Crybb2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Crybb2
Entrez Gene ID	12961
Full Name	crystallin, beta B2
Synonyms	Cryb-2
General protein information	Preferred Names crystallin, beta B2 Names beta-crystallin B2 beta-crystallin Bp
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 F\|5 55.38 cM
Summary	This gene is a member of the beta-crystallin family. Beta crystallins, along with alpha and gamma crystallins, are the major proteins found in the eye lens. These proteins maintain the refractive index of the lens whilst also maintaining its transparency. Since lens central fiber cells lose their nuclei during development, crystallins are made and then retained throughout life, making them extremely stable proteins. Beta and gamma crystallins are considered be a superfamily and have a similar domain architecture, including four Greek Key motifs. Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. The protein encoded by this gene may have Ca2+-binding activity and could be associated with potential functions in the hippocampus and in sperm. Targeted knockout of this gene in mouse induces age-related cataract. A chain-terminating mutation in a similar gene in human was found to cause type 2 cerulean cataracts. [provided by RefSeq, Feb 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006534760.1	XP_006534823.1	beta-crystallin B2 isoform X1
NM_007773.4	NP_031799.1	beta-crystallin B2
XM_006534760.2	XP_006534823.2	beta-crystallin B2 isoform X1
XM_017320652.1	XP_017176141.1	beta-crystallin B2 isoform X2
XM_006534759.1	XP_006534822.1	beta-crystallin B2 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CRYBB2	NP_777232.1
Danio rerio (zebrafish)	crybb2	NP_001018138.1
Homo sapiens (human)	CRYBB2	NP_000487.1
Canis lupus familiaris (dog)	CRYBB2	NP_001041578.1
Mus musculus (house mouse)	Crybb2	NP_031799.1
Rattus norvegicus (Norway rat)	Crybb2	NP_037069.1
Gallus gallus (chicken)	CRYBB2	NP_990506.2
Pan troglodytes (chimpanzee)	CRYBB2	XP_003317196.1
	CRYBB2	NP_001116366.1
Xenopus tropicalis (tropical clawed frog)	crybb2	NP_001238879.1

Gene Ontology
Bibliography

Related articles in PubMed

N-cadherin regulates signaling mechanisms required for lens fiber cell elongation and lens morphogenesis.
Logan CM, Rajakaruna S, Bowen C, Radice GL, Robinson ML, Menko AS
Developmental biology428(1)118-134(2017 08)

Long non-coding RNAs regulate effects of ?-crystallin B2 on mouse ovary development.
Gao Q, Ren H, Chen M, Niu Z, Tao H, Jia Y, Zhang J, Li W
Molecular medicine reports14(5)4223-4231(2016 Nov)

?B-Crystallin overexpression in astrocytes modulates the phenotype of the BACHD mouse model of Huntington's disease.
Oliveira AO, Osmand A, Outeiro TF, Muchowski PJ, Finkbeiner S
Human molecular genetics25(9)1677-89(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In conclusion, CRYBB2 regulates expression of different lncRNAs to influence ovary development
Title: Long non-coding RNAs regulate effects of ?-crystallin B2 on mouse ovary development.

ovaries from female Crybb2(-/-) mice exhibited significantly reduced numbers of primordial, secondary and pre-ovulatory follicles when compared with WT mice, while the rate of atretic follicles was also increased
Title: Crybb2 deficiency impairs fertility in female mice.

BetaB2-crystallin has a role in hippocampal function and behavioral phenotypes.
Title: Crybb2 coding for ?B2-crystallin affects sensorimotor gating and hippocampal function.

The reduced fertility of Crybb2 knockout male mice may result from the disordered proliferation and apoptosis of germ cells in the testis, possibly due to reduced CaMKIV from the loss of Crybb2.
Title: Decreased levels of Ca??-calmodulin-dependent protein kinase IV in the testis as a contributing factor to reduced fertility in male Crybb2?/? mice.

BetaB2-crystallin is not essential for the normal development of a transparent lens in the mouse. It plays an increasingly important role in maintaining the transparency of the lens after birth.
Title: Targeted knockout of the mouse betaB2-crystallin gene (Crybb2) induces age-related cataract.

