Cdc25a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cdc25a
Entrez Gene ID	12530
Full Name	cell division cycle 25A
Synonyms	D9Ertd393e
General protein information	Preferred Names cell division cycle 25A Names M-phase inducer phosphatase 1 dual specificity phosphatase Cdc25A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 F2\|9 59.81 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006511929.3	XP_006511992.1	M-phase inducer phosphatase 1 isoform X1
NM_007658.3	NP_031684.3	M-phase inducer phosphatase 1
XM_006511931.4	XP_006511994.1	M-phase inducer phosphatase 1 isoform X3
XM_006511931.3	XP_006511994.1	M-phase inducer phosphatase 1 isoform X3
XM_006511930.3	XP_006511993.1	M-phase inducer phosphatase 1 isoform X2
XM_006511929.4	XP_006511992.1	M-phase inducer phosphatase 1 isoform X1
XM_006511930.4	XP_006511993.1	M-phase inducer phosphatase 1 isoform X2

Pathways from BioSystems

Homologies

	CDC25A	XP_002802898.1
Canis lupus familiaris (dog)	CDC25A	NP_001138687.1
Xenopus tropicalis (tropical clawed frog)	cdc25a	NP_001039063.2
Pan troglodytes (chimpanzee)	CDC25A	XP_516433.2
Gallus gallus (chicken)	CDC25A	NP_001186501.1
Homo sapiens (human)	CDC25A	NP_001780.2
Bos taurus (cattle)	CDC25A	NP_001094570.1
Mus musculus (house mouse)	Cdc25a	NP_031684.3
Rattus norvegicus (Norway rat)	Cdc25a	NP_598255.1
Danio rerio (zebrafish)	cdc25	NP_001108567.1

Gene Ontology
Bibliography

Related articles in PubMed

Cell cycle-targeting microRNAs promote differentiation by enforcing cell-cycle exit.
Otto T, Candido SV, Pilarz MS, Sicinska E, Bronson RT, Bowden M, Lachowicz IA, Mulry K, Fassl A, Han RC, Jecrois ES, Sicinski P
Proceedings of the National Academy of Sciences of the United States of America114(40)10660-10665(2017 Oct)

Cdc25A Is a Critical Mediator of Ischemic Neuronal Death
Iyirhiaro GO, Im DS, Boonying W, Callaghan SM, During MJ, Slack RS, Park DS
The Journal of neuroscience : the official journal of the Society for Neuroscience37(28)6729-6740(2017 Jul)

A Genome-wide CRISPR Screen Identifies CDC25A as a Determinant of Sensitivity to ATR Inhibitors.
Ruiz S, Mayor-Ruiz C, Lafarga V, Murga M, Vega-Sendino M, Ortega S, Fernandez-Capetillo O
Molecular cell62(2)307-13(2016 04)

Oocyte-expressed yes-associated protein is a key activator of the early zygotic genome in mouse.
Yu C, Ji SY, Dang YJ, Sha QQ, Yuan YF, Zhou JJ, Yan LY, Qiao J, Tang F, Fan HY
Cell research26(3)275-87(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results identify Cdc25A as a potential target for neuroprotectant strategy for the treatment of delayed ischemic neuronal death.
Title: Cdc25A Is a Critical Mediator of Ischemic Neuronal Death

CDC25A-deficient embryonic stem cells resist high doses of ATR inhibitors, which we show is due to their failure to prematurely enter mitosis in response to the drugs.
Title: A Genome-wide CRISPR Screen Identifies CDC25A as a Determinant of Sensitivity to ATR Inhibitors.

These results showed that IGF1 regulated the expression of BOULE and CDC25A mRNAs via ERK1/2 signaling and in T-independent pathway during spermatogenesis in the adult mouse testes.
Title: IGF1 regulation of BOULE and CDC25A transcripts via a testosterone-independent pathway in spermatogenesis of adult mice.

accelerated cholangiocyte cystogenesis is likely due to overexpression of Cdc25A
Title: Centrosomal abnormalities characterize human and rodent cystic cholangiocytes and are associated with Cdc25A overexpression.

Cdc25A activity is required for the metaphase II arrest in mouse oocytes.
Title: Cdc25A activity is required for the metaphase II arrest in mouse oocytes.

The following Cdc25a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cdc25a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15083	NM_007658.3 Latest version!	Mus musculus cell division cycle 25A (Cdc25a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00
OMu43288	XM_006511929.4 Latest version!	Mus musculus cell division cycle 25A (Cdc25a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu43289	XM_006511930.4 Latest version!	Mus musculus cell division cycle 25A (Cdc25a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu43290	XM_006511931.4 Latest version!	Mus musculus cell division cycle 25A (Cdc25a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu43290	XM_006511931.3	Mus musculus cell division cycle 25A (Cdc25a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu43288	XM_006511929.3	Mus musculus cell division cycle 25A (Cdc25a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu43289	XM_006511930.3	Mus musculus cell division cycle 25A (Cdc25a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cdc25a ( NM_007658.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031684.3 Mus musculus cell division cycle 25A (Cdc25a), mRNA.

Price & Availability↑

CloneID	OMu15083
Clone ID Related Accession (Same CDS sequence)	NM_007658.3
Accession Version	NM_007658.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-10
Organism	Mus musculus(house mouse)
Product	M-phase inducer phosphatase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK077991.1, AK077609.1, BE854932.1 and BU700379.1. On Apr 18, 2006 this sequence version replaced NM_007658.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK077991.1, AK077609.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849378 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACTGG GCCCGGAGCC CCCCCACCGC CGCCGCCTGT TCTTCGCTTG CAGCCCCACG 
CCTGCGCCGC AGCCCACGGG GAAGATGCTG TTTGGCGCGT CAGCTGCTGG CGGACTGTCC 
CCTGTCACCA ACCTGACGGT CACCATGGAC CAGCTGGAAG GACTGGGCAG TGACTGTGAG 
AAAATGGAAG TGAGAAATAA CAGCAGTCTA CAGAGAATGG GCTCCTCAGA ATCGACCGAT 
TCAGGTTTCT GTCTAGATTC TCCTGGGCCC TTGGACAGTA AAGAAAACCT TGAAATTTCC 
CTGACGAGAA TAAATTCCCT ACCTCAGAAG CTCCTGGGAT GTAGCCCAGC CCTGAAGAGG 
AGCCACTCTG ATTCTCTAGA CCACGACACC TTTCACCTCA TCGACCAGGA TGAAAATAAA 
GAAAATGAAG CATTTGAATT TAAAAAGCCA ATACGACCTG CATCTCGTCA CATCTACGAG 
GAGAGTAAAG ATCCCTTTAC ACACAGGCAG AATTCAGCCC CAGCTCGGAT GCTATCTTCA 
AATGAAAGTG AATCAGGAAA TTTCAGTCCT CTTTTTATAC CCCAGTCACC TGTAAAAGCC 
ACTTTGTCCG ATGAGGATGA TGGCTTCATA GACCTTCTGG ATGGCGAGAA TATGAAGAAT 
GATGAGGAGA CCCCATCATG CATGGCAAGC CTCTGGACAG CTCCCCTTGT CATGAGGAGA 
CCTGCAAACC TTGCCGATCG TTGCGGGCTG TTTGACTCCC CTTCCCCGTG CGGCTCCAGC 
ACTCGGGCGG TGTTGAAGAG AGCAGACCGG TCTCACGAGG AGCCTCCTCG GGGTACAAAG 
AGGAGGAAGA GTGTGCCCAG CCCTGTGAAG GCGAAGGCGG ATGTTCCGGA GCCCGCCCAG 
CTTCCATCCC AGTCTCTATC CCTGATGTCT TCCCCCAAAG GAACCATTGA GAACATTTTG 
GACAGTGACC CAAGAGACCT TATAGGAGAT TTCTCCAAGG GTTATCTCTT TAATACAGTC 
TCTGGGAAGC ATCAGGATTT GAAATATATT TCTCCAGAAA TTATGGCATC TGTTTTGAAT 
GGCAAGTTTG CCGGTCTCAT TAAAGAGTTT GTTATCATTG ACTGCCGATA CCCATATGAG 
TATGAAGGAG GTCACATCAA GGGTGCCGTG AATCTGCACA TGGAAGAAGA GGTTGAAGAC 
TTCTTACTTA AGAACCCTAT TGTGCCTACT GATGGCAAGC GTGTCATTGT CGTGTTCCAC 
TGTGAGTTTT CCTCTGAAAG AGGCCCTCGA ATGTGCCGAT ATGTGAGAGA GAGAGATAGG 
CTCGGCAATG AGTACCCCAA GCTCCACTAC CCTGAGCTGT ATGTCCTGAA GGGGGGATAC 
AAGGAGTTCT TTTTGAAGTG CCAGTCTCAC TGTGAACCCC CCAGCTACCG GCCAATGCAC 
CATGAAGACT TTAAAGAAGA CCTAAAGAAG TTCCGCACCA AGAGCCGGAC CTGGGCAGGA 
GAAAAGAGCA AAAGAGAGAT GTACAGTCGC CTGAAGAAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031684.3
CDS	412..1956
Translation	MELGPEPPHRRRLFFACSPTPAPQPTGKMLFGASAAGGLSPVTNLTVTMDQLEGLGSDCEKMEVRNNSSLQRMGSSESTDSGFCLDSPGPLDSKENLEISLTRINSLPQKLLGCSPALKRSHSDSLDHDTFHLIDQDENKENEAFEFKKPIRPASRHIYEESKDPFTHRQNSAPARMLSSNESESGNFSPLFIPQSPVKATLSDEDDGFIDLLDGENMKNDEETPSCMASLWTAPLVMRRPANLADRCGLFDSPSPCGSSTRAVLKRADRSHEEPPRGTKRRKSVPSPVKAKADVPEPAQLPSQSLSLMSSPKGTIENILDSDPRDLIGDFSKGYLFNTVSGKHQDLKYISPEIMASVLNGKFAGLIKEFVIIDCRYPYEYEGGHIKGAVNLHMEEEVEDFLLKNPIVPTDGKRVIVVFHCEFSSERGPRMCRYVRERDRLGNEYPKLHYPELYVLKGGYKEFFLKCQSHCEPPSYRPMHHEDFKEDLKKFRTKSRTWAGEKSKREMYSRLKKL

