Brca1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Brca1
Entrez Gene ID	12189
Full Name	breast cancer 1, early onset
General protein information	Preferred Names breast cancer 1, early onset Names breast cancer type 1 susceptibility protein homolog RING-type E3 ubiquitin transferase BRCA1 breast cancer associated 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 65.18 cM\|11 D

mRNA and Protein(s)

mRNA	Protein	Name
XM_006532064.3	XP_006532127.1	breast cancer type 1 susceptibility protein homolog isoform X1
XM_030245495.1	XP_030101355.1	breast cancer type 1 susceptibility protein homolog isoform X1
XM_006532064.4	XP_006532127.1	breast cancer type 1 susceptibility protein homolog isoform X2
NM_009764.3	NP_033894.3	breast cancer type 1 susceptibility protein homolog
XM_006532068.2	XP_006532131.1	breast cancer type 1 susceptibility protein homolog isoform X3
XM_017314233.1	XP_017169722.1	breast cancer type 1 susceptibility protein homolog isoform X2
XM_017314233.2	XP_017169722.1	breast cancer type 1 susceptibility protein homolog isoform X3
XM_006532068.3	XP_006532131.1	breast cancer type 1 susceptibility protein homolog isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	BRCA1	NP_009231.2
Mus musculus (house mouse)	Brca1	NP_033894.3
Gallus gallus (chicken)	BRCA1	NP_989500.1
	BRCA1	NP_001108421.1
Canis lupus familiaris (dog)	BRCA1	NP_001013434.1
Bos taurus (cattle)	BRCA1	NP_848668.1
Rattus norvegicus (Norway rat)	Brca1	NP_036646.1
Pan troglodytes (chimpanzee)	BRCA1	NP_001038958.1

Gene Ontology

The following Brca1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Brca1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu45463	XM_006532068.3 Latest version!	Mus musculus breast cancer 1, early onset (Brca1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu77714	XM_017314233.2 Latest version!	Mus musculus breast cancer 1, early onset (Brca1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu20079	XM_030245495.1 Latest version!	Mus musculus breast cancer 1, early onset (Brca1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu45459	XM_006532064.4 Latest version!	Mus musculus breast cancer 1, early onset (Brca1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu20079	NM_009764.3 Latest version!	Mus musculus breast cancer 1, early onset (Brca1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OMu45459	XM_006532064.3	Mus musculus breast cancer 1, early onset (Brca1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu77714	XM_017314233.1	Mus musculus breast cancer 1, early onset (Brca1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu45463	XM_006532068.2	Mus musculus breast cancer 1, early onset (Brca1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Brca1 ( XM_006532068.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532131.1 Mus musculus breast cancer 1, early onset (Brca1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu45463
Clone ID Related Accession (Same CDS sequence)	XM_006532068.2 , XM_006532068.3
Accession Version	XM_006532068.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	breast cancer type 1 susceptibility protein homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532068.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAT CTGCCGTCCA AATTCAAGAA GTACAAAATG TCCTTCATGC TATGCAGAAA 
ATCTTAGAGT GTCCGATCTG TTTGGAACTG ATCAAAGAAC CTGTTTCCAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTTAACC AGAAGAAAGG GCCTTCACAA 
TGTCCTTTGT GTAAGAATGA GATAACCAAA AGGAGCCTAC AGGGAAGCAC AAGGTTTAGT 
CAGCTTGCTG AAGAGCTGCT GAGAATAATG GCTGCTTTTG AGCTTGACAC GGGAATGCAG 
CTTACAAATG GTTTTAGTTT TTCAAAAAAG AGAAATAATT CTTGTGAGCG TTTGAATGAG 
GAGGCGTCGA TCATCCAGAG CGTGGGCTAC CGGAACCGTG TCAGAAGGCT TCCCCAGGTC 
GAACCTGGAA ATGCCACCTT GAAGGACAGC CTAGGTGTCC AGCTGTCTAA CCTTGGAATC 
GTGAGATCAG TGAAGAAAAA CAGGCAGACC CAACCTCGAA AGAAATCTGT CTACATTGAA 
CTAGACTCTG ATTCTTCTGA AGAGACAGTA ACTAAGCCAG GTGATTGCAG TGTGAGAGAC 
CAGGAATTGT TACAGACCGC CCCTCAAGAA GCTGGAGATG AAGGCAAGCT GCACTCTGCA 
GAAGAGGAGG CATCCGGATA CGAGAGTGAA ACAAACCTTT CTGAAGACTG CTCGCAGAGT 
GATATTTTAA CCACTCAGCA GCGGGCGACC ATGAAGTATA ACCTGATAAA GCTGCAGCAG 
GAAATGGCTC ACCTGGAAGC TGTGCTGGAG CAGCGTGGGA ACCAGCCTTC TGGCCACTCC 
CCTTCCCTCC TAGCGGACCC TTGTGCCCTG GAAGACCTGC CAGATCTGGA ACCAAACATG 
TCAGGAGCAG CAATTTTAAC TTCAAAGAAC ATTAATGAGA ATCCTGTAAG CCAAAATTTG 
AAGAGCGCTT GTGATGACAA ATTCCAACTA CAACATCTGG AGGGTCCCAC CAGTGGAGAT 
GACGAGTCAG GGATGGGAAG GCCTTCCCCT TTTAAATCTC CGTTGGCAGG CAGTAGGGGC 
TCTGCACATG GCTGCTCTAG GCATCTTCAA AAGAGAAACT CCCCCTCTCA GGAGGAGCTC 
CTCCAGCCTG CTGGATCAGA GGCGTCATCT GAGCCACACA ATTCAACAGG GCAGTCTTGC 
CTGCCAAGGC GAGAGCTAGA AGGAACCCCA TACCTGGGAT CTGGAATCAG CCTTTTCTCT 
AGTAGAGACC CCGAATCTGA GTCCCCTAAA GAGCCAGCCC ACATTGGCAC CACACCAGCT 
TCAACCTCTG CACTGAAAAT ACCCCAAGGT CAAGTTGCTT TCCGGAGTGC AGCTGCTGCT 
GGTGCTGATA AAGCAGTGGT AGGAATTGTG AGCAAGATAA AGCCGGAATT GACATCTTCA 
GAAGAAAGAG CGGATAGAGA CATATCCATG GTGGTGTCAG GCTTGACCCC CAAAGAAGTA 
ATGACCGTGC AAAAGTTTGC TGAAAAATAC CGCCTCACTT TAACTGACGC AATTACTGAG 
GAGACTACAC ATGTAATTAT AAAAACAGAT GCGGAGTTTG TGTGTGAGCG GACACTGAAA 
TATTTTCTGG GCATTGCAGG AGGAAAGTGG ATAGTTAGCT ATTCATGGGT GGTCCGGTCT 
ATCCAAGAAA GAAGACTTCT GAATGTGCAT GAATTTGAAG TCAAAGGAGA TGTTGTGACT 
GGAAGAAATC ACCAAGGTCC AAGGCGATCC AGAGAATCCC GGGAAAAGCT CTTCAAGGGC 
CTACAGGTCT ATTGTTGTGA GCCCTTCACC AACATGCCCA AAGATGAGCT GGAGAGGATG 
CTGCAGCTGT GTGGGGCTTC CGTGGTGAAG GAGCTTCCAT CGCTCACCCA TGACACAGGT 
GCTCATCTAG TTGTGATCGT GCAGCCAAGC GCCTGGACAG AAGACAGCAA CTGCCCAGAT 
ATTGGGCAGC TGTGCAAGGC ACGTCTTGTG ATGTGGGACT GGGTGTTGGA CAGTCTATCC 
AGCTACCGGT GTCGGGATCT GGATGCCTAC CTGGTACAGA ATATCACCTG TGACAGTAGT 
GAGCCACAAG ACTCCAATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532131.1
CDS	194..2317
Translation	MDLSAVQIQEVQNVLHAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNEITKRSLQGSTRFSQLAEELLRIMAAFELDTGMQLTNGFSFSKKRNNSCERLNEEASIIQSVGYRNRVRRLPQVEPGNATLKDSLGVQLSNLGIVRSVKKNRQTQPRKKSVYIELDSDSSEETVTKPGDCSVRDQELLQTAPQEAGDEGKLHSAEEEASGYESETNLSEDCSQSDILTTQQRATMKYNLIKLQQEMAHLEAVLEQRGNQPSGHSPSLLADPCALEDLPDLEPNMSGAAILTSKNINENPVSQNLKSACDDKFQLQHLEGPTSGDDESGMGRPSPFKSPLAGSRGSAHGCSRHLQKRNSPSQEELLQPAGSEASSEPHNSTGQSCLPRRELEGTPYLGSGISLFSSRDPESESPKEPAHIGTTPASTSALKIPQGQVAFRSAAAAGADKAVVGIVSKIKPELTSSEERADRDISMVVSGLTPKEVMTVQKFAEKYRLTLTDAITEETTHVIIKTDAEFVCERTLKYFLGIAGGKWIVSYSWVVRSIQERRLLNVHEFEVKGDVVTGRNHQGPRRSRESREKLFKGLQVYCCEPFTNMPKDELERMLQLCGASVVKELPSLTHDTGAHLVVIVQPSAWTEDSNCPDIGQLCKARLVMWDWVLDSLSSYRCRDLDAYLVQNITCDSSEPQDSND

Target ORF information:

RefSeq Version	XM_006532068.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus breast cancer 1, early onset (Brca1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532068.2

ORF Insert Sequence:

ATGGATTTAT CTGCCGTCCA AATTCAAGAA GTACAAAATG TCCTTCATGC TATGCAGAAA 
ATCTTAGAGT GTCCGATCTG TTTGGAACTG ATCAAAGAAC CTGTTTCCAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTTAACC AGAAGAAAGG GCCTTCACAA 
TGTCCTTTGT GTAAGAATGA GATAACCAAA AGGAGCCTAC AGGGAAGCAC AAGGTTTAGT 
CAGCTTGCTG AAGAGCTGCT GAGAATAATG GCTGCTTTTG AGCTTGACAC GGGAATGCAG 
CTTACAAATG GTTTTAGTTT TTCAAAAAAG AGAAATAATT CTTGTGAGCG TTTGAATGAG 
GAGGCGTCGA TCATCCAGAG CGTGGGCTAC CGGAACCGTG TCAGAAGGCT TCCCCAGGTC 
GAACCTGGAA ATGCCACCTT GAAGGACAGC CTAGGTGTCC AGCTGTCTAA CCTTGGAATC 
GTGAGATCAG TGAAGAAAAA CAGGCAGACC CAACCTCGAA AGAAATCTGT CTACATTGAA 
CTAGACTCTG ATTCTTCTGA AGAGACAGTA ACTAAGCCAG GTGATTGCAG TGTGAGAGAC 
CAGGAATTGT TACAGACCGC CCCTCAAGAA GCTGGAGATG AAGGCAAGCT GCACTCTGCA 
GAAGAGGAGG CATCCGGATA CGAGAGTGAA ACAAACCTTT CTGAAGACTG CTCGCAGAGT 
GATATTTTAA CCACTCAGCA GCGGGCGACC ATGAAGTATA ACCTGATAAA GCTGCAGCAG 
GAAATGGCTC ACCTGGAAGC TGTGCTGGAG CAGCGTGGGA ACCAGCCTTC TGGCCACTCC 
CCTTCCCTCC TAGCGGACCC TTGTGCCCTG GAAGACCTGC CAGATCTGGA ACCAAACATG 
TCAGGAGCAG CAATTTTAAC TTCAAAGAAC ATTAATGAGA ATCCTGTAAG CCAAAATTTG 
AAGAGCGCTT GTGATGACAA ATTCCAACTA CAACATCTGG AGGGTCCCAC CAGTGGAGAT 
GACGAGTCAG GGATGGGAAG GCCTTCCCCT TTTAAATCTC CGTTGGCAGG CAGTAGGGGC 
TCTGCACATG GCTGCTCTAG GCATCTTCAA AAGAGAAACT CCCCCTCTCA GGAGGAGCTC 
CTCCAGCCTG CTGGATCAGA GGCGTCATCT GAGCCACACA ATTCAACAGG GCAGTCTTGC 
CTGCCAAGGC GAGAGCTAGA AGGAACCCCA TACCTGGGAT CTGGAATCAG CCTTTTCTCT 
AGTAGAGACC CCGAATCTGA GTCCCCTAAA GAGCCAGCCC ACATTGGCAC CACACCAGCT 
TCAACCTCTG CACTGAAAAT ACCCCAAGGT CAAGTTGCTT TCCGGAGTGC AGCTGCTGCT 
GGTGCTGATA AAGCAGTGGT AGGAATTGTG AGCAAGATAA AGCCGGAATT GACATCTTCA 
GAAGAAAGAG CGGATAGAGA CATATCCATG GTGGTGTCAG GCTTGACCCC CAAAGAAGTA 
ATGACCGTGC AAAAGTTTGC TGAAAAATAC CGCCTCACTT TAACTGACGC AATTACTGAG 
GAGACTACAC ATGTAATTAT AAAAACAGAT GCGGAGTTTG TGTGTGAGCG GACACTGAAA 
TATTTTCTGG GCATTGCAGG AGGAAAGTGG ATAGTTAGCT ATTCATGGGT GGTCCGGTCT 
ATCCAAGAAA GAAGACTTCT GAATGTGCAT GAATTTGAAG TCAAAGGAGA TGTTGTGACT 
GGAAGAAATC ACCAAGGTCC AAGGCGATCC AGAGAATCCC GGGAAAAGCT CTTCAAGGGC 
CTACAGGTCT ATTGTTGTGA GCCCTTCACC AACATGCCCA AAGATGAGCT GGAGAGGATG 
CTGCAGCTGT GTGGGGCTTC CGTGGTGAAG GAGCTTCCAT CGCTCACCCA TGACACAGGT 
GCTCATCTAG TTGTGATCGT GCAGCCAAGC GCCTGGACAG AAGACAGCAA CTGCCCAGAT 
ATTGGGCAGC TGTGCAAGGC ACGTCTTGTG ATGTGGGACT GGGTGTTGGA CAGTCTATCC 
AGCTACCGGT GTCGGGATCT GGATGCCTAC CTGGTACAGA ATATCACCTG TGACAGTAGT 
GAGCCACAAG ACTCCAATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brca1 ( XM_006532068.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532131.1 Mus musculus breast cancer 1, early onset (Brca1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu45463
Clone ID Related Accession (Same CDS sequence)	XM_006532068.2 , XM_006532068.3
Accession Version	XM_006532068.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	breast cancer type 1 susceptibility protein homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532068.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAT CTGCCGTCCA AATTCAAGAA GTACAAAATG TCCTTCATGC TATGCAGAAA 
ATCTTAGAGT GTCCGATCTG TTTGGAACTG ATCAAAGAAC CTGTTTCCAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTTAACC AGAAGAAAGG GCCTTCACAA 
TGTCCTTTGT GTAAGAATGA GATAACCAAA AGGAGCCTAC AGGGAAGCAC AAGGTTTAGT 
CAGCTTGCTG AAGAGCTGCT GAGAATAATG GCTGCTTTTG AGCTTGACAC GGGAATGCAG 
CTTACAAATG GTTTTAGTTT TTCAAAAAAG AGAAATAATT CTTGTGAGCG TTTGAATGAG 
GAGGCGTCGA TCATCCAGAG CGTGGGCTAC CGGAACCGTG TCAGAAGGCT TCCCCAGGTC 
GAACCTGGAA ATGCCACCTT GAAGGACAGC CTAGGTGTCC AGCTGTCTAA CCTTGGAATC 
GTGAGATCAG TGAAGAAAAA CAGGCAGACC CAACCTCGAA AGAAATCTGT CTACATTGAA 
CTAGACTCTG ATTCTTCTGA AGAGACAGTA ACTAAGCCAG GTGATTGCAG TGTGAGAGAC 
CAGGAATTGT TACAGACCGC CCCTCAAGAA GCTGGAGATG AAGGCAAGCT GCACTCTGCA 
GAAGAGGAGG CATCCGGATA CGAGAGTGAA ACAAACCTTT CTGAAGACTG CTCGCAGAGT 
GATATTTTAA CCACTCAGCA GCGGGCGACC ATGAAGTATA ACCTGATAAA GCTGCAGCAG 
GAAATGGCTC ACCTGGAAGC TGTGCTGGAG CAGCGTGGGA ACCAGCCTTC TGGCCACTCC 
CCTTCCCTCC TAGCGGACCC TTGTGCCCTG GAAGACCTGC CAGATCTGGA ACCAAACATG 
TCAGGAGCAG CAATTTTAAC TTCAAAGAAC ATTAATGAGA ATCCTGTAAG CCAAAATTTG 
AAGAGCGCTT GTGATGACAA ATTCCAACTA CAACATCTGG AGGGTCCCAC CAGTGGAGAT 
GACGAGTCAG GGATGGGAAG GCCTTCCCCT TTTAAATCTC CGTTGGCAGG CAGTAGGGGC 
TCTGCACATG GCTGCTCTAG GCATCTTCAA AAGAGAAACT CCCCCTCTCA GGAGGAGCTC 
CTCCAGCCTG CTGGATCAGA GGCGTCATCT GAGCCACACA ATTCAACAGG GCAGTCTTGC 
CTGCCAAGGC GAGAGCTAGA AGGAACCCCA TACCTGGGAT CTGGAATCAG CCTTTTCTCT 
AGTAGAGACC CCGAATCTGA GTCCCCTAAA GAGCCAGCCC ACATTGGCAC CACACCAGCT 
TCAACCTCTG CACTGAAAAT ACCCCAAGGT CAAGTTGCTT TCCGGAGTGC AGCTGCTGCT 
GGTGCTGATA AAGCAGTGGT AGGAATTGTG AGCAAGATAA AGCCGGAATT GACATCTTCA 
GAAGAAAGAG CGGATAGAGA CATATCCATG GTGGTGTCAG GCTTGACCCC CAAAGAAGTA 
ATGACCGTGC AAAAGTTTGC TGAAAAATAC CGCCTCACTT TAACTGACGC AATTACTGAG 
GAGACTACAC ATGTAATTAT AAAAACAGAT GCGGAGTTTG TGTGTGAGCG GACACTGAAA 
TATTTTCTGG GCATTGCAGG AGGAAAGTGG ATAGTTAGCT ATTCATGGGT GGTCCGGTCT 
ATCCAAGAAA GAAGACTTCT GAATGTGCAT GAATTTGAAG TCAAAGGAGA TGTTGTGACT 
GGAAGAAATC ACCAAGGTCC AAGGCGATCC AGAGAATCCC GGGAAAAGCT CTTCAAGGGC 
CTACAGGTCT ATTGTTGTGA GCCCTTCACC AACATGCCCA AAGATGAGCT GGAGAGGATG 
CTGCAGCTGT GTGGGGCTTC CGTGGTGAAG GAGCTTCCAT CGCTCACCCA TGACACAGGT 
GCTCATCTAG TTGTGATCGT GCAGCCAAGC GCCTGGACAG AAGACAGCAA CTGCCCAGAT 
ATTGGGCAGC TGTGCAAGGC ACGTCTTGTG ATGTGGGACT GGGTGTTGGA CAGTCTATCC 
AGCTACCGGT GTCGGGATCT GGATGCCTAC CTGGTACAGA ATATCACCTG TGACAGTAGT 
GAGCCACAAG ACTCCAATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532131.1
CDS	204..2327
Translation	MDLSAVQIQEVQNVLHAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNEITKRSLQGSTRFSQLAEELLRIMAAFELDTGMQLTNGFSFSKKRNNSCERLNEEASIIQSVGYRNRVRRLPQVEPGNATLKDSLGVQLSNLGIVRSVKKNRQTQPRKKSVYIELDSDSSEETVTKPGDCSVRDQELLQTAPQEAGDEGKLHSAEEEASGYESETNLSEDCSQSDILTTQQRATMKYNLIKLQQEMAHLEAVLEQRGNQPSGHSPSLLADPCALEDLPDLEPNMSGAAILTSKNINENPVSQNLKSACDDKFQLQHLEGPTSGDDESGMGRPSPFKSPLAGSRGSAHGCSRHLQKRNSPSQEELLQPAGSEASSEPHNSTGQSCLPRRELEGTPYLGSGISLFSSRDPESESPKEPAHIGTTPASTSALKIPQGQVAFRSAAAAGADKAVVGIVSKIKPELTSSEERADRDISMVVSGLTPKEVMTVQKFAEKYRLTLTDAITEETTHVIIKTDAEFVCERTLKYFLGIAGGKWIVSYSWVVRSIQERRLLNVHEFEVKGDVVTGRNHQGPRRSRESREKLFKGLQVYCCEPFTNMPKDELERMLQLCGASVVKELPSLTHDTGAHLVVIVQPSAWTEDSNCPDIGQLCKARLVMWDWVLDSLSSYRCRDLDAYLVQNITCDSSEPQDSND

Target ORF information:

RefSeq Version	XM_006532068.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus breast cancer 1, early onset (Brca1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532068.3

ORF Insert Sequence:

ATGGATTTAT CTGCCGTCCA AATTCAAGAA GTACAAAATG TCCTTCATGC TATGCAGAAA 
ATCTTAGAGT GTCCGATCTG TTTGGAACTG ATCAAAGAAC CTGTTTCCAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTTAACC AGAAGAAAGG GCCTTCACAA 
TGTCCTTTGT GTAAGAATGA GATAACCAAA AGGAGCCTAC AGGGAAGCAC AAGGTTTAGT 
CAGCTTGCTG AAGAGCTGCT GAGAATAATG GCTGCTTTTG AGCTTGACAC GGGAATGCAG 
CTTACAAATG GTTTTAGTTT TTCAAAAAAG AGAAATAATT CTTGTGAGCG TTTGAATGAG 
GAGGCGTCGA TCATCCAGAG CGTGGGCTAC CGGAACCGTG TCAGAAGGCT TCCCCAGGTC 
GAACCTGGAA ATGCCACCTT GAAGGACAGC CTAGGTGTCC AGCTGTCTAA CCTTGGAATC 
GTGAGATCAG TGAAGAAAAA CAGGCAGACC CAACCTCGAA AGAAATCTGT CTACATTGAA 
CTAGACTCTG ATTCTTCTGA AGAGACAGTA ACTAAGCCAG GTGATTGCAG TGTGAGAGAC 
CAGGAATTGT TACAGACCGC CCCTCAAGAA GCTGGAGATG AAGGCAAGCT GCACTCTGCA 
GAAGAGGAGG CATCCGGATA CGAGAGTGAA ACAAACCTTT CTGAAGACTG CTCGCAGAGT 
GATATTTTAA CCACTCAGCA GCGGGCGACC ATGAAGTATA ACCTGATAAA GCTGCAGCAG 
GAAATGGCTC ACCTGGAAGC TGTGCTGGAG CAGCGTGGGA ACCAGCCTTC TGGCCACTCC 
CCTTCCCTCC TAGCGGACCC TTGTGCCCTG GAAGACCTGC CAGATCTGGA ACCAAACATG 
TCAGGAGCAG CAATTTTAAC TTCAAAGAAC ATTAATGAGA ATCCTGTAAG CCAAAATTTG 
AAGAGCGCTT GTGATGACAA ATTCCAACTA CAACATCTGG AGGGTCCCAC CAGTGGAGAT 
GACGAGTCAG GGATGGGAAG GCCTTCCCCT TTTAAATCTC CGTTGGCAGG CAGTAGGGGC 
TCTGCACATG GCTGCTCTAG GCATCTTCAA AAGAGAAACT CCCCCTCTCA GGAGGAGCTC 
CTCCAGCCTG CTGGATCAGA GGCGTCATCT GAGCCACACA ATTCAACAGG GCAGTCTTGC 
CTGCCAAGGC GAGAGCTAGA AGGAACCCCA TACCTGGGAT CTGGAATCAG CCTTTTCTCT 
AGTAGAGACC CCGAATCTGA GTCCCCTAAA GAGCCAGCCC ACATTGGCAC CACACCAGCT 
TCAACCTCTG CACTGAAAAT ACCCCAAGGT CAAGTTGCTT TCCGGAGTGC AGCTGCTGCT 
GGTGCTGATA AAGCAGTGGT AGGAATTGTG AGCAAGATAA AGCCGGAATT GACATCTTCA 
GAAGAAAGAG CGGATAGAGA CATATCCATG GTGGTGTCAG GCTTGACCCC CAAAGAAGTA 
ATGACCGTGC AAAAGTTTGC TGAAAAATAC CGCCTCACTT TAACTGACGC AATTACTGAG 
GAGACTACAC ATGTAATTAT AAAAACAGAT GCGGAGTTTG TGTGTGAGCG GACACTGAAA 
TATTTTCTGG GCATTGCAGG AGGAAAGTGG ATAGTTAGCT ATTCATGGGT GGTCCGGTCT 
ATCCAAGAAA GAAGACTTCT GAATGTGCAT GAATTTGAAG TCAAAGGAGA TGTTGTGACT 
GGAAGAAATC ACCAAGGTCC AAGGCGATCC AGAGAATCCC GGGAAAAGCT CTTCAAGGGC 
CTACAGGTCT ATTGTTGTGA GCCCTTCACC AACATGCCCA AAGATGAGCT GGAGAGGATG 
CTGCAGCTGT GTGGGGCTTC CGTGGTGAAG GAGCTTCCAT CGCTCACCCA TGACACAGGT 
GCTCATCTAG TTGTGATCGT GCAGCCAAGC GCCTGGACAG AAGACAGCAA CTGCCCAGAT 
ATTGGGCAGC TGTGCAAGGC ACGTCTTGTG ATGTGGGACT GGGTGTTGGA CAGTCTATCC 
AGCTACCGGT GTCGGGATCT GGATGCCTAC CTGGTACAGA ATATCACCTG TGACAGTAGT 
GAGCCACAAG ACTCCAATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brca1 ( XM_017314233.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169722.1 Mus musculus breast cancer 1, early onset (Brca1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu77714
Clone ID Related Accession (Same CDS sequence)	XM_017314233.1 , XM_017314233.2
Accession Version	XM_017314233.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	breast cancer type 1 susceptibility protein homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAT CTGCCGTCCA AATTCAAGAA GTACAAAATG TCCTTCATGC TATGCAGAAA 
ATCTTAGAGT GTCCGATCTG TTTGGAACTG ATCAAAGAAC CTGTTTCCAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTTAACC AGAAGAAAGG GCCTTCACAA 
TGTCCTTTGT GTAAGAATGA GATAACCAAA AGGAGCCTAC AGGGAAGCAC AAGGTTTAGT 
CAGCTTGCTG AAGAGCTGCT GAGAATAATG GCTGCTTTTG AGCTTGACAC GGGAATGCAG 
CTTACAAATG GTTTTAGTTT TTCAAAAAAG AGAAATAATT CTTGTGAGCG TTTGAATGAG 
GAGGCGTCGA TCATCCAGAG CGTGGGCTAC CGGAACCGTG TCAGAAGGCT TCCCCAGGTC 
GAACCTGGAA ATGCCACCTT GAAGGACAGC CTAGGTGTCC AGCTGTCTAA CCTTGGAATC 
GTGAGATCAG TGAAGAAAAA CAGGCAGACC CAACCTCGAA AGAAATCTGT CTACATTGAA 
CTAGACTCTG ATTCTTCTGA AGAGACAGTA ACTAAGCCAG GTGATTGCAG TGTGAGAGAC 
CAGGAATTGT TACAGACCGC CCCTCAAGAA GCTGGAGATG AAGGCAAGCT GCACTCTGCA 
GAAGAGGCTG CTTGTGAGTT TTCTGAGGGC ATAAGAAACA TTGAACATCA TCAATGCAGT 
GATGATTTAA ACCCTACTGA GAATCATGCA ACTGAAAGGC ATCCAGAAAA ATGTCAGAGT 
ATTTCTATTT CAAATGTGTG TGTGGAGCCA TGTGGCACAG ATGCTCATGC CAGCTCATTA 
CAGCCTGAGA CCAGCAGTTT ATTGCTCATT GAAGACAGAA TGAATGCAGA AAAGGCTGAA 
TTCTGTAATA AAAGCAAACA GCCTGGCATA GCAGTGAGCC AGCAGAGCAG ATGGGCTGCA 
AGTAAAGGAA CATGTAACGA CAGGCAGGTT CCCAGCACTG GGGAAAAGGT AGGTCCAAAC 
GCTGACTCCC TTAGTGATAG AGAGAAGTGG ACTCACCCGC AAAGTCTGTG CCCTGAGAAT 
TCTGGAGCTA CCACCGATGT TCCTTGGATA ACACTAAATA GCAGCGTTCA GAAAGTTAAT 
GAGTGGTTTT CCAGAACTGG TGAAATGTTA ACTTCTGACA GCGCATCTGC CAGGAGGCAC 
GAGTCAAATG CTGAAGCAGC TGTTGTGTTG GAAGTTTCAA ACGAAGTGGA TGGGGGTTTT 
AGTTCTTCAA GGAAAACAGA CTTAGTAACC CCCGACCCCC ATCATACTTT AATGTGTAAA 
AGTGGAAGAG ACTTCTCCAA ACCAGTAGAG GATAATATCA GTGATAAAAT ATTTGGGAAA 
TCCTATCAGA GAAAGGGAAG CCGCCCTCAC CTGAACCATG TGACTGAAAT TATAGGCACA 
TTTATTACAG AACCACAGAT AACACAAGAG CAGCCCTTCA CAAATAAATT AAAACGTAAG 
AGAAGTACAT CCCTTCAACC TGAGGACTTC ATCAAGAAAG CAGATTCAGC AGGTGTTCAA 
AGGACTCCTG ACAACATAAA TCAGGGAACT GACCTAATGG AGCCAAATGA GCAAGCAGTG 
AGTACTACCA GTAACTGTCA GGAGAACAAA ATAGCAGGTA GTAATCTCCA GAAAGAGAAA 
AGCGCTCATC CAACTGAATC ATTGAGAAAG GAACCTGCTT CCACAGCAGG AGCCAAATCT 
ATAAGCAACA GTGTAAGTGA TTTGGAGGTA GAATTAAACG TCCACAGTTC AAAAGCACCT 
AAGAAAAATA GGCTGAGGAG GAAGTCTTCT ATCAGGTGTG CTCTTCCACT TGAACCAATC 
AGTAGAAATC CAAGCCCACC TACTTGTGCT GAGCTTCAAA TCGATAGTTG TGGTAGCAGT 
GAAGAAACAA AGAAAAACCA TTCCAACCAA CAGCCAGCCG GGCACCTTAG AGAGCCTCAA 
CTCATCGAAG ACACTGAACC TGCAGCGGAT GCCAAGAAGA ACGAGCCAAA TGAACACATA 
AGGAAGAGAC GTGCCAGCGA TGCTTTCCCA GAAGAGAAAT TAATGAACAA AGCTGGTTTA 
TTAACTAGCT GTTCAAGTCC TAGAAAATCT CAAGGGCCTG TCAATCCCAG CCCTCAGAGA 
ACAGGAACAG AGCAACTTGA AACACGCCAA ATGTCTGACA GTGCCAAAGA ACTCGGGGAT 
CGGGTCCTAG GAGGAGAGCC CAGTGGCAAA ACCACTGACC GATCTGAGGA GAGCACCAGC 
GTATCCTTGG TATCTGACAC TGACTACGAC ACTCAGAACA GTGTCTCAGT CCTGGACGCT 
CACACTGTCA GATATGCAAG AACAGGATCC GCTCAGTGTA TGACTCAGTT TGTAGCAAGC 
GAAAACCCCA AGGAACTCGT CCATGGCTCT AACAATGCTG GGAGTGGCAC AGAGGGTCTC 
AAGCCCCCCT TGAGACACGC GCTTAACCTC AGTCAGGAGA AAGTAGAAAT GGAAGACAGT 
GAACTTGATA CTCAGTATTT GCAGAATACA TTTCAAGTTT CAAAGCGTCA GTCATTTGCT 
TTATTTTCAA AACCTAGAAG TCCCCAAAAG GACTGTGCTC ACTCTGTGCC CTCAAAGGAA 
CTGAGTCCAA AGGTGACAGC TAAAGGTAAA CAAAAAGAAC GTCAGGGACA GGAAGAATTT 
GAAATCAGTC ACGTACAAGC AGTTGCGGCC ACAGTGGGCT TACCTGTGCC CTGTCAAGAA 
GGTAAGCTAG CTGCTGATAC AATGTGTGAT AGAGGTTGTA GGCTTTGTCC ATCATCTCAT 
TACAGAAGCG GGGAGAATGG ACTCAGCGCC ACAGGTAAAT CAGGAATTTC ACAAAACTCA 
CATTTTAAAC AATCAGTTTC TCCCATCAGG TCATCTATAA AAACTGACAA TAGGAAACCT 
CTGACAGAGG GACGATTTGA GAGACATACA TCATCAACTG AGATGGCGGT GGGAAATGAG 
AACATTCTTC AGAGTACAGT GCACACAGTT AGCCTGAATA ACAGAGGAAA TGCTTGTCAA 
GAAGCCGGCT CGGGCAGTAT TCATGAAGTA TGTTCCACTG GTGACTCCTT CCCAGGACAA 
CTAGGTAGAA ACAGAGGGCC TAAGGTGAAC ACTGTGCCTC CATTAGATAG TATGCAGCCT 
GGTGTCTGTC AGCAAAGTGT TCCTGTAAGT GATAAGTATC TTGAAATAAA AAAGCAGGAG 
GGTGAGGCTG TCTGTGCAGA CTTCTCTCCA TGTCTATTCT CAGACCATCT TGAGCAATCT 
ATGAGTGGTA AGGTTTTTCA GGTTTGCTCT GAGACACCTG ATGACCTGCT GGATGATGTT 
GAAATACAGG GACATACTAG CTTTGGTGAA GGTGACATAA TGGAGAGATC TGCTGTCTTT 
AACGGAAGCA TCCTGAGAAG GGAGTCCAGT AGGAGCCCTA GTCCTGTAAC CCATGCATCG 
AAGTCTCAGA GTCTCCACAG AGCGTCTAGG AAATTAGAAT CGTCAGAAGA GAGCGACTCC 
ACTGAGGATG AAGATCTTCC CTGCTTCCAA CACTTACTGA GCAGAATAAG CAACACACCT 
GAGCTTACCA GATGCAGCAG TGCTGTGACA CAGCGTATGC CAGAGAAAGC GGAGGGGACC 
CAAGCACCAT GGAAGGGTAG CAGCAGTGAC TGCAATAATG AGGTGATCAT GATAGAGGCA 
TCTCAGGAGC ATCAGTTTAG TGAGGATCCA AGATGCTCTG GCAGCATGTT CTCTTCACAG 
CACAGTGCTG CCCAAGGGTC AACTGCAAAT GCAAACTCCC AGGATTCCAA TTTTATTCCA 
CCTTCCAAAC AGAGGAGTCA CCAGTGTGGG AATGAGGAAG CTTTCCTAAG TGACAAGGAA 
TTGATTTCAG ATAACGAGGA AATGGCAACT TGCCTAGAAG AGGATAATGA CCAAGAAGAG 
GATAGTATAA TCCCAGATTC AGAGGCATCC GGATACGAGA GTGAAACAAA CCTTTCTGAA 
GACTGCTCGC AGAGTGATAT TTTAACCACT CAGCAGCGGG CGACCATGAA GTATAACCTG 
ATAAAGCTGC AGCAGGAAAT GGCTCACCTG GAAGCTGTGC TGGAGCAGCG TGGGAACCAG 
CCTTCTGGCC ACTCCCCTTC CCTCCTAGCG GACCCTTGTG CCCTGGAAGA CCTGCCAGAT 
CTGGAACCAA ACATGTCAGG AGCAGCAATT TTAACTTCAA AGAACATTAA TGAGAATCCT 
GTAAGCCAAA ATTTGAAGAG CGCTTGTGAT GACAAATTCC AACTACAACA TCTGGAGGGT 
CCCACCAGTG GAGATGACGA GTCAGGGATG GGAAGAAGGA ACCCCATACC TGGGATCTGG 
AATCAGCCTT TTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169722.1
CDS	209..4606
Translation	MDLSAVQIQEVQNVLHAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNEITKRSLQGSTRFSQLAEELLRIMAAFELDTGMQLTNGFSFSKKRNNSCERLNEEASIIQSVGYRNRVRRLPQVEPGNATLKDSLGVQLSNLGIVRSVKKNRQTQPRKKSVYIELDSDSSEETVTKPGDCSVRDQELLQTAPQEAGDEGKLHSAEEAACEFSEGIRNIEHHQCSDDLNPTENHATERHPEKCQSISISNVCVEPCGTDAHASSLQPETSSLLLIEDRMNAEKAEFCNKSKQPGIAVSQQSRWAASKGTCNDRQVPSTGEKVGPNADSLSDREKWTHPQSLCPENSGATTDVPWITLNSSVQKVNEWFSRTGEMLTSDSASARRHESNAEAAVVLEVSNEVDGGFSSSRKTDLVTPDPHHTLMCKSGRDFSKPVEDNISDKIFGKSYQRKGSRPHLNHVTEIIGTFITEPQITQEQPFTNKLKRKRSTSLQPEDFIKKADSAGVQRTPDNINQGTDLMEPNEQAVSTTSNCQENKIAGSNLQKEKSAHPTESLRKEPASTAGAKSISNSVSDLEVELNVHSSKAPKKNRLRRKSSIRCALPLEPISRNPSPPTCAELQIDSCGSSEETKKNHSNQQPAGHLREPQLIEDTEPAADAKKNEPNEHIRKRRASDAFPEEKLMNKAGLLTSCSSPRKSQGPVNPSPQRTGTEQLETRQMSDSAKELGDRVLGGEPSGKTTDRSEESTSVSLVSDTDYDTQNSVSVLDAHTVRYARTGSAQCMTQFVASENPKELVHGSNNAGSGTEGLKPPLRHALNLSQEKVEMEDSELDTQYLQNTFQVSKRQSFALFSKPRSPQKDCAHSVPSKELSPKVTAKGKQKERQGQEEFEISHVQAVAATVGLPVPCQEGKLAADTMCDRGCRLCPSSHYRSGENGLSATGKSGISQNSHFKQSVSPIRSSIKTDNRKPLTEGRFERHTSSTEMAVGNENILQSTVHTVSLNNRGNACQEAGSGSIHEVCSTGDSFPGQLGRNRGPKVNTVPPLDSMQPGVCQQSVPVSDKYLEIKKQEGEAVCADFSPCLFSDHLEQSMSGKVFQVCSETPDDLLDDVEIQGHTSFGEGDIMERSAVFNGSILRRESSRSPSPVTHASKSQSLHRASRKLESSEESDSTEDEDLPCFQHLLSRISNTPELTRCSSAVTQRMPEKAEGTQAPWKGSSSDCNNEVIMIEASQEHQFSEDPRCSGSMFSSQHSAAQGSTANANSQDSNFIPPSKQRSHQCGNEEAFLSDKELISDNEEMATCLEEDNDQEEDSIIPDSEASGYESETNLSEDCSQSDILTTQQRATMKYNLIKLQQEMAHLEAVLEQRGNQPSGHSPSLLADPCALEDLPDLEPNMSGAAILTSKNINENPVSQNLKSACDDKFQLQHLEGPTSGDDESGMGRRNPIPGIWNQPFL

Target ORF information:

RefSeq Version	XM_017314233.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus breast cancer 1, early onset (Brca1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314233.1

ORF Insert Sequence:

ATGGATTTAT CTGCCGTCCA AATTCAAGAA GTACAAAATG TCCTTCATGC TATGCAGAAA 
ATCTTAGAGT GTCCGATCTG TTTGGAACTG ATCAAAGAAC CTGTTTCCAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTTAACC AGAAGAAAGG GCCTTCACAA 
TGTCCTTTGT GTAAGAATGA GATAACCAAA AGGAGCCTAC AGGGAAGCAC AAGGTTTAGT 
CAGCTTGCTG AAGAGCTGCT GAGAATAATG GCTGCTTTTG AGCTTGACAC GGGAATGCAG 
CTTACAAATG GTTTTAGTTT TTCAAAAAAG AGAAATAATT CTTGTGAGCG TTTGAATGAG 
GAGGCGTCGA TCATCCAGAG CGTGGGCTAC CGGAACCGTG TCAGAAGGCT TCCCCAGGTC 
GAACCTGGAA ATGCCACCTT GAAGGACAGC CTAGGTGTCC AGCTGTCTAA CCTTGGAATC 
GTGAGATCAG TGAAGAAAAA CAGGCAGACC CAACCTCGAA AGAAATCTGT CTACATTGAA 
CTAGACTCTG ATTCTTCTGA AGAGACAGTA ACTAAGCCAG GTGATTGCAG TGTGAGAGAC 
CAGGAATTGT TACAGACCGC CCCTCAAGAA GCTGGAGATG AAGGCAAGCT GCACTCTGCA 
GAAGAGGCTG CTTGTGAGTT TTCTGAGGGC ATAAGAAACA TTGAACATCA TCAATGCAGT 
GATGATTTAA ACCCTACTGA GAATCATGCA ACTGAAAGGC ATCCAGAAAA ATGTCAGAGT 
ATTTCTATTT CAAATGTGTG TGTGGAGCCA TGTGGCACAG ATGCTCATGC CAGCTCATTA 
CAGCCTGAGA CCAGCAGTTT ATTGCTCATT GAAGACAGAA TGAATGCAGA AAAGGCTGAA 
TTCTGTAATA AAAGCAAACA GCCTGGCATA GCAGTGAGCC AGCAGAGCAG ATGGGCTGCA 
AGTAAAGGAA CATGTAACGA CAGGCAGGTT CCCAGCACTG GGGAAAAGGT AGGTCCAAAC 
GCTGACTCCC TTAGTGATAG AGAGAAGTGG ACTCACCCGC AAAGTCTGTG CCCTGAGAAT 
TCTGGAGCTA CCACCGATGT TCCTTGGATA ACACTAAATA GCAGCGTTCA GAAAGTTAAT 
GAGTGGTTTT CCAGAACTGG TGAAATGTTA ACTTCTGACA GCGCATCTGC CAGGAGGCAC 
GAGTCAAATG CTGAAGCAGC TGTTGTGTTG GAAGTTTCAA ACGAAGTGGA TGGGGGTTTT 
AGTTCTTCAA GGAAAACAGA CTTAGTAACC CCCGACCCCC ATCATACTTT AATGTGTAAA 
AGTGGAAGAG ACTTCTCCAA ACCAGTAGAG GATAATATCA GTGATAAAAT ATTTGGGAAA 
TCCTATCAGA GAAAGGGAAG CCGCCCTCAC CTGAACCATG TGACTGAAAT TATAGGCACA 
TTTATTACAG AACCACAGAT AACACAAGAG CAGCCCTTCA CAAATAAATT AAAACGTAAG 
AGAAGTACAT CCCTTCAACC TGAGGACTTC ATCAAGAAAG CAGATTCAGC AGGTGTTCAA 
AGGACTCCTG ACAACATAAA TCAGGGAACT GACCTAATGG AGCCAAATGA GCAAGCAGTG 
AGTACTACCA GTAACTGTCA GGAGAACAAA ATAGCAGGTA GTAATCTCCA GAAAGAGAAA 
AGCGCTCATC CAACTGAATC ATTGAGAAAG GAACCTGCTT CCACAGCAGG AGCCAAATCT 
ATAAGCAACA GTGTAAGTGA TTTGGAGGTA GAATTAAACG TCCACAGTTC AAAAGCACCT 
AAGAAAAATA GGCTGAGGAG GAAGTCTTCT ATCAGGTGTG CTCTTCCACT TGAACCAATC 
AGTAGAAATC CAAGCCCACC TACTTGTGCT GAGCTTCAAA TCGATAGTTG TGGTAGCAGT 
GAAGAAACAA AGAAAAACCA TTCCAACCAA CAGCCAGCCG GGCACCTTAG AGAGCCTCAA 
CTCATCGAAG ACACTGAACC TGCAGCGGAT GCCAAGAAGA ACGAGCCAAA TGAACACATA 
AGGAAGAGAC GTGCCAGCGA TGCTTTCCCA GAAGAGAAAT TAATGAACAA AGCTGGTTTA 
TTAACTAGCT GTTCAAGTCC TAGAAAATCT CAAGGGCCTG TCAATCCCAG CCCTCAGAGA 
ACAGGAACAG AGCAACTTGA AACACGCCAA ATGTCTGACA GTGCCAAAGA ACTCGGGGAT 
CGGGTCCTAG GAGGAGAGCC CAGTGGCAAA ACCACTGACC GATCTGAGGA GAGCACCAGC 
GTATCCTTGG TATCTGACAC TGACTACGAC ACTCAGAACA GTGTCTCAGT CCTGGACGCT 
CACACTGTCA GATATGCAAG AACAGGATCC GCTCAGTGTA TGACTCAGTT TGTAGCAAGC 
GAAAACCCCA AGGAACTCGT CCATGGCTCT AACAATGCTG GGAGTGGCAC AGAGGGTCTC 
AAGCCCCCCT TGAGACACGC GCTTAACCTC AGTCAGGAGA AAGTAGAAAT GGAAGACAGT 
GAACTTGATA CTCAGTATTT GCAGAATACA TTTCAAGTTT CAAAGCGTCA GTCATTTGCT 
TTATTTTCAA AACCTAGAAG TCCCCAAAAG GACTGTGCTC ACTCTGTGCC CTCAAAGGAA 
CTGAGTCCAA AGGTGACAGC TAAAGGTAAA CAAAAAGAAC GTCAGGGACA GGAAGAATTT 
GAAATCAGTC ACGTACAAGC AGTTGCGGCC ACAGTGGGCT TACCTGTGCC CTGTCAAGAA 
GGTAAGCTAG CTGCTGATAC AATGTGTGAT AGAGGTTGTA GGCTTTGTCC ATCATCTCAT 
TACAGAAGCG GGGAGAATGG ACTCAGCGCC ACAGGTAAAT CAGGAATTTC ACAAAACTCA 
CATTTTAAAC AATCAGTTTC TCCCATCAGG TCATCTATAA AAACTGACAA TAGGAAACCT 
CTGACAGAGG GACGATTTGA GAGACATACA TCATCAACTG AGATGGCGGT GGGAAATGAG 
AACATTCTTC AGAGTACAGT GCACACAGTT AGCCTGAATA ACAGAGGAAA TGCTTGTCAA 
GAAGCCGGCT CGGGCAGTAT TCATGAAGTA TGTTCCACTG GTGACTCCTT CCCAGGACAA 
CTAGGTAGAA ACAGAGGGCC TAAGGTGAAC ACTGTGCCTC CATTAGATAG TATGCAGCCT 
GGTGTCTGTC AGCAAAGTGT TCCTGTAAGT GATAAGTATC TTGAAATAAA AAAGCAGGAG 
GGTGAGGCTG TCTGTGCAGA CTTCTCTCCA TGTCTATTCT CAGACCATCT TGAGCAATCT 
ATGAGTGGTA AGGTTTTTCA GGTTTGCTCT GAGACACCTG ATGACCTGCT GGATGATGTT 
GAAATACAGG GACATACTAG CTTTGGTGAA GGTGACATAA TGGAGAGATC TGCTGTCTTT 
AACGGAAGCA TCCTGAGAAG GGAGTCCAGT AGGAGCCCTA GTCCTGTAAC CCATGCATCG 
AAGTCTCAGA GTCTCCACAG AGCGTCTAGG AAATTAGAAT CGTCAGAAGA GAGCGACTCC 
ACTGAGGATG AAGATCTTCC CTGCTTCCAA CACTTACTGA GCAGAATAAG CAACACACCT 
GAGCTTACCA GATGCAGCAG TGCTGTGACA CAGCGTATGC CAGAGAAAGC GGAGGGGACC 
CAAGCACCAT GGAAGGGTAG CAGCAGTGAC TGCAATAATG AGGTGATCAT GATAGAGGCA 
TCTCAGGAGC ATCAGTTTAG TGAGGATCCA AGATGCTCTG GCAGCATGTT CTCTTCACAG 
CACAGTGCTG CCCAAGGGTC AACTGCAAAT GCAAACTCCC AGGATTCCAA TTTTATTCCA 
CCTTCCAAAC AGAGGAGTCA CCAGTGTGGG AATGAGGAAG CTTTCCTAAG TGACAAGGAA 
TTGATTTCAG ATAACGAGGA AATGGCAACT TGCCTAGAAG AGGATAATGA CCAAGAAGAG 
GATAGTATAA TCCCAGATTC AGAGGCATCC GGATACGAGA GTGAAACAAA CCTTTCTGAA 
GACTGCTCGC AGAGTGATAT TTTAACCACT CAGCAGCGGG CGACCATGAA GTATAACCTG 
ATAAAGCTGC AGCAGGAAAT GGCTCACCTG GAAGCTGTGC TGGAGCAGCG TGGGAACCAG 
CCTTCTGGCC ACTCCCCTTC CCTCCTAGCG GACCCTTGTG CCCTGGAAGA CCTGCCAGAT 
CTGGAACCAA ACATGTCAGG AGCAGCAATT TTAACTTCAA AGAACATTAA TGAGAATCCT 
GTAAGCCAAA ATTTGAAGAG CGCTTGTGAT GACAAATTCC AACTACAACA TCTGGAGGGT 
CCCACCAGTG GAGATGACGA GTCAGGGATG GGAAGAAGGA ACCCCATACC TGGGATCTGG 
AATCAGCCTT TTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brca1 ( XM_017314233.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169722.1 Mus musculus breast cancer 1, early onset (Brca1), transcript variant X3, mRNA.