The following Crybb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Crybb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20436	NM_007773.4 Latest version!	Mus musculus crystallin, beta B2 (Crybb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu20436	XM_006534760.1 Latest version!	Mus musculus crystallin, beta B2 (Crybb2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu20436	XM_006534759.1 Latest version!	Mus musculus crystallin, beta B2 (Crybb2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu20436	XM_017320652.1 Latest version!	Mus musculus crystallin, beta B2 (Crybb2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu88724	XM_006534760.2 Latest version!	Mus musculus crystallin, beta B2 (Crybb2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Crybb2 ( NM_007773.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031799.1 Mus musculus crystallin, beta B2 (Crybb2), mRNA.

Price & Availability↑

CloneID	OMu20436
Clone ID Related Accession (Same CDS sequence)	XM_006534760.1 , NM_007773.4 , XM_017320652.1 , XM_006534759.1
Accession Version	NM_007773.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-31
Organism	Mus musculus(house mouse)
Product	beta-crystallin B2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC085185.1. On Feb 11, 2015 this sequence version replaced NM_007773.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC085185.1, CO427954.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849375, SAMN00849377 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCAG ACCACCAGAC ACAGGCGGGC AAGCCCCAGC CCCTTAACCC CAAGATCATC 
ATCTTCGAAC AGGAGAACTT CCAGGGCCAT TCCCACGAGC TCAGCGGGCC CTGCCCCAAC 
CTGAAGGAGA CTGGTATGGA GAAGGCGGGC TCCGTCCTGG TGCAGGCTGG ACCCTGGGTG 
GGCTACGAGC AGGCTAATTG CAAGGGCGAG CAGTTTGTGT TTGAAAAGGG CGAGTACCCA 
CGTTGGGACT CCTGGACCAG CAGCCGGAGG ACAGACTCCC TCAGCTCTCT GAGGCCCATC 
AAAGTGGACA GCCAAGAGCA CAAGATCATC TTATATGAGA ACCCCAACTT TACTGGCAAG 
AAGATGGAGA TTGTAGACGA CGATGTGCCC AGCTTCCATG CCCATGGATA CCAGGAAAAG 
GTGTCTTCCG TGCGTGTGCA GAGCGGCACG TGGGTGGGGT ACCAGTACCC TGGCTACCGT 
GGGCTGCAGT ACCTGCTGGA GAAGGGGGAT TACAAGGACA ACAGCGACTT CGGGGCCCCT 
CACCCCCAGG TGCAGTCTGT GCGTCGCATC CGTGACATGC AGTGGCACCA GCGAGGTGCC 
TTCCACCCCT CCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031799.1
CDS	197..814
Translation	MASDHQTQAGKPQPLNPKIIIFEQENFQGHSHELSGPCPNLKETGMEKAGSVLVQAGPWVGYEQANCKGEQFVFEKGEYPRWDSWTSSRRTDSLSSLRPIKVDSQEHKIILYENPNFTGKKMEIVDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPGYRGLQYLLEKGDYKDNSDFGAPHPQVQSVRRIRDMQWHQRGAFHPSS

Target ORF information:

RefSeq Version	NM_007773.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus crystallin, beta B2 (Crybb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007773.4

ORF Insert Sequence:

