Brca1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Brca1
Entrez Gene ID	497672
Full Name	BRCA1, DNA repair associated
General protein information	Preferred Names BRCA1, DNA repair associated Names breast cancer type 1 susceptibility protein homolog RING-type E3 ubiquitin transferase BRCA1 breast cancer 1, early onset
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q31
Summary	involved in the DNA damage response and DNA repair [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012514.1	NP_036646.1	breast cancer type 1 susceptibility protein homolog
XM_008768092.2	XP_008766314.1	breast cancer type 1 susceptibility protein homolog isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	BRCA1	NP_009231.2
Mus musculus (house mouse)	Brca1	NP_033894.3
Gallus gallus (chicken)	BRCA1	NP_989500.1
	BRCA1	NP_001108421.1
Canis lupus familiaris (dog)	BRCA1	NP_001013434.1
Bos taurus (cattle)	BRCA1	NP_848668.1
Rattus norvegicus (Norway rat)	Brca1	NP_036646.1
Pan troglodytes (chimpanzee)	BRCA1	NP_001038958.1

Gene Ontology
Bibliography

Related articles in PubMed

A BRCA1-Dependent DNA Damage Response in the Regenerating Adult Peripheral Nerve Milieu.
Krishnan A, Purdy K, Chandrasekhar A, Martinez J, Cheng C, Zochodne DW
Molecular neurobiology(2017 Jun)

NPR3 protects cardiomyocytes from apoptosis through inhibition of cytosolic BRCA1 and TNF-?.
Lin D, Chai Y, Izadpanah R, Braun SE, Alt E
Cell cycle (Georgetown, Tex.)15(18)2414-9(2016 Sep)

FancJ (Brip1) loss-of-function allele results in spermatogonial cell depletion during embryogenesis and altered processing of crossover sites during meiotic prophase I in mice.
Sun X, Brie?o-Enr?quez MA, Cornelius A, Modzelewski AJ, Maley TT, Campbell-Peterson KM, Holloway JK, Cohen PE
Chromosoma125(2)237-52(2016 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NPR3 protects cardiomyocytes from apoptosis through inhibition of cytosolic BRCA1 and TNF-alpha, which are regulators of apoptosis.
Title: NPR3 protects cardiomyocytes from apoptosis through inhibition of cytosolic BRCA1 and TNF-?.

Promoter regions of Brca1 are modified by soy estrogens in prostate cancer cells.
Title: Soy phytoestrogens modify DNA methylation of GSTP1, RASSF1A, EPH2 and BRCA1 promoter in prostate cancer cells.

Akt prevents the translocation of BRCA1 to DNA damage foci and, thereby, inhibits the activation of Chk1 following DNA damage
Title: Akt activation emulates Chk1 inhibition and Bcl2 overexpression and abrogates G2 cell cycle checkpoint by inhibiting BRCA1 foci.

Pubertal physical activity up-regulates tumor suppressor genes BRCA1, p53 and ER-beta, and reduced ER-alpha/ER-beta ratio in the mammary gland
Title: Prepubertal physical activity up-regulates estrogen receptor beta, BRCA1 and p53 mRNA expression in the rat mammary gland.

Data show that knockdown of BRCA1 inhibited nuclear translocation of the vitamin D receptor and the ability of 1alpha,25(OH)(2)D(3) to induce the VDR.
Title: Prolactin blocks nuclear translocation of VDR by regulating its interaction with BRCA1 in osteosarcoma cells.

The following Brca1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Brca1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00156	NM_012514.1 Latest version!	Rattus norvegicus BRCA1, DNA repair associated (Brca1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa48994	XM_008768092.2 Latest version!	Rattus norvegicus BRCA1, DNA repair associated (Brca1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Brca1 ( NM_012514.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036646.1 Rattus norvegicus BRCA1, DNA repair associated (Brca1), mRNA.