Target ORF information:

RefSeq Version	NM_007658.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cell division cycle 25A (Cdc25a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007658.3

ORF Insert Sequence:

ATGGAACTGG GCCCGGAGCC CCCCCACCGC CGCCGCCTGT TCTTCGCTTG CAGCCCCACG 
CCTGCGCCGC AGCCCACGGG GAAGATGCTG TTTGGCGCGT CAGCTGCTGG CGGACTGTCC 
CCTGTCACCA ACCTGACGGT CACCATGGAC CAGCTGGAAG GACTGGGCAG TGACTGTGAG 
AAAATGGAAG TGAGAAATAA CAGCAGTCTA CAGAGAATGG GCTCCTCAGA ATCGACCGAT 
TCAGGTTTCT GTCTAGATTC TCCTGGGCCC TTGGACAGTA AAGAAAACCT TGAAATTTCC 
CTGACGAGAA TAAATTCCCT ACCTCAGAAG CTCCTGGGAT GTAGCCCAGC CCTGAAGAGG 
AGCCACTCTG ATTCTCTAGA CCACGACACC TTTCACCTCA TCGACCAGGA TGAAAATAAA 
GAAAATGAAG CATTTGAATT TAAAAAGCCA ATACGACCTG CATCTCGTCA CATCTACGAG 
GAGAGTAAAG ATCCCTTTAC ACACAGGCAG AATTCAGCCC CAGCTCGGAT GCTATCTTCA 
AATGAAAGTG AATCAGGAAA TTTCAGTCCT CTTTTTATAC CCCAGTCACC TGTAAAAGCC 
ACTTTGTCCG ATGAGGATGA TGGCTTCATA GACCTTCTGG ATGGCGAGAA TATGAAGAAT 
GATGAGGAGA CCCCATCATG CATGGCAAGC CTCTGGACAG CTCCCCTTGT CATGAGGAGA 
CCTGCAAACC TTGCCGATCG TTGCGGGCTG TTTGACTCCC CTTCCCCGTG CGGCTCCAGC 
ACTCGGGCGG TGTTGAAGAG AGCAGACCGG TCTCACGAGG AGCCTCCTCG GGGTACAAAG 
AGGAGGAAGA GTGTGCCCAG CCCTGTGAAG GCGAAGGCGG ATGTTCCGGA GCCCGCCCAG 
CTTCCATCCC AGTCTCTATC CCTGATGTCT TCCCCCAAAG GAACCATTGA GAACATTTTG 
GACAGTGACC CAAGAGACCT TATAGGAGAT TTCTCCAAGG GTTATCTCTT TAATACAGTC 
TCTGGGAAGC ATCAGGATTT GAAATATATT TCTCCAGAAA TTATGGCATC TGTTTTGAAT 
GGCAAGTTTG CCGGTCTCAT TAAAGAGTTT GTTATCATTG ACTGCCGATA CCCATATGAG 
TATGAAGGAG GTCACATCAA GGGTGCCGTG AATCTGCACA TGGAAGAAGA GGTTGAAGAC 
TTCTTACTTA AGAACCCTAT TGTGCCTACT GATGGCAAGC GTGTCATTGT CGTGTTCCAC 
TGTGAGTTTT CCTCTGAAAG AGGCCCTCGA ATGTGCCGAT ATGTGAGAGA GAGAGATAGG 
CTCGGCAATG AGTACCCCAA GCTCCACTAC CCTGAGCTGT ATGTCCTGAA GGGGGGATAC 
AAGGAGTTCT TTTTGAAGTG CCAGTCTCAC TGTGAACCCC CCAGCTACCG GCCAATGCAC 
CATGAAGACT TTAAAGAAGA CCTAAAGAAG TTCCGCACCA AGAGCCGGAC CTGGGCAGGA 
GAAAAGAGCA AAAGAGAGAT GTACAGTCGC CTGAAGAAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc25a ( XM_006511929.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511992.1 Mus musculus cell division cycle 25A (Cdc25a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu43288
Clone ID Related Accession (Same CDS sequence)	XM_006511929.3 , XM_006511929.4
Accession Version	XM_006511929.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	M-phase inducer phosphatase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006511929.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGG GCCCGGAGCC CCCCCACCGC CGCCGCCTGT TCTTCGCTTG CAGCCCCACG 
CCTGCGCCGC AGCCCACGGG GAAGATGCTG TTTGGCGCGT CAGCTGCTGG CGGACTGTCC 
CCTGTCACCA ACCTGACGGT CACCATGGAC CAGCTGGAAG GACTGGGCAG TGACTGTGAG 
AAAATGGAAG TGAGAAATAA CAGCAGTCTA CAGAGAATGG GCTCCTCAGA ATCGACCGAT 
TCAGGTTTCT GTCTAGATTC TCCTGGGCCC TTGGACAGTA AAGAAAACCT TGAAATTTCC 
CTGACGAGAA TAAATTCCCT ACCTCAGAAG CTCCTGGGAT GTAGCCCAGC CCTGAAGAGG 
AGCCACTCTG ATTCTCTAGA CCACGACACC TTTCACCTCA TCGACCAGGA TGAAAATAAA 
GAAAATGAAG CATTTGAATT TAAAAAGCCA ATACGACCTG CATCTCGTCA CATCTACGAG 
GAGAGTAAAG ATCCCTTTAC ACACAGGCAG AATTCAGCCC CAGCTCGGAT GCTATCTTCA 
AATGAAAGTG AATCAGGAAA TTTCAGTCCT CTTTTTATAC CCCAGTCACC TGTAAAAGCC 
ACTTTGTCCG ATGAGGATGA TGGCTTCATA GACCTTCTGG ATGGCGAGAA TATGAAGAAT 
GATGAGGAGA CCCCATCATG CATGGCAAGC CTCTGGACAG CTCCCCTTGT CATGAGGAGA 
CCTGCAAACC TTGTTGGACC TGTCTCTGAC CCTAAGGCCG ATCGTTGCGG GCTGTTTGAC 
TCCCCTTCCC CGTGCGGCTC CAGCACTCGG GCGGTGTTGA AGAGAGCAGA CCGGTCTCAC 
GAGGAGCCTC CTCGGGGTAC AAAGAGGAGG AAGAGTGTGC CCAGCCCTGT GAAGGCGAAG 
GCGGATGTTC CGGAGCCCGC CCAGCTTCCA TCCCAGTCTC TATCCCTGAT GTCTTCCCCC 
AAAGGAACCA TTGAGAACAT TTTGGACAGT GACCCAAGAG ACCTTATAGG AGATTTCTCC 
AAGGGTTATC TCTTTAATAC AGTCTCTGGG AAGCATCAGG ATTTGAAATA TATTTCTCCA 
GAAATTATGG CATCTGTTTT GAATGGCAAG TTTGCCGGTC TCATTAAAGA GTTTGTTATC 
ATTGACTGCC GATACCCATA TGAGTATGAA GGAGGTCACA TCAAGGGTGC CGTGAATCTG 
CACATGGAAG AAGAGGTTGA AGACTTCTTA CTTAAGAACC CTATTGTGCC TACTGATGGC 
AAGCGTGTCA TTGTCGTGTT CCACTGTGAG TTTTCCTCTG AAAGAGGCCC TCGAATGTGC 
CGATATGTGA GAGAGAGAGA TAGGCTCGGC AATGAGTACC CCAAGCTCCA CTACCCTGAG 
CTGTATGTCC TGAAGGGGGG ATACAAGGAG TTCTTTTTGA AGTGCCAGTC TCACTGTGAA 
CCCCCCAGCT ACCGGCCAAT GCACCATGAA GACTTTAAAG AAGACCTAAA GAAGTTCCGC 
ACCAAGAGCC GGACCTGGGC AGGAGAAAAG AGCAAAAGAG AGATGTACAG TCGCCTGAAG 
AAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511992.1
CDS	656..2224
Translation	MELGPEPPHRRRLFFACSPTPAPQPTGKMLFGASAAGGLSPVTNLTVTMDQLEGLGSDCEKMEVRNNSSLQRMGSSESTDSGFCLDSPGPLDSKENLEISLTRINSLPQKLLGCSPALKRSHSDSLDHDTFHLIDQDENKENEAFEFKKPIRPASRHIYEESKDPFTHRQNSAPARMLSSNESESGNFSPLFIPQSPVKATLSDEDDGFIDLLDGENMKNDEETPSCMASLWTAPLVMRRPANLVGPVSDPKADRCGLFDSPSPCGSSTRAVLKRADRSHEEPPRGTKRRKSVPSPVKAKADVPEPAQLPSQSLSLMSSPKGTIENILDSDPRDLIGDFSKGYLFNTVSGKHQDLKYISPEIMASVLNGKFAGLIKEFVIIDCRYPYEYEGGHIKGAVNLHMEEEVEDFLLKNPIVPTDGKRVIVVFHCEFSSERGPRMCRYVRERDRLGNEYPKLHYPELYVLKGGYKEFFLKCQSHCEPPSYRPMHHEDFKEDLKKFRTKSRTWAGEKSKREMYSRLKKL

