Ccnf cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ccnf
Entrez Gene ID	12449
Full Name	cyclin F
Synonyms	CycF,Fbxo1
General protein information	Preferred Names cyclin F Names cyclin-F G2/mitotic-specific cyclin-F
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 A3.3\|17 12.32 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_007634.4	NP_031660.3	cyclin-F
XM_006523554.1	XP_006523617.1	cyclin-F isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ccnf	NP_001093944.1
Xenopus tropicalis (tropical clawed frog)	ccnf	NP_001011083.1
Homo sapiens (human)	CCNF	NP_001752.2
Mus musculus (house mouse)	Ccnf	NP_031660.3
Danio rerio (zebrafish)	ccnf	NP_996931.1
Bos taurus (cattle)	CCNF	NP_001092340.1
	CCNF	XP_001085466.1
Pan troglodytes (chimpanzee)	CCNF	XP_510746.2
Canis lupus familiaris (dog)	CCNF	XP_005621742.1
Gallus gallus (chicken)	CCNF	XP_415043.4

Gene Ontology
Bibliography

Related articles in PubMed

The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells.
Ray JP, Staron MM, Shyer JA, Ho PC, Marshall HD, Gray SM, Laidlaw BJ, Araki K, Ahmed R, Kaech SM, Craft J
Immunity43(4)690-702(2015 Oct)

Congenital asplenia in mice and humans with mutations in a Pbx/Nkx2-5/p15 module.
Koss M, Bolze A, Brendolan A, Saggese M, Capellini TD, Bojilova E, Boisson B, Prall OW, Elliott DA, Solloway M, Lenti E, Hidaka C, Chang CP, Mahlaoui N, Harvey RP, Casanova JL, Selleri L
Developmental cell22(5)913-26(2012 May)

The protein kinase KIS impacts gene expression during development and fear conditioning in adult mice.
Manceau V, Kremmer E, Nabel EG, Maucuer A
PloS one7(8)e43946(2012)

SCF(Cyclin F) controls centrosome homeostasis and mitotic fidelity through CP110 degradation.
D'Angiolella V, Donato V, Vijayakumar S, Saraf A, Florens L, Washburn MP, Dynlacht B, Pagano M
Nature466(7302)138-42(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SCF(Cyclin F)-mediated degradation of CP110 is required for the fidelity of mitosis and genome integrity
Title: SCF(Cyclin F) controls centrosome homeostasis and mitotic fidelity through CP110 degradation.

CycF(-/-) animals, with a myriad of developmental anomalies due in large part to failures in yolk sac and chorioallantoic placentation, die around embryonic day 10.5.
Title: Cyclin F disruption compromises placental development and affects normal cell cycle execution.

The following Ccnf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ccnf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00511	NM_007634.4 Latest version!	Mus musculus cyclin F (Ccnf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$454.30 $649.00
OMu53061	XM_006523554.1 Latest version!	Mus musculus cyclin F (Ccnf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ccnf ( NM_007634.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031660.3 Mus musculus cyclin F (Ccnf), mRNA.