Price & Availability↑

CloneID	ORa00156
Clone ID Related Accession (Same CDS sequence)	NM_012514.1
Accession Version	NM_012514.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	breast cancer type 1 susceptibility protein homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF036760.1. On Jan 12, 2005 this sequence version replaced XM_346407.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF036760.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGATTTAT CTGCTGTTCG AATTCAAGAA GTACAAAATG TCCTTCATGC TATGCAGAAA 
ATCTTGGAGT GTCCAATCTG TTTGGAACTG ATCAAAGAAC CGGTTTCCAC ACAGTGCGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTCCTTAACC AGAAGAAAGG ACCTTCCCAG 
TGTCCTTTGT GTAAGAATGA GATAACCAAA AGGAGCCTAC AAGGAAGTGC AAGGTTTAGT 
CAACTTGTTG AAGAGCTGCT GAAAATCATT GATGCTTTTG AGCTTGACAC CGGAATGCAG 
TGTGCAAATG GTTTTAGTTT TTCAAAAAAG AAAAATAGTT CTTCTGAGCT TCTGAATGAG 
GACGCTTCTA TCATCCAGAG TGTGGGCTAC CGAAACCGCG TCAAAAAACT TCAACAGATT 
GAGTCTGGAA GTGCCACCTT GAAGGACAGT CTCAGTGTCC AGTTGTCTAA CCTTGGGATT 
GTGAGATCAA TGAAGAAAAA TAGGCAGACA CAACCTCAAA ACAAATCTGT CTACATTGCT 
TTAGAATCTG ATTCTTCTGA GGAGAGAGTA AATGCACCCG ATGGCTGCAG TGTGAGAGAT 
CAGGAATTGT TTCAGATCGC CCCTGGAGGA GCTGGAGATG AAGGCAAGTT GAACTCTGCA 
AAAAAGGCTG CTTGTGACTT TTCTGAGGGC ATAAGAAACA TTGAACATCA TCAATGCAGT 
GATAAAGATT TGAACCCCAC TGAGAATCAT GCAACTGAAA GGCATCCAGA AAAATGTCCG 
CGTATTTCTG TTGCAAACGT GCACGTGGAG CCGTGTGGCA CAGATGCTCG TGCCAGCTCA 
TTACAGCGTG GGACCCGCAG TTTATTGTTC ACTGAGGACA GACTGGATGC AGAAAAGGCT 
GAATTCTGTG ATAGAAGCAA ACAGTCTGGC GCAGCAGTGA GCCAGCAGAG CAGATGGGCT 
GACAGTAAAG AAACATGTAA TGGCAGGCCG GTTCCCCGCA CTGAGGGAAA GGCAGATCCA 
AATGTGGATT CCCTCTGTGG TAGAAAGCAG TGGAATCATC CGAAAAGCCT GTGCCCTGAG 
AATTCTGGAG CTACCACTGA CGTTCCTTGG ATAACACTGA ATAGCAGCAT TCAGAAAGTG 
AATGAGTGGT TTTCCAGAAC TGGTGAAATG TTAACTTCTG ACAATGCATC TGACAGGAGG 
CCTGCGTCAA ATGCAGAAGC TGCTGTTGTG TTAGAAGTTT CAAATGAAGT GGATGGATGT 
TTCAGTTCTT CAAAGAAAAT AGACTTAGTT GCCCCTGATC CCGATAATGC TGTAATGTGT 
ACAAGTGGAA GAGACTTCTC CAAGCCAGTA GAGAATATTA TCAACGATAA AATATTTGGG 
AAAACCTATC AGAGAAAGGG AAGCCGCCCT CACTTGAACC ATGTGACTGA AATTATAGGC 
ACATTTACTA CAGAACCACA GATTATACAA GAGCAGCCCT TCACAAATAA ATTAAAACGC 
AAAAGAAGTA CATGCCTTCA TCCTGAGGAC TTTATCAAGA AAGCAGATTT AACAGTTGTT 
CAAAGGATTT CTGAAAATTT AAATCAGGGA ACTGACCAAA TGGAGCCAAA TGACCAAGCA 
ATGAGTATTA CCAGTAACGG TCAGGAGAAC AGAGCAACAG GTAATGATCT TCAGAGAGGG 
AGAAATGCTC ATCCAATAGA ATCATTGAGA AAGGAACCTG CTTTCACAGC TAAAGCCAAA 
TCTATAAGCA ACAGTATAAG TGATTTGGAG GTAGAATTAA ATGTTCACAG TTCAAAAGCA 
CCTAAGAAAA ATAGGCTGAG GAGGAAGTCT ACCAGGTGTG TTCTTCCACT CGAACCAATC 
AGTAGAAATC CGAGCCCACC TACTTGTGCT GAACTTCAGA TCGAGAGTTG TGGTAGCAGT 
GAAGAAACAA AGAAAAACAA TTCCAACCAA ACCCCAGCCG GGCACATTAG AGAGCCTCAA 
CTCATCGAAG ACACAGAACC CGCAGCTGAT GCCAAGAAGA ACGAGCCAAA TGAACACATA 
AGGAAGAGAA GTGCCAGTGA TGCGTTCCCA GAAGAGAAAT TAATGAACAA AGCTGGTTTA 
TTAACTAGCT GTTCAAGTCC TAGAAAGCCT CAAGGACCTG TCAATCCTAG CCCTGAGAGA 
AAAGGAATAG AGCAACTTGA AATGTGCCAG ATGCCTGATA ATAACAAAGA ACTCGGGGAT 
TTGGTCCTGG GAGGAGAGCC CAGTGGGAAA CCTACTGAAC CATCTGAGGA GAGCACCAGT 
GTGTCCTTGG TACCCGACAC AGACTACGAC ACCCAGAACA GTGTCTCAAT ACTGGAAGCG 
AACACTGTCA GATATGCAAG AACAGGATCA GTTCAGTGTA TGACTCAGTT TGTCGCAAGT 
GAAAACCCCA AGGAACTTGT CCATGGTTCT AACAATGCTG GAAGTGGCTC GGAGTGCTTC 
AAGCACCCAT TGAGACATGA ACTTAACCAC AATCAAGAGA CAATAGAAAT GGAAGACAGT 
GAACTTGATA CTCAGTATTT GCAGAATACA TTTCAAGTTT CAAAGCGTCA GTCATTTGCT 
TTATTTTCAA AACTTAGAAG TCCCCAAAAG GACTGTACTC TGGTAGGTGC CCGCTCTGTG 
CCCTCAAGGG AACCAAGTCC AAAGGTGACT TCTAGAGGTG AACAAAAAGA ACGTCAGGGA 
CAAGAAGAGT CTGAAATCAG TCATGTACAG GCAGTCACAG TCACAGTAGG CTTACCTGTG 
CCCTGTCAGG AAGGTAAGCC AGGTGCTGTT ACAATGTGTG CTGATGTTTC TAGGCTTTGT 
CCGTCATCTC ATTATAGAAG CTGTGAGAAT GGACTCAACA CCACAGATAA ATCTGGAATT 
TCACAAAACT CACATTTTAG ACAATCAGTT TCTCCCCTCA GGTCATCTAT AAAAACTGAC 
AATAGAAAAA CTCTGACAGA GGGNCGATTT GAGAAACATA CTGAAAGGGG RATGGGAAAT 
GAGACTGCTG TTCAAAGTAC AATACACACA ATTAGTCTAA ATAACAGAGG AGATGCTTGT 
CTAGAAGCCA GCTCAGGCAG TGTTATTGAA GTACATTCCA CTGGTGAAAA CGTCCAGGGG 
CAACTAGATA GAAACAGAGG GCCTAAGGTA AACACCGTGT CTCTATTAGA TAGTACACAG 
CCTGGTGTCT CTAAGCAGAG TGCTCCTGTA AGTGATAAGT ATCTTGAAAT AAAGCAGGAG 
AGTAAGGCTG TCAGTGCAGA CTTCTCTCCA TGTCTGTTCT CAGATCATCT TGAAAAACCT 
ATGAGAAGTG ATAAGACTTT TCAGGTTTGC TCTGAGACAC CTGATGACCT GTTGGATGAT 