Target ORF information:

RefSeq Version	XM_006511929.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cell division cycle 25A (Cdc25a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511929.3

ORF Insert Sequence:

ATGGAACTGG GCCCGGAGCC CCCCCACCGC CGCCGCCTGT TCTTCGCTTG CAGCCCCACG 
CCTGCGCCGC AGCCCACGGG GAAGATGCTG TTTGGCGCGT CAGCTGCTGG CGGACTGTCC 
CCTGTCACCA ACCTGACGGT CACCATGGAC CAGCTGGAAG GACTGGGCAG TGACTGTGAG 
AAAATGGAAG TGAGAAATAA CAGCAGTCTA CAGAGAATGG GCTCCTCAGA ATCGACCGAT 
TCAGGTTTCT GTCTAGATTC TCCTGGGCCC TTGGACAGTA AAGAAAACCT TGAAATTTCC 
CTGACGAGAA TAAATTCCCT ACCTCAGAAG CTCCTGGGAT GTAGCCCAGC CCTGAAGAGG 
AGCCACTCTG ATTCTCTAGA CCACGACACC TTTCACCTCA TCGACCAGGA TGAAAATAAA 
GAAAATGAAG CATTTGAATT TAAAAAGCCA ATACGACCTG CATCTCGTCA CATCTACGAG 
GAGAGTAAAG ATCCCTTTAC ACACAGGCAG AATTCAGCCC CAGCTCGGAT GCTATCTTCA 
AATGAAAGTG AATCAGGAAA TTTCAGTCCT CTTTTTATAC CCCAGTCACC TGTAAAAGCC 
ACTTTGTCCG ATGAGGATGA TGGCTTCATA GACCTTCTGG ATGGCGAGAA TATGAAGAAT 
GATGAGGAGA CCCCATCATG CATGGCAAGC CTCTGGACAG CTCCCCTTGT CATGAGGAGA 
CCTGCAAACC TTGTTGGACC TGTCTCTGAC CCTAAGGCCG ATCGTTGCGG GCTGTTTGAC 
TCCCCTTCCC CGTGCGGCTC CAGCACTCGG GCGGTGTTGA AGAGAGCAGA CCGGTCTCAC 
GAGGAGCCTC CTCGGGGTAC AAAGAGGAGG AAGAGTGTGC CCAGCCCTGT GAAGGCGAAG 
GCGGATGTTC CGGAGCCCGC CCAGCTTCCA TCCCAGTCTC TATCCCTGAT GTCTTCCCCC 
AAAGGAACCA TTGAGAACAT TTTGGACAGT GACCCAAGAG ACCTTATAGG AGATTTCTCC 
AAGGGTTATC TCTTTAATAC AGTCTCTGGG AAGCATCAGG ATTTGAAATA TATTTCTCCA 
GAAATTATGG CATCTGTTTT GAATGGCAAG TTTGCCGGTC TCATTAAAGA GTTTGTTATC 
ATTGACTGCC GATACCCATA TGAGTATGAA GGAGGTCACA TCAAGGGTGC CGTGAATCTG 
CACATGGAAG AAGAGGTTGA AGACTTCTTA CTTAAGAACC CTATTGTGCC TACTGATGGC 
AAGCGTGTCA TTGTCGTGTT CCACTGTGAG TTTTCCTCTG AAAGAGGCCC TCGAATGTGC 
CGATATGTGA GAGAGAGAGA TAGGCTCGGC AATGAGTACC CCAAGCTCCA CTACCCTGAG 
CTGTATGTCC TGAAGGGGGG ATACAAGGAG TTCTTTTTGA AGTGCCAGTC TCACTGTGAA 
CCCCCCAGCT ACCGGCCAAT GCACCATGAA GACTTTAAAG AAGACCTAAA GAAGTTCCGC 
ACCAAGAGCC GGACCTGGGC AGGAGAAAAG AGCAAAAGAG AGATGTACAG TCGCCTGAAG 
AAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc25a ( XM_006511930.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511993.1 Mus musculus cell division cycle 25A (Cdc25a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu43289
Clone ID Related Accession (Same CDS sequence)	XM_006511930.3 , XM_006511930.4
Accession Version	XM_006511930.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	M-phase inducer phosphatase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006511930.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGG GCCCGGAGCC CCCCCACCGC CGCCGCCTGT TCTTCGCTTG CAGCCCCACG 
CCTGCGCCGC AGCCCACGGG GAAGATGCTG TTTGGCGCGT CAGCTGCTGG CGGACTGTCC 
CCTGTCACCA ACCTGACGGT CACCATGGAC CAGCTGGAAG GACTGGGCAG TGACTGTGAG 
AAAATGGAAG TGAGAAATAA CAGCAGTCTA CAGAGAATGG GCTCCTCAGA ATCGACCGAT 
TCAGGTTTCT GTCTAGATTC TCCTGGGCCC TTGGACAGTA AAGAAAACCT TGAAATTTCC 
CTGACGAGAA TAAATTCCCT ACCTAAGCTC CTGGGATGTA GCCCAGCCCT GAAGAGGAGC 
CACTCTGATT CTCTAGACCA CGACACCTTT CACCTCATCG ACCAGGATGA AAATAAAGAA 
AATGAAGCAT TTGAATTTAA AAAGCCAATA CGACCTGCAT CTCGTCACAT CTACGAGGAG 
AGTAAAGATC CCTTTACACA CAGGCAGAAT TCAGCCCCAG CTCGGATGCT ATCTTCAAAT 
GAAAGTGAAT CAGGAAATTT CAGTCCTCTT TTTATACCCC AGTCACCTGT AAAAGCCACT 
TTGTCCGATG AGGATGATGG CTTCATAGAC CTTCTGGATG GCGAGAATAT GAAGAATGAT 
GAGGAGACCC CATCATGCAT GGCAAGCCTC TGGACAGCTC CCCTTGTCAT GAGGAGACCT 
GCAAACCTTG TTGGACCTGT CTCTGACCCT AAGGCCGATC GTTGCGGGCT GTTTGACTCC 
CCTTCCCCGT GCGGCTCCAG CACTCGGGCG GTGTTGAAGA GAGCAGACCG GTCTCACGAG 
GAGCCTCCTC GGGGTACAAA GAGGAGGAAG AGTGTGCCCA GCCCTGTGAA GGCGAAGGCG 
GATGTTCCGG AGCCCGCCCA GCTTCCATCC CAGTCTCTAT CCCTGATGTC TTCCCCCAAA 
GGAACCATTG AGAACATTTT GGACAGTGAC CCAAGAGACC TTATAGGAGA TTTCTCCAAG 
GGTTATCTCT TTAATACAGT CTCTGGGAAG CATCAGGATT TGAAATATAT TTCTCCAGAA 
ATTATGGCAT CTGTTTTGAA TGGCAAGTTT GCCGGTCTCA TTAAAGAGTT TGTTATCATT 
GACTGCCGAT ACCCATATGA GTATGAAGGA GGTCACATCA AGGGTGCCGT GAATCTGCAC 
ATGGAAGAAG AGGTTGAAGA CTTCTTACTT AAGAACCCTA TTGTGCCTAC TGATGGCAAG 
CGTGTCATTG TCGTGTTCCA CTGTGAGTTT TCCTCTGAAA GAGGCCCTCG AATGTGCCGA 
TATGTGAGAG AGAGAGATAG GCTCGGCAAT GAGTACCCCA AGCTCCACTA CCCTGAGCTG 
TATGTCCTGA AGGGGGGATA CAAGGAGTTC TTTTTGAAGT GCCAGTCTCA CTGTGAACCC 
CCCAGCTACC GGCCAATGCA CCATGAAGAC TTTAAAGAAG ACCTAAAGAA GTTCCGCACC 
AAGAGCCGGA CCTGGGCAGG AGAAAAGAGC AAAAGAGAGA TGTACAGTCG CCTGAAGAAG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511993.1
CDS	659..2224
Translation	MELGPEPPHRRRLFFACSPTPAPQPTGKMLFGASAAGGLSPVTNLTVTMDQLEGLGSDCEKMEVRNNSSLQRMGSSESTDSGFCLDSPGPLDSKENLEISLTRINSLPKLLGCSPALKRSHSDSLDHDTFHLIDQDENKENEAFEFKKPIRPASRHIYEESKDPFTHRQNSAPARMLSSNESESGNFSPLFIPQSPVKATLSDEDDGFIDLLDGENMKNDEETPSCMASLWTAPLVMRRPANLVGPVSDPKADRCGLFDSPSPCGSSTRAVLKRADRSHEEPPRGTKRRKSVPSPVKAKADVPEPAQLPSQSLSLMSSPKGTIENILDSDPRDLIGDFSKGYLFNTVSGKHQDLKYISPEIMASVLNGKFAGLIKEFVIIDCRYPYEYEGGHIKGAVNLHMEEEVEDFLLKNPIVPTDGKRVIVVFHCEFSSERGPRMCRYVRERDRLGNEYPKLHYPELYVLKGGYKEFFLKCQSHCEPPSYRPMHHEDFKEDLKKFRTKSRTWAGEKSKREMYSRLKKL