Price & Availability↑

CloneID	OMu00511
Clone ID Related Accession (Same CDS sequence)	NM_007634.4
Accession Version	NM_007634.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	cyclin-F
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK159594.1. On Jun 3, 2007 this sequence version replaced NM_007634.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK159594.1, AK030298.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGGGAGCG GCGGTGTGAT CCATTGTAGG TGTGCCAAGT GTTTCTGTTA TCCTACTAAA 
CGAAGAATCA AAAGAAGACC CAGAAACTTA ACCATCTTGA GTCTCCCAGA AGATGTACTC 
TTTCATATCC TGAAATGGCT TTCTGTTGGG GACATCCTTG CTGTCCGAGC TGTCCACTCC 
CACCTCAAGT ACCTGGTGGA CAACCATGCC AGTGTGTGGG CATCTGCCAG CTTCCAGGAG 
CTGTGGCCTT CTCCACAGAA CCTGAAGCTC TTTGAAAGGG CTGCTGAAAA GGGAAATTTT 
GAAGCTGCTG TGAAGTTGGG GATTGCCTAC CTCTACAATG AAGGCCTGTC TGTGTCAGAT 
GAGGCCTGCG CAGAAGTGAA CGGCTTGAAG GCCTCTCGCT TCTTCAGCAT GGCTGAGAGA 
CTGAATACGG GTTCTGAGCC CTTCATCTGG CTCTTCATCC GCCCACCGTG GTCAGTGTCC 
GGAAGCTGCT GCAAGGCTGT GGTTCATGAC AGCCTCCGAG CGGAGTGTCA GTTACAGAGA 
AGTCATAAAG CTTCCATACT ACACTGCTTG GGAAGGGTGC TAAATCTTTT TGAGGACGAA 
GAGAAAAGGA AGCAGGCGCG TAGCCTTTTG GAAGAGTCTT CTCGTCAGGG ATGCTTAATC 
AGCTCATACC TCCTTTGGGA AAGCGACAGA AAGGTGGATA TGTCCGACCC CGGACGATGC 
CTCCACAGCT TCCGGAAACT CAGGGACTAC GCTGCGAAAG GCTGCTGGGA AGCACAGCTG 
GCTTTGGCCA AAGCCTGTGC AGGTGGAAGC CAACTTGGAC TGGAAGGGAA GGCCTGCAGT 
GAGTCGGTCT GCCAGCTCTT CCAGGCCTCC CAGGCTGTCA ACAAGCAGCA GATATTCTCT 
GTGCAGAAGG GGCTCAGCGA CACCATGAGG TACATTCTAA TCGACTGGTT GGTGGAAGTT 
GCCACAATGA AGGACTTTAC AAGCCTGTGT CTTCACCTGA CTGTGGAGTG TGTAGACCGG 
TACCTGCGAA GGAGGCTGGT CCCCAGATAC AAGCTGCAGC TCCTGGGTAT TGCCTGCATG 
GTCATCTGTA CCCGGTTCAT CAGCAAAGAG ATTCTGACGA TTAGAGAGGC TGTGTGGCTC 
ACAGACAACA CGTATAAATA CGAGGACTTG GTGCGCGTGA TGGGGGAGAT CATTTCCGCC 
CTGGAAGGGA AGATTCGGAT CCCTACCGTG GTTGACTATA AAGAGGTCCT GCTGACACTG 
GTCCCCGTCG CACCCAGAAC CCAGCACCTG TGCAGCTTCC TCTGTGAGCT CACCCTGCTG 
CACACCAGCC TGTCCATCTA TGCCCCAGCC CGCCTGGCCT CTGCGGCCCT TCTCCTGGCC 