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CloneID	OMu77714
Clone ID Related Accession (Same CDS sequence)	XM_017314233.1 , XM_017314233.2
Accession Version	XM_017314233.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	breast cancer type 1 susceptibility protein homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314233.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAT CTGCCGTCCA AATTCAAGAA GTACAAAATG TCCTTCATGC TATGCAGAAA 
ATCTTAGAGT GTCCGATCTG TTTGGAACTG ATCAAAGAAC CTGTTTCCAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTTAACC AGAAGAAAGG GCCTTCACAA 
TGTCCTTTGT GTAAGAATGA GATAACCAAA AGGAGCCTAC AGGGAAGCAC AAGGTTTAGT 
CAGCTTGCTG AAGAGCTGCT GAGAATAATG GCTGCTTTTG AGCTTGACAC GGGAATGCAG 
CTTACAAATG GTTTTAGTTT TTCAAAAAAG AGAAATAATT CTTGTGAGCG TTTGAATGAG 
GAGGCGTCGA TCATCCAGAG CGTGGGCTAC CGGAACCGTG TCAGAAGGCT TCCCCAGGTC 
GAACCTGGAA ATGCCACCTT GAAGGACAGC CTAGGTGTCC AGCTGTCTAA CCTTGGAATC 
GTGAGATCAG TGAAGAAAAA CAGGCAGACC CAACCTCGAA AGAAATCTGT CTACATTGAA 
CTAGACTCTG ATTCTTCTGA AGAGACAGTA ACTAAGCCAG GTGATTGCAG TGTGAGAGAC 
CAGGAATTGT TACAGACCGC CCCTCAAGAA GCTGGAGATG AAGGCAAGCT GCACTCTGCA 
GAAGAGGCTG CTTGTGAGTT TTCTGAGGGC ATAAGAAACA TTGAACATCA TCAATGCAGT 
GATGATTTAA ACCCTACTGA GAATCATGCA ACTGAAAGGC ATCCAGAAAA ATGTCAGAGT 
ATTTCTATTT CAAATGTGTG TGTGGAGCCA TGTGGCACAG ATGCTCATGC CAGCTCATTA 
CAGCCTGAGA CCAGCAGTTT ATTGCTCATT GAAGACAGAA TGAATGCAGA AAAGGCTGAA 
TTCTGTAATA AAAGCAAACA GCCTGGCATA GCAGTGAGCC AGCAGAGCAG ATGGGCTGCA 
AGTAAAGGAA CATGTAACGA CAGGCAGGTT CCCAGCACTG GGGAAAAGGT AGGTCCAAAC 
GCTGACTCCC TTAGTGATAG AGAGAAGTGG ACTCACCCGC AAAGTCTGTG CCCTGAGAAT 
TCTGGAGCTA CCACCGATGT TCCTTGGATA ACACTAAATA GCAGCGTTCA GAAAGTTAAT 
GAGTGGTTTT CCAGAACTGG TGAAATGTTA ACTTCTGACA GCGCATCTGC CAGGAGGCAC 
GAGTCAAATG CTGAAGCAGC TGTTGTGTTG GAAGTTTCAA ACGAAGTGGA TGGGGGTTTT 
AGTTCTTCAA GGAAAACAGA CTTAGTAACC CCCGACCCCC ATCATACTTT AATGTGTAAA 
AGTGGAAGAG ACTTCTCCAA ACCAGTAGAG GATAATATCA GTGATAAAAT ATTTGGGAAA 
TCCTATCAGA GAAAGGGAAG CCGCCCTCAC CTGAACCATG TGACTGAAAT TATAGGCACA 
TTTATTACAG AACCACAGAT AACACAAGAG CAGCCCTTCA CAAATAAATT AAAACGTAAG 
AGAAGTACAT CCCTTCAACC TGAGGACTTC ATCAAGAAAG CAGATTCAGC AGGTGTTCAA 
AGGACTCCTG ACAACATAAA TCAGGGAACT GACCTAATGG AGCCAAATGA GCAAGCAGTG 
AGTACTACCA GTAACTGTCA GGAGAACAAA ATAGCAGGTA GTAATCTCCA GAAAGAGAAA 
AGCGCTCATC CAACTGAATC ATTGAGAAAG GAACCTGCTT CCACAGCAGG AGCCAAATCT 
ATAAGCAACA GTGTAAGTGA TTTGGAGGTA GAATTAAACG TCCACAGTTC AAAAGCACCT 
AAGAAAAATA GGCTGAGGAG GAAGTCTTCT ATCAGGTGTG CTCTTCCACT TGAACCAATC 
AGTAGAAATC CAAGCCCACC TACTTGTGCT GAGCTTCAAA TCGATAGTTG TGGTAGCAGT 
GAAGAAACAA AGAAAAACCA TTCCAACCAA CAGCCAGCCG GGCACCTTAG AGAGCCTCAA 
CTCATCGAAG ACACTGAACC TGCAGCGGAT GCCAAGAAGA ACGAGCCAAA TGAACACATA 
AGGAAGAGAC GTGCCAGCGA TGCTTTCCCA GAAGAGAAAT TAATGAACAA AGCTGGTTTA 
TTAACTAGCT GTTCAAGTCC TAGAAAATCT CAAGGGCCTG TCAATCCCAG CCCTCAGAGA 
ACAGGAACAG AGCAACTTGA AACACGCCAA ATGTCTGACA GTGCCAAAGA ACTCGGGGAT 
CGGGTCCTAG GAGGAGAGCC CAGTGGCAAA ACCACTGACC GATCTGAGGA GAGCACCAGC 
GTATCCTTGG TATCTGACAC TGACTACGAC ACTCAGAACA GTGTCTCAGT CCTGGACGCT 
CACACTGTCA GATATGCAAG AACAGGATCC GCTCAGTGTA TGACTCAGTT TGTAGCAAGC 
GAAAACCCCA AGGAACTCGT CCATGGCTCT AACAATGCTG GGAGTGGCAC AGAGGGTCTC 
AAGCCCCCCT TGAGACACGC GCTTAACCTC AGTCAGGAGA AAGTAGAAAT GGAAGACAGT 
GAACTTGATA CTCAGTATTT GCAGAATACA TTTCAAGTTT CAAAGCGTCA GTCATTTGCT 
TTATTTTCAA AACCTAGAAG TCCCCAAAAG GACTGTGCTC ACTCTGTGCC CTCAAAGGAA 
CTGAGTCCAA AGGTGACAGC TAAAGGTAAA CAAAAAGAAC GTCAGGGACA GGAAGAATTT 
GAAATCAGTC ACGTACAAGC AGTTGCGGCC ACAGTGGGCT TACCTGTGCC CTGTCAAGAA 
GGTAAGCTAG CTGCTGATAC AATGTGTGAT AGAGGTTGTA GGCTTTGTCC ATCATCTCAT 
TACAGAAGCG GGGAGAATGG ACTCAGCGCC ACAGGTAAAT CAGGAATTTC ACAAAACTCA 
CATTTTAAAC AATCAGTTTC TCCCATCAGG TCATCTATAA AAACTGACAA TAGGAAACCT 
CTGACAGAGG GACGATTTGA GAGACATACA TCATCAACTG AGATGGCGGT GGGAAATGAG 
AACATTCTTC AGAGTACAGT GCACACAGTT AGCCTGAATA ACAGAGGAAA TGCTTGTCAA 
GAAGCCGGCT CGGGCAGTAT TCATGAAGTA TGTTCCACTG GTGACTCCTT CCCAGGACAA 
CTAGGTAGAA ACAGAGGGCC TAAGGTGAAC ACTGTGCCTC CATTAGATAG TATGCAGCCT 
GGTGTCTGTC AGCAAAGTGT TCCTGTAAGT GATAAGTATC TTGAAATAAA AAAGCAGGAG 
GGTGAGGCTG TCTGTGCAGA CTTCTCTCCA TGTCTATTCT CAGACCATCT TGAGCAATCT 
ATGAGTGGTA AGGTTTTTCA GGTTTGCTCT GAGACACCTG ATGACCTGCT GGATGATGTT 
GAAATACAGG GACATACTAG CTTTGGTGAA GGTGACATAA TGGAGAGATC TGCTGTCTTT 
AACGGAAGCA TCCTGAGAAG GGAGTCCAGT AGGAGCCCTA GTCCTGTAAC CCATGCATCG 
AAGTCTCAGA GTCTCCACAG AGCGTCTAGG AAATTAGAAT CGTCAGAAGA GAGCGACTCC 
ACTGAGGATG AAGATCTTCC CTGCTTCCAA CACTTACTGA GCAGAATAAG CAACACACCT 
GAGCTTACCA GATGCAGCAG TGCTGTGACA CAGCGTATGC CAGAGAAAGC GGAGGGGACC 
CAAGCACCAT GGAAGGGTAG CAGCAGTGAC TGCAATAATG AGGTGATCAT GATAGAGGCA 
TCTCAGGAGC ATCAGTTTAG TGAGGATCCA AGATGCTCTG GCAGCATGTT CTCTTCACAG 
CACAGTGCTG CCCAAGGGTC AACTGCAAAT GCAAACTCCC AGGATTCCAA TTTTATTCCA 
CCTTCCAAAC AGAGGAGTCA CCAGTGTGGG AATGAGGAAG CTTTCCTAAG TGACAAGGAA 
TTGATTTCAG ATAACGAGGA AATGGCAACT TGCCTAGAAG AGGATAATGA CCAAGAAGAG 
GATAGTATAA TCCCAGATTC AGAGGCATCC GGATACGAGA GTGAAACAAA CCTTTCTGAA 
GACTGCTCGC AGAGTGATAT TTTAACCACT CAGCAGCGGG CGACCATGAA GTATAACCTG 
ATAAAGCTGC AGCAGGAAAT GGCTCACCTG GAAGCTGTGC TGGAGCAGCG TGGGAACCAG 
CCTTCTGGCC ACTCCCCTTC CCTCCTAGCG GACCCTTGTG CCCTGGAAGA CCTGCCAGAT 
CTGGAACCAA ACATGTCAGG AGCAGCAATT TTAACTTCAA AGAACATTAA TGAGAATCCT 
GTAAGCCAAA ATTTGAAGAG CGCTTGTGAT GACAAATTCC AACTACAACA TCTGGAGGGT 
CCCACCAGTG GAGATGACGA GTCAGGGATG GGAAGAAGGA ACCCCATACC TGGGATCTGG 
AATCAGCCTT TTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169722.1
CDS	206..4603
Translation	MDLSAVQIQEVQNVLHAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNEITKRSLQGSTRFSQLAEELLRIMAAFELDTGMQLTNGFSFSKKRNNSCERLNEEASIIQSVGYRNRVRRLPQVEPGNATLKDSLGVQLSNLGIVRSVKKNRQTQPRKKSVYIELDSDSSEETVTKPGDCSVRDQELLQTAPQEAGDEGKLHSAEEAACEFSEGIRNIEHHQCSDDLNPTENHATERHPEKCQSISISNVCVEPCGTDAHASSLQPETSSLLLIEDRMNAEKAEFCNKSKQPGIAVSQQSRWAASKGTCNDRQVPSTGEKVGPNADSLSDREKWTHPQSLCPENSGATTDVPWITLNSSVQKVNEWFSRTGEMLTSDSASARRHESNAEAAVVLEVSNEVDGGFSSSRKTDLVTPDPHHTLMCKSGRDFSKPVEDNISDKIFGKSYQRKGSRPHLNHVTEIIGTFITEPQITQEQPFTNKLKRKRSTSLQPEDFIKKADSAGVQRTPDNINQGTDLMEPNEQAVSTTSNCQENKIAGSNLQKEKSAHPTESLRKEPASTAGAKSISNSVSDLEVELNVHSSKAPKKNRLRRKSSIRCALPLEPISRNPSPPTCAELQIDSCGSSEETKKNHSNQQPAGHLREPQLIEDTEPAADAKKNEPNEHIRKRRASDAFPEEKLMNKAGLLTSCSSPRKSQGPVNPSPQRTGTEQLETRQMSDSAKELGDRVLGGEPSGKTTDRSEESTSVSLVSDTDYDTQNSVSVLDAHTVRYARTGSAQCMTQFVASENPKELVHGSNNAGSGTEGLKPPLRHALNLSQEKVEMEDSELDTQYLQNTFQVSKRQSFALFSKPRSPQKDCAHSVPSKELSPKVTAKGKQKERQGQEEFEISHVQAVAATVGLPVPCQEGKLAADTMCDRGCRLCPSSHYRSGENGLSATGKSGISQNSHFKQSVSPIRSSIKTDNRKPLTEGRFERHTSSTEMAVGNENILQSTVHTVSLNNRGNACQEAGSGSIHEVCSTGDSFPGQLGRNRGPKVNTVPPLDSMQPGVCQQSVPVSDKYLEIKKQEGEAVCADFSPCLFSDHLEQSMSGKVFQVCSETPDDLLDDVEIQGHTSFGEGDIMERSAVFNGSILRRESSRSPSPVTHASKSQSLHRASRKLESSEESDSTEDEDLPCFQHLLSRISNTPELTRCSSAVTQRMPEKAEGTQAPWKGSSSDCNNEVIMIEASQEHQFSEDPRCSGSMFSSQHSAAQGSTANANSQDSNFIPPSKQRSHQCGNEEAFLSDKELISDNEEMATCLEEDNDQEEDSIIPDSEASGYESETNLSEDCSQSDILTTQQRATMKYNLIKLQQEMAHLEAVLEQRGNQPSGHSPSLLADPCALEDLPDLEPNMSGAAILTSKNINENPVSQNLKSACDDKFQLQHLEGPTSGDDESGMGRRNPIPGIWNQPFL

Target ORF information:

RefSeq Version	XM_017314233.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus breast cancer 1, early onset (Brca1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314233.2

ORF Insert Sequence:

ATGGATTTAT CTGCCGTCCA AATTCAAGAA GTACAAAATG TCCTTCATGC TATGCAGAAA 
ATCTTAGAGT GTCCGATCTG TTTGGAACTG ATCAAAGAAC CTGTTTCCAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTTAACC AGAAGAAAGG GCCTTCACAA 
TGTCCTTTGT GTAAGAATGA GATAACCAAA AGGAGCCTAC AGGGAAGCAC AAGGTTTAGT 
CAGCTTGCTG AAGAGCTGCT GAGAATAATG GCTGCTTTTG AGCTTGACAC GGGAATGCAG 
CTTACAAATG GTTTTAGTTT TTCAAAAAAG AGAAATAATT CTTGTGAGCG TTTGAATGAG 
GAGGCGTCGA TCATCCAGAG CGTGGGCTAC CGGAACCGTG TCAGAAGGCT TCCCCAGGTC 
GAACCTGGAA ATGCCACCTT GAAGGACAGC CTAGGTGTCC AGCTGTCTAA CCTTGGAATC 
GTGAGATCAG TGAAGAAAAA CAGGCAGACC CAACCTCGAA AGAAATCTGT CTACATTGAA 
CTAGACTCTG ATTCTTCTGA AGAGACAGTA ACTAAGCCAG GTGATTGCAG TGTGAGAGAC 
CAGGAATTGT TACAGACCGC CCCTCAAGAA GCTGGAGATG AAGGCAAGCT GCACTCTGCA 
GAAGAGGCTG CTTGTGAGTT TTCTGAGGGC ATAAGAAACA TTGAACATCA TCAATGCAGT 
GATGATTTAA ACCCTACTGA GAATCATGCA ACTGAAAGGC ATCCAGAAAA ATGTCAGAGT 
ATTTCTATTT CAAATGTGTG TGTGGAGCCA TGTGGCACAG ATGCTCATGC CAGCTCATTA 
CAGCCTGAGA CCAGCAGTTT ATTGCTCATT GAAGACAGAA TGAATGCAGA AAAGGCTGAA 
TTCTGTAATA AAAGCAAACA GCCTGGCATA GCAGTGAGCC AGCAGAGCAG ATGGGCTGCA 
AGTAAAGGAA CATGTAACGA CAGGCAGGTT CCCAGCACTG GGGAAAAGGT AGGTCCAAAC 
GCTGACTCCC TTAGTGATAG AGAGAAGTGG ACTCACCCGC AAAGTCTGTG CCCTGAGAAT 
TCTGGAGCTA CCACCGATGT TCCTTGGATA ACACTAAATA GCAGCGTTCA GAAAGTTAAT 
GAGTGGTTTT CCAGAACTGG TGAAATGTTA ACTTCTGACA GCGCATCTGC CAGGAGGCAC 
GAGTCAAATG CTGAAGCAGC TGTTGTGTTG GAAGTTTCAA ACGAAGTGGA TGGGGGTTTT 
AGTTCTTCAA GGAAAACAGA CTTAGTAACC CCCGACCCCC ATCATACTTT AATGTGTAAA 
AGTGGAAGAG ACTTCTCCAA ACCAGTAGAG GATAATATCA GTGATAAAAT ATTTGGGAAA 
TCCTATCAGA GAAAGGGAAG CCGCCCTCAC CTGAACCATG TGACTGAAAT TATAGGCACA 
TTTATTACAG AACCACAGAT AACACAAGAG CAGCCCTTCA CAAATAAATT AAAACGTAAG 
AGAAGTACAT CCCTTCAACC TGAGGACTTC ATCAAGAAAG CAGATTCAGC AGGTGTTCAA 
AGGACTCCTG ACAACATAAA TCAGGGAACT GACCTAATGG AGCCAAATGA GCAAGCAGTG 
AGTACTACCA GTAACTGTCA GGAGAACAAA ATAGCAGGTA GTAATCTCCA GAAAGAGAAA 
AGCGCTCATC CAACTGAATC ATTGAGAAAG GAACCTGCTT CCACAGCAGG AGCCAAATCT 
ATAAGCAACA GTGTAAGTGA TTTGGAGGTA GAATTAAACG TCCACAGTTC AAAAGCACCT 
AAGAAAAATA GGCTGAGGAG GAAGTCTTCT ATCAGGTGTG CTCTTCCACT TGAACCAATC 
AGTAGAAATC CAAGCCCACC TACTTGTGCT GAGCTTCAAA TCGATAGTTG TGGTAGCAGT 
GAAGAAACAA AGAAAAACCA TTCCAACCAA CAGCCAGCCG GGCACCTTAG AGAGCCTCAA 
CTCATCGAAG ACACTGAACC TGCAGCGGAT GCCAAGAAGA ACGAGCCAAA TGAACACATA 
AGGAAGAGAC GTGCCAGCGA TGCTTTCCCA GAAGAGAAAT TAATGAACAA AGCTGGTTTA 
TTAACTAGCT GTTCAAGTCC TAGAAAATCT CAAGGGCCTG TCAATCCCAG CCCTCAGAGA 
ACAGGAACAG AGCAACTTGA AACACGCCAA ATGTCTGACA GTGCCAAAGA ACTCGGGGAT 
CGGGTCCTAG GAGGAGAGCC CAGTGGCAAA ACCACTGACC GATCTGAGGA GAGCACCAGC 
GTATCCTTGG TATCTGACAC TGACTACGAC ACTCAGAACA GTGTCTCAGT CCTGGACGCT 
CACACTGTCA GATATGCAAG AACAGGATCC GCTCAGTGTA TGACTCAGTT TGTAGCAAGC 
GAAAACCCCA AGGAACTCGT CCATGGCTCT AACAATGCTG GGAGTGGCAC AGAGGGTCTC 
AAGCCCCCCT TGAGACACGC GCTTAACCTC AGTCAGGAGA AAGTAGAAAT GGAAGACAGT 
GAACTTGATA CTCAGTATTT GCAGAATACA TTTCAAGTTT CAAAGCGTCA GTCATTTGCT 
TTATTTTCAA AACCTAGAAG TCCCCAAAAG GACTGTGCTC ACTCTGTGCC CTCAAAGGAA 
CTGAGTCCAA AGGTGACAGC TAAAGGTAAA CAAAAAGAAC GTCAGGGACA GGAAGAATTT 
GAAATCAGTC ACGTACAAGC AGTTGCGGCC ACAGTGGGCT TACCTGTGCC CTGTCAAGAA 
GGTAAGCTAG CTGCTGATAC AATGTGTGAT AGAGGTTGTA GGCTTTGTCC ATCATCTCAT 
TACAGAAGCG GGGAGAATGG ACTCAGCGCC ACAGGTAAAT CAGGAATTTC ACAAAACTCA 
CATTTTAAAC AATCAGTTTC TCCCATCAGG TCATCTATAA AAACTGACAA TAGGAAACCT 
CTGACAGAGG GACGATTTGA GAGACATACA TCATCAACTG AGATGGCGGT GGGAAATGAG 
AACATTCTTC AGAGTACAGT GCACACAGTT AGCCTGAATA ACAGAGGAAA TGCTTGTCAA 
GAAGCCGGCT CGGGCAGTAT TCATGAAGTA TGTTCCACTG GTGACTCCTT CCCAGGACAA 
CTAGGTAGAA ACAGAGGGCC TAAGGTGAAC ACTGTGCCTC CATTAGATAG TATGCAGCCT 
GGTGTCTGTC AGCAAAGTGT TCCTGTAAGT GATAAGTATC TTGAAATAAA AAAGCAGGAG 
GGTGAGGCTG TCTGTGCAGA CTTCTCTCCA TGTCTATTCT CAGACCATCT TGAGCAATCT 
ATGAGTGGTA AGGTTTTTCA GGTTTGCTCT GAGACACCTG ATGACCTGCT GGATGATGTT 
GAAATACAGG GACATACTAG CTTTGGTGAA GGTGACATAA TGGAGAGATC TGCTGTCTTT 
AACGGAAGCA TCCTGAGAAG GGAGTCCAGT AGGAGCCCTA GTCCTGTAAC CCATGCATCG 
AAGTCTCAGA GTCTCCACAG AGCGTCTAGG AAATTAGAAT CGTCAGAAGA GAGCGACTCC 
ACTGAGGATG AAGATCTTCC CTGCTTCCAA CACTTACTGA GCAGAATAAG CAACACACCT 
GAGCTTACCA GATGCAGCAG TGCTGTGACA CAGCGTATGC CAGAGAAAGC GGAGGGGACC 
CAAGCACCAT GGAAGGGTAG CAGCAGTGAC TGCAATAATG AGGTGATCAT GATAGAGGCA 
TCTCAGGAGC ATCAGTTTAG TGAGGATCCA AGATGCTCTG GCAGCATGTT CTCTTCACAG 
CACAGTGCTG CCCAAGGGTC AACTGCAAAT GCAAACTCCC AGGATTCCAA TTTTATTCCA 
CCTTCCAAAC AGAGGAGTCA CCAGTGTGGG AATGAGGAAG CTTTCCTAAG TGACAAGGAA 
TTGATTTCAG ATAACGAGGA AATGGCAACT TGCCTAGAAG AGGATAATGA CCAAGAAGAG 
GATAGTATAA TCCCAGATTC AGAGGCATCC GGATACGAGA GTGAAACAAA CCTTTCTGAA 
GACTGCTCGC AGAGTGATAT TTTAACCACT CAGCAGCGGG CGACCATGAA GTATAACCTG 
ATAAAGCTGC AGCAGGAAAT GGCTCACCTG GAAGCTGTGC TGGAGCAGCG TGGGAACCAG 
CCTTCTGGCC ACTCCCCTTC CCTCCTAGCG GACCCTTGTG CCCTGGAAGA CCTGCCAGAT 
CTGGAACCAA ACATGTCAGG AGCAGCAATT TTAACTTCAA AGAACATTAA TGAGAATCCT 
GTAAGCCAAA ATTTGAAGAG CGCTTGTGAT GACAAATTCC AACTACAACA TCTGGAGGGT 
CCCACCAGTG GAGATGACGA GTCAGGGATG GGAAGAAGGA ACCCCATACC TGGGATCTGG 
AATCAGCCTT TTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brca1 ( XM_030245495.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101355.1 Mus musculus breast cancer 1, early onset (Brca1), transcript variant X1, mRNA.