Target ORF information:

RefSeq Version	XM_006511930.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cell division cycle 25A (Cdc25a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511930.3

ORF Insert Sequence:

ATGGAACTGG GCCCGGAGCC CCCCCACCGC CGCCGCCTGT TCTTCGCTTG CAGCCCCACG 
CCTGCGCCGC AGCCCACGGG GAAGATGCTG TTTGGCGCGT CAGCTGCTGG CGGACTGTCC 
CCTGTCACCA ACCTGACGGT CACCATGGAC CAGCTGGAAG GACTGGGCAG TGACTGTGAG 
AAAATGGAAG TGAGAAATAA CAGCAGTCTA CAGAGAATGG GCTCCTCAGA ATCGACCGAT 
TCAGGTTTCT GTCTAGATTC TCCTGGGCCC TTGGACAGTA AAGAAAACCT TGAAATTTCC 
CTGACGAGAA TAAATTCCCT ACCTAAGCTC CTGGGATGTA GCCCAGCCCT GAAGAGGAGC 
CACTCTGATT CTCTAGACCA CGACACCTTT CACCTCATCG ACCAGGATGA AAATAAAGAA 
AATGAAGCAT TTGAATTTAA AAAGCCAATA CGACCTGCAT CTCGTCACAT CTACGAGGAG 
AGTAAAGATC CCTTTACACA CAGGCAGAAT TCAGCCCCAG CTCGGATGCT ATCTTCAAAT 
GAAAGTGAAT CAGGAAATTT CAGTCCTCTT TTTATACCCC AGTCACCTGT AAAAGCCACT 
TTGTCCGATG AGGATGATGG CTTCATAGAC CTTCTGGATG GCGAGAATAT GAAGAATGAT 
GAGGAGACCC CATCATGCAT GGCAAGCCTC TGGACAGCTC CCCTTGTCAT GAGGAGACCT 
GCAAACCTTG TTGGACCTGT CTCTGACCCT AAGGCCGATC GTTGCGGGCT GTTTGACTCC 
CCTTCCCCGT GCGGCTCCAG CACTCGGGCG GTGTTGAAGA GAGCAGACCG GTCTCACGAG 
GAGCCTCCTC GGGGTACAAA GAGGAGGAAG AGTGTGCCCA GCCCTGTGAA GGCGAAGGCG 
GATGTTCCGG AGCCCGCCCA GCTTCCATCC CAGTCTCTAT CCCTGATGTC TTCCCCCAAA 
GGAACCATTG AGAACATTTT GGACAGTGAC CCAAGAGACC TTATAGGAGA TTTCTCCAAG 
GGTTATCTCT TTAATACAGT CTCTGGGAAG CATCAGGATT TGAAATATAT TTCTCCAGAA 
ATTATGGCAT CTGTTTTGAA TGGCAAGTTT GCCGGTCTCA TTAAAGAGTT TGTTATCATT 
GACTGCCGAT ACCCATATGA GTATGAAGGA GGTCACATCA AGGGTGCCGT GAATCTGCAC 
ATGGAAGAAG AGGTTGAAGA CTTCTTACTT AAGAACCCTA TTGTGCCTAC TGATGGCAAG 
CGTGTCATTG TCGTGTTCCA CTGTGAGTTT TCCTCTGAAA GAGGCCCTCG AATGTGCCGA 
TATGTGAGAG AGAGAGATAG GCTCGGCAAT GAGTACCCCA AGCTCCACTA CCCTGAGCTG 
TATGTCCTGA AGGGGGGATA CAAGGAGTTC TTTTTGAAGT GCCAGTCTCA CTGTGAACCC 
CCCAGCTACC GGCCAATGCA CCATGAAGAC TTTAAAGAAG ACCTAAAGAA GTTCCGCACC 
AAGAGCCGGA CCTGGGCAGG AGAAAAGAGC AAAAGAGAGA TGTACAGTCG CCTGAAGAAG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc25a ( XM_006511931.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511994.1 Mus musculus cell division cycle 25A (Cdc25a), transcript variant X3, mRNA.