AGACTCATGC ATGGGCAGAC ACAGCCCTGG ACCACTCATC TGTGGGACCT CACTGGCTTC 
TCCTACAGTG ACCTCGTACC CTGTGTCCTG AGCCTTCATA AGAAGTGTTT CCACGATGAT 
GCACCCAAAG ACTACAGACA AGTCTCTCTG ACGGCCGTGA AGCAGAGGTT CGAGGATAAG 
TGCTATGAGG AAATCAGCCG GGAAGAGGTG CTGAGCTATG CTGACTTGTG CAGTACAATA 
GGAGTAAAAC AGGAAAGCCC AGAGCCCCCA TCTTTCCCTA GCTCAGGGGA GATCCACACC 
TTCCTCAGCT CGCCCTCCGG GAGGAGGAGC AAACGGAAGC GAGAAAACAG CCTTCAGGAG 
GACAGAGGCA GCTTTGTCAC CACACCCACC GCAGAGCTAT CGAATCAGGA GGAGACACTG 
CTAGGCAGCC TTCTGGACTG GAGCCTGGAG TGCTGCTCTG GCTATGAGGG AGACCAGGAG 
AGCGAAGGCG AGAAGGAGGG TGATGTCACA GCTCCCAGCA GACTCCTTGA TGTCACTGTG 
GTCTACCTGA ACCCAGAAGA ACATTGCTGC CAGGAGTCCA GTGATGAGGA GGCCTGGCCA 
GAGGACAAGA TCCACCCAGC ACCAGGCACC CAGGCACCTC CAGCCTCAGC TCCCAGGCCC 
CTTCTCTGCA ATCGGGGAGA TCGGGCCAAG GACATTACGA CCTCAGGATA CTCCTCTGTT 
AGCAGCTCGA GTCCCATAAG CTCCCTGGAT GGTGGCATGG GGGGCTCTCC GCAATCTACC 
TCAGTGCTCT CTGTGGGCAG CCACTCAAGC ACAAAGCCTT GCCACCATCA GGCCAAGAAG 
TCATGTTTAC AGTGTCGTCC CCCAAACTCC CCAGAGAGCG GTGTTCACCA GCAGCCTGTA 
AAGCGCCAAA ACCTGTCTGT ACACAGTGAC AAGGACATGC ACCTGGCTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031660.3
CDS	100..2433
Translation	MGSGGVIHCRCAKCFCYPTKRRIKRRPRNLTILSLPEDVLFHILKWLSVGDILAVRAVHSHLKYLVDNHASVWASASFQELWPSPQNLKLFERAAEKGNFEAAVKLGIAYLYNEGLSVSDEACAEVNGLKASRFFSMAERLNTGSEPFIWLFIRPPWSVSGSCCKAVVHDSLRAECQLQRSHKASILHCLGRVLNLFEDEEKRKQARSLLEESSRQGCLISSYLLWESDRKVDMSDPGRCLHSFRKLRDYAAKGCWEAQLALAKACAGGSQLGLEGKACSESVCQLFQASQAVNKQQIFSVQKGLSDTMRYILIDWLVEVATMKDFTSLCLHLTVECVDRYLRRRLVPRYKLQLLGIACMVICTRFISKEILTIREAVWLTDNTYKYEDLVRVMGEIISALEGKIRIPTVVDYKEVLLTLVPVAPRTQHLCSFLCELTLLHTSLSIYAPARLASAALLLARLMHGQTQPWTTHLWDLTGFSYSDLVPCVLSLHKKCFHDDAPKDYRQVSLTAVKQRFEDKCYEEISREEVLSYADLCSTIGVKQESPEPPSFPSSGEIHTFLSSPSGRRSKRKRENSLQEDRGSFVTTPTAELSNQEETLLGSLLDWSLECCSGYEGDQESEGEKEGDVTAPSRLLDVTVVYLNPEEHCCQESSDEEAWPEDKIHPAPGTQAPPASAPRPLLCNRGDRAKDITTSGYSSVSSSSPISSLDGGMGGSPQSTSVLSVGSHSSTKPCHHQAKKSCLQCRPPNSPESGVHQQPVKRQNLSVHSDKDMHLAS

