Tmed2/p24beta(1) is required for morphogenesis of the mouse embryo and placenta.
Jerome-Majewska LA, Achkar T, Luo L, Lupu F, Lacy E
Developmental biology341(1)154-66(2010 May)

Cyclin-dependent kinase 2-associating protein 1 commits murine embryonic stem cell differentiation through retinoblastoma protein regulation.
Kim Y, Deshpande A, Dai Y, Kim JJ, Lindgren A, Conway A, Clark AT, Wong DT
The Journal of biological chemistry284(35)23405-14(2009 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cdk2ap1 expression is primarily regulated by progesterone and the progesterone receptor in uterus and is mainly localized to proliferating tissues.
Title: Progesterone is primary regulator of Cdk2ap1 gene expression and tissue-specific expression in the uterus.

Results indicate that DOC-1 is a bona fide subunit of the Mi-2/NuRD chromatin remodeling complex.
Title: CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex.

CDK2AP1-mediated differentiation of murine embryonic stem cell was elicited through the regulation of pRb.
Title: Cyclin-dependent kinase 2-associating protein 1 commits murine embryonic stem cell differentiation through retinoblastoma protein regulation.

Cdk2ap1, a negative regulator of Cdk2 function and cell cycle, promotes Oct4 promoter methylation during murine embryonic stem cell differentiation to down-regulate Oct4 expression.
Title: Cdk2ap1 is required for epigenetic silencing of Oct4 during murine embryonic stem cell differentiation.

Cdk2ap1 plays an essential role in the early stage of embryogenesis and has a potential role during craniofacial morphogenesis.
Title: Targeted inactivation of p12, CDK2 associating protein 1, leads to early embryonic lethality.

The following Cdk2ap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cdk2ap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10002	NM_013812.2 Latest version!	Mus musculus CDK2 (cyclin-dependent kinase 2)-associated protein 1 (Cdk2ap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cdk2ap1 ( NM_013812.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038840.2 Mus musculus CDK2 (cyclin-dependent kinase 2)-associated protein 1 (Cdk2ap1), mRNA.

Price & Availability↑

CloneID	OMu10002
Clone ID Related Accession (Same CDS sequence)	NM_013812.2
Accession Version	NM_013812.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	cyclin-dependent kinase 2-associated protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE623061.1, AK004852.1 and AA190126.1. On or before May 4, 2006 this sequence version replaced XM_991893.1, NM_013812.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC083075.1, AK004852.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTCGTACA AGCCGAACTT GACCGCGCAC ATGCCCGCCG CCGCCCTCAA CGCCGGAAGT 
GTCCACTCAC CCTCTACTAG CATGGCGACC TCCTCCCAAT ATCGCCAGCT GCTGAGTGAC 
TATGGGCCTC CATCGCTAGG CTACACCCAG GGAACTGGAA ATAGCCAGGT GCCTCAGAGT 
AAATATGCAG AGCTGCTGGC CATCATTGAA GAGTTGGGGA AAGAGATCAG ACCGACGTAT 
GCAGGGAGCA AGAGCGCCAT GGAGAGGCTA AAACGAGGCA TCATTCATGC CCGGAGCCTG 
GTTCGGGAGT GCTTGGCTGA AACGGAACGG AATGCCAGGT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038840.2
CDS	258..602
Translation	MSYKPNLTAHMPAAALNAGSVHSPSTSMATSSQYRQLLSDYGPPSLGYTQGTGNSQVPQSKYAELLAIIEELGKEIRPTYAGSKSAMERLKRGIIHARSLVRECLAETERNARS

Target ORF information:

RefSeq Version	NM_013812.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus CDK2 (cyclin-dependent kinase 2)-associated protein 1 (Cdk2ap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013812.2

ORF Insert Sequence:

ATGTCGTACA AGCCGAACTT GACCGCGCAC ATGCCCGCCG CCGCCCTCAA CGCCGGAAGT 
GTCCACTCAC CCTCTACTAG CATGGCGACC TCCTCCCAAT ATCGCCAGCT GCTGAGTGAC 
TATGGGCCTC CATCGCTAGG CTACACCCAG GGAACTGGAA ATAGCCAGGT GCCTCAGAGT 
AAATATGCAG AGCTGCTGGC CATCATTGAA GAGTTGGGGA AAGAGATCAG ACCGACGTAT 
GCAGGGAGCA AGAGCGCCAT GGAGAGGCTA AAACGAGGCA TCATTCATGC CCGGAGCCTG 
GTTCGGGAGT GCTTGGCTGA AACGGAACGG AATGCCAGGT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Conserved molecular signatures of neurogenesis in the hippocampal subgranular zone of rodents and primates.
Development (Cambridge, England)140(22)4633-44(2013 Nov)
Miller JA,Nathanson J,Franjic D,Shim S,Dalley RA,Shapouri S,Smith KA,Sunkin SM,Bernard A,Bennett JL,Lee CK,Hawrylycz MJ,Jones AR,Amaral DG,?estan N,Gage FH,Lein ES

Progesterone is primary regulator of Cdk2ap1 gene expression and tissue-specific expression in the uterus.
Journal of endocrinological investigation33(9)650-6(2010 Oct)
Cheon YP,Kim CH

CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex.
Molecular bioSystems6(9)1700-6(2010 Sep)
Spruijt CG,Bartels SJ,Brinkman AB,Tjeertes JV,Poser I,Stunnenberg HG,Vermeulen M

The trafficking protein Tmed2/p24beta(1) is required for morphogenesis of the mouse embryo and placenta.
Developmental biology341(1)154-66(2010 May)
Jerome-Majewska LA,Achkar T,Luo L,Lupu F,Lacy E

Cyclin-dependent kinase 2-associating protein 1 commits murine embryonic stem cell differentiation through retinoblastoma protein regulation.
The Journal of biological chemistry284(35)23405-14(2009 Aug)
Kim Y,Deshpande A,Dai Y,Kim JJ,Lindgren A,Conway A,Clark AT,Wong DT