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CloneID	OMu43290
Clone ID Related Accession (Same CDS sequence)	XM_006511931.4 , XM_006511931.3
Accession Version	XM_006511931.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	M-phase inducer phosphatase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006511931.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGG GCCCGGAGCC CCCCCACCGC CGCCGCCTGT TCTTCGCTTG CAGCCCCACG 
CCTGCGCCGC AGCCCACGGG GAAGATGCTG TTTGGCGCGT CAGCTGCTGG CGGACTGTCC 
CCTGTCACCA ACCTGACGGT CACCATGGAC CAGCTGGAAG GACTGGGCAG TGACTGTGAG 
AAAATGGAAG TGAGAAATAA CAGCAGTCTA CAGAGAATGG GCTCCTCAGA ATCGACCGAT 
TCAGGTTTCT GTCTAGATTC TCCTGGGCCC TTGGACAGTA AAGAAAACCT TGAAATTTCC 
CTGACGAGAA TAAATTCCCT ACCTAAGCTC CTGGGATGTA GCCCAGCCCT GAAGAGGAGC 
CACTCTGATT CTCTAGACCA CGACACCTTT CACCTCATCG ACCAGGATGA AAATAAAGAA 
AATGAAGCAT TTGAATTTAA AAAGCCAATA CGACCTGCAT CTCGTCACAT CTACGAGGAG 
AGTAAAGATC CCTTTACACA CAGGCAGAAT TCAGCCCCAG CTCGGATGCT ATCTTCAAAT 
GAAAGTGAAT CAGGAAATTT CAGTCCTCTT TTTATACCCC AGTCACCTGT AAAAGCCACT 
TTGTCCGATG AGGATGATGG CTTCATAGAC CTTCTGGATG GCGAGAATAT GAAGAATGAT 
GAGGAGACCC CATCATGCAT GGCAAGCCTC TGGACAGCTC CCCTTGTCAT GAGGAGACCT 
GCAAACCTTG CCGATCGTTG CGGGCTGTTT GACTCCCCTT CCCCGTGCGG CTCCAGCACT 
CGGGCGGTGT TGAAGAGAGC AGACCGGTCT CACGAGGAGC CTCCTCGGGG TACAAAGAGG 
AGGAAGAGTG TGCCCAGCCC TGTGAAGGCG AAGGCGGATG TTCCGGAGCC CGCCCAGCTT 
CCATCCCAGT CTCTATCCCT GATGTCTTCC CCCAAAGGAA CCATTGAGAA CATTTTGGAC 
AGTGACCCAA GAGACCTTAT AGGAGATTTC TCCAAGGGTT ATCTCTTTAA TACAGTCTCT 
GGGAAGCATC AGGATTTGAA ATATATTTCT CCAGAAATTA TGGCATCTGT TTTGAATGGC 
AAGTTTGCCG GTCTCATTAA AGAGTTTGTT ATCATTGACT GCCGATACCC ATATGAGTAT 
GAAGGAGGTC ACATCAAGGG TGCCGTGAAT CTGCACATGG AAGAAGAGGT TGAAGACTTC 
TTACTTAAGA ACCCTATTGT GCCTACTGAT GGCAAGCGTG TCATTGTCGT GTTCCACTGT 
GAGTTTTCCT CTGAAAGAGG CCCTCGAATG TGCCGATATG TGAGAGAGAG AGATAGGCTC 
GGCAATGAGT ACCCCAAGCT CCACTACCCT GAGCTGTATG TCCTGAAGGG GGGATACAAG 
GAGTTCTTTT TGAAGTGCCA GTCTCACTGT GAACCCCCCA GCTACCGGCC AATGCACCAT 
GAAGACTTTA AAGAAGACCT AAAGAAGTTC CGCACCAAGA GCCGGACCTG GGCAGGAGAA 
AAGAGCAAAA GAGAGATGTA CAGTCGCCTG AAGAAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511994.1
CDS	644..2185
Translation	MELGPEPPHRRRLFFACSPTPAPQPTGKMLFGASAAGGLSPVTNLTVTMDQLEGLGSDCEKMEVRNNSSLQRMGSSESTDSGFCLDSPGPLDSKENLEISLTRINSLPKLLGCSPALKRSHSDSLDHDTFHLIDQDENKENEAFEFKKPIRPASRHIYEESKDPFTHRQNSAPARMLSSNESESGNFSPLFIPQSPVKATLSDEDDGFIDLLDGENMKNDEETPSCMASLWTAPLVMRRPANLADRCGLFDSPSPCGSSTRAVLKRADRSHEEPPRGTKRRKSVPSPVKAKADVPEPAQLPSQSLSLMSSPKGTIENILDSDPRDLIGDFSKGYLFNTVSGKHQDLKYISPEIMASVLNGKFAGLIKEFVIIDCRYPYEYEGGHIKGAVNLHMEEEVEDFLLKNPIVPTDGKRVIVVFHCEFSSERGPRMCRYVRERDRLGNEYPKLHYPELYVLKGGYKEFFLKCQSHCEPPSYRPMHHEDFKEDLKKFRTKSRTWAGEKSKREMYSRLKKL

Target ORF information:

RefSeq Version	XM_006511931.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cell division cycle 25A (Cdc25a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511931.3

ORF Insert Sequence:

ATGGAACTGG GCCCGGAGCC CCCCCACCGC CGCCGCCTGT TCTTCGCTTG CAGCCCCACG 
CCTGCGCCGC AGCCCACGGG GAAGATGCTG TTTGGCGCGT CAGCTGCTGG CGGACTGTCC 
CCTGTCACCA ACCTGACGGT CACCATGGAC CAGCTGGAAG GACTGGGCAG TGACTGTGAG 
AAAATGGAAG TGAGAAATAA CAGCAGTCTA CAGAGAATGG GCTCCTCAGA ATCGACCGAT 
TCAGGTTTCT GTCTAGATTC TCCTGGGCCC TTGGACAGTA AAGAAAACCT TGAAATTTCC 
CTGACGAGAA TAAATTCCCT ACCTAAGCTC CTGGGATGTA GCCCAGCCCT GAAGAGGAGC 
CACTCTGATT CTCTAGACCA CGACACCTTT CACCTCATCG ACCAGGATGA AAATAAAGAA 
AATGAAGCAT TTGAATTTAA AAAGCCAATA CGACCTGCAT CTCGTCACAT CTACGAGGAG 
AGTAAAGATC CCTTTACACA CAGGCAGAAT TCAGCCCCAG CTCGGATGCT ATCTTCAAAT 
GAAAGTGAAT CAGGAAATTT CAGTCCTCTT TTTATACCCC AGTCACCTGT AAAAGCCACT 
TTGTCCGATG AGGATGATGG CTTCATAGAC CTTCTGGATG GCGAGAATAT GAAGAATGAT 
GAGGAGACCC CATCATGCAT GGCAAGCCTC TGGACAGCTC CCCTTGTCAT GAGGAGACCT 
GCAAACCTTG CCGATCGTTG CGGGCTGTTT GACTCCCCTT CCCCGTGCGG CTCCAGCACT 
CGGGCGGTGT TGAAGAGAGC AGACCGGTCT CACGAGGAGC CTCCTCGGGG TACAAAGAGG 
AGGAAGAGTG TGCCCAGCCC TGTGAAGGCG AAGGCGGATG TTCCGGAGCC CGCCCAGCTT 
CCATCCCAGT CTCTATCCCT GATGTCTTCC CCCAAAGGAA CCATTGAGAA CATTTTGGAC 
AGTGACCCAA GAGACCTTAT AGGAGATTTC TCCAAGGGTT ATCTCTTTAA TACAGTCTCT 
GGGAAGCATC AGGATTTGAA ATATATTTCT CCAGAAATTA TGGCATCTGT TTTGAATGGC 
AAGTTTGCCG GTCTCATTAA AGAGTTTGTT ATCATTGACT GCCGATACCC ATATGAGTAT 
GAAGGAGGTC ACATCAAGGG TGCCGTGAAT CTGCACATGG AAGAAGAGGT TGAAGACTTC 
TTACTTAAGA ACCCTATTGT GCCTACTGAT GGCAAGCGTG TCATTGTCGT GTTCCACTGT 
GAGTTTTCCT CTGAAAGAGG CCCTCGAATG TGCCGATATG TGAGAGAGAG AGATAGGCTC 
GGCAATGAGT ACCCCAAGCT CCACTACCCT GAGCTGTATG TCCTGAAGGG GGGATACAAG 
GAGTTCTTTT TGAAGTGCCA GTCTCACTGT GAACCCCCCA GCTACCGGCC AATGCACCAT 
GAAGACTTTA AAGAAGACCT AAAGAAGTTC CGCACCAAGA GCCGGACCTG GGCAGGAGAA 
AAGAGCAAAA GAGAGATGTA CAGTCGCCTG AAGAAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc25a ( XM_006511929.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511992.1 Mus musculus cell division cycle 25A (Cdc25a), transcript variant X1, mRNA.