Target ORF information:

RefSeq Version	NM_007634.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus cyclin F (Ccnf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007634.4

ORF Insert Sequence:

ATGGGGAGCG GCGGTGTGAT CCATTGTAGG TGTGCCAAGT GTTTCTGTTA TCCTACTAAA 
CGAAGAATCA AAAGAAGACC CAGAAACTTA ACCATCTTGA GTCTCCCAGA AGATGTACTC 
TTTCATATCC TGAAATGGCT TTCTGTTGGG GACATCCTTG CTGTCCGAGC TGTCCACTCC 
CACCTCAAGT ACCTGGTGGA CAACCATGCC AGTGTGTGGG CATCTGCCAG CTTCCAGGAG 
CTGTGGCCTT CTCCACAGAA CCTGAAGCTC TTTGAAAGGG CTGCTGAAAA GGGAAATTTT 
GAAGCTGCTG TGAAGTTGGG GATTGCCTAC CTCTACAATG AAGGCCTGTC TGTGTCAGAT 
GAGGCCTGCG CAGAAGTGAA CGGCTTGAAG GCCTCTCGCT TCTTCAGCAT GGCTGAGAGA 
CTGAATACGG GTTCTGAGCC CTTCATCTGG CTCTTCATCC GCCCACCGTG GTCAGTGTCC 
GGAAGCTGCT GCAAGGCTGT GGTTCATGAC AGCCTCCGAG CGGAGTGTCA GTTACAGAGA 
AGTCATAAAG CTTCCATACT ACACTGCTTG GGAAGGGTGC TAAATCTTTT TGAGGACGAA 
GAGAAAAGGA AGCAGGCGCG TAGCCTTTTG GAAGAGTCTT CTCGTCAGGG ATGCTTAATC 
AGCTCATACC TCCTTTGGGA AAGCGACAGA AAGGTGGATA TGTCCGACCC CGGACGATGC 
CTCCACAGCT TCCGGAAACT CAGGGACTAC GCTGCGAAAG GCTGCTGGGA AGCACAGCTG 
GCTTTGGCCA AAGCCTGTGC AGGTGGAAGC CAACTTGGAC TGGAAGGGAA GGCCTGCAGT 
GAGTCGGTCT GCCAGCTCTT CCAGGCCTCC CAGGCTGTCA ACAAGCAGCA GATATTCTCT 
GTGCAGAAGG GGCTCAGCGA CACCATGAGG TACATTCTAA TCGACTGGTT GGTGGAAGTT 
GCCACAATGA AGGACTTTAC AAGCCTGTGT CTTCACCTGA CTGTGGAGTG TGTAGACCGG 
TACCTGCGAA GGAGGCTGGT CCCCAGATAC AAGCTGCAGC TCCTGGGTAT TGCCTGCATG 
GTCATCTGTA CCCGGTTCAT CAGCAAAGAG ATTCTGACGA TTAGAGAGGC TGTGTGGCTC 
ACAGACAACA CGTATAAATA CGAGGACTTG GTGCGCGTGA TGGGGGAGAT CATTTCCGCC 
CTGGAAGGGA AGATTCGGAT CCCTACCGTG GTTGACTATA AAGAGGTCCT GCTGACACTG 
GTCCCCGTCG CACCCAGAAC CCAGCACCTG TGCAGCTTCC TCTGTGAGCT CACCCTGCTG 
CACACCAGCC TGTCCATCTA TGCCCCAGCC CGCCTGGCCT CTGCGGCCCT TCTCCTGGCC 
AGACTCATGC ATGGGCAGAC ACAGCCCTGG ACCACTCATC TGTGGGACCT CACTGGCTTC 
TCCTACAGTG ACCTCGTACC CTGTGTCCTG AGCCTTCATA AGAAGTGTTT CCACGATGAT 
GCACCCAAAG ACTACAGACA AGTCTCTCTG ACGGCCGTGA AGCAGAGGTT CGAGGATAAG 
TGCTATGAGG AAATCAGCCG GGAAGAGGTG CTGAGCTATG CTGACTTGTG CAGTACAATA 
GGAGTAAAAC AGGAAAGCCC AGAGCCCCCA TCTTTCCCTA GCTCAGGGGA GATCCACACC 
TTCCTCAGCT CGCCCTCCGG GAGGAGGAGC AAACGGAAGC GAGAAAACAG CCTTCAGGAG 
GACAGAGGCA GCTTTGTCAC CACACCCACC GCAGAGCTAT CGAATCAGGA GGAGACACTG 
CTAGGCAGCC TTCTGGACTG GAGCCTGGAG TGCTGCTCTG GCTATGAGGG AGACCAGGAG 