GTTGAAATAC AGGAAAATGC TAGCTTCGGT GAAGGTGGCA TAACGGAAAA GTCTGCTATT 
TTTAATGGAA GTGTCCTGAG AAGAGAGTCC AGTAGGAGCC CTAGCCCTGT AACCCATGCA 
TCGAAGTCGC GGAGTCTCCA CAGAGGGTCT AGGAAATTAG AATTCTCAGA AGAGAGCGAC 
TCCACTGAGG ATGAAGACCT TCCCTGCTTC CAACACTTGC TGAGCAGAGT AAGCAGCACA 
CCCGAACTTA CCAGATGCAG CAGTGTGGTG ACACAGCGTG TGCCAGAGAA AGCGAAGGGG 
ACCCAAGCAC CCCGGAAGAG TAGCATCAGT GACTGCAATA ATGAGGTGAT CTTGGGAGAG 
GCATCTCAGG AATATCAGTT TAGTGAGGAT GCAAAATGCT CTGGCAGCAT GTTCTCCTCA 
CAGCACAGTG CTGCCCTAGG TTCNCCTGCA AATGCACTCT CCCAGGATCC CGATTTTAAT 
CCACCTTCCA AACAAAGGCG TCACCAGGCT GAGAATGAGG AAGCCTTTCT AAGTGATAAG 
GAATTGATTT CAGATCATGA GGACATGGCA GCTTGCCTGG AAGAGGCTAG CGATCAAGAA 
GAGGACAGTA TAATCCCGGA CTCAGTGGCG TCTGGATATG AGAGTGAAGC AAACCTCTCT 
GAAGACTGCT CACAGAGTGA TATTCTAACC ACTCAGCAGA GGGCGACCAT GAAGGATAAC 
CTGATAAAGC TCCAGCAGGA AATGGCTCAG CTGGAAGCTG TGCTAGAGCA GCATGGGAGC 
CAGCCGTCTG GCCACCCCCC TTGCCTCCCA GCTGACCCTT GTGCCCTAGA AGACCTGCCA 
GATCCAGAAC AAAACAGATC AGGAACAGCA ATTTTAACTT CAAAGAACAT TAATGAGAAT 
CCTGTAAGCC AGAATCCAAA GCGCGCCTGT GATGACAAAT CCCAACCACA ACCTCCGGAC 
GGTCTCCCCA GTGGAGATAA GGAGTCAGGA ATGAGAAGGC CTTCCCCTTT TAAATCTCCA 
CTGACCAGCA GTAGGTGCTC TGCACGAGGC CACTCCAGGA GTCTTCAGAA TAGAAACTCC 
ACSTCGCAAG AGGAGCTCCT TCAGCCTGCT GRATTGGAGA AGTCATGTGA ACCACACAAC 
TTAACAGGGC GGTCTTGCTT GCCAAGGCAA GATCTCGAAG GAACCCCATA CCCGGAATCT 
GGAATCAGGC TAGTCTCCAG CAGAGACCCT GATTCTGAAT CCCCTAAAGT GTCAGCCCTT 
GTTTGCACTG CACCAGCTTC AACCTCTGCA CTGAAAATAT CCCAAGGTCA AGTTGCTGGG 
TCTTGCAGGA GTCCAGCTGC TGGTGGTGCT GATACTGCAG TGGTAGAAAT TGTGAGCAAG 
ATAAAGCCGG AAGTGACGTC TCCAAAAGAA CGAGCGGAGA GAGATATATC CATGGTGGTG 
TCAGGCTTGA CCCCTAAAGA AGTAATGATT GTCCAAAAGT TTGCTGAAAA ATACCGCCTT 
GCTTTAACTG ATGTAATTAC TGAGGAAACT ACGCATGTAA TTATAAAAAC AGATGCTGAG 
TTTGTGTGTG AACGGACACT GAAATATTTT CTGGGCATTG CAGGAGGAAA GTGGATAGTT 
AGCTATTCTT GGGTGATCAA GTCTATCCAA GAAAGAAAAC TTCTGAGTGT GCATGAATTT 
GAAGTCAAAG GAGACGTTGT GACTGGAAGC AACCACCAAG GTCCAAGGCG ATCCAGAGAA 
TCCCAGGAAA AGCTCTTTGA AGGCCTACAG ATCTATTGTT GTGAGCCCTT CACCAACATG 
CCCAAAGATG AGCTGGAGAG GATGCTGCAG CTGTGTGGGG CTTCTGTGGT GAAGGAGCTT 
CCATTGCTCA CCCGTGACAC AGGTGCTCAT CCAATTGTGC TCGTGCAGCC AAGTGCCTGG 
ACAGAAGACA ACGACTGCCC TGATATTGGG CAGCTGTGCA AGGGACGTCT AGTGATGTGG 
GACTGGGTGT TGGACAGTAT ATCCGTCTAC CGGTGTCGGG ATCTGGATGC CTACCTGGTA 
CAGAATATCA CCTGTGGCCG TGATGGTAGC GAGCCACAAG ACTCCAATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036646.1
CDS	118..5571
Translation	MDLSAVRIQEVQNVLHAMQKILECPICLELIKEPVSTQCDHIFCKFCMLKLLNQKKGPSQCPLCKNEITKRSLQGSARFSQLVEELLKIIDAFELDTGMQCANGFSFSKKKNSSSELLNEDASIIQSVGYRNRVKKLQQIESGSATLKDSLSVQLSNLGIVRSMKKNRQTQPQNKSVYIALESDSSEERVNAPDGCSVRDQELFQIAPGGAGDEGKLNSAKKAACDFSEGIRNIEHHQCSDKDLNPTENHATERHPEKCPRISVANVHVEPCGTDARASSLQRGTRSLLFTEDRLDAEKAEFCDRSKQSGAAVSQQSRWADSKETCNGRPVPRTEGKADPNVDSLCGRKQWNHPKSLCPENSGATTDVPWITLNSSIQKVNEWFSRTGEMLTSDNASDRRPASNAEAAVVLEVSNEVDGCFSSSKKIDLVAPDPDNAVMCTSGRDFSKPVENIINDKIFGKTYQRKGSRPHLNHVTEIIGTFTTEPQIIQEQPFTNKLKRKRSTCLHPEDFIKKADLTVVQRISENLNQGTDQMEPNDQAMSITSNGQENRATGNDLQRGRNAHPIESLRKEPAFTAKAKSISNSISDLEVELNVHSSKAPKKNRLRRKSTRCVLPLEPISRNPSPPTCAELQIESCGSSEETKKNNSNQTPAGHIREPQLIEDTEPAADAKKNEPNEHIRKRSASDAFPEEKLMNKAGLLTSCSSPRKPQGPVNPSPERKGIEQLEMCQMPDNNKELGDLVLGGEPSGKPTEPSEESTSVSLVPDTDYDTQNSVSILEANTVRYARTGSVQCMTQFVASENPKELVHGSNNAGSGSECFKHPLRHELNHNQETIEMEDSELDTQYLQNTFQVSKRQSFALFSKLRSPQKDCTLVGARSVPSREPSPKVTSRGEQKERQGQEESEISHVQAVTVTVGLPVPCQEGKPGAVTMCADVSRLCPSSHYRSCENGLNTTDKSGISQNSHFRQSVSPLRSSIKTDNRKTLTEnullRFEKHTERnullMGNETAVQSTIHTISLNNRGDACLEASSGSVIEVHSTGENVQGQLDRNRGPKVNTVSLLDSTQPGVSKQSAPVSDKYLEIKQESKAVSADFSPCLFSDHLEKPMRSDKTFQVCSETPDDLLDDVEIQENASFGEGGITEKSAIFNGSVLRRESSRSPSPVTHASKSRSLHRGSRKLEFSEESDSTEDEDLPCFQHLLSRVSSTPELTRCSSVVTQRVPEKAKGTQAPRKSSISDCNNEVILGEASQEYQFSEDAKCSGSMFSSQHSAALGnullPANALSQDPDFNPPSKQRRHQAENEEAFLSDKELISDHEDMAACLEEASDQEEDSIIPDSVASGYESEANLSEDCSQSDILTTQQRATMKDNLIKLQQEMAQLEAVLEQHGSQPSGHPPCLPADPCALEDLPDPEQNRSGTAILTSKNINENPVSQNPKRACDDKSQPQPPDGLPSGDKESGMRRPSPFKSPLTSSRCSARGHSRSLQNRNSnullSQEELLQPAnullLEKSCEPHNLTGRSCLPRQDLEGTPYPESGIRLVSSRDPDSESPKVSALVCTAPASTSALKISQGQVAGSCRSPAAGGADTAVVEIVSKIKPEVTSPKERAERDISMVVSGLTPKEVMIVQKFAEKYRLALTDVITEETTHVIIKTDAEFVCERTLKYFLGIAGGKWIVSYSWVIKSIQERKLLSVHEFEVKGDVVTGSNHQGPRRSRESQEKLFEGLQIYCCEPFTNMPKDELERMLQLCGASVVKELPLLTRDTGAHPIVLVQPSAWTEDNDCPDIGQLCKGRLVMWDWVLDSISVYRCRDLDAYLVQNITCGRDGSEPQDSND