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CloneID	OMu43288
Clone ID Related Accession (Same CDS sequence)	XM_006511929.3 , XM_006511929.4
Accession Version	XM_006511929.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	M-phase inducer phosphatase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006511929.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGG GCCCGGAGCC CCCCCACCGC CGCCGCCTGT TCTTCGCTTG CAGCCCCACG 
CCTGCGCCGC AGCCCACGGG GAAGATGCTG TTTGGCGCGT CAGCTGCTGG CGGACTGTCC 
CCTGTCACCA ACCTGACGGT CACCATGGAC CAGCTGGAAG GACTGGGCAG TGACTGTGAG 
AAAATGGAAG TGAGAAATAA CAGCAGTCTA CAGAGAATGG GCTCCTCAGA ATCGACCGAT 
TCAGGTTTCT GTCTAGATTC TCCTGGGCCC TTGGACAGTA AAGAAAACCT TGAAATTTCC 
CTGACGAGAA TAAATTCCCT ACCTCAGAAG CTCCTGGGAT GTAGCCCAGC CCTGAAGAGG 
AGCCACTCTG ATTCTCTAGA CCACGACACC TTTCACCTCA TCGACCAGGA TGAAAATAAA 
GAAAATGAAG CATTTGAATT TAAAAAGCCA ATACGACCTG CATCTCGTCA CATCTACGAG 
GAGAGTAAAG ATCCCTTTAC ACACAGGCAG AATTCAGCCC CAGCTCGGAT GCTATCTTCA 
AATGAAAGTG AATCAGGAAA TTTCAGTCCT CTTTTTATAC CCCAGTCACC TGTAAAAGCC 
ACTTTGTCCG ATGAGGATGA TGGCTTCATA GACCTTCTGG ATGGCGAGAA TATGAAGAAT 
GATGAGGAGA CCCCATCATG CATGGCAAGC CTCTGGACAG CTCCCCTTGT CATGAGGAGA 
CCTGCAAACC TTGTTGGACC TGTCTCTGAC CCTAAGGCCG ATCGTTGCGG GCTGTTTGAC 
TCCCCTTCCC CGTGCGGCTC CAGCACTCGG GCGGTGTTGA AGAGAGCAGA CCGGTCTCAC 
GAGGAGCCTC CTCGGGGTAC AAAGAGGAGG AAGAGTGTGC CCAGCCCTGT GAAGGCGAAG 
GCGGATGTTC CGGAGCCCGC CCAGCTTCCA TCCCAGTCTC TATCCCTGAT GTCTTCCCCC 
AAAGGAACCA TTGAGAACAT TTTGGACAGT GACCCAAGAG ACCTTATAGG AGATTTCTCC 
AAGGGTTATC TCTTTAATAC AGTCTCTGGG AAGCATCAGG ATTTGAAATA TATTTCTCCA 
GAAATTATGG CATCTGTTTT GAATGGCAAG TTTGCCGGTC TCATTAAAGA GTTTGTTATC 
ATTGACTGCC GATACCCATA TGAGTATGAA GGAGGTCACA TCAAGGGTGC CGTGAATCTG 
CACATGGAAG AAGAGGTTGA AGACTTCTTA CTTAAGAACC CTATTGTGCC TACTGATGGC 
AAGCGTGTCA TTGTCGTGTT CCACTGTGAG TTTTCCTCTG AAAGAGGCCC TCGAATGTGC 
CGATATGTGA GAGAGAGAGA TAGGCTCGGC AATGAGTACC CCAAGCTCCA CTACCCTGAG 
CTGTATGTCC TGAAGGGGGG ATACAAGGAG TTCTTTTTGA AGTGCCAGTC TCACTGTGAA 
CCCCCCAGCT ACCGGCCAAT GCACCATGAA GACTTTAAAG AAGACCTAAA GAAGTTCCGC 
ACCAAGAGCC GGACCTGGGC AGGAGAAAAG AGCAAAAGAG AGATGTACAG TCGCCTGAAG 
AAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511992.1
CDS	466..2034
Translation	MELGPEPPHRRRLFFACSPTPAPQPTGKMLFGASAAGGLSPVTNLTVTMDQLEGLGSDCEKMEVRNNSSLQRMGSSESTDSGFCLDSPGPLDSKENLEISLTRINSLPQKLLGCSPALKRSHSDSLDHDTFHLIDQDENKENEAFEFKKPIRPASRHIYEESKDPFTHRQNSAPARMLSSNESESGNFSPLFIPQSPVKATLSDEDDGFIDLLDGENMKNDEETPSCMASLWTAPLVMRRPANLVGPVSDPKADRCGLFDSPSPCGSSTRAVLKRADRSHEEPPRGTKRRKSVPSPVKAKADVPEPAQLPSQSLSLMSSPKGTIENILDSDPRDLIGDFSKGYLFNTVSGKHQDLKYISPEIMASVLNGKFAGLIKEFVIIDCRYPYEYEGGHIKGAVNLHMEEEVEDFLLKNPIVPTDGKRVIVVFHCEFSSERGPRMCRYVRERDRLGNEYPKLHYPELYVLKGGYKEFFLKCQSHCEPPSYRPMHHEDFKEDLKKFRTKSRTWAGEKSKREMYSRLKKL

Target ORF information:

RefSeq Version	XM_006511929.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus cell division cycle 25A (Cdc25a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511929.4

ORF Insert Sequence:

ATGGAACTGG GCCCGGAGCC CCCCCACCGC CGCCGCCTGT TCTTCGCTTG CAGCCCCACG 
CCTGCGCCGC AGCCCACGGG GAAGATGCTG TTTGGCGCGT CAGCTGCTGG CGGACTGTCC 
CCTGTCACCA ACCTGACGGT CACCATGGAC CAGCTGGAAG GACTGGGCAG TGACTGTGAG 
AAAATGGAAG TGAGAAATAA CAGCAGTCTA CAGAGAATGG GCTCCTCAGA ATCGACCGAT 
TCAGGTTTCT GTCTAGATTC TCCTGGGCCC TTGGACAGTA AAGAAAACCT TGAAATTTCC 
CTGACGAGAA TAAATTCCCT ACCTCAGAAG CTCCTGGGAT GTAGCCCAGC CCTGAAGAGG 
AGCCACTCTG ATTCTCTAGA CCACGACACC TTTCACCTCA TCGACCAGGA TGAAAATAAA 
GAAAATGAAG CATTTGAATT TAAAAAGCCA ATACGACCTG CATCTCGTCA CATCTACGAG 
GAGAGTAAAG ATCCCTTTAC ACACAGGCAG AATTCAGCCC CAGCTCGGAT GCTATCTTCA 
AATGAAAGTG AATCAGGAAA TTTCAGTCCT CTTTTTATAC CCCAGTCACC TGTAAAAGCC 
ACTTTGTCCG ATGAGGATGA TGGCTTCATA GACCTTCTGG ATGGCGAGAA TATGAAGAAT 
GATGAGGAGA CCCCATCATG CATGGCAAGC CTCTGGACAG CTCCCCTTGT CATGAGGAGA 
CCTGCAAACC TTGTTGGACC TGTCTCTGAC CCTAAGGCCG ATCGTTGCGG GCTGTTTGAC 
TCCCCTTCCC CGTGCGGCTC CAGCACTCGG GCGGTGTTGA AGAGAGCAGA CCGGTCTCAC 
GAGGAGCCTC CTCGGGGTAC AAAGAGGAGG AAGAGTGTGC CCAGCCCTGT GAAGGCGAAG 
GCGGATGTTC CGGAGCCCGC CCAGCTTCCA TCCCAGTCTC TATCCCTGAT GTCTTCCCCC 
AAAGGAACCA TTGAGAACAT TTTGGACAGT GACCCAAGAG ACCTTATAGG AGATTTCTCC 
AAGGGTTATC TCTTTAATAC AGTCTCTGGG AAGCATCAGG ATTTGAAATA TATTTCTCCA 
GAAATTATGG CATCTGTTTT GAATGGCAAG TTTGCCGGTC TCATTAAAGA GTTTGTTATC 
ATTGACTGCC GATACCCATA TGAGTATGAA GGAGGTCACA TCAAGGGTGC CGTGAATCTG 
CACATGGAAG AAGAGGTTGA AGACTTCTTA CTTAAGAACC CTATTGTGCC TACTGATGGC 
AAGCGTGTCA TTGTCGTGTT CCACTGTGAG TTTTCCTCTG AAAGAGGCCC TCGAATGTGC 
CGATATGTGA GAGAGAGAGA TAGGCTCGGC AATGAGTACC CCAAGCTCCA CTACCCTGAG 
CTGTATGTCC TGAAGGGGGG ATACAAGGAG TTCTTTTTGA AGTGCCAGTC TCACTGTGAA 
CCCCCCAGCT ACCGGCCAAT GCACCATGAA GACTTTAAAG AAGACCTAAA GAAGTTCCGC 
ACCAAGAGCC GGACCTGGGC AGGAGAAAAG AGCAAAAGAG AGATGTACAG TCGCCTGAAG 
AAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc25a ( XM_006511930.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511993.1 Mus musculus cell division cycle 25A (Cdc25a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu43289
Clone ID Related Accession (Same CDS sequence)	XM_006511930.3 , XM_006511930.4
Accession Version	XM_006511930.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	M-phase inducer phosphatase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006511930.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGG GCCCGGAGCC CCCCCACCGC CGCCGCCTGT TCTTCGCTTG CAGCCCCACG 
CCTGCGCCGC AGCCCACGGG GAAGATGCTG TTTGGCGCGT CAGCTGCTGG CGGACTGTCC 
CCTGTCACCA ACCTGACGGT CACCATGGAC CAGCTGGAAG GACTGGGCAG TGACTGTGAG 
AAAATGGAAG TGAGAAATAA CAGCAGTCTA CAGAGAATGG GCTCCTCAGA ATCGACCGAT 
TCAGGTTTCT GTCTAGATTC TCCTGGGCCC TTGGACAGTA AAGAAAACCT TGAAATTTCC 
CTGACGAGAA TAAATTCCCT ACCTAAGCTC CTGGGATGTA GCCCAGCCCT GAAGAGGAGC 
CACTCTGATT CTCTAGACCA CGACACCTTT CACCTCATCG ACCAGGATGA AAATAAAGAA 
AATGAAGCAT TTGAATTTAA AAAGCCAATA CGACCTGCAT CTCGTCACAT CTACGAGGAG 
AGTAAAGATC CCTTTACACA CAGGCAGAAT TCAGCCCCAG CTCGGATGCT ATCTTCAAAT 
GAAAGTGAAT CAGGAAATTT CAGTCCTCTT TTTATACCCC AGTCACCTGT AAAAGCCACT 
TTGTCCGATG AGGATGATGG CTTCATAGAC CTTCTGGATG GCGAGAATAT GAAGAATGAT 
GAGGAGACCC CATCATGCAT GGCAAGCCTC TGGACAGCTC CCCTTGTCAT GAGGAGACCT 
GCAAACCTTG TTGGACCTGT CTCTGACCCT AAGGCCGATC GTTGCGGGCT GTTTGACTCC 
CCTTCCCCGT GCGGCTCCAG CACTCGGGCG GTGTTGAAGA GAGCAGACCG GTCTCACGAG 
GAGCCTCCTC GGGGTACAAA GAGGAGGAAG AGTGTGCCCA GCCCTGTGAA GGCGAAGGCG 
GATGTTCCGG AGCCCGCCCA GCTTCCATCC CAGTCTCTAT CCCTGATGTC TTCCCCCAAA 
GGAACCATTG AGAACATTTT GGACAGTGAC CCAAGAGACC TTATAGGAGA TTTCTCCAAG 
GGTTATCTCT TTAATACAGT CTCTGGGAAG CATCAGGATT TGAAATATAT TTCTCCAGAA 
ATTATGGCAT CTGTTTTGAA TGGCAAGTTT GCCGGTCTCA TTAAAGAGTT TGTTATCATT 
GACTGCCGAT ACCCATATGA GTATGAAGGA GGTCACATCA AGGGTGCCGT GAATCTGCAC 
ATGGAAGAAG AGGTTGAAGA CTTCTTACTT AAGAACCCTA TTGTGCCTAC TGATGGCAAG 
CGTGTCATTG TCGTGTTCCA CTGTGAGTTT TCCTCTGAAA GAGGCCCTCG AATGTGCCGA 
TATGTGAGAG AGAGAGATAG GCTCGGCAAT GAGTACCCCA AGCTCCACTA CCCTGAGCTG 
TATGTCCTGA AGGGGGGATA CAAGGAGTTC TTTTTGAAGT GCCAGTCTCA CTGTGAACCC 
CCCAGCTACC GGCCAATGCA CCATGAAGAC TTTAAAGAAG ACCTAAAGAA GTTCCGCACC 
AAGAGCCGGA CCTGGGCAGG AGAAAAGAGC AAAAGAGAGA TGTACAGTCG CCTGAAGAAG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511993.1
CDS	473..2038
Translation	MELGPEPPHRRRLFFACSPTPAPQPTGKMLFGASAAGGLSPVTNLTVTMDQLEGLGSDCEKMEVRNNSSLQRMGSSESTDSGFCLDSPGPLDSKENLEISLTRINSLPKLLGCSPALKRSHSDSLDHDTFHLIDQDENKENEAFEFKKPIRPASRHIYEESKDPFTHRQNSAPARMLSSNESESGNFSPLFIPQSPVKATLSDEDDGFIDLLDGENMKNDEETPSCMASLWTAPLVMRRPANLVGPVSDPKADRCGLFDSPSPCGSSTRAVLKRADRSHEEPPRGTKRRKSVPSPVKAKADVPEPAQLPSQSLSLMSSPKGTIENILDSDPRDLIGDFSKGYLFNTVSGKHQDLKYISPEIMASVLNGKFAGLIKEFVIIDCRYPYEYEGGHIKGAVNLHMEEEVEDFLLKNPIVPTDGKRVIVVFHCEFSSERGPRMCRYVRERDRLGNEYPKLHYPELYVLKGGYKEFFLKCQSHCEPPSYRPMHHEDFKEDLKKFRTKSRTWAGEKSKREMYSRLKKL