AGCGAAGGCG AGAAGGAGGG TGATGTCACA GCTCCCAGCA GACTCCTTGA TGTCACTGTG 
GTCTACCTGA ACCCAGAAGA ACATTGCTGC CAGGAGTCCA GTGATGAGGA GGCCTGGCCA 
GAGGACAAGA TCCACCCAGC ACCAGGCACC CAGGCACCTC CAGCCTCAGC TCCCAGGCCC 
CTTCTCTGCA ATCGGGGAGA TCGGGCCAAG GACATTACGA CCTCAGGATA CTCCTCTGTT 
AGCAGCTCGA GTCCCATAAG CTCCCTGGAT GGTGGCATGG GGGGCTCTCC GCAATCTACC 
TCAGTGCTCT CTGTGGGCAG CCACTCAAGC ACAAAGCCTT GCCACCATCA GGCCAAGAAG 
TCATGTTTAC AGTGTCGTCC CCCAAACTCC CCAGAGAGCG GTGTTCACCA GCAGCCTGTA 
AAGCGCCAAA ACCTGTCTGT ACACAGTGAC AAGGACATGC ACCTGGCTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ccnf ( XM_006523554.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523617.1 Mus musculus cyclin F (Ccnf), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53061
Clone ID Related Accession (Same CDS sequence)	XM_006523554.1
Accession Version	XM_006523554.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cyclin-F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCGG GCCCCTGTGA CCCGGGAGTG CGCGTCGCAT CCCCCAAAGC ACGGGTGATC 
CATTGTAGGT GTGCCAAGTG TTTCTGTTAT CCTACTAAAC GAAGAATCAA AAGAAGACCC 
AGAAACTTAA CCATCTTGAG TCTCCCAGAA GATGTACTCT TTCATATCCT GAAATGGCTT 
TCTGTTGGGG ACATCCTTGC TGTCCGAGCT GTCCACTCCC ACCTCAAGTA CCTGGTGGAC 
AACCATGCCA GTGTGTGGGC ATCTGCCAGC TTCCAGGAGC TGTGGCCTTC TCCACAGAAC 
CTGAAGCTCT TTGAAAGGGC TGCTGAAAAG GGAAATTTTG AAGCTGCTGT GAAGTTGGGG 
ATTGCCTACC TCTACAATGA AGGCCTGTCT GTGTCAGATG AGGCCTGCGC AGAAGTGAAC 
GGCTTGAAGG CCTCTCGCTT CTTCAGCATG GCTGAGAGAC TGAATACGGG TTCTGAGCCC 
TTCATCTGGC TCTTCATCCG CCCACCGTGG TCAGTGTCCG GAAGCTGCTG CAAGGCTGTG 
GTTCATGACA GCCTCCGAGC GGAGTGTCAG TTACAGAGAA GTCATAAAGC TTCCATACTA 
CACTGCTTGG GAAGGGTGCT AAATCTTTTT GAGGACGAAG AGAAAAGGAA GCAGGCGCGT 
AGCCTTTTGG AAGAGTCTTC TCGTCAGGGA TGCTTAATCA GCTCATACCT CCTTTGGGAA 
AGCGACAGAA AGGTGGATAT GTCCGACCCC GGACGATGCC TCCACAGCTT CCGGAAACTC 
AGGGACTACG CTGCGAAAGG CTGCTGGGAA GCACAGCTGG CTTTGGCCAA AGCCTGTGCA 
GGTGGAAGCC AACTTGGACT GGAAGGGAAG GCCTGCAGTG AGTCGGTCTG CCAGCTCTTC 
CAGGCCTCCC AGGCTGTCAA CAAGCAGCAG ATATTCTCTG TGCAGAAGGG GCTCAGCGAC 
ACCATGAGGT ACATTCTAAT CGACTGGTTG GTGGAAGTTG CCACAATGAA GGACTTTACA 
AGCCTGTGTC TTCACCTGAC TGTGGAGTGT GTAGACCGGT ACCTGCGAAG GAGGCTGGTC 
CCCAGATACA AGCTGCAGCT CCTGGGTATT GCCTGCATGG TCATCTGTAC CCGGTTCATC 