ATGGCCTCAG ACCACCAGAC ACAGGCGGGC AAGCCCCAGC CCCTTAACCC CAAGATCATC 
ATCTTCGAAC AGGAGAACTT CCAGGGCCAT TCCCACGAGC TCAGCGGGCC CTGCCCCAAC 
CTGAAGGAGA CTGGTATGGA GAAGGCGGGC TCCGTCCTGG TGCAGGCTGG ACCCTGGGTG 
GGCTACGAGC AGGCTAATTG CAAGGGCGAG CAGTTTGTGT TTGAAAAGGG CGAGTACCCA 
CGTTGGGACT CCTGGACCAG CAGCCGGAGG ACAGACTCCC TCAGCTCTCT GAGGCCCATC 
AAAGTGGACA GCCAAGAGCA CAAGATCATC TTATATGAGA ACCCCAACTT TACTGGCAAG 
AAGATGGAGA TTGTAGACGA CGATGTGCCC AGCTTCCATG CCCATGGATA CCAGGAAAAG 
GTGTCTTCCG TGCGTGTGCA GAGCGGCACG TGGGTGGGGT ACCAGTACCC TGGCTACCGT 
GGGCTGCAGT ACCTGCTGGA GAAGGGGGAT TACAAGGACA ACAGCGACTT CGGGGCCCCT 
CACCCCCAGG TGCAGTCTGT GCGTCGCATC CGTGACATGC AGTGGCACCA GCGAGGTGCC 
TTCCACCCCT CCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Crybb2 ( XM_006534760.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534823.1 Mus musculus crystallin, beta B2 (Crybb2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu20436
Clone ID Related Accession (Same CDS sequence)	XM_006534760.1 , NM_007773.4 , XM_017320652.1 , XM_006534759.1
Accession Version	XM_006534760.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	beta-crystallin B2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG ACCACCAGAC ACAGGCGGGC AAGCCCCAGC CCCTTAACCC CAAGATCATC 
ATCTTCGAAC AGGAGAACTT CCAGGGCCAT TCCCACGAGC TCAGCGGGCC CTGCCCCAAC 
CTGAAGGAGA CTGGTATGGA GAAGGCGGGC TCCGTCCTGG TGCAGGCTGG ACCCTGGGTG 
GGCTACGAGC AGGCTAATTG CAAGGGCGAG CAGTTTGTGT TTGAAAAGGG CGAGTACCCA 
CGTTGGGACT CCTGGACCAG CAGCCGGAGG ACAGACTCCC TCAGCTCTCT GAGGCCCATC 
AAAGTGGACA GCCAAGAGCA CAAGATCATC TTATATGAGA ACCCCAACTT TACTGGCAAG 
AAGATGGAGA TTGTAGACGA CGATGTGCCC AGCTTCCATG CCCATGGATA CCAGGAAAAG 
GTGTCTTCCG TGCGTGTGCA GAGCGGCACG TGGGTGGGGT ACCAGTACCC TGGCTACCGT 
GGGCTGCAGT ACCTGCTGGA GAAGGGGGAT TACAAGGACA ACAGCGACTT CGGGGCCCCT 
CACCCCCAGG TGCAGTCTGT GCGTCGCATC CGTGACATGC AGTGGCACCA GCGAGGTGCC 
TTCCACCCCT CCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534823.1
CDS	55..672
Translation	MASDHQTQAGKPQPLNPKIIIFEQENFQGHSHELSGPCPNLKETGMEKAGSVLVQAGPWVGYEQANCKGEQFVFEKGEYPRWDSWTSSRRTDSLSSLRPIKVDSQEHKIILYENPNFTGKKMEIVDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPGYRGLQYLLEKGDYKDNSDFGAPHPQVQSVRRIRDMQWHQRGAFHPSS

Target ORF information:

RefSeq Version	XM_006534760.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus crystallin, beta B2 (Crybb2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534760.1

ORF Insert Sequence:

ATGGCCTCAG ACCACCAGAC ACAGGCGGGC AAGCCCCAGC CCCTTAACCC CAAGATCATC 
ATCTTCGAAC AGGAGAACTT CCAGGGCCAT TCCCACGAGC TCAGCGGGCC CTGCCCCAAC 
CTGAAGGAGA CTGGTATGGA GAAGGCGGGC TCCGTCCTGG TGCAGGCTGG ACCCTGGGTG 
GGCTACGAGC AGGCTAATTG CAAGGGCGAG CAGTTTGTGT TTGAAAAGGG CGAGTACCCA 
CGTTGGGACT CCTGGACCAG CAGCCGGAGG ACAGACTCCC TCAGCTCTCT GAGGCCCATC 
AAAGTGGACA GCCAAGAGCA CAAGATCATC TTATATGAGA ACCCCAACTT TACTGGCAAG 
AAGATGGAGA TTGTAGACGA CGATGTGCCC AGCTTCCATG CCCATGGATA CCAGGAAAAG 
GTGTCTTCCG TGCGTGTGCA GAGCGGCACG TGGGTGGGGT ACCAGTACCC TGGCTACCGT 
GGGCTGCAGT ACCTGCTGGA GAAGGGGGAT TACAAGGACA ACAGCGACTT CGGGGCCCCT 
CACCCCCAGG TGCAGTCTGT GCGTCGCATC CGTGACATGC AGTGGCACCA GCGAGGTGCC 
TTCCACCCCT CCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Crybb2 ( XM_017320652.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176141.1 Mus musculus crystallin, beta B2 (Crybb2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu20436
Clone ID Related Accession (Same CDS sequence)	XM_006534760.1 , NM_007773.4 , XM_017320652.1 , XM_006534759.1
Accession Version	XM_017320652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	beta-crystallin B2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG ACCACCAGAC ACAGGCGGGC AAGCCCCAGC CCCTTAACCC CAAGATCATC 
ATCTTCGAAC AGGAGAACTT CCAGGGCCAT TCCCACGAGC TCAGCGGGCC CTGCCCCAAC 
CTGAAGGAGA CTGGTATGGA GAAGGCGGGC TCCGTCCTGG TGCAGGCTGG ACCCTGGGTG 
GGCTACGAGC AGGCTAATTG CAAGGGCGAG CAGTTTGTGT TTGAAAAGGG CGAGTACCCA 
CGTTGGGACT CCTGGACCAG CAGCCGGAGG ACAGACTCCC TCAGCTCTCT GAGGCCCATC 
AAAGTGGACA GCCAAGAGCA CAAGATCATC TTATATGAGA ACCCCAACTT TACTGGCAAG 
AAGATGGAGA TTGTAGACGA CGATGTGCCC AGCTTCCATG CCCATGGATA CCAGGAAAAG 
GTGTCTTCCG TGCGTGTGCA GAGCGGCACG TGGGTGGGGT ACCAGTACCC TGGCTACCGT 
GGGCTGCAGT ACCTGCTGGA GAAGGGGGAT TACAAGGACA ACAGCGACTT CGGGGCCCCT 
CACCCCCAGG TGCAGTCTGT GCGTCGCATC CGTGACATGC AGTGGCACCA GCGAGGTGCC 
TTCCACCCCT CCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176141.1
CDS	138..755
Translation	MASDHQTQAGKPQPLNPKIIIFEQENFQGHSHELSGPCPNLKETGMEKAGSVLVQAGPWVGYEQANCKGEQFVFEKGEYPRWDSWTSSRRTDSLSSLRPIKVDSQEHKIILYENPNFTGKKMEIVDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPGYRGLQYLLEKGDYKDNSDFGAPHPQVQSVRRIRDMQWHQRGAFHPSS