Target ORF information:

RefSeq Version	NM_012514.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus BRCA1, DNA repair associated (Brca1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012514.1

ORF Insert Sequence:

ATGGATTTAT CTGCTGTTCG AATTCAAGAA GTACAAAATG TCCTTCATGC TATGCAGAAA 
ATCTTGGAGT GTCCAATCTG TTTGGAACTG ATCAAAGAAC CGGTTTCCAC ACAGTGCGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTCCTTAACC AGAAGAAAGG ACCTTCCCAG 
TGTCCTTTGT GTAAGAATGA GATAACCAAA AGGAGCCTAC AAGGAAGTGC AAGGTTTAGT 
CAACTTGTTG AAGAGCTGCT GAAAATCATT GATGCTTTTG AGCTTGACAC CGGAATGCAG 
TGTGCAAATG GTTTTAGTTT TTCAAAAAAG AAAAATAGTT CTTCTGAGCT TCTGAATGAG 
GACGCTTCTA TCATCCAGAG TGTGGGCTAC CGAAACCGCG TCAAAAAACT TCAACAGATT 
GAGTCTGGAA GTGCCACCTT GAAGGACAGT CTCAGTGTCC AGTTGTCTAA CCTTGGGATT 
GTGAGATCAA TGAAGAAAAA TAGGCAGACA CAACCTCAAA ACAAATCTGT CTACATTGCT 
TTAGAATCTG ATTCTTCTGA GGAGAGAGTA AATGCACCCG ATGGCTGCAG TGTGAGAGAT 
CAGGAATTGT TTCAGATCGC CCCTGGAGGA GCTGGAGATG AAGGCAAGTT GAACTCTGCA 
AAAAAGGCTG CTTGTGACTT TTCTGAGGGC ATAAGAAACA TTGAACATCA TCAATGCAGT 
GATAAAGATT TGAACCCCAC TGAGAATCAT GCAACTGAAA GGCATCCAGA AAAATGTCCG 
CGTATTTCTG TTGCAAACGT GCACGTGGAG CCGTGTGGCA CAGATGCTCG TGCCAGCTCA 
TTACAGCGTG GGACCCGCAG TTTATTGTTC ACTGAGGACA GACTGGATGC AGAAAAGGCT 
GAATTCTGTG ATAGAAGCAA ACAGTCTGGC GCAGCAGTGA GCCAGCAGAG CAGATGGGCT 
GACAGTAAAG AAACATGTAA TGGCAGGCCG GTTCCCCGCA CTGAGGGAAA GGCAGATCCA 
AATGTGGATT CCCTCTGTGG TAGAAAGCAG TGGAATCATC CGAAAAGCCT GTGCCCTGAG 
AATTCTGGAG CTACCACTGA CGTTCCTTGG ATAACACTGA ATAGCAGCAT TCAGAAAGTG 
AATGAGTGGT TTTCCAGAAC TGGTGAAATG TTAACTTCTG ACAATGCATC TGACAGGAGG 
CCTGCGTCAA ATGCAGAAGC TGCTGTTGTG TTAGAAGTTT CAAATGAAGT GGATGGATGT 
TTCAGTTCTT CAAAGAAAAT AGACTTAGTT GCCCCTGATC CCGATAATGC TGTAATGTGT 
ACAAGTGGAA GAGACTTCTC CAAGCCAGTA GAGAATATTA TCAACGATAA AATATTTGGG 
AAAACCTATC AGAGAAAGGG AAGCCGCCCT CACTTGAACC ATGTGACTGA AATTATAGGC 
ACATTTACTA CAGAACCACA GATTATACAA GAGCAGCCCT TCACAAATAA ATTAAAACGC 
AAAAGAAGTA CATGCCTTCA TCCTGAGGAC TTTATCAAGA AAGCAGATTT AACAGTTGTT 
CAAAGGATTT CTGAAAATTT AAATCAGGGA ACTGACCAAA TGGAGCCAAA TGACCAAGCA 
ATGAGTATTA CCAGTAACGG TCAGGAGAAC AGAGCAACAG GTAATGATCT TCAGAGAGGG 
AGAAATGCTC ATCCAATAGA ATCATTGAGA AAGGAACCTG CTTTCACAGC TAAAGCCAAA 
TCTATAAGCA ACAGTATAAG TGATTTGGAG GTAGAATTAA ATGTTCACAG TTCAAAAGCA 
CCTAAGAAAA ATAGGCTGAG GAGGAAGTCT ACCAGGTGTG TTCTTCCACT CGAACCAATC 
AGTAGAAATC CGAGCCCACC TACTTGTGCT GAACTTCAGA TCGAGAGTTG TGGTAGCAGT 
GAAGAAACAA AGAAAAACAA TTCCAACCAA ACCCCAGCCG GGCACATTAG AGAGCCTCAA 
CTCATCGAAG ACACAGAACC CGCAGCTGAT GCCAAGAAGA ACGAGCCAAA TGAACACATA 
AGGAAGAGAA GTGCCAGTGA TGCGTTCCCA GAAGAGAAAT TAATGAACAA AGCTGGTTTA 
TTAACTAGCT GTTCAAGTCC TAGAAAGCCT CAAGGACCTG TCAATCCTAG CCCTGAGAGA 
AAAGGAATAG AGCAACTTGA AATGTGCCAG ATGCCTGATA ATAACAAAGA ACTCGGGGAT 
TTGGTCCTGG GAGGAGAGCC CAGTGGGAAA CCTACTGAAC CATCTGAGGA GAGCACCAGT 
GTGTCCTTGG TACCCGACAC AGACTACGAC ACCCAGAACA GTGTCTCAAT ACTGGAAGCG 
AACACTGTCA GATATGCAAG AACAGGATCA GTTCAGTGTA TGACTCAGTT TGTCGCAAGT 
GAAAACCCCA AGGAACTTGT CCATGGTTCT AACAATGCTG GAAGTGGCTC GGAGTGCTTC 
AAGCACCCAT TGAGACATGA ACTTAACCAC AATCAAGAGA CAATAGAAAT GGAAGACAGT 
GAACTTGATA CTCAGTATTT GCAGAATACA TTTCAAGTTT CAAAGCGTCA GTCATTTGCT 
TTATTTTCAA AACTTAGAAG TCCCCAAAAG GACTGTACTC TGGTAGGTGC CCGCTCTGTG 
CCCTCAAGGG AACCAAGTCC AAAGGTGACT TCTAGAGGTG AACAAAAAGA ACGTCAGGGA 
CAAGAAGAGT CTGAAATCAG TCATGTACAG GCAGTCACAG TCACAGTAGG CTTACCTGTG 
CCCTGTCAGG AAGGTAAGCC AGGTGCTGTT ACAATGTGTG CTGATGTTTC TAGGCTTTGT 
CCGTCATCTC ATTATAGAAG CTGTGAGAAT GGACTCAACA CCACAGATAA ATCTGGAATT 
TCACAAAACT CACATTTTAG ACAATCAGTT TCTCCCCTCA GGTCATCTAT AAAAACTGAC 
AATAGAAAAA CTCTGACAGA GGGNCGATTT GAGAAACATA CTGAAAGGGG RATGGGAAAT 
GAGACTGCTG TTCAAAGTAC AATACACACA ATTAGTCTAA ATAACAGAGG AGATGCTTGT 
CTAGAAGCCA GCTCAGGCAG TGTTATTGAA GTACATTCCA CTGGTGAAAA CGTCCAGGGG 
CAACTAGATA GAAACAGAGG GCCTAAGGTA AACACCGTGT CTCTATTAGA TAGTACACAG 
CCTGGTGTCT CTAAGCAGAG TGCTCCTGTA AGTGATAAGT ATCTTGAAAT AAAGCAGGAG 
AGTAAGGCTG TCAGTGCAGA CTTCTCTCCA TGTCTGTTCT CAGATCATCT TGAAAAACCT 
ATGAGAAGTG ATAAGACTTT TCAGGTTTGC TCTGAGACAC CTGATGACCT GTTGGATGAT 
GTTGAAATAC AGGAAAATGC TAGCTTCGGT GAAGGTGGCA TAACGGAAAA GTCTGCTATT 
TTTAATGGAA GTGTCCTGAG AAGAGAGTCC AGTAGGAGCC CTAGCCCTGT AACCCATGCA 
TCGAAGTCGC GGAGTCTCCA CAGAGGGTCT AGGAAATTAG AATTCTCAGA AGAGAGCGAC 
TCCACTGAGG ATGAAGACCT TCCCTGCTTC CAACACTTGC TGAGCAGAGT AAGCAGCACA 
CCCGAACTTA CCAGATGCAG CAGTGTGGTG ACACAGCGTG TGCCAGAGAA AGCGAAGGGG 
ACCCAAGCAC CCCGGAAGAG TAGCATCAGT GACTGCAATA ATGAGGTGAT CTTGGGAGAG 
GCATCTCAGG AATATCAGTT TAGTGAGGAT GCAAAATGCT CTGGCAGCAT GTTCTCCTCA 
CAGCACAGTG CTGCCCTAGG TTCNCCTGCA AATGCACTCT CCCAGGATCC CGATTTTAAT 
CCACCTTCCA AACAAAGGCG TCACCAGGCT GAGAATGAGG AAGCCTTTCT AAGTGATAAG 
GAATTGATTT CAGATCATGA GGACATGGCA GCTTGCCTGG AAGAGGCTAG CGATCAAGAA 
GAGGACAGTA TAATCCCGGA CTCAGTGGCG TCTGGATATG AGAGTGAAGC AAACCTCTCT 
GAAGACTGCT CACAGAGTGA TATTCTAACC ACTCAGCAGA GGGCGACCAT GAAGGATAAC 
CTGATAAAGC TCCAGCAGGA AATGGCTCAG CTGGAAGCTG TGCTAGAGCA GCATGGGAGC 
CAGCCGTCTG GCCACCCCCC TTGCCTCCCA GCTGACCCTT GTGCCCTAGA AGACCTGCCA 
GATCCAGAAC AAAACAGATC AGGAACAGCA ATTTTAACTT CAAAGAACAT TAATGAGAAT 
CCTGTAAGCC AGAATCCAAA GCGCGCCTGT GATGACAAAT CCCAACCACA ACCTCCGGAC 
GGTCTCCCCA GTGGAGATAA GGAGTCAGGA ATGAGAAGGC CTTCCCCTTT TAAATCTCCA 
CTGACCAGCA GTAGGTGCTC TGCACGAGGC CACTCCAGGA GTCTTCAGAA TAGAAACTCC 
ACSTCGCAAG AGGAGCTCCT TCAGCCTGCT GRATTGGAGA AGTCATGTGA ACCACACAAC 
TTAACAGGGC GGTCTTGCTT GCCAAGGCAA GATCTCGAAG GAACCCCATA CCCGGAATCT 
GGAATCAGGC TAGTCTCCAG CAGAGACCCT GATTCTGAAT CCCCTAAAGT GTCAGCCCTT 
GTTTGCACTG CACCAGCTTC AACCTCTGCA CTGAAAATAT CCCAAGGTCA AGTTGCTGGG 
TCTTGCAGGA GTCCAGCTGC TGGTGGTGCT GATACTGCAG TGGTAGAAAT TGTGAGCAAG 
ATAAAGCCGG AAGTGACGTC TCCAAAAGAA CGAGCGGAGA GAGATATATC CATGGTGGTG 
TCAGGCTTGA CCCCTAAAGA AGTAATGATT GTCCAAAAGT TTGCTGAAAA ATACCGCCTT 
GCTTTAACTG ATGTAATTAC TGAGGAAACT ACGCATGTAA TTATAAAAAC AGATGCTGAG 
TTTGTGTGTG AACGGACACT GAAATATTTT CTGGGCATTG CAGGAGGAAA GTGGATAGTT 
AGCTATTCTT GGGTGATCAA GTCTATCCAA GAAAGAAAAC TTCTGAGTGT GCATGAATTT 
GAAGTCAAAG GAGACGTTGT GACTGGAAGC AACCACCAAG GTCCAAGGCG ATCCAGAGAA 
TCCCAGGAAA AGCTCTTTGA AGGCCTACAG ATCTATTGTT GTGAGCCCTT CACCAACATG 
CCCAAAGATG AGCTGGAGAG GATGCTGCAG CTGTGTGGGG CTTCTGTGGT GAAGGAGCTT 
CCATTGCTCA CCCGTGACAC AGGTGCTCAT CCAATTGTGC TCGTGCAGCC AAGTGCCTGG 
ACAGAAGACA ACGACTGCCC TGATATTGGG CAGCTGTGCA AGGGACGTCT AGTGATGTGG 
GACTGGGTGT TGGACAGTAT ATCCGTCTAC CGGTGTCGGG ATCTGGATGC CTACCTGGTA 
CAGAATATCA CCTGTGGCCG TGATGGTAGC GAGCCACAAG ACTCCAATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brca1 ( XM_008768092.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766314.1 Rattus norvegicus BRCA1, DNA repair associated (Brca1), transcript variant X1, mRNA.