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CloneID	OMu20079
Clone ID Related Accession (Same CDS sequence)	XM_030245495.1 , NM_009764.3
Accession Version	XM_030245495.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	breast cancer type 1 susceptibility protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAT CTGCCGTCCA AATTCAAGAA GTACAAAATG TCCTTCATGC TATGCAGAAA 
ATCTTAGAGT GTCCGATCTG TTTGGAACTG ATCAAAGAAC CTGTTTCCAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTTAACC AGAAGAAAGG GCCTTCACAA 
TGTCCTTTGT GTAAGAATGA GATAACCAAA AGGAGCCTAC AGGGAAGCAC AAGGTTTAGT 
CAGCTTGCTG AAGAGCTGCT GAGAATAATG GCTGCTTTTG AGCTTGACAC GGGAATGCAG 
CTTACAAATG GTTTTAGTTT TTCAAAAAAG AGAAATAATT CTTGTGAGCG TTTGAATGAG 
GAGGCGTCGA TCATCCAGAG CGTGGGCTAC CGGAACCGTG TCAGAAGGCT TCCCCAGGTC 
GAACCTGGAA ATGCCACCTT GAAGGACAGC CTAGGTGTCC AGCTGTCTAA CCTTGGAATC 
GTGAGATCAG TGAAGAAAAA CAGGCAGACC CAACCTCGAA AGAAATCTGT CTACATTGAA 
CTAGACTCTG ATTCTTCTGA AGAGACAGTA ACTAAGCCAG GTGATTGCAG TGTGAGAGAC 
CAGGAATTGT TACAGACCGC CCCTCAAGAA GCTGGAGATG AAGGCAAGCT GCACTCTGCA 
GAAGAGGCTG CTTGTGAGTT TTCTGAGGGC ATAAGAAACA TTGAACATCA TCAATGCAGT 
GATGATTTAA ACCCTACTGA GAATCATGCA ACTGAAAGGC ATCCAGAAAA ATGTCAGAGT 
ATTTCTATTT CAAATGTGTG TGTGGAGCCA TGTGGCACAG ATGCTCATGC CAGCTCATTA 
CAGCCTGAGA CCAGCAGTTT ATTGCTCATT GAAGACAGAA TGAATGCAGA AAAGGCTGAA 
TTCTGTAATA AAAGCAAACA GCCTGGCATA GCAGTGAGCC AGCAGAGCAG ATGGGCTGCA 
AGTAAAGGAA CATGTAACGA CAGGCAGGTT CCCAGCACTG GGGAAAAGGT AGGTCCAAAC 
GCTGACTCCC TTAGTGATAG AGAGAAGTGG ACTCACCCGC AAAGTCTGTG CCCTGAGAAT 
TCTGGAGCTA CCACCGATGT TCCTTGGATA ACACTAAATA GCAGCGTTCA GAAAGTTAAT 
GAGTGGTTTT CCAGAACTGG TGAAATGTTA ACTTCTGACA GCGCATCTGC CAGGAGGCAC 
GAGTCAAATG CTGAAGCAGC TGTTGTGTTG GAAGTTTCAA ACGAAGTGGA TGGGGGTTTT 
AGTTCTTCAA GGAAAACAGA CTTAGTAACC CCCGACCCCC ATCATACTTT AATGTGTAAA 
AGTGGAAGAG ACTTCTCCAA ACCAGTAGAG GATAATATCA GTGATAAAAT ATTTGGGAAA 
TCCTATCAGA GAAAGGGAAG CCGCCCTCAC CTGAACCATG TGACTGAAAT TATAGGCACA 
TTTATTACAG AACCACAGAT AACACAAGAG CAGCCCTTCA CAAATAAATT AAAACGTAAG 
AGAAGTACAT CCCTTCAACC TGAGGACTTC ATCAAGAAAG CAGATTCAGC AGGTGTTCAA 
AGGACTCCTG ACAACATAAA TCAGGGAACT GACCTAATGG AGCCAAATGA GCAAGCAGTG 
AGTACTACCA GTAACTGTCA GGAGAACAAA ATAGCAGGTA GTAATCTCCA GAAAGAGAAA 
AGCGCTCATC CAACTGAATC ATTGAGAAAG GAACCTGCTT CCACAGCAGG AGCCAAATCT 
ATAAGCAACA GTGTAAGTGA TTTGGAGGTA GAATTAAACG TCCACAGTTC AAAAGCACCT 
AAGAAAAATA GGCTGAGGAG GAAGTCTTCT ATCAGGTGTG CTCTTCCACT TGAACCAATC 
AGTAGAAATC CAAGCCCACC TACTTGTGCT GAGCTTCAAA TCGATAGTTG TGGTAGCAGT 
GAAGAAACAA AGAAAAACCA TTCCAACCAA CAGCCAGCCG GGCACCTTAG AGAGCCTCAA 
CTCATCGAAG ACACTGAACC TGCAGCGGAT GCCAAGAAGA ACGAGCCAAA TGAACACATA 
AGGAAGAGAC GTGCCAGCGA TGCTTTCCCA GAAGAGAAAT TAATGAACAA AGCTGGTTTA 
TTAACTAGCT GTTCAAGTCC TAGAAAATCT CAAGGGCCTG TCAATCCCAG CCCTCAGAGA 
ACAGGAACAG AGCAACTTGA AACACGCCAA ATGTCTGACA GTGCCAAAGA ACTCGGGGAT 
CGGGTCCTAG GAGGAGAGCC CAGTGGCAAA ACCACTGACC GATCTGAGGA GAGCACCAGC 
GTATCCTTGG TATCTGACAC TGACTACGAC ACTCAGAACA GTGTCTCAGT CCTGGACGCT 
CACACTGTCA GATATGCAAG AACAGGATCC GCTCAGTGTA TGACTCAGTT TGTAGCAAGC 
GAAAACCCCA AGGAACTCGT CCATGGCTCT AACAATGCTG GGAGTGGCAC AGAGGGTCTC 
AAGCCCCCCT TGAGACACGC GCTTAACCTC AGTCAGGAGA AAGTAGAAAT GGAAGACAGT 
GAACTTGATA CTCAGTATTT GCAGAATACA TTTCAAGTTT CAAAGCGTCA GTCATTTGCT 
TTATTTTCAA AACCTAGAAG TCCCCAAAAG GACTGTGCTC ACTCTGTGCC CTCAAAGGAA 
CTGAGTCCAA AGGTGACAGC TAAAGGTAAA CAAAAAGAAC GTCAGGGACA GGAAGAATTT 
GAAATCAGTC ACGTACAAGC AGTTGCGGCC ACAGTGGGCT TACCTGTGCC CTGTCAAGAA 
GGTAAGCTAG CTGCTGATAC AATGTGTGAT AGAGGTTGTA GGCTTTGTCC ATCATCTCAT 
TACAGAAGCG GGGAGAATGG ACTCAGCGCC ACAGGTAAAT CAGGAATTTC ACAAAACTCA 
CATTTTAAAC AATCAGTTTC TCCCATCAGG TCATCTATAA AAACTGACAA TAGGAAACCT 
CTGACAGAGG GACGATTTGA GAGACATACA TCATCAACTG AGATGGCGGT GGGAAATGAG 
AACATTCTTC AGAGTACAGT GCACACAGTT AGCCTGAATA ACAGAGGAAA TGCTTGTCAA 
GAAGCCGGCT CGGGCAGTAT TCATGAAGTA TGTTCCACTG GTGACTCCTT CCCAGGACAA 
CTAGGTAGAA ACAGAGGGCC TAAGGTGAAC ACTGTGCCTC CATTAGATAG TATGCAGCCT 
GGTGTCTGTC AGCAAAGTGT TCCTGTAAGT GATAAGTATC TTGAAATAAA AAAGCAGGAG 
GGTGAGGCTG TCTGTGCAGA CTTCTCTCCA TGTCTATTCT CAGACCATCT TGAGCAATCT 
ATGAGTGGTA AGGTTTTTCA GGTTTGCTCT GAGACACCTG ATGACCTGCT GGATGATGTT 
GAAATACAGG GACATACTAG CTTTGGTGAA GGTGACATAA TGGAGAGATC TGCTGTCTTT 
AACGGAAGCA TCCTGAGAAG GGAGTCCAGT AGGAGCCCTA GTCCTGTAAC CCATGCATCG 
AAGTCTCAGA GTCTCCACAG AGCGTCTAGG AAATTAGAAT CGTCAGAAGA GAGCGACTCC 
ACTGAGGATG AAGATCTTCC CTGCTTCCAA CACTTACTGA GCAGAATAAG CAACACACCT 
GAGCTTACCA GATGCAGCAG TGCTGTGACA CAGCGTATGC CAGAGAAAGC GGAGGGGACC 
CAAGCACCAT GGAAGGGTAG CAGCAGTGAC TGCAATAATG AGGTGATCAT GATAGAGGCA 
TCTCAGGAGC ATCAGTTTAG TGAGGATCCA AGATGCTCTG GCAGCATGTT CTCTTCACAG 
CACAGTGCTG CCCAAGGGTC AACTGCAAAT GCAAACTCCC AGGATTCCAA TTTTATTCCA 
CCTTCCAAAC AGAGGAGTCA CCAGTGTGGG AATGAGGAAG CTTTCCTAAG TGACAAGGAA 
TTGATTTCAG ATAACGAGGA AATGGCAACT TGCCTAGAAG AGGATAATGA CCAAGAAGAG 
GATAGTATAA TCCCAGATTC AGAGGCATCC GGATACGAGA GTGAAACAAA CCTTTCTGAA 
GACTGCTCGC AGAGTGATAT TTTAACCACT CAGCAGCGGG CGACCATGAA GTATAACCTG 
ATAAAGCTGC AGCAGGAAAT GGCTCACCTG GAAGCTGTGC TGGAGCAGCG TGGGAACCAG 
CCTTCTGGCC ACTCCCCTTC CCTCCTAGCG GACCCTTGTG CCCTGGAAGA CCTGCCAGAT 
CTGGAACCAA ACATGTCAGG AGCAGCAATT TTAACTTCAA AGAACATTAA TGAGAATCCT 
GTAAGCCAAA ATTTGAAGAG CGCTTGTGAT GACAAATTCC AACTACAACA TCTGGAGGGT 
CCCACCAGTG GAGATGACGA GTCAGGGATG GGAAGGCCTT CCCCTTTTAA ATCTCCGTTG 
GCAGGCAGTA GGGGCTCTGC ACATGGCTGC TCTAGGCATC TTCAAAAGAG AAACTCCCCC 
TCTCAGGAGG AGCTCCTCCA GCCTGCTGGA TCAGAGGCGT CATCTGAGCC ACACAATTCA 
ACAGGGCAGT CTTGCCTGCC AAGGCGAGAG CTAGAAGGAA CCCCATACCT GGGATCTGGA 
ATCAGCCTTT TCTCTAGTAG AGACCCCGAA TCTGAGTCCC CTAAAGAGCC AGCCCACATT 
GGCACCACAC CAGCTTCAAC CTCTGCACTG AAAATACCCC AAGGTCAAGT TGCTTTCCGG 
AGTGCAGCTG CTGCTGGTGC TGATAAAGCA GTGGTAGGAA TTGTGAGCAA GATAAAGCCG 
GAATTGACAT CTTCAGAAGA AAGAGCGGAT AGAGACATAT CCATGGTGGT GTCAGGCTTG 
ACCCCCAAAG AAGTAATGAC CGTGCAAAAG TTTGCTGAAA AATACCGCCT CACTTTAACT 
GACGCAATTA CTGAGGAGAC TACACATGTA ATTATAAAAA CAGATGCGGA GTTTGTGTGT 
GAGCGGACAC TGAAATATTT TCTGGGCATT GCAGGAGGAA AGTGGATAGT TAGCTATTCA 
TGGGTGGTCC GGTCTATCCA AGAAAGAAGA CTTCTGAATG TGCATGAATT TGAAGTCAAA 
GGAGATGTTG TGACTGGAAG AAATCACCAA GGTCCAAGGC GATCCAGAGA ATCCCGGGAA 
AAGCTCTTCA AGGGCCTACA GGTCTATTGT TGTGAGCCCT TCACCAACAT GCCCAAAGAT 
GAGCTGGAGA GGATGCTGCA GCTGTGTGGG GCTTCCGTGG TGAAGGAGCT TCCATCGCTC 
ACCCATGACA CAGGTGCTCA TCTAGTTGTG ATCGTGCAGC CAAGCGCCTG GACAGAAGAC 
AGCAACTGCC CAGATATTGG GCAGCTGTGC AAGGCACGTC TTGTGATGTG GGACTGGGTG 
TTGGACAGTC TATCCAGCTA CCGGTGTCGG GATCTGGATG CCTACCTGGT ACAGAATATC 
ACCTGTGACA GTAGTGAGCC ACAAGACTCC AATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101355.1
CDS	340..5778
Translation	MDLSAVQIQEVQNVLHAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNEITKRSLQGSTRFSQLAEELLRIMAAFELDTGMQLTNGFSFSKKRNNSCERLNEEASIIQSVGYRNRVRRLPQVEPGNATLKDSLGVQLSNLGIVRSVKKNRQTQPRKKSVYIELDSDSSEETVTKPGDCSVRDQELLQTAPQEAGDEGKLHSAEEAACEFSEGIRNIEHHQCSDDLNPTENHATERHPEKCQSISISNVCVEPCGTDAHASSLQPETSSLLLIEDRMNAEKAEFCNKSKQPGIAVSQQSRWAASKGTCNDRQVPSTGEKVGPNADSLSDREKWTHPQSLCPENSGATTDVPWITLNSSVQKVNEWFSRTGEMLTSDSASARRHESNAEAAVVLEVSNEVDGGFSSSRKTDLVTPDPHHTLMCKSGRDFSKPVEDNISDKIFGKSYQRKGSRPHLNHVTEIIGTFITEPQITQEQPFTNKLKRKRSTSLQPEDFIKKADSAGVQRTPDNINQGTDLMEPNEQAVSTTSNCQENKIAGSNLQKEKSAHPTESLRKEPASTAGAKSISNSVSDLEVELNVHSSKAPKKNRLRRKSSIRCALPLEPISRNPSPPTCAELQIDSCGSSEETKKNHSNQQPAGHLREPQLIEDTEPAADAKKNEPNEHIRKRRASDAFPEEKLMNKAGLLTSCSSPRKSQGPVNPSPQRTGTEQLETRQMSDSAKELGDRVLGGEPSGKTTDRSEESTSVSLVSDTDYDTQNSVSVLDAHTVRYARTGSAQCMTQFVASENPKELVHGSNNAGSGTEGLKPPLRHALNLSQEKVEMEDSELDTQYLQNTFQVSKRQSFALFSKPRSPQKDCAHSVPSKELSPKVTAKGKQKERQGQEEFEISHVQAVAATVGLPVPCQEGKLAADTMCDRGCRLCPSSHYRSGENGLSATGKSGISQNSHFKQSVSPIRSSIKTDNRKPLTEGRFERHTSSTEMAVGNENILQSTVHTVSLNNRGNACQEAGSGSIHEVCSTGDSFPGQLGRNRGPKVNTVPPLDSMQPGVCQQSVPVSDKYLEIKKQEGEAVCADFSPCLFSDHLEQSMSGKVFQVCSETPDDLLDDVEIQGHTSFGEGDIMERSAVFNGSILRRESSRSPSPVTHASKSQSLHRASRKLESSEESDSTEDEDLPCFQHLLSRISNTPELTRCSSAVTQRMPEKAEGTQAPWKGSSSDCNNEVIMIEASQEHQFSEDPRCSGSMFSSQHSAAQGSTANANSQDSNFIPPSKQRSHQCGNEEAFLSDKELISDNEEMATCLEEDNDQEEDSIIPDSEASGYESETNLSEDCSQSDILTTQQRATMKYNLIKLQQEMAHLEAVLEQRGNQPSGHSPSLLADPCALEDLPDLEPNMSGAAILTSKNINENPVSQNLKSACDDKFQLQHLEGPTSGDDESGMGRPSPFKSPLAGSRGSAHGCSRHLQKRNSPSQEELLQPAGSEASSEPHNSTGQSCLPRRELEGTPYLGSGISLFSSRDPESESPKEPAHIGTTPASTSALKIPQGQVAFRSAAAAGADKAVVGIVSKIKPELTSSEERADRDISMVVSGLTPKEVMTVQKFAEKYRLTLTDAITEETTHVIIKTDAEFVCERTLKYFLGIAGGKWIVSYSWVVRSIQERRLLNVHEFEVKGDVVTGRNHQGPRRSRESREKLFKGLQVYCCEPFTNMPKDELERMLQLCGASVVKELPSLTHDTGAHLVVIVQPSAWTEDSNCPDIGQLCKARLVMWDWVLDSLSSYRCRDLDAYLVQNITCDSSEPQDSND

Target ORF information:

RefSeq Version	XM_030245495.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus breast cancer 1, early onset (Brca1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245495.1

ORF Insert Sequence:

