ccnf cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ccnf
Entrez Gene ID	266981
Full Name	cyclin F
Synonyms	cb443,zgc:77240
General protein information	Preferred Names cyclin F Names cyclin-F
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_207048.1	NP_996931.1	cyclin-F

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ccnf	NP_001093944.1
Xenopus tropicalis (tropical clawed frog)	ccnf	NP_001011083.1
Homo sapiens (human)	CCNF	NP_001752.2
Mus musculus (house mouse)	Ccnf	NP_031660.3
Danio rerio (zebrafish)	ccnf	NP_996931.1
Bos taurus (cattle)	CCNF	NP_001092340.1
	CCNF	XP_001085466.1
Pan troglodytes (chimpanzee)	CCNF	XP_510746.2
Canis lupus familiaris (dog)	CCNF	XP_005621742.1
Gallus gallus (chicken)	CCNF	XP_415043.4

Gene Ontology
Bibliography

Related articles in PubMed

Expression of ALS/FTD-linked mutant CCNF in zebrafish leads to increased cell death in the spinal cord and an aberrant motor phenotype.
Hogan AL, Don EK, Rayner SL, Lee A, Laird AS, Watchon M, Winnick C, Tarr IS, Morsch M, Fifita JA, Gwee SSL, Formella I, Hortle E, Yuan KC, Molloy MP, Williams KL, Nicholson GA, Chung RS, Blair IP, Cole NJ
Human molecular genetics26(14)2616-2626(2017 Jul)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Telomerase Is Essential for Zebrafish Heart Regeneration.
Bednarek D, Gonz?lez-Rosa JM, Guzm?n-Mart?nez G, Guti?rrez-Guti?rrez ?, Aguado T, S?nchez-Ferrer C, Marques IJ, Galardi-Castilla M, de Diego I, G?mez MJ, Cort?s A, Zapata A, Jim?nez-Borreguero LJ, Mercader N, Flores I
Cell reports12(10)1691-703(2015 Sep)

Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis.
Recher G, Jouralet J, Brombin A, Heuz? A, Mugniery E, Hermel JM, Desnoulez S, Savy T, Herbomel P, Bourrat F, Peyri?ras N, Jamen F, Joly JS
Development (Cambridge, England)140(24)4860-9(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ccnf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ccnf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00542	NM_207048.1 Latest version!	Danio rerio cyclin F (ccnf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ccnf ( NM_207048.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_996931.1 Danio rerio cyclin F (ccnf), mRNA.

Price & Availability↑

CloneID	ODa00542
Clone ID Related Accession (Same CDS sequence)	NM_207048.1
Accession Version	NM_207048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Danio rerio(zebrafish)
Product	cyclin-F
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC066425.1. ##Evidence-Data-START## Transcript exon combination :: BC066425.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168448, SAMEA4476739 [ECO:0000348] ##Evidence-Data-END##