AGCAAAGAGA TTCTGACGAT TAGAGAGGCT GTGTGGCTCA CAGACAACAC GTATAAATAC 
GAGGACTTGG TGCGCGTGAT GGGGGAGATC ATTTCCGCCC TGGAAGGGAA GATTCGGATC 
CCTACCGTGG TTGACTATAA AGAGGTCCTG CTGACACTGG TCCCCGTCGC ACCCAGAACC 
CAGCACCTGT GCAGCTTCCT CTGTGAGCTC ACCCTGCTGC ACACCAGCCT GTCCATCTAT 
GCCCCAGCCC GCCTGGCCTC TGCGGCCCTT CTCCTGGCCA GACTCATGCA TGGGCAGACA 
CAGCCCTGGA CCACTCATCT GTGGGACCTC ACTGGCTTCT CCTACAGTGA CCTCGTACCC 
TGTGTCCTGA GCCTTCATAA GAAGTGTTTC CACGATGATG CACCCAAAGA CTACAGACAA 
GTCTCTCTGA CGGCCGTGAA GCAGAGGTTC GAGGATAAGT GCTATGAGGA AATCAGCCGG 
GAAGAGGTGC TGAGCTATGC TGACTTGTGC AGTACAATAG GAGTAAAACA GGAAAGCCCA 
GAGCCCCCAT CTTTCCCTAG CTCAGGGGAG ATCCACACCT TCCTCAGCTC GCCCTCCGGG 
AGGAGGAGCA AACGGAAGCG AGAAAACAGC CTTCAGGAGG ACAGAGGCAG CTTTGTCACC 
ACACCCACCG CAGAGCTATC GAATCAGGAG GAGACACTGC TAGGCAGCCT TCTGGACTGG 
AGCCTGGAGT GCTGCTCTGG CTATGAGGGA GACCAGGAGA GCGAAGGCGA GAAGGAGGGT 
GATGTCACAG CTCCCAGCAG ACTCCTTGAT GTCACTGTGG TCTACCTGAA CCCAGAAGAA 
CATTGCTGCC AGGAGTCCAG TGATGAGGAG GCCTGGCCAG AGGACAAGAT CCACCCAGCA 
CCAGGCACCC AGGCACCTCC AGCCTCAGCT CCCAGGCCCC TTCTCTGCAA TCGGGGAGAT 
CGGGCCAAGG ACATTACGAC CTCAGGATAC TCCTCTGTTA GCAGCTCGAG TCCCATAAGC 
TCCCTGGATG GTGGCATGGG GGGCTCTCCG CAATCTACCT CAGTGCTCTC TGTGGGCAGC 
CACTCAAGCA CAAAGCCTTG CCACCATCAG GCCAAGAAGT CATGTTTACA GTGTCGTCCC 
CCAAACTCCC CAGAGAGCGG TGTTCACCAG CAGCCTGTAA AGCGCCAAAA CCTGTCTGTA 
CACAGTGACA AGGACATGCA CCTGGCTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523617.1
CDS	108..2480
Translation	MDSGPCDPGVRVASPKARVIHCRCAKCFCYPTKRRIKRRPRNLTILSLPEDVLFHILKWLSVGDILAVRAVHSHLKYLVDNHASVWASASFQELWPSPQNLKLFERAAEKGNFEAAVKLGIAYLYNEGLSVSDEACAEVNGLKASRFFSMAERLNTGSEPFIWLFIRPPWSVSGSCCKAVVHDSLRAECQLQRSHKASILHCLGRVLNLFEDEEKRKQARSLLEESSRQGCLISSYLLWESDRKVDMSDPGRCLHSFRKLRDYAAKGCWEAQLALAKACAGGSQLGLEGKACSESVCQLFQASQAVNKQQIFSVQKGLSDTMRYILIDWLVEVATMKDFTSLCLHLTVECVDRYLRRRLVPRYKLQLLGIACMVICTRFISKEILTIREAVWLTDNTYKYEDLVRVMGEIISALEGKIRIPTVVDYKEVLLTLVPVAPRTQHLCSFLCELTLLHTSLSIYAPARLASAALLLARLMHGQTQPWTTHLWDLTGFSYSDLVPCVLSLHKKCFHDDAPKDYRQVSLTAVKQRFEDKCYEEISREEVLSYADLCSTIGVKQESPEPPSFPSSGEIHTFLSSPSGRRSKRKRENSLQEDRGSFVTTPTAELSNQEETLLGSLLDWSLECCSGYEGDQESEGEKEGDVTAPSRLLDVTVVYLNPEEHCCQESSDEEAWPEDKIHPAPGTQAPPASAPRPLLCNRGDRAKDITTSGYSSVSSSSPISSLDGGMGGSPQSTSVLSVGSHSSTKPCHHQAKKSCLQCRPPNSPESGVHQQPVKRQNLSVHSDKDMHLAS