ATGGATTTAT CTGCCGTCCA AATTCAAGAA GTACAAAATG TCCTTCATGC TATGCAGAAA 
ATCTTAGAGT GTCCGATCTG TTTGGAACTG ATCAAAGAAC CTGTTTCCAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTTAACC AGAAGAAAGG GCCTTCACAA 
TGTCCTTTGT GTAAGAATGA GATAACCAAA AGGAGCCTAC AGGGAAGCAC AAGGTTTAGT 
CAGCTTGCTG AAGAGCTGCT GAGAATAATG GCTGCTTTTG AGCTTGACAC GGGAATGCAG 
CTTACAAATG GTTTTAGTTT TTCAAAAAAG AGAAATAATT CTTGTGAGCG TTTGAATGAG 
GAGGCGTCGA TCATCCAGAG CGTGGGCTAC CGGAACCGTG TCAGAAGGCT TCCCCAGGTC 
GAACCTGGAA ATGCCACCTT GAAGGACAGC CTAGGTGTCC AGCTGTCTAA CCTTGGAATC 
GTGAGATCAG TGAAGAAAAA CAGGCAGACC CAACCTCGAA AGAAATCTGT CTACATTGAA 
CTAGACTCTG ATTCTTCTGA AGAGACAGTA ACTAAGCCAG GTGATTGCAG TGTGAGAGAC 
CAGGAATTGT TACAGACCGC CCCTCAAGAA GCTGGAGATG AAGGCAAGCT GCACTCTGCA 
GAAGAGGCTG CTTGTGAGTT TTCTGAGGGC ATAAGAAACA TTGAACATCA TCAATGCAGT 
GATGATTTAA ACCCTACTGA GAATCATGCA ACTGAAAGGC ATCCAGAAAA ATGTCAGAGT 
ATTTCTATTT CAAATGTGTG TGTGGAGCCA TGTGGCACAG ATGCTCATGC CAGCTCATTA 
CAGCCTGAGA CCAGCAGTTT ATTGCTCATT GAAGACAGAA TGAATGCAGA AAAGGCTGAA 
TTCTGTAATA AAAGCAAACA GCCTGGCATA GCAGTGAGCC AGCAGAGCAG ATGGGCTGCA 
AGTAAAGGAA CATGTAACGA CAGGCAGGTT CCCAGCACTG GGGAAAAGGT AGGTCCAAAC 
GCTGACTCCC TTAGTGATAG AGAGAAGTGG ACTCACCCGC AAAGTCTGTG CCCTGAGAAT 
TCTGGAGCTA CCACCGATGT TCCTTGGATA ACACTAAATA GCAGCGTTCA GAAAGTTAAT 
GAGTGGTTTT CCAGAACTGG TGAAATGTTA ACTTCTGACA GCGCATCTGC CAGGAGGCAC 
GAGTCAAATG CTGAAGCAGC TGTTGTGTTG GAAGTTTCAA ACGAAGTGGA TGGGGGTTTT 
AGTTCTTCAA GGAAAACAGA CTTAGTAACC CCCGACCCCC ATCATACTTT AATGTGTAAA 
AGTGGAAGAG ACTTCTCCAA ACCAGTAGAG GATAATATCA GTGATAAAAT ATTTGGGAAA 
TCCTATCAGA GAAAGGGAAG CCGCCCTCAC CTGAACCATG TGACTGAAAT TATAGGCACA 
TTTATTACAG AACCACAGAT AACACAAGAG CAGCCCTTCA CAAATAAATT AAAACGTAAG 
AGAAGTACAT CCCTTCAACC TGAGGACTTC ATCAAGAAAG CAGATTCAGC AGGTGTTCAA 
AGGACTCCTG ACAACATAAA TCAGGGAACT GACCTAATGG AGCCAAATGA GCAAGCAGTG 
AGTACTACCA GTAACTGTCA GGAGAACAAA ATAGCAGGTA GTAATCTCCA GAAAGAGAAA 
AGCGCTCATC CAACTGAATC ATTGAGAAAG GAACCTGCTT CCACAGCAGG AGCCAAATCT 
ATAAGCAACA GTGTAAGTGA TTTGGAGGTA GAATTAAACG TCCACAGTTC AAAAGCACCT 
AAGAAAAATA GGCTGAGGAG GAAGTCTTCT ATCAGGTGTG CTCTTCCACT TGAACCAATC 
AGTAGAAATC CAAGCCCACC TACTTGTGCT GAGCTTCAAA TCGATAGTTG TGGTAGCAGT 
GAAGAAACAA AGAAAAACCA TTCCAACCAA CAGCCAGCCG GGCACCTTAG AGAGCCTCAA 
CTCATCGAAG ACACTGAACC TGCAGCGGAT GCCAAGAAGA ACGAGCCAAA TGAACACATA 
AGGAAGAGAC GTGCCAGCGA TGCTTTCCCA GAAGAGAAAT TAATGAACAA AGCTGGTTTA 
TTAACTAGCT GTTCAAGTCC TAGAAAATCT CAAGGGCCTG TCAATCCCAG CCCTCAGAGA 
ACAGGAACAG AGCAACTTGA AACACGCCAA ATGTCTGACA GTGCCAAAGA ACTCGGGGAT 
CGGGTCCTAG GAGGAGAGCC CAGTGGCAAA ACCACTGACC GATCTGAGGA GAGCACCAGC 
GTATCCTTGG TATCTGACAC TGACTACGAC ACTCAGAACA GTGTCTCAGT CCTGGACGCT 
CACACTGTCA GATATGCAAG AACAGGATCC GCTCAGTGTA TGACTCAGTT TGTAGCAAGC 
GAAAACCCCA AGGAACTCGT CCATGGCTCT AACAATGCTG GGAGTGGCAC AGAGGGTCTC 
AAGCCCCCCT TGAGACACGC GCTTAACCTC AGTCAGGAGA AAGTAGAAAT GGAAGACAGT 
GAACTTGATA CTCAGTATTT GCAGAATACA TTTCAAGTTT CAAAGCGTCA GTCATTTGCT 
TTATTTTCAA AACCTAGAAG TCCCCAAAAG GACTGTGCTC ACTCTGTGCC CTCAAAGGAA 
CTGAGTCCAA AGGTGACAGC TAAAGGTAAA CAAAAAGAAC GTCAGGGACA GGAAGAATTT 
GAAATCAGTC ACGTACAAGC AGTTGCGGCC ACAGTGGGCT TACCTGTGCC CTGTCAAGAA 
GGTAAGCTAG CTGCTGATAC AATGTGTGAT AGAGGTTGTA GGCTTTGTCC ATCATCTCAT 
TACAGAAGCG GGGAGAATGG ACTCAGCGCC ACAGGTAAAT CAGGAATTTC ACAAAACTCA 
CATTTTAAAC AATCAGTTTC TCCCATCAGG TCATCTATAA AAACTGACAA TAGGAAACCT 
CTGACAGAGG GACGATTTGA GAGACATACA TCATCAACTG AGATGGCGGT GGGAAATGAG 
AACATTCTTC AGAGTACAGT GCACACAGTT AGCCTGAATA ACAGAGGAAA TGCTTGTCAA 
GAAGCCGGCT CGGGCAGTAT TCATGAAGTA TGTTCCACTG GTGACTCCTT CCCAGGACAA 
CTAGGTAGAA ACAGAGGGCC TAAGGTGAAC ACTGTGCCTC CATTAGATAG TATGCAGCCT 
GGTGTCTGTC AGCAAAGTGT TCCTGTAAGT GATAAGTATC TTGAAATAAA AAAGCAGGAG 
GGTGAGGCTG TCTGTGCAGA CTTCTCTCCA TGTCTATTCT CAGACCATCT TGAGCAATCT 
ATGAGTGGTA AGGTTTTTCA GGTTTGCTCT GAGACACCTG ATGACCTGCT GGATGATGTT 
GAAATACAGG GACATACTAG CTTTGGTGAA GGTGACATAA TGGAGAGATC TGCTGTCTTT 
AACGGAAGCA TCCTGAGAAG GGAGTCCAGT AGGAGCCCTA GTCCTGTAAC CCATGCATCG 
AAGTCTCAGA GTCTCCACAG AGCGTCTAGG AAATTAGAAT CGTCAGAAGA GAGCGACTCC 
ACTGAGGATG AAGATCTTCC CTGCTTCCAA CACTTACTGA GCAGAATAAG CAACACACCT 
GAGCTTACCA GATGCAGCAG TGCTGTGACA CAGCGTATGC CAGAGAAAGC GGAGGGGACC 
CAAGCACCAT GGAAGGGTAG CAGCAGTGAC TGCAATAATG AGGTGATCAT GATAGAGGCA 
TCTCAGGAGC ATCAGTTTAG TGAGGATCCA AGATGCTCTG GCAGCATGTT CTCTTCACAG 
CACAGTGCTG CCCAAGGGTC AACTGCAAAT GCAAACTCCC AGGATTCCAA TTTTATTCCA 
CCTTCCAAAC AGAGGAGTCA CCAGTGTGGG AATGAGGAAG CTTTCCTAAG TGACAAGGAA 
TTGATTTCAG ATAACGAGGA AATGGCAACT TGCCTAGAAG AGGATAATGA CCAAGAAGAG 
GATAGTATAA TCCCAGATTC AGAGGCATCC GGATACGAGA GTGAAACAAA CCTTTCTGAA 
GACTGCTCGC AGAGTGATAT TTTAACCACT CAGCAGCGGG CGACCATGAA GTATAACCTG 
ATAAAGCTGC AGCAGGAAAT GGCTCACCTG GAAGCTGTGC TGGAGCAGCG TGGGAACCAG 
CCTTCTGGCC ACTCCCCTTC CCTCCTAGCG GACCCTTGTG CCCTGGAAGA CCTGCCAGAT 
CTGGAACCAA ACATGTCAGG AGCAGCAATT TTAACTTCAA AGAACATTAA TGAGAATCCT 
GTAAGCCAAA ATTTGAAGAG CGCTTGTGAT GACAAATTCC AACTACAACA TCTGGAGGGT 
CCCACCAGTG GAGATGACGA GTCAGGGATG GGAAGGCCTT CCCCTTTTAA ATCTCCGTTG 
GCAGGCAGTA GGGGCTCTGC ACATGGCTGC TCTAGGCATC TTCAAAAGAG AAACTCCCCC 
TCTCAGGAGG AGCTCCTCCA GCCTGCTGGA TCAGAGGCGT CATCTGAGCC ACACAATTCA 
ACAGGGCAGT CTTGCCTGCC AAGGCGAGAG CTAGAAGGAA CCCCATACCT GGGATCTGGA 
ATCAGCCTTT TCTCTAGTAG AGACCCCGAA TCTGAGTCCC CTAAAGAGCC AGCCCACATT 
GGCACCACAC CAGCTTCAAC CTCTGCACTG AAAATACCCC AAGGTCAAGT TGCTTTCCGG 
AGTGCAGCTG CTGCTGGTGC TGATAAAGCA GTGGTAGGAA TTGTGAGCAA GATAAAGCCG 
GAATTGACAT CTTCAGAAGA AAGAGCGGAT AGAGACATAT CCATGGTGGT GTCAGGCTTG 
ACCCCCAAAG AAGTAATGAC CGTGCAAAAG TTTGCTGAAA AATACCGCCT CACTTTAACT 
GACGCAATTA CTGAGGAGAC TACACATGTA ATTATAAAAA CAGATGCGGA GTTTGTGTGT 
GAGCGGACAC TGAAATATTT TCTGGGCATT GCAGGAGGAA AGTGGATAGT TAGCTATTCA 
TGGGTGGTCC GGTCTATCCA AGAAAGAAGA CTTCTGAATG TGCATGAATT TGAAGTCAAA 
GGAGATGTTG TGACTGGAAG AAATCACCAA GGTCCAAGGC GATCCAGAGA ATCCCGGGAA 
AAGCTCTTCA AGGGCCTACA GGTCTATTGT TGTGAGCCCT TCACCAACAT GCCCAAAGAT 
GAGCTGGAGA GGATGCTGCA GCTGTGTGGG GCTTCCGTGG TGAAGGAGCT TCCATCGCTC 
ACCCATGACA CAGGTGCTCA TCTAGTTGTG ATCGTGCAGC CAAGCGCCTG GACAGAAGAC 
AGCAACTGCC CAGATATTGG GCAGCTGTGC AAGGCACGTC TTGTGATGTG GGACTGGGTG 
TTGGACAGTC TATCCAGCTA CCGGTGTCGG GATCTGGATG CCTACCTGGT ACAGAATATC 
ACCTGTGACA GTAGTGAGCC ACAAGACTCC AATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brca1 ( XM_006532064.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532127.1 Mus musculus breast cancer 1, early onset (Brca1), transcript variant X2, mRNA.

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CloneID	OMu45459
Clone ID Related Accession (Same CDS sequence)	XM_006532064.3 , XM_006532064.4
Accession Version	XM_006532064.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	breast cancer type 1 susceptibility protein homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532064.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAT CTGCCGTCCA AATTCAAGAA GTACAAAATG TCCTTCATGC TATGCAGAAA 
ATCTTAGAGT GTCCGATCTG TTTGGAACTG ATCAAAGAAC CTGTTTCCAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTTAACC AGAAGAAAGG GCCTTCACAA 
TGTCCTTTGT GTAAGAATGA GATAACCAAA AGGAGCCTAC AGGGAAGCAC AAGGTTTAGT 
CAGCTTGCTG AAGAGCTGCT GAGAATAATG GCTGCTTTTG AGCTTGACAC GGGAATGCAG 
CTTACAAATG GTTTTAGTTT TTCAAAAAAG AGAAATAATT CTTGTGAGCG TTTGAATGAG 
GAGGCGTCGA TCATCCAGAG CGTGGGCTAC CGGAACCGTG TCAGAAGGCT TCCCCAGGTC 
GAACCTGGAA ATGCCACCTT GAAGGACAGC CTAGGTGTCC AGCTGTCTAA CCTTGGAATC 
GTGAGATCAG TGAAGAAAAA CAGGCAGACC CAACCTCGAA AGAAATCTGT CTACATTGAA 
CTAGACTCTG ATTCTTCTGA AGAGACAGTA ACTAAGCCAG GTGATTGCAG TGTGAGAGAC 
CAGGAATTGT TACAGACCGC CCCTCAAGAA GCTGGAGATG AAGGCAAGCT GCACTCTGCA 
GAAGAGGCTG CTTGTGAGTT TTCTGAGGGC ATAAGAAACA TTGAACATCA TCAATGCAGT 
GATGATTTAA ACCCTACTGA GAATCATGCA ACTGAAAGGC ATCCAGAAAA ATGTCAGAGT 
ATTTCTATTT CAAATGTGTG TGTGGAGCCA TGTGGCACAG ATGCTCATGC CAGCTCATTA 
CAGCCTGAGA CCAGCAGTTT ATTGCTCATT GAAGACAGAA TGAATGCAGA AAAGGCTGAA 
TTCTGTAATA AAAGCAAACA GCCTGGCATA GCAGTGAGCC AGCAGAGCAG ATGGGCTGCA 
AGTAAAGGAA CATGTAACGA CAGGCAGGTT CCCAGCACTG GGGAAAAGGT AGGTCCAAAC 
GCTGACTCCC TTAGTGATAG AGAGAAGTGG ACTCACCCGC AAAGTCTGTG CCCTGAGAAT 
TCTGGAGCTA CCACCGATGT TCCTTGGATA ACACTAAATA GCAGCGTTCA GAAAGTTAAT 
GAGTGGTTTT CCAGAACTGG TGAAATGTTA ACTTCTGACA GCGCATCTGC CAGGAGGCAC 
GAGTCAAATG CTGAAGCAGC TGTTGTGTTG GAAGTTTCAA ACGAAGTGGA TGGGGGTTTT 
AGTTCTTCAA GGAAAACAGA CTTAGTAACC CCCGACCCCC ATCATACTTT AATGTGTAAA 
AGTGGAAGAG ACTTCTCCAA ACCAGTAGAG GATAATATCA GTGATAAAAT ATTTGGGAAA 
TCCTATCAGA GAAAGGGAAG CCGCCCTCAC CTGAACCATG TGACTGAAAT TATAGGCACA 
TTTATTACAG AACCACAGAT AACACAAGAG CAGCCCTTCA CAAATAAATT AAAACGTAAG 
AGAAGTACAT CCCTTCAACC TGAGGACTTC ATCAAGAAAG CAGATTCAGC AGGTGTTCAA 
AGGACTCCTG ACAACATAAA TCAGGGAACT GACCTAATGG AGCCAAATGA GCAAGCAGTG 
AGTACTACCA GTAACTGTCA GGAGAACAAA ATAGCAGGTA GTAATCTCCA GAAAGAGAAA 
AGCGCTCATC CAACTGAATC ATTGAGAAAG GAACCTGCTT CCACAGCAGG AGCCAAATCT 
ATAAGCAACA GTGTAAGTGA TTTGGAGGTA GAATTAAACG TCCACAGTTC AAAAGCACCT 
AAGAAAAATA GGCTGAGGAG GAAGTCTTCT ATCAGGTGTG CTCTTCCACT TGAACCAATC 
AGTAGAAATC CAAGCCCACC TACTTGTGCT GAGCTTCAAA TCGATAGTTG TGGTAGCAGT 
GAAGAAACAA AGAAAAACCA TTCCAACCAA CAGCCAGCCG GGCACCTTAG AGAGCCTCAA 
CTCATCGAAG ACACTGAACC TGCAGCGGAT GCCAAGAAGA ACGAGCCAAA TGAACACATA 
AGGAAGAGAC GTGCCAGCGA TGCTTTCCCA GAAGAGAAAT TAATGAACAA AGCTGGTTTA 
TTAACTAGCT GTTCAAGTCC TAGAAAATCT CAAGGGCCTG TCAATCCCAG CCCTCAGAGA 
ACAGGAACAG AGCAACTTGA AACACGCCAA ATGTCTGACA GTGCCAAAGA ACTCGGGGAT 
CGGGTCCTAG GAGGAGAGCC CAGTGGCAAA ACCACTGACC GATCTGAGGA GAGCACCAGC 
GTATCCTTGG TATCTGACAC TGACTACGAC ACTCAGAACA GTGTCTCAGT CCTGGACGCT 
CACACTGTCA GATATGCAAG AACAGGATCC GCTCAGTGTA TGACTCAGTT TGTAGCAAGC 
GAAAACCCCA AGGAACTCGT CCATGGCTCT AACAATGCTG GGAGTGGCAC AGAGGGTCTC 
AAGCCCCCCT TGAGACACGC GCTTAACCTC AGTCAGGAGA AAGTAGAAAT GGAAGACAGT 
GAACTTGATA CTCAGTATTT GCAGAATACA TTTCAAGTTT CAAAGCGTCA GTCATTTGCT 
TTATTTTCAA AACCTAGAAG TCCCCAAAAG GACTGTGCTC ACTCTGTGCC CTCAAAGGAA 
CTGAGTCCAA AGGTGACAGC TAAAGGTAAA CAAAAAGAAC GTCAGGGACA GGAAGAATTT 
GAAATCAGTC ACGTACAAGC AGTTGCGGCC ACAGTGGGCT TACCTGTGCC CTGTCAAGAA 
GTTTCTCCCA TCAGGTCATC TATAAAAACT GACAATAGGA AACCTCTGAC AGAGGGACGA 
TTTGAGAGAC ATACATCATC AACTGAGATG GCGGTGGGAA ATGAGAACAT TCTTCAGAGT 
ACAGTGCACA CAGTTAGCCT GAATAACAGA GGAAATGCTT GTCAAGAAGC CGGCTCGGGC 
AGTATTCATG AAGTATGTTC CACTGGTGAC TCCTTCCCAG GACAACTAGG TAGAAACAGA 
GGGCCTAAGG TGAACACTGT GCCTCCATTA GATAGTATGC AGCCTGGTGT CTGTCAGCAA 
AGTGTTCCTG TAAGTGATAA GTATCTTGAA ATAAAAAAGC AGGAGGGTGA GGCTGTCTGT 
GCAGACTTCT CTCCATGTCT ATTCTCAGAC CATCTTGAGC AATCTATGAG TGGTAAGGTT 
TTTCAGGTTT GCTCTGAGAC ACCTGATGAC CTGCTGGATG ATGTTGAAAT ACAGGGACAT 
ACTAGCTTTG GTGAAGGTGA CATAATGGAG AGATCTGCTG TCTTTAACGG AAGCATCCTG 
AGAAGGGAGT CCAGTAGGAG CCCTAGTCCT GTAACCCATG CATCGAAGTC TCAGAGTCTC 
CACAGAGCGT CTAGGAAATT AGAATCGTCA GAAGAGAGCG ACTCCACTGA GGATGAAGAT 
CTTCCCTGCT TCCAACACTT ACTGAGCAGA ATAAGCAACA CACCTGAGCT TACCAGATGC 
AGCAGTGCTG TGACACAGCG TATGCCAGAG AAAGCGGAGG GGACCCAAGC ACCATGGAAG 
GGTAGCAGCA GTGACTGCAA TAATGAGGTG ATCATGATAG AGGCATCTCA GGAGCATCAG 
TTTAGTGAGG ATCCAAGATG CTCTGGCAGC ATGTTCTCTT CACAGCACAG TGCTGCCCAA 
GGGTCAACTG CAAATGCAAA CTCCCAGGAT TCCAATTTTA TTCCACCTTC CAAACAGAGG 
AGTCACCAGT GTGGGAATGA GGAAGCTTTC CTAAGTGACA AGGAATTGAT TTCAGATAAC 
GAGGAAATGG CAACTTGCCT AGAAGAGGAT AATGACCAAG AAGAGGATAG TATAATCCCA 
GATTCAGAGG CATCCGGATA CGAGAGTGAA ACAAACCTTT CTGAAGACTG CTCGCAGAGT 
GATATTTTAA CCACTCAGCA GCGGGCGACC ATGAAGTATA ACCTGATAAA GCTGCAGCAG 
GAAATGGCTC ACCTGGAAGC TGTGCTGGAG CAGCGTGGGA ACCAGCCTTC TGGCCACTCC 
CCTTCCCTCC TAGCGGACCC TTGTGCCCTG GAAGACCTGC CAGATCTGGA ACCAAACATG 
TCAGGAGCAG CAATTTTAAC TTCAAAGAAC ATTAATGAGA ATCCTGTAAG CCAAAATTTG 
AAGAGCGCTT GTGATGACAA ATTCCAACTA CAACATCTGG AGGGTCCCAC CAGTGGAGAT 
GACGAGTCAG GGATGGGAAG GCCTTCCCCT TTTAAATCTC CGTTGGCAGG CAGTAGGGGC 
TCTGCACATG GCTGCTCTAG GCATCTTCAA AAGAGAAACT CCCCCTCTCA GGAGGAGCTC 
CTCCAGCCTG CTGGATCAGA GGCGTCATCT GAGCCACACA ATTCAACAGG GCAGTCTTGC 
CTGCCAAGGC GAGAGCTAGA AGGAACCCCA TACCTGGGAT CTGGAATCAG CCTTTTCTCT 
AGTAGAGACC CCGAATCTGA GTCCCCTAAA GAGCCAGCCC ACATTGGCAC CACACCAGCT 
TCAACCTCTG CACTGAAAAT ACCCCAAGGT CAAGTTGCTT TCCGGAGTGC AGCTGCTGCT 
GGTGCTGATA AAGCAGTGGT AGGAATTGTG AGCAAGATAA AGCCGGAATT GACATCTTCA 
GAAGAAAGAG CGGATAGAGA CATATCCATG GTGGTGTCAG GCTTGACCCC CAAAGAAGTA 
ATGACCGTGC AAAAGTTTGC TGAAAAATAC CGCCTCACTT TAACTGACGC AATTACTGAG 
GAGACTACAC ATGTAATTAT AAAAACAGAT GCGGAGTTTG TGTGTGAGCG GACACTGAAA 
TATTTTCTGG GCATTGCAGG AGGAAAGTGG ATAGTTAGCT ATTCATGGGT GGTCCGGTCT 
ATCCAAGAAA GAAGACTTCT GAATGTGCAT GAATTTGAAG TCAAAGGAGA TGTTGTGACT 
GGAAGAAATC ACCAAGGTCC AAGGCGATCC AGAGAATCCC GGGAAAAGCT CTTCAAGGGC 
CTACAGGTCT ATTGTTGTGA GCCCTTCACC AACATGCCCA AAGATGAGCT GGAGAGGATG 
CTGCAGCTGT GTGGGGCTTC CGTGGTGAAG GAGCTTCCAT CGCTCACCCA TGACACAGGT 
GCTCATCTAG TTGTGATCGT GCAGCCAAGC GCCTGGACAG AAGACAGCAA CTGCCCAGAT 
ATTGGGCAGC TGTGCAAGGC ACGTCTTGTG ATGTGGGACT GGGTGTTGGA CAGTCTATCC 
AGCTACCGGT GTCGGGATCT GGATGCCTAC CTGGTACAGA ATATCACCTG TGACAGTAGT 
GAGCCACAAG ACTCCAATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532127.1
CDS	204..5507
Translation	MDLSAVQIQEVQNVLHAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNEITKRSLQGSTRFSQLAEELLRIMAAFELDTGMQLTNGFSFSKKRNNSCERLNEEASIIQSVGYRNRVRRLPQVEPGNATLKDSLGVQLSNLGIVRSVKKNRQTQPRKKSVYIELDSDSSEETVTKPGDCSVRDQELLQTAPQEAGDEGKLHSAEEAACEFSEGIRNIEHHQCSDDLNPTENHATERHPEKCQSISISNVCVEPCGTDAHASSLQPETSSLLLIEDRMNAEKAEFCNKSKQPGIAVSQQSRWAASKGTCNDRQVPSTGEKVGPNADSLSDREKWTHPQSLCPENSGATTDVPWITLNSSVQKVNEWFSRTGEMLTSDSASARRHESNAEAAVVLEVSNEVDGGFSSSRKTDLVTPDPHHTLMCKSGRDFSKPVEDNISDKIFGKSYQRKGSRPHLNHVTEIIGTFITEPQITQEQPFTNKLKRKRSTSLQPEDFIKKADSAGVQRTPDNINQGTDLMEPNEQAVSTTSNCQENKIAGSNLQKEKSAHPTESLRKEPASTAGAKSISNSVSDLEVELNVHSSKAPKKNRLRRKSSIRCALPLEPISRNPSPPTCAELQIDSCGSSEETKKNHSNQQPAGHLREPQLIEDTEPAADAKKNEPNEHIRKRRASDAFPEEKLMNKAGLLTSCSSPRKSQGPVNPSPQRTGTEQLETRQMSDSAKELGDRVLGGEPSGKTTDRSEESTSVSLVSDTDYDTQNSVSVLDAHTVRYARTGSAQCMTQFVASENPKELVHGSNNAGSGTEGLKPPLRHALNLSQEKVEMEDSELDTQYLQNTFQVSKRQSFALFSKPRSPQKDCAHSVPSKELSPKVTAKGKQKERQGQEEFEISHVQAVAATVGLPVPCQEVSPIRSSIKTDNRKPLTEGRFERHTSSTEMAVGNENILQSTVHTVSLNNRGNACQEAGSGSIHEVCSTGDSFPGQLGRNRGPKVNTVPPLDSMQPGVCQQSVPVSDKYLEIKKQEGEAVCADFSPCLFSDHLEQSMSGKVFQVCSETPDDLLDDVEIQGHTSFGEGDIMERSAVFNGSILRRESSRSPSPVTHASKSQSLHRASRKLESSEESDSTEDEDLPCFQHLLSRISNTPELTRCSSAVTQRMPEKAEGTQAPWKGSSSDCNNEVIMIEASQEHQFSEDPRCSGSMFSSQHSAAQGSTANANSQDSNFIPPSKQRSHQCGNEEAFLSDKELISDNEEMATCLEEDNDQEEDSIIPDSEASGYESETNLSEDCSQSDILTTQQRATMKYNLIKLQQEMAHLEAVLEQRGNQPSGHSPSLLADPCALEDLPDLEPNMSGAAILTSKNINENPVSQNLKSACDDKFQLQHLEGPTSGDDESGMGRPSPFKSPLAGSRGSAHGCSRHLQKRNSPSQEELLQPAGSEASSEPHNSTGQSCLPRRELEGTPYLGSGISLFSSRDPESESPKEPAHIGTTPASTSALKIPQGQVAFRSAAAAGADKAVVGIVSKIKPELTSSEERADRDISMVVSGLTPKEVMTVQKFAEKYRLTLTDAITEETTHVIIKTDAEFVCERTLKYFLGIAGGKWIVSYSWVVRSIQERRLLNVHEFEVKGDVVTGRNHQGPRRSRESREKLFKGLQVYCCEPFTNMPKDELERMLQLCGASVVKELPSLTHDTGAHLVVIVQPSAWTEDSNCPDIGQLCKARLVMWDWVLDSLSSYRCRDLDAYLVQNITCDSSEPQDSND