Target ORF information:

RefSeq Version	XM_006511930.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus cell division cycle 25A (Cdc25a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511930.4

ORF Insert Sequence:

ATGGAACTGG GCCCGGAGCC CCCCCACCGC CGCCGCCTGT TCTTCGCTTG CAGCCCCACG 
CCTGCGCCGC AGCCCACGGG GAAGATGCTG TTTGGCGCGT CAGCTGCTGG CGGACTGTCC 
CCTGTCACCA ACCTGACGGT CACCATGGAC CAGCTGGAAG GACTGGGCAG TGACTGTGAG 
AAAATGGAAG TGAGAAATAA CAGCAGTCTA CAGAGAATGG GCTCCTCAGA ATCGACCGAT 
TCAGGTTTCT GTCTAGATTC TCCTGGGCCC TTGGACAGTA AAGAAAACCT TGAAATTTCC 
CTGACGAGAA TAAATTCCCT ACCTAAGCTC CTGGGATGTA GCCCAGCCCT GAAGAGGAGC 
CACTCTGATT CTCTAGACCA CGACACCTTT CACCTCATCG ACCAGGATGA AAATAAAGAA 
AATGAAGCAT TTGAATTTAA AAAGCCAATA CGACCTGCAT CTCGTCACAT CTACGAGGAG 
AGTAAAGATC CCTTTACACA CAGGCAGAAT TCAGCCCCAG CTCGGATGCT ATCTTCAAAT 
GAAAGTGAAT CAGGAAATTT CAGTCCTCTT TTTATACCCC AGTCACCTGT AAAAGCCACT 
TTGTCCGATG AGGATGATGG CTTCATAGAC CTTCTGGATG GCGAGAATAT GAAGAATGAT 
GAGGAGACCC CATCATGCAT GGCAAGCCTC TGGACAGCTC CCCTTGTCAT GAGGAGACCT 
GCAAACCTTG TTGGACCTGT CTCTGACCCT AAGGCCGATC GTTGCGGGCT GTTTGACTCC 
CCTTCCCCGT GCGGCTCCAG CACTCGGGCG GTGTTGAAGA GAGCAGACCG GTCTCACGAG 
GAGCCTCCTC GGGGTACAAA GAGGAGGAAG AGTGTGCCCA GCCCTGTGAA GGCGAAGGCG 
GATGTTCCGG AGCCCGCCCA GCTTCCATCC CAGTCTCTAT CCCTGATGTC TTCCCCCAAA 
GGAACCATTG AGAACATTTT GGACAGTGAC CCAAGAGACC TTATAGGAGA TTTCTCCAAG 
GGTTATCTCT TTAATACAGT CTCTGGGAAG CATCAGGATT TGAAATATAT TTCTCCAGAA 
ATTATGGCAT CTGTTTTGAA TGGCAAGTTT GCCGGTCTCA TTAAAGAGTT TGTTATCATT 
GACTGCCGAT ACCCATATGA GTATGAAGGA GGTCACATCA AGGGTGCCGT GAATCTGCAC 
ATGGAAGAAG AGGTTGAAGA CTTCTTACTT AAGAACCCTA TTGTGCCTAC TGATGGCAAG 
CGTGTCATTG TCGTGTTCCA CTGTGAGTTT TCCTCTGAAA GAGGCCCTCG AATGTGCCGA 
TATGTGAGAG AGAGAGATAG GCTCGGCAAT GAGTACCCCA AGCTCCACTA CCCTGAGCTG 
TATGTCCTGA AGGGGGGATA CAAGGAGTTC TTTTTGAAGT GCCAGTCTCA CTGTGAACCC 
CCCAGCTACC GGCCAATGCA CCATGAAGAC TTTAAAGAAG ACCTAAAGAA GTTCCGCACC 
AAGAGCCGGA CCTGGGCAGG AGAAAAGAGC AAAAGAGAGA TGTACAGTCG CCTGAAGAAG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdc25a ( XM_006511931.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511994.1 Mus musculus cell division cycle 25A (Cdc25a), transcript variant X3, mRNA.