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CloneID	ORa48994
Clone ID Related Accession (Same CDS sequence)	XM_008768092.2
Accession Version	XM_008768092.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	breast cancer type 1 susceptibility protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768092.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAT CTGCTGTTCG AATTCAAGAA GTACAAAATG TCCTTCATGC TATGCAGAAA 
ATCTTGGAGT GTCCAATCTG TTTGGAACTG ATCAAAGAAC CGGTTTCCAC AAAGTGCGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTCCTTAACC AGAAGAAAGG ACCTTCCCAG 
TGTCCTTTGT GTAAGAATGA GATAACCAAA AGGAGCCTAC AAGGAAGTGC AAGGTTTAGT 
CAACTTGTTG AAGAGCTGCT GAAAATCATT GATGCTTTTG AGCTTGACAC CGGAATGCAG 
TGTGCAAATG GTTTTAGTTT TTCAAAAAAG AAAAATAGTT CTTCTGAGCT TCTGAATGAG 
GACGCTTCTA TCATCCAGAG TGTGGGCTAC CGAAACCGCG TCAAAAAACT TCGACAGATT 
GAGTCTGGAA GTGCCACCTT GAAGGACAGT CTCAGTGTCC AGTTGTCTAA CCTTGGGATT 
GTGAGATCAA TGAAGAAAAA TAGGCAGACA CAACCTCAAA ACAAATCTGT CTACATTGCT 
TTAGAATCTG ATTCTTCTGA GGAGAGAGTA AATGCACCCG ATGGCTGCAG TGTGAGAGAT 
CAGGAATTGT TTCAGATCGC CCCTGGAGGA GCTGGAGATG AAGGCAAGTT GAACTCTGCA 
AAAAAGGCTG CTTGTGACTT TTCTGAGGGC ATAAGAAACA TTGAACATCA TCAATGCAGT 
GATAAAGATT TGAACCCCAC TGAGAATCAT GCAACTGAAA GGCATCCAGA AAAATGTCCG 
CGTATTTCTG TTGCAAACGT GCACGTGGAG CCGTGTGGCA CAGATGCTCG TGCCAGCTCA 
TTACAGCGTG GGACCCGCAG TTTATTGTTC ACTGAGGACA GACTGGATGC AGAAAAGGCT 
GAATTCTGTG ATAGAAGCAA ACAGTCTGGC GCAGCAGTGA GCCAGCAGAG CAGATGGGCT 
GACAGTAAAG AAACATGTAA TGGCAGGCCG GTTCCCCGCA CTGAGGGAAA GGCAGATCCA 
AATGTGGATT CCCTCTGTGG TAGAAAGCAG TGGAATCATC CGAAAAGCCT GTGCCCTGAG 
AATTCTGGAG CTACCACTGA CGTTCCTTGG ATAACACTGA ATAGCAGCAT TCAGAAAGTG 
AATGAGTGGT TTTCCAGAAC TGGTGAAATG TTAACTTCTG ACAATGCATC TGACAGGAGG 
CCTGCGTCAA ATGCAGAAGC TGCTGTTGTG TTAGAAGTTT CAAATGAAGT GGATGGATGT 
TTCAGTTCTT CAAAGAAAAT AGACTTAGTT GCCCCTGATT CCGATAATGC TGTAATGTGT 
ACAAGTGGAA GAGACTTCTC CAAGCCAGTA GAGAATATTA TCAACGATAA AATATTTGGG 
AAAACCTATC AGAGAAAGGG AAGCCGCCCT CACTTGAACC ATGTGACTGA AATTATAGGC 
ACATTTACTA CAGAACCACA GATTATACAA GAGCAGCCCT TCACAAATAA ATTAAAACGC 
AAAAGAAGTA CATGCCTTCA TCCTGAGGAC TTTATCAAGA AAGCAGATTT AACAGTTGTT 
CAAAGGATTT CTGAAAATTT AAATCAGGGA ACTGACCAAA TGGAGCCAAA TGACCAAGCA 
ATGAGTATTA CCAGTAACGG TCAGGAGAAC AGAGCAACAG GTAATGATCT TCAGAGAGGG 
AGAAATGCTC ATCCAATAGA ATCATTGAGA AAGGAACCTG CTTTCACAGC TAAAGCCAAA 
TCTATAAGCA ACAGTATAAG TGATTTGGAG GTAGAATTAA ATGTTCACAG TTCAAAAGCA 
CCTAAGAAAA ATAGGCTGAG GAGGAAGTCT ACCAGGTGTG TTCTTCCACT CGAACCAATC 
AGTAGAAATC CGAGCCCACC TACTTGTGCT GAACTTCAGA TCGAGAGTTG TGGTAGCAGT 
GAAGAAACAA AGAAAAACAA TTCCAACCAA ACCCCAGCCG GGCACATTAG AGAGCCTCAA 
CTCATCGAAG ACACAGAACC CGCAGCTGAT GCCAAGAAGA ACGAGCCAAA TGAACATATA 
AGGAAGAGAA GTGCCAGTGA TGCGTTCCCA GAAGAGAAAT TAATGAACAA AGCTGGTTTA 
TTAACTAGCT GTTCAAGTCC TAGGAAGCCT CAAGGACCTG TCAATCCTAG CCCTGAGAGA 
AAAGGAATAG AGCAACTTGA AATGTGCCAG ATGCCTGATA ATAACAAAGA ACTCGGGGAT 
TTGGTCCTGG GAGGAGAGCC CAGTGGGAAA CCTACTGAAC CATCTGAGGA GAGCACCAGT 
GTGTCCTTGG TACCCGACAC AGACTACGAC ACCCAGAACA GTGTCTCAAT ACTGGAAGCG 
AACACTGTCA GATATGCAAG AACAGGATCA GTTCAGTGTA TGACTCAGTT TGTCGCAAGT 
GAAAACCCCA AGGAACTTGT CCATGGTTCT AACAATGCTG GAAGTGGCTC GGAGTGCTTC 
AAGCACCCAT TGAGACATGA ACTTAACCAC AATCAAGAGA CAATAGAAAT GGAAGACAGT 
GAACTTGATA CTCAGTATTT GCAGAATACA TTTCAAGTTT CAAAGCGTCA GTCATTTGCT 
TTATTTTCAA AACTTAGAAG TCCCCAAAAG GACTGTACTC TGGTAGGTGC CCGCTCTGTG 
CCCTCAAGGG AACCAAGTCC AAAGGTGACT TCTAGAGGTG AACAAAAAGA ACGTCAGGGA 
CAAGAAGAGT CTGAAATCAG TCATGTACAG GCAGTCACAG TCACAGTAGG CTTACCTGTG 
CCCTGTCAGG AAGGTAAGCC AGGTGCTGTT ACAATGTGTG CTGATGTTTC TAGGCTTTGT 
CCGTCATCTC ATTATAGAAG CTGTGAGAAT GGACTCAACA CCACAGATAA ATCTGGAATT 
TCACAAAACT CACATTTTAG ACAATCAGTT TCTCCCCTCA GGTCATCTAT AAAAACTGAC 
AATAGAAAAA CTCTGACAGA GGGACGATTT GAGAAACATA CTGAAAGGGG AATGGGAAAT 
GAGACTGCTG TTCAAAGTAC AATACACACA ATTAGTCAAA ATAACAGAGG AGATGCTTGT 
CAAGAAGCCA GCTCAGGCAG TGTTATTGAA GTACATTCCA CTGGTGAAAA CGTCCAGGGG 
CAACTAGATA GAAACAGAGG GCCTACGGTA AACACCGTGT CTCTATTAGA TAGTACACAG 
CCTGGTGTCT CTAAGCAGAG TGCTCCTGTA AGTGATAAGT ATCTTGAAAT AAAGCAGGAG 
AGTAAGGCTG TCAGTGCAGA CTTCTCTCCA TGTCTGTTCT CAGATCATCT TGAAAAACCT 
ATGAGAAGTG ATAAGATTTT TCAGGTTTGC TCTGAGACAC CTGATGACCT GTTGGATGAT 
GTTGAAATAC AGGAAAATGC TAGCTTCGGT GAAGGTGGCA TAACGGAAAA GTCTGCTATT 