Target ORF information:

RefSeq Version	XM_017320652.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus crystallin, beta B2 (Crybb2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320652.1

ORF Insert Sequence:

ATGGCCTCAG ACCACCAGAC ACAGGCGGGC AAGCCCCAGC CCCTTAACCC CAAGATCATC 
ATCTTCGAAC AGGAGAACTT CCAGGGCCAT TCCCACGAGC TCAGCGGGCC CTGCCCCAAC 
CTGAAGGAGA CTGGTATGGA GAAGGCGGGC TCCGTCCTGG TGCAGGCTGG ACCCTGGGTG 
GGCTACGAGC AGGCTAATTG CAAGGGCGAG CAGTTTGTGT TTGAAAAGGG CGAGTACCCA 
CGTTGGGACT CCTGGACCAG CAGCCGGAGG ACAGACTCCC TCAGCTCTCT GAGGCCCATC 
AAAGTGGACA GCCAAGAGCA CAAGATCATC TTATATGAGA ACCCCAACTT TACTGGCAAG 
AAGATGGAGA TTGTAGACGA CGATGTGCCC AGCTTCCATG CCCATGGATA CCAGGAAAAG 
GTGTCTTCCG TGCGTGTGCA GAGCGGCACG TGGGTGGGGT ACCAGTACCC TGGCTACCGT 
GGGCTGCAGT ACCTGCTGGA GAAGGGGGAT TACAAGGACA ACAGCGACTT CGGGGCCCCT 
CACCCCCAGG TGCAGTCTGT GCGTCGCATC CGTGACATGC AGTGGCACCA GCGAGGTGCC 
TTCCACCCCT CCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Crybb2 ( XM_006534759.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534822.1 Mus musculus crystallin, beta B2 (Crybb2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu20436
Clone ID Related Accession (Same CDS sequence)	XM_006534760.1 , NM_007773.4 , XM_017320652.1 , XM_006534759.1
Accession Version	XM_006534759.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	beta-crystallin B2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG ACCACCAGAC ACAGGCGGGC AAGCCCCAGC CCCTTAACCC CAAGATCATC 
ATCTTCGAAC AGGAGAACTT CCAGGGCCAT TCCCACGAGC TCAGCGGGCC CTGCCCCAAC 
CTGAAGGAGA CTGGTATGGA GAAGGCGGGC TCCGTCCTGG TGCAGGCTGG ACCCTGGGTG 
GGCTACGAGC AGGCTAATTG CAAGGGCGAG CAGTTTGTGT TTGAAAAGGG CGAGTACCCA 
CGTTGGGACT CCTGGACCAG CAGCCGGAGG ACAGACTCCC TCAGCTCTCT GAGGCCCATC 
AAAGTGGACA GCCAAGAGCA CAAGATCATC TTATATGAGA ACCCCAACTT TACTGGCAAG 
AAGATGGAGA TTGTAGACGA CGATGTGCCC AGCTTCCATG CCCATGGATA CCAGGAAAAG 
GTGTCTTCCG TGCGTGTGCA GAGCGGCACG TGGGTGGGGT ACCAGTACCC TGGCTACCGT 
GGGCTGCAGT ACCTGCTGGA GAAGGGGGAT TACAAGGACA ACAGCGACTT CGGGGCCCCT 
CACCCCCAGG TGCAGTCTGT GCGTCGCATC CGTGACATGC AGTGGCACCA GCGAGGTGCC 
TTCCACCCCT CCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534822.1
CDS	102..719
Translation	MASDHQTQAGKPQPLNPKIIIFEQENFQGHSHELSGPCPNLKETGMEKAGSVLVQAGPWVGYEQANCKGEQFVFEKGEYPRWDSWTSSRRTDSLSSLRPIKVDSQEHKIILYENPNFTGKKMEIVDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPGYRGLQYLLEKGDYKDNSDFGAPHPQVQSVRRIRDMQWHQRGAFHPSS