ATGAAAGCGG GCGCGCTCCA CTGTCGATGT GCAAAATGTT TTTCTCTCCC TGTGCGGAAG 
CGGGTGCGAA AGCGAGCATC TGCCGTCTCG CTGCTTTCCC TGCCTGAAGA GCTTCTGGTG 
TTCGTCCTCC AGTGTCTCTC TGCCGAGGAT CTCCTCTCCG TCAGAGCTGT TCATTCTCAT 
CTTTGTGACA TTATCGATAC TAACGCGAGT ATCTGGGCAC GAGTGAGTTT CAAAGACCGC 
TGGCCGGCTC CTGATACCGT TTGGCTCTTT GAGAGGGCTG CGGAGAAAGG GAACTTTGAA 
GCTGCTGTGA AACTAGGAAT TGCATATTTG TACAATGAAG GACCATTACT CAGCGAGGAG 
GGTCGTGCTG ATCTGTGTGG CCGAATGGCA TCTCGGTATC TGAGCCTGTC CGAGAGCCTC 
AGGTCCCCGC AGGCAGAGCC CTTCATCTGG CTGTTCATCC GGCCGCCCTG GTCCGTGTCC 
GGCAGCTGCT GCAAGGCTGT AGTGTTTGAT CGACTCCAGG CAGAGTGTCA GACCAGCCCG 
GGACGAAAAG GGACGTTACT GTACTGCCTG GCCCGAGTCC TTCAGCTGTT TGACGATGAG 
GAGAAGCGTG ATGAAGCGGA GCTCATGCTG AAGGAGTCCT CCAGATGCGG CAGTCTGCAG 
AGCTCCTACC TGCTGTGGGC AATCAGCCGT GCCTCATCGA CGGCCGACCC CGGCAGGTAT 
CTGCAGTGCA TGCGCACACT CAGGGACTAC GCTGCTAGAG GATGCTGGGA AGCTCAGCTG 
GCGTTTGTGA AGTCGTGTGG ATCTGCTAAC CCTTTGGGTC TGGAGCCGCG GGCCTGCAGT 
GAACTCGTGT CCCAGTTTTT CCAGACGGGA CCCTCAGCCC TTCGGTACCA GCCACATCTG 
CAGGGACAGG ACATCACCAC CAAGAGGTAT ATTCTGGTGG ACTGGTTGGT GGAAGTGACC 
ACCACGAAGG ACTTCTCCAG TCAGGTGCTT CACGTGACCA TTAGCTGTGT GGACCGATAT 
CTGCACCTGC GCTCCGTGCC AAAGGCCCAG TTGCAGCTCC TGGGCATCGC CTGCATGGTC 
ATCTGCACCC GATTCATCAG CAAGGAGATC CTGACCATCC GTGAGGCGGT TTGGCTCACA 
GATAATACCT ATCAGTATGA AGACCTGGTG CACATGATGG GCGAGGTTAT CTCTGTGCTG 
GACGGAAAGA TTCGGACCCC TACAGTGTTG GATTATGGGG AGGTGCTGCT GTCGTTGCTG 
CCGGTGGAGC GTCGAACTGC TCACCTCTTC AGCTACATCT GTGAGCTGTC TCTGCTCTGC 
TCACCCGCCA CTGTTCCCGG ACCAGCCCGA CTCGCCAGCG CCATCCTGCT GCTCACCAGA 
GCGCTGCACA ATTACGTTCC TGTGTGGCCC GTCCAGCTGG AGGAAAACAC TGGCTTCTCC 
AAGCAAGATC TGGTCTCTTG TGCTTTAACG CTCTACATCA AATGTTTTGG TCAAGATGTG 