TTTAATGGAA GTGTCCTGAG AAGAGAGTCC AGTAGGATCC CTAGCCCTGT AACCCATGCA 
TCGAAGTCGC GGAGTCTCCA CAGAGGGTCT AGGAAATTAG AATTCTCAGA AGAGAGCGAC 
TCCACTGAGG ATGAAGACCT TCCCTGCTTC CAGCACTTGC TGAGCAGAGT AAGCAGCACA 
CCCGAACTTA CCAGATGCAG CAGTGTGGTG ACACAGCGTG TGCCAGAGAA AGCGAAGGGG 
ACCCAAGCAC CCCGGAAGAG TAGCATCAGT GACTGCAATA ATGAGGTGAT CTTGGTAGAG 
GCATCTCAGG AATATCAGTT TAGTGAGGAT GCAAAATGCT CTGGCAGCAT GTTCTCCTCA 
CAGCACAGTG CTGCCCTAGG TTCACCTGCA AATGCACTCT CCCAGGATCC CGATTTTAAT 
CCACCTTCCA AACAAAGGCG TCACCAGGCT GAGAATGAGG AAGCCTTTCT AAGTGATAAG 
GAATTGATTT CAGATCATGA GGACATGGCA GCTTGCCTAG AAGAGGCTAG CGATCAAGAA 
GAGGACAGTA TAATCCCGGA CTCAGTGGCG TCTGGATATG AGAGTGAAGC AAACCTCTCT 
GAAGACTGCT CACAGAGTGA TATTCTAACC ACTCAGCAGA GGGCGACCAT GAAGGATAAC 
CTGATAAAGC TCCAGCAGGA AATGGCTCAG CTGGAAGCTG TGCTAGAGCA GCATGGGAGC 
CAGCCGTCTG GCCACCCCCC TTGCCTCCCA GCTGACCCTT GTGCCCTAGA AGACCTGCCA 
GATCCAGAAC AAAACAGATC AGGAACAGCA ATTTTAACTT CAAAGAACAT TAATGAGAAT 
CCTGTAAGCC AGAATCCAAA GCGCGCCTGT GATGACAAAT CCCAACCACA ACCTCCGGAC 
GGTCTCCCCA GTGGAGATAA GGAGTCAGGA ATGAGAAGGC CTTCCCCTTT TAAATCTCCA 
CTGACCAGCA GAAGGTGCTC TGCACGAGGC CACTCCAGGA GTCTTCAGAA TAGAAACTCC 
ACCTCTCAAG AGGAGCTCCT TCAGCCTGTT GAATTGGAGA AGTCATGTGA ACCACACAAC 
TTAACAGGGC GGTCTTGCTT GCCAAGGCAA GATCTCGAAG GAACCCCATA CCTGGAATCT 
GGAATCAGCC TTTTCTCCAG TAGAGACCCC GATTCTGAAT CCCCTAAAGT GCCAGCCCTT 
GTTTGCACTG CACCAGCTTC AACCTCTGCA CTGAAAATAT CCCAAGGTCA AGTTGCTGGG 
TCTTGCAGGA GTCCAGCTGC TGGTGGTGCT GATACTGCAG TGGTAGAAAT TGTGAGCAAG 
ATAAAGCCGG AAGTGACGTC TTCAAAAGAA CGAGCGGAGA GAGATATATC CATGGTGGTG 
TCAGGCTTGA CCCCTAAAGA AGTAATGATT GTGCAAAAGT TTGCTGAAAA ATACCGCCTT 
GCTTTAACTG ATGTAATTAC TGAGGAAACT ACGCATGTAA TTATAAAAAC AGATGCTGAG 
TTTGTGTGTG AACGGACACT GAAATATTTT CTGGGCATTG CAGGAGGAAA GTGGATAGTT 
AGCTATTCTT GGGTGATCAA GTCTATCCAA GAAAGAAAAC TTCTGAGTGT GCATGAATTT 
GAAGTCAAAG GAGACGTTGT GACTGGAAGC AACCACCAAG GTCCAAGGCG ATCCAGAGAA 
TCCCAGGAAA AGCTCTTTGA AGGCCTACAG ATCTATTGTT GTGAGCCCTT CACCAACATG 
CCCAAAGATG AGCTGGAGAG GATGCTGCAG CTGTGTGGGG CTTCTGTGGT GAAGGAGCTT 
CCATTGCTCA CCCGTGACAC AGGTGCTCAT CCAATTGTGC TCGTGCAGCC AAGTGCCTGG 
ACAGAAGACA ACGACTGCCC TGATATTGGG CAGCTGTGCA AGGGACGTCT AGTGATGTGG 
GACTGGGTGT TGGACAGTAT ATCCGTCTAC CGGTGTCGGG ATCTGGATGC CTACCTGGTA 
CAGAATATCA CCTGTGGCCG TGATGGTAGC GAGCCACAAG ACTCCAATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766314.1
CDS	418..5871
Translation	MDLSAVRIQEVQNVLHAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNEITKRSLQGSARFSQLVEELLKIIDAFELDTGMQCANGFSFSKKKNSSSELLNEDASIIQSVGYRNRVKKLRQIESGSATLKDSLSVQLSNLGIVRSMKKNRQTQPQNKSVYIALESDSSEERVNAPDGCSVRDQELFQIAPGGAGDEGKLNSAKKAACDFSEGIRNIEHHQCSDKDLNPTENHATERHPEKCPRISVANVHVEPCGTDARASSLQRGTRSLLFTEDRLDAEKAEFCDRSKQSGAAVSQQSRWADSKETCNGRPVPRTEGKADPNVDSLCGRKQWNHPKSLCPENSGATTDVPWITLNSSIQKVNEWFSRTGEMLTSDNASDRRPASNAEAAVVLEVSNEVDGCFSSSKKIDLVAPDSDNAVMCTSGRDFSKPVENIINDKIFGKTYQRKGSRPHLNHVTEIIGTFTTEPQIIQEQPFTNKLKRKRSTCLHPEDFIKKADLTVVQRISENLNQGTDQMEPNDQAMSITSNGQENRATGNDLQRGRNAHPIESLRKEPAFTAKAKSISNSISDLEVELNVHSSKAPKKNRLRRKSTRCVLPLEPISRNPSPPTCAELQIESCGSSEETKKNNSNQTPAGHIREPQLIEDTEPAADAKKNEPNEHIRKRSASDAFPEEKLMNKAGLLTSCSSPRKPQGPVNPSPERKGIEQLEMCQMPDNNKELGDLVLGGEPSGKPTEPSEESTSVSLVPDTDYDTQNSVSILEANTVRYARTGSVQCMTQFVASENPKELVHGSNNAGSGSECFKHPLRHELNHNQETIEMEDSELDTQYLQNTFQVSKRQSFALFSKLRSPQKDCTLVGARSVPSREPSPKVTSRGEQKERQGQEESEISHVQAVTVTVGLPVPCQEGKPGAVTMCADVSRLCPSSHYRSCENGLNTTDKSGISQNSHFRQSVSPLRSSIKTDNRKTLTEGRFEKHTERGMGNETAVQSTIHTISQNNRGDACQEASSGSVIEVHSTGENVQGQLDRNRGPTVNTVSLLDSTQPGVSKQSAPVSDKYLEIKQESKAVSADFSPCLFSDHLEKPMRSDKIFQVCSETPDDLLDDVEIQENASFGEGGITEKSAIFNGSVLRRESSRIPSPVTHASKSRSLHRGSRKLEFSEESDSTEDEDLPCFQHLLSRVSSTPELTRCSSVVTQRVPEKAKGTQAPRKSSISDCNNEVILVEASQEYQFSEDAKCSGSMFSSQHSAALGSPANALSQDPDFNPPSKQRRHQAENEEAFLSDKELISDHEDMAACLEEASDQEEDSIIPDSVASGYESEANLSEDCSQSDILTTQQRATMKDNLIKLQQEMAQLEAVLEQHGSQPSGHPPCLPADPCALEDLPDPEQNRSGTAILTSKNINENPVSQNPKRACDDKSQPQPPDGLPSGDKESGMRRPSPFKSPLTSRRCSARGHSRSLQNRNSTSQEELLQPVELEKSCEPHNLTGRSCLPRQDLEGTPYLESGISLFSSRDPDSESPKVPALVCTAPASTSALKISQGQVAGSCRSPAAGGADTAVVEIVSKIKPEVTSSKERAERDISMVVSGLTPKEVMIVQKFAEKYRLALTDVITEETTHVIIKTDAEFVCERTLKYFLGIAGGKWIVSYSWVIKSIQERKLLSVHEFEVKGDVVTGSNHQGPRRSRESQEKLFEGLQIYCCEPFTNMPKDELERMLQLCGASVVKELPLLTRDTGAHPIVLVQPSAWTEDNDCPDIGQLCKGRLVMWDWVLDSISVYRCRDLDAYLVQNITCGRDGSEPQDSND