Target ORF information:

RefSeq Version	XM_006532064.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus breast cancer 1, early onset (Brca1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532064.4

ORF Insert Sequence:

ATGGATTTAT CTGCCGTCCA AATTCAAGAA GTACAAAATG TCCTTCATGC TATGCAGAAA 
ATCTTAGAGT GTCCGATCTG TTTGGAACTG ATCAAAGAAC CTGTTTCCAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTTAACC AGAAGAAAGG GCCTTCACAA 
TGTCCTTTGT GTAAGAATGA GATAACCAAA AGGAGCCTAC AGGGAAGCAC AAGGTTTAGT 
CAGCTTGCTG AAGAGCTGCT GAGAATAATG GCTGCTTTTG AGCTTGACAC GGGAATGCAG 
CTTACAAATG GTTTTAGTTT TTCAAAAAAG AGAAATAATT CTTGTGAGCG TTTGAATGAG 
GAGGCGTCGA TCATCCAGAG CGTGGGCTAC CGGAACCGTG TCAGAAGGCT TCCCCAGGTC 
GAACCTGGAA ATGCCACCTT GAAGGACAGC CTAGGTGTCC AGCTGTCTAA CCTTGGAATC 
GTGAGATCAG TGAAGAAAAA CAGGCAGACC CAACCTCGAA AGAAATCTGT CTACATTGAA 
CTAGACTCTG ATTCTTCTGA AGAGACAGTA ACTAAGCCAG GTGATTGCAG TGTGAGAGAC 
CAGGAATTGT TACAGACCGC CCCTCAAGAA GCTGGAGATG AAGGCAAGCT GCACTCTGCA 
GAAGAGGCTG CTTGTGAGTT TTCTGAGGGC ATAAGAAACA TTGAACATCA TCAATGCAGT 
GATGATTTAA ACCCTACTGA GAATCATGCA ACTGAAAGGC ATCCAGAAAA ATGTCAGAGT 
ATTTCTATTT CAAATGTGTG TGTGGAGCCA TGTGGCACAG ATGCTCATGC CAGCTCATTA 
CAGCCTGAGA CCAGCAGTTT ATTGCTCATT GAAGACAGAA TGAATGCAGA AAAGGCTGAA 
TTCTGTAATA AAAGCAAACA GCCTGGCATA GCAGTGAGCC AGCAGAGCAG ATGGGCTGCA 
AGTAAAGGAA CATGTAACGA CAGGCAGGTT CCCAGCACTG GGGAAAAGGT AGGTCCAAAC 
GCTGACTCCC TTAGTGATAG AGAGAAGTGG ACTCACCCGC AAAGTCTGTG CCCTGAGAAT 
TCTGGAGCTA CCACCGATGT TCCTTGGATA ACACTAAATA GCAGCGTTCA GAAAGTTAAT 
GAGTGGTTTT CCAGAACTGG TGAAATGTTA ACTTCTGACA GCGCATCTGC CAGGAGGCAC 
GAGTCAAATG CTGAAGCAGC TGTTGTGTTG GAAGTTTCAA ACGAAGTGGA TGGGGGTTTT 
AGTTCTTCAA GGAAAACAGA CTTAGTAACC CCCGACCCCC ATCATACTTT AATGTGTAAA 
AGTGGAAGAG ACTTCTCCAA ACCAGTAGAG GATAATATCA GTGATAAAAT ATTTGGGAAA 
TCCTATCAGA GAAAGGGAAG CCGCCCTCAC CTGAACCATG TGACTGAAAT TATAGGCACA 
TTTATTACAG AACCACAGAT AACACAAGAG CAGCCCTTCA CAAATAAATT AAAACGTAAG 
AGAAGTACAT CCCTTCAACC TGAGGACTTC ATCAAGAAAG CAGATTCAGC AGGTGTTCAA 
AGGACTCCTG ACAACATAAA TCAGGGAACT GACCTAATGG AGCCAAATGA GCAAGCAGTG 
AGTACTACCA GTAACTGTCA GGAGAACAAA ATAGCAGGTA GTAATCTCCA GAAAGAGAAA 
AGCGCTCATC CAACTGAATC ATTGAGAAAG GAACCTGCTT CCACAGCAGG AGCCAAATCT 
ATAAGCAACA GTGTAAGTGA TTTGGAGGTA GAATTAAACG TCCACAGTTC AAAAGCACCT 
AAGAAAAATA GGCTGAGGAG GAAGTCTTCT ATCAGGTGTG CTCTTCCACT TGAACCAATC 
AGTAGAAATC CAAGCCCACC TACTTGTGCT GAGCTTCAAA TCGATAGTTG TGGTAGCAGT 
GAAGAAACAA AGAAAAACCA TTCCAACCAA CAGCCAGCCG GGCACCTTAG AGAGCCTCAA 
CTCATCGAAG ACACTGAACC TGCAGCGGAT GCCAAGAAGA ACGAGCCAAA TGAACACATA 
AGGAAGAGAC GTGCCAGCGA TGCTTTCCCA GAAGAGAAAT TAATGAACAA AGCTGGTTTA 
TTAACTAGCT GTTCAAGTCC TAGAAAATCT CAAGGGCCTG TCAATCCCAG CCCTCAGAGA 
ACAGGAACAG AGCAACTTGA AACACGCCAA ATGTCTGACA GTGCCAAAGA ACTCGGGGAT 
CGGGTCCTAG GAGGAGAGCC CAGTGGCAAA ACCACTGACC GATCTGAGGA GAGCACCAGC 
GTATCCTTGG TATCTGACAC TGACTACGAC ACTCAGAACA GTGTCTCAGT CCTGGACGCT 
CACACTGTCA GATATGCAAG AACAGGATCC GCTCAGTGTA TGACTCAGTT TGTAGCAAGC 
GAAAACCCCA AGGAACTCGT CCATGGCTCT AACAATGCTG GGAGTGGCAC AGAGGGTCTC 
AAGCCCCCCT TGAGACACGC GCTTAACCTC AGTCAGGAGA AAGTAGAAAT GGAAGACAGT 
GAACTTGATA CTCAGTATTT GCAGAATACA TTTCAAGTTT CAAAGCGTCA GTCATTTGCT 
TTATTTTCAA AACCTAGAAG TCCCCAAAAG GACTGTGCTC ACTCTGTGCC CTCAAAGGAA 
CTGAGTCCAA AGGTGACAGC TAAAGGTAAA CAAAAAGAAC GTCAGGGACA GGAAGAATTT 
GAAATCAGTC ACGTACAAGC AGTTGCGGCC ACAGTGGGCT TACCTGTGCC CTGTCAAGAA 
GTTTCTCCCA TCAGGTCATC TATAAAAACT GACAATAGGA AACCTCTGAC AGAGGGACGA 
TTTGAGAGAC ATACATCATC AACTGAGATG GCGGTGGGAA ATGAGAACAT TCTTCAGAGT 
ACAGTGCACA CAGTTAGCCT GAATAACAGA GGAAATGCTT GTCAAGAAGC CGGCTCGGGC 
AGTATTCATG AAGTATGTTC CACTGGTGAC TCCTTCCCAG GACAACTAGG TAGAAACAGA 
GGGCCTAAGG TGAACACTGT GCCTCCATTA GATAGTATGC AGCCTGGTGT CTGTCAGCAA 
AGTGTTCCTG TAAGTGATAA GTATCTTGAA ATAAAAAAGC AGGAGGGTGA GGCTGTCTGT 
GCAGACTTCT CTCCATGTCT ATTCTCAGAC CATCTTGAGC AATCTATGAG TGGTAAGGTT 
TTTCAGGTTT GCTCTGAGAC ACCTGATGAC CTGCTGGATG ATGTTGAAAT ACAGGGACAT 
ACTAGCTTTG GTGAAGGTGA CATAATGGAG AGATCTGCTG TCTTTAACGG AAGCATCCTG 
AGAAGGGAGT CCAGTAGGAG CCCTAGTCCT GTAACCCATG CATCGAAGTC TCAGAGTCTC 
CACAGAGCGT CTAGGAAATT AGAATCGTCA GAAGAGAGCG ACTCCACTGA GGATGAAGAT 
CTTCCCTGCT TCCAACACTT ACTGAGCAGA ATAAGCAACA CACCTGAGCT TACCAGATGC 
AGCAGTGCTG TGACACAGCG TATGCCAGAG AAAGCGGAGG GGACCCAAGC ACCATGGAAG 
GGTAGCAGCA GTGACTGCAA TAATGAGGTG ATCATGATAG AGGCATCTCA GGAGCATCAG 
TTTAGTGAGG ATCCAAGATG CTCTGGCAGC ATGTTCTCTT CACAGCACAG TGCTGCCCAA 
GGGTCAACTG CAAATGCAAA CTCCCAGGAT TCCAATTTTA TTCCACCTTC CAAACAGAGG 
AGTCACCAGT GTGGGAATGA GGAAGCTTTC CTAAGTGACA AGGAATTGAT TTCAGATAAC 
GAGGAAATGG CAACTTGCCT AGAAGAGGAT AATGACCAAG AAGAGGATAG TATAATCCCA 
GATTCAGAGG CATCCGGATA CGAGAGTGAA ACAAACCTTT CTGAAGACTG CTCGCAGAGT 
GATATTTTAA CCACTCAGCA GCGGGCGACC ATGAAGTATA ACCTGATAAA GCTGCAGCAG 
GAAATGGCTC ACCTGGAAGC TGTGCTGGAG CAGCGTGGGA ACCAGCCTTC TGGCCACTCC 
CCTTCCCTCC TAGCGGACCC TTGTGCCCTG GAAGACCTGC CAGATCTGGA ACCAAACATG 
TCAGGAGCAG CAATTTTAAC TTCAAAGAAC ATTAATGAGA ATCCTGTAAG CCAAAATTTG 
AAGAGCGCTT GTGATGACAA ATTCCAACTA CAACATCTGG AGGGTCCCAC CAGTGGAGAT 
GACGAGTCAG GGATGGGAAG GCCTTCCCCT TTTAAATCTC CGTTGGCAGG CAGTAGGGGC 
TCTGCACATG GCTGCTCTAG GCATCTTCAA AAGAGAAACT CCCCCTCTCA GGAGGAGCTC 
CTCCAGCCTG CTGGATCAGA GGCGTCATCT GAGCCACACA ATTCAACAGG GCAGTCTTGC 
CTGCCAAGGC GAGAGCTAGA AGGAACCCCA TACCTGGGAT CTGGAATCAG CCTTTTCTCT 
AGTAGAGACC CCGAATCTGA GTCCCCTAAA GAGCCAGCCC ACATTGGCAC CACACCAGCT 
TCAACCTCTG CACTGAAAAT ACCCCAAGGT CAAGTTGCTT TCCGGAGTGC AGCTGCTGCT 
GGTGCTGATA AAGCAGTGGT AGGAATTGTG AGCAAGATAA AGCCGGAATT GACATCTTCA 
GAAGAAAGAG CGGATAGAGA CATATCCATG GTGGTGTCAG GCTTGACCCC CAAAGAAGTA 
ATGACCGTGC AAAAGTTTGC TGAAAAATAC CGCCTCACTT TAACTGACGC AATTACTGAG 
GAGACTACAC ATGTAATTAT AAAAACAGAT GCGGAGTTTG TGTGTGAGCG GACACTGAAA 
TATTTTCTGG GCATTGCAGG AGGAAAGTGG ATAGTTAGCT ATTCATGGGT GGTCCGGTCT 
ATCCAAGAAA GAAGACTTCT GAATGTGCAT GAATTTGAAG TCAAAGGAGA TGTTGTGACT 
GGAAGAAATC ACCAAGGTCC AAGGCGATCC AGAGAATCCC GGGAAAAGCT CTTCAAGGGC 
CTACAGGTCT ATTGTTGTGA GCCCTTCACC AACATGCCCA AAGATGAGCT GGAGAGGATG 
CTGCAGCTGT GTGGGGCTTC CGTGGTGAAG GAGCTTCCAT CGCTCACCCA TGACACAGGT 
GCTCATCTAG TTGTGATCGT GCAGCCAAGC GCCTGGACAG AAGACAGCAA CTGCCCAGAT 
ATTGGGCAGC TGTGCAAGGC ACGTCTTGTG ATGTGGGACT GGGTGTTGGA CAGTCTATCC 
AGCTACCGGT GTCGGGATCT GGATGCCTAC CTGGTACAGA ATATCACCTG TGACAGTAGT 
GAGCCACAAG ACTCCAATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brca1 ( NM_009764.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033894.3 Mus musculus breast cancer 1, early onset (Brca1), mRNA.

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CloneID	OMu20079
Clone ID Related Accession (Same CDS sequence)	XM_030245495.1 , NM_009764.3
Accession Version	NM_009764.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	breast cancer type 1 susceptibility protein homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB662609.1, AB221611.1, AB221610.1, BC068303.1 and U36475.1. On Nov 24, 2007 this sequence version replaced NM_009764.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U31625.1, U32446.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGATTTAT CTGCCGTCCA AATTCAAGAA GTACAAAATG TCCTTCATGC TATGCAGAAA 
ATCTTAGAGT GTCCGATCTG TTTGGAACTG ATCAAAGAAC CTGTTTCCAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTTAACC AGAAGAAAGG GCCTTCACAA 
TGTCCTTTGT GTAAGAATGA GATAACCAAA AGGAGCCTAC AGGGAAGCAC AAGGTTTAGT 
CAGCTTGCTG AAGAGCTGCT GAGAATAATG GCTGCTTTTG AGCTTGACAC GGGAATGCAG 
CTTACAAATG GTTTTAGTTT TTCAAAAAAG AGAAATAATT CTTGTGAGCG TTTGAATGAG 
GAGGCGTCGA TCATCCAGAG CGTGGGCTAC CGGAACCGTG TCAGAAGGCT TCCCCAGGTC 
GAACCTGGAA ATGCCACCTT GAAGGACAGC CTAGGTGTCC AGCTGTCTAA CCTTGGAATC 
GTGAGATCAG TGAAGAAAAA CAGGCAGACC CAACCTCGAA AGAAATCTGT CTACATTGAA 
CTAGACTCTG ATTCTTCTGA AGAGACAGTA ACTAAGCCAG GTGATTGCAG TGTGAGAGAC 
CAGGAATTGT TACAGACCGC CCCTCAAGAA GCTGGAGATG AAGGCAAGCT GCACTCTGCA 
GAAGAGGCTG CTTGTGAGTT TTCTGAGGGC ATAAGAAACA TTGAACATCA TCAATGCAGT 
GATGATTTAA ACCCTACTGA GAATCATGCA ACTGAAAGGC ATCCAGAAAA ATGTCAGAGT 
ATTTCTATTT CAAATGTGTG TGTGGAGCCA TGTGGCACAG ATGCTCATGC CAGCTCATTA 
CAGCCTGAGA CCAGCAGTTT ATTGCTCATT GAAGACAGAA TGAATGCAGA AAAGGCTGAA 
TTCTGTAATA AAAGCAAACA GCCTGGCATA GCAGTGAGCC AGCAGAGCAG ATGGGCTGCA 
AGTAAAGGAA CATGTAACGA CAGGCAGGTT CCCAGCACTG GGGAAAAGGT AGGTCCAAAC 
GCTGACTCCC TTAGTGATAG AGAGAAGTGG ACTCACCCGC AAAGTCTGTG CCCTGAGAAT 
TCTGGAGCTA CCACCGATGT TCCTTGGATA ACACTAAATA GCAGCGTTCA GAAAGTTAAT 
GAGTGGTTTT CCAGAACTGG TGAAATGTTA ACTTCTGACA GCGCATCTGC CAGGAGGCAC 
GAGTCAAATG CTGAAGCAGC TGTTGTGTTG GAAGTTTCAA ACGAAGTGGA TGGGGGTTTT 
AGTTCTTCAA GGAAAACAGA CTTAGTAACC CCCGACCCCC ATCATACTTT AATGTGTAAA 
AGTGGAAGAG ACTTCTCCAA ACCAGTAGAG GATAATATCA GTGATAAAAT ATTTGGGAAA 
TCCTATCAGA GAAAGGGAAG CCGCCCTCAC CTGAACCATG TGACTGAAAT TATAGGCACA 
TTTATTACAG AACCACAGAT AACACAAGAG CAGCCCTTCA CAAATAAATT AAAACGTAAG 
AGAAGTACAT CCCTTCAACC TGAGGACTTC ATCAAGAAAG CAGATTCAGC AGGTGTTCAA 
AGGACTCCTG ACAACATAAA TCAGGGAACT GACCTAATGG AGCCAAATGA GCAAGCAGTG 
AGTACTACCA GTAACTGTCA GGAGAACAAA ATAGCAGGTA GTAATCTCCA GAAAGAGAAA 
AGCGCTCATC CAACTGAATC ATTGAGAAAG GAACCTGCTT CCACAGCAGG AGCCAAATCT 
ATAAGCAACA GTGTAAGTGA TTTGGAGGTA GAATTAAACG TCCACAGTTC AAAAGCACCT 
AAGAAAAATA GGCTGAGGAG GAAGTCTTCT ATCAGGTGTG CTCTTCCACT TGAACCAATC 
AGTAGAAATC CAAGCCCACC TACTTGTGCT GAGCTTCAAA TCGATAGTTG TGGTAGCAGT 
GAAGAAACAA AGAAAAACCA TTCCAACCAA CAGCCAGCCG GGCACCTTAG AGAGCCTCAA 
CTCATCGAAG ACACTGAACC TGCAGCGGAT GCCAAGAAGA ACGAGCCAAA TGAACACATA 
AGGAAGAGAC GTGCCAGCGA TGCTTTCCCA GAAGAGAAAT TAATGAACAA AGCTGGTTTA 
TTAACTAGCT GTTCAAGTCC TAGAAAATCT CAAGGGCCTG TCAATCCCAG CCCTCAGAGA 
ACAGGAACAG AGCAACTTGA AACACGCCAA ATGTCTGACA GTGCCAAAGA ACTCGGGGAT 
CGGGTCCTAG GAGGAGAGCC CAGTGGCAAA ACCACTGACC GATCTGAGGA GAGCACCAGC 
GTATCCTTGG TATCTGACAC TGACTACGAC ACTCAGAACA GTGTCTCAGT CCTGGACGCT 
CACACTGTCA GATATGCAAG AACAGGATCC GCTCAGTGTA TGACTCAGTT TGTAGCAAGC 
GAAAACCCCA AGGAACTCGT CCATGGCTCT AACAATGCTG GGAGTGGCAC AGAGGGTCTC 
AAGCCCCCCT TGAGACACGC GCTTAACCTC AGTCAGGAGA AAGTAGAAAT GGAAGACAGT 
GAACTTGATA CTCAGTATTT GCAGAATACA TTTCAAGTTT CAAAGCGTCA GTCATTTGCT 
TTATTTTCAA AACCTAGAAG TCCCCAAAAG GACTGTGCTC ACTCTGTGCC CTCAAAGGAA 
CTGAGTCCAA AGGTGACAGC TAAAGGTAAA CAAAAAGAAC GTCAGGGACA GGAAGAATTT 
GAAATCAGTC ACGTACAAGC AGTTGCGGCC ACAGTGGGCT TACCTGTGCC CTGTCAAGAA 
GGTAAGCTAG CTGCTGATAC AATGTGTGAT AGAGGTTGTA GGCTTTGTCC ATCATCTCAT 
TACAGAAGCG GGGAGAATGG ACTCAGCGCC ACAGGTAAAT CAGGAATTTC ACAAAACTCA 
CATTTTAAAC AATCAGTTTC TCCCATCAGG TCATCTATAA AAACTGACAA TAGGAAACCT 
CTGACAGAGG GACGATTTGA GAGACATACA TCATCAACTG AGATGGCGGT GGGAAATGAG 
AACATTCTTC AGAGTACAGT GCACACAGTT AGCCTGAATA ACAGAGGAAA TGCTTGTCAA 
GAAGCCGGCT CGGGCAGTAT TCATGAAGTA TGTTCCACTG GTGACTCCTT CCCAGGACAA 
CTAGGTAGAA ACAGAGGGCC TAAGGTGAAC ACTGTGCCTC CATTAGATAG TATGCAGCCT 
GGTGTCTGTC AGCAAAGTGT TCCTGTAAGT GATAAGTATC TTGAAATAAA AAAGCAGGAG 
GGTGAGGCTG TCTGTGCAGA CTTCTCTCCA TGTCTATTCT CAGACCATCT TGAGCAATCT 
ATGAGTGGTA AGGTTTTTCA GGTTTGCTCT GAGACACCTG ATGACCTGCT GGATGATGTT 
GAAATACAGG GACATACTAG CTTTGGTGAA GGTGACATAA TGGAGAGATC TGCTGTCTTT 
AACGGAAGCA TCCTGAGAAG GGAGTCCAGT AGGAGCCCTA GTCCTGTAAC CCATGCATCG 
AAGTCTCAGA GTCTCCACAG AGCGTCTAGG AAATTAGAAT CGTCAGAAGA GAGCGACTCC 
ACTGAGGATG AAGATCTTCC CTGCTTCCAA CACTTACTGA GCAGAATAAG CAACACACCT 
GAGCTTACCA GATGCAGCAG TGCTGTGACA CAGCGTATGC CAGAGAAAGC GGAGGGGACC 
CAAGCACCAT GGAAGGGTAG CAGCAGTGAC TGCAATAATG AGGTGATCAT GATAGAGGCA 
TCTCAGGAGC ATCAGTTTAG TGAGGATCCA AGATGCTCTG GCAGCATGTT CTCTTCACAG 
CACAGTGCTG CCCAAGGGTC AACTGCAAAT GCAAACTCCC AGGATTCCAA TTTTATTCCA 
CCTTCCAAAC AGAGGAGTCA CCAGTGTGGG AATGAGGAAG CTTTCCTAAG TGACAAGGAA 
TTGATTTCAG ATAACGAGGA AATGGCAACT TGCCTAGAAG AGGATAATGA CCAAGAAGAG 
GATAGTATAA TCCCAGATTC AGAGGCATCC GGATACGAGA GTGAAACAAA CCTTTCTGAA 
GACTGCTCGC AGAGTGATAT TTTAACCACT CAGCAGCGGG CGACCATGAA GTATAACCTG 
ATAAAGCTGC AGCAGGAAAT GGCTCACCTG GAAGCTGTGC TGGAGCAGCG TGGGAACCAG 
CCTTCTGGCC ACTCCCCTTC CCTCCTAGCG GACCCTTGTG CCCTGGAAGA CCTGCCAGAT 
CTGGAACCAA ACATGTCAGG AGCAGCAATT TTAACTTCAA AGAACATTAA TGAGAATCCT 
GTAAGCCAAA ATTTGAAGAG CGCTTGTGAT GACAAATTCC AACTACAACA TCTGGAGGGT 
CCCACCAGTG GAGATGACGA GTCAGGGATG GGAAGGCCTT CCCCTTTTAA ATCTCCGTTG 
GCAGGCAGTA GGGGCTCTGC ACATGGCTGC TCTAGGCATC TTCAAAAGAG AAACTCCCCC 
TCTCAGGAGG AGCTCCTCCA GCCTGCTGGA TCAGAGGCGT CATCTGAGCC ACACAATTCA 
ACAGGGCAGT CTTGCCTGCC AAGGCGAGAG CTAGAAGGAA CCCCATACCT GGGATCTGGA 
ATCAGCCTTT TCTCTAGTAG AGACCCCGAA TCTGAGTCCC CTAAAGAGCC AGCCCACATT 
GGCACCACAC CAGCTTCAAC CTCTGCACTG AAAATACCCC AAGGTCAAGT TGCTTTCCGG 
AGTGCAGCTG CTGCTGGTGC TGATAAAGCA GTGGTAGGAA TTGTGAGCAA GATAAAGCCG 
GAATTGACAT CTTCAGAAGA AAGAGCGGAT AGAGACATAT CCATGGTGGT GTCAGGCTTG 
ACCCCCAAAG AAGTAATGAC CGTGCAAAAG TTTGCTGAAA AATACCGCCT CACTTTAACT 
GACGCAATTA CTGAGGAGAC TACACATGTA ATTATAAAAA CAGATGCGGA GTTTGTGTGT 
GAGCGGACAC TGAAATATTT TCTGGGCATT GCAGGAGGAA AGTGGATAGT TAGCTATTCA 
TGGGTGGTCC GGTCTATCCA AGAAAGAAGA CTTCTGAATG TGCATGAATT TGAAGTCAAA 
GGAGATGTTG TGACTGGAAG AAATCACCAA GGTCCAAGGC GATCCAGAGA ATCCCGGGAA 
AAGCTCTTCA AGGGCCTACA GGTCTATTGT TGTGAGCCCT TCACCAACAT GCCCAAAGAT 
GAGCTGGAGA GGATGCTGCA GCTGTGTGGG GCTTCCGTGG TGAAGGAGCT TCCATCGCTC 
ACCCATGACA CAGGTGCTCA TCTAGTTGTG ATCGTGCAGC CAAGCGCCTG GACAGAAGAC 
AGCAACTGCC CAGATATTGG GCAGCTGTGC AAGGCACGTC TTGTGATGTG GGACTGGGTG 
TTGGACAGTC TATCCAGCTA CCGGTGTCGG GATCTGGATG CCTACCTGGT ACAGAATATC 
ACCTGTGACA GTAGTGAGCC ACAAGACTCC AATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033894.3
CDS	207..5645
Translation	MDLSAVQIQEVQNVLHAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNEITKRSLQGSTRFSQLAEELLRIMAAFELDTGMQLTNGFSFSKKRNNSCERLNEEASIIQSVGYRNRVRRLPQVEPGNATLKDSLGVQLSNLGIVRSVKKNRQTQPRKKSVYIELDSDSSEETVTKPGDCSVRDQELLQTAPQEAGDEGKLHSAEEAACEFSEGIRNIEHHQCSDDLNPTENHATERHPEKCQSISISNVCVEPCGTDAHASSLQPETSSLLLIEDRMNAEKAEFCNKSKQPGIAVSQQSRWAASKGTCNDRQVPSTGEKVGPNADSLSDREKWTHPQSLCPENSGATTDVPWITLNSSVQKVNEWFSRTGEMLTSDSASARRHESNAEAAVVLEVSNEVDGGFSSSRKTDLVTPDPHHTLMCKSGRDFSKPVEDNISDKIFGKSYQRKGSRPHLNHVTEIIGTFITEPQITQEQPFTNKLKRKRSTSLQPEDFIKKADSAGVQRTPDNINQGTDLMEPNEQAVSTTSNCQENKIAGSNLQKEKSAHPTESLRKEPASTAGAKSISNSVSDLEVELNVHSSKAPKKNRLRRKSSIRCALPLEPISRNPSPPTCAELQIDSCGSSEETKKNHSNQQPAGHLREPQLIEDTEPAADAKKNEPNEHIRKRRASDAFPEEKLMNKAGLLTSCSSPRKSQGPVNPSPQRTGTEQLETRQMSDSAKELGDRVLGGEPSGKTTDRSEESTSVSLVSDTDYDTQNSVSVLDAHTVRYARTGSAQCMTQFVASENPKELVHGSNNAGSGTEGLKPPLRHALNLSQEKVEMEDSELDTQYLQNTFQVSKRQSFALFSKPRSPQKDCAHSVPSKELSPKVTAKGKQKERQGQEEFEISHVQAVAATVGLPVPCQEGKLAADTMCDRGCRLCPSSHYRSGENGLSATGKSGISQNSHFKQSVSPIRSSIKTDNRKPLTEGRFERHTSSTEMAVGNENILQSTVHTVSLNNRGNACQEAGSGSIHEVCSTGDSFPGQLGRNRGPKVNTVPPLDSMQPGVCQQSVPVSDKYLEIKKQEGEAVCADFSPCLFSDHLEQSMSGKVFQVCSETPDDLLDDVEIQGHTSFGEGDIMERSAVFNGSILRRESSRSPSPVTHASKSQSLHRASRKLESSEESDSTEDEDLPCFQHLLSRISNTPELTRCSSAVTQRMPEKAEGTQAPWKGSSSDCNNEVIMIEASQEHQFSEDPRCSGSMFSSQHSAAQGSTANANSQDSNFIPPSKQRSHQCGNEEAFLSDKELISDNEEMATCLEEDNDQEEDSIIPDSEASGYESETNLSEDCSQSDILTTQQRATMKYNLIKLQQEMAHLEAVLEQRGNQPSGHSPSLLADPCALEDLPDLEPNMSGAAILTSKNINENPVSQNLKSACDDKFQLQHLEGPTSGDDESGMGRPSPFKSPLAGSRGSAHGCSRHLQKRNSPSQEELLQPAGSEASSEPHNSTGQSCLPRRELEGTPYLGSGISLFSSRDPESESPKEPAHIGTTPASTSALKIPQGQVAFRSAAAAGADKAVVGIVSKIKPELTSSEERADRDISMVVSGLTPKEVMTVQKFAEKYRLTLTDAITEETTHVIIKTDAEFVCERTLKYFLGIAGGKWIVSYSWVVRSIQERRLLNVHEFEVKGDVVTGRNHQGPRRSRESREKLFKGLQVYCCEPFTNMPKDELERMLQLCGASVVKELPSLTHDTGAHLVVIVQPSAWTEDSNCPDIGQLCKARLVMWDWVLDSLSSYRCRDLDAYLVQNITCDSSEPQDSND