Target ORF information:

RefSeq Version	XM_006523554.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cyclin F (Ccnf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523554.1

ORF Insert Sequence:

ATGGACTCGG GCCCCTGTGA CCCGGGAGTG CGCGTCGCAT CCCCCAAAGC ACGGGTGATC 
CATTGTAGGT GTGCCAAGTG TTTCTGTTAT CCTACTAAAC GAAGAATCAA AAGAAGACCC 
AGAAACTTAA CCATCTTGAG TCTCCCAGAA GATGTACTCT TTCATATCCT GAAATGGCTT 
TCTGTTGGGG ACATCCTTGC TGTCCGAGCT GTCCACTCCC ACCTCAAGTA CCTGGTGGAC 
AACCATGCCA GTGTGTGGGC ATCTGCCAGC TTCCAGGAGC TGTGGCCTTC TCCACAGAAC 
CTGAAGCTCT TTGAAAGGGC TGCTGAAAAG GGAAATTTTG AAGCTGCTGT GAAGTTGGGG 
ATTGCCTACC TCTACAATGA AGGCCTGTCT GTGTCAGATG AGGCCTGCGC AGAAGTGAAC 
GGCTTGAAGG CCTCTCGCTT CTTCAGCATG GCTGAGAGAC TGAATACGGG TTCTGAGCCC 
TTCATCTGGC TCTTCATCCG CCCACCGTGG TCAGTGTCCG GAAGCTGCTG CAAGGCTGTG 
GTTCATGACA GCCTCCGAGC GGAGTGTCAG TTACAGAGAA GTCATAAAGC TTCCATACTA 
CACTGCTTGG GAAGGGTGCT AAATCTTTTT GAGGACGAAG AGAAAAGGAA GCAGGCGCGT 
AGCCTTTTGG AAGAGTCTTC TCGTCAGGGA TGCTTAATCA GCTCATACCT CCTTTGGGAA 
AGCGACAGAA AGGTGGATAT GTCCGACCCC GGACGATGCC TCCACAGCTT CCGGAAACTC 
AGGGACTACG CTGCGAAAGG CTGCTGGGAA GCACAGCTGG CTTTGGCCAA AGCCTGTGCA 
GGTGGAAGCC AACTTGGACT GGAAGGGAAG GCCTGCAGTG AGTCGGTCTG CCAGCTCTTC 
CAGGCCTCCC AGGCTGTCAA CAAGCAGCAG ATATTCTCTG TGCAGAAGGG GCTCAGCGAC 
ACCATGAGGT ACATTCTAAT CGACTGGTTG GTGGAAGTTG CCACAATGAA GGACTTTACA 
AGCCTGTGTC TTCACCTGAC TGTGGAGTGT GTAGACCGGT ACCTGCGAAG GAGGCTGGTC 
CCCAGATACA AGCTGCAGCT CCTGGGTATT GCCTGCATGG TCATCTGTAC CCGGTTCATC 
AGCAAAGAGA TTCTGACGAT TAGAGAGGCT GTGTGGCTCA CAGACAACAC GTATAAATAC 
GAGGACTTGG TGCGCGTGAT GGGGGAGATC ATTTCCGCCC TGGAAGGGAA GATTCGGATC 
CCTACCGTGG TTGACTATAA AGAGGTCCTG CTGACACTGG TCCCCGTCGC ACCCAGAACC 
CAGCACCTGT GCAGCTTCCT CTGTGAGCTC ACCCTGCTGC ACACCAGCCT GTCCATCTAT 
GCCCCAGCCC GCCTGGCCTC TGCGGCCCTT CTCCTGGCCA GACTCATGCA TGGGCAGACA 
CAGCCCTGGA CCACTCATCT GTGGGACCTC ACTGGCTTCT CCTACAGTGA CCTCGTACCC 
TGTGTCCTGA GCCTTCATAA GAAGTGTTTC CACGATGATG CACCCAAAGA CTACAGACAA 