Target ORF information:

RefSeq Version	XM_006534759.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus crystallin, beta B2 (Crybb2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534759.1

ORF Insert Sequence:

ATGGCCTCAG ACCACCAGAC ACAGGCGGGC AAGCCCCAGC CCCTTAACCC CAAGATCATC 
ATCTTCGAAC AGGAGAACTT CCAGGGCCAT TCCCACGAGC TCAGCGGGCC CTGCCCCAAC 
CTGAAGGAGA CTGGTATGGA GAAGGCGGGC TCCGTCCTGG TGCAGGCTGG ACCCTGGGTG 
GGCTACGAGC AGGCTAATTG CAAGGGCGAG CAGTTTGTGT TTGAAAAGGG CGAGTACCCA 
CGTTGGGACT CCTGGACCAG CAGCCGGAGG ACAGACTCCC TCAGCTCTCT GAGGCCCATC 
AAAGTGGACA GCCAAGAGCA CAAGATCATC TTATATGAGA ACCCCAACTT TACTGGCAAG 
AAGATGGAGA TTGTAGACGA CGATGTGCCC AGCTTCCATG CCCATGGATA CCAGGAAAAG 
GTGTCTTCCG TGCGTGTGCA GAGCGGCACG TGGGTGGGGT ACCAGTACCC TGGCTACCGT 
GGGCTGCAGT ACCTGCTGGA GAAGGGGGAT TACAAGGACA ACAGCGACTT CGGGGCCCCT 
CACCCCCAGG TGCAGTCTGT GCGTCGCATC CGTGACATGC AGTGGCACCA GCGAGGTGCC 
TTCCACCCCT CCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Crybb2 ( XM_006534760.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534823.2 Mus musculus crystallin, beta B2 (Crybb2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu88724
Clone ID Related Accession (Same CDS sequence)	XM_006534760.2
Accession Version	XM_006534760.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	beta-crystallin B2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006534760.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAC AAGCACTTGG GCTAGTTCAA CTGAGTCCAG CACCTAAAGG TAGAGAGATT 
GGAGAGCTGG AAGTGGCCTC GGGGAAGCCT TCCGGCATGA CTCCACGGGG CTGGAGAGAG 
ACCTTGGGAG AGCTGGGATT CATCTGCTCA GGGCTGAACT GGATTCATAC TGAAGGTCAC 
CTCGACGCCA GAGAGTCCAC CATGGCCTCA GACCACCAGA CACAGGCGGG CAAGCCCCAG 
CCCCTTAACC CCAAGATCAT CATCTTCGAA CAGGAGAACT TCCAGGGCCA TTCCCACGAG 
CTCAGCGGGC CCTGCCCCAA CCTGAAGGAG ACTGGTATGG AGAAGGCGGG CTCCGTCCTG 
GTGCAGGCTG GACCCTGGGT GGGCTACGAG CAGGCTAATT GCAAGGGCGA GCAGTTTGTG 
TTTGAAAAGG GCGAGTACCC ACGTTGGGAC TCCTGGACCA GCAGCCGGAG GACAGACTCC 
CTCAGCTCTC TGAGGCCCAT CAAAGTGGAC AGCCAAGAGC ACAAGATCAT CTTATATGAG 
AACCCCAACT TTACTGGCAA GAAGATGGAG ATTGTAGACG ACGATGTGCC CAGCTTCCAT 
GCCCATGGAT ACCAGGAAAA GGTGTCTTCC GTGCGTGTGC AGAGCGGCAC GTGGGTGGGG 
TACCAGTACC CTGGCTACCG TGGGCTGCAG TACCTGCTGG AGAAGGGGGA TTACAAGGAC 
AACAGCGACT TCGGGGCCCC TCACCCCCAG GTGCAGTCTG TGCGTCGCAT CCGTGACATG 
CAGTGGCACC AGCGAGGTGC CTTCCACCCC TCCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534823.2
CDS	399..1217
Translation	MAEQALGLVQLSPAPKGREIGELEVASGKPSGMTPRGWRETLGELGFICSGLNWIHTEGHLDARESTMASDHQTQAGKPQPLNPKIIIFEQENFQGHSHELSGPCPNLKETGMEKAGSVLVQAGPWVGYEQANCKGEQFVFEKGEYPRWDSWTSSRRTDSLSSLRPIKVDSQEHKIILYENPNFTGKKMEIVDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPGYRGLQYLLEKGDYKDNSDFGAPHPQVQSVRRIRDMQWHQRGAFHPSS