Target ORF information:

RefSeq Version	NM_009764.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus breast cancer 1, early onset (Brca1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009764.3

ORF Insert Sequence:

ATGGATTTAT CTGCCGTCCA AATTCAAGAA GTACAAAATG TCCTTCATGC TATGCAGAAA 
ATCTTAGAGT GTCCGATCTG TTTGGAACTG ATCAAAGAAC CTGTTTCCAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTTAACC AGAAGAAAGG GCCTTCACAA 
TGTCCTTTGT GTAAGAATGA GATAACCAAA AGGAGCCTAC AGGGAAGCAC AAGGTTTAGT 
CAGCTTGCTG AAGAGCTGCT GAGAATAATG GCTGCTTTTG AGCTTGACAC GGGAATGCAG 
CTTACAAATG GTTTTAGTTT TTCAAAAAAG AGAAATAATT CTTGTGAGCG TTTGAATGAG 
GAGGCGTCGA TCATCCAGAG CGTGGGCTAC CGGAACCGTG TCAGAAGGCT TCCCCAGGTC 
GAACCTGGAA ATGCCACCTT GAAGGACAGC CTAGGTGTCC AGCTGTCTAA CCTTGGAATC 
GTGAGATCAG TGAAGAAAAA CAGGCAGACC CAACCTCGAA AGAAATCTGT CTACATTGAA 
CTAGACTCTG ATTCTTCTGA AGAGACAGTA ACTAAGCCAG GTGATTGCAG TGTGAGAGAC 
CAGGAATTGT TACAGACCGC CCCTCAAGAA GCTGGAGATG AAGGCAAGCT GCACTCTGCA 
GAAGAGGCTG CTTGTGAGTT TTCTGAGGGC ATAAGAAACA TTGAACATCA TCAATGCAGT 
GATGATTTAA ACCCTACTGA GAATCATGCA ACTGAAAGGC ATCCAGAAAA ATGTCAGAGT 
ATTTCTATTT CAAATGTGTG TGTGGAGCCA TGTGGCACAG ATGCTCATGC CAGCTCATTA 
CAGCCTGAGA CCAGCAGTTT ATTGCTCATT GAAGACAGAA TGAATGCAGA AAAGGCTGAA 
TTCTGTAATA AAAGCAAACA GCCTGGCATA GCAGTGAGCC AGCAGAGCAG ATGGGCTGCA 
AGTAAAGGAA CATGTAACGA CAGGCAGGTT CCCAGCACTG GGGAAAAGGT AGGTCCAAAC 
GCTGACTCCC TTAGTGATAG AGAGAAGTGG ACTCACCCGC AAAGTCTGTG CCCTGAGAAT 
TCTGGAGCTA CCACCGATGT TCCTTGGATA ACACTAAATA GCAGCGTTCA GAAAGTTAAT 
GAGTGGTTTT CCAGAACTGG TGAAATGTTA ACTTCTGACA GCGCATCTGC CAGGAGGCAC 
GAGTCAAATG CTGAAGCAGC TGTTGTGTTG GAAGTTTCAA ACGAAGTGGA TGGGGGTTTT 
AGTTCTTCAA GGAAAACAGA CTTAGTAACC CCCGACCCCC ATCATACTTT AATGTGTAAA 
AGTGGAAGAG ACTTCTCCAA ACCAGTAGAG GATAATATCA GTGATAAAAT ATTTGGGAAA 
TCCTATCAGA GAAAGGGAAG CCGCCCTCAC CTGAACCATG TGACTGAAAT TATAGGCACA 
TTTATTACAG AACCACAGAT AACACAAGAG CAGCCCTTCA CAAATAAATT AAAACGTAAG 
AGAAGTACAT CCCTTCAACC TGAGGACTTC ATCAAGAAAG CAGATTCAGC AGGTGTTCAA 
AGGACTCCTG ACAACATAAA TCAGGGAACT GACCTAATGG AGCCAAATGA GCAAGCAGTG 
AGTACTACCA GTAACTGTCA GGAGAACAAA ATAGCAGGTA GTAATCTCCA GAAAGAGAAA 
AGCGCTCATC CAACTGAATC ATTGAGAAAG GAACCTGCTT CCACAGCAGG AGCCAAATCT 
ATAAGCAACA GTGTAAGTGA TTTGGAGGTA GAATTAAACG TCCACAGTTC AAAAGCACCT 
AAGAAAAATA GGCTGAGGAG GAAGTCTTCT ATCAGGTGTG CTCTTCCACT TGAACCAATC 
AGTAGAAATC CAAGCCCACC TACTTGTGCT GAGCTTCAAA TCGATAGTTG TGGTAGCAGT 
GAAGAAACAA AGAAAAACCA TTCCAACCAA CAGCCAGCCG GGCACCTTAG AGAGCCTCAA 
CTCATCGAAG ACACTGAACC TGCAGCGGAT GCCAAGAAGA ACGAGCCAAA TGAACACATA 
AGGAAGAGAC GTGCCAGCGA TGCTTTCCCA GAAGAGAAAT TAATGAACAA AGCTGGTTTA 
TTAACTAGCT GTTCAAGTCC TAGAAAATCT CAAGGGCCTG TCAATCCCAG CCCTCAGAGA 
ACAGGAACAG AGCAACTTGA AACACGCCAA ATGTCTGACA GTGCCAAAGA ACTCGGGGAT 
CGGGTCCTAG GAGGAGAGCC CAGTGGCAAA ACCACTGACC GATCTGAGGA GAGCACCAGC 
GTATCCTTGG TATCTGACAC TGACTACGAC ACTCAGAACA GTGTCTCAGT CCTGGACGCT 
CACACTGTCA GATATGCAAG AACAGGATCC GCTCAGTGTA TGACTCAGTT TGTAGCAAGC 
GAAAACCCCA AGGAACTCGT CCATGGCTCT AACAATGCTG GGAGTGGCAC AGAGGGTCTC 
AAGCCCCCCT TGAGACACGC GCTTAACCTC AGTCAGGAGA AAGTAGAAAT GGAAGACAGT 
GAACTTGATA CTCAGTATTT GCAGAATACA TTTCAAGTTT CAAAGCGTCA GTCATTTGCT 
TTATTTTCAA AACCTAGAAG TCCCCAAAAG GACTGTGCTC ACTCTGTGCC CTCAAAGGAA 
CTGAGTCCAA AGGTGACAGC TAAAGGTAAA CAAAAAGAAC GTCAGGGACA GGAAGAATTT 
GAAATCAGTC ACGTACAAGC AGTTGCGGCC ACAGTGGGCT TACCTGTGCC CTGTCAAGAA 
GGTAAGCTAG CTGCTGATAC AATGTGTGAT AGAGGTTGTA GGCTTTGTCC ATCATCTCAT 
TACAGAAGCG GGGAGAATGG ACTCAGCGCC ACAGGTAAAT CAGGAATTTC ACAAAACTCA 
CATTTTAAAC AATCAGTTTC TCCCATCAGG TCATCTATAA AAACTGACAA TAGGAAACCT 
CTGACAGAGG GACGATTTGA GAGACATACA TCATCAACTG AGATGGCGGT GGGAAATGAG 
AACATTCTTC AGAGTACAGT GCACACAGTT AGCCTGAATA ACAGAGGAAA TGCTTGTCAA 
GAAGCCGGCT CGGGCAGTAT TCATGAAGTA TGTTCCACTG GTGACTCCTT CCCAGGACAA 
CTAGGTAGAA ACAGAGGGCC TAAGGTGAAC ACTGTGCCTC CATTAGATAG TATGCAGCCT 
GGTGTCTGTC AGCAAAGTGT TCCTGTAAGT GATAAGTATC TTGAAATAAA AAAGCAGGAG 
GGTGAGGCTG TCTGTGCAGA CTTCTCTCCA TGTCTATTCT CAGACCATCT TGAGCAATCT 
ATGAGTGGTA AGGTTTTTCA GGTTTGCTCT GAGACACCTG ATGACCTGCT GGATGATGTT 
GAAATACAGG GACATACTAG CTTTGGTGAA GGTGACATAA TGGAGAGATC TGCTGTCTTT 
AACGGAAGCA TCCTGAGAAG GGAGTCCAGT AGGAGCCCTA GTCCTGTAAC CCATGCATCG 
AAGTCTCAGA GTCTCCACAG AGCGTCTAGG AAATTAGAAT CGTCAGAAGA GAGCGACTCC 
ACTGAGGATG AAGATCTTCC CTGCTTCCAA CACTTACTGA GCAGAATAAG CAACACACCT 
GAGCTTACCA GATGCAGCAG TGCTGTGACA CAGCGTATGC CAGAGAAAGC GGAGGGGACC 
CAAGCACCAT GGAAGGGTAG CAGCAGTGAC TGCAATAATG AGGTGATCAT GATAGAGGCA 
TCTCAGGAGC ATCAGTTTAG TGAGGATCCA AGATGCTCTG GCAGCATGTT CTCTTCACAG 
CACAGTGCTG CCCAAGGGTC AACTGCAAAT GCAAACTCCC AGGATTCCAA TTTTATTCCA 
CCTTCCAAAC AGAGGAGTCA CCAGTGTGGG AATGAGGAAG CTTTCCTAAG TGACAAGGAA 
TTGATTTCAG ATAACGAGGA AATGGCAACT TGCCTAGAAG AGGATAATGA CCAAGAAGAG 
GATAGTATAA TCCCAGATTC AGAGGCATCC GGATACGAGA GTGAAACAAA CCTTTCTGAA 
GACTGCTCGC AGAGTGATAT TTTAACCACT CAGCAGCGGG CGACCATGAA GTATAACCTG 
ATAAAGCTGC AGCAGGAAAT GGCTCACCTG GAAGCTGTGC TGGAGCAGCG TGGGAACCAG 
CCTTCTGGCC ACTCCCCTTC CCTCCTAGCG GACCCTTGTG CCCTGGAAGA CCTGCCAGAT 
CTGGAACCAA ACATGTCAGG AGCAGCAATT TTAACTTCAA AGAACATTAA TGAGAATCCT 
GTAAGCCAAA ATTTGAAGAG CGCTTGTGAT GACAAATTCC AACTACAACA TCTGGAGGGT 
CCCACCAGTG GAGATGACGA GTCAGGGATG GGAAGGCCTT CCCCTTTTAA ATCTCCGTTG 
GCAGGCAGTA GGGGCTCTGC ACATGGCTGC TCTAGGCATC TTCAAAAGAG AAACTCCCCC 
TCTCAGGAGG AGCTCCTCCA GCCTGCTGGA TCAGAGGCGT CATCTGAGCC ACACAATTCA 
ACAGGGCAGT CTTGCCTGCC AAGGCGAGAG CTAGAAGGAA CCCCATACCT GGGATCTGGA 
ATCAGCCTTT TCTCTAGTAG AGACCCCGAA TCTGAGTCCC CTAAAGAGCC AGCCCACATT 
GGCACCACAC CAGCTTCAAC CTCTGCACTG AAAATACCCC AAGGTCAAGT TGCTTTCCGG 
AGTGCAGCTG CTGCTGGTGC TGATAAAGCA GTGGTAGGAA TTGTGAGCAA GATAAAGCCG 
GAATTGACAT CTTCAGAAGA AAGAGCGGAT AGAGACATAT CCATGGTGGT GTCAGGCTTG 
ACCCCCAAAG AAGTAATGAC CGTGCAAAAG TTTGCTGAAA AATACCGCCT CACTTTAACT 
GACGCAATTA CTGAGGAGAC TACACATGTA ATTATAAAAA CAGATGCGGA GTTTGTGTGT 
GAGCGGACAC TGAAATATTT TCTGGGCATT GCAGGAGGAA AGTGGATAGT TAGCTATTCA 
TGGGTGGTCC GGTCTATCCA AGAAAGAAGA CTTCTGAATG TGCATGAATT TGAAGTCAAA 
GGAGATGTTG TGACTGGAAG AAATCACCAA GGTCCAAGGC GATCCAGAGA ATCCCGGGAA 
AAGCTCTTCA AGGGCCTACA GGTCTATTGT TGTGAGCCCT TCACCAACAT GCCCAAAGAT 
GAGCTGGAGA GGATGCTGCA GCTGTGTGGG GCTTCCGTGG TGAAGGAGCT TCCATCGCTC 
ACCCATGACA CAGGTGCTCA TCTAGTTGTG ATCGTGCAGC CAAGCGCCTG GACAGAAGAC 
AGCAACTGCC CAGATATTGG GCAGCTGTGC AAGGCACGTC TTGTGATGTG GGACTGGGTG 
TTGGACAGTC TATCCAGCTA CCGGTGTCGG GATCTGGATG CCTACCTGGT ACAGAATATC 
ACCTGTGACA GTAGTGAGCC ACAAGACTCC AATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brca1 ( XM_006532064.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532127.1 Mus musculus breast cancer 1, early onset (Brca1), transcript variant X1, mRNA.