CCCAAAGACT ACAGACACGT GTCATTAACT GGAGTCAAGC AAAGGTTTGA GGATGATTCG 
TATCAGCAGA TCAGCAAAGA CACGGTGATG GGCTTTAAGG AGCTGTGTCA TGTGCTGGAG 
GTGCCGGAGG TGGAGCCGCA GGTGGAGGTG TCCAGTACCA GTGGTCAGAT CACAGAGATG 
CACACCTTCC TGTCTTCACC CACCAGCAGC AGCAAGAGGA GGAGAGCCGA CAGCATGCAG 
GCCCACCGAG GTGCTTTTGT GGCCACACCC ACTGCAGAGT TGTCCAATCA GGAGGAGACC 
CTGCTGGGAG ACTTTCTGGA TTGGAGCCTG GACACTTCCT GTTCTGGATA CGAGGGAGAC 
CGTGAGAGCG AGGGAGAGAA GGATGGAGAA ATCTCCACAA TGGAGGTGCA GATGGAGCTG 
CCGCTGGATT GCGCTGACAG ACAGACGCAC TGCTGCCTGT TATCAAGCGA CGACACCAGT 
CTGTGTGAAG AAGACGAGCA GGAGCCTCCA ACCGGCGGTA GCAAACCCTG GACACGCCAT 
CTTTCCTCTT CCTCCACCTC CTCCTCATCC TCCTCCTGCG GCTTTTGCAC AGACCGCCAC 
AGCTCCGGCT ACTCCTCCAT TCAGAGTTTC CCCAGTCCAA CAGGGTCCTC CGCTCTGGTT 
TCCCCACAGA ATCCACCTGG TGTTCCTTCG CAAAGGATTC GCCGTCAGGT CAAGAGGAAA 
AACACGGCAG CTCACAGCGC GGGAGAAGCA GAACAAGAGG ACGACGCAGC AAACCTAGCC 
TTTCTGAGCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996931.1
CDS	122..2416
Translation	MKAGALHCRCAKCFSLPVRKRVRKRASAVSLLSLPEELLVFVLQCLSAEDLLSVRAVHSHLCDIIDTNASIWARVSFKDRWPAPDTVWLFERAAEKGNFEAAVKLGIAYLYNEGPLLSEEGRADLCGRMASRYLSLSESLRSPQAEPFIWLFIRPPWSVSGSCCKAVVFDRLQAECQTSPGRKGTLLYCLARVLQLFDDEEKRDEAELMLKESSRCGSLQSSYLLWAISRASSTADPGRYLQCMRTLRDYAARGCWEAQLAFVKSCGSANPLGLEPRACSELVSQFFQTGPSALRYQPHLQGQDITTKRYILVDWLVEVTTTKDFSSQVLHVTISCVDRYLHLRSVPKAQLQLLGIACMVICTRFISKEILTIREAVWLTDNTYQYEDLVHMMGEVISVLDGKIRTPTVLDYGEVLLSLLPVERRTAHLFSYICELSLLCSPATVPGPARLASAILLLTRALHNYVPVWPVQLEENTGFSKQDLVSCALTLYIKCFGQDVPKDYRHVSLTGVKQRFEDDSYQQISKDTVMGFKELCHVLEVPEVEPQVEVSSTSGQITEMHTFLSSPTSSSKRRRADSMQAHRGAFVATPTAELSNQEETLLGDFLDWSLDTSCSGYEGDRESEGEKDGEISTMEVQMELPLDCADRQTHCCLLSSDDTSLCEEDEQEPPTGGSKPWTRHLSSSSTSSSSSSCGFCTDRHSSGYSSIQSFPSPTGSSALVSPQNPPGVPSQRIRRQVKRKNTAAHSAGEAEQEDDAANLAFLSF