Target ORF information:

RefSeq Version	XM_006534760.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus crystallin, beta B2 (Crybb2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534760.2

ORF Insert Sequence:

ATGGCAGAAC AAGCACTTGG GCTAGTTCAA CTGAGTCCAG CACCTAAAGG TAGAGAGATT 
GGAGAGCTGG AAGTGGCCTC GGGGAAGCCT TCCGGCATGA CTCCACGGGG CTGGAGAGAG 
ACCTTGGGAG AGCTGGGATT CATCTGCTCA GGGCTGAACT GGATTCATAC TGAAGGTCAC 
CTCGACGCCA GAGAGTCCAC CATGGCCTCA GACCACCAGA CACAGGCGGG CAAGCCCCAG 
CCCCTTAACC CCAAGATCAT CATCTTCGAA CAGGAGAACT TCCAGGGCCA TTCCCACGAG 
CTCAGCGGGC CCTGCCCCAA CCTGAAGGAG ACTGGTATGG AGAAGGCGGG CTCCGTCCTG 
GTGCAGGCTG GACCCTGGGT GGGCTACGAG CAGGCTAATT GCAAGGGCGA GCAGTTTGTG 
TTTGAAAAGG GCGAGTACCC ACGTTGGGAC TCCTGGACCA GCAGCCGGAG GACAGACTCC 
CTCAGCTCTC TGAGGCCCAT CAAAGTGGAC AGCCAAGAGC ACAAGATCAT CTTATATGAG 
AACCCCAACT TTACTGGCAA GAAGATGGAG ATTGTAGACG ACGATGTGCC CAGCTTCCAT 
GCCCATGGAT ACCAGGAAAA GGTGTCTTCC GTGCGTGTGC AGAGCGGCAC GTGGGTGGGG 
TACCAGTACC CTGGCTACCG TGGGCTGCAG TACCTGCTGG AGAAGGGGGA TTACAAGGAC 
AACAGCGACT TCGGGGCCCC TCACCCCCAG GTGCAGTCTG TGCGTCGCAT CCGTGACATG 
CAGTGGCACC AGCGAGGTGC CTTCCACCCC TCCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

N-cadherin regulates signaling mechanisms required for lens fiber cell elongation and lens morphogenesis.
Developmental biology428(1)118-134(2017 08)
Logan CM,Rajakaruna S,Bowen C,Radice GL,Robinson ML,Menko AS

Long non-coding RNAs regulate effects of ?-crystallin B2 on mouse ovary development.
Molecular medicine reports14(5)4223-4231(2016 Nov)
Gao Q,Ren H,Chen M,Niu Z,Tao H,Jia Y,Zhang J,Li W

?B-Crystallin overexpression in astrocytes modulates the phenotype of the BACHD mouse model of Huntington's disease.
Human molecular genetics25(9)1677-89(2016 05)
Oliveira AO,Osmand A,Outeiro TF,Muchowski PJ,Finkbeiner S

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Crybb2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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