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CloneID	OMu45459
Clone ID Related Accession (Same CDS sequence)	XM_006532064.3 , XM_006532064.4
Accession Version	XM_006532064.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	breast cancer type 1 susceptibility protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532064.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAT CTGCCGTCCA AATTCAAGAA GTACAAAATG TCCTTCATGC TATGCAGAAA 
ATCTTAGAGT GTCCGATCTG TTTGGAACTG ATCAAAGAAC CTGTTTCCAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTTAACC AGAAGAAAGG GCCTTCACAA 
TGTCCTTTGT GTAAGAATGA GATAACCAAA AGGAGCCTAC AGGGAAGCAC AAGGTTTAGT 
CAGCTTGCTG AAGAGCTGCT GAGAATAATG GCTGCTTTTG AGCTTGACAC GGGAATGCAG 
CTTACAAATG GTTTTAGTTT TTCAAAAAAG AGAAATAATT CTTGTGAGCG TTTGAATGAG 
GAGGCGTCGA TCATCCAGAG CGTGGGCTAC CGGAACCGTG TCAGAAGGCT TCCCCAGGTC 
GAACCTGGAA ATGCCACCTT GAAGGACAGC CTAGGTGTCC AGCTGTCTAA CCTTGGAATC 
GTGAGATCAG TGAAGAAAAA CAGGCAGACC CAACCTCGAA AGAAATCTGT CTACATTGAA 
CTAGACTCTG ATTCTTCTGA AGAGACAGTA ACTAAGCCAG GTGATTGCAG TGTGAGAGAC 
CAGGAATTGT TACAGACCGC CCCTCAAGAA GCTGGAGATG AAGGCAAGCT GCACTCTGCA 
GAAGAGGCTG CTTGTGAGTT TTCTGAGGGC ATAAGAAACA TTGAACATCA TCAATGCAGT 
GATGATTTAA ACCCTACTGA GAATCATGCA ACTGAAAGGC ATCCAGAAAA ATGTCAGAGT 
ATTTCTATTT CAAATGTGTG TGTGGAGCCA TGTGGCACAG ATGCTCATGC CAGCTCATTA 
CAGCCTGAGA CCAGCAGTTT ATTGCTCATT GAAGACAGAA TGAATGCAGA AAAGGCTGAA 
TTCTGTAATA AAAGCAAACA GCCTGGCATA GCAGTGAGCC AGCAGAGCAG ATGGGCTGCA 
AGTAAAGGAA CATGTAACGA CAGGCAGGTT CCCAGCACTG GGGAAAAGGT AGGTCCAAAC 
GCTGACTCCC TTAGTGATAG AGAGAAGTGG ACTCACCCGC AAAGTCTGTG CCCTGAGAAT 
TCTGGAGCTA CCACCGATGT TCCTTGGATA ACACTAAATA GCAGCGTTCA GAAAGTTAAT 
GAGTGGTTTT CCAGAACTGG TGAAATGTTA ACTTCTGACA GCGCATCTGC CAGGAGGCAC 
GAGTCAAATG CTGAAGCAGC TGTTGTGTTG GAAGTTTCAA ACGAAGTGGA TGGGGGTTTT 
AGTTCTTCAA GGAAAACAGA CTTAGTAACC CCCGACCCCC ATCATACTTT AATGTGTAAA 
AGTGGAAGAG ACTTCTCCAA ACCAGTAGAG GATAATATCA GTGATAAAAT ATTTGGGAAA 
TCCTATCAGA GAAAGGGAAG CCGCCCTCAC CTGAACCATG TGACTGAAAT TATAGGCACA 
TTTATTACAG AACCACAGAT AACACAAGAG CAGCCCTTCA CAAATAAATT AAAACGTAAG 
AGAAGTACAT CCCTTCAACC TGAGGACTTC ATCAAGAAAG CAGATTCAGC AGGTGTTCAA 
AGGACTCCTG ACAACATAAA TCAGGGAACT GACCTAATGG AGCCAAATGA GCAAGCAGTG 
AGTACTACCA GTAACTGTCA GGAGAACAAA ATAGCAGGTA GTAATCTCCA GAAAGAGAAA 
AGCGCTCATC CAACTGAATC ATTGAGAAAG GAACCTGCTT CCACAGCAGG AGCCAAATCT 
ATAAGCAACA GTGTAAGTGA TTTGGAGGTA GAATTAAACG TCCACAGTTC AAAAGCACCT 
AAGAAAAATA GGCTGAGGAG GAAGTCTTCT ATCAGGTGTG CTCTTCCACT TGAACCAATC 
AGTAGAAATC CAAGCCCACC TACTTGTGCT GAGCTTCAAA TCGATAGTTG TGGTAGCAGT 
GAAGAAACAA AGAAAAACCA TTCCAACCAA CAGCCAGCCG GGCACCTTAG AGAGCCTCAA 
CTCATCGAAG ACACTGAACC TGCAGCGGAT GCCAAGAAGA ACGAGCCAAA TGAACACATA 
AGGAAGAGAC GTGCCAGCGA TGCTTTCCCA GAAGAGAAAT TAATGAACAA AGCTGGTTTA 
TTAACTAGCT GTTCAAGTCC TAGAAAATCT CAAGGGCCTG TCAATCCCAG CCCTCAGAGA 
ACAGGAACAG AGCAACTTGA AACACGCCAA ATGTCTGACA GTGCCAAAGA ACTCGGGGAT 
CGGGTCCTAG GAGGAGAGCC CAGTGGCAAA ACCACTGACC GATCTGAGGA GAGCACCAGC 
GTATCCTTGG TATCTGACAC TGACTACGAC ACTCAGAACA GTGTCTCAGT CCTGGACGCT 
CACACTGTCA GATATGCAAG AACAGGATCC GCTCAGTGTA TGACTCAGTT TGTAGCAAGC 
GAAAACCCCA AGGAACTCGT CCATGGCTCT AACAATGCTG GGAGTGGCAC AGAGGGTCTC 
AAGCCCCCCT TGAGACACGC GCTTAACCTC AGTCAGGAGA AAGTAGAAAT GGAAGACAGT 
GAACTTGATA CTCAGTATTT GCAGAATACA TTTCAAGTTT CAAAGCGTCA GTCATTTGCT 
TTATTTTCAA AACCTAGAAG TCCCCAAAAG GACTGTGCTC ACTCTGTGCC CTCAAAGGAA 
CTGAGTCCAA AGGTGACAGC TAAAGGTAAA CAAAAAGAAC GTCAGGGACA GGAAGAATTT 
GAAATCAGTC ACGTACAAGC AGTTGCGGCC ACAGTGGGCT TACCTGTGCC CTGTCAAGAA 
GTTTCTCCCA TCAGGTCATC TATAAAAACT GACAATAGGA AACCTCTGAC AGAGGGACGA 
TTTGAGAGAC ATACATCATC AACTGAGATG GCGGTGGGAA ATGAGAACAT TCTTCAGAGT 
ACAGTGCACA CAGTTAGCCT GAATAACAGA GGAAATGCTT GTCAAGAAGC CGGCTCGGGC 
AGTATTCATG AAGTATGTTC CACTGGTGAC TCCTTCCCAG GACAACTAGG TAGAAACAGA 
GGGCCTAAGG TGAACACTGT GCCTCCATTA GATAGTATGC AGCCTGGTGT CTGTCAGCAA 
AGTGTTCCTG TAAGTGATAA GTATCTTGAA ATAAAAAAGC AGGAGGGTGA GGCTGTCTGT 
GCAGACTTCT CTCCATGTCT ATTCTCAGAC CATCTTGAGC AATCTATGAG TGGTAAGGTT 
TTTCAGGTTT GCTCTGAGAC ACCTGATGAC CTGCTGGATG ATGTTGAAAT ACAGGGACAT 
ACTAGCTTTG GTGAAGGTGA CATAATGGAG AGATCTGCTG TCTTTAACGG AAGCATCCTG 
AGAAGGGAGT CCAGTAGGAG CCCTAGTCCT GTAACCCATG CATCGAAGTC TCAGAGTCTC 
CACAGAGCGT CTAGGAAATT AGAATCGTCA GAAGAGAGCG ACTCCACTGA GGATGAAGAT 
CTTCCCTGCT TCCAACACTT ACTGAGCAGA ATAAGCAACA CACCTGAGCT TACCAGATGC 
AGCAGTGCTG TGACACAGCG TATGCCAGAG AAAGCGGAGG GGACCCAAGC ACCATGGAAG 
GGTAGCAGCA GTGACTGCAA TAATGAGGTG ATCATGATAG AGGCATCTCA GGAGCATCAG 
TTTAGTGAGG ATCCAAGATG CTCTGGCAGC ATGTTCTCTT CACAGCACAG TGCTGCCCAA 
GGGTCAACTG CAAATGCAAA CTCCCAGGAT TCCAATTTTA TTCCACCTTC CAAACAGAGG 
AGTCACCAGT GTGGGAATGA GGAAGCTTTC CTAAGTGACA AGGAATTGAT TTCAGATAAC 
GAGGAAATGG CAACTTGCCT AGAAGAGGAT AATGACCAAG AAGAGGATAG TATAATCCCA 
GATTCAGAGG CATCCGGATA CGAGAGTGAA ACAAACCTTT CTGAAGACTG CTCGCAGAGT 
GATATTTTAA CCACTCAGCA GCGGGCGACC ATGAAGTATA ACCTGATAAA GCTGCAGCAG 
GAAATGGCTC ACCTGGAAGC TGTGCTGGAG CAGCGTGGGA ACCAGCCTTC TGGCCACTCC 
CCTTCCCTCC TAGCGGACCC TTGTGCCCTG GAAGACCTGC CAGATCTGGA ACCAAACATG 
TCAGGAGCAG CAATTTTAAC TTCAAAGAAC ATTAATGAGA ATCCTGTAAG CCAAAATTTG 
AAGAGCGCTT GTGATGACAA ATTCCAACTA CAACATCTGG AGGGTCCCAC CAGTGGAGAT 
GACGAGTCAG GGATGGGAAG GCCTTCCCCT TTTAAATCTC CGTTGGCAGG CAGTAGGGGC 
TCTGCACATG GCTGCTCTAG GCATCTTCAA AAGAGAAACT CCCCCTCTCA GGAGGAGCTC 
CTCCAGCCTG CTGGATCAGA GGCGTCATCT GAGCCACACA ATTCAACAGG GCAGTCTTGC 
CTGCCAAGGC GAGAGCTAGA AGGAACCCCA TACCTGGGAT CTGGAATCAG CCTTTTCTCT 
AGTAGAGACC CCGAATCTGA GTCCCCTAAA GAGCCAGCCC ACATTGGCAC CACACCAGCT 
TCAACCTCTG CACTGAAAAT ACCCCAAGGT CAAGTTGCTT TCCGGAGTGC AGCTGCTGCT 
GGTGCTGATA AAGCAGTGGT AGGAATTGTG AGCAAGATAA AGCCGGAATT GACATCTTCA 
GAAGAAAGAG CGGATAGAGA CATATCCATG GTGGTGTCAG GCTTGACCCC CAAAGAAGTA 
ATGACCGTGC AAAAGTTTGC TGAAAAATAC CGCCTCACTT TAACTGACGC AATTACTGAG 
GAGACTACAC ATGTAATTAT AAAAACAGAT GCGGAGTTTG TGTGTGAGCG GACACTGAAA 
TATTTTCTGG GCATTGCAGG AGGAAAGTGG ATAGTTAGCT ATTCATGGGT GGTCCGGTCT 
ATCCAAGAAA GAAGACTTCT GAATGTGCAT GAATTTGAAG TCAAAGGAGA TGTTGTGACT 
GGAAGAAATC ACCAAGGTCC AAGGCGATCC AGAGAATCCC GGGAAAAGCT CTTCAAGGGC 
CTACAGGTCT ATTGTTGTGA GCCCTTCACC AACATGCCCA AAGATGAGCT GGAGAGGATG 
CTGCAGCTGT GTGGGGCTTC CGTGGTGAAG GAGCTTCCAT CGCTCACCCA TGACACAGGT 
GCTCATCTAG TTGTGATCGT GCAGCCAAGC GCCTGGACAG AAGACAGCAA CTGCCCAGAT 
ATTGGGCAGC TGTGCAAGGC ACGTCTTGTG ATGTGGGACT GGGTGTTGGA CAGTCTATCC 
AGCTACCGGT GTCGGGATCT GGATGCCTAC CTGGTACAGA ATATCACCTG TGACAGTAGT 
GAGCCACAAG ACTCCAATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532127.1
CDS	203..5506
Translation	MDLSAVQIQEVQNVLHAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNEITKRSLQGSTRFSQLAEELLRIMAAFELDTGMQLTNGFSFSKKRNNSCERLNEEASIIQSVGYRNRVRRLPQVEPGNATLKDSLGVQLSNLGIVRSVKKNRQTQPRKKSVYIELDSDSSEETVTKPGDCSVRDQELLQTAPQEAGDEGKLHSAEEAACEFSEGIRNIEHHQCSDDLNPTENHATERHPEKCQSISISNVCVEPCGTDAHASSLQPETSSLLLIEDRMNAEKAEFCNKSKQPGIAVSQQSRWAASKGTCNDRQVPSTGEKVGPNADSLSDREKWTHPQSLCPENSGATTDVPWITLNSSVQKVNEWFSRTGEMLTSDSASARRHESNAEAAVVLEVSNEVDGGFSSSRKTDLVTPDPHHTLMCKSGRDFSKPVEDNISDKIFGKSYQRKGSRPHLNHVTEIIGTFITEPQITQEQPFTNKLKRKRSTSLQPEDFIKKADSAGVQRTPDNINQGTDLMEPNEQAVSTTSNCQENKIAGSNLQKEKSAHPTESLRKEPASTAGAKSISNSVSDLEVELNVHSSKAPKKNRLRRKSSIRCALPLEPISRNPSPPTCAELQIDSCGSSEETKKNHSNQQPAGHLREPQLIEDTEPAADAKKNEPNEHIRKRRASDAFPEEKLMNKAGLLTSCSSPRKSQGPVNPSPQRTGTEQLETRQMSDSAKELGDRVLGGEPSGKTTDRSEESTSVSLVSDTDYDTQNSVSVLDAHTVRYARTGSAQCMTQFVASENPKELVHGSNNAGSGTEGLKPPLRHALNLSQEKVEMEDSELDTQYLQNTFQVSKRQSFALFSKPRSPQKDCAHSVPSKELSPKVTAKGKQKERQGQEEFEISHVQAVAATVGLPVPCQEVSPIRSSIKTDNRKPLTEGRFERHTSSTEMAVGNENILQSTVHTVSLNNRGNACQEAGSGSIHEVCSTGDSFPGQLGRNRGPKVNTVPPLDSMQPGVCQQSVPVSDKYLEIKKQEGEAVCADFSPCLFSDHLEQSMSGKVFQVCSETPDDLLDDVEIQGHTSFGEGDIMERSAVFNGSILRRESSRSPSPVTHASKSQSLHRASRKLESSEESDSTEDEDLPCFQHLLSRISNTPELTRCSSAVTQRMPEKAEGTQAPWKGSSSDCNNEVIMIEASQEHQFSEDPRCSGSMFSSQHSAAQGSTANANSQDSNFIPPSKQRSHQCGNEEAFLSDKELISDNEEMATCLEEDNDQEEDSIIPDSEASGYESETNLSEDCSQSDILTTQQRATMKYNLIKLQQEMAHLEAVLEQRGNQPSGHSPSLLADPCALEDLPDLEPNMSGAAILTSKNINENPVSQNLKSACDDKFQLQHLEGPTSGDDESGMGRPSPFKSPLAGSRGSAHGCSRHLQKRNSPSQEELLQPAGSEASSEPHNSTGQSCLPRRELEGTPYLGSGISLFSSRDPESESPKEPAHIGTTPASTSALKIPQGQVAFRSAAAAGADKAVVGIVSKIKPELTSSEERADRDISMVVSGLTPKEVMTVQKFAEKYRLTLTDAITEETTHVIIKTDAEFVCERTLKYFLGIAGGKWIVSYSWVVRSIQERRLLNVHEFEVKGDVVTGRNHQGPRRSRESREKLFKGLQVYCCEPFTNMPKDELERMLQLCGASVVKELPSLTHDTGAHLVVIVQPSAWTEDSNCPDIGQLCKARLVMWDWVLDSLSSYRCRDLDAYLVQNITCDSSEPQDSND

Target ORF information:

RefSeq Version	XM_006532064.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus breast cancer 1, early onset (Brca1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532064.3

ORF Insert Sequence:

ATGGATTTAT CTGCCGTCCA AATTCAAGAA GTACAAAATG TCCTTCATGC TATGCAGAAA 
ATCTTAGAGT GTCCGATCTG TTTGGAACTG ATCAAAGAAC CTGTTTCCAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTTAACC AGAAGAAAGG GCCTTCACAA 
TGTCCTTTGT GTAAGAATGA GATAACCAAA AGGAGCCTAC AGGGAAGCAC AAGGTTTAGT 
CAGCTTGCTG AAGAGCTGCT GAGAATAATG GCTGCTTTTG AGCTTGACAC GGGAATGCAG 
CTTACAAATG GTTTTAGTTT TTCAAAAAAG AGAAATAATT CTTGTGAGCG TTTGAATGAG 
GAGGCGTCGA TCATCCAGAG CGTGGGCTAC CGGAACCGTG TCAGAAGGCT TCCCCAGGTC 
GAACCTGGAA ATGCCACCTT GAAGGACAGC CTAGGTGTCC AGCTGTCTAA CCTTGGAATC 
GTGAGATCAG TGAAGAAAAA CAGGCAGACC CAACCTCGAA AGAAATCTGT CTACATTGAA 
CTAGACTCTG ATTCTTCTGA AGAGACAGTA ACTAAGCCAG GTGATTGCAG TGTGAGAGAC 
CAGGAATTGT TACAGACCGC CCCTCAAGAA GCTGGAGATG AAGGCAAGCT GCACTCTGCA 
GAAGAGGCTG CTTGTGAGTT TTCTGAGGGC ATAAGAAACA TTGAACATCA TCAATGCAGT 
GATGATTTAA ACCCTACTGA GAATCATGCA ACTGAAAGGC ATCCAGAAAA ATGTCAGAGT 
ATTTCTATTT CAAATGTGTG TGTGGAGCCA TGTGGCACAG ATGCTCATGC CAGCTCATTA 
CAGCCTGAGA CCAGCAGTTT ATTGCTCATT GAAGACAGAA TGAATGCAGA AAAGGCTGAA 
TTCTGTAATA AAAGCAAACA GCCTGGCATA GCAGTGAGCC AGCAGAGCAG ATGGGCTGCA 
AGTAAAGGAA CATGTAACGA CAGGCAGGTT CCCAGCACTG GGGAAAAGGT AGGTCCAAAC 
GCTGACTCCC TTAGTGATAG AGAGAAGTGG ACTCACCCGC AAAGTCTGTG CCCTGAGAAT 
TCTGGAGCTA CCACCGATGT TCCTTGGATA ACACTAAATA GCAGCGTTCA GAAAGTTAAT 
GAGTGGTTTT CCAGAACTGG TGAAATGTTA ACTTCTGACA GCGCATCTGC CAGGAGGCAC 
GAGTCAAATG CTGAAGCAGC TGTTGTGTTG GAAGTTTCAA ACGAAGTGGA TGGGGGTTTT 
AGTTCTTCAA GGAAAACAGA CTTAGTAACC CCCGACCCCC ATCATACTTT AATGTGTAAA 
AGTGGAAGAG ACTTCTCCAA ACCAGTAGAG GATAATATCA GTGATAAAAT ATTTGGGAAA 
TCCTATCAGA GAAAGGGAAG CCGCCCTCAC CTGAACCATG TGACTGAAAT TATAGGCACA 
TTTATTACAG AACCACAGAT AACACAAGAG CAGCCCTTCA CAAATAAATT AAAACGTAAG 
AGAAGTACAT CCCTTCAACC TGAGGACTTC ATCAAGAAAG CAGATTCAGC AGGTGTTCAA 
AGGACTCCTG ACAACATAAA TCAGGGAACT GACCTAATGG AGCCAAATGA GCAAGCAGTG 
AGTACTACCA GTAACTGTCA GGAGAACAAA ATAGCAGGTA GTAATCTCCA GAAAGAGAAA 
AGCGCTCATC CAACTGAATC ATTGAGAAAG GAACCTGCTT CCACAGCAGG AGCCAAATCT 
ATAAGCAACA GTGTAAGTGA TTTGGAGGTA GAATTAAACG TCCACAGTTC AAAAGCACCT 
AAGAAAAATA GGCTGAGGAG GAAGTCTTCT ATCAGGTGTG CTCTTCCACT TGAACCAATC 
AGTAGAAATC CAAGCCCACC TACTTGTGCT GAGCTTCAAA TCGATAGTTG TGGTAGCAGT 
GAAGAAACAA AGAAAAACCA TTCCAACCAA CAGCCAGCCG GGCACCTTAG AGAGCCTCAA 
CTCATCGAAG ACACTGAACC TGCAGCGGAT GCCAAGAAGA ACGAGCCAAA TGAACACATA 
AGGAAGAGAC GTGCCAGCGA TGCTTTCCCA GAAGAGAAAT TAATGAACAA AGCTGGTTTA 
TTAACTAGCT GTTCAAGTCC TAGAAAATCT CAAGGGCCTG TCAATCCCAG CCCTCAGAGA 
ACAGGAACAG AGCAACTTGA AACACGCCAA ATGTCTGACA GTGCCAAAGA ACTCGGGGAT 
CGGGTCCTAG GAGGAGAGCC CAGTGGCAAA ACCACTGACC GATCTGAGGA GAGCACCAGC 
GTATCCTTGG TATCTGACAC TGACTACGAC ACTCAGAACA GTGTCTCAGT CCTGGACGCT 
CACACTGTCA GATATGCAAG AACAGGATCC GCTCAGTGTA TGACTCAGTT TGTAGCAAGC 
GAAAACCCCA AGGAACTCGT CCATGGCTCT AACAATGCTG GGAGTGGCAC AGAGGGTCTC 
AAGCCCCCCT TGAGACACGC GCTTAACCTC AGTCAGGAGA AAGTAGAAAT GGAAGACAGT 
GAACTTGATA CTCAGTATTT GCAGAATACA TTTCAAGTTT CAAAGCGTCA GTCATTTGCT 
TTATTTTCAA AACCTAGAAG TCCCCAAAAG GACTGTGCTC ACTCTGTGCC CTCAAAGGAA 
CTGAGTCCAA AGGTGACAGC TAAAGGTAAA CAAAAAGAAC GTCAGGGACA GGAAGAATTT 
GAAATCAGTC ACGTACAAGC AGTTGCGGCC ACAGTGGGCT TACCTGTGCC CTGTCAAGAA 
GTTTCTCCCA TCAGGTCATC TATAAAAACT GACAATAGGA AACCTCTGAC AGAGGGACGA 
TTTGAGAGAC ATACATCATC AACTGAGATG GCGGTGGGAA ATGAGAACAT TCTTCAGAGT 
ACAGTGCACA CAGTTAGCCT GAATAACAGA GGAAATGCTT GTCAAGAAGC CGGCTCGGGC 
AGTATTCATG AAGTATGTTC CACTGGTGAC TCCTTCCCAG GACAACTAGG TAGAAACAGA 
GGGCCTAAGG TGAACACTGT GCCTCCATTA GATAGTATGC AGCCTGGTGT CTGTCAGCAA 
AGTGTTCCTG TAAGTGATAA GTATCTTGAA ATAAAAAAGC AGGAGGGTGA GGCTGTCTGT 
GCAGACTTCT CTCCATGTCT ATTCTCAGAC CATCTTGAGC AATCTATGAG TGGTAAGGTT 
TTTCAGGTTT GCTCTGAGAC ACCTGATGAC CTGCTGGATG ATGTTGAAAT ACAGGGACAT 
ACTAGCTTTG GTGAAGGTGA CATAATGGAG AGATCTGCTG TCTTTAACGG AAGCATCCTG 
AGAAGGGAGT CCAGTAGGAG CCCTAGTCCT GTAACCCATG CATCGAAGTC TCAGAGTCTC 
CACAGAGCGT CTAGGAAATT AGAATCGTCA GAAGAGAGCG ACTCCACTGA GGATGAAGAT 
CTTCCCTGCT TCCAACACTT ACTGAGCAGA ATAAGCAACA CACCTGAGCT TACCAGATGC 
AGCAGTGCTG TGACACAGCG TATGCCAGAG AAAGCGGAGG GGACCCAAGC ACCATGGAAG 
GGTAGCAGCA GTGACTGCAA TAATGAGGTG ATCATGATAG AGGCATCTCA GGAGCATCAG 
TTTAGTGAGG ATCCAAGATG CTCTGGCAGC ATGTTCTCTT CACAGCACAG TGCTGCCCAA 
GGGTCAACTG CAAATGCAAA CTCCCAGGAT TCCAATTTTA TTCCACCTTC CAAACAGAGG 
AGTCACCAGT GTGGGAATGA GGAAGCTTTC CTAAGTGACA AGGAATTGAT TTCAGATAAC 
GAGGAAATGG CAACTTGCCT AGAAGAGGAT AATGACCAAG AAGAGGATAG TATAATCCCA 
GATTCAGAGG CATCCGGATA CGAGAGTGAA ACAAACCTTT CTGAAGACTG CTCGCAGAGT 
GATATTTTAA CCACTCAGCA GCGGGCGACC ATGAAGTATA ACCTGATAAA GCTGCAGCAG 
GAAATGGCTC ACCTGGAAGC TGTGCTGGAG CAGCGTGGGA ACCAGCCTTC TGGCCACTCC 
CCTTCCCTCC TAGCGGACCC TTGTGCCCTG GAAGACCTGC CAGATCTGGA ACCAAACATG 
TCAGGAGCAG CAATTTTAAC TTCAAAGAAC ATTAATGAGA ATCCTGTAAG CCAAAATTTG 
AAGAGCGCTT GTGATGACAA ATTCCAACTA CAACATCTGG AGGGTCCCAC CAGTGGAGAT 
GACGAGTCAG GGATGGGAAG GCCTTCCCCT TTTAAATCTC CGTTGGCAGG CAGTAGGGGC 
TCTGCACATG GCTGCTCTAG GCATCTTCAA AAGAGAAACT CCCCCTCTCA GGAGGAGCTC 
CTCCAGCCTG CTGGATCAGA GGCGTCATCT GAGCCACACA ATTCAACAGG GCAGTCTTGC 
CTGCCAAGGC GAGAGCTAGA AGGAACCCCA TACCTGGGAT CTGGAATCAG CCTTTTCTCT 
AGTAGAGACC CCGAATCTGA GTCCCCTAAA GAGCCAGCCC ACATTGGCAC CACACCAGCT 
TCAACCTCTG CACTGAAAAT ACCCCAAGGT CAAGTTGCTT TCCGGAGTGC AGCTGCTGCT 
GGTGCTGATA AAGCAGTGGT AGGAATTGTG AGCAAGATAA AGCCGGAATT GACATCTTCA 
GAAGAAAGAG CGGATAGAGA CATATCCATG GTGGTGTCAG GCTTGACCCC CAAAGAAGTA 
ATGACCGTGC AAAAGTTTGC TGAAAAATAC CGCCTCACTT TAACTGACGC AATTACTGAG 
GAGACTACAC ATGTAATTAT AAAAACAGAT GCGGAGTTTG TGTGTGAGCG GACACTGAAA 
TATTTTCTGG GCATTGCAGG AGGAAAGTGG ATAGTTAGCT ATTCATGGGT GGTCCGGTCT 
ATCCAAGAAA GAAGACTTCT GAATGTGCAT GAATTTGAAG TCAAAGGAGA TGTTGTGACT 
GGAAGAAATC ACCAAGGTCC AAGGCGATCC AGAGAATCCC GGGAAAAGCT CTTCAAGGGC 
CTACAGGTCT ATTGTTGTGA GCCCTTCACC AACATGCCCA AAGATGAGCT GGAGAGGATG 
CTGCAGCTGT GTGGGGCTTC CGTGGTGAAG GAGCTTCCAT CGCTCACCCA TGACACAGGT 
GCTCATCTAG TTGTGATCGT GCAGCCAAGC GCCTGGACAG AAGACAGCAA CTGCCCAGAT 
ATTGGGCAGC TGTGCAAGGC ACGTCTTGTG ATGTGGGACT GGGTGTTGGA CAGTCTATCC 
AGCTACCGGT GTCGGGATCT GGATGCCTAC CTGGTACAGA ATATCACCTG TGACAGTAGT 
GAGCCACAAG ACTCCAATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Brca1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

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