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CloneID	OMu43290
Clone ID Related Accession (Same CDS sequence)	XM_006511931.4 , XM_006511931.3
Accession Version	XM_006511931.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	M-phase inducer phosphatase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006511931.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGG GCCCGGAGCC CCCCCACCGC CGCCGCCTGT TCTTCGCTTG CAGCCCCACG 
CCTGCGCCGC AGCCCACGGG GAAGATGCTG TTTGGCGCGT CAGCTGCTGG CGGACTGTCC 
CCTGTCACCA ACCTGACGGT CACCATGGAC CAGCTGGAAG GACTGGGCAG TGACTGTGAG 
AAAATGGAAG TGAGAAATAA CAGCAGTCTA CAGAGAATGG GCTCCTCAGA ATCGACCGAT 
TCAGGTTTCT GTCTAGATTC TCCTGGGCCC TTGGACAGTA AAGAAAACCT TGAAATTTCC 
CTGACGAGAA TAAATTCCCT ACCTAAGCTC CTGGGATGTA GCCCAGCCCT GAAGAGGAGC 
CACTCTGATT CTCTAGACCA CGACACCTTT CACCTCATCG ACCAGGATGA AAATAAAGAA 
AATGAAGCAT TTGAATTTAA AAAGCCAATA CGACCTGCAT CTCGTCACAT CTACGAGGAG 
AGTAAAGATC CCTTTACACA CAGGCAGAAT TCAGCCCCAG CTCGGATGCT ATCTTCAAAT 
GAAAGTGAAT CAGGAAATTT CAGTCCTCTT TTTATACCCC AGTCACCTGT AAAAGCCACT 
TTGTCCGATG AGGATGATGG CTTCATAGAC CTTCTGGATG GCGAGAATAT GAAGAATGAT 
GAGGAGACCC CATCATGCAT GGCAAGCCTC TGGACAGCTC CCCTTGTCAT GAGGAGACCT 
GCAAACCTTG CCGATCGTTG CGGGCTGTTT GACTCCCCTT CCCCGTGCGG CTCCAGCACT 
CGGGCGGTGT TGAAGAGAGC AGACCGGTCT CACGAGGAGC CTCCTCGGGG TACAAAGAGG 
AGGAAGAGTG TGCCCAGCCC TGTGAAGGCG AAGGCGGATG TTCCGGAGCC CGCCCAGCTT 
CCATCCCAGT CTCTATCCCT GATGTCTTCC CCCAAAGGAA CCATTGAGAA CATTTTGGAC 
AGTGACCCAA GAGACCTTAT AGGAGATTTC TCCAAGGGTT ATCTCTTTAA TACAGTCTCT 
GGGAAGCATC AGGATTTGAA ATATATTTCT CCAGAAATTA TGGCATCTGT TTTGAATGGC 
AAGTTTGCCG GTCTCATTAA AGAGTTTGTT ATCATTGACT GCCGATACCC ATATGAGTAT 
GAAGGAGGTC ACATCAAGGG TGCCGTGAAT CTGCACATGG AAGAAGAGGT TGAAGACTTC 
TTACTTAAGA ACCCTATTGT GCCTACTGAT GGCAAGCGTG TCATTGTCGT GTTCCACTGT 
GAGTTTTCCT CTGAAAGAGG CCCTCGAATG TGCCGATATG TGAGAGAGAG AGATAGGCTC 
GGCAATGAGT ACCCCAAGCT CCACTACCCT GAGCTGTATG TCCTGAAGGG GGGATACAAG 
GAGTTCTTTT TGAAGTGCCA GTCTCACTGT GAACCCCCCA GCTACCGGCC AATGCACCAT 
GAAGACTTTA AAGAAGACCT AAAGAAGTTC CGCACCAAGA GCCGGACCTG GGCAGGAGAA 
AAGAGCAAAA GAGAGATGTA CAGTCGCCTG AAGAAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511994.1
CDS	473..2014
Translation	MELGPEPPHRRRLFFACSPTPAPQPTGKMLFGASAAGGLSPVTNLTVTMDQLEGLGSDCEKMEVRNNSSLQRMGSSESTDSGFCLDSPGPLDSKENLEISLTRINSLPKLLGCSPALKRSHSDSLDHDTFHLIDQDENKENEAFEFKKPIRPASRHIYEESKDPFTHRQNSAPARMLSSNESESGNFSPLFIPQSPVKATLSDEDDGFIDLLDGENMKNDEETPSCMASLWTAPLVMRRPANLADRCGLFDSPSPCGSSTRAVLKRADRSHEEPPRGTKRRKSVPSPVKAKADVPEPAQLPSQSLSLMSSPKGTIENILDSDPRDLIGDFSKGYLFNTVSGKHQDLKYISPEIMASVLNGKFAGLIKEFVIIDCRYPYEYEGGHIKGAVNLHMEEEVEDFLLKNPIVPTDGKRVIVVFHCEFSSERGPRMCRYVRERDRLGNEYPKLHYPELYVLKGGYKEFFLKCQSHCEPPSYRPMHHEDFKEDLKKFRTKSRTWAGEKSKREMYSRLKKL

Target ORF information:

RefSeq Version	XM_006511931.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus cell division cycle 25A (Cdc25a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511931.4

ORF Insert Sequence:

ATGGAACTGG GCCCGGAGCC CCCCCACCGC CGCCGCCTGT TCTTCGCTTG CAGCCCCACG 
CCTGCGCCGC AGCCCACGGG GAAGATGCTG TTTGGCGCGT CAGCTGCTGG CGGACTGTCC 
CCTGTCACCA ACCTGACGGT CACCATGGAC CAGCTGGAAG GACTGGGCAG TGACTGTGAG 
AAAATGGAAG TGAGAAATAA CAGCAGTCTA CAGAGAATGG GCTCCTCAGA ATCGACCGAT 
TCAGGTTTCT GTCTAGATTC TCCTGGGCCC TTGGACAGTA AAGAAAACCT TGAAATTTCC 
CTGACGAGAA TAAATTCCCT ACCTAAGCTC CTGGGATGTA GCCCAGCCCT GAAGAGGAGC 
CACTCTGATT CTCTAGACCA CGACACCTTT CACCTCATCG ACCAGGATGA AAATAAAGAA 
AATGAAGCAT TTGAATTTAA AAAGCCAATA CGACCTGCAT CTCGTCACAT CTACGAGGAG 
AGTAAAGATC CCTTTACACA CAGGCAGAAT TCAGCCCCAG CTCGGATGCT ATCTTCAAAT 
GAAAGTGAAT CAGGAAATTT CAGTCCTCTT TTTATACCCC AGTCACCTGT AAAAGCCACT 
TTGTCCGATG AGGATGATGG CTTCATAGAC CTTCTGGATG GCGAGAATAT GAAGAATGAT 
GAGGAGACCC CATCATGCAT GGCAAGCCTC TGGACAGCTC CCCTTGTCAT GAGGAGACCT 
GCAAACCTTG CCGATCGTTG CGGGCTGTTT GACTCCCCTT CCCCGTGCGG CTCCAGCACT 
CGGGCGGTGT TGAAGAGAGC AGACCGGTCT CACGAGGAGC CTCCTCGGGG TACAAAGAGG 
AGGAAGAGTG TGCCCAGCCC TGTGAAGGCG AAGGCGGATG TTCCGGAGCC CGCCCAGCTT 
CCATCCCAGT CTCTATCCCT GATGTCTTCC CCCAAAGGAA CCATTGAGAA CATTTTGGAC 
AGTGACCCAA GAGACCTTAT AGGAGATTTC TCCAAGGGTT ATCTCTTTAA TACAGTCTCT 
GGGAAGCATC AGGATTTGAA ATATATTTCT CCAGAAATTA TGGCATCTGT TTTGAATGGC 
AAGTTTGCCG GTCTCATTAA AGAGTTTGTT ATCATTGACT GCCGATACCC ATATGAGTAT 
GAAGGAGGTC ACATCAAGGG TGCCGTGAAT CTGCACATGG AAGAAGAGGT TGAAGACTTC 
TTACTTAAGA ACCCTATTGT GCCTACTGAT GGCAAGCGTG TCATTGTCGT GTTCCACTGT 
GAGTTTTCCT CTGAAAGAGG CCCTCGAATG TGCCGATATG TGAGAGAGAG AGATAGGCTC 
GGCAATGAGT ACCCCAAGCT CCACTACCCT GAGCTGTATG TCCTGAAGGG GGGATACAAG 
GAGTTCTTTT TGAAGTGCCA GTCTCACTGT GAACCCCCCA GCTACCGGCC AATGCACCAT 
GAAGACTTTA AAGAAGACCT AAAGAAGTTC CGCACCAAGA GCCGGACCTG GGCAGGAGAA 
AAGAGCAAAA GAGAGATGTA CAGTCGCCTG AAGAAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cell cycle-targeting microRNAs promote differentiation by enforcing cell-cycle exit.
Proceedings of the National Academy of Sciences of the United States of America114(40)10660-10665(2017 Oct)
Otto T,Candido SV,Pilarz MS,Sicinska E,Bronson RT,Bowden M,Lachowicz IA,Mulry K,Fassl A,Han RC,Jecrois ES,Sicinski P

Cdc25A Is a Critical Mediator of Ischemic Neuronal Death
The Journal of neuroscience : the official journal of the Society for Neuroscience37(28)6729-6740(2017 Jul)
Iyirhiaro GO,Im DS,Boonying W,Callaghan SM,During MJ,Slack RS,Park DS

A Genome-wide CRISPR Screen Identifies CDC25A as a Determinant of Sensitivity to ATR Inhibitors.
Molecular cell62(2)307-13(2016 04)
Ruiz S,Mayor-Ruiz C,Lafarga V,Murga M,Vega-Sendino M,Ortega S,Fernandez-Capetillo O

Oocyte-expressed yes-associated protein is a key activator of the early zygotic genome in mouse.
Cell research26(3)275-87(2016 Mar)
Yu C,Ji SY,Dang YJ,Sha QQ,Yuan YF,Zhou JJ,Yan LY,Qiao J,Tang F,Fan HY

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Cdc25a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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