Target ORF information:

RefSeq Version	XM_008768092.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus BRCA1, DNA repair associated (Brca1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768092.2

ORF Insert Sequence:

ATGGATTTAT CTGCTGTTCG AATTCAAGAA GTACAAAATG TCCTTCATGC TATGCAGAAA 
ATCTTGGAGT GTCCAATCTG TTTGGAACTG ATCAAAGAAC CGGTTTCCAC AAAGTGCGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTCCTTAACC AGAAGAAAGG ACCTTCCCAG 
TGTCCTTTGT GTAAGAATGA GATAACCAAA AGGAGCCTAC AAGGAAGTGC AAGGTTTAGT 
CAACTTGTTG AAGAGCTGCT GAAAATCATT GATGCTTTTG AGCTTGACAC CGGAATGCAG 
TGTGCAAATG GTTTTAGTTT TTCAAAAAAG AAAAATAGTT CTTCTGAGCT TCTGAATGAG 
GACGCTTCTA TCATCCAGAG TGTGGGCTAC CGAAACCGCG TCAAAAAACT TCGACAGATT 
GAGTCTGGAA GTGCCACCTT GAAGGACAGT CTCAGTGTCC AGTTGTCTAA CCTTGGGATT 
GTGAGATCAA TGAAGAAAAA TAGGCAGACA CAACCTCAAA ACAAATCTGT CTACATTGCT 
TTAGAATCTG ATTCTTCTGA GGAGAGAGTA AATGCACCCG ATGGCTGCAG TGTGAGAGAT 
CAGGAATTGT TTCAGATCGC CCCTGGAGGA GCTGGAGATG AAGGCAAGTT GAACTCTGCA 
AAAAAGGCTG CTTGTGACTT TTCTGAGGGC ATAAGAAACA TTGAACATCA TCAATGCAGT 
GATAAAGATT TGAACCCCAC TGAGAATCAT GCAACTGAAA GGCATCCAGA AAAATGTCCG 
CGTATTTCTG TTGCAAACGT GCACGTGGAG CCGTGTGGCA CAGATGCTCG TGCCAGCTCA 
TTACAGCGTG GGACCCGCAG TTTATTGTTC ACTGAGGACA GACTGGATGC AGAAAAGGCT 
GAATTCTGTG ATAGAAGCAA ACAGTCTGGC GCAGCAGTGA GCCAGCAGAG CAGATGGGCT 
GACAGTAAAG AAACATGTAA TGGCAGGCCG GTTCCCCGCA CTGAGGGAAA GGCAGATCCA 
AATGTGGATT CCCTCTGTGG TAGAAAGCAG TGGAATCATC CGAAAAGCCT GTGCCCTGAG 
AATTCTGGAG CTACCACTGA CGTTCCTTGG ATAACACTGA ATAGCAGCAT TCAGAAAGTG 
AATGAGTGGT TTTCCAGAAC TGGTGAAATG TTAACTTCTG ACAATGCATC TGACAGGAGG 
CCTGCGTCAA ATGCAGAAGC TGCTGTTGTG TTAGAAGTTT CAAATGAAGT GGATGGATGT 
TTCAGTTCTT CAAAGAAAAT AGACTTAGTT GCCCCTGATT CCGATAATGC TGTAATGTGT 
ACAAGTGGAA GAGACTTCTC CAAGCCAGTA GAGAATATTA TCAACGATAA AATATTTGGG 
AAAACCTATC AGAGAAAGGG AAGCCGCCCT CACTTGAACC ATGTGACTGA AATTATAGGC 
ACATTTACTA CAGAACCACA GATTATACAA GAGCAGCCCT TCACAAATAA ATTAAAACGC 
AAAAGAAGTA CATGCCTTCA TCCTGAGGAC TTTATCAAGA AAGCAGATTT AACAGTTGTT 
CAAAGGATTT CTGAAAATTT AAATCAGGGA ACTGACCAAA TGGAGCCAAA TGACCAAGCA 
ATGAGTATTA CCAGTAACGG TCAGGAGAAC AGAGCAACAG GTAATGATCT TCAGAGAGGG 
AGAAATGCTC ATCCAATAGA ATCATTGAGA AAGGAACCTG CTTTCACAGC TAAAGCCAAA 
TCTATAAGCA ACAGTATAAG TGATTTGGAG GTAGAATTAA ATGTTCACAG TTCAAAAGCA 
CCTAAGAAAA ATAGGCTGAG GAGGAAGTCT ACCAGGTGTG TTCTTCCACT CGAACCAATC 
AGTAGAAATC CGAGCCCACC TACTTGTGCT GAACTTCAGA TCGAGAGTTG TGGTAGCAGT 
GAAGAAACAA AGAAAAACAA TTCCAACCAA ACCCCAGCCG GGCACATTAG AGAGCCTCAA 
CTCATCGAAG ACACAGAACC CGCAGCTGAT GCCAAGAAGA ACGAGCCAAA TGAACATATA 
AGGAAGAGAA GTGCCAGTGA TGCGTTCCCA GAAGAGAAAT TAATGAACAA AGCTGGTTTA 
TTAACTAGCT GTTCAAGTCC TAGGAAGCCT CAAGGACCTG TCAATCCTAG CCCTGAGAGA 
AAAGGAATAG AGCAACTTGA AATGTGCCAG ATGCCTGATA ATAACAAAGA ACTCGGGGAT 
TTGGTCCTGG GAGGAGAGCC CAGTGGGAAA CCTACTGAAC CATCTGAGGA GAGCACCAGT 
GTGTCCTTGG TACCCGACAC AGACTACGAC ACCCAGAACA GTGTCTCAAT ACTGGAAGCG 
AACACTGTCA GATATGCAAG AACAGGATCA GTTCAGTGTA TGACTCAGTT TGTCGCAAGT 
GAAAACCCCA AGGAACTTGT CCATGGTTCT AACAATGCTG GAAGTGGCTC GGAGTGCTTC 
AAGCACCCAT TGAGACATGA ACTTAACCAC AATCAAGAGA CAATAGAAAT GGAAGACAGT 
GAACTTGATA CTCAGTATTT GCAGAATACA TTTCAAGTTT CAAAGCGTCA GTCATTTGCT 
TTATTTTCAA AACTTAGAAG TCCCCAAAAG GACTGTACTC TGGTAGGTGC CCGCTCTGTG 
CCCTCAAGGG AACCAAGTCC AAAGGTGACT TCTAGAGGTG AACAAAAAGA ACGTCAGGGA 
CAAGAAGAGT CTGAAATCAG TCATGTACAG GCAGTCACAG TCACAGTAGG CTTACCTGTG 
CCCTGTCAGG AAGGTAAGCC AGGTGCTGTT ACAATGTGTG CTGATGTTTC TAGGCTTTGT 
CCGTCATCTC ATTATAGAAG CTGTGAGAAT GGACTCAACA CCACAGATAA ATCTGGAATT 