GTCTCTCTGA CGGCCGTGAA GCAGAGGTTC GAGGATAAGT GCTATGAGGA AATCAGCCGG 
GAAGAGGTGC TGAGCTATGC TGACTTGTGC AGTACAATAG GAGTAAAACA GGAAAGCCCA 
GAGCCCCCAT CTTTCCCTAG CTCAGGGGAG ATCCACACCT TCCTCAGCTC GCCCTCCGGG 
AGGAGGAGCA AACGGAAGCG AGAAAACAGC CTTCAGGAGG ACAGAGGCAG CTTTGTCACC 
ACACCCACCG CAGAGCTATC GAATCAGGAG GAGACACTGC TAGGCAGCCT TCTGGACTGG 
AGCCTGGAGT GCTGCTCTGG CTATGAGGGA GACCAGGAGA GCGAAGGCGA GAAGGAGGGT 
GATGTCACAG CTCCCAGCAG ACTCCTTGAT GTCACTGTGG TCTACCTGAA CCCAGAAGAA 
CATTGCTGCC AGGAGTCCAG TGATGAGGAG GCCTGGCCAG AGGACAAGAT CCACCCAGCA 
CCAGGCACCC AGGCACCTCC AGCCTCAGCT CCCAGGCCCC TTCTCTGCAA TCGGGGAGAT 
CGGGCCAAGG ACATTACGAC CTCAGGATAC TCCTCTGTTA GCAGCTCGAG TCCCATAAGC 
TCCCTGGATG GTGGCATGGG GGGCTCTCCG CAATCTACCT CAGTGCTCTC TGTGGGCAGC 
CACTCAAGCA CAAAGCCTTG CCACCATCAG GCCAAGAAGT CATGTTTACA GTGTCGTCCC 
CCAAACTCCC CAGAGAGCGG TGTTCACCAG CAGCCTGTAA AGCGCCAAAA CCTGTCTGTA 
CACAGTGACA AGGACATGCA CCTGGCTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells.
Immunity43(4)690-702(2015 Oct)
Ray JP,Staron MM,Shyer JA,Ho PC,Marshall HD,Gray SM,Laidlaw BJ,Araki K,Ahmed R,Kaech SM,Craft J

Congenital asplenia in mice and humans with mutations in a Pbx/Nkx2-5/p15 module.
Developmental cell22(5)913-26(2012 May)
Koss M,Bolze A,Brendolan A,Saggese M,Capellini TD,Bojilova E,Boisson B,Prall OW,Elliott DA,Solloway M,Lenti E,Hidaka C,Chang CP,Mahlaoui N,Harvey RP,Casanova JL,Selleri L

The protein kinase KIS impacts gene expression during development and fear conditioning in adult mice.
PloS one7(8)e43946(2012)
Manceau V,Kremmer E,Nabel EG,Maucuer A

SCF(Cyclin F) controls centrosome homeostasis and mitotic fidelity through CP110 degradation.
Nature466(7302)138-42(2010 Jul)
D'Angiolella V,Donato V,Vijayakumar S,Saraf A,Florens L,Washburn MP,Dynlacht B,Pagano M

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Ccnf cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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