Target ORF information:

RefSeq Version	NM_207048.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cyclin F (ccnf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207048.1

ORF Insert Sequence:

ATGAAAGCGG GCGCGCTCCA CTGTCGATGT GCAAAATGTT TTTCTCTCCC TGTGCGGAAG 
CGGGTGCGAA AGCGAGCATC TGCCGTCTCG CTGCTTTCCC TGCCTGAAGA GCTTCTGGTG 
TTCGTCCTCC AGTGTCTCTC TGCCGAGGAT CTCCTCTCCG TCAGAGCTGT TCATTCTCAT 
CTTTGTGACA TTATCGATAC TAACGCGAGT ATCTGGGCAC GAGTGAGTTT CAAAGACCGC 
TGGCCGGCTC CTGATACCGT TTGGCTCTTT GAGAGGGCTG CGGAGAAAGG GAACTTTGAA 
GCTGCTGTGA AACTAGGAAT TGCATATTTG TACAATGAAG GACCATTACT CAGCGAGGAG 
GGTCGTGCTG ATCTGTGTGG CCGAATGGCA TCTCGGTATC TGAGCCTGTC CGAGAGCCTC 
AGGTCCCCGC AGGCAGAGCC CTTCATCTGG CTGTTCATCC GGCCGCCCTG GTCCGTGTCC 
GGCAGCTGCT GCAAGGCTGT AGTGTTTGAT CGACTCCAGG CAGAGTGTCA GACCAGCCCG 
GGACGAAAAG GGACGTTACT GTACTGCCTG GCCCGAGTCC TTCAGCTGTT TGACGATGAG 
GAGAAGCGTG ATGAAGCGGA GCTCATGCTG AAGGAGTCCT CCAGATGCGG CAGTCTGCAG 
AGCTCCTACC TGCTGTGGGC AATCAGCCGT GCCTCATCGA CGGCCGACCC CGGCAGGTAT 
CTGCAGTGCA TGCGCACACT CAGGGACTAC GCTGCTAGAG GATGCTGGGA AGCTCAGCTG 
GCGTTTGTGA AGTCGTGTGG ATCTGCTAAC CCTTTGGGTC TGGAGCCGCG GGCCTGCAGT 
GAACTCGTGT CCCAGTTTTT CCAGACGGGA CCCTCAGCCC TTCGGTACCA GCCACATCTG 
CAGGGACAGG ACATCACCAC CAAGAGGTAT ATTCTGGTGG ACTGGTTGGT GGAAGTGACC 
ACCACGAAGG ACTTCTCCAG TCAGGTGCTT CACGTGACCA TTAGCTGTGT GGACCGATAT 
CTGCACCTGC GCTCCGTGCC AAAGGCCCAG TTGCAGCTCC TGGGCATCGC CTGCATGGTC 
ATCTGCACCC GATTCATCAG CAAGGAGATC CTGACCATCC GTGAGGCGGT TTGGCTCACA 
GATAATACCT ATCAGTATGA AGACCTGGTG CACATGATGG GCGAGGTTAT CTCTGTGCTG 
GACGGAAAGA TTCGGACCCC TACAGTGTTG GATTATGGGG AGGTGCTGCT GTCGTTGCTG 
CCGGTGGAGC GTCGAACTGC TCACCTCTTC AGCTACATCT GTGAGCTGTC TCTGCTCTGC 
TCACCCGCCA CTGTTCCCGG ACCAGCCCGA CTCGCCAGCG CCATCCTGCT GCTCACCAGA 
GCGCTGCACA ATTACGTTCC TGTGTGGCCC GTCCAGCTGG AGGAAAACAC TGGCTTCTCC 
AAGCAAGATC TGGTCTCTTG TGCTTTAACG CTCTACATCA AATGTTTTGG TCAAGATGTG 
CCCAAAGACT ACAGACACGT GTCATTAACT GGAGTCAAGC AAAGGTTTGA GGATGATTCG 
TATCAGCAGA TCAGCAAAGA CACGGTGATG GGCTTTAAGG AGCTGTGTCA TGTGCTGGAG 
GTGCCGGAGG TGGAGCCGCA GGTGGAGGTG TCCAGTACCA GTGGTCAGAT CACAGAGATG 
CACACCTTCC TGTCTTCACC CACCAGCAGC AGCAAGAGGA GGAGAGCCGA CAGCATGCAG 
GCCCACCGAG GTGCTTTTGT GGCCACACCC ACTGCAGAGT TGTCCAATCA GGAGGAGACC 
CTGCTGGGAG ACTTTCTGGA TTGGAGCCTG GACACTTCCT GTTCTGGATA CGAGGGAGAC 
CGTGAGAGCG AGGGAGAGAA GGATGGAGAA ATCTCCACAA TGGAGGTGCA GATGGAGCTG 
CCGCTGGATT GCGCTGACAG ACAGACGCAC TGCTGCCTGT TATCAAGCGA CGACACCAGT 
CTGTGTGAAG AAGACGAGCA GGAGCCTCCA ACCGGCGGTA GCAAACCCTG GACACGCCAT 
CTTTCCTCTT CCTCCACCTC CTCCTCATCC TCCTCCTGCG GCTTTTGCAC AGACCGCCAC 
AGCTCCGGCT ACTCCTCCAT TCAGAGTTTC CCCAGTCCAA CAGGGTCCTC CGCTCTGGTT 
TCCCCACAGA ATCCACCTGG TGTTCCTTCG CAAAGGATTC GCCGTCAGGT CAAGAGGAAA 
AACACGGCAG CTCACAGCGC GGGAGAAGCA GAACAAGAGG ACGACGCAGC AAACCTAGCC 
TTTCTGAGCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of ALS/FTD-linked mutant CCNF in zebrafish leads to increased cell death in the spinal cord and an aberrant motor phenotype.
Human molecular genetics26(14)2616-2626(2017 Jul)
Hogan AL,Don EK,Rayner SL,Lee A,Laird AS,Watchon M,Winnick C,Tarr IS,Morsch M,Fifita JA,Gwee SSL,Formella I,Hortle E,Yuan KC,Molloy MP,Williams KL,Nicholson GA,Chung RS,Blair IP,Cole NJ

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Telomerase Is Essential for Zebrafish Heart Regeneration.
Cell reports12(10)1691-703(2015 Sep)
Bednarek D,Gonz?lez-Rosa JM,Guzm?n-Mart?nez G,Guti?rrez-Guti?rrez ?,Aguado T,S?nchez-Ferrer C,Marques IJ,Galardi-Castilla M,de Diego I,G?mez MJ,Cort?s A,Zapata A,Jim?nez-Borreguero LJ,Mercader N,Flores I

Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis.
Development (Cambridge, England)140(24)4860-9(2013 Dec)
Recher G,Jouralet J,Brombin A,Heuz? A,Mugniery E,Hermel JM,Desnoulez S,Savy T,Herbomel P,Bourrat F,Peyri?ras N,Jamen F,Joly JS

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ccnf cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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