TCACAAAACT CACATTTTAG ACAATCAGTT TCTCCCCTCA GGTCATCTAT AAAAACTGAC 
AATAGAAAAA CTCTGACAGA GGGACGATTT GAGAAACATA CTGAAAGGGG AATGGGAAAT 
GAGACTGCTG TTCAAAGTAC AATACACACA ATTAGTCAAA ATAACAGAGG AGATGCTTGT 
CAAGAAGCCA GCTCAGGCAG TGTTATTGAA GTACATTCCA CTGGTGAAAA CGTCCAGGGG 
CAACTAGATA GAAACAGAGG GCCTACGGTA AACACCGTGT CTCTATTAGA TAGTACACAG 
CCTGGTGTCT CTAAGCAGAG TGCTCCTGTA AGTGATAAGT ATCTTGAAAT AAAGCAGGAG 
AGTAAGGCTG TCAGTGCAGA CTTCTCTCCA TGTCTGTTCT CAGATCATCT TGAAAAACCT 
ATGAGAAGTG ATAAGATTTT TCAGGTTTGC TCTGAGACAC CTGATGACCT GTTGGATGAT 
GTTGAAATAC AGGAAAATGC TAGCTTCGGT GAAGGTGGCA TAACGGAAAA GTCTGCTATT 
TTTAATGGAA GTGTCCTGAG AAGAGAGTCC AGTAGGATCC CTAGCCCTGT AACCCATGCA 
TCGAAGTCGC GGAGTCTCCA CAGAGGGTCT AGGAAATTAG AATTCTCAGA AGAGAGCGAC 
TCCACTGAGG ATGAAGACCT TCCCTGCTTC CAGCACTTGC TGAGCAGAGT AAGCAGCACA 
CCCGAACTTA CCAGATGCAG CAGTGTGGTG ACACAGCGTG TGCCAGAGAA AGCGAAGGGG 
ACCCAAGCAC CCCGGAAGAG TAGCATCAGT GACTGCAATA ATGAGGTGAT CTTGGTAGAG 
GCATCTCAGG AATATCAGTT TAGTGAGGAT GCAAAATGCT CTGGCAGCAT GTTCTCCTCA 
CAGCACAGTG CTGCCCTAGG TTCACCTGCA AATGCACTCT CCCAGGATCC CGATTTTAAT 
CCACCTTCCA AACAAAGGCG TCACCAGGCT GAGAATGAGG AAGCCTTTCT AAGTGATAAG 
GAATTGATTT CAGATCATGA GGACATGGCA GCTTGCCTAG AAGAGGCTAG CGATCAAGAA 
GAGGACAGTA TAATCCCGGA CTCAGTGGCG TCTGGATATG AGAGTGAAGC AAACCTCTCT 
GAAGACTGCT CACAGAGTGA TATTCTAACC ACTCAGCAGA GGGCGACCAT GAAGGATAAC 
CTGATAAAGC TCCAGCAGGA AATGGCTCAG CTGGAAGCTG TGCTAGAGCA GCATGGGAGC 
CAGCCGTCTG GCCACCCCCC TTGCCTCCCA GCTGACCCTT GTGCCCTAGA AGACCTGCCA 
GATCCAGAAC AAAACAGATC AGGAACAGCA ATTTTAACTT CAAAGAACAT TAATGAGAAT 
CCTGTAAGCC AGAATCCAAA GCGCGCCTGT GATGACAAAT CCCAACCACA ACCTCCGGAC 
GGTCTCCCCA GTGGAGATAA GGAGTCAGGA ATGAGAAGGC CTTCCCCTTT TAAATCTCCA 
CTGACCAGCA GAAGGTGCTC TGCACGAGGC CACTCCAGGA GTCTTCAGAA TAGAAACTCC 
ACCTCTCAAG AGGAGCTCCT TCAGCCTGTT GAATTGGAGA AGTCATGTGA ACCACACAAC 
TTAACAGGGC GGTCTTGCTT GCCAAGGCAA GATCTCGAAG GAACCCCATA CCTGGAATCT 
GGAATCAGCC TTTTCTCCAG TAGAGACCCC GATTCTGAAT CCCCTAAAGT GCCAGCCCTT 
GTTTGCACTG CACCAGCTTC AACCTCTGCA CTGAAAATAT CCCAAGGTCA AGTTGCTGGG 
TCTTGCAGGA GTCCAGCTGC TGGTGGTGCT GATACTGCAG TGGTAGAAAT TGTGAGCAAG 
ATAAAGCCGG AAGTGACGTC TTCAAAAGAA CGAGCGGAGA GAGATATATC CATGGTGGTG 
TCAGGCTTGA CCCCTAAAGA AGTAATGATT GTGCAAAAGT TTGCTGAAAA ATACCGCCTT 
GCTTTAACTG ATGTAATTAC TGAGGAAACT ACGCATGTAA TTATAAAAAC AGATGCTGAG 
TTTGTGTGTG AACGGACACT GAAATATTTT CTGGGCATTG CAGGAGGAAA GTGGATAGTT 
AGCTATTCTT GGGTGATCAA GTCTATCCAA GAAAGAAAAC TTCTGAGTGT GCATGAATTT 
GAAGTCAAAG GAGACGTTGT GACTGGAAGC AACCACCAAG GTCCAAGGCG ATCCAGAGAA 
TCCCAGGAAA AGCTCTTTGA AGGCCTACAG ATCTATTGTT GTGAGCCCTT CACCAACATG 
CCCAAAGATG AGCTGGAGAG GATGCTGCAG CTGTGTGGGG CTTCTGTGGT GAAGGAGCTT 
CCATTGCTCA CCCGTGACAC AGGTGCTCAT CCAATTGTGC TCGTGCAGCC AAGTGCCTGG 
ACAGAAGACA ACGACTGCCC TGATATTGGG CAGCTGTGCA AGGGACGTCT AGTGATGTGG 
GACTGGGTGT TGGACAGTAT ATCCGTCTAC CGGTGTCGGG ATCTGGATGC CTACCTGGTA 
CAGAATATCA CCTGTGGCCG TGATGGTAGC GAGCCACAAG ACTCCAATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A BRCA1-Dependent DNA Damage Response in the Regenerating Adult Peripheral Nerve Milieu.
Molecular neurobiology(2017 Jun)
Krishnan A,Purdy K,Chandrasekhar A,Martinez J,Cheng C,Zochodne DW

NPR3 protects cardiomyocytes from apoptosis through inhibition of cytosolic BRCA1 and TNF-?.
Cell cycle (Georgetown, Tex.)15(18)2414-9(2016 Sep)
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Brca1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

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